DOCSLIB.ORG

Etude De La Diversité De Pectobacterium Spp Et Des Effets Induits Par Les Lipopolysaccharides Chez Les Plantes Mohamed Kettani Halabi

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Etude De La Diversité De Pectobacterium Spp Et Des Effets Induits Par Les Lipopolysaccharides Chez Les Plantes Mohamed Kettani Halabi Etude de la diversité de Pectobacterium spp et des effets induits par les lipopolysaccharides chez les plantes Mohamed Kettani Halabi To cite this version: Mohamed Kettani Halabi. Etude de la diversité de Pectobacterium spp et des effets induits par les lipopolysaccharides chez les plantes. Sciences agricoles. Université Paris Sud - Paris XI; Univer- sité Hassan II (Mohammedia, Maroc). Faculté des sciences et techniques, 2012. Français. NNT : 2012PA112230. tel-01070631 HAL Id: tel-01070631 https://tel.archives-ouvertes.fr/tel-01070631 Submitted on 2 Oct 2014 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. UNIVERSITE PARIS-SUD – UFR des Sciences ECOLE DOCTORALE SCIENCES DU VEGETAL ED 145 UNIVERSITE HASSAN II MOHAMMEDIA - CASABLANCA Thèse de Doctorat Pour obtenir le grade de DOCTEUR EN SCIENCES DE L’UNIVERSITE PARIS-SUD et DOCTEUR DE L’UNIVERSITE HASSAN II MOHAMMEDIA – CASABLANCA Présentée par : M. Mohamed KETTANI HALABI Spécialité : Biologie Sujet de la thèse : Etude de la diversité de Pectobacterium spp et des effets induits par les lipopolysaccharides chez les plantes Thèse présentée et soutenue à Mohammedia le 22 septembre 2012 devant le jury composé de : Michel DRON Professeur d’Enseignement Supérieur Président Directeur de l'IBP – Paris, France Mouncef BENCHEKROUN Professeur d’Enseignement Supérieur Rapporteur Directeur du CED -FST Settat, Maroc Christian MAZARS Directeur de Recherche Rapporteur Université Paul Sabatier -CNRS- Toulouse- France Rajaa Ait MHAND Professeur Habilité Examinateur Université Hassan II Mohammedia, Maroc François BOUTEAU Maître de Conférences Directeur de thèse Université Paris-Diderot 7, Paris, France Moulay Mustapha ENNAJI Professeur d’Enseignement Supérieur Directeur de thèse Université Hassan II Mohammedia, Maroc Etablissements : Faculté des Sciences et Technique Mohammedia Faculté des Sciences d’Orsay CEDocs : Sciences, Techniques, Ingénierie et Développement Durable Sciences du Végétal ED 145 Noms des laboratoires : Laboratoire de Virologie et Hygiène & Microbiologie (LVHM) Laboratoire d’Electrophysiologie des Membranes (LEM) … Thèse de Doctorat Ès Sciences Productions scientifiques PRODUCTIONS SCIENTIFIQUES A. Articles Scientifiques (08) A.1. Articles Scientifiques Internationaux (04) Kettani-Halabi, M.*, Terta, M.*, Ibenyassine, K., Tran, D., Meimoun, P., M'Hand, R.A., El- Maarouf-Bouteau, H., Val, F., Ennaji, M.M., and Bouteau, F. 2010. Arabidopsis thaliana Cells: A Model to Evaluate the Virulence of Pectobacterium carotovorum. Molecular Plant-Microbe Interactions 23:139-143. (* Co-auteur) Baz M., Tran D., Kettani-Halabi M., Samri S.E., Jamjari A., Biligui B., Meimoun P., El- Maarouf-Bouteau H., Garmier M., Saindrenan P., Ennaji M.M., Barakate M., and Bouteau F. 2012. Calcium and ROS mediated defense responses in BY2 tobacco cells by non-pathogenic Streptomyces sp. Journal of Applied Microbiology 112:782-792. Kettani-Halabi M., Tran D, Dauphin A, Errakhi R, Val F, Ennaji MM, Bouteau F. Early common and differential responses of Arabidopsis thaliana cells to lipopolysaccharides from pathogenic and non-pathogenic bacteria (Submitted in Journal of Experimental Botany). Kettani-Halabi M, Terta M, Amdan M, Elfahim M, Bouteau F, Ennaji MM. Sequence analysis of a pmrA gene as a tool to identify Pectobacterium carotovorum subsp. carotovorum in relation to potato diseases (Submitted in BMC Microbiology). A.2 Articles Scientifiques Nationaux (04) Kettani-Halabi M, Amdan M, Terta M, Tran D, Yekkour A, Val F, Ennaji MM, Bouteau F. 2012 Les lipopolysaccharides, biostimulants pour la protection de la pomme de terre contre Pectobacterium carotovorum subsp. carotovorum. Moroccan journal of plant protection 4:131- 140. Terta M., Spirada U., Faouzi A., Melloul, M., El Fafime El., Amdan M., Kettani-Halabi M., Ait M’hand R., Achbani El., Barakate M., Bouteau F., Ennaji MM. 2012. Pectobacterium carotovorum subsp. brasiliensis associée à la pourriture molle de la pomme de terre : une première mise en évidence au Maroc. Moroccan journal of plant protection 4:107-118. Amdan M., Ennaji MM., Terta M., Kettani-Halabi M., Faqihi H., Barakat M., Ait Mhand R. L’agressivité de Pectobacterium carotovorum, agent responsable de la pourriture molle de la pomme de terre. Moroccan journal of plant protection 4:97-106. Amdan M., Terta M., Kettani-Halabi M., Achbani El., Ennaji MM. 2012. Pathogénicité de Pectobacterium sp. Agent responsable de la pourriture molle de la pomme de terre. Dans Ennemis de la culture de pomme de terre au Maroc. Edition Dr El Hassan Achbani. Septembre 2011 pp 39-49. M. KETTANI HALABI iii Thèse de Doctorat Ès Sciences Productions scientifiques B. Communications Orales (04) Kettani-Halabi M, Tran D, Amdan M, Terta M, Val F, Ennaji MM, Bouteau F. Implication des Lipopolysaccharides (LPS) dans la stimulation de la défense chez Arabidopsis thaliana. 4ème Journée Scientifique Nationale de l’Environnement et de la Santé « Impact de l’Environnement sur la Santé Public » le 02 Juin 2011 à la FSTM, Maroc ; Kettani-Halabi M, Tran D, Terta M, Amdan M, Val F, Ennaji MM, Bouteau F. Rôle des Lipopolysaccharides (LPS) dans l’induction des mécanismes de défense chez Arabidopsis thaliana, nouvelle approche pour lutter contre le Pectobacterium spp. Congrès International « La Recherche, la Biotechnologie et le Consommateur au service de l’Environnement et de l’Industrie Agroalimentaire" le 19 et 20 Mai 2011 à la Faculté des Sciences de Kénitra, Maroc ; Kettani-Halabi M, Terta M, Ibenyassine K, Tran D, Meimoun P, M’hand RA, El-Maarouf- Bouteau H, Val F, Ennaji MM, Bouteau F. Arabidopsis thaliana cells: a model to evaluate the virulence of Pectobacterium carotovorum. Congrès International MICROBIOD-2009 « Biotechnologie microbienne au service du développement » tenu le 02-05 Novembre 2009 à Marrakech – Maroc ; Ennaji MM, Ait M Hand R, Achabni E, Terta M, Kettani-Halabi M, Baz M, El karkouri A, El Hassouni M, Maaroufi H, Val F, Bouteau F, Barakate M. Caractérisation de la biodiversité de la bactérie macérgéne Erwinia sp. au Maroc. Congrès International MICROBIOD-2009 « Biotechnologie microbienne au service du développement » tenu le 02-05 Novembre 2009 à Marrakech – Maroc. C. Communications Affichées (07) Kettani-Halabi M, Tran D, Terta M, M’hand RA, Val F, Ennaji MM, Bouteau F. Use of Arabidopsis thaliana cells to analyse the pathogenicity of Pectobacterium carotovorum. Congrès de la Société Française de Phytopathologie 2012 du 05 et 8 juin 2012, Paris – France. Yekkour A, Tran D, Kettani-Halabi M, Mathieu F, Briand J, Errakhi R, Lebrihi A, Sabaou N, Bouteau F. effect of Fusarium mycotoxin deoxynivalenol on Nicotiana tabacum cells: signaling events leading to PCD. Congrès de la Société Française de Phytopathologie 2012 du 05 et 8 juin 2012, Paris – France. Kettani-Halabi M, Tran D, Val F, Ennaji MM, Bouteau F. Cell signaling induced by bacterial LPS in Arabidopsis thaliana. The First International Meeting on Ion Channel Signaling Mechanisms: from basic science to clinical application. October 31-November 4, 2011 Marrakesh, Morocco. Kettani-Halabi M, Terta M, Amdan M, M’hand RA, Barakate M, Achbani E, Val F, Bouteau F, Ennaji MM. Virulence et Diversité Génétique du Pouvoir Pathogène des Souches de Pectobacterium carotovorum Isolées des Pommes de Terre Marocaines. Congrès International « La Recherche, la Biotechnologie et le Consommateur au service de l’Environnement et de l’Industrie Agroalimentaire » du 19 et 20 mai 2011 de la Faculté des Sciences de Kénitra, Maroc. Kettani-Halabi M, Tran D, Terta M, Amdan M, Val F, Ennaji MM, Bouteau F. Mechanisms involved in cell signaling in response to various LPS in Arabidopsis thaliana. Congrès International « La Recherche, la Biotechnologie et le Consommateur au service de M. KETTANI HALABI iv Thèse de Doctorat Ès Sciences Productions scientifiques l’Environnement et de l’Industrie Agroalimentaire » du 19 et 20 mai 2011 de la Faculté des Sciences de Kénitra, Maroc. Kettani-Halabi M, Terta M, Amdan M, M’hand RA, Barakate M, Achbani E, Val F, Bouteau F, Ennaji MM. Pectobacterium carotovorum de la Pomme de Terre Marocaine: Nouvelle Nomenclature pour Erwinia carotovora subsp. carotovora, Symptomatologie, Sources et Facteurs de Contamination. 1er Journée de l’Eau sous le thème « Eau et Santé » tenu le 27 Mai 2010 Casablanca, Hôtel Riad Salam, Maroc ; Amdan M, Terta M, Kettani-halabi M, Ait M’hand R, Ennaji MM. Biodiversité de la bactérie Erwinia sp. responsable de la pourriture molle de la pomme de terre au Maroc. 1er Journée de l’Eau sous le thème « Eau et Santé » tenu le 27 Mai 2010 Casablanca, Hôtel Riad Salam, Maroc. D. Publication à Gene Bank (NCBI) de 30 séquences du gène pmrA du Pectobacterium Kettani-Halabi M, Terta M, Amdan M, Melloul M, El Alaoui A, El Fahime E.M, Bouteau F and Ennaji M.M. 2012. Sequence analysis of the gene encoding polymyxin resistance (PmrA) from different strains of Pectobacterium carotovorum subsp. carotovorum. PopSet: 383549958 M. KETTANI HALABI v Thèse de Doctorat Ès Sciences Productions scientifiques M. KETTANI HALABI vi Thèse de Doctorat Ès Sciences Formations
Load more

Recommended publications
  • Light-Induced Psba Translation in Plants Is Triggered by Photosystem II Damage Via an Assembly-Linked Autoregulatory Circuit
    Light-induced psbA translation in plants is triggered by photosystem II damage via an assembly-linked autoregulatory circuit Prakitchai Chotewutmontria and Alice Barkana,1 aInstitute of Molecular Biology, University of Oregon, Eugene, OR 97403 Edited by Krishna K. Niyogi, University of California, Berkeley, CA, and approved July 22, 2020 (received for review April 26, 2020) The D1 reaction center protein of photosystem II (PSII) is subject to mRNA to provide D1 for PSII repair remain obscure (13, 14). light-induced damage. Degradation of damaged D1 and its re- The consensus view in recent years has been that psbA transla- placement by nascent D1 are at the heart of a PSII repair cycle, tion for PSII repair is regulated at the elongation step (7, 15–17), without which photosynthesis is inhibited. In mature plant chloro- a view that arises primarily from experiments with the green alga plasts, light stimulates the recruitment of ribosomes specifically to Chlamydomonas reinhardtii (Chlamydomonas) (18). However, we psbA mRNA to provide nascent D1 for PSII repair and also triggers showed recently that regulated translation initiation makes a a global increase in translation elongation rate. The light-induced large contribution in plants (19). These experiments used ribo- signals that initiate these responses are unclear. We present action some profiling (ribo-seq) to monitor ribosome occupancy on spectrum and genetic data indicating that the light-induced re- cruitment of ribosomes to psbA mRNA is triggered by D1 photo- chloroplast open reading frames (ORFs) in maize and Arabi- damage, whereas the global stimulation of translation elongation dopsis upon shifting seedlings harboring mature chloroplasts is triggered by photosynthetic electron transport.
    [Show full text]
  • Evolution of Photochemical Reaction Centres
    bioRxiv preprint doi: https://doi.org/10.1101/502450; this version posted December 20, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Evolution of photochemical reaction 2 centres: more twists? 3 4 Tanai Cardona, A. William Rutherford 5 Department of Life Sciences, Imperial College London, London, UK 6 Correspondence to: [email protected] 7 8 Abstract 9 The earliest event recorded in the molecular evolution of photosynthesis is the structural and 10 functional specialisation of Type I (ferredoxin-reducing) and Type II (quinone-reducing) reaction 11 centres. Here we point out that the homodimeric Type I reaction centre of Heliobacteria has a Ca2+- 12 binding site with a number of striking parallels to the Mn4CaO5 cluster of cyanobacterial 13 Photosystem II. This structural parallels indicate that water oxidation chemistry originated at the 14 divergence of Type I and Type II reaction centres. We suggests that this divergence was triggered by 15 a structural rearrangement of a core transmembrane helix resulting in a shift of the redox potential 16 of the electron donor side and electron acceptor side at the same time and in the same redox direction. 17 18 Keywords 19 Photosynthesis, Photosystem, Water oxidation, Oxygenic, Anoxygenic, Reaction centre 20 21 Evolution of Photosystem II 22 There is no consensus on when and how oxygenic photosynthesis originated. Both the timing and the 23 evolutionary mechanism are disputed.
    [Show full text]
  • Etude Des Sources De Carbone Et D'énergie Pour La Synthèse Des Lipides De Stockage Chez La Microalgue Verte Modèle Chlamydo
    Aix Marseille Université L'Ecole Doctorale 62 « Sciences de la Vie et de la Santé » Etude des sources de carbone et d’énergie pour la synthèse des lipides de stockage chez la microalgue verte modèle Chlamydomonas reinhardtii Yuanxue LIANG Soutenue publiquement le 17 janvier 2019 pour obtenir le grade de « Docteur en biologie » Jury Professor Claire REMACLE, Université de Liège (Rapporteuse) Dr. David DAUVILLEE, CNRS Lille (Rapporteur) Professor Stefano CAFFARRI, Aix Marseille Université (Examinateur) Dr. Gilles PELTIER, CEA Cadarache (Invité) Dr. Yonghua LI-BEISSON, CEA Cadarache (Directeur de thèse) 1 ACKNOWLEDGEMENTS First and foremost, I would like to express my sincere gratitude to my advisor Dr. Yonghua Li-Beisson for the continuous support during my PhD study and also gave me much help in daily life, for her patience, motivation and immense knowledge. I could not have imagined having a better mentor. I’m also thankful for the opportunity she gave me to conduct my PhD research in an excellent laboratory and in the HelioBiotec platform. I would also like to thank another three important scientists: Dr. Gilles Peltier (co- supervisor), Dr. Fred Beisson and Dr. Pierre Richaud who helped me in various aspects of the project. I’m not only thankful for their insightful comments, suggestion, help and encouragement, but also for the hard question which incented me to widen my research from various perspectives. I would also like to thank collaboration from Fantao, Emmannuelle, Yariv, Saleh, and Alisdair. Fantao taught me how to cultivate and work with Chlamydomonas. Emmannuelle performed bioinformatic analyses. Yariv, Saleh and Alisdair from Potsdam for amino acid analysis.
    [Show full text]
  • Chapter 3 the Title and Subtitle of This Chapter Convey a Dual Meaning
    3.1. Introduction Chapter 3 The title and subtitle of this chapter convey a dual meaning. At first reading, the subtitle Photosynthetic Reaction might seem to indicate that the topic of the structure, function and organization of Centers: photosynthetic reaction centers is So little time, so much to do exceedingly complex and that there is simply insufficient time or space in this brief article to cover the details. While this is John H. Golbeck certainly the case, the subtitle is Department of Biochemistry additionally meant to convey the idea that there is precious little time after the and absorption of a photon to accomplish the Molecular Biology task of preserving the energy in the form of The Pennsylvania State University stable charge separation. University Park, PA 16802 USA The difficulty is there exists a fundamental physical limitation in the amount of time available so that a photochemically induced excited state can be utilized before the energy is invariably wasted. Indeed, the entire design philosophy of biological reaction centers is centered on overcoming this physical, rather than chemical or biological, limitation. In this chapter, I will outline the problem of conserving the free energy of light-induced charge separation by focusing on the following topics: 3.2. Definition of the problem: the need to stabilize a charge-separated state. 3.3. The bacterial reaction center: how the cofactors and proteins cope with this problem in a model system. 3.4. Review of Marcus theory: what governs the rate of electron transfer in proteins? 3.5. Photosystem II: a variation on a theme of the bacterial reaction center.
    [Show full text]
  • Lecture Inhibition of Photosynthesis Inhibition at Photosystem I
    1 Lecture Inhibition of Photosynthesis Inhibition at Photosystem I 1. General Information The popular misconception is that susceptible plants treated with these herbicides “starve to death” because they can no longer photosynthesize. In actuality, the plants die long before the food reserves are depleted. The photosynthetic inhibitors can be divided into two distinct groups, the inhibitors of Photosystem I and inhibitors of Photosystem II. Both of these groups work in the energy production step of photosynthesis, or the light reactions. Light is required as well as photosynthesis for these herbicides to kill susceptible plants. Herbicides that inhibit Photosystem I are considered to be contact herbicides and are often referred to as membrane disruptors. The end result is that cell membranes are rapidly destroyed resulting in leakage of cell contents into the intercellular spaces. These herbicides act as “electron interceptors” or “electron thieves” within Photosystem I of the light reaction of photosynthesis. They divert electrons from the normal electron transport sequence necessary in Photosystem I. This in turn inhibits photosynthesis. The membrane disruption occurs as a result of secondary responses. Herbicides that inhibit Photosystem I are represented by only one family, the bipyridyliums. See chemical structure shown under herbicide families. These molecules are cationic (positively charged) and are therefore highly water soluble. Their cationic properties also make them highly adsorbed to soil colloids resulting in no soil activity. 2. Mode of Action See Figure 7.1 (The electron transport chain in photosynthesis and the sites of action of herbicides that interfere with electron transfer in this chain (Q = electron acceptor; PQ = plastoquinone).
    [Show full text]
  • Photosystem I-Based Applications for the Photo-Catalyzed Production of Hydrogen and Electricity
    University of Tennessee, Knoxville TRACE: Tennessee Research and Creative Exchange Doctoral Dissertations Graduate School 12-2014 Photosystem I-Based Applications for the Photo-catalyzed Production of Hydrogen and Electricity Rosemary Khuu Le University of Tennessee - Knoxville, [email protected] Follow this and additional works at: https://trace.tennessee.edu/utk_graddiss Part of the Biochemical and Biomolecular Engineering Commons Recommended Citation Le, Rosemary Khuu, "Photosystem I-Based Applications for the Photo-catalyzed Production of Hydrogen and Electricity. " PhD diss., University of Tennessee, 2014. https://trace.tennessee.edu/utk_graddiss/3146 This Dissertation is brought to you for free and open access by the Graduate School at TRACE: Tennessee Research and Creative Exchange. It has been accepted for inclusion in Doctoral Dissertations by an authorized administrator of TRACE: Tennessee Research and Creative Exchange. For more information, please contact [email protected]. To the Graduate Council: I am submitting herewith a dissertation written by Rosemary Khuu Le entitled "Photosystem I- Based Applications for the Photo-catalyzed Production of Hydrogen and Electricity." I have examined the final electronic copy of this dissertation for form and content and recommend that it be accepted in partial fulfillment of the equirr ements for the degree of Doctor of Philosophy, with a major in Chemical Engineering. Paul D. Frymier, Major Professor We have read this dissertation and recommend its acceptance: Eric T. Boder, Barry D. Bruce, Hugh M. O'Neill Accepted for the Council: Carolyn R. Hodges Vice Provost and Dean of the Graduate School (Original signatures are on file with official studentecor r ds.) Photosystem I-Based Applications for the Photo-catalyzed Production of Hydrogen and Electricity A Dissertation Presented for the Doctor of Philosophy Degree The University of Tennessee, Knoxville Rosemary Khuu Le December 2014 Copyright © 2014 by Rosemary Khuu Le All rights reserved.
    [Show full text]
  • Can Ferredoxin and Ferredoxin NADP(H) Oxidoreductase Determine the Fate of Photosynthetic Electrons?
    Send Orders for Reprints to [email protected] Current Protein and Peptide Science, 2014, 15, 385-393 385 The End of the Line: Can Ferredoxin and Ferredoxin NADP(H) Oxidoreductase Determine the Fate of Photosynthetic Electrons? Tatjana Goss and Guy Hanke* Department of Plant Physiology, Faculty of Biology and Chemistry, University of Osnabrück,11 Barbara Strasse, Osnabrueck, DE-49076, Germany Abstract: At the end of the linear photosynthetic electron transfer (PET) chain, the small soluble protein ferredoxin (Fd) transfers electrons to Fd:NADP(H) oxidoreductase (FNR), which can then reduce NADP+ to support C assimilation. In addition to this linear electron flow (LEF), Fd is also thought to mediate electron flow back to the membrane complexes by different cyclic electron flow (CEF) pathways: either antimycin A sensitive, NAD(P)H complex dependent, or through FNR located at the cytochrome b6f complex. Both Fd and FNR are present in higher plant genomes as multiple gene cop- ies, and it is now known that specific Fd iso-proteins can promote CEF. In addition, FNR iso-proteins vary in their ability to dynamically interact with thylakoid membrane complexes, and it has been suggested that this may also play a role in CEF. We will highlight work on the different Fd-isoproteins and FNR-membrane association found in the bundle sheath (BSC) and mesophyll (MC) cell chloroplasts of the C4 plant maize. These two cell types perform predominantly CEF and LEF, and the properties and activities of Fd and FNR in the BSC and MC are therefore specialized for CEF and LEF re- spectively.
    [Show full text]
  • (CP) Gene of Papaya Ri
    Results and Discussion 4. RESULTS AND DISCUSSION 4.1 Genetic diversity analysis of coat protein (CP) gene of Papaya ringspot virus-P (PRSV-P) isolates from multiple locations of Western India Results – 4.1.1 Sequence analysis In this study, fourteen CP gene sequences of PRSV-P originating from multiple locations of Western Indian States, Gujarat and Maharashtra (Fig. 3.1), have been analyzed and compared with 46 other CP sequences from different geographic locations of America (8), Australia (1), Asia (13) and India (24) (Table 4.1; Fig. 4.1). The CP length of the present isolates varies from 855 to 861 nucleotides encoding 285 to 287 amino acids. Fig. 4.1: Amplification of PRSV-P coat protein (CP) gene from 14 isolates of Western India. From left to right lanes:1: Ladder (1Kb), 2: IN-GU-JN, 3: IN-GU-SU, 4: IN-GU-DS, 5: IN-GU-RM, 6: IN-GU-VL, 7: IN-MH-PN, 8: IN-MH-KO, 9: IN-MH-PL, 10: IN-MH-SN, 11: IN-MH-JL, 12: IN-MH-AM, 13: IN-MH-AM, 14: IN-MH-AK, 15: IN-MH-NS,16: Negative control. Red arrow indicates amplicon of Coat protein (CP) gene. Table 4.1: Sources of coat protein (CP) gene sequences of PRSV-P isolates from India and other countries used in this study. Country Name of Length GenBank Origin¥ Reference isolates* (nts) Acc No IN-GU-JN GU-Jamnagar 861 MG977140 This study IN-GU-SU GU-Surat 855 MG977142 This study IN-GU-DS GU-Desalpur 855 MG977139 This study India IN-GU-RM GU-Ratlam 858 MG977141 This study IN-GU-VL GU-Valsad 855 MG977143 This study IN-MH-PU MH-Pune 861 MH311882 This study Page | 36 Results and Discussion IN-MH-PN MH-Pune
    [Show full text]
  • Itraq-Based Proteome Profiling Revealed the Role of Phytochrome A
    www.nature.com/scientificreports OPEN iTRAQ‑based proteome profling revealed the role of Phytochrome A in regulating primary metabolism in tomato seedling Sherinmol Thomas1, Rakesh Kumar2,3, Kapil Sharma2, Abhilash Barpanda1, Yellamaraju Sreelakshmi2, Rameshwar Sharma2 & Sanjeeva Srivastava1* In plants, during growth and development, photoreceptors monitor fuctuations in their environment and adjust their metabolism as a strategy of surveillance. Phytochromes (Phys) play an essential role in plant growth and development, from germination to fruit development. FR‑light (FR) insensitive mutant (fri) carries a recessive mutation in Phytochrome A and is characterized by the failure to de‑etiolate in continuous FR. Here we used iTRAQ‑based quantitative proteomics along with metabolomics to unravel the role of Phytochrome A in regulating central metabolism in tomato seedlings grown under FR. Our results indicate that Phytochrome A has a predominant role in FR‑mediated establishment of the mature seedling proteome. Further, we observed temporal regulation in the expression of several of the late response proteins associated with central metabolism. The proteomics investigations identifed a decreased abundance of enzymes involved in photosynthesis and carbon fxation in the mutant. Profound accumulation of storage proteins in the mutant ascertained the possible conversion of sugars into storage material instead of being used or the retention of an earlier profle associated with the mature embryo. The enhanced accumulation of organic sugars in the seedlings indicates the absence of photomorphogenesis in the mutant. Plant development is intimately bound to the external light environment. Light drives photosynthetic carbon fxa- tion and activates a set of signal-transducing photoreceptors that regulate plant growth and development.
    [Show full text]
  • CV – Professor Toshiki ENOMOTO
    CV – Professor Toshiki ENOMOTO Name: Toshiki ENOMOTO, Ph.D. Date of Birth: May 14th 1958 Nationality: Japanese Present Position: Professor of Department of Food Science (Food Chemistry Laboratory), Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University Office Address 1-308 Suematsu, Nonoichi, Ishikawa 921-8836, Japan Tel and Fax: +81-76-227-7452 E-mail: [email protected] Educational Background: 1983 Received BS. From Dept of Animal Science, Obihiro University of Agriculture and Veterinary Medicine 1985 Received MS. From Graduate School of Agricultural Science, Osaka Prefecture University 1988 Received PhD. From Graduate School of Agricultural Science, Osaka Prefecture University Research Field: Food Chemistry and Food Function Research Themes in 2019 1. Identification of oligosaccharides in honey 2. The volatile compounds of Kaga boucya (roasted stem tea) 3. Chemical composition and microflora of Asian fermented fish products 4. Anti-influenza virus activity of agricultural, forestry and fishery products 5. Chemical composition and microflora of kefir 6. Elucidation of detoxification mechanism of tetrodotoxin from puffer fish ovaries picked in rice bran Professional Carrier: 1988 Assistant Professor, Division of Life and Health Sciences, 1 Joetsu University of Education 1993 Associate Professor, Department of Food Science, Ishikawa Agricultural College 1994-1995 Visiting Scientist Supported by Ministry of Education, Culture, Sports, Science and Technology-Japan, School of Biological Sciences, University
    [Show full text]
  • Supplementary Material Title Comparative Proteomic Analysis Of
    Supplementary Material Title Comparative proteomic analysis of wild-type Physcomitrella patens and an OPDA-deficient Physcomitrella patens mutant with disrupted PpAOS1 and PpAOS2 genes after wounding Authors Weifeng Luoa, Setsuko Komatsub, Tatsuya Abea, Hideyuki Matsuuraa, and Kosaku Takahashia,c* Affiliations aResearch Faculty of Agriculture, Hokkaido University, Sapporo 606-8589, Japan bDepartment of Environmental and Food Sciences, Faculty of Environmental and Information Sciences, Fukui University of Technology, 3-6-1 Gakuen, Fukui 910-8505, Japan cDepartment of Nutritional Science, Faculty of Applied BioScience, Tokyo University of Agriculture, Tokyo 165-8502, Japan. *To whom correspondence should be addressed: Tel.: +81-3-5477-2679; E-mail: [email protected]. Supplementary Fig. S1. Fig. S1. Disruption of PpAOS1 and PpAOS2 genes in P. p a te ns . A, Genomic structures of PpAOS1 and PpAOS2 in the wild-type and targeted PpAOS1 and PpAOS2 knock-out mutants (A5, A19, and A22). The npt II and aph IV expression cassettes were inserted in A5, A19, and A22 strains. B, Genomic PCR data of wild-type, and A5, A19 and A22 strains. P. patens genomic DNA was isolated from protonemata by the CTAB method (Nishiyama et al., Ref. 32). PCR was performed in 50 µL of a reaction mixture containing 1 µL of genomic DNA solution, 1.5 µL of each primer (5 µM), 25 µL of KOD One PCR Master Mix (Toyobo, Japan), and 21 µL of Milli-Q water. PCR was conducted with the following conditions: 30 cycles of 98°C for 10 s, 58°C for 5 s, and 68°C for 15 s.
    [Show full text]
  • Chloroplast Metabolism and Photosynthesis Meeting Abstracts Faculté Des Sciences Unimail, Emile-Argand 11, Aula Louis-Guillaume (ALG, 2Nd Floor)
    Chloroplast Metabolism and Photosynthesis Meeting Abstracts Faculté des Sciences UniMail, Emile-Argand 11, Aula Louis-Guillaume (ALG, 2nd floor) Invited Speakers (IS) Selected Speakers (SS) 26 June 2017 Session 1 – Mechanism of photosynthesis Chair : Jean-David Rochaix IS-1: BREAKING THE DOGMA: PHOTOSYNTHESIS WITHOUT CAROTENES. Pengqi Xu, Vohla U. Chukhutsina, Gert Schansker, Ludwik W. Bielczynski, Yinghong Lu, Daniel Karcher, Ralph Bock and Roberta Croce Biophysics of Photosynthesis, Faculty of Science, VU University Amsterdam, The Netherlands and Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany. Contact: [email protected] Carotenoids play essential roles in plant photosynthesis: they stabilize the pigment-protein complexes, are active in harvesting sunlight and are especially involved in photoprotection via several different mechanisms. In plants, carotenoids are present as carotenes, mainly in the form of ȕ-carotene, associated with the core complexes of Photosystem II and I, and as xanthophylls, a variety of oxygenated derivates, which are bound to the light-harvesting complexes. While mutant plants lacking xanthophylls show photoautotrophic grow, no plants lacking carotenes have been reported so far, leading to the conclusion that carotenes are essential for the survival of the plant. We have studied an N.tobacco plant modified to produce astaxanthin, a ketocarotenoid. We show that these plants do not contain any carotenes and that astaxanthin is the only xanthophyll present. Despite this, they grow photoautotrophically, showing that carotenes are not essential for photosynthesis. The effect of the absence of ȕ- carotene on the components of the photosynthetic apparatus is discussed. IS-2: PHOTOPROTECTION IN CYANOBACTERIA: THE ORANGE CAROTENOID PROTEIN AND THE OCP RELATED PROTEINS.
    [Show full text]