Charlotte E. Hacker, Ph.D. * [email protected]

Charlotte E. Hacker, Ph.D. * Hackerc@Duq.Edu

Updated Jul. 2021

Education • PhD Biology. 2021. Advisor: Dr. Jan E. Janecka. Duquesne University. Pittsburgh, PA 15282. 2020 Bayer Fellow | 2019 Fulbright Scholar | 2017 National Science Foundation EAPSI Fellow o PhD Research: Understanding snow leopard (Panthera uncia) status, diet, and human dimensions using noninvasive genetic approaches o Candidacy - Written Qualifying Exam: passed May 2017 | Oral Qualifying Exam: passed April 2018

• MS Biology. 2015. Advisor: Dr. Bruce A. Schulte. Western Kentucky University. Bowling Green, KY 42101. o MS Thesis Research: Examination of space and food enrichment on African elephants (Loxodonta africana) at the San Diego Zoo Safari Park

• BS Biology. International Studies minor. 2012. Wilkes University. Wilkes-Barre, PA 18702. o Capstone Research: The correlation of acorn masts and subsequent small mammal populations

Publications (Peer Reviewed) Journal Articles + indicates corresponding author | * indicates co-first authorship Accepted or Published: 1. Hacker CE, Hoenig BD, Wu L, Cong W, Yu J, Dai Y, Li Y, Li J, Xue Y, Zhang Y, Ji Y, Cao H, Li D, Zhang Y, Janecka J. Use of DNA metabarcoding of bird pellets in understanding raptor diet on the Qinghai-Tibetan Plateau Avi Res Accepted. 2. Xue Y, Li J, Zhang Y, Li D, Yuan L, Cheng Y, Liu S, Hacker CE. 2021. Assessing the vulnerability and adaptation strategies of wild camel to climate change in the Kumtag Desert of China. Global Ecol and Conserv e01725. 3. Khan TU, Mannan A, Hacker CE, Ahmad S, Chen M, Wanghe K, Zhang C, Nizami M, Luan X. 2021. Use of GIS and remote sensing data to understand impacts of land use/land cover chances (LULCC) on snow leopard (Panthera uncia) habitat in Pakistan. Sustainability DOI:10.3390/su13073590. 4. Dai Y, Peng G, Wen C, Zahoor B, Ma X, Hacker CE+, Xue Y. 2021. Climate and land use changes shift the distribution and dispersal of two umbrella species in the Hindu Kush Himalayan region. Sci Total Environ DOI:10.1016/j.scitotenv.2021.146207. 5. Hacker CE, Jevit M, Hussain S, Muhammad G, Munkhtsog B, Munkhtsog B, Zhang Y, Li D, Liu Y, Farrington J, Balbakova F, Alamanov A, Kurmanaliev O, Buyanaa C, Bavandonoj G, Ochirjay M, Liang X, Xiao X, Weckworth B, Jackson R, Janecka J. 2021. Regional comparison of snow leopard diet using DNA metabarcoding. Biodiv and Conserv DOI: 10.1007/210531-021-02118-6. 6. Dai Y, Hacker CE, Cao Y, Cao H, Xue Y, Ma X, Liu H, Zahoor, B, Zhang Y. 2021. Implementing a comprehensive approach to study the causes of human-bear (Ursus arctos pruinosus) conflicts in the Sanjiangyuan region, China. Sci Total Environ DOI: 10.1016/j.scitotenv.2021.145012. 7. Li J, Xue Y, Zhang Y, Dong W, Shan G, Sun R, Hacker CE, Wu B, Li D. 2020. Spatial and temporal activity patterns of golden takin (Budorcas taxicolor bedfordi) recorded by camera trapping. Peer J DOI: 10.7717/peerj.10353. 8. Hacker CE, Janecka JE, Jackson RM, Li D, Zhang Y. 2020. Pastoralism partnerships: recognizing the value of herder involvement in China’s snow leopard conservation efforts. Sustainability DOI: 10/3390/su12166491. 9. Hacker CE, Dai Y, Yifan C, Zhang Y, Zhang Y, Miller LJ, Janecka JE. 2020. Determinants of herder attitudes towards the threatened snow leopard (Panthera uncia) in Yushu Prefecture, China. Oryx DOI: 10.1017/S0030605319001315. 10. Dai Y, Hacker CE, Zhang Y, Li Y, Li J, Xue Y, Li D. 2020. Conflicts of human with the Tibetan brown bear (Ursus arctos pruinosus) in the Sanjiangyuan region, China. Global Ecol and Evol DOI: 10.1016/j.gecco.2020.e01039. 11. Ahmad S, Ullah Khan T, Hacker CE, Yang L, Nabi G, Ullah S, Wanghe K, Shah S, Chen M, Luan X. 2020. Critical assessment of Asiatic ibex for sustainable harvesting in northern areas of Pakistan. Global Ecol and Cons DOI: 10.1016/e00907.

Charlotte Hacker Curriculum Vitae Updated Jul. 2021 12. Dai Y, Xue Y, Hacker CE, Li J, Li W, Zhang Y, Li D. 2020. Human-carnivore conflicts and mitigation options in Qinghai Province, China. J for Nat Conserv 53:125776. 13. Dai Y, Hacker CE, Zhang Y, Li W, Li J, Zhang Y, Bona G, Liu H, Li Y, Xue Y, Li D. 2019. Identifying the risk regions of house break-ins caused by Tibetan brown bears (Ursus arctos pruinosus) in the Sanjiangyuan region, China. Ecol and Evol DOI: 10.1992/ece3.5835. 14. Dai Y, Hacker CE, Zhang Y, Liu H, Yu Z, Li W, Zhang J, Ji Y, Xue Y, Diqiang L. 2019. Identifying climate refugia and its potential impact on Tibetan brown bear (Ursus arctos pruinosus) in Sanjiangyuan National Park, China. Ecol and Evol DOI: 10.1002/ece3.5780. 15. Zhang Y, Hacker CE, Zhang Y, Xue Y, Wu L, Dai Y, Ping L, X, Janecka JE, Li D. 2019. The genetic structure of snow leopard populations in Sanjiangyuan and Qilianshan National Parks. Acta Theriologica Sinica 39: 442-449. 16. Hacker CE+, Miller LJ, Schulte BA. 2018. Examination of space and food enrichment on the African elephants (Loxodonta africana) at the San Diego Zoo Safari Park. Anim Welf 27: 55-65. 17. Janecka JE, Zhang Y, Li D, Munkhtsog B, Bayaraa M, Galsandorj N, Wangchuk TR, Karmachary D, Li J, Lu Z, Uulu KZ, Gaur A, Kumar S, Kumar K, Hussain S, Muhammad G, Jevit M, Hacker CE, Burger P, Wultsch C, Janecka MJ, Helgen K, Murphy WJ, Jackson R. 2017. Range-wide snow leopard phylogeography supports three subspecies. J of Heredity 108: 597-607. *highlighted in Newsweek and ScienceDaily *received journal cover 18. Hacker CE+, Miller LJ. 2016. Zoo visitor perceptions, attitudes and conservation intent after viewing African elephants at the San Diego Zoo Safari Park. Zoo Biol 9999: 1-7. 19. Miller LJ, Chase M, Hacker CE. 2016. A comparison of walking rates between zoo and wild elephants. J of Appl Anim Welf Sci 19: 271-279. 20. Hacker CE+, Horback KM, Miller LJ. 2014. GPS technology as a proxy tool for determining relationships in social animals: An example with African elephants. Appl Anim Behav Sci 163: 175-182. *highlighted in Connect Magazine

In Review or Preparation: 1. Hacker CE, Cong W, Xue Y, Li J, Zhang Y, Wu L, Li D, Janecka JE, Zhang Y. Dietary diversity and niche-partitioning of predator species across the Qinghai-Tibetan Plateau of China using DNA metabarcoding Biol Cons In Review. 2. Hacker CE, Li Y, Li J, Li D, Janecka J, Zhang Y, Jackson R. The functional and perceived effectiveness of Foxlights at preventing livestock loss on the Qinghai-Tibetan Plateau Hum Dimens of Wildl In Review. 3. Ullah Khan T, Hacker CE, Mannan A, Saddique MA, Luan X. Letter to the editor: conservation strategies are needed to minimize chances for wildlife-vehicle collisions along the China Pakistan Economic Corridor (CPEC) Biodiv and Cons In Review. 4. Dai Y, Peng G, Zahoor B, Hacker CE. Construction of ecological security patterns in Chongqing Municipality, Southwest China. Sci Total Environ In Review. 5. Li J, Xue Y, Zhang Y, Li Y, Wei C, Zhang Y, Li D, Hacker CE, Wu B. Projected impacts of climate change on snow leopard habitat in Qinghai Province, China In Prep. 6. Hacker CE, Atzeni L, Munkhtsog B, Munkhtsog B, Galsandorj N, Zhang Y, Liu Y, Buyanaa C, Bayandonoi G, Ochirjav M, Farrington JD, Jevit M, Zhang Y, Wu L, Cong W, Li D, Gavette C, Jackson R, Janecka J. Genetic diversity, structure, and connectivity of snow leopards (Panthera uncia) across Mongolia and northwestern China In Prep. 7. Hacker CE, Cong W, Dai Y, Xue Y, Bao R, Li D, Janecka JE, Zhang Y. Seasonal differences in dietary composition of a predator guild in China In Prep.

Book Chapters Accepted or Published: 1. Janecka JE, Hacker CE, Broderick J, Pulugulla S, Auron P, Ringling M, Nelson B, Munkhtsog B, Hussain S, Davis B, Jackson R. 2020. Noninvasive genetics and genomics sheds light on the status, phylogeography, and evolution of elusive carnivores: The case of the snow leopard. In: Conservation Genomics of Mammals – Integrative Research Using Novel Approaches. J Ortega and JE Maldonado, Eds. Springer, Switzerland. Pp. 83-120.

In Prep: 1. Hacker CE*, Cancellare I*, Caragiulo A, Janecka JE, Weckworth B. Conservation genetics of snow leopards. In: Snow Leopards – Biodiversity of the World: Conservation from Genes to Landscapes. 2nd Edition. T McCarthy and Mallon D, Eds. Elsevier. 2 Charlotte Hacker Curriculum Vitae Updated Jul. 2021 Grants & Fellowships • May 2020 – Pres. | Duquesne University, Bayer Fellowship | $13,500 “Understanding snow leopard (Panthera uncia) status and diet using noninvasive genetics” • Jul. 2019 – Pres. | Panthera, Sabin Snow Leopard Grant Program | $20,000 “Snow leopard (Panthera uncia) prey use and dependence on livestock in Qinghai, China and implications for conservation” • Apr. 2019 – Pres. |Chicago Zoological Society Board of Trade Endangered Species Fund | $5,000 “Determination of snow leopard (Panthera uncia) prey use and livestock dependence using noninvasive genetic analyses” • Mar. 2019 – Pres.| Fulbright Student Scholar China | $22,825 “Temporal changes in prey use and livestock dependence in snow leopard” • Jul. 2018 | The Wildlife Society, Student Travel Grant | $400 “Regional comparison of snow leopard dependence on livestock using molecular diet analysis” • Jun. 2018 – Jun. 2019 | Panthera, Sabin Snow Leopard Grant Program | $5,500 “Snow leopard (Panthera uncia) prey use and dependence on livestock in Qinghai, China and its implications for conservation” • May 2018 – Sept. 2019 | Cleveland Metroparks Zoo, Asia Seeds Grant Program | $3,500 “Noninvasive genetic survey of snow leopards (Panthera uncia) in an unsampled region of China for resolution of subspecies boundaries” • Jun. – Aug. 2017 | National Science Foundation, East Asia and Pacific Summer Institute Fellowship | $5,400 “Noninvasive population survey of snow leopards in Qinghai Province, China” • Aug. 2016; May 2017; May 2018 | Duquesne University Graduate Student Assistantship | $24,700; $25,200; $26,000 • Jan. 2013; Aug. 2014 | Western Kentucky University Graduate Student Assistantship | $8,206; $14,550 • Jul. 2013 – Aug. 2014 | San Diego Zoo Institute of Conservation Research Heller Fellowship | $35,000 • Apr. 2013 | Western Kentucky University Graduate Student Grant | $1,500 “Effects of quality versus quantity on African elephant (Loxodonta africana) welfare”

Awards • Feb. 2020 | Wilkes University, “40 Under 40” alumni • Dec. 2019 | Reach The World, Fall 2019 Traveler of the Cycle • Mar. 2019 | Duquesne University, Graduate Symposium Excellence in Graduate Research Award | $300 • Aug. 2018 | Duquesne University, Top BioBlitz Presentation | $250 • Jul. 2016 | Toledo Zoo Above and Beyond Award • Apr. 2012 | Wilkes University, Global Scholar and Citizen Award

Research Experience Nov. 2019 – Pres. | Understanding transboundary snow leopard (Panthera uncia) populations with PhD student Tauheed Ullah Khan, supervisor Dr. Xiaofeng Luan (Beijing Forestry University (BFU)), and host advisor Dr. Yuguang Zhang (Chinese Academy of Forestry (CAF)) • Studies across country borders have been lacking. This work studies populations from Pakistan into China. • Experience gained: geography of Pakistan, construction of memorandums of understanding between institutions

Sept. 2019 – Jul. 2020. | Temporal changes in carnivore prey use with PhD advisor Dr. Jan Janecka (Duquesne University (Duq U)), PhD students Yunchuan Dai, Li Ye, and Cong Wei, postdoctoral researcher Dr. Li Jia, and host advisors Dr. Yuguang Zhang and Dr. Li Diqiang (CAF), and Gouli Nature Reserve director Rou Bao. • Dietary temporal changes have conservation implications for human-wildlife conflict and climate change adaptations. This study used DNA metabarcoding of scat collected seasonally. A manuscript is in prep. • Experience gained: permit acquisition, leading field surveys, safety procedures in remote environments

Aug. 2019 – Mar. 2020 | Effectiveness of Foxlights in preventing livestock loss with PhD advisor Dr. Jan Janecka (Duq U), PhD student Li Ye, postdoctoral researcher Dr. Li Jia, host advisor Dr. Yuguang Zhang (CAF), and collaborator Dr. Rodney Jackson (Snow leopard Conservancy) • Livestock depredation encourages retaliatory killings and negative attitudes. Scientific testing of mitigation methods is needed to determine efficacy in preventing loss. A manuscript is in review. • Experience gained: national park permit acquisition, Institutional Review Board (IRB) document preparation

3 Charlotte Hacker Curriculum Vitae Updated Jul. 2021 Jun. 2019 – Jan. 2021 | Assessing the vulnerability of wild Bactrian camel (Camelus ferus) to future climate change with researchers Dr. Xue Yadong and Dr. Li Jia, Professor Dr. Yu Zhang, host advisor Dr. Diqiang Li (CAF), and national park personnel Lei Yuan (Xingjian Lop Nur Wild Came National Nature Reserve) • Efforts to preserve the wild Bactrian population in Mongolia and China have been successful. However, how the species may respond to climate change is largely unknown. This study used MaxEnt modeling to determine current and future suitable habitat under a mild climate change scenario. Study manuscript published in Global Ecology and Conservation in 2021. • Experience gained: National park management, MaxEnt modeling, manuscript editing.

Jun. – Aug. 2019 | Identifying climate refugia and its impact on Tibetan brown bear (Ursus arctos pruinosus) and Himalayan brown bear (U. a. isabellinus) with PhD student Yuchuan Dai and Li Ye, professor Dr. Zhang Yu, and host advisors Dr. Yuguang Zhang and Dr. Li Diqiang (CAF). • Brown bears are at risk for distribution shifts due to climate change. Modeling was used to identify climate refugia and to determine dispersal paths used in both current and future climate scenarios. Study manuscript published in Ecology & Evolution in 2019. A second manuscript is in prep. • Experience gained: creation of species distribution models, manuscript editing

May 2019 – Aug. 2019 | Functional impacts of gene variants in the hypoxia pathway and its role in predicting snow leopard adaptability to climate change with PhD advisor Dr. Jan Janecka, collaborator Dr. Philip Auron, and PhD student David Macar (Duq U), and undergraduate students Julia Plasynski (Washington & Jefferson College) and Jaime Calcagno (Chowan University). • Snow leopards are adapted for high altitudes with low oxygen and at risk for the negative impacts of climate change. Little is known of the functional impacts of genes important to the hypoxia pathway. Blood cells from snow leopard, domestic cat (Felis catus), lion (P. leo), and tiger (P. tigris) were cultured in hypoxic and normoxic environments, expression levels of hypoxia related genes were then compared. • Experience gained: RNA extractions, cell culture, qPCR.

Mar. 2019 – Jun. 2020 | Assessment of Asiatic ibex (Capra ibex sibirica) for harvest in northern Pakistan with PhD students Shahid Ahmad and Tauheed Ullah Khan, and advisor Dr. Xiaofeng Luan (BFU). • Trophy hunting of the Asiatic ibex is an important income source for local communities. Reliable estimates of Asiatic ibex are crucial for hunting to meet the needs of the community and conservation initiatives. This study used the double-observer method and camera trapping to estimate Asiatic ibex in hunting areas of northern Pakistan. A manuscript of study findings was published in Global Ecology and Conservation in June 2020. • Experience gained: geography of Pakistan, ungulate ecology, double-observer method, manuscript editing.

Jul. 2018 | Assessment of the current status of human-carnivore conflict (HCC) in Qinghai province, China with PhD student Dai Yunchuan, and host advisors Dr. Yuguang Zhang and Dr. Li Diqiang (CAF). • Knowledge of the status and patterns of HCC is necessary for designing and implementing conservation action plans that promote peaceful coexistence between humans and wildlife. Reported incidents of HCC over a four- year period we analyzed to determine the current status of conflict and to propose solutions aimed at reducing their occurrence rate. Study manuscript published in Journal for Nature Conservation in 2020. • Experience gained: familiarity with political and topographical maps of Qinghai province, analysis of demographic data, mechanisms of HCC mitigation.

Jul. 2018 | Herder attitudes towards the at-risk snow leopard and Tibetan brown bears in Yushu Prefecture, China with PhD advisor Dr. Jan Janecka (Duq U), PhD student Yunchuan Dai, PhD student Zhang Yu, Masters student Cheng Yifan, host advisor Dr. Yuguang Zhang (CAF), and collaborator Dr. Lance Miller (Chicago Zoological Society). • Successful implementation of laws and policies protecting wildlife hinge on local resident attitudes towards protected species. Herders were interviewed to assess their attitudes and perceptions towards two large predators within the context of livestock predation. Study manuscript published in Oryx (snow leopards) in 2019. • Experience gained: Basic Mandarin Chinese and Tibetan, livestock economy, human wildlife coexistence and conflict mitigation practices, national park management.

4 Charlotte Hacker Curriculum Vitae Updated Jul. 2021 Jun. 2018 – Jul. 2019 | SNP panel design for comparative genomics across proposed snow leopard subspecies to examine high-altitude adaptations with PhD advisor Dr. Jan Janecka, Masters student McKenna Rigling, PhD student Jennifer Broderick (Duq U), high school student Daniella Naumov (Taylor Alderdice High School), and undergraduate student Julia Plasynksi (Washington & Jefferson College). • Snow leopard range spans from 2,000m to 5,500m. High altitude adaptations involving hypoxia may vary between populations. Knowledge of this variation can help predict how snow leopards may respond to range shifts as a result of climate change. • Experience gained: undergraduate mentoring, SNP analysis.

Dec. 2017 – Jan. 2021 | Regional differences in snow leopard diet and its conservation implications with PhD advisor Dr. Jan Janecka (Duq U), collaborator Dr. Bariushaa Munkhtsog (Mongolian Academy of Sciences), collaborator Dr. Shafqat Hussein (Trinity College), collaborator Dr. Byron Weckworth (Panthera), and collaborator Ghulam Mohammad (Baltistan Wildlife Conservation and Development Organization) • Snow leopard range is approximately 2 million km2. Prey availability and dietary preferences vary throughout, creating unique differences in conservation challenges and their application. This study sought to examine snow leopard diet using DNA metabarcoding to investigate differences in snow leopard diet in six regions of snow leopard range. A study manuscript of this work was published in Biodiversity and Conservation in 2021. • Experience gained: ArcGIS, navigating IUCN database, managing multiple co-authors.

Jul. 2017 – Aug. 2019 | Mapping risk areas for home break-ins by Tibetan brown bears (U. arctos pruinosus) in Zhiduo County, Qinghai Province, China with PhD student Dai Yunchuan, research assistant Dr. Xue Yadong, postdoctoral researcher Dr. Li Jia, and host advisors Dr. Yuguang Zhang and Dr. Diqiang Li (CAF). • Targeted efforts are needed where risk of home break-ins by bears is high. MaxEnt was used to identify high-risk regions and Circuit theory used to determine bear diffusion paths. Environmental variables were linked to risk and conservation recommendations provided. Study manuscript published in Ecology & Evolution in 2019. • Experience gained: Tibetan brown bear ecology, manuscript editing, use of ArcGIS.

Sept. 2017 – Jan. 2019 | Optimization of molecular diet analysis of snow leopard with PhD advisor Dr. Jan Janecka (Duq U) and PhD student Matthew Jevit (Texas A&M University) • Molecular diet analysis can determine how snow leopards exploit resources, including livestock. Previous DNA barcoding methods have been unable to discern closely related wild and domestic species (ie. argali vs. sheep) using a universal primer. This study sought to use another mtDNA sequence suitable for differentiation. • Experience gained: Illumina sequencing, DNA library prep, navigating NCBI and BOLD database systems, use of MEGA7 and CLC Genomics Workbench.

Jul. 2017 – Sept. 2019 | Spatial and temporal activity patterns of golden takin (Budorcas taxicolor bedfordi) with postdoctoral researcher Li Jia (CAF) • Use of time-stamped camera trapping has the ability to discern the behavior of elusive animals in remote environments. This study aimed to use this remote sensing technique to determine the daily and seasonal activity patterns of an at-risk ungulate in China. A study manuscript was published in PeerJ in 2020. • Experience gained: English manuscript editing, behavioral identification from camera trap photos.

Jun. 2017 – Aug. 2018 | Noninvasive genetic analysis of snow leopards (P. uncia) in China with PhD advisor Dr. Jan Janecka (Duq U), PhD student Zhang Yu, PhD student Yunchuan Dai, postdoctoral researcher Xue Yadong, host advisors Dr. Diqiang Li and Dr. Yuguang Zhang (CAF), Nature Reserve Manger Wu Liji (Yanchiwan National Nature Reserve), and collaborator Xierannima (Suojia Township). • China has the largest population of snow leopards. Genetic assessments are vital for making informed conservation management decisions. Study manuscript was published in Acta Theriologica Sinica in 2019. • Experience gained: DNA extraction, China wildlife and conservation policies, hiking difficult terrain, CITES and USDA regulations, abundance estimates using capture-mark-recapture, publication in Mandarin Chinese.

5 Charlotte Hacker Curriculum Vitae Updated Jul. 2021 Nov. – Dec. 2017 | Using range-wide genomics to combat illegal trafficking of snow leopard parts with PhD advisor Dr. Jan Janecka (Duq U) and collaborator Dr. Pamela Burger (University of Veterinary Medicine, Vienna) • Threats to snow leopards include poaching and retaliatory killings post-livestock predation. Confiscated pelts from Mongolia were genotyped at nine loci and assigned to region using a range-wide genomic dataset. • Experience gained: Wildlife trafficking laws and regulations, use of GenAlEx and GeneClass.

Jan. 2017 – Mar. 2017 | Using eDNA for monitoring big-river fish communities: primer development for ray-finned fish with supervisor Dr. Brady Porter (Duq U) • Environmental DNA (eDNA) may avoid issues associated with traditional fish sampling methods. This study aimed to improve surveying capacity of eDNA by testing generalized fish primer sets optimal for ray-finned fish. • Experience gained: primer design, fish taxonomy, lab management, supply ordering.

Aug. 2016 – Pres. | Landscape genetics of snow leopards (P. uncia) in Mongolia and northwestern China with PhD advisor Dr. Jan Janecka (Duq U), collaborator Dr. Bariushaa Munkhtsog (Mongolian Academy of Sciences), host advisors Dr. Diqiang Li and Dr. Yuguang Zhang (CAF) • This study used genetic profiles from snow leopards in Mongolia and China to determine population parameters, variables important to habitat suitability, and used genetic distance as an inference for landscape connectivity. • Experience gained: PCR, gel electrophoresis, sample prep for genotyping, allele calling, use of GeneMarker, habitat suitability modeling, use of Circuitscape.

Aug. 2015 – Feb. 2016| Preliminary observations of the Aruban whiptail lizard (Cnemidophorus arubensis) with supervisor Beth Posta and herpetologist Andrew Odum (Toledo Zoo) • Little is known about Aruban whiptail lizard ecology and behavior. Behavioral observations were conducted via remote video cameras and a report outlining husbandry recommendations was submitted to zoo staff. • Experience gained: reptilian husbandry, international animal relocation, quarantine protocols.

Jun. 2015 – Feb. 2016 | African elephant (Loxodonta africana) food preference testing in relation to cognitive capabilities – phase 1 with supervisor Beth Posta (Toledo Zoo) • A female elephant was provided with a choice of paired foods to assess preference. Food items were then paired with a 2-D shape to examine the elephant’s capability of associated the shape with the food item. • Experience gained: animal psychology, research design troubleshooting, animal training.

Jun. 2015 – Feb. 2016| Effect of environment on indicators of American toad (Anaxyrus americanus) welfare with supervisor Beth Posta and herpetologist Andrew Odum (Toledo Zoo) • Toad location within five different coded areas of three environmentally different tanks along with body condition were recorded to assess space use and physical welfare. • Experience gained: amphibian husbandry, space use analysis, non-invasive data collection techniques.

Mar. – July 2014 | Examination of space and food enrichment on African elephant at the San Diego Zoo Safari Park (SDZSP) with MS advisor Dr. Bruce Schulte (Western Kentucky University) and supervisor Dr. Lance Miller (San Diego Zoo Institute for Conservation Research) • MS thesis research; examined effects of space and food on behavior, walking activity, and stress hormones on 13 African elephants at the SDZSP in Escondido, CA. Study manuscript published in Animal Welfare in 2017. • Experience gained: enrichment set up, working with zoo staff, operating heavy machinery, research design and implementation, statistical analysis using R, proposal and grant writing, hormone analyses using RIA and EIA.

Jul. – Jan. 2014 | Zoo visitor perceptions, attitudes and conservation intent after viewing African elephants at the San Diego Zoo Safari Park with supervisor Dr. Lance Miller (San Diego Zoo Institute for Conservation Research) • Two surveys assessing visitor attitudes or emotions and perceptions, intended conservation behaviors and demographics were given to elephant exhibit guests. Study manuscript published in Zoo Biology in 2016. • Experience gained: survey construction, coordinating and instructing volunteers, data entry, statistical analysis using SPSS, assembling a literature review and writing scientific manuscripts.

6 Charlotte Hacker Curriculum Vitae Updated Jul. 2021 Sept. – Dec. 2013 | GPS technology as a proxy tool for determining relationships in social animals: an example with African elephants with supervisor Dr. Lance Miller (San Diego Zoo Institute for Conservation Research) and postdoctoral researcher Kristina Horback (University of Pennsylvania) • Examined the use of GPS data to determine social relationships among elephants at the SDZSP. Study manuscript published in Applied Animal Behaviour Science in 2014. • Experience gained: data organization and sorting, statistical analysis using SPSS, writing scientific manuscripts and the submission process to a peer-reviewed academic journal.

Jul. – Nov. 2013 | A comparison of walking rates between wild and zoo African elephants with supervisor Dr. Lance Miller (San Diego Zoo Institute for Conservation Research) and researcher Dr. Michael Chase (Save the Elephants) • There is little coordination among wild and captive studies exploring walking rates; elephants at the SDZSP and in Botswana were collared with fixed intervals allowing for direct comparison between the two populations. Study manuscript was published in Journal of Applied Animal Welfare Science in 2016. • Experience gained: data examination, sorting and organization, GPS technology and literature review assembly.

Sept. 2011 – May 2012 | The correlation of acorn masts and subsequent small mammal populations with BS advisor Dr. Michael Steele (Wilkes University) • BS capstone research. This research focused on which species of oak had the greatest impact on small mammal populations as well as which species was most prone to weevil (Curculionoidea) infestation. • Experience gained: detailed data collection, small mammal trapping, statistical analysis, writing scientific manuscripts and presentation.

Sept. 2011 – Dec. 2012 | The contamination of gas drilling on birds with professors Dr. Jeffrey Stratford and Dr. William Biggers (Wilkes University) • Blood samples were taken from birds, an enzyme assay for Glutothione S-Transferase was used to see the damage of red blood cells caused by toxins with the use of a spectrometer. • Experience gained: The capture and handling of birds, taking blood samples, use of a spectrometer, creating a Glutothione S-Transferase assay.

Jan. – Apr. 2011 | Species of acorn on foraging ecology in squirrels with BS advisor Dr. Michael Steele (Wilkes University) • Small color-coded nails indicating red or white oak were implanted into acorns; acorns were then found using a metal detector, focal sampling or radio telemetry to see which had been buried and how far they were taken. • Experience gained: Use of metal detector and radio telemetry, classifying and counting acorn species.

Mar. 2011 | Mapping the Fear Landscape of gray squirrels with BS advisor Dr. Michael Steele (Wilkes University) • Food was placed in a gridded area in an urban park. Squirrel foraging behavior and location was documented to map a landscape of fear. • Experience gained: Behavioral observation, focal sampling, GPS use.

Nov. – Dec. 2010 | Observations of an African elephant at Imiri Safari Ranch, Wedza, Zimbabwe with owner Judy Travers (Imire Safari Ranch) • Assessed the behavior of a single 42-year-old elephant behaving as a matriarch to a herd of Cape buffalo. • Experience gained: diligent notetaking, working in close proximity to wild animals, communicating with individuals of different cultures.

7 Charlotte Hacker Curriculum Vitae Updated Jul. 2021 Conferences Attended & Research Presentations Underline: denotes presenter | ‡ invited talk Oral • Hacker CE, We C, Zhang Y, Wu, L, Weckworth B, Jackson R, Li D, Zhang Y, Janecka JE. 2021. Molecular dietary analysis of Tibetan wolf populations on the Qinghai-Tibetan Plateau of China. Northeast Fish and Wildlife Virtual Conference, Online. • ‡Hacker CE, Dai Y. 2021. Snow leopard and Tibetan brown bear conservation and research. Snow leopard Network, Online. https://www.youtube.com/watch?v=huOPyn4Mj_w • Hacker CE. 2020. DNA metabarcoding of snow leopard: application to other species and resolving carnivore conflict with humans. Graduate Student Seminar, Duquesne University, Online. • Hacker CE. 2020. The application of noninvasive genetics to studying the threatened snow leopard. Nature Conservation Foundation, Bengaluru, India. • Janecka JE, Hacker CE, Broderick J, Pulugulla S, Auron P, Ringling M, Nelson B, Munkhtsog B, Hussain S, Davis B, Zhang Y, Jackson R. 2019. Snow leopard genomics and function analyses reveals adaptation to high-altitude via regulatory changes in the hypoxia inducible pathway. American Fisheries & The Wildlife Society Joint Annual Conference, Reno, NV. • Hacker CE, Hussain S, Muhammad G, Janecka J. 2019. Panthera uncia (snow leopard) dependence on livestock in northern Pakistan and its implications with human-wildlife conflict mitigation. Pennsylvania Academy of Sciences, Allentown, PA. • Hacker CE, Jevit M, Janecka J, Hussain S, Muhammad G, Farrington J, Almanov A, Balbakova F, Munkhtsog B, Jackson RJ. 2018. Regional comparison of snow leopard dependence on livestock using molecular diet analysis. The Wildlife Society, Cleveland, OH. • Hacker CE. 2018. Exploring snow leopard diet using DNA metabarcoding and the implications for conservation. Duquesne University Work in Progress (WiP), Pittsburgh, PA • Hacker CE. 2018. Snow leopard genetics in China – another update. Duquesne University Annual Summer Retreat, Pymatuning State Park, PA § Placed 1st out of 8 talks for best bioblitz presentation. • Janecka JE, Hacker CE, Nelson B, Auron P, Pulugulla S, Murphy W, Davis B, Jackson B, Hussain S, Munkhtsog B, Muhammad G. Adaptation of snow leopards to high-altitude via regulatory changes in the hypoxia inducible pathway and potential ecological ramifications. ESA Annual Meeting, New Orleans, LA. • ‡Xue Y, Hacker CE, Zhang Y, Li D. 2018. Snow leopard genetics in Qilianshan National Park. With the Leopards International Research and Conservation Conference, Yushu, China • Hacker CE, Janecka J, Munkhtsog B, Munkhtsog B, Liu Y, Zhang Y, Li D, Ames C, Walker N, Jackson R, Xuchang L, Xiaoxing B, Farrington JD, Almanov A, Balbakova F, Weckworth B. 2018. Landscape connectivity of snow leopards in central Asia, Northeast Fish and Wildlife Conference, Burlington, VT • Hacker CE, Burger P, Janecka J. 2018. The application of phylogeography to combat snow leopard crime Duquesne University Graduate Student Recruitment Weekend, Pittsburgh, PA • Hacker CE. 2018. Snow leopard genetics in China – an update. Duquesne University Annual Summer Retreat, Pymatuning State Park, PA • ‡Hacker CE. 2017. Genetic variation and structure of snow leopards in China, Young Scientists’ Forum, Beijing, China • Hacker CE, Miller L, Schulte B. 2015. Examination of space and food enrichment on African elephant (Loxodonta africana) at the San Diego Zoo Safari Park. Western Kentucky University Student Research Conference, Bowling Green, KY • Hacker CE, Miller L, Schulte B. 2015. Examination of space and food enrichment on African elephant (Loxodonta africana) at the San Diego Zoo Safari Park. Kentucky Academy of Sciences, Lexington, KY § Placed 2nd out of 12 candidates for best research talk.

8 Charlotte Hacker Curriculum Vitae Updated Jul. 2021 Poster • Hacker CE, Zhang Y, Zhang Y, Janecka J. 2019. Population comparison of snow leopard (Panthera uncia) in China using a noninvasive genetics approach. Duquesne University Graduate Research Symposium, Pittsburgh, PA § Awarded Bayer School Excellence in Graduate Research award. • Rigling M, Naumov D, Hacker CE, Broderick J, Janecka J. Designing a SNP panel for comparative genomics in the snow leopard subspecies to examine high altitude adaptation. Duquesne University Undergraduate Summer Research Program Symposium, Pittsburgh, PA • Hacker CE, Gunderson G, Steele M. 2012. The correlation of acorn masts and subsequent small mammal populations. Wilkes University Biological Research Conference, Wilkes-Barre, PA

Work Experience English Editing for Scientific Writing Consultant | Aug. 2017 – Pres. | Self-Employed, Remote Responsible for English editing of scientific manuscripts for publication for non-native English speakers. Duties include substantive editing, structure editing, copy editing, proof-reading, assistance in peer-reviewed journal selection, assistance in writing cover letters for publishing cost waivers for under-represented scientists.

Graduate Teaching Assistant | Aug. 2016 – May 2019 | Duquesne University, Pittsburgh, PA Responsible for teaching two biology related lab or recitation sections. Duties include preparing lab lecture and teaching materials, lecturing, grading, and mentoring.

Enrichment Coordinator | Jun. 2015 – Aug. 2016 | Toledo Zoo, Toledo, OH Responsible for researching, coordinating, purchasing, implementing and evaluating enrichment provided for all animals and overseeing TZ interns in the Animal Behavior department. Duties include mentoring, safety adherence, communicating with staff, welfare assessments, data collection, entry, and analysis.

Graduate Teaching Assistant | Aug. 2014 – May 2015 | Western Kentucky University, Bowling Green, KY Responsible for teaching three Anatomy and Physiology lab sections. Duties included lecturing, grading, mentoring, clean up and maintaining Western Kentucky University’s code of conduct.

Research Technician | Jun. 2013 – Aug. 2014 | San Diego Zoo Global, San Diego, CA Responsible for completing various tasks, studies, and manuscripts dealing primarily with African elephants as well as adhering to the standards necessary to obtain a Masters’ thesis. Duties included data collection, data analysis, manuscript writing, grant writing, volunteer coordination, and other related lab tasks.

Graduate Teaching Assistant | Jan. 2013 – May 2013 | Western Kentucky University, Bowling Green, KY Responsible for teaching three introductory biology lab sections. Duties included lecturing, grading, creation of academic materials, and maintaining Western Kentucky University’s code of conduct.

Office Assistant | Jan. 2009 – May 2012 | Wilkes University, Wilkes-Barre, PA Responsible for greeting and directing students from around the world. Duties included answering telephones, filing, filling out paperwork, and general office work.

Teaching Experience Teaching Assistant General Biology | Spring 2017; Fall 2020 | Duquesne University, Pittsburgh, PA • Taught two General Biology lab sections (30 students each) for general science major freshman students. Life Processes | Fall 2016 | Duquesne University, Pittsburgh, PA • Taught two General Biology lab sections (30 students each) for nursing major freshman students. Genetics | Spring 2018; Spring 2019 | Duquesne University, Pittsburgh, PA • Taught two Genetics recitation sections (15 students each) to upper class biology major students. Advanced General Biology | Fall 2017; Fall 2018 | Duquesne University, Pittsburgh, PA • Taught two Advanced General Biology lab sections (25 students each) to biology major freshman students.

9 Charlotte Hacker Curriculum Vitae Updated Jul. 2021 (continued from previous page) Anatomy and Physiology | Fall 2014; Spring 2015 | Western Kentucky University, Bowling Green, KY • Taught three Anatomy and Physiology lab sections (30 students each) for nursing major junior students. General Biology | Spring 2013 | Western Kentucky University, Bowling Green, KY • Taught three Introductory Biology lab sections (30 students each) to biology major freshman students.

Guest Lecturer Methods in Population Genetics | Fall 2020 | SUNY College at Oneonta, Online, Instructor: Dr. Jeffrey Heilveil • Lectured on navigating graduate school post bachelor’s degree with an interest in population genetics. Science and Engineering of Animal Conservation | Spring 2020 | Georgia Tech, Online, Instructor: Andrew Schulz • Lectured on how DNA barcoding is used to study carnivore diet. Genetics | Spring 2018, 2019, 2021 | Duquesne University, Pittsburgh, PA, Instructor: Dr. Jan Janecka • Lectured on how textbook concepts are applied to wildlife conservation research. Mammalogy | Fall 2014 | Western Kentucky University, Bowling Green, KY, Instructor: Dr. Michael Stokes • Lectured on the evolution and taxonomic status of the order Sirenia to upper class biology students.

Mentoring & Training Experience Beijing Forestry University, Beijing, China | Aug. 2019 – Pres. • Mentor to two Pakistani PhD students using noninvasive methods to study snow leopards. Duties included training in DNA extraction, genotyping, sequencing, and writing scientific manuscripts in English. Chinese Academy of Forestry, Beijing, China | Jun. 2017 – Pres. • Mentor to five Chinese researchers using noninvasive methods to study snow leopards. Duties included training on scat collection, extraction, genotyping, NGS data analysis, and English manuscript writing. Duquesne University, Pittsburgh, PA | Aug. 2016 – Pres. • Mentor to two undergraduate students examining snow leopard high altitude adaptions at both genomic and functional levels during the university’s undergraduate summer research program. • Mentor to one undergraduate student assessing snow leopard population metrics in the Gobi-Altai of Mongolia. Duties included training in scat extractions, genotyping, and technical report writing. • Mentor to researcher from Pakistan studying snow leopard genetics. A one-week program was designed to include training on scat extractions and genotyping. Toledo Zoo, Toledo, OH | Jun. 2015 – Aug. 2016 • Mentor to 12 undergraduate seasonal interns studying animal behavior and welfare for species including western lowland gorillas, red pandas, and white rhinos. Duties included safety training, behavioral observation, report writing, and statistics. San Diego Zoo Global, San Diego, CA | Jun. 2013 – Aug. 2014 • Mentor to two undergraduate summer interns studying cheetah reproduction. Duties included training in behavioral monitoring, SSP and AZA record keeping protocols, and research dissemination.

Languages English - First Language | German – Intermediate | Mandarin Chinese - HSK2

10 Charlotte Hacker Curriculum Vitae Updated Jul. 2021 Professional Memberships & Organizations The Snow Leopard Network Full Member | Apr. 2020 – Pres. Snow Leopard Conservancy Research Associate | Jul. 2019 – Pres. https://snowleopardconservancy.org/associates/ Pennsylvania Academy of Sciences Member | Feb. 2019 – Pres. The Wildlife Society Member | Jun. 2018 – Pres. (TWS Molecular Ecology Working Group Member | Oct. 2018 – Pres) Association of Zoos and Aquariums Professional Affiliate | Jun. 2015 - 2016

Professional Development Geospatial Analysis in R | Northeast Fish & Wildlife Conference | Apr. 2021 Center for Teaching Excellence | Duquesne University o Encouraging and Supporting the Science Girl | Feb. 2021 o Developing a Teaching Portfolio | Oct. 2020 o Beyond the Lecture: Engaging Students with Zoom | Sept. 2020 o Writing a Teaching Philosophy Workshop | Sept. 2020 o Mobilizing Student Effort Through Learning | Oct. 2018 o Making First Lasting Impressions on Students | Oct. 2018 Next-Generation Sequencing MiSeq Training Workshop | Illumina | Dec. 2018 Responsible Conduct of Research | CITI o Social and Behavioral Research Course 1 Refresher | Jun. 2018 o Social and Behavioral Responsible Conduct of Research Course 1 | Feb. 2013 Lab Safety Training | Environmental Health and Safety, Duquesne University | May 2017; 2018; 2019 ESRI with Udemy | Going Places with Spatial Analysis, GIS online course | Jan. 2017 Blackboard Orientation Course | The Center for Educational Technology, Duquesne University | Aug. 2016 San Diego Zoo Global Academy | San Diego Zoo Global o Exhibit Design | Apr. 2014 o Animal Welfare | Mar. 2014 o Elephant Management Practices; Introduction to Enrichment | Feb. 2014 Basic Skills in College Teaching Program | Center for Excellence in Teaching, Western Kentucky University| Apr. 2013

University Service & Organizations Biology CIRCLE Boot Camp Mentor at Duquesne University | May 2019; May 2021 | Pittsburgh, PA Biology PhD applicant interview volunteer at Duquesne University | Feb. 2018; Feb. 2019; Feb. 2021 | Pittsburgh, PA Works in Progress Coordinator at Duquesne University | Aug. 2017 – Aug. 2018 | Pittsburgh, PA Women in Science at Duquesne University | Oct. 2016 – Pres. | Pittsburgh, PA Biology Department open house representative at Duquesne University | Oct. 2016; Oct. 2017 | Pittsburgh, PA Graduate Students of Bayer School (GSoBS) at Duquesne University | Aug. 2016 – Pres. | Pittsburgh, PA • Secretary | Aug. 2017 – May 2019 • Faculty Mentor Award Committee Chair | Mar. 2019 Graduate and Professional Student Council at Duquesne University | Aug. 2016 – Pres. | Pittsburgh, PA • Bayer School Biology Department Student Rep | Jun. 2018 – Jun. 2019 BSNES Association of Graduate Students (BAGS) at Duquesne University | Aug. 2016 – Pres. | Pittsburgh, PA Biograd Member at Western Kentucky University | Jan. 2013 – Dec. 2015| Bowling Green, KY Wilkes University Biology Club | Aug. 2008 – May 2012 | Wilkes-Barre, PA

11 Charlotte Hacker Curriculum Vitae Updated Jul. 2021 Science Communication The Women in Ecology and Evolution (W.E.E.) Podcast | May 2021 | Online https://www.theweepodcast.org/podcast/episode/4bd2a820/science-is-an-art The Fancy Scientist Podcast | Jan. 2021 | Online https://open.spotify.com/episode/7lpFDCtrpT24BsaMD80JFW?si=UgryAlMpRkOGIUHpCe-tDg Snow Leopard Network Training Initiative Module 5 Genetics Expert | Nov. 2020 | Online https://www.youtube.com/watch?v=CjJMeFnP1oY&list=PL8tq3jQmKIAqWOzKn489NNL548ClOyfEC&index=3&t=10s https://www.youtube.com/watch?v=mUyOpoDNe3Y&list=PL8tq3jQmKIAqWOzKn489NNL548ClOyfEC&index=5 https://www.youtube.com/playlist?list=PL8tq3jQmKIAqWOzKn489NNL548ClOyfEC https://www.youtube.com/watch?v=fhi4Ib0DH1Y&list=PL8tq3jQmKIAqWOzKn489NNL548ClOyfEC&index=11 Phipps Conservatory Science Corner Speaker | Aug. 2020 | Pittsburgh, PA Real Scientists Twitter Guest Curator | Jul. 2020 | Twitter https://medium.com/@realscientists/bright-spots-charlotte-hacker-hosts-real-scientists-3c10fc0513d4; https://twitter.com/realscientists/status/1284822539764748289 Just the Zoo of Us Podcast Guest | Jun. 2020 |Online https://anchor.fm/justthezooofus/episodes/57-Snow-Leopard-w-Charlotte-Hacker-efrck2 Phipps Conservatory Biophilia Network Speaker | May 2020 | Pittsburgh, PA https://www.youtube.com/watch?time_continue=49&v=6PKNfjN4Kds&feature=emb_logo #SciQFriday with Tyus Williams Guest | Apr. 2020 | Twitter https://twitter.com/sciencewithtyus/status/1253804148811796483 Exploring By The Seat Of Your Pants Guest Speaker | Jan. 2020 | Online https://www.youtube.com/watch?v=LI9cwTFeiKU Reach the World Traveler | Sept. – Dec. 2019 | Online https://reachtheworld.org/charlottes-journey-china DNA Day Guest Speaker | Apr. 2019 | Fort Couch Middle School | Upper St. Clair, PA Skype A Scientist | Nov. 2018 – Aug. 2019. | Online • Monee Elementary | May 2019 | Monee, IL • Cople Elementary | Apr. 2019 | Hague, VA • Brockwood Park High School | Mar. 2019 | Hampshire, UK Pittsburgh Zoo’s KidScience & Zoo U Program – Session on Big Cat Conservation | Dec. 2017 | Pittsburgh, PA

Technical Reports & Training Protocols (non Peer-Reviewed) Hacker C, Cancellare I, Weckworth B, Janecka JE. Micro-level design tools - Scat sampling protocol infographic and mini- manual. Submitted to Global Snow Leopard and Ecosystem Protection Program (GSLEP) for distribution | Oct. 2020 https://globalsnowleopard.org/gslep-projects/paws/paws-resources/micro-level-design-tools/

Hacker C. The perceived and functional effectiveness of foxlights in deterring predators from livestock on the Tibetan plateau – Submitted to the Chinese Academy of Forestry, The Snow Leopard Conservancy, and Foxlights Australia PTY LTD for distribution | Apr. 2020

Hacker C, Jebeckumar B, Janecka J. Results for noninvasive survey of snow leopard (Panthera uncia) scat samples from central Mongolia – Submitted for distribution | Jun. 2019

Hacker C, Janecka J. Results for noninvasive survey of snow leopard scat samples processed summer 2018 - Submitted to the Chinese Academy of Forestry for distribution | Jan. 2019

12 Charlotte Hacker Curriculum Vitae Updated Jul. 2021 (continued from previous page) Hacker C, Janecka J. Results for noninvasive survey of Changtang Reserve in Tibet and Qilianshan in Qinghai Province in relation to snow leopard phylogeography - Submitted to the Chinese Academy of Forestry for distribution | Mar. 2018

Hacker C. Stress Hormone Analysis of African elephants - Submitted to San Diego Zoo Safari Park |May 2014

Informal Science Writing • Dispatches from the Field | Oct. 2020 | Studying a species you’re not sure exists https://dispatchesfromthefield1.wordpress.com/2020/10/07/studying-a-species-youre-not-sure-exists/ • Oryx | Jun. 2020 | There’s something about snow leopards: assessing herder attitudes toward the ‘ghost of the mountains’ https://www.oryxthejournal.org/blog/theres-something-about-snow-leopards-assessing-herder-attitudes-toward-the-ghost-of-the-mountains/ • Reach the World | Sept. – Dec. 2019 | Charlotte’s Journey http://www.reachtheworld.org/charlottes-journey-china • Twitter | Oct. 2018 – Pres. | Approx. 1,300 followers |@chacker414 • Duquesne University Graduate Student Blog | Feb. 2017 | Seminar Blogs http://www.duq.edu/assets/Documents/biology/gsobs/student%20blogs/Hacker%20on%20Lane.1.pdf • Duquesne University Graduate Student Blog | Nov. 2016 | Game Changing Science in Pittsburgh www.duq.edu/assets/Documents/biology/gsobs/student%20blogs/Blog3.pdf • San Diego Zoo Blog | Apr. 2014 | An Enriched Elephant Herd blogs.sandiegozoo.org/2014/04/30/an-enriched-elephant-herd/ • San Diego Zoo Blog | Jan. 2014 | Tracking Safari Park Elephants blogs.sandiegozoo.org/2014/01/31/tracking-safari-park-elephants/`/.hg

International Experience Visiting Researcher |Jan. 2020 | Bengaluru (Bangalore), India • Met with research colleagues from the Snow Leopard Trust, Nature Conservation Foundation, and National Center for Biological Sciences to share information surrounding noninvasive genetics; presented a talk about current research efforts; networked with other PhD students, post-docs, research associates, and scientists. Fulbright Scholar | Jun. 2019 – Feb. 2020. | Chinese Academy of Forestry; Beijing Forestry University | Beijing, China • Led noninvasive scat surveys in the Kunlun Mountains of Dulan County, Qinghai Province China every two months to assess seasonal variation in snow leopard diet; conducted molecular dietary analysis; trained students in DNA extraction and sequencing; led forums on English scientific writing; navigated safety protocols, mandatory evacuation, and research challenges associated with the coronavirus outbreak. Visiting Researcher | Jun. – Aug. 2017, 2018; Dec. 2018 – Jan. 2019 | Chinese Academy of Forestry | Beijing, China • Conducted genetic analysis of snow leopard fecal samples from DNA extraction through genotyping; networked and instructed researchers; established collaborative efforts to relieve demand for geneticists; trained Tibetans in non-invasive scat sampling; learned basic Mandarin Chinese language, the importance of international collaborative efforts, information related to Asia’s wildlife, and conservation policies. Visiting Researcher |Jul. 2017 | Ulaanbaatar, Mongolia • Met with snow leopard research colleagues; learned about Mongolia’s action against the illegal wildlife trade, visited a BBC field site during an active documentary surrounding Pallas’ cats, and initiated transboundary cooperation between researchers in China, Mongolia, and the United States. Volunteer |Nov. - Dec 2010 | Imire Safari Ranch | Wedza, Zimbabwe • Participated in game counts, animal care to rescued Black rhinos and elephants, property maintenance, anti- poaching runs, animal tracking; learned information related to African mammals, elephant husbandry, adaptive skills for living in a rural landscape, agricultural and behavioral observation skills. Study Abroad |May – Nov. 2010 | University of Cape Town | Cape Town, South Africa • Spent a semester at the University of Cape Town; given many opportunities such as conservation hikes, scuba diving and history lessons; learned life skills such as adjusting to new environments, working around language barriers, and how to cope with different learning and teaching styles.

13 Charlotte Hacker Curriculum Vitae Updated Jul. 2021 (continued from previous page) Volunteer | Mar. 2010 | Alternative Spring Break | San Marcos, Costa Rica • Traveled to Costa Rica to a free trade coffee farm; picked coffee cherries, helped fix up a school, cut down old coffee plants, and analyzed the pros and cons of free trade coffee; learned manual skills, how to interact with locals despite language barriers, and how corporate American choices affect producers. Student | Jul. 2006 | Educational Tour of Europe | Austria, Czech Republic, Germany and Slovakia • Spent two weeks traveling to various significant and historical landmarks related to the class’s course content; learned European history, immersed myself in the German language, and realized the value in not just traveling, but of actively engaging in different cultures.

Relevant Coursework Graduate (Duquesne University): Advanced Cell and Molecular Biology; Science Ethics; Seminar; Introduction to Graduate Research; Bioinformatics; Conservation Physiology; Evolution of Sexual Selection; Global Biogeochemical Cycling; Elementary Chinese I (Carnegie Mellon University); Chinese Characters for Beginners (Coursera) Graduate (Western Kentucky University): Evolution and Ecological Genetics; Biometry; Mammalogy; Host-Parasite Associations; Advanced Ecology; Physiological Ecology; Graduate Seminar Undergraduate (University of Cape Town): Culture, Identity, and Globalization in Africa; International Politics; Philosophical Ethics Undergraduate (Wilkes University): Animal Behavior; Comparative Vertebrate Anatomy; Conservation Biology; Developmental Biology; Population and Evolutionary Biology; Professional Prep Techniques

In the Media • Trombola N. May 2020. For Fulbright Recipient and Duquesne Student, Pandemic Creates Hectic Escape from Asia. The Pittsburgh Post-Gazette. https://www.post-gazette.com/news/education/2020/05/07/Charlotte-Hacker-Fulbright-scholarship-Duquesne-student-snow-leopard-study-pandemic- Covid-19-Asia-China-escape/stories/202005060159 • Kidd S. Feb. 2020. New Jersey 4th-Graders Connect with Researcher Charlotte Hacker & The Conservancy. Snow Leopard Conservancy. http://hosted-p0.vresp.com/511631/b51cac5ad1/ARCHIVE • Kidd S. Feb. 2020. Studying the Use of Guard Dogs as a Predator Deterrent in China. Snow Leopard Conservancy. https://snowleopardconservancy.org/2020/02/02/studying-the-use-of-guard-dogs-as-a-predator-deterrent-in-china/ • 46 Questions. Jan. 2020. Charlotte Hacker. 46 Questions for Scientists. https://46questions.wordpress.com/2020/01/19/charlotte-hacker/ • Valkanas M. Jan. 2020. Conservation Geneticist Uses Scat to Gain Insight on the Elusive Snow Leopard. Science in my Backyard. DuQuark. https://duquark.com/2020/01/09/conservation-geneticist-uses-scat-to-gain-insight-on-the-elusive-snow-leopard/ • Kidd S. Dec. 2019. Looking Toward a Hopeful Future for Snow Leopards. Snow Leopard Conservancy. https://snowleopardconservancy.org/2019/12/29/looking-toward-a-hopeful-future-for-snow-leopards/ • #UniqueScientists. Nov. 2019. Unique Scientists – Charlotte Hacker. Unique Scientists. https://uniquescientists.com/2019/11/28/unique-scientist-charlotte-hacker/ • Harris M, Koscinski K, Gavin K. May 2019. The Confluence. Pittsburgh NPR. Min. 23 – 34. https://www.wesa.fm/post/what-pa-republicans-lack-messaging-they-make-presidency-state-party-chair-says • Walters K. Apr. 2019. Duquesne University Fulbright Scholar to Student Snow Leopards in China. Duquesne University https://www.duq.edu/news/duquesne-university-fulbright-scholar-to-study-snow-leopards-in-china • Wright C. Mar. 2019. Protecting the Snow Leopard: Q & A – Charlotte Hacker. NatureVolve Magazine. https://www.dropbox.com/s/8dqqamixru2eilp/NV%20issue%203%20pdf.pdf?dl=0 • Xim N. Feb. 2019. Where the Snow Leopards are, Charlotte. Conflict & Coexistence, Carnivores & Humans. https://toowild.wixsite.com/carnivoresandhumans/blog-1/where-the-snow-leopards-are-charlotte • Kobilinsky D. Nov. 2018. Student Travel Grants Bring 17 Wildlife Students to Cleveland. The Wildlife Society. http://www.wildlife.org/student-travel-grants-bring-17-wildlife-students-to-cleveland/?platform=hootsuite • Lukas J. Jul. 2017. Charlotte Hacker ’12. Wilkes University Alumni Spotlight. http://community.wilkes.edu/s/344/index.aspx?sid=344&gid=1&pgid=1988&cid=3852&ecid=3852&crid=0&calpgid=508&calcid=3522

14 Charlotte Hacker Curriculum Vitae Updated Jul. 2021 (continued from previous page) • Osborne H. May 2017. Snow Leopards: Scientists Discover Three New Subspecies of the World’s Most Elusive Big Cat. Newsweek Magazine. http://www.newsweek.com/snow-leopard-subspecies-discovered-conservation-elusive-big-cat-608381 • AZA. Jun. 2015. Using GPS to Evaluate Relationships Among Social Animals. Connect Magazine. https://www.speakcdn.com/assets/2332/research-abstracts-connect.pdf