DOCSLIB.ORG

Chapter D1 Ion Optics of Magnetic/Electric Sector Ms

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Chapter D1 Ion Optics of Magnetic/Electric Sector Ms Back to Basics Ion Optics of Magnetic/Electric Sector MS Back to Basics Section D: Ion Optics CHAPTER D1 ION OPTICS OF MAGNETIC/ELECTRIC SECTOR MS TABLE OF CONTENTS QuickGuide ...........................369 Summary..............................369 Preamble..............................371 MassAnalysisofIons....................371 MagneticSector.......................371 ElectrostaticAnalyser(ElectricSector).....375 Magnet / Electrostatic Analyser Combination 375 ElectricFocusingLenses .................377 Y-Focus,Z-FocusandDeflectLenses .....377 Curvature and Rotation Lenses . 379 MetastableIons.........................379 EnergyFilter .........................379 Conclusion ............................379 Micromass UK Limited Page 367 Back to Basics Ion Optics of Magnetic/Electric Sector MS This page is intentionally blank. Micromass UK Limited Page 368 Back to Basics Ion Optics of Magnetic/Electric Sector MS Quick Guide • Substances are converted into species having positive or negative charges (ions) in the ion source. • For an ion of mass (m) and a number (z) of positive or negative charges, the value m/z is an important mass spectrometric observable. • Astreamofions(an ion beam) is directed out of the ion source towards a collector which records their arrival. • As with a light beam and glass lenses, an ion beam can be directed and focused using electric and magnetic fields, often called lenses by analogy with their optical counterparts. • The system of electric and magnetic fields or lenses is called the ion optics of the mass spectrometer. • Electric lenses correct aberrations in the shape of the ion beam. • Electric and magnetic fields can be used sequentially, as described in this issue. Crossed electromagnetic fields are described in the separate issue on quadrupoles. • Another important property of electric and magnetic fields is their ability to separate ions according to their individual masses (m1,m2 ..... mn) or, more strictly, their mass-to-charge ratio (m1/z, m2/z ..... mn/z). • After the ion source, the ion optics split the ion beam into its component m/z values (compare splitting white light into a spectrum of colours). • By changing the strengths of the electric and magnetic fields, ions of different m/z values can be focused at just one spot (the collector). • From the strengths of the electric and magnetic fields, m/z values are measured. • A chart showing the number of ions (abundance) arriving at the collector and their respective m/z values is a mass spectrum. Summary The ion optics of a mass spectrometer cause the ion beam leaving the ion source to arrive at a collector after being separated into individual m/z values and focused. Micromass UK Limited Page 369 Back to Basics Ion Optics of Magnetic/Electric Sector MS Field Current Direction of Deflection Figure 1 Fleming’s Left Hand Rule Micromass UK Limited Page 370 Back to Basics Ion Optics of Magnetic/Electric Sector MS ION OPTICS OF MAGNETIC/ELECTRIC SECTOR MASS SPECTROMETERS Preamble In the ion source, substances are converted into positive or negative ions having masses (m1,m2, ..... mn) and a number (z) of electric charges. From a mass spectrometric viewpoint, the ratio of mass to charge (m1/z, m2/z ....... mn/z) is important. Generally, z =1,inwhich case, m1/z = m1,m2/z = m2 ..... mn/z = mn, so that the mass spectrometer measures masses of ions. To do this a stream of ions (the ion beam) is injected into the mass analyser region, a series of electric and magnetic fields known as the ion optics. In this region, the ion beam is focused, corrected for aberrations in shape and the individual m/z ratios measured. The ion beam finally arrives at a collector which measures the number (abundance) of ions at each m/z value. The width and shape of the ion beam is controlled by a series of slits (object or source, collector, alpha etc.), situated between the ion source and the collector. A chart of m/z values and their respective abundances makes up the mass spectrum. Ion optics are considered in greater detail below. Mass Analysis In this section, magnetic and electric sectors and electric focusing of Ions lenses are discussed. Magnetic Sector When moving charged species (ions) experience a magnetic field, they are deflected. The direction of the deflection can be described by Fleming's ‘left hand rule’ (Figure 1). Themagnitudeofthedeflectionisgovernedbythemomentumofthe ion and is described by the following equations (1,2). Firstly, the kinetic energy of the ion is equal to the energy gained through acceleration from the ion source (equation 1). 1 zV = ---mv2 (1) 2 Secondly, the centrifugal force on the ion as its path is deflected by a magnetic field is equal to the force exerted by the field on a moving charge (equation 2). mv -------- = zB (2) r Micromass UK Limited Page 371 Back to Basics Ion Optics of Magnetic/Electric Sector MS 5 4 3 Ion beam Magnet 2 1 Deflected ions Figure 2 Deflection in a magnetic field of an ion beam consisting of increasing mass-to-charge ratios, m1/z....m5/z and split into different trajectories (1-5) respectively Object Slit α - Slit Focused Ion Beam (Collector Slit) Figure 3 Directional (or angular) focusing of a magnet. Micromass UK Limited Page 372 Back to Basics Ion Optics of Magnetic/Electric Sector MS From equations (1,2), the velocity of the ion can be eliminated to give the relationship (3). m B2r2 ----- = ------------ (3) z 2V Where: r = radius of arc of ions being deflected in the magnetic field V = accelerating potential applied to ions leaving the ion source B = magnetic field strength z = number of charges on an ion m = mass of any one ion v = velocity of an ion after acceleration through the electric field (V). If only ions with a single charge (z =1) are considered then, with a constant field strength and constant accelerating voltage, the radius of arc depends on mass and, from (3), equation (4) is obtained. 2V rm= ------- (4) B2 Thus, it is possible to separate ions of different mass (Figure 2) with ions arriving at position 1 (greater deflection) being of lower mass than those arriving at position 5 (lesser deflection). In the modern scanning mass spectrometer, it is more convenient that ions should arrive at a single point for monitoring (collection) and so r (or r2)is kept constant. This means that B and/or V must be varied to bring all ions to the same focus, viz., one of the relationships (5) must apply: m ∝ B2 (V constant) m ∝ 1/V (B constant) m ∝ B2/V (5) From these relationships, (5), it can be seen that, if either the magnetic field (B) or the voltage (V) or both B and V are scanned, the whole range of masses of the ions may be brought into focus sequentially at a given point, the collector. Generally, a scanning magnetic sector mass spectrometer carries out mass analysis by keeping V constant and varying the magnetic field (B). A further property of the magnetic field is that a diverging ion beam entering that field leaves with the beam converging. Thus, the magnet is said to be directional (or angular) focusing (Figure 3). Micromass UK Limited Page 373 Back to Basics Ion Optics of Magnetic/Electric Sector MS (LOW ENERGY IONS) (HIGH ENERGY IONS) OUTER ESA PLATE SLIT INNER ESA PLATE Y FOCUS SLIT SOURCE SLIT (VARIABLE) Figure 4 Focusing and dispersion properties of an electrostatic analyser. Micromass UK Limited Page 374 Back to Basics Ion Optics of Magnetic/Electric Sector MS So far, it has been assumed that all ions leaving the source have exactly the same kinetic energy but this is not really the case. In EI, the spread in kinetic energy can be as much as 1 volt and, with FAB can be as much as 4 volts. This spread results in a blurred image at the collector because the magnet has no energy focusing and ions of different kinetic energies are brought to slightly different foci. Thus a single magnetic sector has directional (or angular) focusing and therefore is said to be single focusing only. Electrostatic Analyser An electrostatic analyser (ESA) is a directional (or angular) focusing (Electric Sector) device and is also energy dispersive (Figure 4). As shown in equations (1,2), the energy gained by ions accelerated 1 from the ion source iszV = ---mv2 and in the electric sector, the 2 centrifugal force acting on the ions is given by equation (6), mv2 zE = ----------- (6) R where: E = electric potential (voltage) between the inner and outer ESA plates. R = radius of curvature of ion trajectory From these equations, the relationship (7) is obtained. 2V R = ------- (7) E No mass or charge appears in this equation so that, in the electric sector, the ion flight path bends in an arc, which depends only on the accelerating voltage (V) and the ESA voltage (E). Magnet / Electrostatic The ion beam is collimated when a magnetic analyser is combined Analyser Combination with an ESA, the combination can be made both energy and mass focused, vis., the ion beam is collimated in the ESA and then properly focused in the magnetic field (Figure 5). The combination is called double focusing because it is both directional (or angular) and energy focusing. The double focusing mass spectrometer is designed such that ions of different energies (but of the same mass), converge at the collector (Figure 5). Double Focusing Forward Geometry ion optics is a combination, in which the ESA is placed before the magnet as shown in Figure 5. Micromass UK Limited Page 375 Back to Basics Ion Optics of Magnetic/Electric Sector MS (HIGH ENERGY IONS) (IONS OF TUNED MASS) COLLECTOR SLIT OUTER ESA PLATE ELECTROMAGNET (LOW ENERGY IONS) β SLIT INNER ESA PLATE Y FOCUS α SLIT SOURCE SLIT (VARIABLE) SOURCE SLIT LENS (FIXED) FOCUS (BEAM CENTRE) SOURCE EI/CI Figure 5 Double focusing ion optics (forward geometry).
Load more

Recommended publications
  • About Magnetic Sector Mass Spec- Trometers
    About Magnetic Sector Mass Spec- trometers Double-focusing magnetic sector mass spectrometers provide high sensitivity, high resolution, and a reproducibility that is unmatched in any other kind of mass analyzer. A double-focusing magnetic sector mass spectrometer has at least four components: . An ion source in which ions are formed and accelerated to energies of up to as high as 10 kilovolts. A magnetic sector with a magnetic field that exerts a force perpendicular to the ion mo- tion to deflect ions according to their momentum. An electric sector with an electric field that exerts a force perpendicular to the ion mo- tion to deflect ions according to their kinetic energy. A detector that produces a response that is proportional to the number of ions. Slits are placed in the ion path to define the positions and energies of the ions that strike the detector. In general, decreasing the slit widths increases the mass resolution but re- duces the number of ions that are detected. Additional electrostatic lenses are com- monly used to shape and deflect the ion beam to optimize peak shape and maximize ion beam transmission from the source to the detector. JEOL uses octapole and quadrupole focusing lenses to simplify the ion optical design. Collision chambers in the first field-free region (just after the ion source) and second field-free region (just after the magnet and before the electric sector) are used to induce ions to fragment in collision-induced-dissociation experiments (MS/MS). The term "double-focusing" refers to the fact that the combination of electric and mag- netic sectors focuses ions according to both direction and energy to provide higher reso- lution than can be obtained with a single magnetic sector.
    [Show full text]
  • An Introduction to Mass Spectrometry
    An Introduction to Mass Spectrometry by Scott E. Van Bramer Widener University Department of Chemistry One University Place Chester, PA 19013 [email protected] http://science.widener.edu/~svanbram revised: September 2, 1998 © Copyright 1997 TABLE OF CONTENTS INTRODUCTION ........................................................... 4 SAMPLE INTRODUCTION ....................................................5 Direct Vapor Inlet .......................................................5 Gas Chromatography.....................................................5 Liquid Chromatography...................................................6 Direct Insertion Probe ....................................................6 Direct Ionization of Sample ................................................6 IONIZATION TECHNIQUES...................................................6 Electron Ionization .......................................................7 Chemical Ionization ..................................................... 9 Fast Atom Bombardment and Secondary Ion Mass Spectrometry .................10 Atmospheric Pressure Ionization and Electrospray Ionization ....................11 Matrix Assisted Laser Desorption/Ionization ................................ 13 Other Ionization Methods ................................................13 Self-Test #1 ...........................................................14 MASS ANALYZERS .........................................................14 Quadrupole ............................................................15
    [Show full text]
  • Compositional Analysis of Complex Organic Mixtures by Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Zhigang Wu
    Florida State University Libraries Electronic Theses, Treatises and Dissertations The Graduate School 2004 Compositional Analysis of Complex Organic Mixtures by Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Zhigang Wu Follow this and additional works at the FSU Digital Library. For more information, please contact [email protected] THE FLORIDA STATE UNIVERSITY COLLEGE OF ARTS AND SCIENCES Compositional Analysis of Complex Organic Mixtures by Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry By Zhigang Wu A Dissertation submitted to the Department of Chemistry and Biochemistry in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy Degree Awarded: Spring Semester, 2004 Copyright2004 Zhigang Wu All Rights Reserved The members of the Committee approve the Dissertation of Zhigang Wu Defended on April 7, 2004. Alan G. Marshall Professor Directing Dissertation William M. Landing Outside Committee Member William T. Cooper Committee Member Timothy A. Cross Committee Member Ryan P. Rodgers Committee Member The Office of Graduate Studies has verified and approved the above named committee members. ii To my parents, Xiaohua Wu and Shiping Cao; iii ACKNOWLEDGMENTS First, I need to thank Dr. Alan G. Marshall, my academic graduate advisor and supporter. His deep and broad academic knowledge and great personality are invaluable throughout my graduate career. His wise and forecasting advices has helped me directly cut to the “point” and inspired me to discover more underneath. I also wish to acknowledge Dr. Ryan P. Rodgers, my supervisor, for his expertise, direction, and support. His initiation and development in petroleum applications have led me to a broader and better understanding of this area.
    [Show full text]
  • 82756031.Pdf
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector Do Charge-Remote Fragmentations Occur under Matrix-Assisted Laser Desorption Ionization Post-Source Decompositions and Matrix-Assisted Laser Desorption Ionization Collisionally Activated Decompositions? M. Rosario M. Domingues, M. Grac¸a O. S.-Marques, Carla A. M. Vale, M. Grac¸a Neves, J. A. S. Cavaleiro, and A. J. Ferrer-Correia Department of Chemistry, University of Aveiro, Aveiro, Portugal Olga V. Nemirovskiy and Michael L. Gross Department of Chemistry, Washington University, St Louis, Missouri, USA The precursor ions of tetraphenylporphyrins that are substituted with fatty acids can be introduced into the gas phase by matrix-assisted laser desorption ionization (MALDI) and undergo post-source and collisionally activated decompositions (CAD) in a time-of-flight mass spectrometer. The goal of the research is to obtain a better understanding of post-source decompositions (PSD); specifically, we asked the question of whether ions undergoing PSD have sufficient energy to give charge-remote fragmentations along an alkyl chain. We chose the porphyrin macrocycle because we expected it to act as an inert “support,” allowing the molecule to be desorbed by MALDI and to be amenable to charge-remote fragmentation. MALDI-PSD and MALDI-CAD spectra are similar to high-energy CAD spectra and consid- erably more informative than low-energy CAD spectra, showing that charge-remote fragmen- tations of the fatty acid moieties do occur upon MALDI-PSD and MALDI-CAD. (J Am Soc Mass Spectrom 1999, 10, 217–223) © 1999 American Society for Mass Spectrometry ost-source decompositions (PSD) of ions pro- ever, observed different fragmentation pathways in duced by matrix-assisted laser desorption (MALDI) MALDI-PSD spectra when compared with those ob- Phas become a valuable structural tool for peptides served in LE-CAD and HE-CAD spectra.
    [Show full text]
  • Chemical Analysis in the Titan Environment
    • Magnetic Sector Mass Spectrometer • Quadrupole mass spectrometer • Quadrupole Ion Traps • Orthogonal Sampling Time of Flight Mass Spectrometer • Fourier Transform MS: Orbitrap Mass Spectrometer • Simulated Titan Chemistry (discharge and VUV) • Spectroscopic characteriza;on • Separa;on and analysis • Structural chemistry • Reac;vity (introduc;on of oxygen) Gas inlet Discharge Source UV Photolysis (ALS) Nier’s ‘mass spectrometer in a briefcase’, constructed in the mid-1960s, was used to convince NASA officials that it was possible to include such instrumentation on spacecraft. Miniature focal plane mass spectrometer with 1000-pixel modified-CCD detector array for direct ion measurement Mahadeva P. Sinha and Mark Wadsworth Rev. Sci. Instrum. 76, (2005); doi:10.1063/1.1840291 Schema'c of the focal plane mass spectrometer of Ma2auch–Herzog geometry Table I. MMS features and performance MMS Mass 395 g (as shown in the photograph) Dimensions 10 cm × 5 cm × 5 cm Ion source Thermionic Mass range 2–250 ResoluQon 330 (50% valley definiQon) SensiQvity 2 μA/Torr Photograph of the miniature mass spectrometer The spectrum measured by the transla'on of a single detector element along the focal plane. The peaks correspond to the residual gases in the vacuum chamber and the doubly charged ions arising from krypton gas introduced into the chamber. Rev. Sci. Instrum. 76, 025103 (2005) © 2005 American InsQtute of Physics A quadrupole mass filter consists of four parallel metal rods. Two opposite rods have an applied potenQal of (U+Vcos(wt)) and the other two rods have a potenQal of -(U+Vcos (wt)), where U is a dc voltage and Vcos(wt) is an ac voltage.
    [Show full text]
  • FACTORS AFFECTING the FRAGMENTATION of PEPTIDE IONS: METAL CATIONIZATION and FRAGMENTATION TIMESCALE a Dissertation by KEVIN LE
    FACTORS AFFECTING THE FRAGMENTATION OF PEPTIDE IONS: METAL CATIONIZATION AND FRAGMENTATION TIMESCALE A Dissertation by KEVIN LEE KMIEC Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY August 2012 Major Subject: Chemistry Factors Affecting the Fragmentation of Peptide Ions: Metal Cationization and Fragmentation Timescale Copyright 2011 Kevin Lee Kmiec FACTORS AFFECTING THE FRAGMENTATION OF PEPTIDE IONS: METAL CATIONIZATION AND FRAGMENTATION TIMESCALE A Dissertation by KEVIN LEE KMIEC Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Approved by: Chair of Committee, David H. Russell Committee Members, Paul E. Hardin Simon W. North Emile A. Schweikert Head of Department, David H. Russell August 2012 Major Subject: Chemistry iii ABSTRACT Factors Affecting the Fragmentation of Peptide Ions: Metal Cationization and Fragmentation Timescale. (August 2012) Kevin Lee Kmiec, B.A., Hendrix College Chair of Advisory Committee: Dr. David H. Russell The factors affecting peptide fragmentation have been extensively studied in the literature in order to better predict the fragment ion spectra of peptides and proteins. While there are countless influences to consider, metal cation binding in the gas-phase is particularly interesting. Herein, a comparison of fragmentation patterns of a model peptide series with various charge carriers (H+, Li+, Na+, K+, and Cu+) will assist in determining the location of the preferred binding site of the metal cation and in assessing differences in the fragmentation pattern as a result of this binding site.
    [Show full text]
  • Summary of the Characteristics of Different Mass Analyzers
    Summary of the characteristics of different mass analyzers All mass spectrometers combine ion formation, mass analysis, and ion detection. This discussion is concerned with how various mass analyzers are used to separate ions according to their mass- to-charge ratio. Each mass analyzer has its own special characteristics and applications and its own benefits and limitations. The choice of mass analyzer should be based upon the application, cost, and performance desired. There is no ideal mass analyzer that is good for all applications.For an excellent and more complete discussion of mass analyzers, see "The Ideal Mass Analyzer: Fact or Fiction?" (Curt Brunnee, Int. J. Mass Spectrom. Ion Proc. 76 (1987), 125-237. Note that different mass analyzers use different resolution definitions. See "How Resolution is Defined" for a discussion of this topic. Note also that a complete discussion of MS/MS methods is beyond the scope of this essay, but some comments about MS/MS are provided here in the context of comparing different mass ana- lyzers strengths and weaknesses. General: The effect of electromagnetic fields on ions All commonly used mass analyzers use electric and magnetic fields to apply a force on charged particles (ions). The relationship between force, mass, and the applied fields can be summarized in Newton's second law and the Lorentz force law: F = ma (Newton's second law) F= e(E+ v x B) (Lorentz force law) where . F is the force applied to the ion, . m is the mass of the ion, . a is the acceleration, . eis the ionic charge, . E is the electric field .
    [Show full text]
  • The Development of Structural Mass Spectrometry Based Techniques for the Study of Aggregation-Prone Proteins
    The development of structural mass spectrometry based techniques for the study of aggregation-prone proteins. Owen Cornwell School of Molecular and Cellular Biology Astbury Centre for Structural Molecular Biology University of Leeds Submitted in accordance with the requirements for the degree of Doctor of Philosophy December 2019 Declaration The candidate confirms that the submitted work is his own and that appropriate credit has been given within the thesis where reference has been made to the work of others. This copy has been supplied on the understanding that it is copyright material and that no quotation from this thesis may be published without proper acknowledgement. © 2019 The University of Leeds and Owen Cornwell i ii Jointly Authored Publications Chapters 4 and 5 contain work from the following published manuscript: Cornwell, O., et al., Comparing Hydrogen Deuterium Exchange and Fast Photochemical Oxidation of Proteins: a Structural Characterisation of Wild-Type and ΔN6 β2-Microglobulin. Journal of The American Society for Mass Spectrometry, 2018. 29(12): p. 2413-2426. In this work, all experimental work and data analysis was performed by OC. Optimisation of the HDX workflow and associated method development was performed by OC and JRA. Initial development and coding of the novel processing algorithm was performed by OC. Final development and coding of the PAVED software available for download was performed by JRA. Chapter 6 contains work from the following published manuscript: Cornwell, O., et al., Long-Range Conformational Changes in Monoclonal Antibodies Revealed Using FPOP-LC-MS/MS. Analytical Chemistry, 2019. 91(23): p. 15163- 15170. In this work, all experimental work and data analysis was performed by OC.
    [Show full text]
  • Hybrid Mass Spectrometers for Tandem Mass Spectrometry
    FOCUS: COOKS, 2006 DISTINGUISHED CONTRIBUTION IN MASS SPECTROMETRY AWARDEE ACCOUNT AND PERSPECTIVE Hybrid Mass Spectrometers for Tandem Mass Spectrometry Gary L. Glisha and David J. Burinskyb a Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina, USA b GlaxoSmithKline, Five Moore Drive, P.O. Box 13398, Research Triangle Park, North Carolina, USA Mass spectrometers that use different types of analyzers for the first and second stages of mass analysis in tandem mass spectrometry (MS/MS) experiments are often referred toas “hybrid” mass spectrometers. The general goal in the design of a hybrid instrument is to combine different performance characteristics offered by various types of analyzers into one mass spectrometer. These performance characteristics may include mass resolving power, the ion kinetic energy for collision-induced dissociation, and speed of analysis. This paper provides a review of the development of hybrid instruments over the last 30 years for analytical applications. (J Am Soc Mass Spectrom 2008, 19, 161–172) © 2008 American Society for Mass Spectrometry andem mass spectrometry (MS/MS), in a very “high” ion kinetic energies (5–20 keV), whereas “low” generic description, is a process in which an ion ion kinetic energies (Ͻ50 eV) are typical in quadrupole Tformed in anion source is mass-selected in the mass filters and ion traps. The ion kinetic energy is an firststage of analysis, reacted, and thenthe charged important parameter in MS/MS experiments because products from the reaction are analyzed in the second the most common reaction involves colliding the ion of stage of analysis. The type and quality of data that is interest with a target gas atom or molecule.
    [Show full text]
  • LIQUID CHROMATOGRAPHY– MASS SPECTROMETRY: an INTRODUCTION Analytical Techniques in the Sciences (Ants)
    Liquid Chromatography –Mass Spectrometry: An Introduction. Robert E. Ardrey Copyright ¶ 2003 John Wiley & Sons, Ltd. ISBNs: 0-471-49799-1 (HB); 0-471-49801-7 (PB) LIQUID CHROMATOGRAPHY– MASS SPECTROMETRY: AN INTRODUCTION Analytical Techniques in the Sciences (AnTS) Series Editor: David J. Ando, Consultant, Dartford, Kent, UK A series of open learning/distance learning books which covers all of the major analytical techniques and their application in the most important areas of physical, life and materials science. Titles Available in the Series Analytical Instrumentation: Performance Characteristics and Quality Graham Currell, University of the West of England, Bristol, UK Fundamentals of Electroanalytical Chemistry Paul M.S. Monk, Manchester Metropolitan University, Manchester, UK Introduction to Environmental Analysis Roger N. Reeve, University of Sunderland, UK Polymer Analysis Barbara H. Stuart, University of Technology, Sydney, Australia Chemical Sensors and Biosensors Brian R. Eggins, University of Ulster at Jordanstown, Northern Ireland, UK Methods for Environmental Trace Analysis John R. Dean, Northumbria University, Newcastle, UK Liquid Chromatography–Mass Spectrometry: An Introduction Robert E. Ardrey, University of Huddersfield, Huddersfield, UK Forthcoming Titles Analysis of Controlled Substances Michael D. Cole, Anglia Polytechnic University, Cambridge, UK LIQUID CHROMATOGRAPHY– MASS SPECTROMETRY: AN INTRODUCTION Robert E. Ardrey University of Huddersfield, Huddersfield, UK Copyright 2003 John Wiley & Sons Ltd, The Atrium, Southern
    [Show full text]
  • Instrumentation and Development of a Mass Spectrometry System for the Study of Gas-Phase Biomolecular Ion Reactions Ziqing Lin Purdue University
    Purdue University Purdue e-Pubs Open Access Dissertations Theses and Dissertations Winter 2015 Instrumentation and development of a mass spectrometry system for the study of gas-phase biomolecular ion reactions Ziqing Lin Purdue University Follow this and additional works at: https://docs.lib.purdue.edu/open_access_dissertations Part of the Biomedical Engineering and Bioengineering Commons, and the Chemistry Commons Recommended Citation Lin, Ziqing, "Instrumentation and development of a mass spectrometry system for the study of gas-phase biomolecular ion reactions" (2015). Open Access Dissertations. 503. https://docs.lib.purdue.edu/open_access_dissertations/503 This document has been made available through Purdue e-Pubs, a service of the Purdue University Libraries. Please contact [email protected] for additional information. INSTRUMENTATION AND DEVELOPMENT OF A MASS SPECTROMETRY SYSTEM FOR THE STUDY OF GAS-PHASE BIOMOLECULAR ION REACTIONS A Dissertation Submitted to the Faculty of Purdue University by Ziqing Lin In Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy May 2015 Purdue University West Lafayette, Indiana ii ACKNOWLEDGEMENTS I am deeply thankful to my PhD advisor, Prof. Zheng Ouyang. He is more than a mentor or supervisor, but a kind friend, giving me a fantastic PhD experience at Purdue. His passion, courage and extraordinary vision in scientific research makes him an outstanding scientist and engineer. Prof. Ouyang works hard day and night, while on the other side providing a free and comfortable environment for me and other colleagues in the group to do research and raise opinions without pressure. These precious characteristics no doubt affect me in my professional life. When we first met in Tsinghua, he told me that PhD life is a best opportunity to test our boundary of capabilities.
    [Show full text]
  • An Introduction to Ion Optics for the Mass Spectrograph
    AN INTRODUCTION TO ION OPTICS FOR THE MASS SPECTROGRAPH Thomas W. Burgoyne and Gary M. Hieftje* Department of Chemistry, Indiana University, Bloomington, Indiana 47405 Abstract .................................................................................................................... 24 1 I. Introduction ............................................................................................................ 24 1 11. Mass Spectrograph Ion Optics.. ........................................................................................ 243 A. Overview, Symbols, and Coordinates.. .............................................................................. 243 B. Transfer Matrix Method.. ........................................................................................... 243 C. Matrix Definitions for Mass Spectrograph Components. ............................................................. 244 D. Double Focusing for a Single Reference Mass (Focal Point). ........................................................ 247 E. Double Focusing for Several Masses (Focal Line). .................................................................. 247 111. Mass Spectrograph Geometries ......................................................................................... 248 A. Bainbridge-Jordan. .................................................................................................. 249 B. Mattauch-Herzog ................................................................................................... 249 C. Nier-Johnson.
    [Show full text]