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CCP110 Antibody (Pab)

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CCP110 Antibody (Pab) 21.10.2014CCP110 antibody (pAb) Rabbit Anti-Human/Mouse/Rat Centriolar coiled coil protein 110 (Centriolin, CNTRL, FAN, CEP1, CEP110) Instructio n Manual Catalog Number PK-AB718-6825 Synonyms CCP110 Antibody: Centriolar coiled coil protein 110, Centriolin, CNTRL, FAN, CEP1, CEP110 Description CCP110 is a centrosomal protein required for the centrosome to function as a microtubule organizing center and plays an essential role in centrosome duplication. It is a target of cyclin- dependent kinases (CDKs); disruption of CCP110 phosphorylation leads to unscheduled centrosome separation and polyploidy. CCP110 is also a target of SCF (Cyclin F) ubiquitin ligase complex, leading to its degradation, suggesting that this complex controls centrosome homeostasis and mitotic fidelity through CCP110 degradation. The centriolar kinesin Kif24 also interacts with CP110 and remodel microtubules and regulate ciliogenesis. Quantity 100 µg Source / Host Rabbit Immunogen CCP110 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human CCP110. Purification Method Affinity chromatography purified via peptide column. Clone / IgG Subtype Polyclonal antibody Species Reactivity Human, Mouse, Rat Specificity Two isoforms of CCP110 are known to exist; this antibody will only detect the longer isoform Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Storage & Stability Antibody can be stored at 4ºC for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Applications E, WB, IHC, IF |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined indivudually. CCP110 antibody can be used for detection of CCP110 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 5 µg/mL. Images Available upon request. References Chen Z, Indjeian VB, McManus M, et al. CP110, a cell cycle-dependent CDK substrate, regulates centrosome duplication in human cells. Dev. Cell 2002; 3:339-50. D’Angiolella V, Donato V, Vijayakumar S, et al. SCF (Cyclin F) controls centrosome homeostasis and mitotic fidelity through CP110 degradation. Nature 2010; 466:138-42. Kobayashi T, Tsang WY, Li J, et al. Centriolar kinesin Kif24 interacts with CP110 to remodel microtubules and regulate ciliogenesis. Cell 2011; 145:914-25. Images Available upon request. Related Products Cat. No. PK-AB718-6825P - CCP110 Peptide Cat. No. PK-AB718-1320 - Human Colon Tissue Lysate FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PROCEDURES. PromoCell GmbH North America 1 – 866 – 251 – 2860 (toll free) Email: [email protected] Sickingenstr. 63/65 Deutschland 0800 – 776 66 23 (gebührenfrei) www.promokine.info 69126 Heidelberg France 0800 90 93 32 (ligne verte) Germany United Kingdom 0800 – 96 03 33 (toll free) Other Countries +49 6221 – 649 34 0 09/2014 .
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