The Pennsylvania State University Schreyer Honors College

THE PENNSYLVANIA STATE UNIVERSITY SCHREYER HONORS COLLEGE

DEPARTMENT OF BIOLOGY

MOLECULAR PHYLOGENY AND BIOGEOGRAPHY OF NEOTROPICAL LIZARDS OF THE FAMILY DIPLOGLOSSIDAE

STEPHANIE L. DENNISON

Spring 2010

A thesis submitted in partial fulfillment of the requirements for a baccalaureate degree in Biology with honors in Biology

Reviewed and approved* by the following:

S. Blair Hedges Professor of Biology Thesis Supervisor

Daniel J. Cosgrove Professor of Biology Honors Adviser

* Signatures are on file in the Schreyer Honors College

ABSTRACT

The lizard family Diploglossidae is distributed in the West Indies, Central

America, and South America. In English speaking countries they are known as galliwasps. As part of an ongoing investigation of the historical biogeography of the West

Indies, the phylogenetic relationships and biogeography of these Neotropical lizards were studied. Although several morphological and molecular studies have been conducted in the past, they were limited in scope. Consequently, the phylogenetic relationships, taxonomy, and biogeography of diploglossids remain controversial. The purpose of this thesis, therefore, is to obtain a better understanding of the phylogeny and biogeography of the lizard family Diploglossidae.

New DNA sequences were collected from three mitochondrial genes (ND2, 12S, and 16S ribosomal RNA) and one nuclear gene (RAG1) for up to 145 diploglossid lizards and one outgroup species, and subjected to phylogenetic analyses. Based on these analyses, two separate West Indian and Central American lineages of Celestus and

Diploglossus were identified, the two tetradactyl genera Sauresia and Wetmorena were found to be nested among West Indian Celestus, and many subspecies and populations of the Genus Celestus and Diploglossus were found to form distinct evolutionary lineages that might warrant recognition of full species.

In order to further resolve diploglossid molecular phylogeny, future research in this area should focus on filling in the gaps in the mitochondrial and nuclear sequence data set, as well as continuing to add specimens. In addition, morphological characters should be examined to determine whether some genetically distinct subspecies should be

elevated to species. Finally, molecular divergence times should be determined in order to make more specific conclusions regarding biogeography.

TABLE OF CONTENTS

ABSTRACT ...... i

INTRODUCTION ...... 1

Biogeography of the West Indies ...... 1 Diploglossidae...... 2

MATERIALS AND METHODS ...... 7

Molecular Methods ...... 7 Phylogenetic Analysis ...... 11

RESULTS AND DISCUSSION ...... 12

FIGURES AND TABLES ...... 19

Table 1 ...... 19

Figure 1 ...... 20

Figure 2 ...... 26

Figure 3 ...... 27

ACKNOWLEDGEMENTS ...... 28

REFERENCES ...... 29

APPENDICES ...... 32

iii

A. Specimen Localities ...... 32

B. 12S, 16S, ND2, RAG1 Primers ...... 35

C. 12S, 16S rRNA mtDNA Sequence Data ...... 37

D. ND2 mtDNA Sequence Data ...... 95

E. RAG1 nucDNA Sequence Data ...... 177

INTRODUCTION

Biogeography of the West Indies

The West Indies is an archipelago located in the Caribbean Sea and comprised of three major island groups: the Greater Antilles (Hispaniola, Jamaica, Cuba, and Puerto Rico), the

Lesser Antilles (Windward and Leeward Islands), and the Bahamas (3,000 individual islands).

Two characteristics that define these island groups include: biota and geological history (Hedges

1996a).

More specifically, the West Indies is home to a major proportion of the Earth’s known terrestrial biota (Mittermeier et al. 2004). Many of the species that comprise this biota are not only endemic to the West Indies, but also to specific islands within the West Indies, and to specific localities within each island. Furthermore, the West Indies is an archipelago that has undergone many major geological changes and therefore has a complex geological history.

Together, these characteristics (biota and geological history) have facilitated the exploration of the historical biogeography of these islands (Hedges 2001).

Previous research in this area has focused on the testing of two biogeographic models: dispersal and vicariance. The dispersal model suggests that Caribbean animals arrived at their localities by way of the flight or flotsam. The vicariance model suggests that proto-Antillean landmasses connecting North and South America, fragmented during the late Cretaceous, and subsequently traveled eastward while carrying biota (Hedges 2006).

The dispersal theory has been supported by a wide and thorough range of evidence.

According to Hedges (2006) factors such as: taxonomy, phylogeny, the fossil record, paleogeography, water currents, divergence time estimates from molecular clocks, and many more, provide strong evidence that overwater dispersal was the mechanism for most West Indian

terrestrial biota. While no model has been definitively discounted, a strong trend in support of the dispersal theory has developed (Hedges 2006). In order to contribute further to this support,

scientists continue to expand the geological fossil record, make molecular divergence times

available, and determine the biogeography and phylogenetic relationships among endemic

terrestrial animals (Hedges 2001).

Diploglossidae

Diploglossidae is a family of Neotropical lizards (Vidal and Hedges 2009) whose

biogeography and phylogenetic relationships are not well established. The family is composed of

three genera; Celestus, Diploglossus, and Ophiodes, and approximately 46 species: 25 found in

the West Indies and 21 found in Central and South America (Savage et al. 2008). Despite previous work on the biogeography and phylogeny of these lizards, they remain poorly known.

There are three major questions: the generic composition of Diploglossidae (Ophiodes and

Celestus, Ophiodes and Diploglossus, or Ophiodes and both), the generic allocation of the two

tetradactyl diploglossid genera Sauresia and Wetmorena, and the definition and naming of all

Diploglossidae species.

Generic Composition of Diploglossidae

The most steadfast debate regarding the systematics of the lizard family Diploglossidae is

its generic composition. While the existence of the genus Ophiodes has never been questioned, it

remains unclear whether or not the remaining extant species compose one or two genera. Initial investigations regarding this uncertainty were based upon morphological characters alone, while later investigations utilized morphological characters, immunological distance data, and DNA sequence data (Hedges 2001).

The presence or the absence of the claw sheath was the primary morphological character used to determine the generic composition of Diploglossidae. Based upon the presence or the absence of the claw sheath respectively, most authors (Stejneger 1904, Barbour 1910, Burt and

Burt 1932, Stuart 1963, Peters and Donoso 1970, Meyer and Wilson 1973, and Savage and Lips

1993) recognized two genera: Diploglossus Wiegmann (1834) and Celestus Gray (1839). The validity of utilizing the presence or absence of the claw sheath, however, was questioned by other authors (Boulenger 1885, Dunn 1939, Underwood 1959, Campbell and Camarillo 1994, and Werler and Campbell 2004), who instead proposed the existence of one diploglossid genus,

Diploglossus.

Further morphological studies were based upon the developmental stage of the osteoderm canal system. Strahm and Schwartz (1977) utilized this new morphological character and ultimately recognized two distinct genera, Celestus and Diploglossus. The developmental stage of the osteoderm canal system was later shown to be a result of ontology (Wilson et al. 1986), however, and similar to the presence or absence of the claw sheath, was ultimately deemed a limited morphological character for the purposes of generic analysis.

More recent studies, however, have not relied solely on morphological characters. Based upon immunological distance data, DNA sequence data, and the presence or absence of nasal- rostral contact, two major genera of Diploglossidae were redefined: Diploglossus and Celestus

(Hass et al. 2001).

Despite advances in analyses (morphological vs. immunological and molecular), uncertainty remains regarding the generic composition of Diploglossidae. As a result, the most recent assessment of diploglossid biogeography and phylogeny, conducted by Savage et al.

(2008), has changed minimally since an earlier paper by Savage and Lips (1993). More

specifically, Savage et al. (2008) continue to distinguish and recognize two diploglossid genera,

Diploglossus and Celestus, based upon the presence or absence of the claw sheath respectively.

Generic Allocation of Sauresia and Wetmorena

In addition to resolving the generic composition of the lizard family Diploglossidae, the

generic allocation of the two endemic tetradactyl genera, Sauresia and Wetmorena, has also been highly debated. Utilizing the presence or the absence of the claw sheath, Savage and Lips (1993) supported the recognition of Sauresia and Wetmorena as two separate genera distinct from

Diploglossus. However, based upon immunological distance data and DNA sequence data,

Hedges (1996) synonymized the two tetradactyl diploglossid genera with Celestus. Macey et al.

(1999) did not include any West Indian Celestus in their study so their results were limited.

Contributing further to this debate, and based upon immunological distance data, Hass et al.

(2001) ultimately concluded that Sauresia and Wetmorena are more closely related to and nested within Celestus.

In addition, Hass et al. (2001) proposed the use of a different morphological character set to further examine this particular generic allocation. Based upon the presence or absence of nasal-rostral contact, Sauresia and Wetmorena were again synonymized with Celestus (Hass et al. 2001). However, Savage et al. (2008) placed more weight on the claw-sheath character than on the nasal-rostral character, and thus continued the debate. As a result, the two diploglossid tetradactyl genera Sauresia and Wetmorena are maintained as two separate genera, distinct from

Diploglossus.

Distinction and Naming of Species

In addition to determining the generic composition of the lizard family Diploglossidae, as

well as the generic allocation of the two endemic tetradactyl genera Sauresia and Wetmorena,

the recognition and naming of species within this family have also been topics of attention. The most recent assessment of the lizard family Diploglossidae by Savage et al. (2008), listed three diploglossid genera (Celestus, Diploglossus, and Ophiodes) and approximately 46 species: 25 found in the West Indies and 21 found in Central and South America (Table 1).

The results presented in this thesis, however, introduce many new diploglossid species.

And while numerous studies have been conducted to delineate and name diploglossid species, the most comprehensive were two studies done by Albert Schwartz (1964) (1965). He utilized morphological characteristics (patterning, coloration, and scalation) in conjunction with geographical distribution to delineate the diploglossid species stenurus, costatus, curtissi, and haetiana as well as their subspecies. The distinction among these diploglossid subspecies by way of geographical distribution was concordant with the results presented in this thesis, and will therefore be discussed below.

Celestus stenurus was determined to have four subspecies. The first subspecies was named Celestus stenurus stenurus and is distributed throughout the Tiburon Peninsula in Haiti.

The second subspecies was named Celestus stenurus weinlandi and is distributed throughout northern Haiti, starting in Gonaives, extending southeast to Plaine de Cul de Sac, and east to

Barahona in the Dominican Republic. The third subspecies was named Celestus stenurus rugosus and is distributed throughout northern Haiti as well as the northwestern Dominican

Republic. The fourth subspecies was named Celestus stenurus alloeides and is distributed throughout the Peninsula de Samana in the Dominican Republic (Schwartz and Henderson

1991).

Celestus costatus was determined to have eight subspecies, however, only four are relevant to the results presented in this thesis. The first subspecies was named Celestus costatus

costatus and is distributed throughout the Tiburon Peninsula of southwestern Haiti. The second

subspecies was named Celestus costatus oreistes and is distributed throughout southeastern

Haiti, and east into the Dominican Republic (Peninsula de Barahona). The third subspecies was

named Celestus costatus neiba and is distributed throughout the Sierra de Neiba in the

Dominican Republic. The fourth subspecies was named Celestus costatus melanchrous and is

distributed throughout north-central and eastern Haiti, as well as the Peninsula de Samana and

several inland localities (Hato Mayor, Monte Plata, San Cristobal, Peravia, Monsenor Nouel, and

La Vega) in the Dominican Republic (Schwartz and Henderson 1991).

Celestus curtissi was determined to have four subspecies, although only three are

pertinent to the results presented in this thesis. The first subspecies was named Celestus curtissi

curtissi and is distributed throughout the Cul-de-Sac Plain and St. Marc in Haiti, as well as the

Valle de Neiba of the Dominican Republic. The second subspecies was named Celestus curtissi

diastatus and is distributed in northwestern Haiti only. The third subspecies was named Celestus curtissi aporus and is distributed throughout the Dominican Republic including the Peninsula de

Barahona, north to Barahona, and west to the Dominican-Haitian border (Schwartz and

Henderson 1991).

Schwartz (1965) also named two new subspecies of Wetmorena haetiana. The first

subspecies was named Wetmorena haetiana surda and is distributed across the southern Haiti-

Dominican border. The second subspecies was named Wetmorena haetiana mylica and is located

throughout the Barahona Province of the Dominican Republic (Schwartz and Henderson 1991).

The importance of the two studies conducted by Albert Schwartz in 1964 and 1965 will be

discussed later in this thesis.

MATERIALS AND METHODS

All specimens used in this study came from the frozen tissue collection of S. Blair

Hedges. They were collected by hand at localities throughout the West Indies (see APPENDIX

A for specimen collection numbers and collection localities). A tissue sample from each

specimen was then either frozen in liquid nitrogen or conserved in 90% ethanol. All tissue

samples were stored at -80˚C in a laboratory freezer. Field and laboratory research were

approved by the Institutional Animal Care and Use Committee of Pennsylvania State University

(#17632).

DNA Extraction

In order to extract DNA from each tissue sample, a slightly modified version of the

QIAGEN DNeasy Tissue Extraction Protocol was used. First, approximately twenty five

milligrams of each tissue sample (liver, heart, or portion of tail) were cut into small pieces and placed into a 1.5 mL microcentrifuge tube containing ATL buffer (180 µL) and proteinase K (20

µL). The microcentrifuge tube was then vortexed for ten seconds and incubated at 56˚C until the

tissue sample was completely lysed (approximately 2.5 hours).

After lysing, the microcentrifuge tube was vortexed for 15 seconds. AL buffer (200 µL)

was added and the microcentrifuge tube was vortexed for ten seconds. Next, 98% ethanol (200

µL) was added and the microcentrifuge tube was again vortexed for ten seconds. The resulting

mixture was pipetted into the DNeasy mini spin column of a 2 mL collection tube. The collection

tube was centrifuged at 8,000 rpm for one minute. The flow through and collection tube were

discarded, and the remaining DNeasy mini spin column was placed into a new collection tube.

Buffer AW1 (500 µL) was added to the DNeasy mini spin column of the new collection tube and centrifuged at 8,000 rpm for one minute. The flow through and collection tube were

discarded, and the remaining DNeasy mini spin column was placed into a new collection tube.

Buffer AW2 (500 µL) was pipetted into the spin column of the new collection tube and

centrifuged at 14,000 rpm for three minutes. The flow through and collection tube were

discarded. To ensure that the membrane of the DNeasy mini spin column was dry, the spin

column was placed into a new collection tube, buffer AW2 (500 µL) was added, and the spin

column and collection tube were centrifuged at 14,000 rpm for one minute. The flow through

and collection tube were discarded.

The remaining DNeasy mini spin column was placed into a clean, labeled, 1.5 mL

microcentrifuge tube. Buffer AE (200 µL) was pipetted directly onto the DNeasy membrane

which was then allowed to incubate at room temperature for five minutes. In order to elute, the

DNeasy mini spin column and 1.5 mL microcentrifuge tube were centrifuged at 8,000 rpm for

one minute. The flow through was saved and placed into a laboratory refrigerator. The

remaining DNeasy spin column was placed into another clean, labeled, 1.5 mL microcentrifuge

tube. In order to further elute, buffer AE (200 µL) was added, the spin column and

microcentrifuge tube were incubated for five minutes at room temperature, and subsequently

centrifuged at 8,000 rpm for one minute. Completion of the modified QIAGEN DNeasy Tissue

Extraction Protocol resulted in two 1.5 mL microcentrifuge tubes containing extracted DNA

(200 µL in each) to be used for DNA amplification.

DNA Amplification

The 12S, 16S, and ND2 mitochondrial genes, and the RAG1 nuclear gene, were amplified using polymerase chain reaction (PCR). The 12S and 16S rRNA mitochondrial genes

were amplified in six overlapping segments using the following primers, respectively: 12L2,

12L5, 12L13, 16L10, 16L9, and 16L5 with 12H3, 16H2, 16H17, 16H1, 16H9, and 16H35s. The

ND2 mitochondrial gene was amplified in one segment using the following primers: ND2L2L

with SQ1L, Ang2R, Ang3R, and Ang4R. The RAG1 nuclear gene was amplified utilizing the

following primers: RAG1R1 and L2480 with H2920 and H2928 (see APPENDIX B for primer

sequences).

In order to amplify the 12S, 16S, and ND2 mitochondrial genes, the following mixture was pipetted into a 1.5 mL microcentrifuge tube: dH2O (23μl), mitochondrial dNTP (20 μl),

buffer (5 μl), L primer (1 μl), H primer (1 μl), and Taq DNA polymerase (0.2 μl). This mixture

(49 μl) was pipetted into a .2 mL PCR tube, followed by the sample (1 μl). The .2 mL PCR tube

was placed into a Genamp® PCR Systems 2400 and 2700 thermocycler. The thermocycler was

set to the following conditions: initiating at 94ºC for 150 seconds, denaturing at 94ºC for 30

seconds and 42-45 cycles, annealing at 45-50ºC for 45 seconds, and extending at 72ºC for 45

seconds.

In order to amplify the RAG1 nuclear gene, the following mixture was pipetted into a 1.5 mL microcentrifuge tube: dH2O (30.2 μl), DMSO (2.5 μl), nuclear dNTP (5 μl), buffer (5 μl), L

primer (1 μl), H primer (1 μl), and Taq DNA polymerase (0.5 μl). This mixture (45 μl) was then

pipetted into a .2 mL PCR tube, followed by the sample (5 μl). The .2 mL PCR tube was placed

into a Genamp® PCR Systems 2400 and 2700 thermocycler. The thermocycler was then set to

the following conditions: initiating at 94ºC for 150 seconds, denaturing at 94ºC for 30 seconds

and 42-45 cycles, annealing at 45-50ºC for 45 seconds, and extending at 72ºC for 45 seconds.

Gel Electrophoresis

Upon completion of each PCR, a high melting agarose gel was prepared. In order to do this, .6 grams of high melting agarose and 50 mL of TBE were mixed together in a beaker. The agarose/TBE mixture was then heated in the microwave for one minute. Ethidium bromide (4μl)

was added to the beaker and mixed. The contents of the beaker were then poured into a gel box tray. The gel solidified and was subsequently used to visualize the PCR product.

Each resulting PCR mixture (5 μl) was mixed with 6x dye (1.5 μl) and loaded into a well in the high melting agarose gel. A 1000 base pair DNA ladder, negative, and positive control were also loaded into the gel. The gel box was then connected to the gel electrophoresis machine, and the machine was turned on and set at 142 volts. Amplified DNA fragments were then separated based on size.

After approximately thirty minutes, the gel was viewed under an ultraviolet light. Faint and smeared bands were re-amplified utilizing modified PCR conditions. Sharp and bright bands were purified and sequenced.

DNA Purification

Gel purification was utilized to isolate the sharp and bright DNA fragments from each

PCR product. First, a low EEO agarose gel was prepared. In order to do this, .60 grams of low melting agarose and 50 mL of TAE were mixed in a beaker and heated in a microwave for one minute and thirty seconds. Ethidium bromide (5 μl) was then added, and the mixture was poured into a gel purification gel box tray and allowed to solidify. The remaining PCR product (49 μl) was then mixed with 2x dye (3 μl) and loaded into the prepared gel. The gel box was then connected to the gel electrophoresis machine, and the machine was turned on and set at 142 volts.

After approximately 30 minutes of electrophoresis, the bands were visualized under ultraviolet light, cut out of the gel, and place into a Montage DNA Gel Extraction tube. In order to remove contaminants from each band of DNA fragments, each Montage DNA Gel Extraction tube was centrifuged for ten minutes at 8,000 rpm. The filter of each tube was discarded, and the

remaining tubes were stored in a laboratory freezer at -4ºC until DNA sequencing was

conducted.

DNA Sequencing

In order to sequence the gel purified DNA, a ThermoGrid PCR plate was obtained, and

two wells were used for each purified DNA sample. Purified DNA (2 μl) was pipetted into

each well, and diluted L primer (2 μl) was pipetted into one well and diluted H or R primer (2

μl) was pipetted into the other. Filled ThermoGrid PCR plates were taken to the Nucleic Acid

Facility in University Park, PA. Forward and reverse directions of each fragment were

sequenced. The resulting sequence was edited and aligned using Mega v5.0 software (Tamura

et al. 2010).

Phylogenetic Analysis

Mitochondrial (12S, 16S, ND2) and nuclear (RAG1) sequence data were evaluated,

aligned, and edited using Mega v5.0 (Tamura et al. 2010). The aligned and edited sequence data were then analyzed using two methods of phylogenetic analysis: Neighbor Joining (NJ) and ML. One outgroup taxa was used (Ophisaurus apodus) in addition to the 145 diploglossid specimens.

Mega v5.0 (Tamura et al. 2010) and the default Neighbor Joining parameters were used

to generate the initial topology in the form of a Neighbor Joining tree. Mega v.5.0 was utilized in

order to determine the most likely phylogeny in the form of a Maximum Likelihood (ML) tree.

The following parameters were used: general time reversible (GTR) model with a proportion of

invariable sites and variable rates across sites simulated by gamma distribution (GTR+I+G).

RESULTS AND DISCUSSION

Two phylogenetic trees were generated with the ML analysis. One ML phylogenetic tree

was generated using sequence data obtained for four genes (12S, 16S, ND2, RAG1) totaling

3,736 base pairs and 21 diploglossid specimens, and one outgroup. An additional ML

phylogenetic tree was generated using sequence data obtained for the mitochondrial gene ND2

(328 base pairs), 145 diploglossid specimens, and one outgroup. Bootstrap values were also

calculated for every major node in each tree. Bootstrap values of 95% or greater were considered

significant.

Despite some variation in the number of specimens and the amount of sequence data used to generate each ML tree, the two tree topologies were fairly consistent in the distinction between genera and lineages, as well as the groupings of specimens belonging to each taxon.

Furthermore, while bootstrap values were not significant at every node within both trees, new

and distinct groupings of specimens were found and the recognition of some new subspecies was

suggested by the data. Finally, support for the dispersal model was evident due to the genetic

separation of Hispaniolan and Jamaican Celestus as well as Puerto Rican and Cuban

Diploglossus.

Figure 1 represents the tree topology and bootstrap values produced by Mega v5.0 using the ML analysis and a data set composed of 328 base pairs of ND2 for 145 Diploglossidae specimens, and one outgroup. Figure 2 represents the tree topology and bootstrap values produced by Mega v5.0 using the ML analysis and a data set composed of 3,376 base pairs of

ND2, 12S, 16S, and RAG1 combined, for 21 Diploglossidae specimens and one outgroup. Both

trees will be dissected in terms of the four debates discussed within the introduction:

biogeography of diploglossids, generic composition of Diploglossidae, generic allocation of

Sauresia and Wetmorena, and distinction and naming of species.

The first nine diploglossid specimens in Figure 1 comprise the genus, species, and subspecies Celestus costatus neiba respectively. Schwartz (1964) described Celestus costatus neiba based upon its morphological characters and localization to the Sierra de Neiba in the

Dominican Republic. The nine specimens of Celestus costatus neiba, are not only found within the Dominican Republic, but are also from localities (Cacique Enriquillo, Rosa de La Pierda,

Apolinar Pelodroma, Los Pinos, and La Descubierta) nearby or within the Sierra de Neiba. As a result, they have been identified here as Celestus costatus neiba.

The next diploglossid specimen comprises the genus, species, and subspecies Celestus curtissi curtissi respectively. Schwartz (1964) determined that the range of Celestus curtissi curtissi extends from northwest Haiti through the central and southern areas of the Dominican

Republic. The Celestus curtissi curtissi specimen is in fact located within this range, as it is found along the Haitian-Dominican border (La Descubierta). As a result, this specimen has been identified here as Celestus curtissi curtissi.

Schwartz (1964) determined that Celestus curtissi diastatus is distributed in northwestern

Haiti. The two specimens that comprise this group, in this analysis, are distributed in northwestern Haiti (Marc St. Nicolas), and have therefore been identified as Celestus curtissi diastatus.

The next cluster of five specimens comprises Celestus marcanoi. These two specimens are located in northern/central Dominican Republic (Valle de Bao), and thus are consistent with the current assignment of this species (Savage et al. 2008).

Beneath Celestus marcanoi in the tree is Celestus curtissi aporus comprised of 13 specimens. Schwartz (1964) originally defined Celestus curtissi aporus, as ranging from southwestern Dominican Republic (Peninsula de Barahona) all the way west to the Dominican-

Haiti border. The 13 specimens constituting this subspecies are found within this distribution

(Los Arroyos, Juancho, La Descubierta, Bucan Detwi, Barahona). As a result, this group of specimens has been identified here as Celestus curtissi aporus.

The next four specimens comprise Celestus costatus costatus, a subspecies originally named based upon its distribution throughout southwestern Haiti (Tiburon Peninsula). These specimens are in fact located along the Tiburon Peninsula (Castillion, Beaumont, Abricots). Due to locality concordance these specimens were designated as Celestus costatus costatus.

Celestus costatus oreistes is distributed from southeastern Haiti all the way to the eastern side of the Peninsula de Barahona in the Dominican Republic (Schwartz 1964). The two specimens that comprise this group are located within this distribution (Jacmel, Puerto

Escondido), and they were therefore named Celestus costatus oreistes.

The next 29 specimens comprise a group composed of the following Celestus species:

Celestus hewardii, Celestus crusculus, Celestus duquesneyi, and Celestus barbouri. All of these specimens are from Jamaica and form a separate and nested group within Hispaniolan Celestus.

The location of this group indicates a potential colonization of Jamaican Celestus from

Hispaniola, probably by way of the unidirectional West Indies current flow, and in support of the dispersal theory (Hedges 2006). In addition, the two Celestus barbouri specimens form a strongly supported group (99%), and it is clear that some potentially new Jamaican Celestus species exist based upon the position of various crusculus specimens in the tree, some clustering

(distantly) with other species. It is, however, difficult to make any firm conclusions here because

museum specimens will need to be examined and possibly additional genes will need to be

sequenced.

The next six specimens comprise two Celestus species from unique localities. The first

specimen is Celestus badius from Navassa Island, between Haiti and Jamaica. The next five specimens are Celestus maculatus from the Cayman Brac in the Cayman Islands. Both are

distinct and valid Celestus species and are most closely related to species of Celestus from

Hispaniola, consistent with the westward direction of ocean current (Hedges 2006).

I assign the next 12 specimens to the subspecies Celestus stenurus rugosus. Schwartz

(1964) defined the distribution of this subspecies as occurring throughout northern Haiti as far east as Llanos de Azua, but not including the Peninsula de Samana in the Dominican Republic.

Schwartz (1964) also defined an adjacent subspecies, Celestus stenurus alloeides as occuring throughout the Peninsula de Samana. The twelve Celestus specimens in question form a single group showing almost no genetic differences and spanning localities consistent with both

Celestus stenurus rugosus and Celestus stenurus alloeides (Las Galeras, Nagua, El Valle,

Tenares, San Francisco de Macoris, Sabana de la Mar). As a result, these specimens have been reassigned here to the first named subspecies Celestus stenurus rugosus.

The next group is comprised of four specimens named Celestus stenurus stenurus, which was originally named due to its distribution throughout the Tiburon Peninsula as well as within the vicinity of Port-au-Prince and Ile-a-Vache (Schwartz 1964). The four specimens that comprise this group are also distributed in this area (Rampe des Lions and Boios Sec, Ile-a-

Vache), and as a result were named Celestus stenurus stenurus.

The next nine specimens form Celestus stenurus weinlandi. Schwartz (1964) named

Celestus stenurus weinlandi based upon its distribution throughout northern Haiti, starting in

Gonaives, extending southeast to Plaine de Cul de Sac, and east to Barahona in the Dominican

Republic. Four of the nine specimens that comprise Celestus stenurus weinlandi are distributed along the Haitian side of the Haiti-Dominican border (Thomaseau, Soliette), while two of the specimens are distributed along the Dominican side of the Haiti-Dominican border (La Zurza,

Ouest), and two are located as far east as Conoa and El Azul in the Dominican Republic. As a result of locality, this group was named Celestus stenurus weinlandi.

The next three specimens comprise Celestus agasepsoides (99% BS). This highly supported species was originally named Sauresia agasepsoides (Thomas 1971), and remained

Sauresia agasepsoides in the most recent reassessment of diploglossid systematics (Savage et al.

2008). This group therefore is part of the debate regarding the generic allocation of the

tetradactyl genera Sauresia and Wetmorena. Based upon the position of this species within

Figure 1, it is clear that agasepsoides is more closely related to Celestus than Diploglossus, and

supports the conclusions of Hedges (1996) and Hass et al. (2001).

The two groups below Celestus agasepsoides include Celestus haetianus mylicus and

Celestus haetianus surdus. Schwartz (1965) defined the first subspecies as occuring throughout

the Barahona Province of the Dominican Republic and the second as being distributed across the

southern Haiti-Dominican border. The specimens in the two aforementioned genetic groups in

Figure 1 are consistent with the distributions of the two subspecies. It is also clear that these

specimens, once placed in Wetmorena, are genetically more closely related to Celestus than

Diploglossus, as found earlier by Hass et al. (2001).

Beneath Celestus haetianus mylicus and Celestus haetianus surdus is a group of 19

specimens that represent Celestus sepsoides. This species was originally named Sauresia

sepsoides (Gray 1852), and in the most recent reassessment of Diploglossidae, remained

Sauresia sepsoides (Savage et al. 2008). As a result, the recognized name, Celestus sepsoides,

bears on the debate regarding the generic allocation of the endemic tetradacyl genera Sauresia

and Wetmorena. Based upon the position of this species within Figure 1 and concordant with

Hedges (1996) and Hass et al. (2001) it is clear that Celestus sepsoides is more closely related to

Celestus than Diploglossus.

The next five specimens compose the final two West Indian Celestus species: warreni

(99% BS) and darlingtoni (99%). The Celestus warreni specimens are localized to northeastern

Dominican Republic (Puerto Plata) while Celestus darlingtoni specimens are localized to the

Dominican-Haiti border (Independencia). Based upon geographical isolation and strong

bootstrap values, there is no question that these are valid and distinct species.

The next eight specimens introduce and comprise three West Indian Diploglossus species

(98% BS). Diploglossus pleii is localized on the West Indian island of Puerto Rico and forms a

highly supported (99% BS) and distinct species. Diploglossus garridoi and D. nigropunctatus

are located throughout the West Indian island of Cuba and form two distinct species. The most

important part in identifying and distinguishing these three West Indian Diploglossus species is

its implications with regard to the debate surrounding the generic composition of the lizard family Diploglossidae. Based upon Figure 1 and in concordance with the most recent reassessment of Diploglossidae (Savage et al. 2008), it is clear that Celestus and Diploglossus are in fact separate genera. Another important consideration with regard to this group is biogeography. While West Indian Celestus and Diploglossus are clearly localized to specific islands, the distribution of Diploglossus, like the distribution Celestus, is concordant with the

dispersal theory in being found on multiple islands. More specifically, it is possible that Puerto

Rican Diploglossus radiated and colonized Cuba by way of flotsam and the unidirectional West

Indian current flow (Hedges 2006).

The final three specimens introduce and comprise the mainland Celestus and

Diploglossus. The three species that comprise this group are Celestus cyanochloris, Celestus

enneagrammus, and Diploglossus bilobatus, and are distributed in Mexico and Costa Rica

respectively. While it is questionable as to why Celestus enneagrammus from Mexico and

Diploglossus bilobatus from Costa Rica are more closely related than Celestus enneagrammus

and Celestus cyanochloris, it is important to note and conclude that mainland Celestus and

Diploglossus are genetically distinct lineages from West Indian Celestus and Diploglossus. This result will require a future reassessment of the generic-level taxonomy of the lizard family

Diploglossidae.

Table 1. Most recent reassessment of phylogeny and taxonomy of the lizard family Diploglossidae. This reassessment was based upon morphology and geography (Savage et al. 2008).

Character states and geographic distribution of recent diploglossine lizards 1 1 FN + 1 CLAWS 1 FN + 2 FN/PF CLAWS EXPOSED 2 PF FN/PF SHEATHED PF Celestus Diploglossus CA adercus +, c atitlanensis montanus +, j CA +, j CA WI striatus -, ? CA bilobatus +, u, j CA/NSA monotropis +, s, c montisilvestris -, M ingridae +, c CA s, c SA fasciatus u, c

M/CA enneagrammus +, c, *** SA lessonae +, s, c MO montisserrati +, s, c, * CA bivittatus +, j CA cyanochloris +, c C garridoi +, s, cf MA millepunctatus +, s, c nigropunctatus CA hylaius +, j C +, s, cf M legnotus +, c PR pleii +, s, c CA orobius +, j Sauresia CA rozellae +, j agasepsoides -, CA scansorius +, j H s, c H anelpistus -, c H sepsoides -, s, c H costatus -, c Wetmorena H darlingtoni -, c,** H haetiana -, s, c H macrotus -, c NO CLAWS H marcanoi -, c H stenurus -, c Ophiodes H warreni -, c SSA intermedius -, c NI badius +, c SSA striatus -, c J barbouri -, c SSA vertebralis -, c JCI cusculus -, c SSA yacupoi -, c J curtissi -, c J duquesneyi -, c J fowleri -, c J hewardi -, c J microblepharis -, c J occiduus -, c C= Cuba, CA = Central America, CI = Cayman Islands, H = Hispaniola, J = Jamaica, M = Mexico, MA = I. Malpelo, MO = I. Montserrat, N = I. Navassa, NSA = Northern South America, SA = South America, SSA = Southern South America, WI = West Indies. Nasal-rostral contact: + = present, – = absent; postoculars-suboculars: c = continuous, j = juxtaposed; postoculars continuous with large supralabial = cf; * = frontonasal and prefrontals partially fused; ** = rarely divided into frontonasal and prefrontals; *** = rarely divided into 2 frontonasals or frontonasal and prefrontals; u = ungual overlap, s = subungual overlap.

Figure 1. ML tree created using ND2 mtDNA sequence data. Bootstrap values are displayed at nodes.

Figure 2. ML tree generated using 12S, 16S, ND2, and RAG1 sequence data. Bootstrap values are displayed at nodes.

Figure 3. West Indian Water Currents. Water currents suggest movement of lizards via dispersal.

ACKNOWLEDGEMENTS

I would first and foremost like to thank Dr. S. Blair Hedges. Dr. Hedges provided me with the unique opportunity to work individually on an ongoing and complex project during my freshman year at Penn State. This unique opportunity not only taught me many valuable skills, but also opened innumerable doors for myself as a student. Without the provided opportunity, guidance, and encouragement of Dr. Hedges, I probably would not have had the opportunity to learn, thrive on, explore, and excel in biological research, nor would I be a coveted Schreyer

Scholar. For all of these reasons, I will forever be thankful for Dr. Hedges.

I would also like to thank Dr. Carla Ann Hass. Dr. Hass provided unwavering knowledge, guidance, and patience, when I first began my research project and throughout my four years working on it. She taught me the meaning and importance of precision, detail, and utmost standards with regards to conducting research, and these are qualities I will continue to apply to many aspects of my life, and will forever be thankful for.

Finally, I would like to thank Matthew Heinicke, Caitlin Conn, and Kelly Lipp. Matthew

Heinicke provided answers to every single one of my questions, no matter how simple or complex they were. Caitlin Conn and Kelly Lipp have provided constant support and friendship throughout my research experience, as well as aided me in the brainstorming and troubleshooting necessary for the progression of my research project.

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APPENDIX A. SPECIMEN LOCALITIES. Sample localities and specimen numbers of Diploglossidae used in this study. SBH sample numbers indicate those in the collection of S. Blair Hedges at Pennsylvania State University.

CAYMAND ISLANDS

SBH Sample Species Province Locality Number Celestus maculatus Cayman Brac West End (area surrounding Tiara Beach Hotel) 266550 Celestus maculates Cayman Brac West End (area surrounding Tiara Beach Hotel) 266551 Celestus maculatus Cayman Brac West End (area surrounding Tiara Beach Hotel) 266552 Celestus maculatus Cayman Brac West End (area surrounding Tiara Beach Hotel) 266553 Celestus maculatus Cayman Brac Hawkbill Bay, .7 km E of 266527

COSTA RICA SBH Sample Species Province Locality Number Celestus cyanochloris Cartago Refugio Nacional Tapanti 194930 Diploglossus bilobatus Cartago Moravia 194940

HISPANIOLA

SBH Sample Species Province Locality Number Celestus agasepsoides Barahona Canoa, 0.3 km S, 13.5 km E of 160191 Celestus agasepsoides Pedernales Bucan Detwi 194829 Celestus agasepsoides Pedernales Bucan Detwi 194830 Celestus c. aporus Independencia La Descubierta, 5.1 km NW of 102609 Celestus c. aporus Independencia La Descubierta, 5.1 km NW of 102610 Celestus c. aporus Independencia La Descubierta, 5.1 km NW of 102611 Celestus c. aporus Barahona Barahona, 4.5 km S, 4.0 mi W of 102706 Celestus c. aporus Pedernales Juancho, SW of Enriquillo 102707 Celestus c. aporus Pedernales Juancho, SW of Enriquillo 102709 Celestus c. aporus Barahona Barahona, 4.5 km S, 2.7 km W of 102716 Celestus c. aporus Pedernales Los Arroyos 102915 Celestus c. aporus Barahona Barahona, 11.3 km S of 102930 Celestus c. aporus Pedernales Bucan Detwi 194831 Celestus c. aporus Pedernales Bucan Detwi 194832 Celestus c. aporus Pedernales Bucan Detwi 194833 Celestus barbouri Barahona Tejunde 13 km SSW La Guazara 160146 Celestus barbouri Barahona Tejunde 13 km SSW La Guazara 160147 Celestus c. costatus Grand' Anse Castillion, 8.0 km S, 0.3 km E of Marche Leon 103085 Celestus c. costatus Grand'Anse Castillion, 3 km SW of Marche Leon 104113 Celestus c. costatus Grand' Anse Abricots 192054 Celestus c. costatus Grand' Anse Beaumont, 17.0 km S of 192260 Celestus c. costatus Dept. du Sud Caye Mechel 192261 Celestus c. curtissi Independencia La Descubierta, 5.1 km NW 194503 Celestus darlingtoni Independencia Cacique Enriquillo, 9 km N of 161686 Celestus darlingtoni Independencia Cacique Enriquillo, 9 km N of 161687 Celestus darlingtoni Independencia Cacique Enriquillo, 9 km N of 161688 Celestus c. diastus Dept. du Nord’Ouest Mole St. Nicolas 194553 Celestus c. diastus Dept. du Nord’Ouest Mole St. Nicolas 194580 Celestus marcanoi Santiago Valle de Bao; -70.98, 19.05 193279 Celestus marcanoi Santiago Valle de Bao; -70.98, 19.05 193280

Celestus marcanoi Santiago Valle de Bao; -70.98, 19.05 193286 Celestus marcanoi Santiago La Lagunas 193309 Celestus marcanoi Santiago Loma de Baito 193310 Celestus h. mylicus Barahona Cabral, 15.3 km S, 6.7 km E; -71.22, 18.10 102546 Celestus h. mylicus Barahona Cabral, 15.3 km S, 6.7 km E; -71.22, 18.10 102547 Celestus h. mylicus Barahona Cabral, 15.3 km S, 6.7 km E; -71.22, 18.10 102550 Celestus h. mylicus Barahona Cabral, 15.3 km S, 6.7 km E; -71.22, 18.10 160201 Celestus c. neiba Independencia La Descubierta, 6.2 km N, 4.0 km W 102922 Celestus c. neiba Elisa Pina Los Pinos, ca. 24 km N of 102923 Celestus c. neiba Elisa Pina Los Pinos, ca. 24 km N of 102924 Celestus c. neiba Elisa Pina Los Pinos, ca. 24 km N of 102925 Celestus c. neiba Elisa Pina Los Pinos, ca. 24 km N of 161498 Celestus c. neiba L’Artibonite Ca Soleil, 11.8 km W of 192428 Celestus c. neiba Baoruco Apolinar Pelodroma, ca. 5 km N of 194362 Celestus c. neiba Baoruco Apolinar Pelodroma, ca. 5 km N of 194363 Celestus c. neiba Elisa Pina 0.6 km NE Rosa de la Pierda 266321 Celestus c. neiba Independencia La Zurza 266683 Celestus c. oreistes Sud’Est Jacmel, 9.5 km E of 104408 Celestus c. oreistes Independencia Puerto Escondido, 23.9 km SE of 192480 Celestus sepsoides Hato Mayor Sabana De La Mar, 9.5 km W of 102369 Celestus sepsoides Grand’ Anse Pestel, 5.0 km S of 191850 Celestus sepsoides Grand’ Anse Marche Leon, 8.0 km S of 191913 Celestus sepsoides La Altagracia La Zanga, 7.5 km W of 192469 Celestus sepsoides Los Tabucos Tenares, 8.8 km N, thence .5 km W 193201 Celestus sepsoides Sud’ Est Jacmel, 9.5 km E of 266748 Celestus sepsoides Sud’ Est Jacmel, 11.4 km E, 1.0 km N at Ravine Normandy 266749 Celestus s. stenurus Grand Anse Rampe des Lions and Boios Sec 103900 Celestus s. stenurus Grand Anse Rampe des Lions and Boios Sec 103901 Celestus s. stenurus Grand Anse Rampe des Lions and Boios Sec 103902 Celestus s. stenurus Dept. de Sud lle a Vache 267494 Celestus h. surdus Sud’ Est Gros Cheval, ca. 15 km W of 104418 Celestus h. surdus Sud’ Est Gros Cheval, ca. 15 km W of 104419 Celestus h. surdus Independencia Puerto Escondido, 23.9 km SE of 192486 Celestus rugosus El Seibo Sabana de La Mar, 1.7 km W of 101247 Celestus s. rugosus El Seibo Sabana de La Mar, 1.7 km W of 101248 Celestus s. rugosus Hato Mayor El Valle, ca. 15 km W of 102371 Celestus s. rugosus Samana Las Galeras, 6 km SSW of 103075 Celestus s. rugosus Maria Trinidad Sanchez Nagua, 4 km SE of 103083 Celestus s. rugosus Maria Trinidad Sanchez Nagua, 4 km SE of 103084 Celestus s. rugosus Duarte San Francisco de Macoris, 10.1 km NE of 193191 Celestus s. rugosus Duarte San Francisco de Macoris, 10.1 km NE of 193192 Celestus s. rugosus Los Tabucos Tenares, 8.8 km N of 193197 Celestus s. rugosus Los Tabucos Tenares 8.8 km N of 193198 Celestus s. rugosus Los Tabucos Tenares, 14.0 km N of 193216 Celestus s. rugosus Los Tabucos Tenares 14.0 km N of 193217 Celestus sepsoides Monte Plata Majagual, 4.3 km N of 193115 Celestus sepsoides Monte Plata Majagual, 4.3 km N of 193116 Celestus sepsoides Monte Plata Majagual, 4.3 km N of 193117 Celestus sepsoides Monte Plata Majagual, 4.3 km N of 193118 Celestus sepsoides Los Tabucos Tenares, 8.8 km N of 193202 Celestus sepsoides Los Tabucos Tenares, 8.8 km N of 193203 Celestus sepsoides Los Tabucos Tenares, 8.8 km N of 193204 Celestus sepsoides Sanchez Ramirez Cotui, 8.6 km N of 193040 Celestus sepsoides Sanchez Ramirez Cotui, 8.6 km N of 193041 Celestus sepsoides Sanchez Ramirez Cotui, 8.6 km N of 193042 Celestus sepsoides Dept. de la Grand’ Anse Pestel, 11.8 km S of 191851 Celestus sepsoides Dept. de la Grand’ Anse Marche Leon, 8.0 km S of 191914 Celestus sepsoides Dept. de la Grand’ Anse Marche Leon, 8.0 km S of 191915 Celestus sepsoides Dept. de la Grand’ Anse Baraderes, 8.0 km SSW of 192312 Celestus sepsoides Dept. de la Grand’ Anse Baraderes, 8.0 km SSW of 192313 Celestus warreni N/A 172914 Celestus warreni N/A 194521

Celestus s. weinlandi Barahona Canoa, 16 km ESE of 102712 Celestus s. weinlandi I’Ouest Soliette 103607 Celestus s. weinlandi Dept. De L’Ouest Thomaseau, 18.7 km E of 191591 Celestus s. weinlandi Dept. De L’Ouest Thommaseau, 18.7 km E of 191622 Celestus s. weinlandi Dept. De L’Ouest Thommaseau, 18.7 km E of 192405 Celestus s. weinlandi San Juan El Azul, NNE 1.6 mi. 266286 Celestus s. weinlandi Independencia La Zurza 266682 Celestus s. weinlandi Independencia La Zurza 266684 Celestus s. weinlandi Independencia La Zurza 266685

JAMAICA

SBH Sample Species Province Locality Number Celestus barbouri Trelawny Quick Step, vicinity of 161120 Celestus barbouri Trelawny Windsor, 0.5 km N of 190678 Celestus crusculus St. Catherine Ewarton, 9.0 km W 101571 Celestus crusculus Hanover: Content Content, 3.2 km SE 101572 Celestus crusculus Trelawny Duncans, 0.3 km W at jct with Silver Sands Road 172442 Celestus crusculus Westmoreland Town Head, 5.3 km N 101554 Celestus crusculus St. Elizabeth Knoxwood 103448 Celestus crusculus St. Elizabeth Knoxwood 103449 Celestus crusculus Clarendon Jackson Bay, ca 1.6 km ESE 103460 Celestus crusculus Clarendon Jackson's Bay (on beach at hunting club) 103586 Celestus crusculus Trelawny Duncans, 0.3 km W (jct. with Silver Sands road) 161123 Celestus crusculus St. Mary Salt Gut, vicinity of Boscobel Airport (east side) 161124 Celestus crusculus St. Mary Oracabessa, 6.2 km W of 171439 Celestus crusculus St. Mary Port Maria, ca. 6.4 km S of 171601 Celestus crusculus St. Mary Port Maria, ca. 6.4 km S of 171603 Celestus crusculus St. Mary Port Maria, ca. 6.4 km S of 171604 Celestus crusculus St. Mary Port Maria, ca. 6.4 km S of 171605 Celestus crusculus St. Mary Oracabessa, 6.2 km W of 171606 Celestus crusculus St. Catherine Braeton, 5.6 km SW in Hellshire Hills at Hellshire Beach 171607 Celestus crusculus St. Mary Oracabessa, 6.2 km W 172438 Celestus crusculus Westmoreland Old Hope, 7.0 km WSW of 172441 Celestus crusculus Portland Section, 1.3 km WSW on road to Hardwar Gap 172465 Celestus crusculus St. Catherine Ewarton, 9.0 km W 101570 Celestus crusculus Hanover Content, 3.2 km SE 101573 Celestus crusculus St. Mary Oracabessa, ca. 1.6 km S (on road to Jacks River) 101615 Celestus crusculus Trelawny Duncans, 0.3 km W at jct with Silver Sands Road 172439 Celestus crusculus Westmoreland Old Hope, 4.5 km W at Little Bay 172440

NAVASSA ISLAND

SBH Sample Species Province Locality Number Celestus badius N/A N/A 194964

PUERTO RICO

SBH Sample Species Province Locality Number Diploglossus pleii Reserva Forestal Rio Abajo 191574 Diploglossus pleii Reserva Forestal Rio Abajo 191575

APPENDIX B. 12S, 16S, ND2, RAG1 PRIMERS.

12S, 16S

Forward 5' 3’ 12L2 AAAGCAWRGCACTGAARATGCTWAGATG 12L5 GATTAGATACCCCACTATGC 12L13 AAAGAAGAGGAAAGTCGTAACATGGTA 16L10 AGTGGGCCTAAAAGCAGCCA 16L9 CGCCTGTTTATCAAAAACAT 16L5 ACTGATCTT

Reverse 5’ 3’ CTAGAGGAGCCTGTTCTYTAATCGATAKKCCRC 12H3 G 16H21 GTACCTHTTGCATCATGGTYYAGCDAG 16H17 GCWRRRGGRKATGTTTTTGGTAAACA 16H1 CCTACGTGATCTGAGTTAGACCGGAG 16H9 CCGGTCTGAACTCAGATCACGT 16H35 RRGRKYTTAGGCCTTTTGCAT

ND2 Forward 5’ 3’ L2L TCCCAAAATGATTAACCMTAGAAGAA SQ1L AAGCTHTYGGGCCCATACCCCRAAAATG

Reverse 5’ 3’ Ang2R TCCCAAAATGATTAACCMTAGAAGAA Ang3R TAACCGGGTTYATCCCAAAATGATT Ang4R CTTGTARTACYTCTGGRAGTCAGAAGTG

RAG1 Forward 5’ 3’

RAG1R1 GACCAGTTCACAAACTGCTTCCAC

Reverse 5’ 3’

L2480 TGCACTGTGACATTGGCAA H2920 GCCATTCATTTTYCGAA

H2928 GACTGCYTGGCATTCATTTT

APPENDIX C. 12S, 16S rRNA mtDNA SEQUENCE DATA.

C.agasepsoides_160191_Canoa ACACCCCAGT GAAAATGCCC AAAA---CAC --AACTAACA -- GGA [ 45] C.curtissi_aporus_102610_La_Descubierta ...... ---... TA...G.... -- ... [ 45] C.badius_194964_Navassa_Island ...... ---... -A...... -- ... [ 45] C.barbori_161120_Quick_Step ...... ---... -A...A.... -- ... [ 45] C.costatus_costatus_192054_Abricots G...... ---... TA...A.... -- ... [ 45] C.darlingtoni_161687_Cacique_Enriquillo ....T...... ----... -A...A.... -- ... [ 45] C.maculatus_266527_Cayman_Brac ..G...... ---... -A..T..... -- ... [ 45] C.marcanoi_193286_Valle_de_Bao ...... - ....---... TA...G.... -- ... [ 45] C.costatus_neiba_192428_Ca_Soleil ...... ---... TA...G.... -- ... [ 45] C.costatus_oriestes_104408_Jacmel ...... ---... TA...A.... -- ... [ 45] C.stenurus_rugosus_103083_Nagua ..G..A...... ---... -A.GT..... -- ... [ 45] C.sepsoides_102369_Sabana_de_la_Mar ...... ---... --...G.C.. -- ... [ 45] C.warreni_172914_Hispaniola ...... ---... -A...A.... -- ... [ 45] C.warreni_194521_Puerto_Plata ...... ---... -A...A.... -- ... [ 45] D.nigropuntatus_191015_Cuba G...A...... T----... -AT.AAC.T. -- ... [ 45] D.nigropunctatus_191320_Cuba G...A...... T----... -AT.AAC.T. -- ... [ 45] D.nigropunctatus_191542_Cuba ....A...... T----... -AT.AAC.T. -- ... [ 45] D.pleii_191574_Puerto_Rico ------... [ 45] C.crusculus_101572_Content ...T...... ---... -A...... -- ... [ 45] C.crusculus_172442_Duncans ...... ---... -A...... -- ... [ 45] C.cyanochloris_194930_Costa_Rica ...AA...... T----TC. TA.CAC...C -- ... [ 45] C.enneagrammus_191044_Mexico_23 ...AT...... T----TTT TA.CA-G..C -- ... [ 45]

O.apodus_194938 ....A...... T...CAATCT TA.CAA.... AA... [ 45] C.agasepsoides_160191_Canoa GCGGGTATCA GGCAC---AC AC---TTTTA GCCCAAAACA CCTTG [ 90] C.curtissi_aporus_102610_La_Descubierta ..C...... ---.. ..AAGCC...... TG...... [ 90] C.badius_194964_Navassa_Island ..C...... ---.. ----T..C...... T...... [ 90] C.barbori_161120_Quick_Step ..T...... ---.. ..AAT...... T...... [ 90] C.costatus_costatus_192054_Abricots ..C...... ---.. .TAA-....G .....TG...... [ 90] C.darlingtoni_161687_Cacique_Enriquillo ..C...... ---.. ..AACC...... T...... [ 90] C.maculatus_266527_Cayman_Brac ..C...... G.---.. ----T...... T...... [ 90] C.marcanoi_193286_Valle_de_Bao ..C...... ---.. ..A--.C...... TG...... [ 90] C.costatus_neiba_192428_Ca_Soleil ..C...... ---.. ..A--.C..G .....TG...... [ 90] C.costatus_oriestes_104408_Jacmel ..C...... ---.. ..AA-...... TG...... [ 90] C.stenurus_rugosus_103083_Nagua ..C...... G.---.. ----T....- .....T...... [ 90] C.sepsoides_102369_Sabana_de_la_Mar ..C...... ---.. .A---..C...... T...... [ 90] C.warreni_172914_Hispaniola ..C...... ---.. ..AAT-...... T...... [ 90] C.warreni_194521_Puerto_Plata ..C...... ---.. ..AAT-...... T...... [ 90] D.nigropuntatus_191015_Cuba ..A.AC...... ---.A ..CACC-A.. ...A.C...G T.... [ 90] D.nigropunctatus_191320_Cuba ..A.AC...... ---.A ..CACC-A.. ...A.C...G T.... [ 90] D.nigropunctatus_191542_Cuba ..A.AC...... ---.A ..CACC.A.. ...A.C...G T.... [ 90] D.pleii_191574_Puerto_Rico ..-.....-...... ---.T ..CACA-A.. ...A.T.-...... [ 90] C.crusculus_101572_Content ..C...... ---.. ..AAT...... T.T...... [ 90] C.crusculus_172442_Duncans ..C...... ---.. --AAT...... T...... [ 90] C.cyanochloris_194930_Costa_Rica ..A...... AAC.. ..AACC...G ...A.T...... [ 90] C.enneagrammus_191044_Mexico_23 ..A...... AAC.. TATACCC..G ...A.T...... [ 90] O.apodus_194938 ..C...... C.---.. TAAAA.A.C...... CG...... [ 90]

C.agasepsoides_160191_Canoa CTTTGCCACA CCTACAC-AG GTCATCAGCA GTAATTAA-C ATTAG [ 135] C.curtissi_aporus_102610_La_Descubierta ...... -.. ...C...... -...... [ 135] C.badius_194964_Navassa_Island ...... -.. .C.C...... -...... [ 135] C.barbori_161120_Quick_Step ...... -.. ...T...... -...... [ 135] C.costatus_costatus_192054_Abricots ...... -.. .C.C...... -. ....A [ 135] C.darlingtoni_161687_Cacique_Enriquillo ...... -.. ..TT...... -...... [ 135] C.maculatus_266527_Cayman_Brac ...... -.. .C.C...... -...... [ 135] C.marcanoi_193286_Valle_de_Bao ...... -.. .C.C...... -. ....A [ 135] C.costatus_neiba_192428_Ca_Soleil ...... -.. ...C...... -...... [ 135] C.costatus_oriestes_104408_Jacmel ...... -.. .C.C...... -. ....A [ 135] C.stenurus_rugosus_103083_Nagua ...... -.. .C.C...... -...... [ 135] C.sepsoides_102369_Sabana_de_la_Mar ...... -.. ...C...... AT ....A [ 135] C.warreni_172914_Hispaniola ...... -...... -...... [ 135] C.warreni_194521_Puerto_Plata ...... -...... -...... [ 135] D.nigropuntatus_191015_Cuba T...A...... -.. ...C...... -A ..... [ 135] D.nigropunctatus_191320_Cuba T...A...... -.. ...C...... -A ..... [ 135] D.nigropunctatus_191542_Cuba T...A...... -.. ...CC...... -A ..... [ 135] D.pleii_191574_Puerto_Rico T..C...... C.. .C.C...... -A ..... [ 135] C.crusculus_101572_Content ...... G ...... -.. ...T...... -. ....A [ 135] C.crusculus_172442_Duncans ...... -.. ...T...... -. ....A [ 135] C.cyanochloris_194930_Costa_Rica ...... -.. ..AC...... -...... [ 135] C.enneagrammus_191044_Mexico_23 ...... -.. .CAC...... -...... [ 135] O.apodus_194938 ...... T ...C...-.. ..A...... C..-...... [ 135]

C.agasepsoides_160191_Canoa ACAATAAGCG AAA-GCTTGA CCTAGATATG ACTAACCA-A GGCCG [ 180] C.curtissi_aporus_102610_La_Descubierta G.C...... -...... C... .T..-T.TAG ..... [ 180] C.badius_194964_Navassa_Island G.C...... -...... TC...TT-G ..... [ 180] C.barbori_161120_Quick_Step ..C...... -...... T.....T-G ..... [ 180] C.costatus_costatus_192054_Abricots ..C..G...... -...... T...T.TAG ..... [ 180] C.darlingtoni_161687_Cacique_Enriquillo G.C...... -...... C.... .T....G.-G ..... [ 180] C.maculatus_266527_Cayman_Brac G.C....A.. ...-...... TC...TT-G ..... [ 180] C.marcanoi_193286_Valle_de_Bao ..C...... -...... C... .T...TTTAG ..... [ 180] C.costatus_neiba_192428_Ca_Soleil ..C...... -...... T...TTTAG ..... [ 180] C.costatus_oriestes_104408_Jacmel G.C...... -...... T....TTGG ..... [ 180] C.stenurus_rugosus_103083_Nagua G.C...... -...... TC...TC-G ..... [ 180] C.sepsoides_102369_Sabana_de_la_Mar C.C...... -...... A....C.... .T...-.TAG ..... [ 180] C.warreni_172914_Hispaniola G.C...... -...C...... C.... .T...... -G ..... [ 180] C.warreni_194521_Puerto_Plata G.C...... -...C...... C.... .T.....C-G ..... [ 180] D.nigropuntatus_191015_Cuba G.C.....T. ...-A..C...... T..A. .T...--CAG A.... [ 180] D.nigropunctatus_191320_Cuba G.C.....T. ...-A..C...... T..A. .T...--CAG A.... [ 180] D.nigropunctatus_191542_Cuba G.C.....T. ...-...C...... T..A. .T...--CAG A.... [ 180] D.pleii_191574_Puerto_Rico G...... T. ...-A...... C..C..C. GT...--TAG AA... [ 180] C.crusculus_101572_Content G.C...... -...... T....TT-G ..... [ 180] C.crusculus_172442_Duncans G.C...... -...... GT...TTT-G ..... [ 180] C.cyanochloris_194930_Costa_Rica G.C...... C..-...... T.T--ACAG ..... [ 180] C.enneagrammus_191044_Mexico_23 G.C...... A...... C.... .T.T--A.A...... [ 180] O.apodus_194938 G.C...... C..-...... C...... TC-ACAG ..... [ 180]

C.agasepsoides_160191_Canoa GTTAATTTCG TGCCAGCAA- CCGCGGTTAT ACGAAAAGCC CAAAA [ 225] C.curtissi_aporus_102610_La_Descubierta ...... - ...... [ 225] C.badius_194964_Navassa_Island ...... - ...... G.. [ 225] C.barbori_161120_Quick_Step ...... - ...... [ 225] C.costatus_costatus_192054_Abricots ..C...... - ...... [ 225] C.darlingtoni_161687_Cacique_Enriquillo ...... - ...... [ 225] C.maculatus_266527_Cayman_Brac ...... - ...... G.. [ 225] C.marcanoi_193286_Valle_de_Bao ...... - ...... [ 225] C.costatus_neiba_192428_Ca_Soleil ...... - ...... [ 225] C.costatus_oriestes_104408_Jacmel ...... - ...... [ 225] C.stenurus_rugosus_103083_Nagua ...... - ...... G.. [ 225] C.sepsoides_102369_Sabana_de_la_Mar ..C...... - ...... [ 225] C.warreni_172914_Hispaniola ...... - ...... [ 225] C.warreni_194521_Puerto_Plata ...... - ...... [ 225] D.nigropuntatus_191015_Cuba ..A...... - ...... C ..A...G...... [ 225] D.nigropunctatus_191320_Cuba ..A...... - ...... C ..A...G...... [ 225] D.nigropunctatus_191542_Cuba ..A...... - ...... C ..A...G...... [ 225] D.pleii_191574_Puerto_Rico ..A...... - ...... T...... [ 225] C.crusculus_101572_Content ...... - ...... [ 225] C.crusculus_172442_Duncans ...... - ...... G... [ 225] C.cyanochloris_194930_Costa_Rica ..A...... - ...... [ 225] C.enneagrammus_191044_Mexico_23 ..A...... A T...... T.... [ 225] O.apodus_194938 ..A...... - ...... G... T.... [ 225]

C.agasepsoides_160191_Canoa -TAGTACAAC AACGGCGTAA AGAG-TGACT AGAA---ATG ACTCC [ 270] C.curtissi_aporus_102610_La_Descubierta -...C...... -...... -----.T ..C.. [ 270] C.badius_194964_Navassa_Island -...C....T ...... -...... ---.CA C.... [ 270] C.barbori_161120_Quick_Step -...C...... G...... -...... ---.CA C.... [ 270] C.costatus_costatus_192054_Abricots -...C...... -...... ---.CA C.... [ 270] C.darlingtoni_161687_Cacique_Enriquillo -...CG...G ...... -...... ---G...... [ 270] C.maculatus_266527_Cayman_Brac -...CG...... -...... ---GCA C.... [ 270] C.marcanoi_193286_Valle_de_Bao -...C...... -...... -----.T .TC.. [ 270] C.costatus_neiba_192428_Ca_Soleil -...C...... -...... -----.T ..C.. [ 270] C.costatus_oriestes_104408_Jacmel -...C...... -...... ---.CA C.... [ 270] C.stenurus_rugosus_103083_Nagua -...C...... -...... ---GCA C.... [ 270] C.sepsoides_102369_Sabana_de_la_Mar -...C...-...... -...... G---..A C.A.. [ 270] C.warreni_172914_Hispaniola -....G...T ...... G.-...... ---.CA ..C.. [ 270] C.warreni_194521_Puerto_Plata -....G...T ...... G.-...... ---.CA ..C.. [ 270] D.nigropuntatus_191015_Cuba -..A..-.C...... C.-....C ...CA----T CAC.. [ 270] D.nigropunctatus_191320_Cuba -..A..-.C...... C.-....C ...CA----T CAC.. [ 270] D.nigropunctatus_191542_Cuba -..A..-.C...... C.-....C ...CA----T CGC.. [ 270] D.pleii_191574_Puerto_Rico -..AC.-.C...... C.G...... CCCAC.T T.C.. [ 270] C.crusculus_101572_Content -...C...... -...... ---.CA C.... [ 270] C.crusculus_172442_Duncans -...C...... -...... T--..T C.... [ 270] C.cyanochloris_194930_Costa_Rica -..AC.T.-- ...... A.. ..C.-...... TC-CAT T.AT. [ 270] C.enneagrammus_191044_Mexico_23 A..T..T.-- C...... A.. ..C.-...... CT-T.T .TA.. [ 270] O.apodus_194938 -..A.G.C-- C...... C.-...... CT-CCC C.-.T [ 270]

C.agasepsoides_160191_Canoa GCGGCCCTTA AAAACAAAAC CCAATTTTAG TAGTAAAATA CCAAA [ 315] C.curtissi_aporus_102610_La_Descubierta ..AA....A. ...CA..-.. A...C...... [ 315] C.badius_194964_Navassa_Island A.A.....A. ...CA..-.. ...GC..... C...... G ..... [ 315] C.barbori_161120_Quick_Step T.AA....C. ...CA..-...... C...... GA.G...... [ 315] C.costatus_costatus_192054_Abricots A.A.....A. ...CA..-...... C...... [ 315] C.darlingtoni_161687_Cacique_Enriquillo ..A.....A. ...CA..-...... C...... GA...... [ 315] C.maculatus_266527_Cayman_Brac A.AA....A. ...CA..-.. ...GA...... [ 315] C.marcanoi_193286_Valle_de_Bao A.A.....A. ...CA..-.. A...C...... [ 315] C.costatus_neiba_192428_Ca_Soleil A.A.....A. ...CA..-.. A...C...... G...... [ 315] C.costatus_oriestes_104408_Jacmel ..A.....A. ...CA..G...... C...... [ 315] C.stenurus_rugosus_103083_Nagua A.A.....A. ...CA..-...... C...... G ..... [ 315] C.sepsoides_102369_Sabana_de_la_Mar A.A.....A. ...CA..-...... C...... [ 315] C.warreni_172914_Hispaniola C.A.....A. ...CA..-.T ....C...... GA..G...... [ 315] C.warreni_194521_Puerto_Plata C.A.....A. ...CA..-.T ....C...... GA..G...... [ 315] D.nigropuntatus_191015_Cuba A.CC.A..A. .GG..C.C.A ....C.-A.. C...... G ..... [ 315] D.nigropunctatus_191320_Cuba A.CC.A..A. .GG.TC.C.A ....C.-A.. C...... G ..... [ 315] D.nigropunctatus_191542_Cuba A.CC....A. .GG.TC.C.A ....C.-A.. C...... G ..... [ 315] D.pleii_191574_Puerto_Rico C.CC.A..A. .GG..C.C.A .A.GC..A.. C.....T..G .T... [ 315] C.crusculus_101572_Content A.A.....A. ...CA..-...... C...... G...... [ 315] C.crusculus_172442_Duncans ..A.....A. ...CA..-...... C...... G...... [ 315] C.cyanochloris_194930_Costa_Rica TGATG.AAA. .G.TA..--. TA.GAC.C.. C...... CG ..T.. [ 315] C.enneagrammus_191044_Mexico_23 A.CAA...A. ...TTG.--A AGCCCC.C.. C...... G ..C.. [ 315] O.apodus_194938 ..CCA.T.A. ...-G..--. TA.GA...... T..G...C...... [ 315]

C.agasepsoides_160191_Canoa A-ACCAAAGA -ATACCCAAC TTTTAAACCC AG-AGGACCT TTAA- [ 360] C.curtissi_aporus_102610_La_Descubierta .-G..T.... -.....AT-A ....-.G... T.C...... - [ 360] C.badius_194964_Navassa_Island .-...T.... -...... T...... -..... T.A...... - [ 360] C.barbori_161120_Quick_Step .-...T.... -....T.T...... T..... T.C...... - [ 360] C.costatus_costatus_192054_Abricots .-G..T.... -.....AC-A ....T..... T.C...... - [ 360] C.darlingtoni_161687_Cacique_Enriquillo .-...T.... -.....TT.T ....-..... T.C...... C ....- [ 360] C.maculatus_266527_Cayman_Brac .-...T.... -...... T...... T.G... T.C...... - [ 360] C.marcanoi_193286_Valle_de_Bao .-G..T.... -.....AC-A ....T.G... T.C...... - [ 360] C.costatus_neiba_192428_Ca_Soleil .-G..T.... -.....AT-A ....T..... T.C...G... ..G.- [ 360] C.costatus_oriestes_104408_Jacmel .-G..T.... -.....AT-A ....T.C... T.C...... G [ 360] C.stenurus_rugosus_103083_Nagua .-...C.... -...... T.A ....T.G... TAT...... - [ 360] C.sepsoides_102369_Sabana_de_la_Mar .-GT.T.... -.....A--A ....T.G... T.C...G...... - [ 360] C.warreni_172914_Hispaniola .-...T.... -.....TT.T ....T.G... T.C...... C ....- [ 360] C.warreni_194521_Puerto_Plata .-...T.... -.....TT.T ....T.G... T.C...... C ....- [ 360] D.nigropuntatus_191015_Cuba GC.-AT..A. -.C.T.ATCA CC..T....A GACG.....A CC..G [ 360] D.nigropunctatus_191320_Cuba GC.-AT..A. -.C.T.ATCA CC..T....A -ACG.....A CC..- [ 360] D.nigropunctatus_191542_Cuba GC.GAT..A. G.C.T.ATCA CC..T....A GACG.....A CC..G [ 360] D.pleii_191574_Puerto_Rico .T.--TG.A. -.....ACCA CC..T.G... -AC...-.TA .C..- [ 360] C.crusculus_101572_Content .-...T.... -...... T-. C...T..... T.C...... - [ 360] C.crusculus_172442_Duncans .-...T.... -...... TG. C...T...T. T.C...... - [ 360] C.cyanochloris_194930_Costa_Rica G-T..C.... ACAC.AACC. .C...-G.TA ..CT.A..A- ....- [ 360] C.enneagrammus_191044_Mexico_23 .-CTT..... ACCC.AGCCT .....TG.TT C.CT...TAA ..G.- [ 360] O.apodus_194938 .-T...... A.CC.A---. CC....GAAA GCAC...... - [ 360]

C.agasepsoides_160191_Canoa CTCACAAAA- -ACTAAGAAA CAA--ACTAG GATTAGATAC CCTAT [ 405] C.curtissi_aporus_102610_La_Descubierta ...... - -...... --...... [ 405] C.badius_194964_Navassa_Island ...... - -...... --...... [ 405] C.barbori_161120_Quick_Step G...... - -...... --...... [ 405] C.costatus_costatus_192054_Abricots ...... - -...... GC.--...... [ 405] C.darlingtoni_161687_Cacique_Enriquillo ...... - -...... --...... [ 405] C.maculatus_266527_Cayman_Brac ...... - -...... --...... [ 405] C.marcanoi_193286_Valle_de_Bao ...... - -...... --...... [ 405] C.costatus_neiba_192428_Ca_Soleil ...... - -...... --...... [ 405] C.costatus_oriestes_104408_Jacmel ...... - -...... --...... -.C [ 405] C.stenurus_rugosus_103083_Nagua ...... - -...... --...... [ 405] C.sepsoides_102369_Sabana_de_la_Mar ...... - -...... --...... [ 405] C.warreni_172914_Hispaniola ...... - -...... --...... [ 405] C.warreni_194521_Puerto_Plata ...... - -...... --...... [ 405] D.nigropuntatus_191015_Cuba .....G...G -...... GAG...... C [ 405] D.nigropunctatus_191320_Cuba .....G...- -...... --...... C [ 405] D.nigropunctatus_191542_Cuba .....G...A CG...... -AG...... C [ 405] D.pleii_191574_Puerto_Rico ...... - -...... -AG...... C [ 405] C.crusculus_101572_Content ...... - -...... T ...--...... [ 405] C.crusculus_172442_Duncans ...... - -...... --...... [ 405] C.cyanochloris_194930_Costa_Rica ...... G- -....G...... --...... C [ 405] C.enneagrammus_191044_Mexico_23 ...... - -...... --...... C [ 405] O.apodus_194938 .....T...- -...... --...... C [ 405]

C.agasepsoides_160191_Canoa TATGCTTAGT CGTAA-CCTG ATAC-AGTAC ATACTAACAA TCCGC [ 450] C.curtissi_aporus_102610_La_Descubierta ...... T....-...... -....T ....C...... [ 450] C.badius_194964_Navassa_Island ...... -...... GA-...... C...... [ 450] C.barbori_161120_Quick_Step ...... C...... -...... -...... C...... G ..... [ 450] C.costatus_costatus_192054_Abricots ...... C...... -...... T.-....T ....C...... [ 450] C.darlingtoni_161687_Cacique_Enriquillo ...... C...... A...... -T...T ....C...... [ 450] C.maculatus_266527_Cayman_Brac ...... -...... A-...... C.G...... [ 450] C.marcanoi_193286_Valle_de_Bao ...... -...... -....A ....C...... [ 450] C.costatus_neiba_192428_Ca_Soleil ...... C...... -...... -....T ....C...... [ 450] C.costatus_oriestes_104408_Jacmel ...... C... G....-...... T-...... C..C.G..G ..... [ 450] C.stenurus_rugosus_103083_Nagua ...... C .....-...... A-....T ....C.G...... [ 450] C.sepsoides_102369_Sabana_de_la_Mar ...... C...... -.T...... -.A..T G...C.C...... [ 450] C.warreni_172914_Hispaniola ...... C...... -...... -TA.GT ....C..T...... [ 450] C.warreni_194521_Puerto_Plata ...... C...... -...... -TA..T ....C..T...... [ 450] D.nigropuntatus_191015_Cuba ...... C...... C.ACA GAT.--AA.. CA..AC.TG...... [ 450] D.nigropunctatus_191320_Cuba ...... C... T....C.ACA GAT.--AA.. CA..AC.TG...... [ 450] D.nigropunctatus_191542_Cuba ...... C...... C.ACA GAT.T.AA.. CA..AC.TG...... [ 450] D.pleii_191574_Puerto_Rico ...... C... .A.G.C.T.. ..T.------A..AC.TG- - .... [ 450] C.crusculus_101572_Content ...... C...... -...... -...T. .G..C...G...... [ 450] C.crusculus_172442_Duncans ...... C...... -...... -....T ....C...... [ 450] C.cyanochloris_194930_Costa_Rica .....C..A. TA...C.T.A GAC-----C. CC------G ..... [ 450] C.enneagrammus_191044_Mexico_23 ...... T...C.T.A GAT-----TA C------...... [ 450] O.apodus_194938 ...... C...T..AA GATATTTAC. CC.....AC...... [ 450]

C.agasepsoides_160191_Canoa CAG-AGAACT ACAAGTGAAA AACTTAAAAC TCAAAGGATT TGGCG [ 495] C.curtissi_aporus_102610_La_Descubierta ...-..G...... [ 495] C.badius_194964_Navassa_Island ...-...... [ 495] C.barbori_161120_Quick_Step ...-...... [ 495] C.costatus_costatus_192054_Abricots ...-...... [ 495] C.darlingtoni_161687_Cacique_Enriquillo ...-..G...... [ 495] C.maculatus_266527_Cayman_Brac ...-...... GG...... [ 495] C.marcanoi_193286_Valle_de_Bao ...-..G...... [ 495] C.costatus_neiba_192428_Ca_Soleil ...-..G...... [ 495] C.costatus_oriestes_104408_Jacmel ...-...... -...... [ 495] C.stenurus_rugosus_103083_Nagua ...-...... [ 495] C.sepsoides_102369_Sabana_de_la_Mar ...-...... [ 495] C.warreni_172914_Hispaniola ...-..G.T...... [ 495] C.warreni_194521_Puerto_Plata ...-..G...... [ 495] D.nigropuntatus_191015_Cuba ...G...... G...... C...... C...... [ 495] D.nigropunctatus_191320_Cuba ...-...... G...... C...... C...... [ 495] D.nigropunctatus_191542_Cuba ...-...... G...... T ...C...... C...... [ 495] D.pleii_191574_Puerto_Rico ...-...... G...... C...... [ 495] C.crusculus_101572_Content ...-...... [ 495] C.crusculus_172442_Duncans ...-...... [ 495] C.cyanochloris_194930_Costa_Rica ...-...... C..C.... .G...... [ 495] C.enneagrammus_191044_Mexico_23 ...-...... T...... [ 495] O.apodus_194938 ...-...... G...... [ 495]

C.agasepsoides_160191_Canoa GTGCTCCAAC C-GACCTAGA G-GAGCCTGT CCT--ATAAT C- -GA [ 540] C.curtissi_aporus_102610_La_Descubierta ...... A-.-...... -...... --...... - -.. [ 540] C.badius_194964_Navassa_Island ...... -A...... -...... --...... - -.. [ 540] C.barbori_161120_Quick_Step ...... -A...... -...... --...... - -.. [ 540] C.costatus_costatus_192054_Abricots ...... A-.-...... -...... --...... - -.. [ 540] C.darlingtoni_161687_Cacique_Enriquillo ...... -AGAT.... .-...... --TC... .- -.. [ 540] C.maculatus_266527_Cayman_Brac ...... -A...... -...... --...... - -.. [ 540] C.marcanoi_193286_Valle_de_Bao ...... A-A-.T.... .-...... --..... TCG.. [ 540] C.costatus_neiba_192428_Ca_Soleil ...... A-.-...... -...... --...... - -.. [ 540] C.costatus_oriestes_104408_Jacmel ...... A-..T...... -...... --...... - -.. [ 540] C.stenurus_rugosus_103083_Nagua ...... -A...... A...... --...... - -.. [ 540] C.sepsoides_102369_Sabana_de_la_Mar ...... -AG...... -...... --.C... .- -.. [ 540] C.warreni_172914_Hispaniola ...... -A...... -...... --.C... .- -.. [ 540] C.warreni_194521_Puerto_Plata ...... -A...... -...... T-.C... .- -.. [ 540] D.nigropuntatus_191015_Cuba ...... TC.. .CA..T.... .-...... TAT..T. .- -.. [ 540] D.nigropunctatus_191320_Cuba ...... TT.. .CA...... -...... T.A--.... .- -.. [ 540] D.nigropunctatus_191542_Cuba ...... TT.. .CA...... -...... T.A--.... .- -.. [ 540] D.pleii_191574_Puerto_Rico ...... TT.. .CA...... -...... - .T.A--.... .- -.. [ 540] C.crusculus_101572_Content ...... -..T...... -...... --...... - -.. [ 540] C.crusculus_172442_Duncans ...... -..T...... -...... --...... - -.. [ 540] C.cyanochloris_194930_Costa_Rica ...... C. .-A...... -...... T..--...... - -.. [ 540] C.enneagrammus_191044_Mexico_23 ...... CT .-A...... -....T... T..--G.... .- -.. [ 540] O.apodus_194938 ...... CT .-A...... -...... --...... - -.. [ 540]

C.agasepsoides_160191_Canoa TACCCCACGA TCAACCTCAC CATC-TTTG- CC-CTCCAGC ATATA [ 585] C.curtissi_aporus_102610_La_Descubierta ...T...... TG...... T....- ..-.CT.... T.... [ 585] C.badius_194964_Navassa_Island ...T...... C....- ..-AC..... T.... [ 585] C.barbori_161120_Quick_Step ...... T...... T....- ..-AC..... T.... [ 585] C.costatus_costatus_192054_Abricots ...... T...... T....- ..-.CT.... T.... [ 585] C.darlingtoni_161687_Cacique_Enriquillo ..TT...... C....- ..-AC..... T.... [ 585] C.maculatus_266527_Cayman_Brac ...T...... C....- ..-AC..... T.... [ 585] C.marcanoi_193286_Valle_de_Bao ...T...... TG...... T....- ..-.CT.... T.... [ 585] C.costatus_neiba_192428_Ca_Soleil ...T...... T...... T....- ..-.CT.... T.... [ 585] C.costatus_oriestes_104408_Jacmel ...T...... T...... T....- ..-AC..... T.... [ 585] C.stenurus_rugosus_103083_Nagua ...T...... C....G ..-AC..... T.... [ 585] C.sepsoides_102369_Sabana_de_la_Mar ...... T....- ..-.C-.... T.... [ 585] C.warreni_172914_Hispaniola ...T...... C....- ..-AC..... T.... [ 585] C.warreni_194521_Puerto_Plata ...T...... C....- ..-AC..... T.... [ 585] D.nigropuntatus_191015_Cuba ...... CTC....- .A-.A...A. T.... [ 585] D.nigropunctatus_191320_Cuba ...... CTC....- .A-.A...A. T.... [ 585] D.nigropunctatus_191542_Cuba ...... CTC....- .A-.A...A. T.... [ 585] D.pleii_191574_Puerto_Rico ...... CTA....- .A-.AT.... T.... [ 585] C.crusculus_101572_Content ...T...... T...... T....- ..-AC..... T.... [ 585] C.crusculus_172442_Duncans ...T...... T...... T....- ..-AC..... T.... [ 585] C.cyanochloris_194930_Costa_Rica ...... G ..--...... T..A.T T.A.CA.... C.... [ 585] C.enneagrammus_191044_Mexico_23 ...T...... C.C..A.C .--.CT.... T.... [ 585] O.apodus_194938 ...... C...... CC.CC.G.C .TAAC..... C.... [ 585]

C.agasepsoides_160191_Canoa TACCGCCGTC GTCAGCCTAT CTTGTGAAAG ACCTTAAATA GGCAA [ 630] C.curtissi_aporus_102610_La_Descubierta ...... T... .C...... A...... A.... [ 630] C.badius_194964_Navassa_Island ...... T... .C...... T. CT.CC..... A.... [ 630] C.barbori_161120_Quick_Step ...... C...... T.C...... A.... [ 630] C.costatus_costatus_192054_Abricots ...... T... .C.A...... A...... A.... [ 630] C.darlingtoni_161687_Cacique_Enriquillo ...... T... .C...... C...... A.... [ 630] C.maculatus_266527_Cayman_Brac ...... T... .C...... T.C...... A.... [ 630] C.marcanoi_193286_Valle_de_Bao ...... T... .C...... C...... A.... [ 630] C.costatus_neiba_192428_Ca_Soleil ...... T... .C...... C...... A.... [ 630] C.costatus_oriestes_104408_Jacmel ...... C....T... .C...... T.C...... A.... [ 630] C.stenurus_rugosus_103083_Nagua ...... T... .C...... T.C...... A.... [ 630] C.sepsoides_102369_Sabana_de_la_Mar ...... T... .C...... C...... A...T [ 630] C.warreni_172914_Hispaniola ...... C...... G. .TTC...... T [ 630] C.warreni_194521_Puerto_Plata ...... C...... G. .TTC...... T [ 630] D.nigropuntatus_191015_Cuba ...... C...... A...... A.AA...C...... [ 630] D.nigropunctatus_191320_Cuba ...... C...... A...... A.AA...C...... [ 630] D.nigropunctatus_191542_Cuba ...... C...... A...... A.AA...... [ 630] D.pleii_191574_Puerto_Rico ...... C....T...... ACA.G.. .A.AA..... A.... [ 630] C.crusculus_101572_Content ...... T... .C...... C...... A.... [ 630] C.crusculus_172442_Duncans ...... C....T... .C...... C...... A.... [ 630] C.cyanochloris_194930_Costa_Rica ...... A.. .C....T...... CA...A C..AA..... A.... [ 630] C.enneagrammus_191044_Mexico_23 ...... T... .C..CA...... TAA..... A.... [ 630] O.apodus_194938 ...... C.....GG. ..AAA..... A...T [ 630] C.agasepsoides_160191_Canoa AATAGT-CCA CCACTAAAAC GTCAGGTCAA GGCGTAGCTA ATAAG [ 675]

C.curtissi_aporus_102610_La_Descubierta G....C-.A. ..G...... [ 675] C.badius_194964_Navassa_Island ....AC-.A. ..G....T...... [ 675] C.barbori_161120_Quick_Step ...... -.A...... [ 675] C.costatus_costatus_192054_Abricots G....C-.A. ..G...... [ 675] C.darlingtoni_161687_Cacique_Enriquillo .....C-...... [ 675] C.maculatus_266527_Cayman_Brac ..G...-.A...... T...... [ 675] C.marcanoi_193286_Valle_de_Bao G....C-.A. ..G...... [ 675] C.costatus_neiba_192428_Ca_Soleil G....C-.A. ..G...... [ 675] C.costatus_oriestes_104408_Jacmel ...... -.A...... [ 675] C.stenurus_rugosus_103083_Nagua ...... -.A...... [ 675] C.sepsoides_102369_Sabana_de_la_Mar ...... -..C ...... [ 675] C.warreni_172914_Hispaniola .....C-.T...... [ 675] C.warreni_194521_Puerto_Plata .....C-.T...... [ 675] D.nigropuntatus_191015_Cuba ..C...A...... T.T ...GA [ 675] D.nigropunctatus_191320_Cuba ..C...A...... T.T ...GA [ 675] D.nigropunctatus_191542_Cuba ..C...A...... T.T ...GA [ 675] D.pleii_191574_Puerto_Rico ...... A...... T ....A [ 675] C.crusculus_101572_Content ...... -.A...... [ 675] C.crusculus_172442_Duncans ...... -.A...... [ 675] C.cyanochloris_194930_Costa_Rica ...... C-T...... G.. [ 675] C.enneagrammus_191044_Mexico_23 ...... T-.C ...... T ...G. [ 675] O.apodus_194938 ..C...TG.T ...... GG. [ 675] C.agasepsoides_160191_Canoa ATGGCCAGAG ATGGGCTACA CTCTCCTCCA ACAAGAGAAA ACGAA [ 720] C.curtissi_aporus_102610_La_Descubierta ...... T..A. C-...... [ 720]

C.badius_194964_Navassa_Island ...... TT..A. C-...... [ 720] C.barbori_161120_Quick_Step ...... C.T..A. C-...... [ 720] C.costatus_costatus_192054_Abricots ...... T..A. C-...... G ..... [ 720] C.darlingtoni_161687_Cacique_Enriquillo ...... T..A. C-...... G ..... [ 720] C.maculatus_266527_Cayman_Brac ...... TC.A. C-...... [ 720] C.marcanoi_193286_Valle_de_Bao ...... T..A. C-...... G ..... [ 720] C.costatus_neiba_192428_Ca_Soleil ...... T..A. C-...... G ..... [ 720] C.costatus_oriestes_104408_Jacmel ...... TC.A. C-...... [ 720] C.stenurus_rugosus_103083_Nagua ...... T..A. CT...... [ 720] C.sepsoides_102369_Sabana_de_la_Mar ....T...... T..A. C-....A...... [ 720] C.warreni_172914_Hispaniola ...... TC.A. C-...... [ 720] C.warreni_194521_Puerto_Plata ...... TC.A. C-...... [ 720] D.nigropuntatus_191015_Cuba GC..-..A...... TA... C--...A...... [ 720] D.nigropunctatus_191320_Cuba GC..-..A...... TA... C--...A...... [ 720] D.nigropunctatus_191542_Cuba GC..-..A...... TA... C--...A...... [ 720] D.pleii_191574_Puerto_Rico G...-..A...... T..TA..C C--...A...... [ 720] C.crusculus_101572_Content ...... TCAA. C-...... [ 720] C.crusculus_172442_Duncans ...... T.AA. C-...... [ 720] C.cyanochloris_194930_Costa_Rica ....-...C...... TAAA. C--...... [ 720] C.enneagrammus_191044_Mexico_23 G...-...T...... TA.A. T--....G...... [ 720] O.apodus_194938 GC..-...C...... TA.T. .--...AG.. ..A.. [ 720]

C.agasepsoides_160191_Canoa AGACACAGTG -AAACCAATA CCA-GAAGGC GGATTTAGAA GTAAA [ 765] C.curtissi_aporus_102610_La_Descubierta .....A.A.. -....G...G ...-...... [ 765]

C.badius_194964_Navassa_Island ...... A.. -...... G.G ...-...... [ 765] C.barbori_161120_Quick_Step .....T.... -.....T..G ...-...... [ 765] C.costatus_costatus_192054_Abricots .....A.A.. -....G...G ...-...... [ 765] C.darlingtoni_161687_Cacique_Enriquillo ...... A.. -...... G.G ...-...... [ 765] C.maculatus_266527_Cayman_Brac .....T.... -...... G ...-...... [ 765] C.marcanoi_193286_Valle_de_Bao .....A.A.. -....A.G.G ...-...... [ 765] C.costatus_neiba_192428_Ca_Soleil .....A.A.. -....G...G ...-...... [ 765] C.costatus_oriestes_104408_Jacmel ...... -...... G.G ...-...... [ 765] C.stenurus_rugosus_103083_Nagua ...... -.....TG.G ...-...... [ 765] C.sepsoides_102369_Sabana_de_la_Mar .AG...T... -...... G.G ...-...... [ 765] C.warreni_172914_Hispaniola ...... A.. -...... G.G ...-.....T ...... T...... [ 765] C.warreni_194521_Puerto_Plata ...... A.. -...... G.G ...-.....T ...... T...... [ 765] D.nigropuntatus_191015_Cuba CA...A.A.. -....A...G T..CA...... [ 765] D.nigropunctatus_191320_Cuba CA...A.A.. -....A...G T..CA...... [ 765] D.nigropunctatus_191542_Cuba CA...A.A.. -....A.G.G T..CA...... [ 765] D.pleii_191574_Puerto_Rico T.G..A.A.. -..T.A...G ...-A...... [ 765] C.crusculus_101572_Content ...... -.....CG.G ...-...... [ 765] C.crusculus_172442_Duncans ...... -.....C..G ...-...... [ 765] C.cyanochloris_194930_Costa_Rica ...T.T.... C..C..T... T..-A...... [ 765] C.enneagrammus_191044_Mexico_23 .AG...... -.....TG.G .T.-A...... [ 765] O.apodus_194938 T.G.CT.A.. -...TTT.C. ...-A...... C...... [ 765]

C.agasepsoides_160191_Canoa ATTAACCAGA ATATTAATTT TAAA-CAAGC CCTGGAGCGC GCACA [ 810] C.curtissi_aporus_102610_La_Descubierta .....T..T...... -...... [ 810]

C.badius_194964_Navassa_Island ...... -...... [ 810] C.barbori_161120_Quick_Step ...... -.T...... [ 810] C.costatus_costatus_192054_Abricots .....T...... -...... [ 810] C.darlingtoni_161687_Cacique_Enriquillo ...... C...... -...... [ 810] C.maculatus_266527_Cayman_Brac ...... -...... [ 810] C.marcanoi_193286_Valle_de_Bao .....T...... -A...... [ 810] C.costatus_neiba_192428_Ca_Soleil .....T..T...... -A...... [ 810] C.costatus_oriestes_104408_Jacmel ...... G...... --...... [ 810] C.stenurus_rugosus_103083_Nagua ...... -...... [ 810] C.sepsoides_102369_Sabana_de_la_Mar ...... -...... AT ..... [ 810] C.warreni_172914_Hispaniola ...... C...... -...... [ 810] C.warreni_194521_Puerto_Plata ...... C...... -...... [ 810] D.nigropuntatus_191015_Cuba .C..GA.... .C.C...C.. -...-.T... .A.A....A...... [ 810] D.nigropunctatus_191320_Cuba .C..GA.... .C.C...C.. -...-.T... .A.A....A...... [ 810] D.nigropunctatus_191542_Cuba ....GA.... .C.C...C.. -...-.T... .A.A....A...... [ 810] D.pleii_191574_Puerto_Rico .C..GA.... .C.C...C.. -...-.C... .A.A...... [ 810] C.crusculus_101572_Content ...... G...... A...-A-...... [ 810] C.crusculus_172442_Duncans ...... G...... -A-...... [ 810] C.cyanochloris_194930_Costa_Rica ....G...... C.C...C...... -AC...... [ 810] C.enneagrammus_191044_Mexico_23 ..G...... C.... A...-AC...... [ 810] O.apodus_194938 .CA.G.A... G.GC.TT... A...TAT...... [ 810]

C.agasepsoides_160191_Canoa CACCGCCCGT CACCCTCCTC AAAAACAATA -CAAAT---A ACCAT [ 855] C.curtissi_aporus_102610_La_Descubierta ...... -..C. -....C---...... [ 855]

C.badius_194964_Navassa_Island ...... C. -....C---...... [ 855] C.barbori_161120_Quick_Step ...... C. -....C---...... [ 855] C.costatus_costatus_192054_Abricots ...... -..C. -T..GC---...... [ 855] C.darlingtoni_161687_Cacique_Enriquillo ...... C. -.G..C---...... [ 855] C.maculatus_266527_Cayman_Brac ...... GC. -T...C---...... [ 855] C.marcanoi_193286_Valle_de_Bao ...... -..C. -...GC---...... [ 855] C.costatus_neiba_192428_Ca_Soleil ...... -..C. -....C---...... [ 855] C.costatus_oriestes_104408_Jacmel ...... C. A....C---...... [ 855] C.stenurus_rugosus_103083_Nagua ...... GC. -....C---...... [ 855] C.sepsoides_102369_Sabana_de_la_Mar ...... GC....C. -....C---. .T... [ 855] C.warreni_172914_Hispaniola ...... G.C. -TG..C---. .T... [ 855] C.warreni_194521_Puerto_Plata ...... C. -TG.-C---. .T... [ 855] D.nigropuntatus_191015_Cuba ...... CT.A---. TT...CCTA. .T... [ 855] D.nigropunctatus_191320_Cuba ...... CT.A---. TT...CCTA. .T... [ 855] D.nigropunctatus_191542_Cuba ...... CT.A---. TT...CCTA. .- ... [ 855] D.pleii_191574_Puerto_Rico .-...... GCC.ATCA. C....CC--...... [ 855] C.crusculus_101572_Content ...... C. -....C---...... [ 855] C.crusculus_172442_Duncans ...... C. -....C---...... [ 855] C.cyanochloris_194930_Costa_Rica ...... C.CACCC. CAG.CCTT-T .AT.. [ 855] C.enneagrammus_191044_Mexico_23 ...... C.C---.. CA..CCAGAT .AT.. [ 855] O.apodus_194938 ...... C..--CC. C...C.CTA. .A.-. [ 855]

C.agasepsoides_160191_Canoa ATTCAAATAA ATTTATCAAG ATGAGGCAAG TCGTAACATG GTAAG [ 900] C.curtissi_aporus_102610_La_Descubierta ...T..C... .AAC.C...... [ 900]

C.badius_194964_Navassa_Island ...AGCT... .AAC...... [ 900] C.barbori_161120_Quick_Step ...TT.CC.. .AAC...... [ 900] C.costatus_costatus_192054_Abricots ...T..C..T .AAC...... [ 900] C.darlingtoni_161687_Cacique_Enriquillo ...... CC.G .AA..C...... [ 900] C.maculatus_266527_Cayman_Brac ...A.CT... .AAC...... [ 900] C.marcanoi_193286_Valle_de_Bao ...T..C... .AAC.C...... [ 900] C.costatus_neiba_192428_Ca_Soleil ...T..C... .AAC.A...... [ 900] C.costatus_oriestes_104408_Jacmel ...T..CC.. .AAC...... [ 900] C.stenurus_rugosus_103083_Nagua ...AGCT... .AAC...... [ 900] C.sepsoides_102369_Sabana_de_la_Mar ...T..C... .AAC.C...... T...... [ 900] C.warreni_172914_Hispaniola ...T..C... G.A..CT...... [ 900] C.warreni_194521_Puerto_Plata ...T..C... ..A..CT...... [ 900] D.nigropuntatus_191015_Cuba ....C.C... CAAA...... T...... [ 900] D.nigropunctatus_191320_Cuba ....C.C... CAAA...... T...... [ 900] D.nigropunctatus_191542_Cuba ....C.C... CAAA.C...... T...... [ 900] D.pleii_191574_Puerto_Rico ...TCGTC.. .C.A.CT...... [ 900] C.crusculus_101572_Content ...T..CC.. .AAC...... [ 900] C.crusculus_172442_Duncans ...T..CC.. .AAC.C...... [ 900] C.cyanochloris_194930_Costa_Rica ..A.C..CCT .C.A.A.C.. .G...... [ 900] C.enneagrammus_191044_Mexico_23 ...TCCTAT. CA.C.A...... [ 900] O.apodus_194938 ..AA..TC.C .AA.CA.T...... [ 900]

C.agasepsoides_160191_Canoa CGCACTGGAA AGTGTGCTTG GAAT-CAAAA AGTAGCTTAC CCTTA [ 945] C.curtissi_aporus_102610_La_Descubierta ..T...... AC...... A..T...... A ..C.. [ 945]

C.badius_194964_Navassa_Island ...... C...... -..T...... A ..C.. [ 945] C.barbori_161120_Quick_Step ..T...... C...... A..T...... A ..C.. [ 945] C.costatus_costatus_192054_Abricots ..T...... AC...... A..T...... G ..C.. [ 945] C.darlingtoni_161687_Cacique_Enriquillo ...... C...... A...... G ..C.. [ 945] C.maculatus_266527_Cayman_Brac ...... C...... -..T...... A ..C.. [ 945] C.marcanoi_193286_Valle_de_Bao ..T...... AC...... A..T...... A T.C.. [ 945] C.costatus_neiba_192428_Ca_Soleil ...... AC...... A..T...... A ..C.. [ 945] C.costatus_oriestes_104408_Jacmel ...... C...... CA..T...... A T.C.. [ 945] C.stenurus_rugosus_103083_Nagua ...... C...... -..T...... A ..C.. [ 945] C.sepsoides_102369_Sabana_de_la_Mar ..T...... C...... -...... G ..C-. [ 945] C.warreni_172914_Hispaniola ..T...... C...... A...... A ..C.. [ 945] C.warreni_194521_Puerto_Plata ..T...... C...... A...... A ..C.. [ 945] D.nigropuntatus_191015_Cuba ..T...... C...... CCAT...... A....A A.--. [ 945] D.nigropunctatus_191320_Cuba ..T...... C...... CCAT...... A....A A.--. [ 945] D.nigropunctatus_191542_Cuba ..T...... C...... CCAT...... A....A ..--. [ 945] D.pleii_191574_Puerto_Rico ..T...... C...... CAT..G ....A....A A.--. [ 945] C.crusculus_101572_Content ...... C...... CA..T...... A ..C.. [ 945] C.crusculus_172442_Duncans ...... C...... A..T...... A TAC.. [ 945] C.cyanochloris_194930_Costa_Rica .....C.... G...... -...... A T.A-. [ 945] C.enneagrammus_191044_Mexico_23 .....C.... G...... -...... A T.A-. [ 945] O.apodus_194938 ...... A...... A A.A-. [ 945] C.agasepsoides_160191_Canoa AAGCACTCAA CTTACAATTG AGACACACTA GCT--ATAAC CTAGT [ 990] C.curtissi_aporus_102610_La_Descubierta ...... TC--.C...... [ 990] C.badius_194964_Navassa_Island ...... A...... TC--...... [ 990]

C.barbori_161120_Quick_Step ...... TC--.C...... [ 990] C.costatus_costatus_192054_Abricots ....G...... C--.C...... [ 990] C.darlingtoni_161687_Cacique_Enriquillo ...... G ...... TCA-T...... [ 990] C.maculatus_266527_Cayman_Brac ...... A...... TC--.C...... [ 990] C.marcanoi_193286_Valle_de_Bao ...... TC--.C...... [ 990] C.costatus_neiba_192428_Ca_Soleil ...... TC--.C...... [ 990] C.costatus_oriestes_104408_Jacmel ...... C--.C...... [ 990] C.stenurus_rugosus_103083_Nagua ...... A...... TC--...... [ 990] C.sepsoides_102369_Sabana_de_la_Mar ...... TCCC.C...... [ 990] C.warreni_172914_Hispaniola ...... G ...... TCA-T...... [ 990] C.warreni_194521_Puerto_Plata ...... G ...... TCA-T...... [ 990] D.nigropuntatus_191015_Cuba ...T.T...... ACA.T.T.. ..C----T.. ...A. [ 990] D.nigropunctatus_191320_Cuba ...T.T...... T...... ACA.T.T.. ..C----T.. ...A. [ 990] D.nigropunctatus_191542_Cuba ...T...... ACA.T.T.. ..C----T.. ...A. [ 990] D.pleii_191574_Puerto_Rico ...T.T...... C.. .ATG.T.T.. ..C----C.. ...A. [ 990] C.crusculus_101572_Content ...... TC--.C..T ..... [ 990] C.crusculus_172442_Duncans ...... TC--.C..T ..... [ 990] C.cyanochloris_194930_Costa_Rica ....TT.... T...... ACA.T...... AT--G..T ..... [ 990] C.enneagrammus_191044_Mexico_23 .-..CT...G ...... ACA.TG... .TGC--AG.T ....C [ 990] O.apodus_194938 .-...T.T...... A .A.A.TGTC. .GA---G.C. ..GAC [ 990] C.agasepsoides_160191_Canoa CTTTCTGAAC ACCCACAC-C TAGCTCAACA AAAAC-AACC ACACA [1035] C.curtissi_aporus_102610_La_Descubierta ...... TT-...... G .....-...... [1035] C.badius_194964_Navassa_Island ...... T.G..T--. ....C..GA...... -...... [1035] C.barbori_161120_Quick_Step ...... TTT. C...... -...T .T.TT [1035]

C.costatus_costatus_192054_Abricots ...... CT-...... TT...... [1035] C.darlingtoni_161687_Cacique_Enriquillo ...... T-..TA-- A...C...... -T.... .T... [1035] C.maculatus_266527_Cayman_Brac ...... C--. ....C...... -...... [1035] C.marcanoi_193286_Valle_de_Bao ...... T...TT-...... -.... .T... [1035] C.costatus_neiba_192428_Ca_Soleil ...... GTTT-...... -...... [1035] C.costatus_oriestes_104408_Jacmel ...... G.TT-...... T-...T ..G.. [1035] C.stenurus_rugosus_103083_Nagua ...... T--. ....C...... -...... [1035] C.sepsoides_102369_Sabana_de_la_Mar ...... A..C--...... -...... TT [1035] C.warreni_172914_Hispaniola ...... TG..TA-- ...... T. ....AC...A .T.T. [1035] C.warreni_194521_Puerto_Plata ...... G. ..TG..TT-- ...... T. ....AC...A .T.TG [1035] D.nigropuntatus_191015_Cuba ....A...G. .--.....-- ....C....C .CC..C.T.A .A..C [1035] D.nigropunctatus_191320_Cuba ....A...G. .--.....-- ....C....C .CT..C.T.A .A..C [1035] D.nigropunctatus_191542_Cuba ....A...G. .--.G...-- ....C....C .CT..C.T.A .A..C [1035] D.pleii_191574_Puerto_Rico ....A...G. .A-T..T.-- ....C....C ..-..C.C.A .A..T [1035] C.crusculus_101572_Content ...... A..TT-A ...... -.... .T.T. [1035] C.crusculus_172442_Duncans ...... TT-...... C....-...... [1035] C.cyanochloris_194930_Costa_Rica ....A...G. .AG.T..--- ...... A. .CC..-.C.T .T..C [1035] C.enneagrammus_191044_Mexico_23 ....A...G. C.A.T.---- ...... T. ..CT.-...T .T..T [1035] O.apodus_194938 ...... G. CTA..TC--- ...... TTC .CCCA-T..A .A... [1035] C.agasepsoides_160191_Canoa -TCCC-CCCC ATTTAAACCA AACCATTTG- --CCAAGACA AGTAA [1080] C.curtissi_aporus_102610_La_Descubierta -..TT-A..A ...-...... - --...T..T...... [1080] C.badius_194964_Navassa_Island TC..T-A...... -...... - --....A...... [1080] C.barbori_161120_Quick_Step T..T.-A...... T...... - --..C..GT...... [1080] C.costatus_costatus_192054_Abricots -..TT-A...... -...... - --...T...... [1080]

C.darlingtoni_161687_Cacique_Enriquillo T..T.-.TA. .C.-...... - --...... [1080] C.maculatus_266527_Cayman_Brac TC...-A...... -..-...... - --...... [1080] C.marcanoi_193286_Valle_de_Bao -..TT-A..A .C.-...... - --...C..T...... [1080] C.costatus_neiba_192428_Ca_Soleil -..TT-A..A G..-...... - --...T..T...... [1080] C.costatus_oriestes_104408_Jacmel -CA.T-A...... - --?..C..T...... [1080] C.stenurus_rugosus_103083_Nagua TC..T-A...... -...... - --...... [1080] C.sepsoides_102369_Sabana_de_la_Mar -..TT-A...... -...... - --...... T...... [1080] C.warreni_172914_Hispaniola T..T.-..G. .C.-G...... - --...... T...... [1080] C.warreni_194521_Puerto_Plata T..T.-..A. .C.-G...... - --...... T...... [1080] D.nigropuntatus_191015_Cuba ACTA.-AAAT ...-...... A...... G CA..C....T ..... [1080] D.nigropunctatus_191320_Cuba ACTA.-AAAT ...-...... A...... C CA..C....T ..... [1080] D.nigropunctatus_191542_Cuba ACTA.-AAAT ...-...... A...... C CA..C....T ..... [1080] D.pleii_191574_Puerto_Rico AC.-.-TAAT T..-...... A...... - ---.C....C ...T. [1080] C.crusculus_101572_Content A..T.-...... - --A.G..G...... [1080] C.crusculus_172442_Duncans TC...-A...... T...... A- --A.G..G...... [1080] C.cyanochloris_194930_Costa_Rica CCAAT--A.. CA.A-...... A...... - --....A..C ..... [1080] C.enneagrammus_191044_Mexico_23 C.AA.--.A. GA.AT...... A...... - --...... T ..... [1080] O.apodus_194938 ACTA.CAAA. CAA.T..A...... - --....AG.C ..... [1080] C.agasepsoides_160191_Canoa ATGCGATTAA ACCTCAAACC ACACTGACGC CATAGTAAAC CAGTA [1125] C.curtissi_aporus_102610_La_Descubierta ...... - ...T...... C...... [1125] C.badius_194964_Navassa_Island ...... G. ....TC.C.- ...T...... T.... [1125] C.barbori_161120_Quick_Step ...... G. ....T....- .T.T...... T....C...... [1125] C.costatus_costatus_192054_Abricots ...... - .T...... [1125] C.darlingtoni_161687_Cacique_Enriquillo ...... G. ....T--.T- .T.T...... C.... T.... [1125]

C.maculatus_266527_Cayman_Brac ...... G. ....T-.T.- .TGT...... T.... [1125] C.marcanoi_193286_Valle_de_Bao ...... - ...T...... [1125] C.costatus_neiba_192428_Ca_Soleil ...... - ...T...... - [1125] C.costatus_oriestes_104408_Jacmel ...... - ...... C...... [1125] C.stenurus_rugosus_103083_Nagua ...... G...... -.C.- ...T...... T...... T.... [1125] C.sepsoides_102369_Sabana_de_la_Mar ...... - ...T...... C...... [1125] C.warreni_172914_Hispaniola ...... --.T- ...... C.... T.... [1125] C.warreni_194521_Puerto_Plata ...... --.T- ...... C.... T.... [1125] D.nigropuntatus_191015_Cuba ...T...... G.G.- ..C...... A ....G [1125] D.nigropunctatus_191320_Cuba ...T...... G.- ..C...... A ..... [1125] D.nigropunctatus_191542_Cuba ...T...... G..- ..C...... A ..... [1125] D.pleii_191574_Puerto_Rico ...... - ..C...... C.C.A ....G [1125] C.crusculus_101572_Content ...... G...... - .T.T...... C...... [1125] C.crusculus_172442_Duncans ...... G...... - .T.T-...... C...... [1125] C.cyanochloris_194930_Costa_Rica ...... T-...A ..C.-..... A....C.C.. A.... [1125] C.enneagrammus_191044_Mexico_23 ...... -.-.A ...T-..... A....CGC.. A- ... [1125] O.apodus_194938 ...... G. .T.CT-GT.A ..---..... G...... A A- ... [1125] C.agasepsoides_160191_Canoa CCGCAGGGGA AAAATGAAAT AAAAATTC-A ACTA-TATGA AAAAA [1170] C.curtissi_aporus_102610_La_Descubierta ...T.A...... T-. ....-C...G ..... [1170] C.badius_194964_Navassa_Island .....A...... C.....-. ...GT...... [1170] C.barbori_161120_Quick_Step ...... TGA.T.. .TT.....-. ..C.-....G ..... [1170] C.costatus_costatus_192054_Abricots ...... G .TT.....-. ....-A...G ..... [1170] C.darlingtoni_161687_Cacique_Enriquillo .....-...... T.....-. ...T-A...... [1170] C.maculatus_266527_Cayman_Brac .....A...... T....T-. ...CG...... [1170]

C.marcanoi_193286_Valle_de_Bao .....A...... G .TT.....-. ....-A...... [1170] C.costatus_neiba_192428_Ca_Soleil .....A...... G .TT....A-. ....-A...... [1170] C.costatus_oriestes_104408_Jacmel ...... G .TT.....-. G...-A.... .G... [1170] C.stenurus_rugosus_103083_Nagua .....A...... T....T-. ..AT-...... [1170] C.sepsoides_102369_Sabana_de_la_Mar ...T.A...... T-. ....-C...G ..... [1170] C.warreni_172914_Hispaniola .....-...... T.....-. ....-A...... [1170] C.warreni_194521_Puerto_Plata .....-...... T.G...-. ....-A...... [1170] D.nigropuntatus_191015_Cuba .....A...... C.C...-. ..ACTACGC...... [1170] D.nigropunctatus_191320_Cuba .....A...... C.C.C.-. ..ACTACGC...... [1170] D.nigropunctatus_191542_Cuba .....A...... C.C.C.-. ..ACTACGC...... [1170] D.pleii_191574_Puerto_Rico .....A...... C.T...-T G.ATT.CGC...... [1170] C.crusculus_101572_Content .....A...... G...... TT...CT-. ....-C...G ..... [1170] C.crusculus_172442_Duncans ...... TT...CT-G ....-....G ..... [1170] C.cyanochloris_194930_Costa_Rica .....A...... TC.T.A--T ..ACTACGC. TC... [1170] C.enneagrammus_191044_Mexico_23 .....A.... .C...... CC.C.AA-T ..AC-ACGC...... [1170] O.apodus_194938 .....A...... CT.CCAACT .TA.-A.GC. .C.T. [1170] C.agasepsoides_160191_Canoa AAGCAGGAAC TAACCTCCG- TACCTCTAGC ATCATGATTT A- GCC [1215] C.curtissi_aporus_102610_La_Descubierta ...... C....C...- ...... G... .A... [1215] C.badius_194964_Navassa_Island ...... C...- ...... G... .- ... [1215] C.barbori_161120_Quick_Step ...... T A....C...- ...... T...... - ... [1215] C.costatus_costatus_192054_Abricots ...... C....C...- ...... G... .- ... [1215] C.darlingtoni_161687_Cacique_Enriquillo .-...... C...- ...... G... .- ... [1215] C.maculatus_266527_Cayman_Brac ...... C...- ...... G... .- ... [1215] C.marcanoi_193286_Valle_de_Bao .....A.G.T C....C...- ...... G... .- ... [1215]

C.costatus_neiba_192428_Ca_Soleil .-.....G.T C....C...- ...... C...... G... .- ... [1215] C.costatus_oriestes_104408_Jacmel ...... G.. C....C...- ...... C.G... .- ... [1215] C.stenurus_rugosus_103083_Nagua ...... C...- ...... G... .- ... [1215] C.sepsoides_102369_Sabana_de_la_Mar .G...... C....C...- ...... G... .- ... [1215] C.warreni_172914_Hispaniola ...... G..C...- ...... G... .- ... [1215] C.warreni_194521_Puerto_Plata ...... C...G ...... G... .- ... [1215] D.nigropuntatus_191015_Cuba C.....AC.T C..A.C...- .....T.T...... GAC. .- ... [1215] D.nigropunctatus_191320_Cuba C.....AC.T C..A.C...- .....T.T...... GAC. .- ... [1215] D.nigropunctatus_191542_Cuba C.....AC.T C..A.C...- .....T.T...... GAC. .- ... [1215] D.pleii_191574_Puerto_Rico ...... AC.T A.GA.C...- .....T.T...... GAC. .- ... [1215] C.crusculus_101572_Content ...... G.T A....C...- ...... T...... G... .- ... [1215] C.crusculus_172442_Duncans .-...... T A....C...- ...... T...... G... .- ... [1215] C.cyanochloris_194930_Costa_Rica ...... T ...T.C...- ...... C...... C...... - ... [1215] C.enneagrammus_191044_Mexico_23 ...... G.T C.TA.C...- .....T.--G ....C.G.C. .- ... [1215] O.apodus_194938 .....A.G.. .GG..C.T.- .....T.T...... G.C. .- -.. [1215] C.agasepsoides_160191_Canoa AGAAACATAC AAACAAAACG TCGTTAAAGC TTGTATCCCC GAAAT [1260] C.curtissi_aporus_102610_La_Descubierta .....T...... T. ..A...... C...... [1260] C.badius_194964_Navassa_Island ...... CTA..G...... [1260] C.barbori_161120_Quick_Step ...... C.A...... C...... [1260] C.costatus_costatus_192054_Abricots ...... T. ..A...... [1260] C.darlingtoni_161687_Cacique_Enriquillo ...... C.A...... [1260] C.maculatus_266527_Cayman_Brac ...... CTA..G...... [1260] C.marcanoi_193286_Valle_de_Bao ...... T. ..A...... A...... [1260] C.costatus_neiba_192428_Ca_Soleil ...... T. ..A...... C...... [1260]

C.costatus_oriestes_104408_Jacmel ...... T. .TA...... [1260] C.stenurus_rugosus_103083_Nagua ...... CTA..G...... [1260] C.sepsoides_102369_Sabana_de_la_Mar .....T...... T. ..A...... C...... [1260] C.warreni_172914_Hispaniola ...... C.A...... [1260] C.warreni_194521_Puerto_Plata ...... C.A...... [1260] D.nigropuntatus_191015_Cuba ..TC.ACCCA ...... A. C.A...C... C.TGTA...... [1260] D.nigropunctatus_191320_Cuba ..TC.ACCCA ...... A. C.A...C... C.TGTA...... [1260] D.nigropunctatus_191542_Cuba ..TC.ACCCA ...... A. C.A...C... C.TGTA...... [1260] D.pleii_191574_Puerto_Rico ..TC.ACCCA ...... A. C.A...T... C.T.CC...... [1260] C.crusculus_101572_Content ...... T. ..A...... [1260] C.crusculus_172442_Duncans ...... T. ..A...... [1260] C.cyanochloris_194930_Costa_Rica ...... CCG...... A. CAT.A.C... C..C.CT...... [1260] C.enneagrammus_191044_Mexico_23 ...... CCG. .G...... G. CTA...... C..CC...... C [1260] O.apodus_194938 .AGC..CCC. .GG..T.GA. ATAC..T..T C..CCC...... [1260] C.agasepsoides_160191_Canoa CAGAGTGAG- CTACTTCCTA A-GGCAGCCT AA-TAGA-GC CCACC [1305] C.curtissi_aporus_102610_La_Descubierta ...-.....- ...... T... .G...... T...G-...... [1305] C.badius_194964_Navassa_Island ...-.....- ...... T... .-...... -C...-...... [1305] C.barbori_161120_Quick_Step ...-.....- ...... T... .-...... T..AGA.. .T... [1305] C.costatus_costatus_192054_Abricots ...-.....- ...... T... --...... -....-...... [1305] C.darlingtoni_161687_Cacique_Enriquillo ...-.....- ...... T... .-...... A .TT...G-.. .A... [1305] C.maculatus_266527_Cayman_Brac ...-.....- ...... T... .-...... -C...-...... [1305] C.marcanoi_193286_Valle_de_Bao ...-A....- ...... T... --...... A....-...... [1305] C.costatus_neiba_192428_Ca_Soleil ...-.....- ...... T... --...... A....-...... [1305] C.costatus_oriestes_104408_Jacmel ...-.....- ...... T... --...... -....-...... [1305]

C.stenurus_rugosus_103083_Nagua ...-.....- ...... T... .-...... -C...-...... [1305] C.sepsoides_102369_Sabana_de_la_Mar ...-.....- ...... T... .-...... T...G-...... [1305] C.warreni_172914_Hispaniola ...-.....- ...... T.C. .-...... T. .TT-...-.. .A... [1305] C.warreni_194521_Puerto_Plata ...-.....- ...... T.C. .-...... T. .TT-...-.. .A... [1305] D.nigropuntatus_191015_Cuba ..A-.....- .....-..C. --....A.T. T---.TGA.T .A... [1305] D.nigropunctatus_191320_Cuba ..A-.....G ...... C. --....A.T. TT--.TGA.T .A... [1305] D.nigropunctatus_191542_Cuba ..A-.....- .....-..C. --....A.T. T---.TGA.T .A... [1305] D.pleii_191574_Puerto_Rico T.A-.....- .....-..C. --..T.A.A. T---.CGA.T GA... [1305] C.crusculus_101572_Content ...-.....- ...... T... .-...... T...G-...... [1305] C.crusculus_172442_Duncans ...-.....- ...... T... .-...... T...G-...... [1305] C.cyanochloris_194930_Costa_Rica ..A-.....- .....CT-C. G-.CA.C..A .T---TGGA. .A... [1305] C.enneagrammus_191044_Mexico_23 ..A-.....- .....CTTC. G-...... T---CGA.. .A... [1305] O.apodus_194938 .GA-.....- .....C--C. G-...... A .C---AGAC. .A... [1305] C.agasepsoides_160191_Canoa CATCTCTGTA GCAAAAGAGT GGGAAGACCT GCAAGTAGAG GCGAA [1350] C.curtissi_aporus_102610_La_Descubierta .G...... ------[1350] C.badius_194964_Navassa_Island .G...... GT....C .G...... [1350] C.barbori_161120_Quick_Step .G...... C...... G...... A ..... [1350] C.costatus_costatus_192054_Abricots .G...... C...... G...... [1350] C.darlingtoni_161687_Cacique_Enriquillo .G...... G...... [1350] C.maculatus_266527_Cayman_Brac .G...... GT...... G...... [1350] C.marcanoi_193286_Valle_de_Bao ...... T...... G...... [1350] C.costatus_neiba_192428_Ca_Soleil .G...... T...... G...... [1350] C.costatus_oriestes_104408_Jacmel ...... C...... G...... [1350] C.stenurus_rugosus_103083_Nagua .G...... GT....C .G...... [1350]

C.sepsoides_102369_Sabana_de_la_Mar .G...... G...... [1350] C.warreni_172914_Hispaniola .G...... G...... [1350] C.warreni_194521_Puerto_Plata .G...... G...... [1350] D.nigropuntatus_191015_Cuba .G...... CA...A .AG...... A ....C [1350] D.nigropunctatus_191320_Cuba .G...... CA...A .AG...... A ....C [1350] D.nigropunctatus_191542_Cuba .G...... CA...A .AG...... A ....C [1350] D.pleii_191574_Puerto_Rico .G...... TA...A .G...... [1350] C.crusculus_101572_Content .G...... C...... G...... A ..... [1350] C.crusculus_172442_Duncans .G...... C....C .G...... A ..... [1350] C.cyanochloris_194930_Costa_Rica .G...... C....A CAG...... [1350] C.enneagrammus_191044_Mexico_23 ...... C....C AAG...... [1350] O.apodus_194938 ...... C TG...... G. ..A.. [1350] C.agasepsoides_160191_Canoa AAACCTAACG AACCTGATGA TAGCTGGTTA CCTATTA--- - AATG [1395] C.curtissi_aporus_102610_La_Descubierta ------[1395] C.badius_194964_Navassa_Island ...... --- - .... [1395] C.barbori_161120_Quick_Step ...... --- - .... [1395] C.costatus_costatus_192054_Abricots ...... C..--- - .... [1395] C.darlingtoni_161687_Cacique_Enriquillo ...... --- - .... [1395] C.maculatus_266527_Cayman_Brac ...... C..--- - .... [1395] C.marcanoi_193286_Valle_de_Bao ...... --- - .... [1395] C.costatus_neiba_192428_Ca_Soleil ...... --- - .... [1395] C.costatus_oriestes_104408_Jacmel ...... --- - .... [1395] C.stenurus_rugosus_103083_Nagua ...... --- - .... [1395] C.sepsoides_102369_Sabana_de_la_Mar ...... G ...... --- - .... [1395]

C.warreni_172914_Hispaniola ...... --- - .... [1395] C.warreni_194521_Puerto_Plata ...... --- - .... [1395] D.nigropuntatus_191015_Cuba ...... T...... GCC.CCC A..C. [1395] D.nigropunctatus_191320_Cuba ...... T...... GCC.CCC A..C. [1395] D.nigropunctatus_191542_Cuba ...... T...... GCC.CCC A..C. [1395] D.pleii_191574_Puerto_Rico ...... T.A...... GCC.C-- A..C. [1395] C.crusculus_101572_Content ...... C..--- - .... [1395] C.crusculus_172442_Duncans ...... C..--- - .... [1395] C.cyanochloris_194930_Costa_Rica ...... T...... GA..--- - .... [1395] C.enneagrammus_191044_Mexico_23 .....A...... T..G...... G ...GA..--- - .... [1395] O.apodus_194938 ...... TC...... GA..--- - ..A. [1395] C.agasepsoides_160191_Canoa AACCTTAGTT CAATTTCAAG T-TATTAACA CAA-CCCCTA GTA-A [1440] C.curtissi_aporus_102610_La_Descubierta ------[1440] C.badius_194964_Navassa_Island ...... -...... C-T...... -. [1440] C.barbori_161120_Quick_Step ...... -...... C-...T.. ...-. [1440] C.costatus_costatus_192054_Abricots ...... -C...... CA..-A.. ...-G [1440] C.darlingtoni_161687_Cacique_Enriquillo ....C...... -C...... CA..-...... -. [1440] C.maculatus_266527_Cayman_Brac ...... -....G... ..C-...A.. ...-. [1440] C.marcanoi_193286_Valle_de_Bao .....A...... -C.....T. ..CA...A.. ...-. [1440] C.costatus_neiba_192428_Ca_Soleil .....A...... A .-C.....T. ..CA...A.. ...-. [1440] C.costatus_oriestes_104408_Jacmel ...... -C...... TA..-A.. ...-. [1440] C.stenurus_rugosus_103083_Nagua ...... -...... C-...... -. [1440] C.sepsoides_102369_Sabana_de_la_Mar ..T...... -...... A. ..C-..T...... -. [1440] C.warreni_172914_Hispaniola ...... -C.....T. ..CA..-...... -. [1440]

C.warreni_194521_Puerto_Plata ...... G...... -C.....T. ..CA..-...... -. [1440] D.nigropuntatus_191015_Cuba ..T..A.... .G.C...... C-C.C-...... A.A-...... C. [1440] D.nigropunctatus_191320_Cuba ..T..A.... .G.C...... C-C.C-...... A.A-...... C. [1440] D.nigropunctatus_191542_Cuba ..T..A.... .G.C...... C-C.C-..T. ...A.A-...... C. [1440] D.pleii_191574_Puerto_Rico ..T...... G.C...... C-..C-...... ATA-...... C. [1440] C.crusculus_101572_Content ...... -...... T. ..C-..T...... -. [1440] C.crusculus_172442_Duncans ...... -...... C-...... -. [1440] C.cyanochloris_194930_Costa_Rica ..T...... C...... CACG-AC...... A.A-... ..GCC [1440] C.enneagrammus_191044_Mexico_23 ..TT...... C...... CAC.-AC.TG ...ATA-... .C.T. [1440] O.apodus_194938 ..T...... C...... TC.CGC... ..CTAA-...... AG [1440] C.agasepsoides_160191_Canoa AT--ATTTAT A-CTTGAAAA CTATTCAATA GGGG-TACAG CCCT- [1485] C.curtissi_aporus_102610_La_Descubierta ------[1485] C.badius_194964_Navassa_Island ..--...... -...... A..-...... - [1485] C.barbori_161120_Quick_Step .C--T....C .-...... G ...... -...... - [1485] C.costatus_costatus_192054_Abricots G.--....T. .-...... A..-...... - [1485] C.darlingtoni_161687_Cacique_Enriquillo C.--.C.... .-...... G. T...... A..-...... - [1485] C.maculatus_266527_Cayman_Brac .C--...... -...... A..-...... - [1485] C.marcanoi_193286_Valle_de_Bao ..--.....G .-...... A..-...... - [1485] C.costatus_neiba_192428_Ca_Soleil ..--.....G .-...... A..-...... - [1485] C.costatus_oriestes_104408_Jacmel G.--...... -...... G A...... A..-...... - [1485] C.stenurus_rugosus_103083_Nagua ..--...... -...... A..-...... - [1485] C.sepsoides_102369_Sabana_de_la_Mar ..--....G. .-...... A..-...... - [1485] C.warreni_172914_Hispaniola .C--...... -...... -...... T..- [1485] C.warreni_194521_Puerto_Plata .C--...... -...... -...... - [1485]

D.nigropuntatus_191015_Cuba .C--T.CC.C GG...... G A...... CG ....-...... C- [1485] D.nigropunctatus_191320_Cuba .C--T.CC.C G-...... G A...... CG ....G...... CC [1485] D.nigropunctatus_191542_Cuba .C--T.CC.C G-...... G A...... CG ....-...... C- [1485] D.pleii_191574_Puerto_Rico .C--...-.. G-...... G A...... GCG ....-...... C- [1485] C.crusculus_101572_Content ..--T....C .-...... A..-...... - [1485] C.crusculus_172442_Duncans ..--T..C.. .-...... G ...... A..-...... - [1485] C.cyanochloris_194930_Costa_Rica T.CCCC...A .A...... G A...... -...... T..- [1485] C.enneagrammus_191044_Mexico_23 ----CC.C.A .A...... G A...... -...... T..- [1485] O.apodus_194938 C.--TC.GC. .G...... G A...... -...... T..- [1485] C.agasepsoides_160191_Canoa ATTGAAACAG GATACAACCT CACAAGGAGA ---ACCATAA ATCAT [1530] C.curtissi_aporus_102610_La_Descubierta ------[1530] C.badius_194964_Navassa_Island ...... G...... ---....C.. .CT.C [1530] C.barbori_161120_Quick_Step ...... ---...... G .CT.C [1530] C.costatus_costatus_192054_Abricots ...... ---....C.. .CT.C [1530] C.darlingtoni_161687_Cacique_Enriquillo ...... ---...... C..C [1530] C.maculatus_266527_Cayman_Brac ...... ---...... CTTC [1530] C.marcanoi_193286_Valle_de_Bao ...... ---....C.. TCT.C [1530] C.costatus_neiba_192428_Ca_Soleil ...... ---....C.. .C..C [1530] C.costatus_oriestes_104408_Jacmel ...... ---...... CT.C [1530] C.stenurus_rugosus_103083_Nagua ...... ---...... CT.C [1530] C.sepsoides_102369_Sabana_de_la_Mar ...... ---...... - CTTC [1530] C.warreni_172914_Hispaniola ...... T...... ---....C.. .CT.C [1530] C.warreni_194521_Puerto_Plata ...... T...... ---....C.. .CT.C [1530] D.nigropuntatus_191015_Cuba G.....C... T...... A.. AC...A.G-. ---GAACC.. CC-.C [1530]

D.nigropunctatus_191320_Cuba G.....C... T...... A.. AC...A.G.. ---GAACC.. CC..C [1530] D.nigropunctatus_191542_Cuba G.....C... T...... A.. AC...TAG.. ---GGACC.G CC-.C [1530] D.pleii_191574_Puerto_Rico GC....CT.T T...... A.. AT...A.G-. ---GAACA.. T- -.. [1530] C.crusculus_101572_Content ...... C..G..... ---...... CT.C [1530] C.crusculus_172442_Duncans ...... C...... ---...... CT.. [1530] C.cyanochloris_194930_Costa_Rica ...... T... .C.T.....C ..A.C..... --AC.A.C.. TA..C [1530] C.enneagrammus_191044_Mexico_23 ...... C...... C TT..T..... --AC.A.-.. T- .T. [1530] O.apodus_194938 ...... C... AC...... GTTGTA.... GCAC..TC.. CC..A [1530] C.agasepsoides_160191_Canoa AC--GCCACC AGT--AGGCC CTCAAGCAGC CACCA-AAAA CCCAA [1575] C.curtissi_aporus_102610_La_Descubierta ------[1575] C.badius_194964_Navassa_Island .TCAC.A..T ...--...... T..-...... T.. [1575] C.barbori_161120_Quick_Step ..CAC.A..T ...--...... T..C.... .TT.. [1575] C.costatus_costatus_192054_Abricots .TCAA.A.TT ...--...... T.T-...... T.. [1575] C.darlingtoni_161687_Cacique_Enriquillo ..CAT.A...... --G...... -G... .TT.. [1575] C.maculatus_266527_Cayman_Brac ..CAC.A..T ...--...... T..-...... T.. [1575] C.marcanoi_193286_Valle_de_Bao .TCAA.A..T ...--...... T..-.G.. TTT.. [1575] C.costatus_neiba_192428_Ca_Soleil ..CAA.A..T ...--...... T..-.G.. TTT.. [1575] C.costatus_oriestes_104408_Jacmel .TCA-.A.TT ...--...... T-.G.. .TT.. [1575] C.stenurus_rugosus_103083_Nagua ..CAC.A..T ...--...... T..-...... T.. [1575] C.sepsoides_102369_Sabana_de_la_Mar .TCAC.A...... --...... T..-...... T.. [1575] C.warreni_172914_Hispaniola ..CAT.A...... --...... G...... -.T.. TTT.. [1575] C.warreni_194521_Puerto_Plata ..CAT.A...... --...... G...... -.T.. TTT.. [1575] D.nigropuntatus_191015_Cuba .ACACA.... C..--...... A...... C...C T.-.. [1575] D.nigropunctatus_191320_Cuba .ACACA.... C.GTAG.... T.A...... C...C ..... [1575]

D.nigropunctatus_191542_Cuba .ACACA.... C..--...... A...... C...C T.-.. [1575] D.pleii_191574_Puerto_Rico .ATCTAT...... --...... A...... T..A...C T- -.. [1575] C.crusculus_101572_Content ..CAC.A..T ...--...... T..-.T.. .TT.. [1575] C.crusculus_172442_Duncans .TCAC.A..T ...--...... T..T.... .TT.. [1575] C.cyanochloris_194930_Costa_Rica ..CACA...... --....T T.A..T...... -.... TTT.. [1575] C.enneagrammus_191044_Mexico_23 C.CGTA..TT ...--G.... T.A..C...... -.C.. .AT.. [1575] O.apodus_194938 C.CAAAA.T. ...--G.... .CA...... T...-C... A.T.. [1575] C.agasepsoides_160191_Canoa AGCGTCACAG CTACCT--CA CTAAAAATAC CCAAAAAAAT -- CAA [1620] C.curtissi_aporus_102610_La_Descubierta ------[1620] C.badius_194964_Navassa_Island .....T...... --.. TC...... C...C AC... [1620] C.barbori_161120_Quick_Step G...... --.. .C...... G..C...C AC... [1620] C.costatus_costatus_192054_Abricots ...... T...... C--.. .C...... C...C AAT.. [1620] C.darlingtoni_161687_Cacique_Enriquillo ...... T.--A. .C...... G. ..T..C...C AT... [1620] C.maculatus_266527_Cayman_Brac ...... C--.. TC...... T....C...C AC... [1620] C.marcanoi_193286_Valle_de_Bao ...... T...... TC--.- .A...... T.C...C AGT.. [1620] C.costatus_neiba_192428_Ca_Soleil ...... T.. ...TAC--.- .C...... T.G.C...C AG... [1620] C.costatus_oriestes_104408_Jacmel ...... T...... AC--A. .C...... T...C...C AAT.. [1620] C.stenurus_rugosus_103083_Nagua ...... T...... --.. TC...... TT...C...C AC... [1620] C.sepsoides_102369_Sabana_de_la_Mar ...... T.--...... -...T.TTA TA.C. [1620] C.warreni_172914_Hispaniola ...... C--.. .C...... AG..C..GC CCT.. [1620] C.warreni_194521_Puerto_Plata ...... TC--.. .C...... AG..C..GC ACT.. [1620] D.nigropuntatus_191015_Cuba ...... A...ACAA. .C...... G.C...C.G AC.T. [1620] D.nigropunctatus_191320_Cuba G...... A...ACAA. .C...... A.C.CCC.C ACTT. [1620] D.nigropunctatus_191542_Cuba ...... A...ATAA. .C...... A...CCC.C AC.T. [1620]

D.pleii_191574_Puerto_Rico ...... A.T.A--AC ...... -.C..C.TC TCAT. [1620] C.crusculus_101572_Content ...... C--.. .C...... TG..C...C AC... [1620] C.crusculus_172442_Duncans ...... --T. .C...... G..C...C AC... [1620] C.cyanochloris_194930_Costa_Rica GA.....A.. .C.A-C-A.. AC...... TT .A.T..T..C - C.T. [1620] C.enneagrammus_191044_Mexico_23 ...... A.. ...T-C-A...... GTC..G..C - T.CT [1620] O.apodus_194938 G...... C.AAC-C.C AA.T....CT .A.CCTGCTC - C.CC [1620] C.agasepsoides_160191_Canoa CCTCCCTTCC CC-ACAATAG GTAATTCTAT AAT--ATAGA AGAAC [1665] C.curtissi_aporus_102610_La_Descubierta ------[1665] C.badius_194964_Navassa_Island ...... G.. -AT...... --...... [1665] C.barbori_161120_Quick_Step ...... G.. AAAT...... C--...... [1665] C.costatus_costatus_192054_Abricots ...... G.. .A-T...... --...... [1665] C.darlingtoni_161687_Cacique_Enriquillo ...... G.. TT-C...... --...... [1665] C.maculatus_266527_Cayman_Brac ...... G.. .AT...G...... C--...... [1665] C.marcanoi_193286_Valle_de_Bao ...... G.. .A-T...... C--...... [1665] C.costatus_neiba_192428_Ca_Soleil .-.....G.. .A-T...... C--...... [1665] C.costatus_oriestes_104408_Jacmel ...... A.. .A-T...... G...... C--...... [1665] C.stenurus_rugosus_103083_Nagua ...... G.. .AC...... C--...... [1665] C.sepsoides_102369_Sabana_de_la_Mar ...... CG.. .A-CT...... C--...... [1665] C.warreni_172914_Hispaniola ...... A.A .A-C....T...... C--...... [1665] C.warreni_194521_Puerto_Plata ...... A.. .A-C...... C--...... [1665] D.nigropuntatus_191015_Cuba A.-..T.A.A .A--T.CC.. ..C...... A--...... [1665] D.nigropunctatus_191320_Cuba A.-..T.A.A .A--..CC.. ..C...... A---...... [1665] D.nigropunctatus_191542_Cuba A.-..T.A.A .A--T.CC.. ..C...... G--...... [1665] D.pleii_191574_Puerto_Rico A.-..TCA.. ..--..CC.. ..C...... A--...... [1665]

C.crusculus_101572_Content ...... G.. .A-T...... C--...... [1665] C.crusculus_172442_Duncans ...... G.. .A-T...... GC--...... [1665] C.cyanochloris_194930_Costa_Rica ...T..C.AA AAC...CC...... C.. .CC--..G...... [1665] C.enneagrammus_191044_Mexico_23 ...T..C.T. TACT..CC...... CC.... .C.--..... G.... [1665] O.apodus_194938 ..CT...A.A AATTA.TC...... GCCCC...... [1665] C.agasepsoides_160191_Canoa TAATGCTAAA ATTAGTAACA AGAAATATTC TCTC-CACAC ATCTG [1710] C.curtissi_aporus_102610_La_Descubierta ------...A...... -.G...... [1710] C.badius_194964_Navassa_Island ...... A...... -TG...... [1710] C.barbori_161120_Quick_Step ...... A...... -.G...... [1710] C.costatus_costatus_192054_Abricots ..C...... A...... -.G...... [1710] C.darlingtoni_161687_Cacique_Enriquillo ...... A...... -TG...... [1710] C.maculatus_266527_Cayman_Brac ...... A...... -.G...... [1710] C.marcanoi_193286_Valle_de_Bao ..C...... A...... -.G...... [1710] C.costatus_neiba_192428_Ca_Soleil ..C...... -...... -....A...... -.G.-...... [1710] C.costatus_oriestes_104408_Jacmel ..C...... A...... -.G...... [1710] C.stenurus_rugosus_103083_Nagua ...... C...... A.C...... -.G...... [1710] C.sepsoides_102369_Sabana_de_la_Mar ...... A...... -.G...... [1710] C.warreni_172914_Hispaniola ...... A...... -.G.C...... [1710] C.warreni_194521_Puerto_Plata ...... A...... -.G...... [1710] D.nigropuntatus_191015_Cuba C...... T ...... C...... -AG..T .C..T [1710] D.nigropunctatus_191320_Cuba C...... T ...... C...... -AG..T .C..T [1710] D.nigropunctatus_191542_Cuba C...... T T...... C...... -AG..T .C..T [1710] D.pleii_191574_Puerto_Rico CC...... T ...... A...... A-AG... .C.CA [1710] C.crusculus_101572_Content C...... A...... -...... [1710]

C.crusculus_172442_Duncans C...... A-...... -...... [1710] C.cyanochloris_194930_Costa_Rica ...... TA....T G.... [1710] C.enneagrammus_191044_Mexico_23 .T...... C...... A...... T...... C... [1710] O.apodus_194938 ...... G...... CC.. CTCTCAG... .C... [1710] C.agasepsoides_160191_Canoa TAAATCAGCA ATAGAACCAC TACTGATAAT TAACCAGACC CCTA- [1755] C.curtissi_aporus_102610_La_Descubierta ...... C.C [1755] C.badius_194964_Navassa_Island ...G....A...... G..C...... A.C [1755] C.barbori_161120_Quick_Step ...... T...... C.C [1755] C.costatus_costatus_192054_Abricots ...... C.C [1755] C.darlingtoni_161687_Cacique_Enriquillo ...G...... C...... C.C [1755] C.maculatus_266527_Cayman_Brac ...... A...... C...... G.. ..A.C [1755] C.marcanoi_193286_Valle_de_Bao ...... T...C [1755] C.costatus_neiba_192428_Ca_Soleil ...... G...... T...C [1755] C.costatus_oriestes_104408_Jacmel ...... T...C [1755] C.stenurus_rugosus_103083_Nagua ...... A...... C...... G.. ..G.C [1755] C.sepsoides_102369_Sabana_de_la_Mar ...... C...... -...... C.C [1755] C.warreni_172914_Hispaniola ...... C.T [1755] C.warreni_194521_Puerto_Plata ...... C.T [1755] D.nigropuntatus_191015_Cuba ...... C...... AG.. AAA.C [1755] D.nigropunctatus_191320_Cuba ...... C...... AG.. AAA.C [1755] D.nigropunctatus_191542_Cuba ...... C.....T...... AG.. AAA.C [1755] D.pleii_191574_Puerto_Rico ...... C...... AG.. A.A.T [1755] C.crusculus_101572_Content ...... C.C [1755] C.crusculus_172442_Duncans ...... T...... C.C [1755]

C.cyanochloris_194930_Costa_Rica ..CC...... C.....TC...... C...... ---A... -- --- [1755] C.enneagrammus_191044_Mexico_23 ..CC...... C...... C...... -.A... .TAC- [1755] O.apodus_194938 ..C.....A...... AC...... C...... -..... AAA-- [1755] C.agasepsoides_160191_Canoa ----GTCTGA CTCACAAATA TACAAGCAT- -GTAGT-CTA AGCCT [1800] C.curtissi_aporus_102610_La_Descubierta CA-G...... CT.--C. CT....-..A GTA...-... .A... [1800] C.badius_194964_Navassa_Island CA-G.C...... TT.--C...... -..T A.AGTC-T.. .A... [1800] C.barbori_161120_Quick_Step TG-G...... CT..-C...... T..T AAA...-... .A... [1800] C.costatus_costatus_192054_Abricots CC-G...... CT.--C. GT....-..A GAA...-... .A... [1800] C.darlingtoni_161687_Cacique_Enriquillo CA-G...... ACT.---. C.T.G-T..- AAA...-... .A... [1800] C.maculatus_266527_Cayman_Brac CG-G...... TT..-C. CC....-..T A.AGTC-T.. .A... [1800] C.marcanoi_193286_Valle_de_Bao CC-G...... CT.--C. .T....-..A GAA...-... .A... [1800] C.costatus_neiba_192428_Ca_Soleil CC-G...... CT.--.. .T....-..A GAA...-... .A... [1800] C.costatus_oriestes_104408_Jacmel CA-G...... CT.--C. CT....-..A GAA...-... .A... [1800] C.stenurus_rugosus_103083_Nagua CG-G..G...... TT..-C...... -..T AAAGTC-T.. .A... [1800] C.sepsoides_102369_Sabana_de_la_Mar TG-G...... C..T..-C. ....G.-.CA AAAGTC-T.. .A... [1800] C.warreni_172914_Hispaniola CA-G...... AC..--C...... AT..- AAA...-... .A... [1800] C.warreni_194521_Puerto_Plata CA-G...... AC..--C. ..T..AT..- AAA...-... .A... [1800] D.nigropuntatus_191015_Cuba CAAG.AACA. ..ACACC.-. C..C.AG.CC ACA.---T.. CA.TA [1800] D.nigropunctatus_191320_Cuba CAAG.AACA. ..ACATC.-. C..C.AG.CC ACA.---T.. CA.TA [1800] D.nigropunctatus_191542_Cuba CAAG.AACA. ..ACACC... A..C.AG.CC ACA.---T.. CA.TA [1800] D.pleii_191574_Puerto_Rico TAAG..ACA. ..A--TT.C. ..TC.AGGCT AAAG---T.. .A..A [1800] C.crusculus_101572_Content TG-G...... CT..--- ...... T..T AAA...-... .A... [1800] C.crusculus_172442_Duncans TG-G...... CT..-A- ...... T.-- A.A...-... .A... [1800] C.cyanochloris_194930_Costa_Rica --GAAA...... A.T.C-C. ..AC.AG.CC CAAGTC---T .A..G [1800]

C.enneagrammus_191044_Mexico_23 -AAG.A.... TAAC..T-C. C.AC.AG.CC AAA.TC---T TA..A [1800] O.apodus_194938 -AAT.G.CC. A.ACTGC-AT C.AC.AG.CA AAA.T.AA.T .A... [1800] C.agasepsoides_160191_Canoa -CTGTTAACC CAACACAGGA GTGTAAAT-- CAGAAAGATT AAAAG [1845] C.curtissi_aporus_102610_La_Descubierta -...... C...C-A ...... [1845] C.badius_194964_Navassa_Island -...... C...C-T ...... - ..... [1845] C.barbori_161120_Quick_Step -...... C...C-A ...... [1845] C.costatus_costatus_192054_Abricots -...... C...C-A ...... [1845] C.darlingtoni_161687_Cacique_Enriquillo -...... C...CCA ...... [1845] C.maculatus_266527_Cayman_Brac -...... C...C-A ...... [1845] C.marcanoi_193286_Valle_de_Bao T...... C...C-A ...... [1845] C.costatus_neiba_192428_Ca_Soleil -...... C...C-A ...... [1845] C.costatus_oriestes_104408_Jacmel -...... C...C-A ...... [1845] C.stenurus_rugosus_103083_Nagua -...... G...... C...C-A ...... [1845] C.sepsoides_102369_Sabana_de_la_Mar -...... C...C-A ...... [1845] C.warreni_172914_Hispaniola -...... G...... C.C...CTA --...... C...... [1845] C.warreni_194521_Puerto_Plata -...... G...... C.C...CTA --...... [1845] D.nigropuntatus_191015_Cuba T...... G...... A..C..CA-- A...... [1845] D.nigropunctatus_191320_Cuba T...... G...... A..C..CA-- A...... [1845] D.nigropunctatus_191542_Cuba T...... A..C..TA-- A...... [1845] D.pleii_191574_Puerto_Rico CT...... C..TA-- A...... [1845] C.crusculus_101572_Content -...... C...C-A ...... [1845] C.crusculus_172442_Duncans -...... C-A ...... [1845] C.cyanochloris_194930_Costa_Rica CT...... A....CCA-- ...... A [1845] C.enneagrammus_191044_Mexico_23 CT...... GTA-- ...... [1845]

O.apodus_194938 A...... G...... T C..CTTTA-- -...... C.T [1845] C.agasepsoides_160191_Canoa TCAAGG--AA GGAACTCAGC AA----AAAT AT-ATCCCAA CTGTT [1890] C.curtissi_aporus_102610_La_Descubierta ...GA.--...... ----...C ..-...... [1890] C.badius_194964_Navassa_Island ....A.--.. .A.....G.. ..----...... -...... [1890] C.barbori_161120_Quick_Step ....A.--...... ----...... -...... [1890] C.costatus_costatus_192054_Abricots ...GA.--...... ----...C T.-...... G ..... [1890] C.darlingtoni_161687_Cacique_Enriquillo ...GA.--...... ----...C ..-...... [1890] C.maculatus_266527_Cayman_Brac .T..A.--...... G.. ..----...... -...... [1890] C.marcanoi_193286_Valle_de_Bao ...GA.--...... ----...... -G...... [1890] C.costatus_neiba_192428_Ca_Soleil ...GA.--...... ----...... -G...... [1890] C.costatus_oriestes_104408_Jacmel ...CA.--...... T...... ----...... -...... [1890] C.stenurus_rugosus_103083_Nagua .T..A.--...... G.. ..----...... -...... [1890] C.sepsoides_102369_Sabana_de_la_Mar ....A.--...... G.. ..----...C ..-...... [1890] C.warreni_172914_Hispaniola ....A.--...... ----..GC ..-TCG.G...... [1890] C.warreni_194521_Puerto_Plata ....A.--...... ----..GC ..-...... [1890] D.nigropuntatus_191015_Cuba ...GA.--...... G.. ..CTCA.C.C .CG.C...... [1890] D.nigropunctatus_191320_Cuba ...GA.--...... G.. ..TTCA.C.C .CG.C...... [1890] D.nigropunctatus_191542_Cuba ...GA.--...... G.. ..--CA.C.C .CG.C...... [1890] D.pleii_191574_Puerto_Rico ....A.--...... T.G C.---ACC.A .CG.C.T...... [1890] C.crusculus_101572_Content ....A.--...... ----...... -...... [1890] C.crusculus_172442_Duncans ....A.--...... ----...... -...... [1890] C.cyanochloris_194930_Costa_Rica .G..AA--.G A------[1890] C.enneagrammus_191044_Mexico_23 .T...A--...... T...... ----.TTC TCA.C...... [1890] O.apodus_194938 .A...A--.G .-...... G ..----.CTA GCGTC------[1890]

C.agasepsoides_160191_Canoa TACCAAAAAC ATAGCCTTTA GCCAAAAATA TTAAAGGTCC CGCCT [1935] C.curtissi_aporus_102610_La_Descubierta ...... [1935] C.badius_194964_Navassa_Island ...... T...... [1935] C.barbori_161120_Quick_Step ...... [1935] C.costatus_costatus_192054_Abricots ...... [1935] C.darlingtoni_161687_Cacique_Enriquillo ...... A... [1935] C.maculatus_266527_Cayman_Brac ...... T...... [1935] C.marcanoi_193286_Valle_de_Bao ...... [1935] C.costatus_neiba_192428_Ca_Soleil ...... G...... [1935] C.costatus_oriestes_104408_Jacmel ...... [1935] C.stenurus_rugosus_103083_Nagua ...... T...... [1935] C.sepsoides_102369_Sabana_de_la_Mar ...... [1935] C.warreni_172914_Hispaniola ...... [1935] C.warreni_194521_Puerto_Plata ...... [1935] D.nigropuntatus_191015_Cuba ...... A..C...... [1935] D.nigropunctatus_191320_Cuba ...... A..C...... [1935] D.nigropunctatus_191542_Cuba ...... A..C...... [1935] D.pleii_191574_Puerto_Rico ...... AT.C...... T.... [1935] C.crusculus_101572_Content ...... C...... [1935] C.crusculus_172442_Duncans ...... T...... [1935] C.cyanochloris_194930_Costa_Rica ------[1935] C.enneagrammus_191044_Mexico_23 ...... TTT...... [1935] O.apodus_194938 ------[1935]

C.agasepsoides_160191_Canoa GCCCAGT-GA CACTACA-CG TTAAACGGCC GCGGTATACT AACCG [1980] C.curtissi_aporus_102610_La_Descubierta ...... -.. .G.CCA--...... [1980] C.badius_194964_Navassa_Island ...... -.. ...C...-...... [1980] C.barbori_161120_Quick_Step ...... -.. ...C...-...... [1980] C.costatus_costatus_192054_Abricots ...... -.. .C.CTA.-...... [1980] C.darlingtoni_161687_Cacique_Enriquillo ...... -.. ...CCA.-...... [1980] C.maculatus_266527_Cayman_Brac ...... -.. ...C...-...... [1980] C.marcanoi_193286_Valle_de_Bao ...... -.. ...CCA.-...... [1980] C.costatus_neiba_192428_Ca_Soleil ...... T.. ...CCA.-...... [1980] C.costatus_oriestes_104408_Jacmel ...... -.. ...CCA--...... [1980] C.stenurus_rugosus_103083_Nagua ...... -.. ...C..G-...... [1980] C.sepsoides_102369_Sabana_de_la_Mar ...... -.. ...CCA--...... [1980] C.warreni_172914_Hispaniola ...... -.. ...CC..-...... [1980] C.warreni_194521_Puerto_Plata ...... -.. ...CC..-...... [1980] D.nigropuntatus_191015_Cuba ...... -...... ACC...... [1980] D.nigropunctatus_191320_Cuba ...... -...... ACC...... [1980] D.nigropunctatus_191542_Cuba ...... -...... ACC...... [1980] D.pleii_191574_Puerto_Rico ...... -.. ...C.----. ..T...... [1980] C.crusculus_101572_Content ...... -.. ...CT..-...... [1980] C.crusculus_172442_Duncans ...... -.. ...CC..-...... [1980] C.cyanochloris_194930_Costa_Rica ------[1980] C.enneagrammus_191044_Mexico_23 ...... --- -GAC.A.CT...... [1980] O.apodus_194938 ------[1980]

C.agasepsoides_160191_Canoa TGCAAAGGTA GCGTAATCAC TTGTCTCCTA AATAGAGACC AGTAT [2025] C.curtissi_aporus_102610_La_Descubierta ...... [2025] C.badius_194964_Navassa_Island ...... [2025] C.barbori_161120_Quick_Step ...... [2025] C.costatus_costatus_192054_Abricots ...... [2025] C.darlingtoni_161687_Cacique_Enriquillo ...... [2025] C.maculatus_266527_Cayman_Brac ...... [2025] C.marcanoi_193286_Valle_de_Bao ...... [2025] C.costatus_neiba_192428_Ca_Soleil ...... [2025] C.costatus_oriestes_104408_Jacmel ...... [2025] C.stenurus_rugosus_103083_Nagua ...... [2025] C.sepsoides_102369_Sabana_de_la_Mar ...... [2025] C.warreni_172914_Hispaniola ...... T ..... [2025] C.warreni_194521_Puerto_Plata ...... ?...... T ..... [2025] D.nigropuntatus_191015_Cuba ...... C...... C...T C.... [2025] D.nigropunctatus_191320_Cuba ...... C...... C...T C.... [2025] D.nigropunctatus_191542_Cuba ...... C...... C...T C.... [2025] D.pleii_191574_Puerto_Rico ...... C...... G...T C.... [2025] C.crusculus_101572_Content ...... [2025] C.crusculus_172442_Duncans ...... [2025] C.cyanochloris_194930_Costa_Rica ------[2025] C.enneagrammus_191044_Mexico_23 ...... C...... T ..... [2025] O.apodus_194938 ------[2025]

C.agasepsoides_160191_Canoa GAACGGCTAA ATGAGGATAA AACTGTCTCC CAAGACTAAT CAGTA [2070] C.curtissi_aporus_102610_La_Descubierta ...T...... [2070] C.badius_194964_Navassa_Island ...T...... T...... [2070] C.barbori_161120_Quick_Step ...T...... G...... [2070] C.costatus_costatus_192054_Abricots ...T...... T...... [2070] C.darlingtoni_161687_Cacique_Enriquillo ...T..T...... [2070] C.maculatus_266527_Cayman_Brac ...T...... T...... [2070] C.marcanoi_193286_Valle_de_Bao ...T...... T...... [2070] C.costatus_neiba_192428_Ca_Soleil ...T...... [2070] C.costatus_oriestes_104408_Jacmel ...T...... TT...... G [2070] C.stenurus_rugosus_103083_Nagua ...... T.A...... G [2070] C.sepsoides_102369_Sabana_de_la_Mar ...... G...... [2070] C.warreni_172914_Hispaniola ...T...... [2070] C.warreni_194521_Puerto_Plata ...T...... [2070] D.nigropuntatus_191015_Cuba ...... G.CC .T...... CT...C... T...G [2070] D.nigropunctatus_191320_Cuba ...... G.CC .T...... CC...C...... G [2070] D.nigropunctatus_191542_Cuba ...... G.CC .T...... CT...C...... G [2070] D.pleii_191574_Puerto_Rico ...... G...... A.GCC. .C...... T.T...C...... G [2070] C.crusculus_101572_Content ...T...... [2070] C.crusculus_172442_Duncans ...T...... [2070] C.cyanochloris_194930_Costa_Rica ------[2070] C.enneagrammus_191044_Mexico_23 ...T...... G..C ...... T .T.A..C...... G [2070] O.apodus_194938 ------[2070]

C.agasepsoides_160191_Canoa AAA-TTGATC TACCAGTACA AAAGCTGGCA TAGACCCATA AGACG [2115] C.curtissi_aporus_102610_La_Descubierta ...-...... A...... [2115] C.badius_194964_Navassa_Island ...-...... A...... [2115] C.barbori_161120_Quick_Step ...-...... G...... A...... [2115] C.costatus_costatus_192054_Abricots ...-...... A...... [2115] C.darlingtoni_161687_Cacique_Enriquillo ...-...... T. ..T...... [2115] C.maculatus_266527_Cayman_Brac ...-...... A...... [2115] C.marcanoi_193286_Valle_de_Bao ...-...... A...... [2115] C.costatus_neiba_192428_Ca_Soleil ...-...... A...... [2115] C.costatus_oriestes_104408_Jacmel ...-...... TT.G...... A...... [2115] C.stenurus_rugosus_103083_Nagua ...-C...... A...... [2115] C.sepsoides_102369_Sabana_de_la_Mar ...-...... A...... [2115] C.warreni_172914_Hispaniola ...-...... A...... [2115] C.warreni_194521_Puerto_Plata ...-...... A...... [2115] D.nigropuntatus_191015_Cuba ...AC...... T.G...... T. ..A...... [2115] D.nigropunctatus_191320_Cuba ...-...... T...... G...T. ..A...... [2115] D.nigropunctatus_191542_Cuba ...-....A...... T. ..A...... [2115] D.pleii_191574_Puerto_Rico ...-....A. ..T...... T. ..A...... [2115] C.crusculus_101572_Content ...-...... A...... [2115] C.crusculus_172442_Duncans ...-...... A...... [2115] C.cyanochloris_194930_Costa_Rica ------[2115] C.enneagrammus_191044_Mexico_23 ...-.....T ...... T. ..CC...... [2115] O.apodus_194938 ------[2115]

C.agasepsoides_160191_Canoa AGAAGACCCT GTGGAGCTTT AGATACCCGC CAACC----A ACCAT [2160] C.curtissi_aporus_102610_La_Descubierta ...... TAG.. ...TT----...... [2160] C.badius_194964_Navassa_Island ...... C .....TA.A...... ----...... [2160] C.barbori_161120_Quick_Step ...... A.A. ...A.----...... [2160] C.costatus_costatus_192054_Abricots ...... C ...... A...... TT----...... [2160] C.darlingtoni_161687_Cacique_Enriquillo ...... TA.A...... ----...... [2160] C.maculatus_266527_Cayman_Brac ...... C ...... A.A...... ----...... [2160] C.marcanoi_193286_Valle_de_Bao ...... AG.. ...TT----...... [2160] C.costatus_neiba_192428_Ca_Soleil ...... TAG.. ...TT----...... [2160] C.costatus_oriestes_104408_Jacmel ...... C ...... A...... TT----...... [2160] C.stenurus_rugosus_103083_Nagua ...... C ...... A.A...... ----...... [2160] C.sepsoides_102369_Sabana_de_la_Mar ...... C .....TA...... T----. ..-.. [2160] C.warreni_172914_Hispaniola ...... A.A...... ----...... [2160] C.warreni_194521_Puerto_Plata ...... C ...... A...... TT----...... [2160] D.nigropuntatus_191015_Cuba ...... A.....C .A..G.A.A...... CCTAC ..... [2160] D.nigropunctatus_191320_Cuba ...... A.....C .A..G.A.A...... CCTAC ..... [2160] D.nigropunctatus_191542_Cuba ...... A...... A..G.A.A...... CCTAC ..... [2160] D.pleii_191574_Puerto_Rico ...... A...AGTAT ...T.CACC. C.... [2160] C.crusculus_101572_Content ...... A...... A.----. .T... [2160] C.crusculus_172442_Duncans ...... TA.A. ...A------.T... [2160] C.cyanochloris_194930_Costa_Rica ------[2160] C.enneagrammus_191044_Mexico_23 ...... A ....GT..A. ...A.CCAAC C.T.C [2160] O.apodus_194938 ------[2160]

C.agasepsoides_160191_Canoa GGCACACTAT CTTTAG--TT GGGGCGACTA CGGAAAC-AA ACAAA [2205] C.curtissi_aporus_102610_La_Descubierta ...... A...... --...... G ...... -...... [2205] C.badius_194964_Navassa_Island ...... --...... -...... [2205] C.barbori_161120_Quick_Step ...... --...... -...... [2205] C.costatus_costatus_192054_Abricots ..T...... --...... -...... [2205] C.darlingtoni_161687_Cacique_Enriquillo ..T.A...... --...... -...... [2205] C.maculatus_266527_Cayman_Brac ...... --...... T-...... [2205] C.marcanoi_193286_Valle_de_Bao ...... A...... --...... -...... [2205] C.costatus_neiba_192428_Ca_Soleil ...... A...... --...... -...... [2205] C.costatus_oriestes_104408_Jacmel ..T...... --...... -...... [2205] C.stenurus_rugosus_103083_Nagua ...... --...... -...... [2205] C.sepsoides_102369_Sabana_de_la_Mar ...-T...... --...... G ...... -...... [2205] C.warreni_172914_Hispaniola ...... --...... -...... [2205] C.warreni_194521_Puerto_Plata ..T...... --...... -...... [2205] D.nigropuntatus_191015_Cuba ..AC...C.. T...TAGT...... -.. ..C.. [2205] D.nigropunctatus_191320_Cuba ..AC...C.. T.....--...... -.. ..C.. [2205] D.nigropunctatus_191542_Cuba ..GC...C.. T.....--...... -.. ..C.. [2205] D.pleii_191574_Puerto_Rico .AAC...C.. T.....--...... T-...... [2205] C.crusculus_101572_Content ...... --...... C...... [2205] C.crusculus_172442_Duncans ...... --...... G..-...... [2205] C.cyanochloris_194930_Costa_Rica ------[2205] C.enneagrammus_191044_Mexico_23 TATGGT.C.C .A.CTTCAG. T...GCGACT AC.G.GAC...... [2205] O.apodus_194938 ------[2205]

C.agasepsoides_160191_Canoa ACTTCCAAGC AAGGGAAA-- --CCCCCCAA AGACAAACAT GTCGA [2250] C.curtissi_aporus_102610_La_Descubierta ...... A ..A..G..-C CC....AA...... G...... [2250] C.badius_194964_Navassa_Island ...... A.....-C CTAT...... G....C ..... [2250] C.barbori_161120_Quick_Step ...... A.....-A TTAT...... G....C ..... [2250] C.costatus_costatus_192054_Abricots ...... A.....-C CCAT..AA...... G...... [2250] C.darlingtoni_161687_Cacique_Enriquillo ...... C.A..G..AT TTA....A...... G...C ..... [2250] C.maculatus_266527_Cayman_Brac ...... A.....-C CCAT...... C....C ..... [2250] C.marcanoi_193286_Valle_de_Bao ...... A ..A.....-C CCA...AA...... T...... [2250] C.costatus_neiba_192428_Ca_Soleil ...... A ..A.....-C CGA...AA...... [2250] C.costatus_oriestes_104408_Jacmel ...... A.....-C CCA...AA...... G...... [2250] C.stenurus_rugosus_103083_Nagua ...... A.....-C C-AT.....T ....G....C ..... [2250] C.sepsoides_102369_Sabana_de_la_Mar ...... A.....AC CCAT...T...... G...GC ..... [2250] C.warreni_172914_Hispaniola ...... A.G..AT TTA.....C...... G...C ..... [2250] C.warreni_194521_Puerto_Plata ...... -A.GG.AC CCA...AA...... G...... [2250] D.nigropuntatus_191015_Cuba ...... A ..A--.C.------..A.-. GA-.CC...... T. [2250] D.nigropunctatus_191320_Cuba ...... A ..A--.C.------..A.-. GA..CC...... T. [2250] D.nigropunctatus_191542_Cuba ...... A ..A--.C.------..A.-. GA-.CC...... T. [2250] D.pleii_191574_Puerto_Rico ...... C.AT-...------..A.C. ....TC...... T. [2250] C.crusculus_101572_Content ...... A.....-A TTAT...... G...... [2250] C.crusculus_172442_Duncans ...... A.....-A TTAT...... T....C ..... [2250] C.cyanochloris_194930_Costa_Rica ------[2250] C.enneagrammus_191044_Mexico_23 ..C...C... C..A...CAC TA.T.TG..C ....CC...... [2250] O.apodus_194938 ------[2250]

C.agasepsoides_160191_Canoa CACACAGACC CAGTA-CTAC TGAAAAA-CG AACCAA-GTT ACCCC [2295] C.curtissi_aporus_102610_La_Descubierta ...... C...-...... T...-...... -...... [2295] C.badius_194964_Navassa_Island ...... -T...... -...... -...... [2295] C.barbori_161120_Quick_Step ...... -...... G....-...... -...... [2295] C.costatus_costatus_192054_Abricots ...... -A...... -...... -...... [2295] C.darlingtoni_161687_Cacique_Enriquillo .G...... -.C...... -...... -...... [2295] C.maculatus_266527_Cayman_Brac ...... -T...... -...... -...... [2295] C.marcanoi_193286_Valle_de_Bao ...... -.A.. ...T...-...... -...... [2295] C.costatus_neiba_192428_Ca_Soleil ..A...... -...... T...-...... -...... [2295] C.costatus_oriestes_104408_Jacmel ...... -...... G..T-...... -...... [2295] C.stenurus_rugosus_103083_Nagua ...... -...... -...... -...... [2295] C.sepsoides_102369_Sabana_de_la_Mar A...... -.C.. ...T...-...... -...... [2295] C.warreni_172914_Hispaniola ...... -...... -...... G-...... [2295] C.warreni_194521_Puerto_Plata ...... -...... G...-...... -...... [2295] D.nigropuntatus_191015_Cuba AC..TC...... G.C...... T..A...... T... ..T.. [2295] D.nigropunctatus_191320_Cuba AC..TC...... -.C...... T..-...... -... ..T.. [2295] D.nigropunctatus_191542_Cuba AC..TC...... -.C...... T..-...... -... ..T.. [2295] D.pleii_191574_Puerto_Rico ACT..C..T...... -.C...... T..-...... -...... [2295] C.crusculus_101572_Content ...... -.A.. ..GG...-...... -...... [2295] C.crusculus_172442_Duncans ...... -...... G....-...... -...... [2295] C.cyanochloris_194930_Costa_Rica ------[2295] C.enneagrammus_191044_Mexico_23 ....AC...... -.A.. ...GT..-...... -...... [2295] O.apodus_194938 ------[2295]

C.agasepsoides_160191_Canoa AGGGATAACA -GCGCTATCT TCTTCTAGAG TCCCCATCGC CCAGA [2340] C.curtissi_aporus_102610_La_Descubierta ...... -...... A .A... [2340] C.badius_194964_Navassa_Island ...... -....C...... A...... A... [2340] C.barbori_161120_Quick_Step ...... -...... A... [2340] C.costatus_costatus_192054_Abricots ...... -...... A .A... [2340] C.darlingtoni_161687_Cacique_Enriquillo ...... -...... [2340] C.maculatus_266527_Cayman_Brac ...... -....C...... A...... A... [2340] C.marcanoi_193286_Valle_de_Bao ...... -...... A .A... [2340] C.costatus_neiba_192428_Ca_Soleil ...... -...... A .A... [2340] C.costatus_oriestes_104408_Jacmel ...... -...... A .A... [2340] C.stenurus_rugosus_103083_Nagua ...... -....C...... A...... A... [2340] C.sepsoides_102369_Sabana_de_la_Mar ...... -...... A... [2340] C.warreni_172914_Hispaniola ...... -...... A... [2340] C.warreni_194521_Puerto_Plata ...... -...... A .A... [2340] D.nigropuntatus_191015_Cuba ...... T...... A... [2340] D.nigropunctatus_191320_Cuba ...... -...... A... [2340] D.nigropunctatus_191542_Cuba ...... -...... A... [2340] D.pleii_191574_Puerto_Rico ...... -...... TC...... A... [2340] C.crusculus_101572_Content ...... -...... -...... A... [2340] C.crusculus_172442_Duncans ...... -...... A... [2340] C.cyanochloris_194930_Costa_Rica ------[2340] C.enneagrammus_191044_Mexico_23 ...... -....A...... A... [2340] O.apodus_194938 ------[2340]

C.agasepsoides_160191_Canoa AGGTTTACGA CC-TCGA-TG TTGG-ATCAG GATACCCCA- TGGTG [2385] C.curtissi_aporus_102610_La_Descubierta ...... -....-...... G...... A ..... [2385] C.badius_194964_Navassa_Island ...... -....-...... -...... A ..... [2385] C.barbori_161120_Quick_Step ...... -....-.. .-..-...... A ..... [2385] C.costatus_costatus_192054_Abricots ...... -....-.. .-..-...... A ..... [2385] C.darlingtoni_161687_Cacique_Enriquillo ...... -..C.-...... -...... A.A ..... [2385] C.maculatus_266527_Cayman_Brac ...... -....-...... -...... A ..... [2385] C.marcanoi_193286_Valle_de_Bao ...... -....-...... -...... A ..... [2385] C.costatus_neiba_192428_Ca_Soleil ...... -....-...... -...... A ..... [2385] C.costatus_oriestes_104408_Jacmel ...... -....-...... -...... A ..... [2385] C.stenurus_rugosus_103083_Nagua ...... -....-...... -...... A ..... [2385] C.sepsoides_102369_Sabana_de_la_Mar ...... -....-...... -...... - ..... [2385] C.warreni_172914_Hispaniola ...... -....-...... -...... A.A ..... [2385] C.warreni_194521_Puerto_Plata ...... -....-...... -...... A ..... [2385] D.nigropuntatus_191015_Cuba ...... G.C....-...... -...... C....A.A ...C. [2385] D.nigropunctatus_191320_Cuba ...... -.C....-...... -...... C....A.A ...C. [2385] D.nigropunctatus_191542_Cuba ...... -.C....-...... -...... C....A.A ...C. [2385] D.pleii_191574_Puerto_Rico ...... -.C....-...... -...... C....A.A ...C. [2385] C.crusculus_101572_Content ...... -....-...... -...... A.A ..... [2385] C.crusculus_172442_Duncans ...... C....C...... -...... A ..... [2385] C.cyanochloris_194930_Costa_Rica ------[2385] C.enneagrammus_191044_Mexico_23 ...... --TCGA...... -...... C...... - ..... [2385] O.apodus_194938 ------[2385]

C.agasepsoides_160191_Canoa CAGCCCCTAT TAAAGGCTCG TTTGTTCACC GATTA--AAT CCTAC [2430] C.curtissi_aporus_102610_La_Descubierta .....A...... T...... A...... AT...... [2430] C.badius_194964_Navassa_Island .....A...... T...... A...... AT...... [2430] C.barbori_161120_Quick_Step .....A...... T...... A...... AT...... [2430] C.costatus_costatus_192054_Abricots .....A...... T...... A...... AT...... [2430] C.darlingtoni_161687_Cacique_Enriquillo ...... T...... -...... AC...... [2430] C.maculatus_266527_Cayman_Brac .....A...... T...... A...... AT...... [2430] C.marcanoi_193286_Valle_de_Bao .....A...... -..T...... -...... AT...... [2430] C.costatus_neiba_192428_Ca_Soleil .....A...... T...... A...... AT...... [2430] C.costatus_oriestes_104408_Jacmel .....A...... T...... A...... AT...... [2430] C.stenurus_rugosus_103083_Nagua .....A...... T... .C...... A...... AT...... [2430] C.sepsoides_102369_Sabana_de_la_Mar ...... -..T...... A...... AC...... [2430] C.warreni_172914_Hispaniola .....A.... .T....T...... A...... AC...... [2430] C.warreni_194521_Puerto_Plata .....A...... T...... A...... AT...... [2430] D.nigropuntatus_191015_Cuba .....A...... T...... A...... ATG-...... [2430] D.nigropunctatus_191320_Cuba .....A...... T...... A...... ATG-...... [2430] D.nigropunctatus_191542_Cuba ...... T...... A...... AGG-...... [2430] D.pleii_191574_Puerto_Rico .....A...... T...... A...... ATG-...... [2430] C.crusculus_101572_Content .....A...... T... ..G--...A...... AT...... [2430] C.crusculus_172442_Duncans .....A...... T...... A...... AT...... [2430] C.cyanochloris_194930_Costa_Rica ------[2430] C.enneagrammus_191044_Mexico_23 .....A...... T...... A...... AC.G...... [2430] O.apodus_194938 ------[2430]

C.agasepsoides_160191_Canoa GTGATCTGAG TTCAGACCGG AGAAATCCAG GTCGGTTTCT ATCTA [2475] C.curtissi_aporus_102610_La_Descubierta ...... [2475] C.badius_194964_Navassa_Island ...... A...... [2475] C.barbori_161120_Quick_Step ...... [2475] C.costatus_costatus_192054_Abricots ...... [2475] C.darlingtoni_161687_Cacique_Enriquillo C...... [2475] C.maculatus_266527_Cayman_Brac ...... [2475] C.marcanoi_193286_Valle_de_Bao ...... [2475] C.costatus_neiba_192428_Ca_Soleil ...... [2475] C.costatus_oriestes_104408_Jacmel ...... [2475] C.stenurus_rugosus_103083_Nagua ...... [2475] C.sepsoides_102369_Sabana_de_la_Mar ...... [2475] C.warreni_172914_Hispaniola ...... [2475] C.warreni_194521_Puerto_Plata ...... [2475] D.nigropuntatus_191015_Cuba ...... [2475] D.nigropunctatus_191320_Cuba ...... [2475] D.nigropunctatus_191542_Cuba ...... [2475] D.pleii_191574_Puerto_Rico ...... [2475] C.crusculus_101572_Content ...... [2475] C.crusculus_172442_Duncans ...... [2475] C.cyanochloris_194930_Costa_Rica ------[2475] C.enneagrammus_191044_Mexico_23 ...... [2475] O.apodus_194938 ------[2475]

C.agasepsoides_160191_Canoa TGAAAAGTTT TCTTCAGTAC GAAAGGACCA TGAAAACAGG ACCAA [2520] C.curtissi_aporus_102610_La_Descubierta ...... G...... [2520] C.badius_194964_Navassa_Island ...... [2520] C.barbori_161120_Quick_Step ...... [2520] C.costatus_costatus_192054_Abricots ...... G...... [2520] C.darlingtoni_161687_Cacique_Enriquillo ...... [2520] C.maculatus_266527_Cayman_Brac ...... [2520] C.marcanoi_193286_Valle_de_Bao ....T...... G...... [2520] C.costatus_neiba_192428_Ca_Soleil ...... G...... [2520] C.costatus_oriestes_104408_Jacmel ...... G...... [2520] C.stenurus_rugosus_103083_Nagua ...... [2520] C.sepsoides_102369_Sabana_de_la_Mar ...... A...... [2520] C.warreni_172914_Hispaniola ...... [2520] C.warreni_194521_Puerto_Plata ...... G...... [2520] D.nigropuntatus_191015_Cuba ..TT..A...... C...... A.....T... .T.C. [2520] D.nigropunctatus_191320_Cuba ..TT..A...... C...... A.....TG.. .T.C. [2520] D.nigropunctatus_191542_Cuba ..TT..A...... A.....T... .T.C. [2520] D.pleii_191574_Puerto_Rico ..TT..A...... A.....T... .T.T. [2520] C.crusculus_101572_Content ...... [2520] C.crusculus_172442_Duncans ...... [2520] C.cyanochloris_194930_Costa_Rica ------[2520] C.enneagrammus_191044_Mexico_23 ...T..A...... A.....T... .T.C. [2520] O.apodus_194938 ------[2520]

C.agasepsoides_160191_Canoa CCCCACCAGA ACGTCCTTCC CAATATAGCT GACACA-TCT CAAAC [2565] C.curtissi_aporus_102610_La_Descubierta TT.T.A...... C...... A.-.CCTC T.... [2565] C.badius_194964_Navassa_Island TA...... A...... C...... -...... [2565] C.barbori_161120_Quick_Step TA.T...... C...... T.T.-...... [2565] C.costatus_costatus_192054_Abricots T..T.A...... C...... A...--TC TT... [2565] C.darlingtoni_161687_Cacique_Enriquillo T...... C...... C..--TC T.... [2565] C.maculatus_266527_Cayman_Brac TAT.....A...... C...... -...... G. [2565] C.marcanoi_193286_Valle_de_Bao T..T.A...... C...... AG-GCCTC T.... [2565] C.costatus_neiba_192428_Ca_Soleil T..T.A...... C...... A.-.CCTC T.... [2565] C.costatus_oriestes_104408_Jacmel T..T.A...... C...... A.T.AC.C T.... [2565] C.stenurus_rugosus_103083_Nagua TAT.....A...... GC...... -...... G. [2565] C.sepsoides_102369_Sabana_de_la_Mar TA.T...... C..G.T. ..T...-... A.... [2565] C.warreni_172914_Hispaniola TT.T...... C....G. ....T.--TC TG... [2565] C.warreni_194521_Puerto_Plata T..T.A...... C...... A.T.AC.C T.... [2565] D.nigropuntatus_191015_Cuba T.TA...CAG ..A.....GT G.CAG...... A-.. ...C. [2565] D.nigropunctatus_191320_Cuba T.TA...CAG ..A.....GT G.CAG...... A-.. ...C. [2565] D.nigropunctatus_191542_Cuba T.TA...CAG ..A.....GT A.CAG...... A-.. ...C. [2565] D.pleii_191574_Puerto_Rico T.TA...TAG ..A....CA. -.CA...... T.AA.. ...C. [2565] C.crusculus_101572_Content T...... G ...... CC...... T.T.-...... [2565] C.crusculus_172442_Duncans T..A.....G ...... C...... T.-...... [2565] C.cyanochloris_194930_Costa_Rica ------[2565] C.enneagrammus_191044_Mexico_23 T.TT.AA.AG ..A....A.. .C.C..G..G ....T.AC.C A.--- [2565] O.apodus_194938 ------[2565]

C.agasepsoides_160191_Canoa CAAAA-ACGG GACCATTAAC CCCCAGCCCC AGACAAGGGC TAA-T [2610] C.curtissi_aporus_102610_La_Descubierta .....-...... ATACT-- -...... T ...... -. [2610] C.badius_194964_Navassa_Island .G...-.T.. ...ACACT.A -...... T ...... -. [2610] C.barbori_161120_Quick_Step .....-...... ACACT-- -...GA...T ...... T ...-. [2610] C.costatus_costatus_192054_Abricots .....-...... ATACT-- -...... T ...... ------[2610] C.darlingtoni_161687_Cacique_Enriquillo .....-...... ACCC.-- ..A..A...T ...... T .G.A. [2610] C.maculatus_266527_Cayman_Brac .G...-.T.. ...ACA.T.A T...... T ...... T ...-. [2610] C.marcanoi_193286_Valle_de_Bao .....-...... AT.CT-- -...... T ...... -. [2610] C.costatus_neiba_192428_Ca_Soleil .....-...... ATACT-- -...... T ...... -. [2610] C.costatus_oriestes_104408_Jacmel .....-...... ATACT-- -...... T ...... T.-. [2610] C.stenurus_rugosus_103083_Nagua .....-.T.. ...ACA.T.A T...... T ...... -. [2610] C.sepsoides_102369_Sabana_de_la_Mar .....-...... ACCC.-- -....A...A ....T....T ...A. [2610] C.warreni_172914_Hispaniola ...T.-...... ACCC.-- ..A..A...... T .- .A. [2610] C.warreni_194521_Puerto_Plata .....-...... AT--.-- .T...... T ...... - .A. [2610] D.nigropuntatus_191015_Cuba T.CC.C..CA C.AAC-C..T AAATCA.T.G ...T...A.- - TTT. [2610] D.nigropunctatus_191320_Cuba T.CC.C..CA C.GAC-C... AAA.CA.T.G ...T...A.- - TTT. [2610] D.nigropunctatus_191542_Cuba T.CC.C..CA C.AAC-C..T AAA.CA.T.G .A.T...A.- - TTT. [2610] D.pleii_191574_Puerto_Rico T.T..C..TA CCAATAC... TAA--A.T.G .A.....A.. ATTT. [2610] C.crusculus_101572_Content -....-.... A..ACACT-- -...... T ...... -. [2610] C.crusculus_172442_Duncans -....-...... ACA.T-- -...... T ...... -. [2610] C.cyanochloris_194930_Costa_Rica ------[2610] C.enneagrammus_191044_Mexico_23 ------[2610] O.apodus_194938 ------[2610]

C.agasepsoides_160191_Canoa TAGGGTGGCA GAGCTAGGCT -AATGCAAAA GGCCTAA [2647] C.curtissi_aporus_102610_La_Descubierta ...... T. T...... ------[2647] C.badius_194964_Navassa_Island ...... T...... [2647] C.barbori_161120_Quick_Step ...... T. T...... [2647] C.costatus_costatus_192054_Abricots ------[2647] C.darlingtoni_161687_Cacique_Enriquillo ...... T. T...... [2647] C.maculatus_266527_Cayman_Brac .....-...... T. T.------[2647] C.marcanoi_193286_Valle_de_Bao ...... T. T...... [2647] C.costatus_neiba_192428_Ca_Soleil ...... T. T...... ------[2647] C.costatus_oriestes_104408_Jacmel ...... T. T...... [2647] C.stenurus_rugosus_103083_Nagua ...... T. T...... [2647] C.sepsoides_102369_Sabana_de_la_Mar ...... T. T...... [2647] C.warreni_172914_Hispaniola ...... C...T. T...... ------[2647] C.warreni_194521_Puerto_Plata ...... T. T...... [2647] D.nigropuntatus_191015_Cuba -...... C...T. --...... [2647] D.nigropunctatus_191320_Cuba -....G.CG. ....C...G. --...... [2647] D.nigropunctatus_191542_Cuba ...... C...T. --...... [2647] D.pleii_191574_Puerto_Rico ...... C...T. T-...... [2647] C.crusculus_101572_Content ...... T. T...... [2647] C.crusculus_172442_Duncans ...... T. T...... [2647] C.cyanochloris_194930_Costa_Rica ------[2647] C.enneagrammus_191044_Mexico_23 ------[2647] O.apodus_194938 ------[2647]

APPENDIX D. ND2 mtDNA SEQUENCE DATA.

C.agasepsoides_160191_Canoa TAACCATTCT AATAACCAAC CTAGCCATTG GCACTATTAT TACCA [ 45] C.agasepsoides_194829_Bucan_Detwi ...... T. [ 45] C.agasepsoides_194830_Bucan_Detwi ...... T. [ 45] C.curtissi_aporus_102609_La_Descubierta ...... T....TT..T T...... A..... C.... [ 45] C.curtissi_aporus_102610_La_Descubierta ...... T....TT..T T...... A...... [ 45] C.curtissi_aporus_102611_La_Descubierta ...... T....TT..T T...... A..... C.... [ 45] C.curtissi_aporus_102706_Barahona ...... T....TT..T T...... A..... C.... [ 45] C.curtissi_aporus_102707_Juancho ...... T....TT..T T...... A...... [ 45] C.curtissi_aporus_102709_Juancho ...... T....TT..T T...... A...... [ 45] C.curtissi_aporus_102716_Barahona ...... T....TT..T T...... A..... C.... [ 45] C.curtissi_aporus_102915_Los_Arroyos ...... T....TT..T T...... A..... C.... [ 45] C.curtissi_aporus_102930_Barahona ...... T....TT..T T...... A..... C.T.. [ 45] C.curtissi_aporus_194831_Bucan_Detwi ...... T....TT..T T...... A..... C.... [ 45] C.curtissi_aporus_194832_Bucan_Detwi ...... T....TT..T T...... A..... C.... [ 45] C.curtissi_aporus_194833_Bucan_Detwi ...... T....TT..T T...... A..... C.... [ 45] C.curtissi_aporus_194838_Bucan_Detwi ...... T....TT..T T...... A..... C.... [ 45] C.badius_194964_Navassa_Island ...... T....T...T ...... CC. ....A..C...... [ 45] C.costatus_costatus_103085_Castillion .G...... T....TT..T ...... A..... C.... [ 45] C.costatus_costatus_104113_Castillion .G...... T....TT..T ...... A...... [ 45] C.costatus_costatus_192054_Abricots ..G...... T....TT..T ...... A..... C.... [ 45] C.costatus_costatus_192260_Beaumont .G...... T....TT..T ...... A..... C.... [ 45] C.cyanochloris_194930_Costa_Rica ....T..... CTCCTT...... C...... C..C.. C..A. [ 45]

C.curtissi_curtissi_194503_La_Descubierta ...... T....TT..T T...... A..C.. C.... [ 45] C.darlingtoni_161686_Cacique_Enriquillo ...... T....T.... T...... C.. .A..A..C.. C.... [ 45] C.darlingtoni_161687_Cacique_Enriquillo ...... T....T...T T...... C.. .A..A..C.. C.... [ 45] C.darlingtoni_161688_Cacique_Enriquillo ...... T....T.... T...... C.. .A..A..C.. C.... [ 45] C.curtissi_diastatus_194553_Mole_St._Nicolas ...... T....TT... T...... A..... C.... [ 45] C.curtissi_diastatus_194580_Mole_St._Nicolas ...... T....TT... T...... A..... C.... [ 45] C.enneagrammus_191044_Mexico ------[ 45] C.maculatus_266550_Cayman_Brac_West ...... T....TT...... A..C...... [ 45] C.maculatus_266551_Cayman_Brac_West ...... T....TT...... A..C...... [ 45] C.maculatus_266552_Cayman_Brac_West ...... T....TT...... A..C...... [ 45] C.maculatus_266553_Cayman_Brac_West ...... T....TT...... A..C...... [ 45] C.maculatus_266527_Cayman_Brac_West ...... T....TT...... A..C...... [ 45] C.marcanoi_193279_Valle_de_Bao ...... T....TT..T T...... A..... C.... [ 45] C.marcanoi_193280_Valle_de_Bao ...... T....TT..T T...... A..... C.... [ 45] C.marcanoi_193286_Valle_de_Bao ...... T....TT..T T...... A..... C.... [ 45] C.marcanoi_193309_Valle_de_Bao ...... T....TT..T T...... A..... C.... [ 45] C.marcanoi_193310_Valle_de_Bao ...... T....TT..T T...... A..... C.... [ 45] C.haetiana_mylicus_102546_Cabral ...... T....TT...... A...... [ 45] C.haetiana_mylicus_102547_Cabral ...... T....TT...... A...... [ 45] C.haetiana_mylicus_102550_Cabral ...... T....TT...... A...... [ 45] C.haetiana_mylicus_160201_Tejunde ...... T....TT...... A...... [ 45] C.costatus_neiba_102922_La_Descubierta ...... T....T...T T...... A..... C.... [ 45] C.costatus_neiba_102923_Los_Pinos ...... T....T...T T...... A..... C.... [ 45] C.costatus_neiba_102924_Los_Pinos ...... T....T...T T...... A..... C.... [ 45]

C.costatus_neiba_102925_Los_Pinos ...... T....T...T T...... A..... C.... [ 45] C.costatus_neiba_161498_Cacique_Enriquillo ...... T....T...T T.G...G...... A..... C.... [ 45] C._costatus_neiba_194362_Apolinar_Pelodroma ...... T....T...T T.G...G...... A..... C.... [ 45] C.costatus_neiba_194363_Apolinar_Pelodroma ...... T....T...T T.G...G...... A..... C.... [ 45] C.costatus_neiba_266321_Rosa_de_La_Pierda C...... T....T...T T.G...G...... A..... C.... [ 45] C.costatus_neiba_266683_Rosa_de_La_Pierda ...... T....T...T T.G...G...... A..... C.... [ 45] C.costatus_oreistes_104408_Jacmel C...... T....TT..T ...... A..C.. C.... [ 45] C.costatus_oreistes_192480_Puerto_Escondido C...... C....TT..T ...... A..C.. C.... [ 45] C.sepsoides_102369_Sabana_de_La_Mar .G...... T....TT.G. ..G....CC. ....A..C.. C.... [ 45] C.sepsoides_191850_Pestel ....T..... T....T...... C.C. ....A..C.. C...G [ 45] C.sepsoides_191913_Marche_Leon ....T..... T....T...... C.C. .T..A..C.. C...G [ 45] C.sepsoides_192469_La_Zanga .G...... T....TT.G. ..G....CC. ....A..C.. C.... [ 45] C.sepsoides_193201_Tenares .G...C.... T....TT.G. ..G....CC. ....A..C.. C.... [ 45] C.sepsoides_266748_Jacmel .G...C.... T....TT.G. ..G....CC. ....A..C.. C.... [ 45] C.sepsoides_266749_Jacmel .G...C.... T....TT.G. ..G....CC. ....A..C.. C.... [ 45] C.stenurus_stenurus_103900_Rampe_des_Lions_Boios_Sec ...... T....TT..T ...... A..C...... [ 45] C.stenurus_stenurus_103901_Rampe_des_Lions_Boios_Sec ...... T....TT..T ...... A..C...... [ 45] C.stenurus_stenurus_103902_Rampe_des_Lions_Boios_Sec ...... T....TT..T ...... A..C...... [ 45] C.stenurus_stenurus_267494_lle_a_Vache ...... T....TT..T ...... A..C...... [ 45] C.haetiana_surdus_104418_Gros_Cheval ...... T....TT...... A...... [ 45] C.haetiana_surdus_104419_Gros_Cheval ...... T....TT...... A...... [ 45] C.haetiana_surdus_192486_Puerto_Escondido ...... T....TT..T ...... T..A...... [ 45] C.haetiana_surdus_192497_Puerto_Escondido ...... T....TT..T ...... T..A...... [ 45] C.stenurus_rugosus_101247_Sabana_de_La_Mar ...... T....TT...... A..C...... [ 45]

C.stenurus_rugosus_101248_Sabana_de_La_Mar ...... T....TT...... A..C...... [ 45] C.stenurus_rugosus_102371_El_Valle ...... T....TT...... A..C...... [ 45] C.stenurus_rugosus_103075_Las_Galeras ...... T....TT...... A..C...... [ 45] C.stenurus_rugosus_103083_Nagua ...... T....TT...... A..C...... [ 45] C.stenurus_rugosus_103084_Nagua ...... T....TT...... A..C...... [ 45] C.stenurus_rugosus_193191_San_Francisco_de_Macoris ...... T....TT...... A..C...... [ 45] C.stenurus_rugosus_193192_San_Francisco_de_Macoris ...... T....TT...... A..C...... [ 45] C.stenurus_rugosus_193197_Tenares ...... T....TT...... A..C...... [ 45] C.stenurus_rugosus_193198_Tenares ...... T....TT...... A..C...... [ 45] C.stenurus_rugosus_193216_Tenares ...... T....TT...... A..C...... [ 45] C.stenurus_rugosus_193217_Tenares ...... T....TT...... A..C...... [ 45] C.warreni_172914_Hispaniola ...... T....T...T T...... C.. .TG.A..C.. C.... [ 45] C.warreni_194521_Puerto_Plata ...... T....T...T T...... C.. .T..A..C.. C.G.. [ 45] C.stenurus_weinlandi_102712_Canoa ...... T....T...... A..C...... [ 45] C.stenurus_weinlandi_103607_Soliette ...... T....TT...... A..C.. C.... [ 45] C.stenurus_weinlandi_191591_Thomaseau ...... T....TT...... A..C...... [ 45] C.stenurus_weinlandi_191622_Thomaseau ...... T....TT...... A..C...... [ 45] C.stenurus_weinlandi_192405_Thomaseau ...... T....TT...... A..C...... [ 45] C.stenurus_weinlandi_266286_El_Azul ...... T....T...... A...... [ 45] C.stenurus_weinlandi_266682_La_Zurza ...... T....T...... A..C.. C.... [ 45] C.stenurus_weinlandi_266684_La_Zurza ...... T....T...... A..C...... [ 45] C.stenurus_weinlandi_266685_La_Zurza ...... T....T...... A..C...... [ 45] D.bilobatus_194940_Costa_Rica CCT....C.. ...T.TA..T ..TATTT.G. ....C...... [ 45] D.nigropunctatus_191015_San_Luis_de_Potosi CC.....C.. ...CTT...... CA. .A.....C.. C.... [ 45]

D.nigropunctatus_191319_Los_Calderos CC.....C.. ...CTT...... CA. .A..C..C.. C.... [ 45] D.nigropunctatus_191320_Los_Calderos CC.....C.. ...CTT...... CA. .A..C..C.. C.... [ 45] D.nigropunctatus_191542_San_Vincente CC.....C.. TG.CTT...... CA. .A...... C AC... [ 45] D.garridoi_193507_El_Manguito CC.....C.. .G.CTT...... CA. .A...... C.... [ 45] D.pleii_191574_Rio_Abajo CC.....C.. ...CTTT...... CA. .A..C...... [ 45] C.barbouri_161120_Quick_Step ...... T....TT...... A...... [ 45] C.barbouri_190678_Tejunde ...... T....TT...... A..... C.... [ 45] C.crusculus_101571_Ewarton ...... T....TT...... A...... [ 45] C.crusculus_101572_Content ...... T....TT...... A...... [ 45] C.crusculus_172442_Duncans .G...... T....TT...... A..... C.... [ 45] C.crusculus_101554_Town_Head ...... T....TT...... A...... [ 45] C.crusculus_103448_Knoxwood ..C...... T....TT...... A..... C.... [ 45] C.crusculus_103449_Knowxwood ...... T....TT...... A..... C.... [ 45] C.crusculus_103460_Jackson_Bay ...... T....TT...... A..... C.... [ 45] C.crusculus_103586_Jackson_Bay .G...... T....TT...... A..... C.... [ 45] C.crusculus_161123_Duncans ....T..... T....TT...... A..... C.... [ 45] C.crusculus_161124_Salt_Gut .G...... T....TT...... A..... C.... [ 45] C.crusculus_171439_Oracabessa .G...... T....TT...... A..... C.... [ 45] C.crusculus_171601_Port_Maria .G...... T....TT...... A..... C.... [ 45] C.crusculus_171603_Port_Maria .G...... T....TT...... A..... C.... [ 45] C.crusculus_171604_Port_Maria .G...... T....TT...... A..... C.... [ 45] C.crusculus_171605_Port_Maria .G...... T....TT...... A..... C.... [ 45] C.crusculus_171606_Oracabessa .G...... T....TT...... A..... C.... [ 45] C.crusculus_171607_Braeton ...... T....TT...... A..... C.... [ 45]

C.crusculus_172438_Oracabessa .G...... T....TT...... A..... C.... [ 45] C.crusculus_172441_Old_Hope ...... T....TT...... A..... C.... [ 45] C.crusculus_172465_Section ..G....C.. T..G.TT... ..G.....C. ....A..C.. C.... [ 45] C.duquesneyi_267952_Hellshire_Hills ...... T....TT...... A..... C.... [ 45] C.hewardii_267097_Mandeville ...... T..G.TT...... A..C.. C.... [ 45] C.crusculus_101573_Hanover_Content .G...... T....TT...... A..... C.... [ 45] C.crusculus_101615_Oracabessa ...... T....TT...... A...... [ 45] C.crusculus_172439_Duncans .G...... T....TT...... A..... C.... [ 45] C.crusculus_172440_Old_Hope ...... T....TT...... A..... C.... [ 45] C.crusculus_190695_Duncans ...... T....TT...... A..... C.... [ 45] C.sepsoides_193116_Majagual .G...C.... T....TT.G. ..G....CC. ....A..C.. C.... [ 45] C.sepsoides_193117_Majagual .G...C.... T....TT.G. ..G....CC. ....A..C.. C.... [ 45] C.sepsoides_193118_Majagual .G...C.... T....TT.G. ..G....CC. ....A..C.. C.... [ 45] C.sepsoides_193202_Tenares .G...C.... T....TT.G. ..G....CC. ....A..C.. C.... [ 45] C.sepsoides_193203_Tenares .G...C.... T....TT.G. ..G....CC. ....A..C.. C.... [ 45] C.sepsoides_193204_Tenares .G...C.... T....TT.G. ..G....CC. ....A..C.. C.... [ 45] C.sepsoides_193040_Cotui .G...C.... T....TT.G. ..G....CC. ....A..C.. C.... [ 45] C.sepsoides_193041_Cotui .G...C.... T....TT.G. ..G....CC. ....A..C.. C.... [ 45] C.sepsoides_193042_Cotui .G...C.... T....TT.G. ..G....CC. ....A..C.. C.... [ 45] C.sepsoides_191851_Pestel .G...C.... T....TT.G. ..G....CC. ....A..C.. C.... [ 45] C.sepsoides_191914_Marche_Leon .G..T..... T....T...... C. ....A..C.. C.... [ 45] C.sepsoides_191915_Marche_Leon ...... C....TT...... C.C. ....A..C.. C...G [ 45] C.sepsoides_192313_Baraderes ....T..... T....TT...... C.C. .T..A..C.. C...G [ 45] D.pleii_191575_Rio_Abajo CC.....C.. ...CTTT...... CA. .A..C...... [ 45]

100

D.pleii_191576_Rio_Abajo CC.....C.. ...CTTT...... CA. .A..C...... [ 45] O.apodus_194938 .CT.A..CT. T..CCT...... T....CA. .A...... C.... [ 45] C.agasepsoides_160191_Canoa TATCAAGCCA TCACTGGCTT CTAGCATGAA TAGGTTTAGA ACTCA [ 90] C.agasepsoides_194829_Bucan_Detwi ...... C ...... [ 90] C.agasepsoides_194830_Bucan_Detwi ...... C ...... [ 90] C.curtissi_aporus_102609_La_Descubierta ...... C.....A..A ...... C...... A. [ 90] C.curtissi_aporus_102610_La_Descubierta ...... C.....A..A ...... CC...... A. [ 90] C.curtissi_aporus_102611_La_Descubierta ...... C.....A..A ...... C...... A. [ 90] C.curtissi_aporus_102706_Barahona ...... C.....A..A ...... C...... A. [ 90] C.curtissi_aporus_102707_Juancho ...... C.....A..A ...... C...... A. [ 90] C.curtissi_aporus_102709_Juancho ...... C.....A..A ...... C...... A. [ 90] C.curtissi_aporus_102716_Barahona ...... C.....A..A ...... C...... A. [ 90] C.curtissi_aporus_102915_Los_Arroyos ....G..... C.....A..A ...... C...... A. [ 90] C.curtissi_aporus_102930_Barahona ...... C.....A..A ...... C...... A. [ 90] C.curtissi_aporus_194831_Bucan_Detwi ...... C.....A..A ...... C...... A. [ 90] C.curtissi_aporus_194832_Bucan_Detwi ...... C.....A..A ...... C...... A. [ 90] C.curtissi_aporus_194833_Bucan_Detwi ...... C.....A..A ...... C...... A. [ 90] C.curtissi_aporus_194838_Bucan_Detwi ...... C.....A..A ...... C...... A. [ 90] C.badius_194964_Navassa_Island ...... C.....A..A ...... CC...... G. [ 90] C.costatus_costatus_103085_Castillion ...... C.....A..A ..G...... C...... T. [ 90] C.costatus_costatus_104113_Castillion ...... C.....A..A ..G...... C...... T. [ 90] C.costatus_costatus_192054_Abricots ...... C.....A..A ...... C...... T. [ 90] C.costatus_costatus_192260_Beaumont ...... C.....A..A ..G...... C...... T. [ 90] C.cyanochloris_194930_Costa_Rica ....T...A...... A..A ...... CC...... A. [ 90]

101

C.curtissi_curtissi_194503_La_Descubierta ...... C.....A..A ...... C...... A. [ 90] C.darlingtoni_161686_Cacique_Enriquillo ...... C.....A..A T.G...... CC.G.. ...T. [ 90] C.darlingtoni_161687_Cacique_Enriquillo ...... C.....A..A T.G...... CC.G.. ...T. [ 90] C.darlingtoni_161688_Cacique_Enriquillo ...... C.....A..A T.G...... CC.G.. ...T. [ 90] C.curtissi_diastatus_194553_Mole_St._Nicolas ...... C.....A..A ...... C...... A. [ 90] C.curtissi_diastatus_194580_Mole_St._Nicolas ...... C.....A..A ...... C...... A. [ 90] C.enneagrammus_191044_Mexico ------. ...A. [ 90] C.maculatus_266550_Cayman_Brac_West ...... C.....A..A ...... C...... T. [ 90] C.maculatus_266551_Cayman_Brac_West ...... C.....A..A ...... GC...... T. [ 90] C.maculatus_266552_Cayman_Brac_West ...... C.....A..A ...... GC...... T. [ 90] C.maculatus_266553_Cayman_Brac_West ...... C.....A..A ...... GC...... T. [ 90] C.maculatus_266527_Cayman_Brac_West ...... C.....A..A ...... GC...... T. [ 90] C.marcanoi_193279_Valle_de_Bao ...... C.....A..A ...... C...... A. [ 90] C.marcanoi_193280_Valle_de_Bao ...... C.....A..A ...... C...... A. [ 90] C.marcanoi_193286_Valle_de_Bao ...... C.....A..A ...... C...... A. [ 90] C.marcanoi_193309_Valle_de_Bao ...... C.....A..A ...... C...... A. [ 90] C.marcanoi_193310_Valle_de_Bao ...... C.....A..A ...... C...... A. [ 90] C.haetiana_mylicus_102546_Cabral ...... T.. C.....A..A ..C...... C...... TAT. [ 90] C.haetiana_mylicus_102547_Cabral ...... T.. C.....A..A ..C...... C...... T. [ 90] C.haetiana_mylicus_102550_Cabral ...... T.. C.....A..A ..C...... C...... T. [ 90] C.haetiana_mylicus_160201_Tejunde ...... T.. C.....A..A ..C...... C...... T. [ 90] C.costatus_neiba_102922_La_Descubierta ...... C..T.....A ...... AC...... T. [ 90] C.costatus_neiba_102923_Los_Pinos ...... C.....A..A ...... AC...... A. [ 90] C.costatus_neiba_102924_Los_Pinos ...... C.....A..A ...... AC...... A. [ 90]

102

C.costatus_neiba_102925_Los_Pinos ...... C.....A..A ...... AC...... A. [ 90] C.costatus_neiba_161498_Cacique_Enriquillo ...... A..A ...... C...... A. [ 90] C._costatus_neiba_194362_Apolinar_Pelodroma ...... A..A ...... C...... A. [ 90] C.costatus_neiba_194363_Apolinar_Pelodroma ...... A..A ...... C...... A. [ 90] C.costatus_neiba_266321_Rosa_de_La_Pierda ...... A..A ...... C...... A. [ 90] C.costatus_neiba_266683_Rosa_de_La_Pierda ...... A..A ...... C...... A. [ 90] C.costatus_oreistes_104408_Jacmel ...... A..A ...... C...... T. [ 90] C.costatus_oreistes_192480_Puerto_Escondido ...... C.....A..A ...... C...... T. [ 90] C.sepsoides_102369_Sabana_de_La_Mar ...... C...... A ...... C...... A. [ 90] C.sepsoides_191850_Pestel C...... C.....A..A ...... T. [ 90] C.sepsoides_191913_Marche_Leon C...... C.....A..A .....G...... T. [ 90] C.sepsoides_192469_La_Zanga ...... C...... A ...... C...... A. [ 90] C.sepsoides_193201_Tenares ...... C...... T.G ...... C...... A. [ 90] C.sepsoides_266748_Jacmel ...... C...... T.G ...... C...... A. [ 90] C.sepsoides_266749_Jacmel ...... C...... T.G ...... C...... A. [ 90] C.stenurus_stenurus_103900_Rampe_des_Lions_Boios_Sec ...... C.....A..A ...... G..CC...... T. [ 90] C.stenurus_stenurus_103901_Rampe_des_Lions_Boios_Sec ...... C.....A..A ...... G..CC...... T. [ 90] C.stenurus_stenurus_103902_Rampe_des_Lions_Boios_Sec ...... C.....A..A ...... G..CC...... T. [ 90] C.stenurus_stenurus_267494_lle_a_Vache ...... C.....A..A ...... G..CC...... T. [ 90] C.haetiana_surdus_104418_Gros_Cheval ...... T.. C...... A ...... C...... T. [ 90] C.haetiana_surdus_104419_Gros_Cheval ...... T.. C...... A ...... C...... T. [ 90] C.haetiana_surdus_192486_Puerto_Escondido ...... T.. C.....A..A ...... T. [ 90] C.haetiana_surdus_192497_Puerto_Escondido ...... T.. C.....A..A ...... T. [ 90] C.stenurus_rugosus_101247_Sabana_de_La_Mar ...... C.....A..A ...... G..AC...... T. [ 90]

103

C.stenurus_rugosus_101248_Sabana_de_La_Mar ...... C.....A..A ...... G..AC...... T. [ 90] C.stenurus_rugosus_102371_El_Valle ...... C.....A..A ...... G..AC...... T. [ 90] C.stenurus_rugosus_103075_Las_Galeras ...... C.....A..A ...... G..AC...... T. [ 90] C.stenurus_rugosus_103083_Nagua ...... C.....A..A ...... G..AC...... T. [ 90] C.stenurus_rugosus_103084_Nagua ...... C.....A..A ...... G..AC...... T. [ 90] C.stenurus_rugosus_193191_San_Francisco_de_Macoris ...... C.....A..A ...... G..AC...... T. [ 90] C.stenurus_rugosus_193192_San_Francisco_de_Macoris ...... C.....A..A ...... G..AC...... T. [ 90] C.stenurus_rugosus_193197_Tenares ...... C.....A..A ...... G..AC...... T. [ 90] C.stenurus_rugosus_193198_Tenares ...... C.....A..A ...... G..AC...... T. [ 90] C.stenurus_rugosus_193216_Tenares ...... C.....A..A ...... G..AC...... T. [ 90] C.stenurus_rugosus_193217_Tenares ...... C.....A..A ...... G..AC...... T. [ 90] C.warreni_172914_Hispaniola ...... C.....A...... CC...... T. [ 90] C.warreni_194521_Puerto_Plata ...... C.....A...... CC...... T. [ 90] C.stenurus_weinlandi_102712_Canoa ...... C.....A..A ...... G..AC...... T. [ 90] C.stenurus_weinlandi_103607_Soliette ...... C.....A..A ...... G..AC...... T. [ 90] C.stenurus_weinlandi_191591_Thomaseau ...... C.....A..A ...... AC...... T. [ 90] C.stenurus_weinlandi_191622_Thomaseau ...... C.....A..A ...... AC...... T. [ 90] C.stenurus_weinlandi_192405_Thomaseau ...... C.....A..A ...... G..AC...... T. [ 90] C.stenurus_weinlandi_266286_El_Azul ...... C.....A..A ...... G..AC...... T. [ 90] C.stenurus_weinlandi_266682_La_Zurza ...... C.....A..A ...... G..AC...... T. [ 90] C.stenurus_weinlandi_266684_La_Zurza ...... C.....A..A ...... G..AC...... T. [ 90] C.stenurus_weinlandi_266685_La_Zurza ...... C.....A..A ...... G..AC...... T. [ 90] D.bilobatus_194940_Costa_Rica ...... T.. C.....A..G .....C.... .G..AC.... .T.A. [ 90] D.nigropunctatus_191015_San_Luis_de_Potosi ....C..... C.....A... A.C...... C ....AC...... A. [ 90]

104

D.nigropunctatus_191319_Los_Calderos ....C..... C.....A... A.C...... C ....AC...... A. [ 90] D.nigropunctatus_191320_Los_Calderos ....C..... C.....A... A.C...... C ....AC...... A. [ 90] D.nigropunctatus_191542_San_Vincente ....C..... C.....A... A.C...... C ....GC...... A. [ 90] D.garridoi_193507_El_Manguito .G..C..... C.....A... A.C...... C ....AC...... A. [ 90] D.pleii_191574_Rio_Abajo ..A.C..T.. C.....A..A A.T...... C ....AC...... A. [ 90] C.barbouri_161120_Quick_Step ....G..... C.....A..A ...... G....A... C..T. [ 90] C.barbouri_190678_Tejunde ....G..... C.....A..A ...... G...... T. [ 90] C.crusculus_101571_Ewarton ...... C.....A..A ...... G..CC...... T. [ 90] C.crusculus_101572_Content ...... C.....A..A ...... G..CCC...... T. [ 90] C.crusculus_172442_Duncans ...... C.....A..A ...... G...C...... A. [ 90] C.crusculus_101554_Town_Head ...... C.....A..A ...... G..CC...... T. [ 90] C.crusculus_103448_Knoxwood ...... T.. C.....A..A ...... G...... A. [ 90] C.crusculus_103449_Knowxwood ...... T.. C.....A..A ...... G...... A. [ 90] C.crusculus_103460_Jackson_Bay ...... C.....A..A ...... G...C...... A. [ 90] C.crusculus_103586_Jackson_Bay ...... C.....A..A ...... G...C...... A. [ 90] C.crusculus_161123_Duncans ...... C.....A..A ...... G..C...... T. [ 90] C.crusculus_161124_Salt_Gut ...... C.....A..A ...... G...C...... A. [ 90] C.crusculus_171439_Oracabessa ...... C.....A..A ...... G...C...... A. [ 90] C.crusculus_171601_Port_Maria ...... C.....A..A ...... G...C...... A. [ 90] C.crusculus_171603_Port_Maria ...... C.....A..A ...... G...C...... A. [ 90] C.crusculus_171604_Port_Maria ...... C.....A..A ...... G...C...... A. [ 90] C.crusculus_171605_Port_Maria ...... C.....A..A ...... G...C...... A. [ 90] C.crusculus_171606_Oracabessa ...... C.....A..A ...... G...C...... A. [ 90] C.crusculus_171607_Braeton ...... C.....A..A ...... G..C...... T. [ 90]

105

C.crusculus_172438_Oracabessa ...... C.....A..A ...... G...C...... A. [ 90] C.crusculus_172441_Old_Hope ...... C.....A..A ...... T. [ 90] C.crusculus_172465_Section ...... C.....A..A ...... G..C..... G..T. [ 90] C.duquesneyi_267952_Hellshire_Hills ...... C.....A..A ...... G..CC...... T. [ 90] C.hewardii_267097_Mandeville ...... C.....A..A ...... G..C...... CT. [ 90] C.crusculus_101573_Hanover_Content ...... C.....A..A ...... G...C...... A. [ 90] C.crusculus_101615_Oracabessa ...... C.....A..A ...... G..CC...... T. [ 90] C.crusculus_172439_Duncans ...... C.....A..A ...... G...C...... A. [ 90] C.crusculus_172440_Old_Hope ...... C.....A..A ...... G..C...... T. [ 90] C.crusculus_190695_Duncans ...... T.. C.....A..A ...... G...... A. [ 90] C.sepsoides_193116_Majagual ...... C...... T.G ...... C...... A. [ 90] C.sepsoides_193117_Majagual ...... C...... T.G ...... C...... A. [ 90] C.sepsoides_193118_Majagual ...... C...... T.G ...... C...... A. [ 90] C.sepsoides_193202_Tenares ...... C...... T.G ...... C...... A. [ 90] C.sepsoides_193203_Tenares ...... C...... T.G ...... C...... A. [ 90] C.sepsoides_193204_Tenares ...... C...... T.G ...... C...... A. [ 90] C.sepsoides_193040_Cotui ...... C...... T.G ...... C...... A. [ 90] C.sepsoides_193041_Cotui ...... C...... T.G ...... C...... A. [ 90] C.sepsoides_193042_Cotui ...... C...... T.G ...... C...... A. [ 90] C.sepsoides_191851_Pestel ...... C...... T.G ...... C...... A. [ 90] C.sepsoides_191914_Marche_Leon ...... C.....A..A ...... G..C..... G..T. [ 90] C.sepsoides_191915_Marche_Leon C...... C.....A..A .....G...... C...... T. [ 90] C.sepsoides_192313_Baraderes C...... C.....A..A .....G...... T. [ 90] D.pleii_191575_Rio_Abajo ..A.C..T.. C.....A..A A.T...... C ....AC...... A. [ 90]

106

D.pleii_191576_Rio_Abajo ..A.C..T.. C.....A..A A.T...... C ....AC...... A. [ 90] O.apodus_194938 ..G.C...TT C...... C ..T...... AC...... A. [ 90] C.agasepsoides_160191_Canoa ACACCCTTGC CATCCTGCCA GTCATTTCAA AAATCCACCA CCCCC [135] C.agasepsoides_194829_Bucan_Detwi ...... A...... [135] C.agasepsoides_194830_Bucan_Detwi ...... A...... [135] C.curtissi_aporus_102609_La_Descubierta .T...... A... A....C...... [135] C.curtissi_aporus_102610_La_Descubierta .T...... A... A....C...... [135] C.curtissi_aporus_102611_La_Descubierta .T...... A... A....C...... [135] C.curtissi_aporus_102706_Barahona .T...... A... A....C...... [135] C.curtissi_aporus_102707_Juancho .T...... A... A....C...... [135] C.curtissi_aporus_102709_Juancho .T...... A... A....C...... [135] C.curtissi_aporus_102716_Barahona .T...... A... A....C...... [135] C.curtissi_aporus_102915_Los_Arroyos .T...... A... A....C...... [135] C.curtissi_aporus_102930_Barahona .T.....G...... A... A....C...... [135] C.curtissi_aporus_194831_Bucan_Detwi .T...... A... A....C...... [135] C.curtissi_aporus_194832_Bucan_Detwi .T...... A... A....C...... [135] C.curtissi_aporus_194833_Bucan_Detwi .T...... A... A....C...... [135] C.curtissi_aporus_194838_Bucan_Detwi .T...... A... A....C...... [135] C.badius_194964_Navassa_Island .T...... A... A....C...... [135] C.costatus_costatus_103085_Castillion .T...... A... A....C...... A. [135] C.costatus_costatus_104113_Castillion .T...... A... A....C...... A. [135] C.costatus_costatus_192054_Abricots .T...... A... A....C...... A. [135] C.costatus_costatus_192260_Beaumont .T...... A... A....C...... A. [135] C.cyanochloris_194930_Costa_Rica ....A..A.. A.....C..C A.T..C...... TCA...... A. [135]

107

C.curtissi_curtissi_194503_La_Descubierta .T...... A... A....C...... A. [135] C.darlingtoni_161686_Cacique_Enriquillo .TG....A.. T...... A.T..C...... G. [135] C.darlingtoni_161687_Cacique_Enriquillo .T.....A.. T...... A.T..C...... G. [135] C.darlingtoni_161688_Cacique_Enriquillo .TG....A.. T...... A.T..C...... G. [135] C.curtissi_diastatus_194553_Mole_St._Nicolas ...... A... A....C...... A. [135] C.curtissi_diastatus_194580_Mole_St._Nicolas ...... A... A....C...... A. [135] C.enneagrammus_191044_Mexico ....AT.A...... C... A.T..C.... .GTCT..T.. ...A. [135] C.maculatus_266550_Cayman_Brac_West ...... C...... A. [135] C.maculatus_266551_Cayman_Brac_West ...... C...... A. [135] C.maculatus_266552_Cayman_Brac_West ...... C...... A. [135] C.maculatus_266553_Cayman_Brac_West ...... C...... A. [135] C.maculatus_266527_Cayman_Brac_West ...... C...... A. [135] C.marcanoi_193279_Valle_de_Bao .T...... A... A....C...... A. [135] C.marcanoi_193280_Valle_de_Bao .T...... A... A....C...... A. [135] C.marcanoi_193286_Valle_de_Bao .T...... A... A....C...... A. [135] C.marcanoi_193309_Valle_de_Bao .T...... A... A....C...... A. [135] C.marcanoi_193310_Valle_de_Bao .T...... A... A....C...... A. [135] C.haetiana_mylicus_102546_Cabral .T...... A... A.T..C...... T.... [135] C.haetiana_mylicus_102547_Cabral .T...... A... A.T..C...... T.... [135] C.haetiana_mylicus_102550_Cabral .T...... A... A.T..C...... T.... [135] C.haetiana_mylicus_160201_Tejunde .T...... A... A.T..C...... T.... [135] C.costatus_neiba_102922_La_Descubierta ...... A... A....C...... T...... A. [135] C.costatus_neiba_102923_Los_Pinos ...... A... A.T..C...... T...... A. [135] C.costatus_neiba_102924_Los_Pinos ...... A... A....C...... T...... A. [135]

108

C.costatus_neiba_102925_Los_Pinos ...... A... A.T..C...... T...... A. [135] C.costatus_neiba_161498_Cacique_Enriquillo .T...... A... A....C...... T...... A. [135] C._costatus_neiba_194362_Apolinar_Pelodroma .T...... A... A....C...... T...... A. [135] C.costatus_neiba_194363_Apolinar_Pelodroma .T...... A... A....C...... T...... A. [135] C.costatus_neiba_266321_Rosa_de_La_Pierda .T...... A... A....C...... T...... A. [135] C.costatus_neiba_266683_Rosa_de_La_Pierda .T...... A... A....C...... T...... A. [135] C.costatus_oreistes_104408_Jacmel .T...... A....C...... A. [135] C.costatus_oreistes_192480_Puerto_Escondido .T...... A....C...... A. [135] C.sepsoides_102369_Sabana_de_La_Mar .T...... A... A....C...... A. [135] C.sepsoides_191850_Pestel .T...... T.A... A....C...... A. [135] C.sepsoides_191913_Marche_Leon .T...... T.A... A....C...... A. [135] C.sepsoides_192469_La_Zanga .T...... T.A... A....C...... A. [135] C.sepsoides_193201_Tenares .T...... T.A... A....C...... A. [135] C.sepsoides_266748_Jacmel .T...... T.A... A....C...... A. [135] C.sepsoides_266749_Jacmel .T...... T.A... A....C...... A. [135] C.stenurus_stenurus_103900_Rampe_des_Lions_Boios_Sec ...... C...... A. [135] C.stenurus_stenurus_103901_Rampe_des_Lions_Boios_Sec ...... C...... A. [135] C.stenurus_stenurus_103902_Rampe_des_Lions_Boios_Sec ...... A...... C...... A. [135] C.stenurus_stenurus_267494_lle_a_Vache ...... A...... C...... A. [135] C.haetiana_surdus_104418_Gros_Cheval ...... A... A.T..C...... T.... [135] C.haetiana_surdus_104419_Gros_Cheval ...... A... A.T..C...... T.... [135] C.haetiana_surdus_192486_Puerto_Escondido ...... A... A.T..C...... T.... [135] C.haetiana_surdus_192497_Puerto_Escondido ...... A... A.T..C...... T.... [135] C.stenurus_rugosus_101247_Sabana_de_La_Mar .T...... C...... A. [135]

109

C.stenurus_rugosus_101248_Sabana_de_La_Mar .T...... C...... A. [135] C.stenurus_rugosus_102371_El_Valle .T...... C...... A. [135] C.stenurus_rugosus_103075_Las_Galeras .T...... C...... A. [135] C.stenurus_rugosus_103083_Nagua .T...... C...... A. [135] C.stenurus_rugosus_103084_Nagua .T...... C...... A. [135] C.stenurus_rugosus_193191_San_Francisco_de_Macoris .T...... C...... A. [135] C.stenurus_rugosus_193192_San_Francisco_de_Macoris .T...... C...... A. [135] C.stenurus_rugosus_193197_Tenares .T...... C...... A. [135] C.stenurus_rugosus_193198_Tenares .T...... C...... A. [135] C.stenurus_rugosus_193216_Tenares .T...... C...... A. [135] C.stenurus_rugosus_193217_Tenares .T...... C...... A. [135] C.warreni_172914_Hispaniola .T...T.A...... T.A... A.T..C...... A. [135] C.warreni_194521_Puerto_Plata .T...T.A...... T.A... A.T..C...... A. [135] C.stenurus_weinlandi_102712_Canoa .T...... C...... A. [135] C.stenurus_weinlandi_103607_Soliette .T...... C...... A. [135] C.stenurus_weinlandi_191591_Thomaseau .T...... C...... A. [135] C.stenurus_weinlandi_191622_Thomaseau .T...... C...... A. [135] C.stenurus_weinlandi_192405_Thomaseau .T...... C...... A. [135] C.stenurus_weinlandi_266286_El_Azul .T...... C...... A. [135] C.stenurus_weinlandi_266682_La_Zurza .T...... C...... A. [135] C.stenurus_weinlandi_266684_La_Zurza .T...... C...... A. [135] C.stenurus_weinlandi_266685_La_Zurza .T...... C...... A. [135] D.bilobatus_194940_Costa_Rica ....A..A.. A.....A..T A....C..C. ..C.T..T.. T..A. [135] D.nigropunctatus_191015_San_Luis_de_Potosi .....A.A...... A..C A.T..C...... TCA...... A. [135]

110

D.nigropunctatus_191319_Los_Calderos .....A.A...... A..C A.T..C...... TCA...... A. [135] D.nigropunctatus_191320_Los_Calderos .....A.A...... A..C A.T..C...... TCA...... A. [135] D.nigropunctatus_191542_San_Vincente .....A.A...... A..C A.T..C...... TCA...... A. [135] D.garridoi_193507_El_Manguito .....A.A...... A..C A.T..C...... TCA...... A. [135] D.pleii_191574_Rio_Abajo ...... G.. T.....A..C A.T..C..T. ...CA...... A. [135] C.barbouri_161120_Quick_Step .T...... A...... C...... A. [135] C.barbouri_190678_Tejunde .T...... A...... C...... A. [135] C.crusculus_101571_Ewarton .T...... A... A...... A. [135] C.crusculus_101572_Content .T...... A... A...... A. [135] C.crusculus_172442_Duncans .T...... A... A.T..C...... A. [135] C.crusculus_101554_Town_Head .T...... A... A....C...... A. [135] C.crusculus_103448_Knoxwood .T...... A... A....C...... A. [135] C.crusculus_103449_Knowxwood .T...... A... A....C...... A. [135] C.crusculus_103460_Jackson_Bay .T...... A... A....C...... A. [135] C.crusculus_103586_Jackson_Bay .T...... A... A....C...... A. [135] C.crusculus_161123_Duncans .T...... C... A....C...... [135] C.crusculus_161124_Salt_Gut .T...... A... A.T..C...... A. [135] C.crusculus_171439_Oracabessa .T...... A... A.T..C...... A. [135] C.crusculus_171601_Port_Maria .T...... A... A.T..C...... A. [135] C.crusculus_171603_Port_Maria .T...... A... A.T..C...... A. [135] C.crusculus_171604_Port_Maria .T...... A... A.T..C...... A. [135] C.crusculus_171605_Port_Maria .T...... A... A.T..C...... A. [135] C.crusculus_171606_Oracabessa .T...... A... A.T..C...... A. [135] C.crusculus_171607_Braeton .T...... A....C...... [135]

111

C.crusculus_172438_Oracabessa .T...... A... A.T..C...... A. [135] C.crusculus_172441_Old_Hope ...... A... A....C...... A. [135] C.crusculus_172465_Section .T...... T.....A... A....C...... A. [135] C.duquesneyi_267952_Hellshire_Hills .T...... A... A....C...... T.. ...A. [135] C.hewardii_267097_Mandeville ...... A... A...... A. [135] C.crusculus_101573_Hanover_Content .T...... A... A....C...... A. [135] C.crusculus_101615_Oracabessa .T...... A... A...... A. [135] C.crusculus_172439_Duncans .T...... A... A.T..C...... A. [135] C.crusculus_172440_Old_Hope .T...... C... A....C...... [135] C.crusculus_190695_Duncans .T...... A... A....C...... A. [135] C.sepsoides_193116_Majagual .T...... T.A... A....C...... A. [135] C.sepsoides_193117_Majagual .T...... T.A... A....C...... A. [135] C.sepsoides_193118_Majagual .T...... T.A... A....C...... A. [135] C.sepsoides_193202_Tenares .T...... T.A... A....C...... A. [135] C.sepsoides_193203_Tenares .T...... T.A... A....C...... A. [135] C.sepsoides_193204_Tenares .T...... T.A... A....C...... A. [135] C.sepsoides_193040_Cotui .T...... T.A... A....C...... A. [135] C.sepsoides_193041_Cotui .T...... T.A... A....C...... A. [135] C.sepsoides_193042_Cotui .T...... T.A... A....C...... A. [135] C.sepsoides_191851_Pestel .T...... T.A... A....C...... A. [135] C.sepsoides_191914_Marche_Leon ...... A...... T.A... A....C...... A. [135] C.sepsoides_191915_Marche_Leon .T...... A... A.T..C...... A. [135] C.sepsoides_192313_Baraderes .T...... T.A... A....C...... A. [135] D.pleii_191575_Rio_Abajo ...... G.. T.....A..C A.T..C..T. ...CA...... A. [135]

112

D.pleii_191576_Rio_Abajo ...... G.. T.....A..C A.T..C..T. ...CA...... A. [135] O.apodus_194938 ...... A.....A..C A....C..T. ..TCT...... A. [135] C.agasepsoides_160191_Canoa GAGCCACAGA AGCCTCCACC AAATACTTTC TTACACAAGC AGCGG [180] C.agasepsoides_194829_Bucan_Detwi ...... A. [180] C.agasepsoides_194830_Bucan_Detwi ...... A. [180] C.curtissi_aporus_102609_La_Descubierta .G...... A...... T .A...... A. [180] C.curtissi_aporus_102610_La_Descubierta .G...... A...... T .A...... A. [180] C.curtissi_aporus_102611_La_Descubierta .G...... A...... T .A...... A. [180] C.curtissi_aporus_102706_Barahona ...... A...... T .A...... A. [180] C.curtissi_aporus_102707_Juancho .G...... A...... T .A...... A. [180] C.curtissi_aporus_102709_Juancho .G...... A...... T .A...... A. [180] C.curtissi_aporus_102716_Barahona ...... A...... T .A...... A. [180] C.curtissi_aporus_102915_Los_Arroyos .G...... A...... T .A...... A. [180] C.curtissi_aporus_102930_Barahona ...... A...... T .A...... A. [180] C.curtissi_aporus_194831_Bucan_Detwi .G...... A...... T .A...... A. [180] C.curtissi_aporus_194832_Bucan_Detwi ...... A...... T .A...... A. [180] C.curtissi_aporus_194833_Bucan_Detwi ...... A...... T .A...... A. [180] C.curtissi_aporus_194838_Bucan_Detwi .G...... A...... T .A...... A. [180] C.badius_194964_Navassa_Island .G..T...... G...... A. [180] C.costatus_costatus_103085_Castillion .G...... A..T ...... T .A...... A. [180] C.costatus_costatus_104113_Castillion .G...... A..T ...... T .A...... A. [180] C.costatus_costatus_192054_Abricots .G...... A..T ...... T .A...... A. [180] C.costatus_costatus_192260_Beaumont .G...... A..T ...... T .A...... A. [180] C.cyanochloris_194930_Costa_Rica ...... T...... C. .A..G...... TA. [180]

113

C.curtissi_curtissi_194503_La_Descubierta .G...... A...... T .A...... A. [180] C.darlingtoni_161686_Cacique_Enriquillo ...... A...... C...... A. [180] C.darlingtoni_161687_Cacique_Enriquillo ...... A...... C...... A. [180] C.darlingtoni_161688_Cacique_Enriquillo ...... A...... C...... A. [180] C.curtissi_diastatus_194553_Mole_St._Nicolas .G...... A...... T .A...... A. [180] C.curtissi_diastatus_194580_Mole_St._Nicolas .G...... A...... T .A...... A. [180] C.enneagrammus_191044_Mexico ...... T..T ...... T .A...... C..A. [180] C.maculatus_266550_Cayman_Brac_West .G..T...... G...... A. [180] C.maculatus_266551_Cayman_Brac_West .G..T...... G...... A. [180] C.maculatus_266552_Cayman_Brac_West .G..T...... G...... A. [180] C.maculatus_266553_Cayman_Brac_West .G..T...... G...... A. [180] C.maculatus_266527_Cayman_Brac_West .G..T...... G...... A. [180] C.marcanoi_193279_Valle_de_Bao .G...... A...... A...... A. [180] C.marcanoi_193280_Valle_de_Bao .G...... A...... T .A...... A. [180] C.marcanoi_193286_Valle_de_Bao .G...... A...... T .A...... A. [180] C.marcanoi_193309_Valle_de_Bao .G...... A...... T .A...... A. [180] C.marcanoi_193310_Valle_de_Bao .G...... A...... T .A...... A. [180] C.haetiana_mylicus_102546_Cabral ...... C. .C...... A. [180] C.haetiana_mylicus_102547_Cabral ...... C. .C...... A. [180] C.haetiana_mylicus_102550_Cabral ...... C. .C...... A. [180] C.haetiana_mylicus_160201_Tejunde ...... C. .C...... A. [180] C.costatus_neiba_102922_La_Descubierta .G...... A...... T .A...... A. [180] C.costatus_neiba_102923_Los_Pinos .G...... A...... T .A...... A. [180] C.costatus_neiba_102924_Los_Pinos .G...... A...... T .A...... A. [180]

114

C.costatus_neiba_102925_Los_Pinos .G...... A...... T .A...... A. [180] C.costatus_neiba_161498_Cacique_Enriquillo .G...... A...... T .A...... A. [180] C._costatus_neiba_194362_Apolinar_Pelodroma .G...... A...... T .A...... A. [180] C.costatus_neiba_194363_Apolinar_Pelodroma .G...... A...... T .A...... A. [180] C.costatus_neiba_266321_Rosa_de_La_Pierda .G...... A...... T .A...... A. [180] C.costatus_neiba_266683_Rosa_de_La_Pierda .G...... A...... T .A...... A. [180] C.costatus_oreistes_104408_Jacmel .G...... A...... CT .A...... A. [180] C.costatus_oreistes_192480_Puerto_Escondido .G...... A...... CT .A...... A. [180] C.sepsoides_102369_Sabana_de_La_Mar ...... T .....T.... .C...... GA.A. [180] C.sepsoides_191850_Pestel ...... G...... C...... A.A. [180] C.sepsoides_191913_Marche_Leon ...... G...... T.... .C...... A.A. [180] C.sepsoides_192469_La_Zanga ...... T .....T...... GA.A. [180] C.sepsoides_193201_Tenares ...... T .....T...... GA.A. [180] C.sepsoides_266748_Jacmel ...... T .....T...... GA.A. [180] C.sepsoides_266749_Jacmel ...... T .....T...... GA.A. [180] C.stenurus_stenurus_103900_Rampe_des_Lions_Boios_Sec .G..T...... G...... A. [180] C.stenurus_stenurus_103901_Rampe_des_Lions_Boios_Sec .G..T...... G...... A. [180] C.stenurus_stenurus_103902_Rampe_des_Lions_Boios_Sec .G..T...... G...... A. [180] C.stenurus_stenurus_267494_lle_a_Vache .G..T...... G...... A. [180] C.haetiana_surdus_104418_Gros_Cheval ...... T.... .C...... A. [180] C.haetiana_surdus_104419_Gros_Cheval ...... T.... .C...... A. [180] C.haetiana_surdus_192486_Puerto_Escondido ...... C...... A. [180] C.haetiana_surdus_192497_Puerto_Escondido ...... C...... A. [180] C.stenurus_rugosus_101247_Sabana_de_La_Mar .G..T...... G...... C...... A. [180]

115

C.stenurus_rugosus_101248_Sabana_de_La_Mar .G..T...... G...... C...... A. [180] C.stenurus_rugosus_102371_El_Valle .G..T...... G...... C...... A. [180] C.stenurus_rugosus_103075_Las_Galeras .G..T...... G...... C...... [180] C.stenurus_rugosus_103083_Nagua .G..T...... G...... C...... A. [180] C.stenurus_rugosus_103084_Nagua .G..T...... G...... C...... A. [180] C.stenurus_rugosus_193191_San_Francisco_de_Macoris .G..T...... G...... C...... A. [180] C.stenurus_rugosus_193192_San_Francisco_de_Macoris .G..T...... G...... C...... A. [180] C.stenurus_rugosus_193197_Tenares .G..T...... G...... C...... A. [180] C.stenurus_rugosus_193198_Tenares .G..T...... G...... C...... A. [180] C.stenurus_rugosus_193216_Tenares .G..T...... G...... C...... A. [180] C.stenurus_rugosus_193217_Tenares .G..T...... G...... C...... A. [180] C.warreni_172914_Hispaniola ...... A...... T...... A. [180] C.warreni_194521_Puerto_Plata ...... A...... T...... A. [180] C.stenurus_weinlandi_102712_Canoa .G..T...... C...... A. [180] C.stenurus_weinlandi_103607_Soliette .G..T..... G...... G...... C...... A. [180] C.stenurus_weinlandi_191591_Thomaseau .G..T..... G...... C...... A. [180] C.stenurus_weinlandi_191622_Thomaseau .G..T..... G...... C...... A. [180] C.stenurus_weinlandi_192405_Thomaseau .G..T..... G...... G...... C...... A. [180] C.stenurus_weinlandi_266286_El_Azul .G..T...... C...... A. [180] C.stenurus_weinlandi_266682_La_Zurza .G..T...... C...... A. [180] C.stenurus_weinlandi_266684_La_Zurza .G..T...... C...... A. [180] C.stenurus_weinlandi_266685_La_Zurza .G..T...... C...... A. [180] D.bilobatus_194940_Costa_Rica ...... TG....A ...... C. .C...... A.A. [180] D.nigropunctatus_191015_San_Luis_de_Potosi ....T...... AG...... C. .C..C...... A. [180]

116

D.nigropunctatus_191319_Los_Calderos ....T...... AG...... C. ....C...... A. [180] D.nigropunctatus_191320_Los_Calderos ....T...... AG...... C. ....C...... A. [180] D.nigropunctatus_191542_San_Vincente ...... AG...... C. .C...... A. [180] D.garridoi_193507_El_Manguito ....T...... AG...... C. .C..C...... A. [180] D.pleii_191574_Rio_Abajo ...... G..AG....T ...... C..C...... A. [180] C.barbouri_161120_Quick_Step .G...... C. .C.....G.. ...A. [180] C.barbouri_190678_Tejunde .G...... C. .C.....G.. ...A. [180] C.crusculus_101571_Ewarton ...... C. .C.....G.. ...A. [180] C.crusculus_101572_Content ...... C. .C.....G.. ...A. [180] C.crusculus_172442_Duncans ...... G.....C...... G.. ...A. [180] C.crusculus_101554_Town_Head ...... T..C. .C.....G.. ...A. [180] C.crusculus_103448_Knoxwood ...... T..C. .C...... A. [180] C.crusculus_103449_Knowxwood ...... T..C. .C...... A. [180] C.crusculus_103460_Jackson_Bay ...... C. .C.....G.. ...A. [180] C.crusculus_103586_Jackson_Bay ...... G.....C...... G.. ...A. [180] C.crusculus_161123_Duncans .G...... C. .C.....G.. ...A. [180] C.crusculus_161124_Salt_Gut ...... G.....C...... G.. ...A. [180] C.crusculus_171439_Oracabessa ...... G.....C...... G.. ...A. [180] C.crusculus_171601_Port_Maria ...... G.....C...... G.. ...A. [180] C.crusculus_171603_Port_Maria ...... G.....C...... G.. ...A. [180] C.crusculus_171604_Port_Maria ...... G.....C...... G.. ...A. [180] C.crusculus_171605_Port_Maria ...... G.....C...... G.. ...A. [180] C.crusculus_171606_Oracabessa ...... G.....C...... G.. ...A. [180] C.crusculus_171607_Braeton .G...... C. .C.....G.. ...A. [180]

117

C.crusculus_172438_Oracabessa ...... G.....C...... G.. ...A. [180] C.crusculus_172441_Old_Hope .G...... T..C. .C...... A. [180] C.crusculus_172465_Section .G...... C. .C...... A. [180] C.duquesneyi_267952_Hellshire_Hills ...... T..C. .C...... A. [180] C.hewardii_267097_Mandeville .G...... C. .C...... A. [180] C.crusculus_101573_Hanover_Content ...... G.....C...... G.. ...A. [180] C.crusculus_101615_Oracabessa ...... C. .C.....G.. ...A. [180] C.crusculus_172439_Duncans ...... G.....C...... G.. ...A. [180] C.crusculus_172440_Old_Hope .G...... C. .C.....G.. ...A. [180] C.crusculus_190695_Duncans ...... T..C. .C...... A. [180] C.sepsoides_193116_Majagual ...... T .....T...... GA.A. [180] C.sepsoides_193117_Majagual ...... T .....T...... GA.A. [180] C.sepsoides_193118_Majagual ...... T .....T...... GA.A. [180] C.sepsoides_193202_Tenares ...... T .....T...... GA.A. [180] C.sepsoides_193203_Tenares ...... T .....T...... GA.A. [180] C.sepsoides_193204_Tenares ...... T .....T...... GA.A. [180] C.sepsoides_193040_Cotui ...... T .....T...... GA.A. [180] C.sepsoides_193041_Cotui ...... T .....T...... GA.A. [180] C.sepsoides_193042_Cotui ...... T .....T...... GA.A. [180] C.sepsoides_191851_Pestel ...... T .....T...... GA.A. [180] C.sepsoides_191914_Marche_Leon ....T...... T..C. .C...... TA.A. [180] C.sepsoides_191915_Marche_Leon ...... T.... .C...... A.A. [180] C.sepsoides_192313_Baraderes ...... G...... T.... .C...... A.A. [180] D.pleii_191575_Rio_Abajo ...... G..AG....T ...... C..C...... A. [180]

118

D.pleii_191576_Rio_Abajo ...... G..AG....T ...... C..C...... A. [180] O.apodus_194938 ....A...... A..A ...... C. .C...... A.A. [180] C.agasepsoides_160191_Canoa CATCCGCCCT TATCCTGTTC TCAAGCACAC TTAATGCCCA AAGCA [225] C.agasepsoides_194829_Bucan_Detwi ...... [225] C.agasepsoides_194830_Bucan_Detwi ...... [225] C.curtissi_aporus_102609_La_Descubierta ...... A.....A...... T... .A..C...... [225] C.curtissi_aporus_102610_La_Descubierta ...... A.....A...... T... .C..C...... [225] C.curtissi_aporus_102611_La_Descubierta ...... A.....A...... T... .A..C...... [225] C.curtissi_aporus_102706_Barahona ...... A.....A...... T... .C..C...... C.. [225] C.curtissi_aporus_102707_Juancho ...... A.....A...... T... .C..C...... [225] C.curtissi_aporus_102709_Juancho ...... A.....A...... T... .C..C...... [225] C.curtissi_aporus_102716_Barahona ...... A.....A...... T... .C..C...... C.. [225] C.curtissi_aporus_102915_Los_Arroyos ...... A.....A...... T... .C..C...... [225] C.curtissi_aporus_102930_Barahona ...... A.....A...... T... .C..C...... C.. [225] C.curtissi_aporus_194831_Bucan_Detwi ...... A.....A...... T... .A..C...... [225] C.curtissi_aporus_194832_Bucan_Detwi ...... A.....A...... T... .C..C...... [225] C.curtissi_aporus_194833_Bucan_Detwi ...... A.....A...... T... .C..C...... [225] C.curtissi_aporus_194838_Bucan_Detwi ...... A.....A...... T... .A..C...... [225] C.badius_194964_Navassa_Island ...... A. A.....A...... TT...... C.. [225] C.costatus_costatus_103085_Castillion ....T..... A...... T... .C..C...... [225] C.costatus_costatus_104113_Castillion ....T..... A...... T... .C..C...... [225] C.costatus_costatus_192054_Abricots ....T..... A...... T... .C..C...... [225] C.costatus_costatus_192260_Beaumont ....T..... A...... T... .C..C...... [225] C.cyanochloris_194930_Costa_Rica .C...... A. A..TT.A..T .....T.T.A .C...... GAA. [225]

119

C.curtissi_curtissi_194503_La_Descubierta ...... A. A...... T... .C..C...... G... [225] C.darlingtoni_161686_Cacique_Enriquillo ....A..... A.....A..T ...... T...... C...... [225] C.darlingtoni_161687_Cacique_Enriquillo ....A..... A.....A..T ...... T...... C...... [225] C.darlingtoni_161688_Cacique_Enriquillo ....A..... A.....A..T ...... T...... C...... [225] C.curtissi_diastatus_194553_Mole_St._Nicolas ...... A.....C...... T... .C..C..T...... [225] C.curtissi_diastatus_194580_Mole_St._Nicolas ...... A.....C...... T... .C..C..T...... [225] C.enneagrammus_191044_Mexico ....T...T. A.....A...... T.A ...... A.T .TA.. [225] C.maculatus_266550_Cayman_Brac_West ...... A. A.....A...... T...... C.. [225] C.maculatus_266551_Cayman_Brac_West ...... A. A.....A...... T...... C.. [225] C.maculatus_266552_Cayman_Brac_West ...... A. A.....A...... T...... C.. [225] C.maculatus_266553_Cayman_Brac_West ...... A. A.....A...... T...... C.. [225] C.maculatus_266527_Cayman_Brac_West ...... A. A.....A...... T...... C.. [225] C.marcanoi_193279_Valle_de_Bao ...... A.....T...... T... .C..C...... [225] C.marcanoi_193280_Valle_de_Bao ...... A.....T...... T... .C..C...... [225] C.marcanoi_193286_Valle_de_Bao ...... A.....T...... T... .C..C...... [225] C.marcanoi_193309_Valle_de_Bao ...... A.....T...... T... .C..C...... [225] C.marcanoi_193310_Valle_de_Bao ...... A.....T...... T... .C..C...... [225] C.haetiana_mylicus_102546_Cabral ...... A.....A...... T...... [225] C.haetiana_mylicus_102547_Cabral ...... A.....A...... T...... [225] C.haetiana_mylicus_102550_Cabral ...... A.....A...... T...... [225] C.haetiana_mylicus_160201_Tejunde ...... A.....A...... T...... [225] C.costatus_neiba_102922_La_Descubierta ...... A.....C...... T... .C..C...... [225] C.costatus_neiba_102923_Los_Pinos ...... A.....C...... T... .C..C...... [225] C.costatus_neiba_102924_Los_Pinos ...... A.....C...... T... .C..C...... [225]

120

C.costatus_neiba_102925_Los_Pinos ...... A.....C...... T... .C..C...... [225] C.costatus_neiba_161498_Cacique_Enriquillo ...... A.....C...... T... .C..C...... [225] C._costatus_neiba_194362_Apolinar_Pelodroma ...... A.....C...... T... .C..C...... [225] C.costatus_neiba_194363_Apolinar_Pelodroma ...... A.....C...... T... .C..C...... [225] C.costatus_neiba_266321_Rosa_de_La_Pierda ...... A.....C...... T... .C..C...... [225] C.costatus_neiba_266683_Rosa_de_La_Pierda ...... A.....C...... T... .C..C...... [225] C.costatus_oreistes_104408_Jacmel ....A..... A...... T... .A..C...... [225] C.costatus_oreistes_192480_Puerto_Escondido ....A..... A...... T... .A..C...... [225] C.sepsoides_102369_Sabana_de_La_Mar ...... C...... T...... T.. [225] C.sepsoides_191850_Pestel ...... C.....A...... T...... T.. [225] C.sepsoides_191913_Marche_Leon ...... C.....A...... T...... T.. [225] C.sepsoides_192469_La_Zanga ...... C.....A...... T...... T.. [225] C.sepsoides_193201_Tenares ...... C.....A...... T...... T.. [225] C.sepsoides_266748_Jacmel ...... C.....A...... T...... T.. [225] C.sepsoides_266749_Jacmel ...... C.....A...... T...... T.. [225] C.stenurus_stenurus_103900_Rampe_des_Lions_Boios_Sec ...... A. A...T.A...... T... .C..C...... C.. [225] C.stenurus_stenurus_103901_Rampe_des_Lions_Boios_Sec ...... A. A...T.A...... T... .C..C...... C.. [225] C.stenurus_stenurus_103902_Rampe_des_Lions_Boios_Sec ...... A. A...T.A...... T... .C..C...... C.. [225] C.stenurus_stenurus_267494_lle_a_Vache ...... A. A...T.A...... T... .C..C...... C.. [225] C.haetiana_surdus_104418_Gros_Cheval ...... A.....A...... T...... A.. [225] C.haetiana_surdus_104419_Gros_Cheval ...... A.....A...... T...... A.. [225] C.haetiana_surdus_192486_Puerto_Escondido ...... A...... T...... [225] C.haetiana_surdus_192497_Puerto_Escondido ...... A...... T...... [225] C.stenurus_rugosus_101247_Sabana_de_La_Mar ...... A. A...T...... T...... C.. [225]

121

C.stenurus_rugosus_101248_Sabana_de_La_Mar ...... A. A...T...... T...... C.. [225] C.stenurus_rugosus_102371_El_Valle ...... A. A...T...... T...... C.. [225] C.stenurus_rugosus_103075_Las_Galeras ...... A. A...T...... T...... C.. [225] C.stenurus_rugosus_103083_Nagua ...... A. A...T...... T...... C.. [225] C.stenurus_rugosus_103084_Nagua ...... A. A...T...... T...... C.. [225] C.stenurus_rugosus_193191_San_Francisco_de_Macoris ...... A. A...T...... T...... C.. [225] C.stenurus_rugosus_193192_San_Francisco_de_Macoris ...... A. A...T...... T...... C.. [225] C.stenurus_rugosus_193197_Tenares ...... A. A...T...... T...... C.. [225] C.stenurus_rugosus_193198_Tenares ...... A. A...T...... T...... C.. [225] C.stenurus_rugosus_193216_Tenares ...... A. A...T...... T...... C.. [225] C.stenurus_rugosus_193217_Tenares ...... A. A...T...... T...... C.. [225] C.warreni_172914_Hispaniola ....A..... A...T.A...... T...... [225] C.warreni_194521_Puerto_Plata ....A..... A...T.A...... T...... [225] C.stenurus_weinlandi_102712_Canoa ...... TA. A..TT.A...... T...... C.. [225] C.stenurus_weinlandi_103607_Soliette ...... A. A...T.A...... T...... C.. [225] C.stenurus_weinlandi_191591_Thomaseau ...... A. A...T.A...... T...... C.. [225] C.stenurus_weinlandi_191622_Thomaseau ...... A. A...T.A...... T...... C.. [225] C.stenurus_weinlandi_192405_Thomaseau ...... A. A...T.A...... T...... C.. [225] C.stenurus_weinlandi_266286_El_Azul ...... TA. A..TT.A...... T...... C.. [225] C.stenurus_weinlandi_266682_La_Zurza ...... TA. A..TT.A...... T...... C.. [225] C.stenurus_weinlandi_266684_La_Zurza ...... TA. A..TT.A...... T...... C.. [225] C.stenurus_weinlandi_266685_La_Zurza ...... TA. A..TT.A...... T...... C.. [225] D.bilobatus_194940_Costa_Rica .C...... A.....A...... T.A .C.....A.. ..C.. [225] D.nigropunctatus_191015_San_Luis_de_Potosi ...... A.. A...... G...... T.A C...C...... GAT. [225]

122

D.nigropunctatus_191319_Los_Calderos ...... A.. A...... G...... T.A C...C...... GAT. [225] D.nigropunctatus_191320_Los_Calderos ...... A.. A...... G...... T.A C...C...... GAT. [225] D.nigropunctatus_191542_San_Vincente ...... A.. A...... G...... T.A CC..C...... GAT. [225] D.garridoi_193507_El_Manguito ...... A.. A.....A... G...... T.A CC..C...... GA.. [225] D.pleii_191574_Rio_Abajo ....T..... A.....A... G...... T.A .C..C..TA. ..A.. [225] C.barbouri_161120_Quick_Step ...... A.....A...... TT...... C...... [225] C.barbouri_190678_Tejunde ...... A.....A...... TT...... C...... [225] C.crusculus_101571_Ewarton ...... G.....A...... TT...... C...... T. [225] C.crusculus_101572_Content ...... G.....A...... TT...... C...... T. [225] C.crusculus_172442_Duncans ...... A.....A...... TT...... C...... T. [225] C.crusculus_101554_Town_Head ...... G.....A...... TT...... C...... T. [225] C.crusculus_103448_Knoxwood .C...... A.....A...... T... .C..C...... [225] C.crusculus_103449_Knowxwood .C...... A.....A...... T... .C..C...... [225] C.crusculus_103460_Jackson_Bay ...... A.....A...... T...... C...... T. [225] C.crusculus_103586_Jackson_Bay ...... A.....A...... TT...... C...... T. [225] C.crusculus_161123_Duncans ...... A.....A...... T... .C..C...... [225] C.crusculus_161124_Salt_Gut ...... A.....A...... TT...... C...... T. [225] C.crusculus_171439_Oracabessa ...... A.....A...... TT...... C...... T. [225] C.crusculus_171601_Port_Maria ...... A.....A...... TT...... C...... T. [225] C.crusculus_171603_Port_Maria ...... A.....A...... TT...... C...... T. [225] C.crusculus_171604_Port_Maria ...... A.....A...... TT...... C...... T. [225] C.crusculus_171605_Port_Maria ...... A.....A...... TT...... C...... T. [225] C.crusculus_171606_Oracabessa ...... A.....A...... TT...... C...... T. [225] C.crusculus_171607_Braeton ...... A.....A...... T... .C..C...... [225]

123

C.crusculus_172438_Oracabessa ...... A.....A...... TT...... C...... T. [225] C.crusculus_172441_Old_Hope ...... A.....A...... T...... C...... A.. [225] C.crusculus_172465_Section ...... A. A.....A...... T...... C...... [225] C.duquesneyi_267952_Hellshire_Hills ....T..... A..T..A...... T... .C..C...... [225] C.hewardii_267097_Mandeville ...... A.....A...... T...... C...... [225] C.crusculus_101573_Hanover_Content ...... A.....A...... TT...... C...... T. [225] C.crusculus_101615_Oracabessa ...... G.....A...... TT...... C...... T. [225] C.crusculus_172439_Duncans ...... A.....A...... TT...... C...... T. [225] C.crusculus_172440_Old_Hope ...... A.....A...... T... .C..C...... [225] C.crusculus_190695_Duncans .C...... A.....A...... T... .C..C...... [225] C.sepsoides_193116_Majagual ...... C.....A...... T...... T.. [225] C.sepsoides_193117_Majagual ...... C.....A...... T...... T.. [225] C.sepsoides_193118_Majagual ...... C.....A...... T...... T.. [225] C.sepsoides_193202_Tenares ...... C.....A...... T...... T.. [225] C.sepsoides_193203_Tenares ...... C.....A...... T...... T.. [225] C.sepsoides_193204_Tenares ...... C.....A...... T...... T.. [225] C.sepsoides_193040_Cotui ...... C.....A...... T...... T.. [225] C.sepsoides_193041_Cotui ...... C.....A...... T...... T.. [225] C.sepsoides_193042_Cotui ...... C.....A...... T...... T.. [225] C.sepsoides_191851_Pestel ...... C.....A...... T...... T.. [225] C.sepsoides_191914_Marche_Leon ...... C.....A...... T..A ...... T.. ..TA. [225] C.sepsoides_191915_Marche_Leon ...... C.....A...... T...... C.. [225] C.sepsoides_192313_Baraderes ...... C.....A...... T...... T.. [225] D.pleii_191575_Rio_Abajo ....T..... A.....A... G...... T.A .C..C..TA. ..A.. [225]

124

D.pleii_191576_Rio_Abajo ....T..... A.....A... G...... T.A .C..C..TA. ..A.. [225] O.apodus_194938 ....T..... A.....C... ..C...... A .A...... TA. [225]

C.agasepsoides_160191_Canoa CTGGACAATG AAATATCCTA GACCTCAATA ACCAAACATC AAAAA [270] C.agasepsoides_194829_Bucan_Detwi ...... G. [270] C.agasepsoides_194830_Bucan_Detwi ...... G. [270] C.curtissi_aporus_102609_La_Descubierta .C.....G...... T..C .....T..C. .T....T...... [270] C.curtissi_aporus_102610_La_Descubierta .C.....G...... T..C .....T..C. .T....T...... [270] C.curtissi_aporus_102611_La_Descubierta .C.....G...... T..C .....T..C. .T....T...... [270] C.curtissi_aporus_102706_Barahona .C.....G...... T..C .....T..C. .T....T...... [270] C.curtissi_aporus_102707_Juancho .C.....G...... T..C .....T..C. .T....T...... [270] C.curtissi_aporus_102709_Juancho .C.....G...... T..C .....T..C. .T....T...... [270] C.curtissi_aporus_102716_Barahona .C.....G...... T..C .....T..C. .T....T...... [270] C.curtissi_aporus_102915_Los_Arroyos .C.....G...... T..C .....T..C. .T....T...... [270] C.curtissi_aporus_102930_Barahona .C.....G...... T..C .....T..C. .T....T...... [270] C.curtissi_aporus_194831_Bucan_Detwi .C.....G...... T..C .....T..C. .T....T...... [270] C.curtissi_aporus_194832_Bucan_Detwi .C.....G...... T..C .....T..C. .T....T...... [270] C.curtissi_aporus_194833_Bucan_Detwi .C.....G...... T..C .....T..C. .T....T...... [270] C.curtissi_aporus_194838_Bucan_Detwi .C.....G...... T..C .....T..C. .T....T...... [270] C.badius_194964_Navassa_Island .C...... G.C...... G.C...... G...... [270] C.costatus_costatus_103085_Castillion .C.....G...... C ...... C...... [270] C.costatus_costatus_104113_Castillion .C.....G...... C ...... C...... [270] C.costatus_costatus_192054_Abricots .C...... C ...... C...... [270] C.costatus_costatus_192260_Beaumont .C.....G...... T ...... C...... [270]

125

C.cyanochloris_194930_Costa_Rica .C..C...... C...A.. C.A..AG.AG C..C.CT.G. CC... [270] C.curtissi_curtissi_194503_La_Descubierta .C...... C.....C .....TG.C. .T....T...... [270] C.darlingtoni_161686_Cacique_Enriquillo .C...... T ...... C...... C.... [270] C.darlingtoni_161687_Cacique_Enriquillo .C...... T ..A.....C...... C.... [270] C.darlingtoni_161688_Cacique_Enriquillo .C...... T ...... C...... C.... [270] C.curtissi_diastatus_194553_Mole_St._Nicolas .C...... C .....T..C. .T....T...... [270] C.curtissi_diastatus_194580_Mole_St._Nicolas .C...... C .....T..C. .T....T...... [270] C.enneagrammus_191044_Mexico .C..C...... TA.. C.A..AG.A. GT.C.CT.G. CC... [270] C.maculatus_266550_Cayman_Brac_West .C..G...... T...G.C...... [270] C.maculatus_266551_Cayman_Brac_West .C..G...... T...G.C...... [270] C.maculatus_266552_Cayman_Brac_West .C..G...... T...G.C...... [270] C.maculatus_266553_Cayman_Brac_West .C..G...... T...G.C...... [270] C.maculatus_266527_Cayman_Brac_West .C..G...... T...G.C...... [270] C.marcanoi_193279_Valle_de_Bao .C.....G...... C ..T..T..C. .T....T...... [270] C.marcanoi_193280_Valle_de_Bao .C.....G...... C ..T..T..C. .T....T...... [270] C.marcanoi_193286_Valle_de_Bao .C.....G...... C ..T..T..C. .T....T...... [270] C.marcanoi_193309_Valle_de_Bao .C.....G...... C ..T..T..C. .T....T...... [270] C.marcanoi_193310_Valle_de_Bao .C.....G...... C ..T..T..C. .T....T...... [270] C.haetiana_mylicus_102546_Cabral ...... T...... T... C..G. [270] C.haetiana_mylicus_102547_Cabral ...... T...... T... C..G. [270] C.haetiana_mylicus_102550_Cabral ...... T...... T... C..G. [270] C.haetiana_mylicus_160201_Tejunde ...... T...... T... C..G. [270] C.costatus_neiba_102922_La_Descubierta .C.....G...... C .....T..C. .T....T...... [270] C.costatus_neiba_102923_Los_Pinos .C.....G...... C .....T..C. .T....T...... [270]

126

C.costatus_neiba_102924_Los_Pinos .C.....G...... C .....T..C. .T....T...... [270] C.costatus_neiba_102925_Los_Pinos .C.....G...... C .....T..C. .T....T...... [270] C.costatus_neiba_161498_Cacique_Enriquillo .C...... C .....T..C. .T....T...... [270] C._costatus_neiba_194362_Apolinar_Pelodroma .C...... C .....T..C. .T....T...... [270] C.costatus_neiba_194363_Apolinar_Pelodroma .C...... C .....T..C. .T....T...... [270] C.costatus_neiba_266321_Rosa_de_La_Pierda .C...... C .....T..C. .T....T...... [270] C.costatus_neiba_266683_Rosa_de_La_Pierda .C...... C .....T..C. .T....T...... [270] C.costatus_oreistes_104408_Jacmel ...... C .....A..C...... [270] C.costatus_oreistes_192480_Puerto_Escondido ...... C .....A..C...... [270] C.sepsoides_102369_Sabana_de_La_Mar .C.....G.. ...C...... T....T... C.... [270] C.sepsoides_191850_Pestel ...... C...... T...... C.... [270] C.sepsoides_191913_Marche_Leon ...... G.. ...C...... T....T... C.... [270] C.sepsoides_192469_La_Zanga .C.....G.. ...C...... T....T... C.... [270] C.sepsoides_193201_Tenares .C.....G.. ...C...... T....T... C.... [270] C.sepsoides_266748_Jacmel .C.....G.. ...C...... T....T... C.... [270] C.sepsoides_266749_Jacmel .C.....G.. ...C...... T....T... C.... [270] C.stenurus_stenurus_103900_Rampe_des_Lions_Boios_Sec .C...... C.....G ..T...G...... [270] C.stenurus_stenurus_103901_Rampe_des_Lions_Boios_Sec .C...... C.....G ..T...G...... [270] C.stenurus_stenurus_103902_Rampe_des_Lions_Boios_Sec .C...... C.....G ..T...G...... [270] C.stenurus_stenurus_267494_lle_a_Vache .C...... C.....G ..T...G...... [270] C.haetiana_surdus_104418_Gros_Cheval ...... C..T... ..T...... T... C..G. [270] C.haetiana_surdus_104419_Gros_Cheval ...... C..T... ..T...... T... C..G. [270] C.haetiana_surdus_192486_Puerto_Escondido ...... C..T... ..T...... G.T... C..G. [270] C.haetiana_surdus_192497_Puerto_Escondido ...... C..T... ..T...... G.T... C..G. [270]

127

C.stenurus_rugosus_101247_Sabana_de_La_Mar .C..G...... C...... T...G...... [270] C.stenurus_rugosus_101248_Sabana_de_La_Mar .C..G...... C...... T...G...... [270] C.stenurus_rugosus_102371_El_Valle .C..G...... C...... T...G...... [270] C.stenurus_rugosus_103075_Las_Galeras .C..G...... C...... T...G.C...... [270] C.stenurus_rugosus_103083_Nagua .C..G...... C...... T...G...... [270] C.stenurus_rugosus_103084_Nagua .C..G...... C...... T...G...... [270] C.stenurus_rugosus_193191_San_Francisco_de_Macoris .C..G...... C...... T...G...... [270] C.stenurus_rugosus_193192_San_Francisco_de_Macoris .C..G...... C...... T...G...... [270] C.stenurus_rugosus_193197_Tenares .C..G...... T...G...... [270] C.stenurus_rugosus_193198_Tenares .C..G...... C...... T...G...... [270] C.stenurus_rugosus_193216_Tenares .C..G...... T...G...... [270] C.stenurus_rugosus_193217_Tenares .C..G...... C...... T...G...... [270] C.warreni_172914_Hispaniola .C...... G.C.....T ...... C...... C.... [270] C.warreni_194521_Puerto_Plata .C...... G.C.....T ...... C...... C.... [270] C.stenurus_weinlandi_102712_Canoa .C...... C...... T...G...... [270] C.stenurus_weinlandi_103607_Soliette .C...... C...... T...G...... [270] C.stenurus_weinlandi_191591_Thomaseau .C...... C...... T...G...... [270] C.stenurus_weinlandi_191622_Thomaseau .C...... C...... T...G...... [270] C.stenurus_weinlandi_192405_Thomaseau .C...... C...... T...G...... [270] C.stenurus_weinlandi_266286_El_Azul .C...... C...... T...G...... [270] C.stenurus_weinlandi_266682_La_Zurza .C...... C...... T...G...... [270] C.stenurus_weinlandi_266684_La_Zurza .C...... C...... T...G...... [270] C.stenurus_weinlandi_266685_La_Zurza .C...... C...... T...G...... [270] D.bilobatus_194940_Costa_Rica .A...... C C.AT.AG.A. ...C.TTCG. CC... [270]

128

D.nigropunctatus_191015_San_Luis_de_Potosi .A...... C...ACC C.A..AG.A. G..CGCT.G. .C... [270] D.nigropunctatus_191319_Los_Calderos .A...... C...ACC C.A..AG.A. G..CGCT.G. .C... [270] D.nigropunctatus_191320_Los_Calderos .A...... C...ACC C.A..AG.A. G..CGCT.G. .C... [270] D.nigropunctatus_191542_San_Vincente .A...... C...ACC C.A..AG.A. G..CG.T.G. .C... [270] D.garridoi_193507_El_Manguito .A...... C...ACC C.A..AG.A. ...C.CT.G. .C... [270] D.pleii_191574_Rio_Abajo .A...... C...ACC C.A..AG.A. ...CCCT... .C... [270] C.barbouri_161120_Quick_Step .C...... TA...... T...... [270] C.barbouri_190678_Tejunde .C.....G...... TA...... T...... [270] C.crusculus_101571_Ewarton .C...... T..G .....T...... [270] C.crusculus_101572_Content .C...... T..G .....T...... [270] C.crusculus_172442_Duncans .C...... GG....TA...... T...... [270] C.crusculus_101554_Town_Head .C...... T..G .....T...... [270] C.crusculus_103448_Knoxwood .C...... T...... T..C...... [270] C.crusculus_103449_Knowxwood .C...... T...... T..C...... [270] C.crusculus_103460_Jackson_Bay .C.....G.. G.....TA...... T...... [270] C.crusculus_103586_Jackson_Bay .C...... G..C..TA...... T...... [270] C.crusculus_161123_Duncans .A...... C..T... ..T..T...... [270] C.crusculus_161124_Salt_Gut .C...... G.....TA...... T...... [270] C.crusculus_171439_Oracabessa .C...... G.....TA...... T...... [270] C.crusculus_171601_Port_Maria .C...... G.....TA...... T...... [270] C.crusculus_171603_Port_Maria .C...... G.....TA...... T...... [270] C.crusculus_171604_Port_Maria .C...... G.....TA...... T...... [270] C.crusculus_171605_Port_Maria .C...... G.....TA...... T...... [270] C.crusculus_171606_Oracabessa .C...... G.....TA...... T...... [270]

129

C.crusculus_171607_Braeton .A...... C..T...... T...... [270] C.crusculus_172438_Oracabessa .C...... G.....TA...... T...... [270] C.crusculus_172441_Old_Hope .C...... G...... T..C...... [270] C.crusculus_172465_Section .C...... GG....T...... T...... [270] C.duquesneyi_267952_Hellshire_Hills .C...... T..C...... [270] C.hewardii_267097_Mandeville .C...... T..C...... [270] C.crusculus_101573_Hanover_Content .C.....G.. G.....TA...... T...... [270] C.crusculus_101615_Oracabessa .C...... T..G .....T...... [270] C.crusculus_172439_Duncans .C...... G.....TA...... T...... [270] C.crusculus_172440_Old_Hope .A...... C..T... ..T..T...... [270] C.crusculus_190695_Duncans .C...... T...... T..C...... [270] C.sepsoides_193116_Majagual .C.....G.. ...C...... T....T... C.... [270] C.sepsoides_193117_Majagual .C.....G.. ...C...... T....T... C.... [270] C.sepsoides_193118_Majagual .C.....G.. ...C...... T....T... C.... [270] C.sepsoides_193202_Tenares .C.....G.. ...C...... T....T... C.... [270] C.sepsoides_193203_Tenares .C.....G.. ...C...... T...... T....T... C.... [270] C.sepsoides_193204_Tenares .C.....G.. ...C...... T....T... C.... [270] C.sepsoides_193040_Cotui .C.....G.. ...C...... T....T... C.... [270] C.sepsoides_193041_Cotui .C.....G.. ...C...... T....T... C.... [270] C.sepsoides_193042_Cotui .C.....G.. ...C...... T....T... C.... [270] C.sepsoides_191851_Pestel .C.....G.. ...C...... T....T... C.... [270] C.sepsoides_191914_Marche_Leon .C...... GG.C...... T.....C. .T...... C.... [270] C.sepsoides_191915_Marche_Leon .C...... C...... T...... C.... [270] C.sepsoides_192313_Baraderes ...... G.. ...C...... T....T... C.... [270]

130

D.pleii_191575_Rio_Abajo .A...... C...ACC C.A..AG.A. ...CCCT... .C... [270] D.pleii_191576_Rio_Abajo .A...... C...ACC C.A..AG.A. ...CCCT... .C... [270] O.apodus_194938 .C..C..... G.GC...... C.A..AG.AG ...CGCT.G. CC... [270] C.agasepsoides_160191_Canoa CACTAATTAC TATAGCACTG GGAATGAAAC TAGGACTTGC ACCAG [315] C.agasepsoides_194829_Bucan_Detwi ...... [315] C.agasepsoides_194830_Bucan_Detwi ...... [315] C.curtissi_aporus_102609_La_Descubierta ..T...... C...... A...... [315] C.curtissi_aporus_102610_La_Descubierta ..T...... C...... A...... [315] C.curtissi_aporus_102611_La_Descubierta ..T...... C...... A...... [315] C.curtissi_aporus_102706_Barahona ..T...... C...... A...... [315] C.curtissi_aporus_102707_Juancho ..T...... C...... A...... [315] C.curtissi_aporus_102709_Juancho ..T...... C...... A...... [315] C.curtissi_aporus_102716_Barahona ..T...... C...... A...... [315] C.curtissi_aporus_102915_Los_Arroyos ..T...... C...... A...... [315] C.curtissi_aporus_102930_Barahona ..T...... C...... A...... [315] C.curtissi_aporus_194831_Bucan_Detwi ..T...... C...... A...... [315] C.curtissi_aporus_194832_Bucan_Detwi ..T...... C...... A...... [315] C.curtissi_aporus_194833_Bucan_Detwi ..T...... C...... A...... [315] C.curtissi_aporus_194838_Bucan_Detwi ..T...... C...... A...... [315] C.badius_194964_Navassa_Island ...... CGC...... A .....A..G...... [315] C.costatus_costatus_103085_Castillion ..T...... C...... A ..G..A.... .T...... [315] C.costatus_costatus_104113_Castillion ..T...... C...... A ..G..A.... .T...... [315] C.costatus_costatus_192054_Abricots ..T...... C...... A ..G..A..G. .T...... [315] C.costatus_costatus_192260_Beaumont ..T...... C...... A ..G..A.... .T...... [315]

131

C.cyanochloris_194930_Costa_Rica TC..TC.... C...... A .CC..A...... A.. C.... [315] C.curtissi_curtissi_194503_La_Descubierta ..T...... C...... A...... [315] C.darlingtoni_161686_Cacique_Enriquillo ...... C.....C..A .....A.... .G.....C.. C.... [315] C.darlingtoni_161687_Cacique_Enriquillo ...... C.....C..A .....A.... .G.....C.. C.... [315] C.darlingtoni_161688_Cacique_Enriquillo ...... C.....C..A .....A.... .G.....C.. C.... [315] C.curtissi_diastatus_194553_Mole_St._Nicolas ...... C...... A...... [315] C.curtissi_diastatus_194580_Mole_St._Nicolas ...... C...... A...... [315] C.enneagrammus_191044_Mexico TC...C.A.. C...... T .CC..A...... GT.A...... [315] C.maculatus_266550_Cayman_Brac_West ..T...... C.C...... A .....A..GT ...... [315] C.maculatus_266551_Cayman_Brac_West ..T...... C.C...... A .....A..GT ...... [315] C.maculatus_266552_Cayman_Brac_West ..T...... C.C...... A .....A..GT ...... [315] C.maculatus_266553_Cayman_Brac_West ..T...... C.C...... A .....A..GT ...... [315] C.maculatus_266527_Cayman_Brac_West ..T...... C.C...... A .....A..GT ...... [315] C.marcanoi_193279_Valle_de_Bao ...... C...... A...... G...... [315] C.marcanoi_193280_Valle_de_Bao ...... C...... A...... G...... [315] C.marcanoi_193286_Valle_de_Bao ...... C...... A...... G...... [315] C.marcanoi_193309_Valle_de_Bao ...... C...... A...... G...... [315] C.marcanoi_193310_Valle_de_Bao ...... C...... A...... G...... [315] C.haetiana_mylicus_102546_Cabral ..T...... C...... A .....A...... [315] C.haetiana_mylicus_102547_Cabral ..T...... C...... A .....A...... [315] C.haetiana_mylicus_102550_Cabral ..T...... C...... A .....A...... [315] C.haetiana_mylicus_160201_Tejunde ..T...... C...... A .....A...... [315] C.costatus_neiba_102922_La_Descubierta ..T...... C...... A...... [315] C.costatus_neiba_102923_Los_Pinos ..T...... C...... A...... [315]

132

C.costatus_neiba_102924_Los_Pinos ..T...... C...... A...... [315] C.costatus_neiba_102925_Los_Pinos ..T...... C...... A...... [315] C.costatus_neiba_161498_Cacique_Enriquillo ..T...... C...... A .....A...... [315] C._costatus_neiba_194362_Apolinar_Pelodroma ..T...... C...... A .....A...... [315] C.costatus_neiba_194363_Apolinar_Pelodroma ..T...... C...... A .....A...... [315] C.costatus_neiba_266321_Rosa_de_La_Pierda ..T...... C...... A .....A...... [315] C.costatus_neiba_266683_Rosa_de_La_Pierda ..T...... C...... A .....A...... [315] C.costatus_oreistes_104408_Jacmel ..T...... C...... A .....A...... [315] C.costatus_oreistes_192480_Puerto_Escondido ..T...... C...... A .....A...... [315] C.sepsoides_102369_Sabana_de_La_Mar ...... C.. C..G..G..A .....A...... [315] C.sepsoides_191850_Pestel ...... A .....A...... [315] C.sepsoides_191913_Marche_Leon ...... A .....A...... [315] C.sepsoides_192469_La_Zanga ...... C.. C..G..G..A .....A...... G...... [315] C.sepsoides_193201_Tenares ...... C.. C..G..G..A .....A...... [315] C.sepsoides_266748_Jacmel ...... C.. C..G.....A .....A...... [315] C.sepsoides_266749_Jacmel ...... C.. C..G..G..A .....A...... [315] C.stenurus_stenurus_103900_Rampe_des_Lions_Boios_Sec .....G.... CGC...... A .....A..G...... [315] C.stenurus_stenurus_103901_Rampe_des_Lions_Boios_Sec .....G.... CGC...... A .....A..G...... [315] C.stenurus_stenurus_103902_Rampe_des_Lions_Boios_Sec .....G.... CGC...... A .....A..G...... [315] C.stenurus_stenurus_267494_lle_a_Vache .....G.... CGC...... A .....A..G...... [315] C.haetiana_surdus_104418_Gros_Cheval ..T...... C...... A ...... T ...... [315] C.haetiana_surdus_104419_Gros_Cheval ..T...... C...... A ...... T ...... [315] C.haetiana_surdus_192486_Puerto_Escondido ..T...... C...... A ...... [315] C.haetiana_surdus_192497_Puerto_Escondido ..T...... C...... A ...... [315]

133

C.stenurus_rugosus_101247_Sabana_de_La_Mar ...... C.C...... A .....A..GT ...... [315] C.stenurus_rugosus_101248_Sabana_de_La_Mar ...... C.C...... A .....A..GT ...... [315] C.stenurus_rugosus_102371_El_Valle ...... C.C...... A .....A..GT ...... [315] C.stenurus_rugosus_103075_Las_Galeras ...... C.C...... A .....A..GT ...... [315] C.stenurus_rugosus_103083_Nagua ...... C.C...... A .....A..GT ...... [315] C.stenurus_rugosus_103084_Nagua ...... C.C...... A .....A..GT ...... [315] C.stenurus_rugosus_193191_San_Francisco_de_Macoris ...... C.C...... A .....A..GT ...... [315] C.stenurus_rugosus_193192_San_Francisco_de_Macoris ...... C.C...... A .....A..GT ...... [315] C.stenurus_rugosus_193197_Tenares ...... C.C...... A .....A..GT ...... [315] C.stenurus_rugosus_193198_Tenares ...... C.C...... A .....A..GT ...... [315] C.stenurus_rugosus_193216_Tenares ...... C.C...... A .....A..GT ...... [315] C.stenurus_rugosus_193217_Tenares ...... C.C...... A .....A..GT ...... [315] C.warreni_172914_Hispaniola ...... C..G.....A .....A...... [315] C.warreni_194521_Puerto_Plata ...... C..G.....A .....A...... [315] C.stenurus_weinlandi_102712_Canoa ...... C.C...... A .....A..GT ...... [315] C.stenurus_weinlandi_103607_Soliette ...... C.C...... A .....A..GT ...... [315] C.stenurus_weinlandi_191591_Thomaseau ...... C.C...... A .....A..GT ...... [315] C.stenurus_weinlandi_191622_Thomaseau ...... C.C...... A .....A..GT ...... [315] C.stenurus_weinlandi_192405_Thomaseau ...... C.C...... A .....A..GT ...... [315] C.stenurus_weinlandi_266286_El_Azul ...... C.C...... A .....A..GT ...... [315] C.stenurus_weinlandi_266682_La_Zurza ...... C.C...... A .....A..GT ...... [315] C.stenurus_weinlandi_266684_La_Zurza ...... C.C...... A .....A..GT ...... [315] C.stenurus_weinlandi_266685_La_Zurza ...... C.C...... A .....A..GT ...... [315] D.bilobatus_194940_Costa_Rica TTTGCC.A.. C..G..TT.A TCT..A...... T.A...... [315]

134

D.nigropunctatus_191015_San_Luis_de_Potosi ....C..AT. ...G.....A .CC..A...... A.. ...CA [315] D.nigropunctatus_191319_Los_Calderos ....C..AT. ...G.....A .CC..A...... A.. ...CA [315] D.nigropunctatus_191320_Los_Calderos ....C..AT. ...G.....A .CC..A...... A.. ...CA [315] D.nigropunctatus_191542_San_Vincente ....C..AT...... A .CC..A...... A.. ...CA [315] D.garridoi_193507_El_Manguito ....C..AT. A...... A .CC..A...... A.. ...C. [315] D.pleii_191574_Rio_Abajo ....T..AT...... C..A .CT..A...T ...... A.. C...A [315] C.barbouri_161120_Quick_Step ..T....C.. C..G...... A...... T. [315] C.barbouri_190678_Tejunde ..T....C.. C..G...... A...... T. [315] C.crusculus_101571_Ewarton ..T....C.. CT...... A .....A...... T. [315] C.crusculus_101572_Content ..T....C.. CT.C.....A .....A...... T. [315] C.crusculus_172442_Duncans ..T....C.. CT...... A .....A...... T. [315] C.crusculus_101554_Town_Head ..T....C.. CT...... A .....A...... T. [315] C.crusculus_103448_Knoxwood ..T....C.. C..G..C..A .....A...... A.. ...T. [315] C.crusculus_103449_Knowxwood ..T....C.. C..G..C..A .....A...... T. [315] C.crusculus_103460_Jackson_Bay ..T..G.C.. CT...... A .....A...... T. [315] C.crusculus_103586_Jackson_Bay ..T....C.. CT...... A .....A...... C. [315] C.crusculus_161123_Duncans ...... C.. C...... T.A .....A...... T. [315] C.crusculus_161124_Salt_Gut ..T....C.. CT...... A .....A...... T. [315] C.crusculus_171439_Oracabessa ..T....C.. CT...... A .....A...... T. [315] C.crusculus_171601_Port_Maria ..T....C.. CT...... A .....A...... T. [315] C.crusculus_171603_Port_Maria ..T....C.. CT...... A .....A...... T. [315] C.crusculus_171604_Port_Maria ..T....C.. CT...... A .....A...... T. [315] C.crusculus_171605_Port_Maria ..T....C.. CT...... A .....A...... T. [315] C.crusculus_171606_Oracabessa ..T....C.. CT...... A .....A...... T. [315]

135

C.crusculus_171607_Braeton ..T....C.. C..G...T.A .....A...... C. [315] C.crusculus_172438_Oracabessa ..T....C.. CT...... A .....A...... T. [315] C.crusculus_172441_Old_Hope ..T....C.. ...G.....A .....A...... C.. ...T. [315] C.crusculus_172465_Section ..T....C.. C.....T..A .....A...... G.. ...T. [315] C.duquesneyi_267952_Hellshire_Hills ..T....C.. C..G.....A .....A...... C..... G.... [315] C.hewardii_267097_Mandeville ..T....C.. CC.G.....A .....A...... T. [315] C.crusculus_101573_Hanover_Content ..T....C.. CT...... A .....A...... T. [315] C.crusculus_101615_Oracabessa ..T....C.. CT...... A .....A...... T. [315] C.crusculus_172439_Duncans ..T....C.. CT...... A .....A...... T. [315] C.crusculus_172440_Old_Hope ...... C.. C...... T.A .....A...... T. [315] C.crusculus_190695_Duncans ..T....C.. C..G..C..A .....A...... T. [315] C.sepsoides_193116_Majagual ...... C.. C..G..G..A ...... [315] C.sepsoides_193117_Majagual ...... C.. C..G..G..A ...... [315] C.sepsoides_193118_Majagual ...... C.. C..G..G..A .....A...... [315] C.sepsoides_193202_Tenares ...... C.. C..G..G..A .....A...... [315] C.sepsoides_193203_Tenares ...... C.. C..G..G..A .....A...... [315] C.sepsoides_193204_Tenares ...... C.. C..G..G..A .....A...... [315] C.sepsoides_193040_Cotui ...... C.. C..G..G..A .....A...... [315] C.sepsoides_193041_Cotui ...... C.. C..G..G..A ...... [315] C.sepsoides_193042_Cotui ...... C.. C..G..G..A .....A...... [315] C.sepsoides_191851_Pestel ...... C.. C..G..G..A ...... [315] C.sepsoides_191914_Marche_Leon T...... C...... A ..G..A...... T ..... [315] C.sepsoides_191915_Marche_Leon ...... A .....A...... [315] C.sepsoides_192313_Baraderes ...... A .....A...... [315]

136

D.pleii_191575_Rio_Abajo ....T..AT...... C..A .CT..A...T ...... A.. C...A [315] D.pleii_191576_Rio_Abajo ....T..AT...... C..A .CT..A...T ...... A.. C...A [315] O.apodus_194938 ....TC.A.. .G.C..C..A .C...A..G...... A.. C..C. [315] C.agasepsoides_160191_Canoa CCCATTTCTG ACTTCCAGAA GTACTACAAG GAGCCTCACT TACAA [360] C.agasepsoides_194829_Bucan_Detwi ....C...... ------[360] C.agasepsoides_194830_Bucan_Detwi ....C...... ------[360] C.curtissi_aporus_102609_La_Descubierta ....C...... ------[360] C.curtissi_aporus_102610_La_Descubierta ....C...... C...... T...... T...... [360] C.curtissi_aporus_102611_La_Descubierta ....C...... ------[360] C.curtissi_aporus_102706_Barahona ....C...... ------[360] C.curtissi_aporus_102707_Juancho ....C...... ------[360] C.curtissi_aporus_102709_Juancho ....C...... ------[360] C.curtissi_aporus_102716_Barahona ....C...... ------[360] C.curtissi_aporus_102915_Los_Arroyos ....C...... ------[360] C.curtissi_aporus_102930_Barahona ....C...... C...... T...... T...... [360] C.curtissi_aporus_194831_Bucan_Detwi ....C...... C...... T...... T...... [360] C.curtissi_aporus_194832_Bucan_Detwi ....C...... ------[360] C.curtissi_aporus_194833_Bucan_Detwi ....C...... ------[360] C.curtissi_aporus_194838_Bucan_Detwi ....C...... C...... T...... T...... [360] C.badius_194964_Navassa_Island ....C...... T...... G...... [360] C.costatus_costatus_103085_Castillion ....C...... ------[360] C.costatus_costatus_104113_Castillion ....C...... C...... T...... T...... [360] C.costatus_costatus_192054_Abricots ....C...... C...... GT...... G.....T...... [360] C.costatus_costatus_192260_Beaumont ....C...... ------[360]

137

C.cyanochloris_194930_Costa_Rica .A..C...... A...... C..T.... .G.....CA. A.A.. [360] C.curtissi_curtissi_194503_La_Descubierta ....C..T.. ...C...... T...... T...... [360] C.darlingtoni_161686_Cacique_Enriquillo ....C...... C...... C...... [360] C.darlingtoni_161687_Cacique_Enriquillo ....C..... G..C...... C...... [360] C.darlingtoni_161688_Cacique_Enriquillo ....C..... G..C...... C...... [360] C.curtissi_diastatus_194553_Mole_St._Nicolas ....C...... C...... T...... [360] C.curtissi_diastatus_194580_Mole_St._Nicolas ....C...... ------[360] C.enneagrammus_191044_Mexico .A..C...... A...... C...... G.....C.. A.A.. [360] C.maculatus_266550_Cayman_Brac_West ....C...... ------[360] C.maculatus_266551_Cayman_Brac_West ....C...... ------[360] C.maculatus_266552_Cayman_Brac_West ....C...... ------[360] C.maculatus_266553_Cayman_Brac_West ....C...... ------[360] C.maculatus_266527_Cayman_Brac_West ....C...... G ...T...... [360] C.marcanoi_193279_Valle_de_Bao ....C...... C...... TT...... T...... [360] C.marcanoi_193280_Valle_de_Bao ....C...... ------[360] C.marcanoi_193286_Valle_de_Bao ....C...... C...... TT...... T...... [360] C.marcanoi_193309_Valle_de_Bao ....C...... ------[360] C.marcanoi_193310_Valle_de_Bao ....C...... ------[360] C.haetiana_mylicus_102546_Cabral ....C...... [360] C.haetiana_mylicus_102547_Cabral ....C...... ------[360] C.haetiana_mylicus_102550_Cabral ....C...... ------[360] C.haetiana_mylicus_160201_Tejunde ....C...... ------[360] C.costatus_neiba_102922_La_Descubierta ....C...... C...... T...... T...... [360] C.costatus_neiba_102923_Los_Pinos ....C...... ------[360]

138

C.costatus_neiba_102924_Los_Pinos ....C...... ------[360] C.costatus_neiba_102925_Los_Pinos ....C...... ------[360] C.costatus_neiba_161498_Cacique_Enriquillo ....C...... C...... T...... T...... [360] C._costatus_neiba_194362_Apolinar_Pelodroma ....C...... ------[360] C.costatus_neiba_194363_Apolinar_Pelodroma ....C...... ------[360] C.costatus_neiba_266321_Rosa_de_La_Pierda ....C...... C...... T...... T...... [360] C.costatus_neiba_266683_Rosa_de_La_Pierda ....C...... C...... T...... T...... [360] C.costatus_oreistes_104408_Jacmel ....C...... C.....G ...T...... [360] C.costatus_oreistes_192480_Puerto_Escondido ....C...... C.....G ...T...... [360] C.sepsoides_102369_Sabana_de_La_Mar ....C...... C...... G.. C.... [360] C.sepsoides_191850_Pestel ....C...... ------[360] C.sepsoides_191913_Marche_Leon ....C...... ------[360] C.sepsoides_192469_La_Zanga ....C...... ------[360] C.sepsoides_193201_Tenares ....C...... ------[360] C.sepsoides_266748_Jacmel ....C...... ------[360] C.sepsoides_266749_Jacmel ....C...... ------[360] C.stenurus_stenurus_103900_Rampe_des_Lions_Boios_Sec ....C...... ------[360] C.stenurus_stenurus_103901_Rampe_des_Lions_Boios_Sec ....C...... ------[360] C.stenurus_stenurus_103902_Rampe_des_Lions_Boios_Sec ....C...... ------[360] C.stenurus_stenurus_267494_lle_a_Vache ....C...... ------[360] C.haetiana_surdus_104418_Gros_Cheval ....C...... ------[360] C.haetiana_surdus_104419_Gros_Cheval ....C...... ------[360] C.haetiana_surdus_192486_Puerto_Escondido ....C...... ------[360] C.haetiana_surdus_192497_Puerto_Escondido ....C...... ------[360]

139

C.stenurus_rugosus_101247_Sabana_de_La_Mar ....C...... ------[360] C.stenurus_rugosus_101248_Sabana_de_La_Mar ....C...... ------[360] C.stenurus_rugosus_102371_El_Valle ....C...... G ...T...... G.. A.... [360] C.stenurus_rugosus_103075_Las_Galeras ....C...... G ...T...... G.. A.... [360] C.stenurus_rugosus_103083_Nagua ....C...... G ...T...... G.. A.... [360] C.stenurus_rugosus_103084_Nagua ....C...... ------[360] C.stenurus_rugosus_193191_San_Francisco_de_Macoris ....C...... ------[360] C.stenurus_rugosus_193192_San_Francisco_de_Macoris ....C...... ------[360] C.stenurus_rugosus_193197_Tenares ....C...... ------[360] C.stenurus_rugosus_193198_Tenares ....C...... ------[360] C.stenurus_rugosus_193216_Tenares ....C...... ------[360] C.stenurus_rugosus_193217_Tenares ....C...... ------[360] C.warreni_172914_Hispaniola ....C...... T...... [360] C.warreni_194521_Puerto_Plata ....C...... T...... [360] C.stenurus_weinlandi_102712_Canoa ....C...... ------[360] C.stenurus_weinlandi_103607_Soliette ....C...... ------[360] C.stenurus_weinlandi_191591_Thomaseau ....C...... ------[360] C.stenurus_weinlandi_191622_Thomaseau ....C...... G ...T...... G.. A.... [360] C.stenurus_weinlandi_192405_Thomaseau ....C...... G ...T...... G.. A.... [360] C.stenurus_weinlandi_266286_El_Azul ....C...... G ...T...... G.. A.... [360] C.stenurus_weinlandi_266682_La_Zurza ....C...... G ...T...... G.. A.... [360] C.stenurus_weinlandi_266684_La_Zurza ....C...... ------[360] C.stenurus_weinlandi_266685_La_Zurza ....C...... ------[360] D.bilobatus_194940_Costa_Rica .A..C...... ------[360]

140

D.nigropunctatus_191015_San_Luis_de_Potosi ....C...... G...... CACC...... A.A...... A.. [360] D.nigropunctatus_191319_Los_Calderos ....C...... G...... CACC...... A.A...... A.. [360] D.nigropunctatus_191320_Los_Calderos ....C...... G...... CACC...... A.A...... A.. [360] D.nigropunctatus_191542_San_Vincente ....C...... A...... TACC...... A.A..... C.A.. [360] D.garridoi_193507_El_Manguito ....C...... ------[360] D.pleii_191574_Rio_Abajo .A..C...... A...... CACC..G. ..A.A..... A.A.. [360] C.barbouri_161120_Quick_Step ....C...... [360] C.barbouri_190678_Tejunde ....C...... [360] C.crusculus_101571_Ewarton ....C...... [360] C.crusculus_101572_Content ....C...G...... [360] C.crusculus_172442_Duncans ....C...... [360] C.crusculus_101554_Town_Head ....C...... ------[360] C.crusculus_103448_Knoxwood ....C...... G ...T...... G.....T...... [360] C.crusculus_103449_Knowxwood ....C...... ------[360] C.crusculus_103460_Jackson_Bay ....C...... [360] C.crusculus_103586_Jackson_Bay ....C...... ------[360] C.crusculus_161123_Duncans ....C...... ------[360] C.crusculus_161124_Salt_Gut ....C...... [360] C.crusculus_171439_Oracabessa ....C...... ------[360] C.crusculus_171601_Port_Maria ....C...... ------[360] C.crusculus_171603_Port_Maria ....C...... ------[360] C.crusculus_171604_Port_Maria ....C...... ------[360] C.crusculus_171605_Port_Maria ....C...... ------[360] C.crusculus_171606_Oracabessa ....C...... ------[360]

141

C.crusculus_171607_Braeton ....C...... G...... G...... [360] C.crusculus_172438_Oracabessa ....C...... ------[360] C.crusculus_172441_Old_Hope ....C...... ------[360] C.crusculus_172465_Section .T..C...... [360] C.duquesneyi_267952_Hellshire_Hills ....C...... [360] C.hewardii_267097_Mandeville ....C...... G. .G...... [360] C.crusculus_101573_Hanover_Content ....C...... ------[360] C.crusculus_101615_Oracabessa ....C...... ------[360] C.crusculus_172439_Duncans ....C...... ------[360] C.crusculus_172440_Old_Hope ....C...... ------[360] C.crusculus_190695_Duncans ....C...... ------[360] C.sepsoides_193116_Majagual ....C...... ------[360] C.sepsoides_193117_Majagual ....C...... ------[360] C.sepsoides_193118_Majagual ....C...... ------[360] C.sepsoides_193202_Tenares ....C...... ------[360] C.sepsoides_193203_Tenares ....C...... ------[360] C.sepsoides_193204_Tenares ....C...... ------[360] C.sepsoides_193040_Cotui ....C...... ------[360] C.sepsoides_193041_Cotui ....C...... ------[360] C.sepsoides_193042_Cotui ....C...... ------[360] C.sepsoides_191851_Pestel ....C...... ------[360] C.sepsoides_191914_Marche_Leon ....C...... ------[360] C.sepsoides_191915_Marche_Leon ....C...... ------[360] C.sepsoides_192313_Baraderes ....C...... ------[360]

142

D.pleii_191575_Rio_Abajo .A..C...... ------[360] D.pleii_191576_Rio_Abajo .A..C...... ------[360] O.apodus_194938 ...... G...... TA....G. ..T.A...T. ..A.. [360] C.agasepsoides_160191_Canoa CCTCCCTAAT TATTGTAACC TGACAAAAAC TAGCACCAGC ATCCT [405] C.agasepsoides_194829_Bucan_Detwi ------[405] C.agasepsoides_194830_Bucan_Detwi ------[405] C.curtissi_aporus_102609_La_Descubierta ------[405] C.curtissi_aporus_102610_La_Descubierta ...... C.....T...... A. ...TC [405] C.curtissi_aporus_102611_La_Descubierta ------[405] C.curtissi_aporus_102706_Barahona ------[405] C.curtissi_aporus_102707_Juancho ------[405] C.curtissi_aporus_102709_Juancho ------[405] C.curtissi_aporus_102716_Barahona ------[405] C.curtissi_aporus_102915_Los_Arroyos ------[405] C.curtissi_aporus_102930_Barahona ...... C.....T...... A. ...TC [405] C.curtissi_aporus_194831_Bucan_Detwi ...... C.....T...... A. ....C [405] C.curtissi_aporus_194832_Bucan_Detwi ------[405] C.curtissi_aporus_194833_Bucan_Detwi ------[405] C.curtissi_aporus_194838_Bucan_Detwi ...... C.....T...... A. ....C [405] C.badius_194964_Navassa_Island .....T...... CC.C...... T..AC [405] C.costatus_costatus_103085_Castillion ------[405] C.costatus_costatus_104113_Castillion .....T.... C.....T...... A. ...TC [405] C.costatus_costatus_192054_Abricots .....T.... C.....T...... A. ...TC [405] C.costatus_costatus_192260_Beaumont ------[405]

143

C.cyanochloris_194930_Costa_Rica .AGTA..... C...A.C...... AT .A.AC [405] C.curtissi_curtissi_194503_La_Descubierta ...... C.....T...... A. ...TC [405] C.darlingtoni_161686_Cacique_Enriquillo .....T...... C...... C [405] C.darlingtoni_161687_Cacique_Enriquillo .....T...... C...... C [405] C.darlingtoni_161688_Cacique_Enriquillo .....T...... C...... C [405] C.curtissi_diastatus_194553_Mole_St._Nicolas ...... C.....C...... A. ...TC [405] C.curtissi_diastatus_194580_Mole_St._Nicolas ------[405] C.enneagrammus_191044_Mexico .AA.A..... C.....G...... AT .G.AC [405] C.maculatus_266550_Cayman_Brac_West ------[405] C.maculatus_266551_Cayman_Brac_West ------[405] C.maculatus_266552_Cayman_Brac_West ------[405] C.maculatus_266553_Cayman_Brac_West ------[405] C.maculatus_266527_Cayman_Brac_West .....T.... C..CC.C...... T ...A. [405] C.marcanoi_193279_Valle_de_Bao ...... C.....T...... A. ...TC [405] C.marcanoi_193280_Valle_de_Bao ------[405] C.marcanoi_193286_Valle_de_Bao ...... C.....T...... A. ...TC [405] C.marcanoi_193309_Valle_de_Bao ------[405] C.marcanoi_193310_Valle_de_Bao ------[405] C.haetiana_mylicus_102546_Cabral .....T...... A.T...... T ...... [405] C.haetiana_mylicus_102547_Cabral ------[405] C.haetiana_mylicus_102550_Cabral ------[405] C.haetiana_mylicus_160201_Tejunde ------[405] C.costatus_neiba_102922_La_Descubierta ...... C.....C...... TC [405] C.costatus_neiba_102923_Los_Pinos ------[405]

144

C.costatus_neiba_102924_Los_Pinos ------[405] C.costatus_neiba_102925_Los_Pinos ------[405] C.costatus_neiba_161498_Cacique_Enriquillo .....A.... C.....T...... G...A. ...TC [405] C._costatus_neiba_194362_Apolinar_Pelodroma ------[405] C.costatus_neiba_194363_Apolinar_Pelodroma ------[405] C.costatus_neiba_266321_Rosa_de_La_Pierda .....A.... C.....T...... G...A. ...TC [405] C.costatus_neiba_266683_Rosa_de_La_Pierda .....A.... C.....T...... G...A. ...TC [405] C.costatus_oreistes_104408_Jacmel .....T.... C.....T...... A. ...TC [405] C.costatus_oreistes_192480_Puerto_Escondido .....T.... C.....T...... A. ...TC [405] C.sepsoides_102369_Sabana_de_La_Mar ....TT...... T ...... A. .A..C [405] C.sepsoides_191850_Pestel ------[405] C.sepsoides_191913_Marche_Leon ------[405] C.sepsoides_192469_La_Zanga ------[405] C.sepsoides_193201_Tenares ------[405] C.sepsoides_266748_Jacmel ------[405] C.sepsoides_266749_Jacmel ------[405] C.stenurus_stenurus_103900_Rampe_des_Lions_Boios_Sec ------[405] C.stenurus_stenurus_103901_Rampe_des_Lions_Boios_Sec ------[405] C.stenurus_stenurus_103902_Rampe_des_Lions_Boios_Sec ------[405] C.stenurus_stenurus_267494_lle_a_Vache ------[405] C.haetiana_surdus_104418_Gros_Cheval ------[405] C.haetiana_surdus_104419_Gros_Cheval ------[405] C.haetiana_surdus_192486_Puerto_Escondido ------[405] C.haetiana_surdus_192497_Puerto_Escondido ------[405]

145

C.stenurus_rugosus_101247_Sabana_de_La_Mar ------[405] C.stenurus_rugosus_101248_Sabana_de_La_Mar ------[405] C.stenurus_rugosus_102371_El_Valle .....T.... C..CC.C...... AC [405] C.stenurus_rugosus_103075_Las_Galeras .....T.... C..CC.C...... AC [405] C.stenurus_rugosus_103083_Nagua .....T.... C..CC.C...... AC [405] C.stenurus_rugosus_103084_Nagua ------[405] C.stenurus_rugosus_193191_San_Francisco_de_Macoris ------[405] C.stenurus_rugosus_193192_San_Francisco_de_Macoris ------[405] C.stenurus_rugosus_193197_Tenares ------[405] C.stenurus_rugosus_193198_Tenares ------[405] C.stenurus_rugosus_193216_Tenares ------[405] C.stenurus_rugosus_193217_Tenares ------[405] C.warreni_172914_Hispaniola .....A...... T ...... TC [405] C.warreni_194521_Puerto_Plata .....A...... T ...... TC [405] C.stenurus_weinlandi_102712_Canoa ------[405] C.stenurus_weinlandi_103607_Soliette ------[405] C.stenurus_weinlandi_191591_Thomaseau ------[405] C.stenurus_weinlandi_191622_Thomaseau .....T.... C..CC.T...... AC [405] C.stenurus_weinlandi_192405_Thomaseau .....T...... CC.T...... AC [405] C.stenurus_weinlandi_266286_El_Azul ..G..T.... C..CC.C...... AC [405] C.stenurus_weinlandi_266682_La_Zurza ..G..T.... C..CC.C...... AC [405] C.stenurus_weinlandi_266684_La_Zurza ------[405] C.stenurus_weinlandi_266685_La_Zurza ------[405] D.bilobatus_194940_Costa_Rica ------[405]

146

D.nigropunctatus_191015_San_Luis_de_Potosi ..A..T..G. C..CA.C..A ...... TAT .A..C [405] D.nigropunctatus_191319_Los_Calderos ..A..T.... C..CA.C..A ...... TAT .A..C [405] D.nigropunctatus_191320_Los_Calderos ..A..T.... C..CA.C..A ...... TAT .A..C [405] D.nigropunctatus_191542_San_Vincente ..A..T.... C..CA.C..A ...... TAT .A..C [405] D.garridoi_193507_El_Manguito ------[405] D.pleii_191574_Rio_Abajo ..A...... C...A.T...... T ...... TAT .A..C [405] C.barbouri_161120_Quick_Step ...... C... ..G...... C [405] C.barbouri_190678_Tejunde ...... C... ..G...... C [405] C.crusculus_101571_Ewarton .....A...... T...... G..C [405] C.crusculus_101572_Content .....A...... T...... G..C [405] C.crusculus_172442_Duncans ...... T...... C [405] C.crusculus_101554_Town_Head ------[405] C.crusculus_103448_Knoxwood ...... A.T...... T ...... G...C [405] C.crusculus_103449_Knowxwood ------[405] C.crusculus_103460_Jackson_Bay ...... T...... C [405] C.crusculus_103586_Jackson_Bay ------[405] C.crusculus_161123_Duncans ------[405] C.crusculus_161124_Salt_Gut ...... T...... C [405] C.crusculus_171439_Oracabessa ------[405] C.crusculus_171601_Port_Maria ------[405] C.crusculus_171603_Port_Maria ------[405] C.crusculus_171604_Port_Maria ------[405] C.crusculus_171605_Port_Maria ------[405] C.crusculus_171606_Oracabessa ------[405]

147

C.crusculus_171607_Braeton ...... A.C...... AC [405] C.crusculus_172438_Oracabessa ------[405] C.crusculus_172441_Old_Hope ------[405] C.crusculus_172465_Section .....A...... T...... C...C [405] C.duquesneyi_267952_Hellshire_Hills .T...... T...... TC [405] C.hewardii_267097_Mandeville .T...... T...... G...... C [405] C.crusculus_101573_Hanover_Content ------[405] C.crusculus_101615_Oracabessa ------[405] C.crusculus_172439_Duncans ------[405] C.crusculus_172440_Old_Hope ------[405] C.crusculus_190695_Duncans ------[405] C.sepsoides_193116_Majagual ------[405] C.sepsoides_193117_Majagual ------[405] C.sepsoides_193118_Majagual ------[405] C.sepsoides_193202_Tenares ------[405] C.sepsoides_193203_Tenares ------[405] C.sepsoides_193204_Tenares ------[405] C.sepsoides_193040_Cotui ------[405] C.sepsoides_193041_Cotui ------[405] C.sepsoides_193042_Cotui ------[405] C.sepsoides_191851_Pestel ------[405] C.sepsoides_191914_Marche_Leon ------[405] C.sepsoides_191915_Marche_Leon ------[405] C.sepsoides_192313_Baraderes ------[405]

148

D.pleii_191575_Rio_Abajo ------[405] D.pleii_191576_Rio_Abajo ------[405] O.apodus_194938 .TGGA...... CA.C..G ...... T .C..C...AT .A..C [405] C.agasepsoides_160191_Canoa TGCTATACAT GACATGAAAT CAAATTTCCC CTACAATTAT CACGC [450] C.agasepsoides_194829_Bucan_Detwi ------[450] C.agasepsoides_194830_Bucan_Detwi ------[450] C.curtissi_aporus_102609_La_Descubierta ------[450] C.curtissi_aporus_102610_La_Descubierta .A..T...C. A...... C..A. .A.....C.. ...C. [450] C.curtissi_aporus_102611_La_Descubierta ------[450] C.curtissi_aporus_102706_Barahona ------[450] C.curtissi_aporus_102707_Juancho ------[450] C.curtissi_aporus_102709_Juancho ------[450] C.curtissi_aporus_102716_Barahona ------[450] C.curtissi_aporus_102915_Los_Arroyos ------[450] C.curtissi_aporus_102930_Barahona .A..T...C...... C..A. .A.....C.. ...C. [450] C.curtissi_aporus_194831_Bucan_Detwi .A..T...C. A...... C..A. .A.....C.. ...C. [450] C.curtissi_aporus_194832_Bucan_Detwi ------[450] C.curtissi_aporus_194833_Bucan_Detwi ------[450] C.curtissi_aporus_194838_Bucan_Detwi .A..T...C. A...... C..A. .A.....C.. ...C. [450] C.badius_194964_Navassa_Island .A..G...C. A...... C..T. .C...... A..CA [450] C.costatus_costatus_103085_Castillion ------[450] C.costatus_costatus_104113_Castillion .A...... C. A...... C..A. .A...G.C.. ...C. [450] C.costatus_costatus_192054_Abricots .A...... C. A...... C..A. .A...G.C.. ...C. [450] C.costatus_costatus_192260_Beaumont ------[450]

149

C.cyanochloris_194930_Costa_Rica .C..C...T. A..C.....C .....C..TG ..C...CC.. .T.AA [450] C.curtissi_curtissi_194503_La_Descubierta .A..T...C. A...... C..A. .A.....C.. T..CT [450] C.darlingtoni_161686_Cacique_Enriquillo .A..G...C. A...... C .....C..T. ...T..CC.. ...C. [450] C.darlingtoni_161687_Cacique_Enriquillo .A..G...C. A...... C .....C..T. ...T..CC.. ...C. [450] C.darlingtoni_161688_Cacique_Enriquillo .A..G...C. A...... C .....C..T. ...TG.CC.. ...C. [450] C.curtissi_diastatus_194553_Mole_St._Nicolas .A..T...C. A...... C..A. .A.....C.. ...CT [450] C.curtissi_diastatus_194580_Mole_St._Nicolas ------[450] C.enneagrammus_191044_Mexico .T..C.T.C. A...... C ....C...AA .ACTT.C... TT.AA [450] C.maculatus_266550_Cayman_Brac_West ------[450] C.maculatus_266551_Cayman_Brac_West ------[450] C.maculatus_266552_Cayman_Brac_West ------[450] C.maculatus_266553_Cayman_Brac_West ------[450] C.maculatus_266527_Cayman_Brac_West .A..T...C. A...... C.... .C...... A..C. [450] C.marcanoi_193279_Valle_de_Bao .A..T...C. A...... C..A. .A.....C.. ...CT [450] C.marcanoi_193280_Valle_de_Bao ------[450] C.marcanoi_193286_Valle_de_Bao .A..T...C. A...... C..A. .A.....C.. ...CT [450] C.marcanoi_193309_Valle_de_Bao ------[450] C.marcanoi_193310_Valle_de_Bao ------[450] C.haetiana_mylicus_102546_Cabral .A...... C. A...... C .....C.... .C.....C.. T..T. [450] C.haetiana_mylicus_102547_Cabral ------[450] C.haetiana_mylicus_102550_Cabral ------[450] C.haetiana_mylicus_160201_Tejunde ------[450] C.costatus_neiba_102922_La_Descubierta .A..T...C. A...... C..A. .A...G.C.. ...CT [450] C.costatus_neiba_102923_Los_Pinos ------[450]

150

C.costatus_neiba_102924_Los_Pinos ------[450] C.costatus_neiba_102925_Los_Pinos ------[450] C.costatus_neiba_161498_Cacique_Enriquillo .A..T...C. A...... C..A. .A...G.C.. ...CT [450] C._costatus_neiba_194362_Apolinar_Pelodroma ------[450] C.costatus_neiba_194363_Apolinar_Pelodroma ------[450] C.costatus_neiba_266321_Rosa_de_La_Pierda .A..T...C. A...... C..A. .A...G.C.. ...CT [450] C.costatus_neiba_266683_Rosa_de_La_Pierda .A..T...C. A...... C..A. .A...G.C.. ...CT [450] C.costatus_oreistes_104408_Jacmel .A...... C. A...... C .....C..A. .A...G.C.. ...C. [450] C.costatus_oreistes_192480_Puerto_Escondido .A...... C. A...... C .....C..A. .A...G.C.. ...C. [450] C.sepsoides_102369_Sabana_de_La_Mar .C...... C. A...... C.....C.. ...CA [450] C.sepsoides_191850_Pestel ------[450] C.sepsoides_191913_Marche_Leon ------[450] C.sepsoides_192469_La_Zanga ------[450] C.sepsoides_193201_Tenares ------[450] C.sepsoides_266748_Jacmel ------[450] C.sepsoides_266749_Jacmel ------[450] C.stenurus_stenurus_103900_Rampe_des_Lions_Boios_Sec ------[450] C.stenurus_stenurus_103901_Rampe_des_Lions_Boios_Sec ------[450] C.stenurus_stenurus_103902_Rampe_des_Lions_Boios_Sec ------[450] C.stenurus_stenurus_267494_lle_a_Vache ------[450] C.haetiana_surdus_104418_Gros_Cheval ------[450] C.haetiana_surdus_104419_Gros_Cheval ------[450] C.haetiana_surdus_192486_Puerto_Escondido ------[450] C.haetiana_surdus_192497_Puerto_Escondido ------[450]

151

C.stenurus_rugosus_101247_Sabana_de_La_Mar ------[450] C.stenurus_rugosus_101248_Sabana_de_La_Mar ------[450] C.stenurus_rugosus_102371_El_Valle .A..C...C. A...... C.... .C...G.... A..CA [450] C.stenurus_rugosus_103075_Las_Galeras .A..C...C. A...... C.... .C...G.... A..CA [450] C.stenurus_rugosus_103083_Nagua .A..C...C. A...... C.... .C...G.... A..CA [450] C.stenurus_rugosus_103084_Nagua ------[450] C.stenurus_rugosus_193191_San_Francisco_de_Macoris ------[450] C.stenurus_rugosus_193192_San_Francisco_de_Macoris ------[450] C.stenurus_rugosus_193197_Tenares ------[450] C.stenurus_rugosus_193198_Tenares ------[450] C.stenurus_rugosus_193216_Tenares ------[450] C.stenurus_rugosus_193217_Tenares ------[450] C.warreni_172914_Hispaniola .A...... C. A...... C .....C.... .C...G.C.. A..C. [450] C.warreni_194521_Puerto_Plata .A...... C. A...... C .....C.... .C...G.C.. A..C. [450] C.stenurus_weinlandi_102712_Canoa ------[450] C.stenurus_weinlandi_103607_Soliette ------[450] C.stenurus_weinlandi_191591_Thomaseau ------[450] C.stenurus_weinlandi_191622_Thomaseau .A..C...T. A...... C.... .C...G.... A..C. [450] C.stenurus_weinlandi_192405_Thomaseau .A..C...C. A...... C.... .C...G.... A..C. [450] C.stenurus_weinlandi_266286_El_Azul .A..C...C. A...... C.... .C...G.... A..C. [450] C.stenurus_weinlandi_266682_La_Zurza .A..C...C. A...... C.... .C...G.... A..C. [450] C.stenurus_weinlandi_266684_La_Zurza ------[450] C.stenurus_weinlandi_266685_La_Zurza ------[450] D.bilobatus_194940_Costa_Rica ------[450]

152

D.nigropunctatus_191015_San_Luis_de_Potosi .C...... A...... C ....CC..AT .A.T..CC.. A..AA [450] D.nigropunctatus_191319_Los_Calderos .C...... A...... CC..AT .A.T..CC.. G..AA [450] D.nigropunctatus_191320_Los_Calderos .C...... A...... CC..AT .A.T..CC.. G..AA [450] D.nigropunctatus_191542_San_Vincente .C...... A...... CC..AT .A....CC.. A..AA [450] D.garridoi_193507_El_Manguito ------[450] D.pleii_191574_Rio_Abajo .T...... C. C...... G.C .....C..A. .A.....C.. A..CA [450] C.barbouri_161120_Quick_Step .A...... T...... A. .C...G.C.. ...C. [450] C.barbouri_190678_Tejunde .A...... T...... A. .C...G.C.. ...C. [450] C.crusculus_101571_Ewarton ...... T. A...... C..A. .C...G.C.. ...C. [450] C.crusculus_101572_Content ...... T. A...... C..A. .C...G.C.. ...C. [450] C.crusculus_172442_Duncans ...... T. A...... C..A. .C...G.C.. ...C. [450] C.crusculus_101554_Town_Head ------[450] C.crusculus_103448_Knoxwood .A...... C. A...... C..A. .C...G.C.. ...CT [450] C.crusculus_103449_Knowxwood ------[450] C.crusculus_103460_Jackson_Bay ...... T. A...... C..A. .C...G.C.. ...C. [450] C.crusculus_103586_Jackson_Bay ------[450] C.crusculus_161123_Duncans ------[450] C.crusculus_161124_Salt_Gut ...... T. A...... C..A. .C...G.C.. ...C. [450] C.crusculus_171439_Oracabessa ------[450] C.crusculus_171601_Port_Maria ------[450] C.crusculus_171603_Port_Maria ------[450] C.crusculus_171604_Port_Maria ------[450] C.crusculus_171605_Port_Maria ------[450] C.crusculus_171606_Oracabessa ------[450]

153

C.crusculus_171607_Braeton .A...... T. A...... C .....C..A. .C...G.C.. ...C. [450] C.crusculus_172438_Oracabessa ------[450] C.crusculus_172441_Old_Hope ------[450] C.crusculus_172465_Section .AT.....T. A...... C .....C..A. .CC..G.C.. ...C. [450] C.duquesneyi_267952_Hellshire_Hills .A...... T. A...... C..A. .C...G.C.. ...C. [450] C.hewardii_267097_Mandeville .A...... T. A...... C..A. .C...G.C.. ...C. [450] C.crusculus_101573_Hanover_Content ------[450] C.crusculus_101615_Oracabessa ------[450] C.crusculus_172439_Duncans ------[450] C.crusculus_172440_Old_Hope ------[450] C.crusculus_190695_Duncans ------[450] C.sepsoides_193116_Majagual ------[450] C.sepsoides_193117_Majagual ------[450] C.sepsoides_193118_Majagual ------[450] C.sepsoides_193202_Tenares ------[450] C.sepsoides_193203_Tenares ------[450] C.sepsoides_193204_Tenares ------[450] C.sepsoides_193040_Cotui ------[450] C.sepsoides_193041_Cotui ------[450] C.sepsoides_193042_Cotui ------[450] C.sepsoides_191851_Pestel ------[450] C.sepsoides_191914_Marche_Leon ------[450] C.sepsoides_191915_Marche_Leon ------[450] C.sepsoides_192313_Baraderes ------[450]

154

D.pleii_191575_Rio_Abajo ------[450] D.pleii_191576_Rio_Abajo ------[450] O.apodus_194938 ....CCTTC. T...... C ....CC..TA TGCT..CCC. ...AG [450] C.agasepsoides_160191_Canoa TAATGGGTAC ACTTTCAGTA ATTGTAGGAG GACTAGGAGG ACTTA [495] C.agasepsoides_194829_Bucan_Detwi ------[495] C.agasepsoides_194830_Bucan_Detwi ------[495] C.curtissi_aporus_102609_La_Descubierta ------[495] C.curtissi_aporus_102610_La_Descubierta ....A..CG. ...A...... C...... G.. ...C. [495] C.curtissi_aporus_102611_La_Descubierta ------[495] C.curtissi_aporus_102706_Barahona ------[495] C.curtissi_aporus_102707_Juancho ------[495] C.curtissi_aporus_102709_Juancho ------[495] C.curtissi_aporus_102716_Barahona ------[495] C.curtissi_aporus_102915_Los_Arroyos ------[495] C.curtissi_aporus_102930_Barahona ....A..CG. ...A...... C...... G.. ...C. [495] C.curtissi_aporus_194831_Bucan_Detwi ....A..CG. ...A...... C...... G.. ...C. [495] C.curtissi_aporus_194832_Bucan_Detwi ------[495] C.curtissi_aporus_194833_Bucan_Detwi ------[495] C.curtissi_aporus_194838_Bucan_Detwi ....A..CG. ...A...... C...... G.. ...C. [495] C.badius_194964_Navassa_Island ....A..AG. ...A...... G...... G. ..T...... A. [495] C.costatus_costatus_103085_Castillion ------[495] C.costatus_costatus_104113_Castillion .G..A..CG. ...G...... G..C. ..T....G.. ...C. [495] C.costatus_costatus_192054_Abricots .G..A..CG. ...G...... G..C. ..T....G.. ...C. [495] C.costatus_costatus_192260_Beaumont ------[495]

155

C.cyanochloris_194930_Costa_Rica CT..A..G.T ...A..C... G.A...... C..G..C.. ...C. [495] C.curtissi_curtissi_194503_La_Descubierta ...... CG. ...A...... C...... C. [495] C.darlingtoni_161686_Cacique_Enriquillo .G...... G. ...A..G... G...... T. .C...... C. [495] C.darlingtoni_161687_Cacique_Enriquillo .G...... G. ...A..G... G...... T. .C...... C. [495] C.darlingtoni_161688_Cacique_Enriquillo .G...... G. ...A..G... G...... T. .C...... C. [495] C.curtissi_diastatus_194553_Mole_St._Nicolas ....A..CG. ...A...... C...... G.. ...C. [495] C.curtissi_diastatus_194580_Mole_St._Nicolas ------[495] C.enneagrammus_191044_Mexico C...A..GG. C..C..C..T C.A..T...... T.. ...C. [495] C.maculatus_266550_Cayman_Brac_West ------[495] C.maculatus_266551_Cayman_Brac_West ------[495] C.maculatus_266552_Cayman_Brac_West ------[495] C.maculatus_266553_Cayman_Brac_West ------[495] C.maculatus_266527_Cayman_Brac_West ....A..AG. ...C...... G...... T...... [495] C.marcanoi_193279_Valle_de_Bao ...... G. ...A...... C. ..T...... C. [495] C.marcanoi_193280_Valle_de_Bao ------[495] C.marcanoi_193286_Valle_de_Bao ...... G. ...A...... C. ..T...... C. [495] C.marcanoi_193309_Valle_de_Bao ------[495] C.marcanoi_193310_Valle_de_Bao ------[495] C.haetiana_mylicus_102546_Cabral .G..A..C.. ...A...... [495] C.haetiana_mylicus_102547_Cabral ------[495] C.haetiana_mylicus_102550_Cabral ------[495] C.haetiana_mylicus_160201_Tejunde ------[495] C.costatus_neiba_102922_La_Descubierta ....A..CG. ...A.....G ...... C. ..T....G.. ...C. [495] C.costatus_neiba_102923_Los_Pinos ------[495]

156

C.costatus_neiba_102924_Los_Pinos ------[495] C.costatus_neiba_102925_Los_Pinos ------[495] C.costatus_neiba_161498_Cacique_Enriquillo ....A..CG. ...A...... G..T. ..T....G.. ...C. [495] C._costatus_neiba_194362_Apolinar_Pelodroma ------[495] C.costatus_neiba_194363_Apolinar_Pelodroma ------[495] C.costatus_neiba_266321_Rosa_de_La_Pierda ....A..CG. ...A...... G..T. ..T....G.. ...C. [495] C.costatus_neiba_266683_Rosa_de_La_Pierda ....A..CG. ...A...... G..T. ..T....G.. ...C. [495] C.costatus_oreistes_104408_Jacmel .G..A..CG. ...A...... C. ..T....G.. G..C. [495] C.costatus_oreistes_192480_Puerto_Escondido .G..A...G. ...A...... C. ..T....G.. ...C. [495] C.sepsoides_102369_Sabana_de_La_Mar ....A..CG. ...A...... T...... [495] C.sepsoides_191850_Pestel ------[495] C.sepsoides_191913_Marche_Leon ------[495] C.sepsoides_192469_La_Zanga ------[495] C.sepsoides_193201_Tenares ------[495] C.sepsoides_266748_Jacmel ------[495] C.sepsoides_266749_Jacmel ------[495] C.stenurus_stenurus_103900_Rampe_des_Lions_Boios_Sec ------[495] C.stenurus_stenurus_103901_Rampe_des_Lions_Boios_Sec ------[495] C.stenurus_stenurus_103902_Rampe_des_Lions_Boios_Sec ------[495] C.stenurus_stenurus_267494_lle_a_Vache ------[495] C.haetiana_surdus_104418_Gros_Cheval ------[495] C.haetiana_surdus_104419_Gros_Cheval ------[495] C.haetiana_surdus_192486_Puerto_Escondido ------[495] C.haetiana_surdus_192497_Puerto_Escondido ------[495]

157

C.stenurus_rugosus_101247_Sabana_de_La_Mar ------[495] C.stenurus_rugosus_101248_Sabana_de_La_Mar ------[495] C.stenurus_rugosus_102371_El_Valle ....A..AG. ...C...... C. [495] C.stenurus_rugosus_103075_Las_Galeras ....A..AG. ...C...... C. [495] C.stenurus_rugosus_103083_Nagua ....A..AG. ...C...... C. [495] C.stenurus_rugosus_103084_Nagua ------[495] C.stenurus_rugosus_193191_San_Francisco_de_Macoris ------[495] C.stenurus_rugosus_193192_San_Francisco_de_Macoris ------[495] C.stenurus_rugosus_193197_Tenares ------[495] C.stenurus_rugosus_193198_Tenares ------[495] C.stenurus_rugosus_193216_Tenares ------[495] C.stenurus_rugosus_193217_Tenares ------[495] C.warreni_172914_Hispaniola ....A..C.. ...A.....G ..C...... C...... A... [495] C.warreni_194521_Puerto_Plata ....A..C.. ...A.....G ..C...... C...... A... [495] C.stenurus_weinlandi_102712_Canoa ------[495] C.stenurus_weinlandi_103607_Soliette ------[495] C.stenurus_weinlandi_191591_Thomaseau ------[495] C.stenurus_weinlandi_191622_Thomaseau ....A..AG. ...C...... C. [495] C.stenurus_weinlandi_192405_Thomaseau ....A..AG. ...C...... C. [495] C.stenurus_weinlandi_266286_El_Azul ....A..AG. ...C...... C...... G..C. [495] C.stenurus_weinlandi_266682_La_Zurza ....A..AG. ...C...... C...... G..C. [495] C.stenurus_weinlandi_266684_La_Zurza ------[495] C.stenurus_weinlandi_266685_La_Zurza ------[495] D.bilobatus_194940_Costa_Rica ------[495]

158

D.nigropunctatus_191015_San_Luis_de_Potosi .T..A..CG. ...G..T..C C.AA.T...... T...... A. [495] D.nigropunctatus_191319_Los_Calderos .T..A..CG. ...G..C..C C.AA.T...... T...... A. [495] D.nigropunctatus_191320_Los_Calderos .T..A..CG. ...G..C..C C.AA.T...... T...... A. [495] D.nigropunctatus_191542_San_Vincente .T..A..CG. ...G.....C C.AA.T..G. ....T...... A. [495] D.garridoi_193507_El_Manguito ------[495] D.pleii_191574_Rio_Abajo CC.....C.T ...A..C..C C.AA.C...... G.. ...G. [495] C.barbouri_161120_Quick_Step ....A..CG. ...A...... C. [495] C.barbouri_190678_Tejunde ....A..CG. ...A...... C. [495] C.crusculus_101571_Ewarton ....A..CG. ...A...... A. [495] C.crusculus_101572_Content ....A..CG. ...A...... A. [495] C.crusculus_172442_Duncans ....A..GG. ...A...... A. [495] C.crusculus_101554_Town_Head ------[495] C.crusculus_103448_Knoxwood ....A..CG. ...A...... G..C. [495] C.crusculus_103449_Knowxwood ------[495] C.crusculus_103460_Jackson_Bay ....A..GG. ...A...... G...... G.. ...A. [495] C.crusculus_103586_Jackson_Bay ------[495] C.crusculus_161123_Duncans ------[495] C.crusculus_161124_Salt_Gut ....A..GG. ...A...... A. [495] C.crusculus_171439_Oracabessa ------[495] C.crusculus_171601_Port_Maria ------[495] C.crusculus_171603_Port_Maria ------[495] C.crusculus_171604_Port_Maria ------[495] C.crusculus_171605_Port_Maria ------[495] C.crusculus_171606_Oracabessa ------[495]

159

C.crusculus_171607_Braeton ....A..CG. ...A...... C. [495] C.crusculus_172438_Oracabessa ------[495] C.crusculus_172441_Old_Hope ------[495] C.crusculus_172465_Section ....A...G. ...A...... G. ....G..... G..C. [495] C.duquesneyi_267952_Hellshire_Hills .T..A...G. ...A...... G...... G...... C. [495] C.hewardii_267097_Mandeville ....A..CG. ...A...... G..C. [495] C.crusculus_101573_Hanover_Content ------[495] C.crusculus_101615_Oracabessa ------[495] C.crusculus_172439_Duncans ------[495] C.crusculus_172440_Old_Hope ------[495] C.crusculus_190695_Duncans ------[495] C.sepsoides_193116_Majagual ------[495] C.sepsoides_193117_Majagual ------[495] C.sepsoides_193118_Majagual ------[495] C.sepsoides_193202_Tenares ------[495] C.sepsoides_193203_Tenares ------[495] C.sepsoides_193204_Tenares ------[495] C.sepsoides_193040_Cotui ------[495] C.sepsoides_193041_Cotui ------[495] C.sepsoides_193042_Cotui ------[495] C.sepsoides_191851_Pestel ------[495] C.sepsoides_191914_Marche_Leon ------[495] C.sepsoides_191915_Marche_Leon ------[495] C.sepsoides_192313_Baraderes ------[495]

160

D.pleii_191575_Rio_Abajo ------[495] D.pleii_191576_Rio_Abajo ------[495] O.apodus_194938 ...... AT. CT.A...... C..G..G. .G..G..G.. G..A. [495] C.agasepsoides_160191_Canoa ACCAAACACA GCTCCGAAAG ATTATCGCCT ACTCATCAAT CGCCC [540] C.agasepsoides_194829_Bucan_Detwi ------[540] C.agasepsoides_194830_Bucan_Detwi ------[540] C.curtissi_aporus_102609_La_Descubierta ------[540] C.curtissi_aporus_102610_La_Descubierta ...... T.. AA.G.....A ..C...... [540] C.curtissi_aporus_102611_La_Descubierta ------[540] C.curtissi_aporus_102706_Barahona ------[540] C.curtissi_aporus_102707_Juancho ------[540] C.curtissi_aporus_102709_Juancho ------[540] C.curtissi_aporus_102716_Barahona ------[540] C.curtissi_aporus_102915_Los_Arroyos ------[540] C.curtissi_aporus_102930_Barahona ...... T.. AA.G.....A ..C...... [540] C.curtissi_aporus_194831_Bucan_Detwi ...... T.. AA.G.....A ..C...... [540] C.curtissi_aporus_194832_Bucan_Detwi ------[540] C.curtissi_aporus_194833_Bucan_Detwi ------[540] C.curtissi_aporus_194838_Bucan_Detwi ...... T.. AA.G.....A ..C...... [540] C.badius_194964_Navassa_Island ...... T.. A..A..G..A ..C...... [540] C.costatus_costatus_103085_Castillion ------[540] C.costatus_costatus_104113_Castillion ...... T.. A..A.....A ..C..T...... C...... [540] C.costatus_costatus_192054_Abricots ...... T.. A..A.....A ..C..T...... C...... [540] C.costatus_costatus_192260_Beaumont ------[540]

161

C.cyanochloris_194930_Costa_Rica .T.....C.. A..A.....A ..C.....A...... A. [540] C.curtissi_curtissi_194503_La_Descubierta ...... T.. ...A.....A ..C.....T...... T.... [540] C.darlingtoni_161686_Cacique_Enriquillo ...... C...... A ..C..T...... C...... [540] C.darlingtoni_161687_Cacique_Enriquillo ...... C...... A ..C..T...... C...... [540] C.darlingtoni_161688_Cacique_Enriquillo ...... C...... A ..C..T...... C...... [540] C.curtissi_diastatus_194553_Mole_St._Nicolas ...... T.. A..A.....A ..C...... [540] C.curtissi_diastatus_194580_Mole_St._Nicolas ------[540] C.enneagrammus_191044_Mexico ...... G.. A...... A ..C..T..A. T...... T..A. [540] C.maculatus_266550_Cayman_Brac_West ------[540] C.maculatus_266551_Cayman_Brac_West ------[540] C.maculatus_266552_Cayman_Brac_West ------[540] C.maculatus_266553_Cayman_Brac_West ------[540] C.maculatus_266527_Cayman_Brac_West ...... T.. A..A..G..A ..C...... [540] C.marcanoi_193279_Valle_de_Bao ...... T.. A..G.....A ..C...... [540] C.marcanoi_193280_Valle_de_Bao ------[540] C.marcanoi_193286_Valle_de_Bao ...... T.. A..G.....A ..C...... [540] C.marcanoi_193309_Valle_de_Bao ------[540] C.marcanoi_193310_Valle_de_Bao ------[540] C.haetiana_mylicus_102546_Cabral ...... A..A...... C..T...... [540] C.haetiana_mylicus_102547_Cabral ------[540] C.haetiana_mylicus_102550_Cabral ------[540] C.haetiana_mylicus_160201_Tejunde ------[540] C.costatus_neiba_102922_La_Descubierta ...... C.. A..A.....A ..C...... [540] C.costatus_neiba_102923_Los_Pinos ------[540]

162

C.costatus_neiba_102924_Los_Pinos ------[540] C.costatus_neiba_102925_Los_Pinos ------[540] C.costatus_neiba_161498_Cacique_Enriquillo ...... T.. A..A.....A ..C...... A. [540] C._costatus_neiba_194362_Apolinar_Pelodroma ------[540] C.costatus_neiba_194363_Apolinar_Pelodroma ------[540] C.costatus_neiba_266321_Rosa_de_La_Pierda ...... T.. A..A.....A ..C...... A. [540] C.costatus_neiba_266683_Rosa_de_La_Pierda ...... T.. A..A.....A ..C...... A. [540] C.costatus_oreistes_104408_Jacmel ...... T.. A..A.....A ..C.....T...... [540] C.costatus_oreistes_192480_Puerto_Escondido ...... T.. ...A.....A ..C...... G. [540] C.sepsoides_102369_Sabana_de_La_Mar ...... A..A.....A ..C...... A. [540] C.sepsoides_191850_Pestel ------[540] C.sepsoides_191913_Marche_Leon ------[540] C.sepsoides_192469_La_Zanga ------[540] C.sepsoides_193201_Tenares ------[540] C.sepsoides_266748_Jacmel ------[540] C.sepsoides_266749_Jacmel ------[540] C.stenurus_stenurus_103900_Rampe_des_Lions_Boios_Sec ------[540] C.stenurus_stenurus_103901_Rampe_des_Lions_Boios_Sec ------[540] C.stenurus_stenurus_103902_Rampe_des_Lions_Boios_Sec ------[540] C.stenurus_stenurus_267494_lle_a_Vache ------[540] C.haetiana_surdus_104418_Gros_Cheval ------[540] C.haetiana_surdus_104419_Gros_Cheval ------[540] C.haetiana_surdus_192486_Puerto_Escondido ------[540] C.haetiana_surdus_192497_Puerto_Escondido ------[540]

163

C.stenurus_rugosus_101247_Sabana_de_La_Mar ------[540] C.stenurus_rugosus_101248_Sabana_de_La_Mar ------[540] C.stenurus_rugosus_102371_El_Valle ...... T.. A..A..G..A ..C...... [540] C.stenurus_rugosus_103075_Las_Galeras ...... T.. A..A..G..A ..C...... [540] C.stenurus_rugosus_103083_Nagua ...... T.. A..A..G..A ..C...... [540] C.stenurus_rugosus_103084_Nagua ------[540] C.stenurus_rugosus_193191_San_Francisco_de_Macoris ------[540] C.stenurus_rugosus_193192_San_Francisco_de_Macoris ------[540] C.stenurus_rugosus_193197_Tenares ------[540] C.stenurus_rugosus_193198_Tenares ------[540] C.stenurus_rugosus_193216_Tenares ------[540] C.stenurus_rugosus_193217_Tenares ------[540] C.warreni_172914_Hispaniola ...... A...... A ..C...... C...... [540] C.warreni_194521_Puerto_Plata ...... A...... A ..C...... C...... [540] C.stenurus_weinlandi_102712_Canoa ------[540] C.stenurus_weinlandi_103607_Soliette ------[540] C.stenurus_weinlandi_191591_Thomaseau ------[540] C.stenurus_weinlandi_191622_Thomaseau ...... T.. A..A..G..A ..C...... [540] C.stenurus_weinlandi_192405_Thomaseau ...... T.. A..A..G..A ..C...... [540] C.stenurus_weinlandi_266286_El_Azul ...... T.. A..A.....A ..C...... [540] C.stenurus_weinlandi_266682_La_Zurza ...... T.. A..A.....A ..C...... [540] C.stenurus_weinlandi_266684_La_Zurza ------[540] C.stenurus_weinlandi_266685_La_Zurza ------[540] D.bilobatus_194940_Costa_Rica ------[540]

164

D.nigropunctatus_191015_San_Luis_de_Potosi ...... G.....A G.A.....A. ....C...... A. [540] D.nigropunctatus_191319_Los_Calderos ...... A.....A G.A.....A. ....C...... A. [540] D.nigropunctatus_191320_Los_Calderos ...... A.....A G.A.....A. ....C...... A. [540] D.nigropunctatus_191542_San_Vincente ...... G.....A G.A.....A. ....C...... A. [540] D.garridoi_193507_El_Manguito ------[540] D.pleii_191574_Rio_Abajo ...... G.. .A.G.....A G.A..T..A...... T..A. [540] C.barbouri_161120_Quick_Step ...... T.. A..A.....A ..C...... C...... A. [540] C.barbouri_190678_Tejunde ...... T.. A..A.....A ..C...... C...... A. [540] C.crusculus_101571_Ewarton ...... G.. AT.A.....A ..C...... C...... [540] C.crusculus_101572_Content ...... G.. AT.A.....A ..C...... C...... [540] C.crusculus_172442_Duncans ...... G.. A..A.....A ..C...... C...... [540] C.crusculus_101554_Town_Head ------[540] C.crusculus_103448_Knoxwood ....G..C.. A..A...... C...... [540] C.crusculus_103449_Knowxwood ------[540] C.crusculus_103460_Jackson_Bay ...... G.. A..A.A...... C...... C...... [540] C.crusculus_103586_Jackson_Bay ------[540] C.crusculus_161123_Duncans ------[540] C.crusculus_161124_Salt_Gut ...... G.. A..A.....A ..C...... C...... [540] C.crusculus_171439_Oracabessa ------[540] C.crusculus_171601_Port_Maria ------[540] C.crusculus_171603_Port_Maria ------[540] C.crusculus_171604_Port_Maria ------[540] C.crusculus_171605_Port_Maria ------[540] C.crusculus_171606_Oracabessa ------[540]

165

C.crusculus_171607_Braeton ...... T.. A..A.....A ..C..T...... C..... T.... [540] C.crusculus_172438_Oracabessa ------[540] C.crusculus_172441_Old_Hope ------[540] C.crusculus_172465_Section ...... T.. A..A...... C...... C...... [540] C.duquesneyi_267952_Hellshire_Hills ...... T.. A..A.....A ..C...... C...... [540] C.hewardii_267097_Mandeville ...... T.. A..A.....A ..C...... C...... [540] C.crusculus_101573_Hanover_Content ------[540] C.crusculus_101615_Oracabessa ------[540] C.crusculus_172439_Duncans ------[540] C.crusculus_172440_Old_Hope ------[540] C.crusculus_190695_Duncans ------[540] C.sepsoides_193116_Majagual ------[540] C.sepsoides_193117_Majagual ------[540] C.sepsoides_193118_Majagual ------[540] C.sepsoides_193202_Tenares ------[540] C.sepsoides_193203_Tenares ------[540] C.sepsoides_193204_Tenares ------[540] C.sepsoides_193040_Cotui ------[540] C.sepsoides_193041_Cotui ------[540] C.sepsoides_193042_Cotui ------[540] C.sepsoides_191851_Pestel ------[540] C.sepsoides_191914_Marche_Leon ------[540] C.sepsoides_191915_Marche_Leon ------[540] C.sepsoides_192313_Baraderes ------[540]

166

D.pleii_191575_Rio_Abajo ------[540] D.pleii_191576_Rio_Abajo ------[540] O.apodus_194938 ....G...... A.....A ..C.....A. T...C..T...... [540] C.agasepsoides_160191_Canoa ACCTAGGATG AATAGCCACA ATCATTTCAA AATCCAACAA ACTAG [585] C.agasepsoides_194829_Bucan_Detwi ------[585] C.agasepsoides_194830_Bucan_Detwi ------[585] C.curtissi_aporus_102609_La_Descubierta ------[585] C.curtissi_aporus_102610_La_Descubierta ...... T..A..T...... [585] C.curtissi_aporus_102611_La_Descubierta ------[585] C.curtissi_aporus_102706_Barahona ------[585] C.curtissi_aporus_102707_Juancho ------[585] C.curtissi_aporus_102709_Juancho ------[585] C.curtissi_aporus_102716_Barahona ------[585] C.curtissi_aporus_102915_Los_Arroyos ------[585] C.curtissi_aporus_102930_Barahona ...... G...... T..A..T...... [585] C.curtissi_aporus_194831_Bucan_Detwi ...... G...... T..A..T...... [585] C.curtissi_aporus_194832_Bucan_Detwi ------[585] C.curtissi_aporus_194833_Bucan_Detwi ------[585] C.curtissi_aporus_194838_Bucan_Detwi ...... G...... T..A..T...... [585] C.badius_194964_Navassa_Island ...... T..C..T. ....T...... [585] C.costatus_costatus_103085_Castillion ------[585] C.costatus_costatus_104113_Castillion ...... G...... T..A...... T...... [585] C.costatus_costatus_192054_Abricots ...... G...... T..A.... .G..T..T...... [585] C.costatus_costatus_192260_Beaumont ------[585]

167

C.cyanochloris_194930_Costa_Rica ..T...... T...... T. .G..AC.A...... [585] C.curtissi_curtissi_194503_La_Descubierta ...... TT.A...... [585] C.darlingtoni_161686_Cacique_Enriquillo ...... C...... [585] C.darlingtoni_161687_Cacique_Enriquillo ...... C...... T...... [585] C.darlingtoni_161688_Cacique_Enriquillo ...... C...... [585] C.curtissi_diastatus_194553_Mole_St._Nicolas ...... G.. ...G...... T..A...... [585] C.curtissi_diastatus_194580_Mole_St._Nicolas ------[585] C.enneagrammus_191044_Mexico ..T...... G.....T..C ...... C...... C...... T. [585] C.maculatus_266550_Cayman_Brac_West ------[585] C.maculatus_266551_Cayman_Brac_West ------[585] C.maculatus_266552_Cayman_Brac_West ------[585] C.maculatus_266553_Cayman_Brac_West ------[585] C.maculatus_266527_Cayman_Brac_West .T...... G...... C..T. .G...... [585] C.marcanoi_193279_Valle_de_Bao ...... G. ...G...... T..A...... [585] C.marcanoi_193280_Valle_de_Bao ------[585] C.marcanoi_193286_Valle_de_Bao ...... G...... T..A...... [585] C.marcanoi_193309_Valle_de_Bao ------[585] C.marcanoi_193310_Valle_de_Bao ------[585] C.haetiana_mylicus_102546_Cabral ....T...... C...... T...... [585] C.haetiana_mylicus_102547_Cabral ------[585] C.haetiana_mylicus_102550_Cabral ------[585] C.haetiana_mylicus_160201_Tejunde ------[585] C.costatus_neiba_102922_La_Descubierta ...... G...... T..A...... [585] C.costatus_neiba_102923_Los_Pinos ------[585]

168

C.costatus_neiba_102924_Los_Pinos ------[585] C.costatus_neiba_102925_Los_Pinos ------[585] C.costatus_neiba_161498_Cacique_Enriquillo ...... G...... T..A...... [585] C._costatus_neiba_194362_Apolinar_Pelodroma ------[585] C.costatus_neiba_194363_Apolinar_Pelodroma ------[585] C.costatus_neiba_266321_Rosa_de_La_Pierda ...... G...... T..A...... [585] C.costatus_neiba_266683_Rosa_de_La_Pierda ...... G...... T..A...... [585] C.costatus_oreistes_104408_Jacmel ...... G..T... ..T..A...... T...... T. [585] C.costatus_oreistes_192480_Puerto_Escondido ...... G..T... ..T..A...... T...... T. [585] C.sepsoides_102369_Sabana_de_La_Mar ....T...... C...... T...... [585] C.sepsoides_191850_Pestel ------[585] C.sepsoides_191913_Marche_Leon ------[585] C.sepsoides_192469_La_Zanga ------[585] C.sepsoides_193201_Tenares ------[585] C.sepsoides_266748_Jacmel ------[585] C.sepsoides_266749_Jacmel ------[585] C.stenurus_stenurus_103900_Rampe_des_Lions_Boios_Sec ------[585] C.stenurus_stenurus_103901_Rampe_des_Lions_Boios_Sec ------[585] C.stenurus_stenurus_103902_Rampe_des_Lions_Boios_Sec ------[585] C.stenurus_stenurus_267494_lle_a_Vache ------[585] C.haetiana_surdus_104418_Gros_Cheval ------[585] C.haetiana_surdus_104419_Gros_Cheval ------[585] C.haetiana_surdus_192486_Puerto_Escondido ------[585] C.haetiana_surdus_192497_Puerto_Escondido ------[585]

169

C.stenurus_rugosus_101247_Sabana_de_La_Mar ------[585] C.stenurus_rugosus_101248_Sabana_de_La_Mar ------[585] C.stenurus_rugosus_102371_El_Valle .T...... C..T. .G...... [585] C.stenurus_rugosus_103075_Las_Galeras .T...... C..T. .G...... [585] C.stenurus_rugosus_103083_Nagua .T...... C..T...... [585] C.stenurus_rugosus_103084_Nagua ------[585] C.stenurus_rugosus_193191_San_Francisco_de_Macoris ------[585] C.stenurus_rugosus_193192_San_Francisco_de_Macoris ------[585] C.stenurus_rugosus_193197_Tenares ------[585] C.stenurus_rugosus_193198_Tenares ------[585] C.stenurus_rugosus_193216_Tenares ------[585] C.stenurus_rugosus_193217_Tenares ------[585] C.warreni_172914_Hispaniola ...... C...... T... [585] C.warreni_194521_Puerto_Plata ...... C...... T... [585] C.stenurus_weinlandi_102712_Canoa ------[585] C.stenurus_weinlandi_103607_Soliette ------[585] C.stenurus_weinlandi_191591_Thomaseau ------[585] C.stenurus_weinlandi_191622_Thomaseau .T...... C..T. .G..T...... G. [585] C.stenurus_weinlandi_192405_Thomaseau .T...... C..T. .G..T...... G. [585] C.stenurus_weinlandi_266286_El_Azul .T...... C..T. .G..T...... G. [585] C.stenurus_weinlandi_266682_La_Zurza .T...... C..T. .G..T...... G. [585] C.stenurus_weinlandi_266684_La_Zurza ------[585] C.stenurus_weinlandi_266685_La_Zurza ------[585] D.bilobatus_194940_Costa_Rica ------[585]

170

D.nigropunctatus_191015_San_Luis_de_Potosi ...... C .....C..C...... [585] D.nigropunctatus_191319_Los_Calderos ....G...... C .....C..C...... [585] D.nigropunctatus_191320_Los_Calderos ....G...... C .....C..C...... [585] D.nigropunctatus_191542_San_Vincente ...... C .....C..C...... [585] D.garridoi_193507_El_Manguito ------[585] D.pleii_191574_Rio_Abajo ...... C ..T..C...... T....T ..... [585] C.barbouri_161120_Quick_Step .T...... G.C...... [585] C.barbouri_190678_Tejunde .T...... G.C...... [585] C.crusculus_101571_Ewarton .T...... G.C...... T... [585] C.crusculus_101572_Content .T...... G.C...... T... [585] C.crusculus_172442_Duncans ...... G.C...... T... [585] C.crusculus_101554_Town_Head ------[585] C.crusculus_103448_Knoxwood .T...... C...... A...... [585] C.crusculus_103449_Knowxwood ------[585] C.crusculus_103460_Jackson_Bay ...... C...... T... [585] C.crusculus_103586_Jackson_Bay ------[585] C.crusculus_161123_Duncans ------[585] C.crusculus_161124_Salt_Gut ...... G.C...... T... [585] C.crusculus_171439_Oracabessa ------[585] C.crusculus_171601_Port_Maria ------[585] C.crusculus_171603_Port_Maria ------[585] C.crusculus_171604_Port_Maria ------[585] C.crusculus_171605_Port_Maria ------[585] C.crusculus_171606_Oracabessa ------[585]

171

C.crusculus_171607_Braeton .T...... C...... G. [585] C.crusculus_172438_Oracabessa ------[585] C.crusculus_172441_Old_Hope ------[585] C.crusculus_172465_Section .T...... C...... T... [585] C.duquesneyi_267952_Hellshire_Hills .T...... G.....T...... C...... T. [585] C.hewardii_267097_Mandeville .T..G...... G.A...... [585] C.crusculus_101573_Hanover_Content ------[585] C.crusculus_101615_Oracabessa ------[585] C.crusculus_172439_Duncans ------[585] C.crusculus_172440_Old_Hope ------[585] C.crusculus_190695_Duncans ------[585] C.sepsoides_193116_Majagual ------[585] C.sepsoides_193117_Majagual ------[585] C.sepsoides_193118_Majagual ------[585] C.sepsoides_193202_Tenares ------[585] C.sepsoides_193203_Tenares ------[585] C.sepsoides_193204_Tenares ------[585] C.sepsoides_193040_Cotui ------[585] C.sepsoides_193041_Cotui ------[585] C.sepsoides_193042_Cotui ------[585] C.sepsoides_191851_Pestel ------[585] C.sepsoides_191914_Marche_Leon ------[585] C.sepsoides_191915_Marche_Leon ------[585] C.sepsoides_192313_Baraderes ------[585]

172

D.pleii_191575_Rio_Abajo ------[585] D.pleii_191576_Rio_Abajo ------[585] O.apodus_194938 ..T...... G.CA...... T..T.. C..G. [585]

C.agasepsoides_160191_Canoa CACTACTAAA CCT- [599] C.agasepsoides_194829_Bucan_Detwi ------[599] C.agasepsoides_194830_Bucan_Detwi ------[599] C.curtissi_aporus_102609_La_Descubierta ------[599] C.curtissi_aporus_102610_La_Descubierta .C...... - [599] C.curtissi_aporus_102611_La_Descubierta ------[599] C.curtissi_aporus_102706_Barahona ------[599] C.curtissi_aporus_102707_Juancho ------[599] C.curtissi_aporus_102709_Juancho ------[599] C.curtissi_aporus_102716_Barahona ------[599] C.curtissi_aporus_102915_Los_Arroyos ------[599] C.curtissi_aporus_102930_Barahona .C...... - [599] C.curtissi_aporus_194831_Bucan_Detwi .C...... - [599] C.curtissi_aporus_194832_Bucan_Detwi ------[599] C.curtissi_aporus_194833_Bucan_Detwi ------[599] C.curtissi_aporus_194838_Bucan_Detwi .C...... - [599] C.badius_194964_Navassa_Island ...... - [599] C.costatus_costatus_103085_Castillion ------[599] C.costatus_costatus_104113_Castillion .C..G...... - [599] C.costatus_costatus_192054_Abricots .C..G...... - [599] C.costatus_costatus_192260_Beaumont ------[599] C.cyanochloris_194930_Costa_Rica .T...... - [599] C.curtissi_curtissi_194503_La_Descubierta .C...... - [599] C.darlingtoni_161686_Cacique_Enriquillo .C...... - [599] C.darlingtoni_161687_Cacique_Enriquillo .C...... - [599] C.darlingtoni_161688_Cacique_Enriquillo .C...... - [599] C.curtissi_diastatus_194553_Mole_St._Nicolas .T...... - [599] C.curtissi_diastatus_194580_Mole_St._Nicolas ------[599] C.enneagrammus_191044_Mexico .C...T...... - [599] C.maculatus_266550_Cayman_Brac_West ------[599] C.maculatus_266551_Cayman_Brac_West ------[599] C.maculatus_266552_Cayman_Brac_West ------[599] C.maculatus_266553_Cayman_Brac_West ------[599] C.maculatus_266527_Cayman_Brac_West ...... - [599] C.marcanoi_193279_Valle_de_Bao .C.....T.. ...- [599] C.marcanoi_193280_Valle_de_Bao ------[599] C.marcanoi_193286_Valle_de_Bao .C.....T.. ...- [599] C.marcanoi_193309_Valle_de_Bao ------[599] C.marcanoi_193310_Valle_de_Bao ------[599] C.haetiana_mylicus_102546_Cabral ...... - [599] C.haetiana_mylicus_102547_Cabral ------[599]

173

C.haetiana_mylicus_102550_Cabral ------[599] C.haetiana_mylicus_160201_Tejunde ------[599] C.costatus_neiba_102922_La_Descubierta .C...... - [599] C.costatus_neiba_102923_Los_Pinos ------[599] C.costatus_neiba_102924_Los_Pinos ------[599] C.costatus_neiba_102925_Los_Pinos ------[599] C.costatus_neiba_161498_Cacique_Enriquillo .C...... - [599] C._costatus_neiba_194362_Apolinar_Pelodroma ------[599] C.costatus_neiba_194363_Apolinar_Pelodroma ------[599] C.costatus_neiba_266321_Rosa_de_La_Pierda .C...... - [599] C.costatus_neiba_266683_Rosa_de_La_Pierda .C...... - [599] C.costatus_oreistes_104408_Jacmel .C...... - [599] C.costatus_oreistes_192480_Puerto_Escondido .C...... - [599] C.sepsoides_102369_Sabana_de_La_Mar ...... T.. ...- [599] C.sepsoides_191850_Pestel ------[599] C.sepsoides_191913_Marche_Leon ------[599] C.sepsoides_192469_La_Zanga ------[599] C.sepsoides_193201_Tenares ------[599] C.sepsoides_266748_Jacmel ------[599] C.sepsoides_266749_Jacmel ------[599] C.stenurus_stenurus_103900_Rampe_des_Lions_Boios_Sec ------[599] C.stenurus_stenurus_103901_Rampe_des_Lions_Boios_Sec ------[599] C.stenurus_stenurus_103902_Rampe_des_Lions_Boios_Sec ------[599] C.stenurus_stenurus_267494_lle_a_Vache ------[599] C.haetiana_surdus_104418_Gros_Cheval ------[599] C.haetiana_surdus_104419_Gros_Cheval ------[599] C.haetiana_surdus_192486_Puerto_Escondido ------[599] C.haetiana_surdus_192497_Puerto_Escondido ------[599] C.stenurus_rugosus_101247_Sabana_de_La_Mar ------[599] C.stenurus_rugosus_101248_Sabana_de_La_Mar ------[599] C.stenurus_rugosus_102371_El_Valle ...... - [599] C.stenurus_rugosus_103075_Las_Galeras ...... - [599] C.stenurus_rugosus_103083_Nagua ...... - [599] C.stenurus_rugosus_103084_Nagua ------[599] C.stenurus_rugosus_193191_San_Francisco_de_Macoris ------[599] C.stenurus_rugosus_193192_San_Francisco_de_Macoris ------[599] C.stenurus_rugosus_193197_Tenares ------[599] C.stenurus_rugosus_193198_Tenares ------[599] C.stenurus_rugosus_193216_Tenares ------[599] C.stenurus_rugosus_193217_Tenares ------[599] C.warreni_172914_Hispaniola .C...... - [599] C.warreni_194521_Puerto_Plata .C...... - [599] C.stenurus_weinlandi_102712_Canoa ------[599] C.stenurus_weinlandi_103607_Soliette ------[599] C.stenurus_weinlandi_191591_Thomaseau ------[599] C.stenurus_weinlandi_191622_Thomaseau ...... - [599] C.stenurus_weinlandi_192405_Thomaseau ...... - [599] C.stenurus_weinlandi_266286_El_Azul ...... - [599]

174

C.stenurus_weinlandi_266682_La_Zurza ...... - [599] C.stenurus_weinlandi_266684_La_Zurza ------[599] C.stenurus_weinlandi_266685_La_Zurza ------[599] D.bilobatus_194940_Costa_Rica ------[599] D.nigropunctatus_191015_San_Luis_de_Potosi .C..G..C.. .G.- [599] D.nigropunctatus_191319_Los_Calderos .C..G..C.. .A.- [599] D.nigropunctatus_191320_Los_Calderos .C..G..C.. .A.- [599] D.nigropunctatus_191542_San_Vincente .C..G..C.. .A.- [599] D.garridoi_193507_El_Manguito ------[599] D.pleii_191574_Rio_Abajo .T.....C.. .G.- [599] C.barbouri_161120_Quick_Step .G...... - [599] C.barbouri_190678_Tejunde .G...... - [599] C.crusculus_101571_Ewarton ...... - [599] C.crusculus_101572_Content ...... - [599] C.crusculus_172442_Duncans ...... - [599] C.crusculus_101554_Town_Head ------[599] C.crusculus_103448_Knoxwood .G...... - [599] C.crusculus_103449_Knowxwood ------[599] C.crusculus_103460_Jackson_Bay ...... - [599] C.crusculus_103586_Jackson_Bay ------[599] C.crusculus_161123_Duncans ------[599] C.crusculus_161124_Salt_Gut ...... - [599] C.crusculus_171439_Oracabessa ------[599] C.crusculus_171601_Port_Maria ------[599] C.crusculus_171603_Port_Maria ------[599] C.crusculus_171604_Port_Maria ------[599] C.crusculus_171605_Port_Maria ------[599] C.crusculus_171606_Oracabessa ------[599] C.crusculus_171607_Braeton ...... - [599] C.crusculus_172438_Oracabessa ------[599] C.crusculus_172441_Old_Hope ------[599] C.crusculus_172465_Section .T...... - [599] C.duquesneyi_267952_Hellshire_Hills .G.....G.. ...- [599] C.hewardii_267097_Mandeville ...... - [599] C.crusculus_101573_Hanover_Content ------[599] C.crusculus_101615_Oracabessa ------[599] C.crusculus_172439_Duncans ------[599] C.crusculus_172440_Old_Hope ------[599] C.crusculus_190695_Duncans ------[599] C.sepsoides_193116_Majagual ------[599] C.sepsoides_193117_Majagual ------[599] C.sepsoides_193118_Majagual ------[599] C.sepsoides_193202_Tenares ------[599] C.sepsoides_193203_Tenares ------[599] C.sepsoides_193204_Tenares ------[599] C.sepsoides_193040_Cotui ------[599] C.sepsoides_193041_Cotui ------[599] C.sepsoides_193042_Cotui ------[599]

175

C.sepsoides_191851_Pestel ------[599] C.sepsoides_191914_Marche_Leon ------[599] C.sepsoides_191915_Marche_Leon ------[599] C.sepsoides_192313_Baraderes ------[599] D.pleii_191575_Rio_Abajo ------[599] D.pleii_191576_Rio_Abajo ------[599] O.apodus_194938 .C..T..C.. ...- [599]

176

APPENDIX E. RAG1 nucDNA SEQUENCE DATA.

C.agasepsoides_160191_Canoa GTTGGAGATT GGTGAGGTGT ACAAAAATCC TAACGCTACG AAAGA [ 45] C.curtissi_aporus_102610_La_Descubierta .A...... A.G...... [ 45] C.curtissi_aporus_102930_Barahona .A...... A.G...... [ 45] C.curtissi_aporus_194831_Bucan_Detwi .A...... A.G...... [ 45] C.badius_194964_Navassa_Island .A...... A.G...... [ 45] C.barbouri_161120_Quick_Step .A...... A.G...... [ 45] C.costatus_costatus_104113_Castillion .A...... A.G...... [ 45] C.costatus_costatus_192054_Abricots .A...... A.G...... [ 45] C.crusculus_101572_Content .A...... A.G...... [ 45] C.crusculus_103448_Knoxwood .A...... A.G...... [ 45] C.crusculus_103460_Jackson_Bay .A...... A.G...... [ 45] C.crusculus_161124_Salt_Gut .A...... A.G...... [ 45] C.crusculus_171607_Braeton .A...... A.G...... [ 45] C.crusculus_172442_Duncans .A...... C...A.G...... [ 45] C.crusculus_172465_Section .A...... A.G...... [ 45] C.curtissi_curtissi_194503_La_Descubierta .A...... A.G...... [ 45] C.cyanochloris_194930_Costa_Rica ...T...... A...... C.. A.....GT...... [ 45] C.darlingtoni_161687_Cacique_Enriguillo ...... A.G...... [ 45] C.curtissi_diastatus_194553_Mole_St._Nicolas .A...... A.G...... [ 45] C.duquesneyi_267952_Hellshire_Hills .A...... A.G...... [ 45] C.enneagrammus_191044_Mexico ...T...... C..A...... C.. G.....GT...... [ 45] C.hewardi_267097_Mandeville .A...... A.G...... [ 45]

177

C.maculatus_266527_Cayman_Brac .A...... A.G...... [ 45] C.maculatus_266550_Cayman_Brac .A...... A.G...... [ 45] C.marcanoi_193286_Valle_de_Baco .A...... A...... A.G...... [ 45] C.marcanoi_193309_La_Lagunas .A...... A.G...... [ 45] C.haetianus_mylicus_102465_Cabral ...... A.G...... [ 45] C.costatus_neiba_102922_La_Descubierta .A...... A.G...... [ 45] C.costatus_neiba_192428_Ca_Soleil .A...... A.G...... [ 45] C.costatus_neiba_266321_Rosa_de_La_Pierda .A...... A.G...... [ 45] C.costatus_neiba_266683_La_Zurza .A...... C...A.G...... [ 45] C.costatus_oreistes_192480_Puerto_Escondido .A...... A.G...... [ 45] C.costatus_oreistes_104408_Jacmel .A...... A.G...... [ 45] C.stenurus_rugosus_102371_El_Valle .A...... C...A.G...... [ 45] C.stenurus_rugosus_103083_Nagua .A...... C...A.G...... [ 45] C.sepsoides_102369_Sabana_de_La_Mar ...... A.G...... [ 45] C.warreni_172914_Hispaniola .A...... A.G...... [ 45] C.warreni_194521_Puerto_Plata ...... A.G...... [ 45] C.stenurus_weinlandi_192405_Thomaseau .A...... C...A.G...... [ 45] C.stenurus_weinlandi_266286_El_Azul .A...... A.G...... [ 45] C.stenurus_weinlandi_266682_La_Zurza .A...... C...A.G...... [ 45] D.bilobatus_194940_Costa_Rica ...T...... C..A...... C.G..C.. C.....GT...... [ 45] D.garridoi_193507_Cuba ...T...... A...... G..C.. C.....AT.. .C... [ 45] D.nigropunctatus_191320_Cuba ...T...... A...... G..C.. C.....AT.. .C... [ 45] D.nigropunctatus_191542_Cuba ...T...... A...... G..C.. C.....AT.. .C... [ 45] D.nigropuntatus_191015_Cuba ...T...... A...... G..C.. C.....AT.. .C... [ 45]

178

D.pleii_191574_Puerto_Rico ...T...... A...... G..C.. C.....AT.. .C... [ 45] O.apodus_194938 ...T...... C..A...... C.. .G.T..G...... [ 45] C.agasepsoides_160191_Canoa AGAGAGAAAG AGGTGGCAAT CGACTCTTGA CAAGCACCTC AGAAA [ 90] C.curtissi_aporus_102610_La_Descubierta ...... A...... A...... [ 90] C.curtissi_aporus_102930_Barahona ...... A...... A...... [ 90] C.curtissi_aporus_194831_Bucan_Detwi ...... A...... A...... [ 90] C.badius_194964_Navassa_Island ...... A...... A...... A... [ 90] C.barbouri_161120_Quick_Step ...... A...... [ 90] C.costatus_costatus_104113_Castillion ...... A...... [ 90] C.costatus_costatus_192054_Abricots ...... A...... A...... [ 90] C.crusculus_101572_Content ...... A...... [ 90] C.crusculus_103448_Knoxwood ...... A...... A...... [ 90] C.crusculus_103460_Jackson_Bay ...... A...... A...... [ 90] C.crusculus_161124_Salt_Gut ...... A...... [ 90] C.crusculus_171607_Braeton ...... A...... [ 90] C.crusculus_172442_Duncans ...... A...... A...... [ 90] C.crusculus_172465_Section ...... A...... A...... [ 90] C.curtissi_curtissi_194503_La_Descubierta ...... A...... A...... [ 90] C.cyanochloris_194930_Costa_Rica ...... G. .A...... A...... [ 90] C.darlingtoni_161687_Cacique_Enriguillo ...... A...... A...... [ 90] C.curtissi_diastatus_194553_Mole_St._Nicolas ...... A...... A...... [ 90] C.duquesneyi_267952_Hellshire_Hills .....A.... .AAA...... A...... A...... [ 90] C.enneagrammus_191044_Mexico ...... G. .A...... A...... [ 90] C.hewardi_267097_Mandeville ...... A...... A...... [ 90]

179

C.maculatus_266527_Cayman_Brac ...... A...... A...... [ 90] C.maculatus_266550_Cayman_Brac ...... A...... A...... [ 90] C.marcanoi_193286_Valle_de_Baco ...... A...... A...... [ 90] C.marcanoi_193309_La_Lagunas ...... A...... A...... [ 90] C.haetianus_mylicus_102465_Cabral ...... A...... [ 90] C.costatus_neiba_102922_La_Descubierta ...... A...... A...... [ 90] C.costatus_neiba_192428_Ca_Soleil ...... A...... A...... [ 90] C.costatus_neiba_266321_Rosa_de_La_Pierda ...... A...... A...... [ 90] C.costatus_neiba_266683_La_Zurza ...... A...... A...... [ 90] C.costatus_oreistes_192480_Puerto_Escondido ...... A...... A...... [ 90] C.costatus_oreistes_104408_Jacmel ...... A...... A...... [ 90] C.stenurus_rugosus_102371_El_Valle ...... A...... A...... [ 90] C.stenurus_rugosus_103083_Nagua ...... A...... A...... [ 90] C.sepsoides_102369_Sabana_de_La_Mar ...... A...... [ 90] C.warreni_172914_Hispaniola ...... A...... A...... [ 90] C.warreni_194521_Puerto_Plata ...... A...... A...... [ 90] C.stenurus_weinlandi_192405_Thomaseau ...... A...... A...... [ 90] C.stenurus_weinlandi_266286_El_Azul ...... A...... A...... [ 90] C.stenurus_weinlandi_266682_La_Zurza ...... A...... A...... [ 90] D.bilobatus_194940_Costa_Rica ...... G. .A.....G.. ...A..T... ..G.. [ 90] D.garridoi_193507_Cuba ...... G. .AGTG...... A...... G.. [ 90] D.nigropunctatus_191320_Cuba ...... G. .AGTG...... A...... G.. [ 90] D.nigropunctatus_191542_Cuba ...... G. .AGTG...... A...... G.. [ 90] D.nigropuntatus_191015_Cuba ...... G. .AGTG...... A...... G.. [ 90]

180

D.pleii_191574_Puerto_Rico ...... G. .A.TG...... A...... G.. [ 90] O.apodus_194938 ...... G. .A...... A...... [ 90] C.agasepsoides_160191_Canoa AAAGATGAAT CTCAAGCCTG TAACAAGGAT GAATGGGAAT TTTGC [135] C.curtissi_aporus_102610_La_Descubierta ...... A...... A...... [135] C.curtissi_aporus_102930_Barahona ...... A...... A...... [135] C.curtissi_aporus_194831_Bucan_Detwi ...... A...... A...... [135] C.badius_194964_Navassa_Island ...... GA ..A...... A...... [135] C.barbouri_161120_Quick_Step ...... A...... G...... A...... [135] C.costatus_costatus_104113_Castillion ...... A...... G...... A...... [135] C.costatus_costatus_192054_Abricots ...... A...... A...... [135] C.crusculus_101572_Content ...... A...... G...... A...... [135] C.crusculus_103448_Knoxwood ...... A...... A...... [135] C.crusculus_103460_Jackson_Bay ...... A...... A...... [135] C.crusculus_161124_Salt_Gut ...... A...... G...... A...... [135] C.crusculus_171607_Braeton ...... A...... A...... [135] C.crusculus_172442_Duncans ...... A...... A...... [135] C.crusculus_172465_Section ...... A...... G...... A...... [135] C.curtissi_curtissi_194503_La_Descubierta ...... A...... A...... [135] C.cyanochloris_194930_Costa_Rica G...... C ..A..A...A ...... A...... [135] C.darlingtoni_161687_Cacique_Enriguillo ...... A...... A...... [135] C.curtissi_diastatus_194553_Mole_St._Nicolas ...... A...... A...... [135] C.duquesneyi_267952_Hellshire_Hills ...... A...... A...... [135] C.enneagrammus_191044_Mexico G...G....C ..A..A...... G...... [135] C.hewardi_267097_Mandeville ...... A...... A...... [135]

181

C.maculatus_266527_Cayman_Brac ...... A...... G...... A...... [135] C.maculatus_266550_Cayman_Brac ...... A...... G...... A...... [135] C.marcanoi_193286_Valle_de_Baco ...... A...... A...... [135] C.marcanoi_193309_La_Lagunas ...... A...... A...... [135] C.haetianus_mylicus_102465_Cabral ...... [135] C.costatus_neiba_102922_La_Descubierta ...... A...... A...... [135] C.costatus_neiba_192428_Ca_Soleil ...... A...... A...... [135] C.costatus_neiba_266321_Rosa_de_La_Pierda ...... A...... A...... [135] C.costatus_neiba_266683_La_Zurza ...... A...... G...... A...... [135] C.costatus_oreistes_192480_Puerto_Escondido ...... A...... A...... [135] C.costatus_oreistes_104408_Jacmel ...... A...... A...... [135] C.stenurus_rugosus_102371_El_Valle ...... A...... G...... A...... [135] C.stenurus_rugosus_103083_Nagua ...... A...... A...... [135] C.sepsoides_102369_Sabana_de_La_Mar ...... A...... [135] C.warreni_172914_Hispaniola ...... A...... A...... [135] C.warreni_194521_Puerto_Plata ...... A...... A...... [135] C.stenurus_weinlandi_192405_Thomaseau ...... A...... G...... A...... [135] C.stenurus_weinlandi_266286_El_Azul ...... A...... A...... [135] C.stenurus_weinlandi_266682_La_Zurza ...... A...... G...... A...... [135] D.bilobatus_194940_Costa_Rica G...G....C ...... A ...... [135] D.garridoi_193507_Cuba G...G....C ..A...... A...... [135] D.nigropunctatus_191320_Cuba G...G....C ..A...... A...... [135] D.nigropunctatus_191542_Cuba G...G....C ..A...... A...... [135] D.nigropuntatus_191015_Cuba G...G....C ..A...... A...... [135]

182

D.pleii_191574_Puerto_Rico G...G....C ..A...... A...... [135] O.apodus_194938 G...... C ..G...... A...... [135] C.agasepsoides_160191_Canoa TAGGAAGCTT ATGACCAAGG AGACCGTGGA AGCAGTTTGT GAACT [180] C.curtissi_aporus_102610_La_Descubierta ...A...... [180] C.curtissi_aporus_102930_Barahona ...A...... [180] C.curtissi_aporus_194831_Bucan_Detwi ...A...... [180] C.badius_194964_Navassa_Island ...A...... [180] C.barbouri_161120_Quick_Step ...A...... [180] C.costatus_costatus_104113_Castillion ...A...... [180] C.costatus_costatus_192054_Abricots ...A...... [180] C.crusculus_101572_Content ...A...... [180] C.crusculus_103448_Knoxwood ...A...... [180] C.crusculus_103460_Jackson_Bay ...A...... T...... [180] C.crusculus_161124_Salt_Gut ...A...... [180] C.crusculus_171607_Braeton ...A...... [180] C.crusculus_172442_Duncans ...A...... [180] C.crusculus_172465_Section ...A...... [180] C.curtissi_curtissi_194503_La_Descubierta ...A...... [180] C.cyanochloris_194930_Costa_Rica ...A...... A...... [180] C.darlingtoni_161687_Cacique_Enriguillo ...A...... [180] C.curtissi_diastatus_194553_Mole_St._Nicolas ...A...... [180] C.duquesneyi_267952_Hellshire_Hills ...A...... [180] C.enneagrammus_191044_Mexico ...A...... T...... [180] C.hewardi_267097_Mandeville ...A...... [180]

183

C.maculatus_266527_Cayman_Brac ...A...... [180] C.maculatus_266550_Cayman_Brac ...A...... [180] C.marcanoi_193286_Valle_de_Baco ...A...... [180] C.marcanoi_193309_La_Lagunas ...A...... [180] C.haetianus_mylicus_102465_Cabral ...A...... [180] C.costatus_neiba_102922_La_Descubierta ...A...... [180] C.costatus_neiba_192428_Ca_Soleil ...A...... [180] C.costatus_neiba_266321_Rosa_de_La_Pierda ...A...... [180] C.costatus_neiba_266683_La_Zurza ...A...... [180] C.costatus_oreistes_192480_Puerto_Escondido ...A...... [180] C.costatus_oreistes_104408_Jacmel ...A...... [180] C.stenurus_rugosus_102371_El_Valle ...A...... [180] C.stenurus_rugosus_103083_Nagua ...A...... [180] C.sepsoides_102369_Sabana_de_La_Mar ...A...... [180] C.warreni_172914_Hispaniola ...A...... [180] C.warreni_194521_Puerto_Plata ...A...... [180] C.stenurus_weinlandi_192405_Thomaseau ...A...... [180] C.stenurus_weinlandi_266286_El_Azul ...A...... [180] C.stenurus_weinlandi_266682_La_Zurza ...A...... [180] D.bilobatus_194940_Costa_Rica ...A.....G ...... T...... [180] D.garridoi_193507_Cuba ...A...... G...... T...... [180] D.nigropunctatus_191320_Cuba ...A...... G...... T...... [180] D.nigropunctatus_191542_Cuba ...A...... G...... T...... [180] D.nigropuntatus_191015_Cuba ...A...... G...... T...... [180]

184

D.pleii_191574_Puerto_Rico ...... G...... T...... [180] O.apodus_194938 ...A.....C ...T...... T...... [180] C.agasepsoides_160191_Canoa GGTCATGTGC GAGGAGAGGC ATGAAGTCCT CAGAGAACTC ATGGA [225] C.curtissi_aporus_102610_La_Descubierta ...... [225] C.curtissi_aporus_102930_Barahona ...... [225] C.curtissi_aporus_194831_Bucan_Detwi ...... [225] C.badius_194964_Navassa_Island ...... C...... AC [225] C.barbouri_161120_Quick_Step ...... [225] C.costatus_costatus_104113_Castillion ...... [225] C.costatus_costatus_192054_Abricots ...... [225] C.crusculus_101572_Content ...... [225] C.crusculus_103448_Knoxwood ...... T ...... [225] C.crusculus_103460_Jackson_Bay ...... [225] C.crusculus_161124_Salt_Gut ...... [225] C.crusculus_171607_Braeton ...... [225] C.crusculus_172442_Duncans ...... [225] C.crusculus_172465_Section ...... T ...... [225] C.curtissi_curtissi_194503_La_Descubierta ...... [225] C.cyanochloris_194930_Costa_Rica .....A...T ...... C...... [225] C.darlingtoni_161687_Cacique_Enriguillo ...... [225] C.curtissi_diastatus_194553_Mole_St._Nicolas ...... [225] C.duquesneyi_267952_Hellshire_Hills ...... [225] C.enneagrammus_191044_Mexico ...T.....T ...... C....C...... [225] C.hewardi_267097_Mandeville ...... [225]

185

C.maculatus_266527_Cayman_Brac ...... [225] C.maculatus_266550_Cayman_Brac ...... [225] C.marcanoi_193286_Valle_de_Baco ...... [225] C.marcanoi_193309_La_Lagunas ...... [225] C.haetianus_mylicus_102465_Cabral ...... [225] C.costatus_neiba_102922_La_Descubierta ...... [225] C.costatus_neiba_192428_Ca_Soleil ...... [225] C.costatus_neiba_266321_Rosa_de_La_Pierda ...... [225] C.costatus_neiba_266683_La_Zurza ...... [225] C.costatus_oreistes_192480_Puerto_Escondido ...... [225] C.costatus_oreistes_104408_Jacmel ...... [225] C.stenurus_rugosus_102371_El_Valle ...... A...... [225] C.stenurus_rugosus_103083_Nagua ...... [225] C.sepsoides_102369_Sabana_de_La_Mar ...... [225] C.warreni_172914_Hispaniola ...... [225] C.warreni_194521_Puerto_Plata ...... [225] C.stenurus_weinlandi_192405_Thomaseau ...... [225] C.stenurus_weinlandi_266286_El_Azul ...... [225] C.stenurus_weinlandi_266682_La_Zurza ...... [225] D.bilobatus_194940_Costa_Rica .....A...T ...... T.C...... A. [225] D.garridoi_193507_Cuba .A...A...T ...... C.G...... [225] D.nigropunctatus_191320_Cuba .A...A...T ...... C.G...... [225] D.nigropunctatus_191542_Cuba .A...A...T ...... C.G...... [225] D.nigropuntatus_191015_Cuba .A...A...T ...... C.G...... [225]

186

D.pleii_191574_Puerto_Rico .A...A...T ...... C...... G...... [225] O.apodus_194938 .....A...T ...... C...... G...... [225] C.agasepsoides_160191_Canoa CCTTTATCTT AAGATGAAAC CAGTCTGGCG GTCTTCATGT CCCAC [270] C.curtissi_aporus_102610_La_Descubierta ...... G...... [270] C.curtissi_aporus_102930_Barahona ...... G...... [270] C.curtissi_aporus_194831_Bucan_Detwi ...... G...... [270] C.badius_194964_Navassa_Island ...... G...... [270] C.barbouri_161120_Quick_Step ...... G...... [270] C.costatus_costatus_104113_Castillion ...... G...... [270] C.costatus_costatus_192054_Abricots ...... G...... [270] C.crusculus_101572_Content ...... G...... [270] C.crusculus_103448_Knoxwood ...... G...... [270] C.crusculus_103460_Jackson_Bay ...... G...... [270] C.crusculus_161124_Salt_Gut ...... G...... [270] C.crusculus_171607_Braeton ...... G...... [270] C.crusculus_172442_Duncans ...... G...... [270] C.crusculus_172465_Section ...... A...... G...... [270] C.curtissi_curtissi_194503_La_Descubierta ...... G...... [270] C.cyanochloris_194930_Costa_Rica ...... G ...... A...... [270] C.darlingtoni_161687_Cacique_Enriguillo ...... [270] C.curtissi_diastatus_194553_Mole_St._Nicolas ...... G...... [270] C.duquesneyi_267952_Hellshire_Hills ...... G...... [270] C.enneagrammus_191044_Mexico ...... C..G ...... [270] C.hewardi_267097_Mandeville ...... G...... [270]

187

C.maculatus_266527_Cayman_Brac ...... G...... [270] C.maculatus_266550_Cayman_Brac ...... G...... [270] C.marcanoi_193286_Valle_de_Baco ...... G...... [270] C.marcanoi_193309_La_Lagunas ...... G...... [270] C.haetianus_mylicus_102465_Cabral ...... [270] C.costatus_neiba_102922_La_Descubierta ...... [270] C.costatus_neiba_192428_Ca_Soleil ...... G...... [270] C.costatus_neiba_266321_Rosa_de_La_Pierda ...... G...... [270] C.costatus_neiba_266683_La_Zurza ...... G...... [270] C.costatus_oreistes_192480_Puerto_Escondido ...... G...... [270] C.costatus_oreistes_104408_Jacmel ...... G...... [270] C.stenurus_rugosus_102371_El_Valle ...... G...... [270] C.stenurus_rugosus_103083_Nagua ...... G...... [270] C.sepsoides_102369_Sabana_de_La_Mar ...... G...... [270] C.warreni_172914_Hispaniola ...... G...... [270] C.warreni_194521_Puerto_Plata ...... [270] C.stenurus_weinlandi_192405_Thomaseau ...... G...... [270] C.stenurus_weinlandi_266286_El_Azul ...... G...... [270] C.stenurus_weinlandi_266682_La_Zurza ...... G...... [270] D.bilobatus_194940_Costa_Rica ...... C..G...... [270] D.garridoi_193507_Cuba ...... A...... T [270] D.nigropunctatus_191320_Cuba ...... A...... T [270] D.nigropunctatus_191542_Cuba ...... A...... T [270] D.nigropuntatus_191015_Cuba ...... A...... T [270]

188

D.pleii_191574_Puerto_Rico ...... A...... [270] O.apodus_194938 ...... A...... [270] C.agasepsoides_160191_Canoa TAAGGAATGC CCAGAACTGG TATGCCAGTA CAGTTTCAAC TCTCA [315] C.curtissi_aporus_102610_La_Descubierta ...... [315] C.curtissi_aporus_102930_Barahona ...... [315] C.curtissi_aporus_194831_Bucan_Detwi ...... [315] C.badius_194964_Navassa_Island ...... G...... [315] C.barbouri_161120_Quick_Step ...... [315] C.costatus_costatus_104113_Castillion ...... [315] C.costatus_costatus_192054_Abricots ...... [315] C.crusculus_101572_Content ...... [315] C.crusculus_103448_Knoxwood ...... [315] C.crusculus_103460_Jackson_Bay ...... [315] C.crusculus_161124_Salt_Gut ...... [315] C.crusculus_171607_Braeton ...... [315] C.crusculus_172442_Duncans ...... [315] C.crusculus_172465_Section ...... [315] C.curtissi_curtissi_194503_La_Descubierta ...... [315] C.cyanochloris_194930_Costa_Rica ...... [315] C.darlingtoni_161687_Cacique_Enriguillo ...... [315] C.curtissi_diastatus_194553_Mole_St._Nicolas ...... [315] C.duquesneyi_267952_Hellshire_Hills ...... T...... [315] C.enneagrammus_191044_Mexico ...... [315] C.hewardi_267097_Mandeville ...... [315]

189

C.maculatus_266527_Cayman_Brac ...... [315] C.maculatus_266550_Cayman_Brac ...... [315] C.marcanoi_193286_Valle_de_Baco ...... [315] C.marcanoi_193309_La_Lagunas ...... [315] C.haetianus_mylicus_102465_Cabral ...... [315] C.costatus_neiba_102922_La_Descubierta ...... [315] C.costatus_neiba_192428_Ca_Soleil ...... [315] C.costatus_neiba_266321_Rosa_de_La_Pierda ...... [315] C.costatus_neiba_266683_La_Zurza ...... C.. [315] C.costatus_oreistes_192480_Puerto_Escondido ...... [315] C.costatus_oreistes_104408_Jacmel ...... [315] C.stenurus_rugosus_102371_El_Valle ...... C.. [315] C.stenurus_rugosus_103083_Nagua ...... C.. [315] C.sepsoides_102369_Sabana_de_La_Mar ...... [315] C.warreni_172914_Hispaniola ...... [315] C.warreni_194521_Puerto_Plata ...... T...... [315] C.stenurus_weinlandi_192405_Thomaseau ...... C.. [315] C.stenurus_weinlandi_266286_El_Azul ...... [315] C.stenurus_weinlandi_266682_La_Zurza ...... C.. [315] D.bilobatus_194940_Costa_Rica G...... G...... [315] D.garridoi_193507_Cuba G...... G.. [315] D.nigropunctatus_191320_Cuba G...... G.. [315] D.nigropunctatus_191542_Cuba G...... G.. [315] D.nigropuntatus_191015_Cuba G...... G.. [315]

190

D.pleii_191574_Puerto_Rico G...... G.. [315] O.apodus_194938 C...... T...... [315] C.agasepsoides_160191_Canoa ACGTTTCGCA GAGTTGCTGT CCACCAAGCT CAGGTACAGA TATGA [360] C.curtissi_aporus_102610_La_Descubierta ...... A...... [360] C.curtissi_aporus_102930_Barahona ...... A...... [360] C.curtissi_aporus_194831_Bucan_Detwi ...... A...... [360] C.badius_194964_Navassa_Island ...... A...... [360] C.barbouri_161120_Quick_Step ...... A...... [360] C.costatus_costatus_104113_Castillion ...... [360] C.costatus_costatus_192054_Abricots ...... A...... [360] C.crusculus_101572_Content ...... A...... [360] C.crusculus_103448_Knoxwood ...... A...... [360] C.crusculus_103460_Jackson_Bay ...... A...... C...... [360] C.crusculus_161124_Salt_Gut ...... [360] C.crusculus_171607_Braeton ...... A...... [360] C.crusculus_172442_Duncans ...... A...... [360] C.crusculus_172465_Section ...... A...... [360] C.curtissi_curtissi_194503_La_Descubierta ...... A...... [360] C.cyanochloris_194930_Costa_Rica ...... T...... T...T...... T...... [360] C.darlingtoni_161687_Cacique_Enriguillo ...... [360] C.curtissi_diastatus_194553_Mole_St._Nicolas ...... A...... [360] C.duquesneyi_267952_Hellshire_Hills ...... A...... [360] C.enneagrammus_191044_Mexico ...... T...... T...... T...... [360] C.hewardi_267097_Mandeville ...... A...... [360]

191

C.maculatus_266527_Cayman_Brac ...... [360] C.maculatus_266550_Cayman_Brac ...... [360] C.marcanoi_193286_Valle_de_Baco ...... A...... [360] C.marcanoi_193309_La_Lagunas ...... A...... [360] C.haetianus_mylicus_102465_Cabral ...... [360] C.costatus_neiba_102922_La_Descubierta ...... A...... [360] C.costatus_neiba_192428_Ca_Soleil ...... A...... A...... [360] C.costatus_neiba_266321_Rosa_de_La_Pierda ...... T...... A...... A...... [360] C.costatus_neiba_266683_La_Zurza ...... [360] C.costatus_oreistes_192480_Puerto_Escondido ...... A...... T... [360] C.costatus_oreistes_104408_Jacmel ...... A...... [360] C.stenurus_rugosus_102371_El_Valle ...... [360] C.stenurus_rugosus_103083_Nagua ...... [360] C.sepsoides_102369_Sabana_de_La_Mar ...... [360] C.warreni_172914_Hispaniola ...... A...... A...... [360] C.warreni_194521_Puerto_Plata ...... T...... [360] C.stenurus_weinlandi_192405_Thomaseau ...... [360] C.stenurus_weinlandi_266286_El_Azul ...... A...... A...... [360] C.stenurus_weinlandi_266682_La_Zurza ...... [360] D.bilobatus_194940_Costa_Rica ...... T...... T...... G ..... [360] D.garridoi_193507_Cuba G.....T...... T...... [360] D.nigropunctatus_191320_Cuba ...... T...... T...... [360] D.nigropunctatus_191542_Cuba ...... T...... T...... [360] D.nigropuntatus_191015_Cuba ...... T...... T...... [360]

192

D.pleii_191574_Puerto_Rico ...... T...... T...... [360] O.apodus_194938 ...... T...... A...T...... [360] C.agasepsoides_160191_Canoa TGGCAAGATT ACCAATTACT TTCACAAAAC GCTTGCCCAC GTTCC [405] C.curtissi_aporus_102610_La_Descubierta ...... [405] C.curtissi_aporus_102930_Barahona ...... [405] C.curtissi_aporus_194831_Bucan_Detwi ...... [405] C.badius_194964_Navassa_Island ...... [405] C.barbouri_161120_Quick_Step ...... [405] C.costatus_costatus_104113_Castillion ...... [405] C.costatus_costatus_192054_Abricots ...... A [405] C.crusculus_101572_Content ...... [405] C.crusculus_103448_Knoxwood ...... [405] C.crusculus_103460_Jackson_Bay ...... [405] C.crusculus_161124_Salt_Gut ...... [405] C.crusculus_171607_Braeton ...... [405] C.crusculus_172442_Duncans ...... [405] C.crusculus_172465_Section ...... [405] C.curtissi_curtissi_194503_La_Descubierta ...... [405] C.cyanochloris_194930_Costa_Rica ...... A...... T ..... [405] C.darlingtoni_161687_Cacique_Enriguillo ...... [405] C.curtissi_diastatus_194553_Mole_St._Nicolas ...... [405] C.duquesneyi_267952_Hellshire_Hills ...... T ..... [405] C.enneagrammus_191044_Mexico ...... G.....T ..... [405] C.hewardi_267097_Mandeville ...... [405]

193

C.maculatus_266527_Cayman_Brac ...... [405] C.maculatus_266550_Cayman_Brac ...... [405] C.marcanoi_193286_Valle_de_Baco ...... [405] C.marcanoi_193309_La_Lagunas ...... [405] C.haetianus_mylicus_102465_Cabral ...... [405] C.costatus_neiba_102922_La_Descubierta ...... [405] C.costatus_neiba_192428_Ca_Soleil ...... [405] C.costatus_neiba_266321_Rosa_de_La_Pierda ...... [405] C.costatus_neiba_266683_La_Zurza ...... G...... [405] C.costatus_oreistes_192480_Puerto_Escondido ...... [405] C.costatus_oreistes_104408_Jacmel ...... [405] C.stenurus_rugosus_102371_El_Valle ...... G...... [405] C.stenurus_rugosus_103083_Nagua ...... G...... [405] C.sepsoides_102369_Sabana_de_La_Mar A...... G...... [405] C.warreni_172914_Hispaniola ...... [405] C.warreni_194521_Puerto_Plata ...... [405] C.stenurus_weinlandi_192405_Thomaseau ...... G...... [405] C.stenurus_weinlandi_266286_El_Azul ...... [405] C.stenurus_weinlandi_266682_La_Zurza ...... G...... [405] D.bilobatus_194940_Costa_Rica ...... C...... A...... T ..... [405] D.garridoi_193507_Cuba ...... C...... A...... [405] D.nigropunctatus_191320_Cuba ...... A...... [405] D.nigropunctatus_191542_Cuba ...... C...... A...... [405] D.nigropuntatus_191015_Cuba A...... C...... A....G...... [405]

194

D.pleii_191574_Puerto_Rico ...... C...... A...... T ..... [405] O.apodus_194938 ...... T...... [405] C.agasepsoides_160191_Canoa AGAAATTATA GAAAGAGACG GCTCCATTGG TGCTTGGGCA AGTGA [450] C.curtissi_aporus_102610_La_Descubierta ...... [450] C.curtissi_aporus_102930_Barahona ...... [450] C.curtissi_aporus_194831_Bucan_Detwi ...... [450] C.badius_194964_Navassa_Island ...... [450] C.barbouri_161120_Quick_Step ...... [450] C.costatus_costatus_104113_Castillion ...... [450] C.costatus_costatus_192054_Abricots C...... [450] C.crusculus_101572_Content ...... T...... [450] C.crusculus_103448_Knoxwood ...... [450] C.crusculus_103460_Jackson_Bay ...... A..A .....T...... [450] C.crusculus_161124_Salt_Gut ...... [450] C.crusculus_171607_Braeton ...... [450] C.crusculus_172442_Duncans ...... [450] C.crusculus_172465_Section ...... [450] C.curtissi_curtissi_194503_La_Descubierta ...... [450] C.cyanochloris_194930_Costa_Rica ...... T...... [450] C.darlingtoni_161687_Cacique_Enriguillo ...... [450] C.curtissi_diastatus_194553_Mole_St._Nicolas ...... [450] C.duquesneyi_267952_Hellshire_Hills ...... [450] C.enneagrammus_191044_Mexico G...... C ...... T...... [450] C.hewardi_267097_Mandeville ...... [450]

195

C.maculatus_266527_Cayman_Brac ...... [450] C.maculatus_266550_Cayman_Brac ...... [450] C.marcanoi_193286_Valle_de_Baco ...... [450] C.marcanoi_193309_La_Lagunas ...... [450] C.haetianus_mylicus_102465_Cabral ...... [450] C.costatus_neiba_102922_La_Descubierta ...... [450] C.costatus_neiba_192428_Ca_Soleil ...... [450] C.costatus_neiba_266321_Rosa_de_La_Pierda ...... [450] C.costatus_neiba_266683_La_Zurza ...... [450] C.costatus_oreistes_192480_Puerto_Escondido ...... [450] C.costatus_oreistes_104408_Jacmel ...... [450] C.stenurus_rugosus_102371_El_Valle .A...... [450] C.stenurus_rugosus_103083_Nagua ...... [450] C.sepsoides_102369_Sabana_de_La_Mar ...... [450] C.warreni_172914_Hispaniola ...... [450] C.warreni_194521_Puerto_Plata ...... [450] C.stenurus_weinlandi_192405_Thomaseau ...... [450] C.stenurus_weinlandi_266286_El_Azul ...... [450] C.stenurus_weinlandi_266682_La_Zurza ...... [450] D.bilobatus_194940_Costa_Rica G...... C...... C...... [450] D.garridoi_193507_Cuba G...... T...... [450] D.nigropunctatus_191320_Cuba G...... T...... [450] D.nigropunctatus_191542_Cuba G...... T...... [450] D.nigropuntatus_191015_Cuba G...... T...... [450]

196

D.pleii_191574_Puerto_Rico G...... [450] O.apodus_194938 ...... [450] C.agasepsoides_160191_Canoa GGGAAATGAG TCTGGGAACA AACTCTTTAG GCGCTTTCG [489] C.curtissi_aporus_102610_La_Descubierta ...... [489] C.curtissi_aporus_102930_Barahona ...... [489] C.curtissi_aporus_194831_Bucan_Detwi ...... [489] C.badius_194964_Navassa_Island ...... [489] C.barbouri_161120_Quick_Step ...... [489] C.costatus_costatus_104113_Castillion ...... [489] C.costatus_costatus_192054_Abricots ...... [489] C.crusculus_101572_Content ...... [489] C.crusculus_103448_Knoxwood ...... [489] C.crusculus_103460_Jackson_Bay ...... C...... A [489] C.crusculus_161124_Salt_Gut ...... [489] C.crusculus_171607_Braeton ...... [489] C.crusculus_172442_Duncans ...... [489] C.crusculus_172465_Section ...... [489] C.curtissi_curtissi_194503_La_Descubierta ...... G...... [489] C.cyanochloris_194930_Costa_Rica ...G...... G...... [489] C.darlingtoni_161687_Cacique_Enriguillo ...... [489] C.curtissi_diastatus_194553_Mole_St._Nicolas ...... [489] C.duquesneyi_267952_Hellshire_Hills ...... [489] C.enneagrammus_191044_Mexico ...... C...... G...... [489] C.hewardi_267097_Mandeville ...... [489]

197

C.maculatus_266527_Cayman_Brac ...... [489] C.maculatus_266550_Cayman_Brac ...... [489] C.marcanoi_193286_Valle_de_Baco ...... [489] C.marcanoi_193309_La_Lagunas ...... [489] C.haetianus_mylicus_102465_Cabral ...... [489] C.costatus_neiba_102922_La_Descubierta ...... [489] C.costatus_neiba_192428_Ca_Soleil ...... [489] C.costatus_neiba_266321_Rosa_de_La_Pierda ...... [489] C.costatus_neiba_266683_La_Zurza ...... [489] C.costatus_oreistes_192480_Puerto_Escondido ...... [489] C.costatus_oreistes_104408_Jacmel ...... [489] C.stenurus_rugosus_102371_El_Valle ...... T .....C...... [489] C.stenurus_rugosus_103083_Nagua ...... [489] C.sepsoides_102369_Sabana_de_La_Mar ...... [489] C.warreni_172914_Hispaniola ...... [489] C.warreni_194521_Puerto_Plata ...... [489] C.stenurus_weinlandi_192405_Thomaseau ...... [489] C.stenurus_weinlandi_266286_El_Azul ...... [489] C.stenurus_weinlandi_266682_La_Zurza ...... [489] D.bilobatus_194940_Costa_Rica ...... G...... [489] D.garridoi_193507_Cuba ...... G...... [489] D.nigropunctatus_191320_Cuba ...... G...... [489] D.nigropunctatus_191542_Cuba ...... G...... [489] D.nigropuntatus_191015_Cuba ...... G...... [489]

198

D.pleii_191574_Puerto_Rico ...... G...... [489] O.apodus_194938 ...... G...... T..... [489]

199

ACADEMIC VITAE STEPHANIE L. DENNISON [email protected] 825 McKendimen Road Shamong, NJ 08088 (609) 268-9416

EDUCATION The Pennsylvania State University, May 2010 Eberly College of Science; Schreyer Honors College Bachelor of Science in Biology – Vertebrate Physiology option; Minor in Dance and Psychology

THESIS TITLE Molecular Phylogeny and Biogeography of Neotropical Lizards of the Family Diploglossidae

THESIS SUPERVISOR S. Blair Hedges, Professor of Biology Daniel J. Cosgrove, Professor of Biology

RESEARCH EXPERIENCE Mueller Lab: Department of Biology (Prof. S. Blair Hedges), University Park, PA Schreyer Scholar – Researcher January 2006-present Self-directed honors research project extracted DNA from limited tissue samples of 180 lizard species amplified and obtained DNA sequence for five genes of each species via PCR determined the molecular phylogeny and evolutionary history of these lizard species Mueller Lab: Department of Biology (Prof. S. Blair Hedges), University Park, PA Research Assistant January 2006-present Assisted researcher Performed laboratory procedures, recorded and analyzed data collected

WORK EXPERIENCE Dr. S. Blair Hedges Evolutionary Lab Research assistant May 2008-present Assisted researcher Performed laboratory procedures, recorded and analyzed data collected Virtua Memorial Hospital Patient Escort May 2007-present Transported patients of all severity of illness from room to testing area Accountable for utmost hospital standards in patient privacy and satisfaction The Pennsylvania State University

Biology Teaching Assistant May 2008-Dec. 2008 constructed lesson plans for a summer and fall introductory biology course taught biological concepts to twenty five incoming freshman mastered and demonstrated innumerable laboratory techniques Dr. Dazen and Dr. Cadieux Family Dentistry Secretarial Assistant, Shadow Jun. 2005-Aug. 2005 opened and closed dental office, checked patients in and out responsible for organizing files, confirming insurance, billing, and answering phone gained exposure to intimate details of the dental profession

COMMUNITY SERVICE Habitat for Humanity May 2004- Jan.2010 organized and distribute food in food banks in Virginia and Massachusetts constructed homes in Virginia, Massachusetts, and New Jersey active member, and teen leader for Lord of Life Lutheran Church Habitat for Humanity Virtua Memorial Hospital May 2007- Jan. 2010 transported patients of all severity of illness from room to testing area attended to call bells for basic patient needs Center for Volunteers in Medicine Sept.2008- Nov. 2008 helped establish new facility for free medical and dental clinic in State College, PA responsible for greeting patients and filing patient files

HONORS AND ACTIVITIES PSU Dance Program, PSU competition company member, dancer’s repertory member Vole The PSU Ballet Club, company member, special performance company Project Haiti, active member, missionary team member Women in Science and Engineering, active member, stay over committee member Penn State Student Leadership and Achievement Scholarship (2010) Phi Kappa Phi Honor Society (top 10% of Penn State Eberly College of Science) (2010) Golden Key International Honor Society (top 8% of Penn State class) (2009) Schreyer Scholar (2008) Edward C. Hammond Memorial Scholarship for excellence in biology (2008) Dean’s List six out of eight semesters (3.5 or greater on a 4.0 scale) Air Force Officers Wives’ Outstanding Academic Achievement Scholarship (2006)