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Supplementary Table 1 Genes involved in adhesion/migration up- or downregulated in emergent vs. young keratinocytes t-test Genbank Name Alternate name Full name Function 0.010164129 up NM_020403 PCDH9 protocadherin 9 Localized to synaptic junctions 5.770558409 down NM_018937 PCDHB3 PCDH-BETA3 protocadherin beta 3 Cell-cell adhesion Protocadherin involved in polarization and directed 0.020029928 up AK054903 moderately similar to mouse fat 1 cadherin migration Localizes to the baso-lateral membrane of polarized 0.024951525 up NM_018836 AJAP1 MOT8; SHREW1 adherens junction associated protein 1 epithelial cells plakophilin 1 (ectodermal dysplasia/skin Localizes to desmosomes. Links cadherin to 5.728123444 down NM_000299 PKP1 fragility syndrome). intermediate filament. Also localizes to nuclei 7.667061664 down NM_020435 GJC2 Cx47; GJA12; CX46.6 gap junction protein, gamma2 Component of gap junctions 5.670256384 down NM_003829 MPDZ MUPP1 multiple PDZ domain protein. Associated with tight junctions EIF3A; p27BBP; translation initiation factor 6. Also integrin- Translation initiation factor. Also a component of 7.515596763 down NM_002212 EIF6 ITGB4BP beta4 subunit hemidesmosomes 5.448743723 down NM_002210 ITGAV CD51; MSK8, VNRA integrin, alpha V (vitronectin receptor) Adhesion to extracellular matrix 6.265892046 down NM_002414 CD99 Cell adhesion glycoprotein 5.90671464 down NM_003247 THBS2 TSP2 thrombospondin 2 Cell-cell and cell-matrix interactions. Migration. Component of the basal membrane. Component of 0.016774709 up NM_005560 LAMA5 laminin alpha 5 the anchoring complex connecting keratinocytes to the underlying dermis. Secreted by keratinocytes fibronectin type III domain containing 3B; 6.54405465 down NM_022763 FNDC3B FAD104; PRO4979 factor for adipocyte differentiation 104 ADMP-1; ADAMTS-2; ADAM metallopeptidase with Degradation of aggrecan and brevican, two 5.541533027 down NM_005099 ADAMTS4 ADAMTS-4 thrombospondin type 1 motif, 4 components of the extracellular matrix protein tyrosine phosphatase, non-receptor 5.322172089 down NM_015466 PTPN23 HDPTP; HD-PTP Involved in trafficking of endosomes type 23 Genes involved in cytoskeleton structure and dynamics up- or downregulated in emergent vs. young keratinocyt t-test Genbank Name Alternate name Full name Function Component of microtubules. A nuclear form exists, 0.013092094 up NM_001069 TUBB2A TUBB; TUBB2 tubulin beta 2A that binds Notch 0.002168978 up XM_067193 similar to microtubule-associated proteine 6 0.00520902 up NM_005909 MAP1B MAP5; FUTSCH microtubule-associated protein 1B Microtubule assembly myosin, light chain 6B, alkali, smooth 0.013093579 up NM_002475 MYL6B MLC1SA ATPase motor protein muscle and non-muscle PCH family. Cytoskeletal-associated protein. proline-serine-threonine phosphatase 8.832965442 down NM_024430 PSTPIP2 MAYP Regulates F-actin bundling. Enhances filopodia and interacting protein 2 motility 7.381029035 down NM_004411 DYNC1I1 DNCI1, DNCIC1 dynein, cytoplasmic 1, intermediate chain 1 Binds to dynein Genes involved in senescence/oxidative stress/DNA damage up- or downregulated in emergent vs. young keratinoc t-test Genbank Name Alternate name Full name Function YTH domain family, member 2; high glucose 0.006297251 up NM_016258 YTHDF2 HGRG8, NY-REN-2 regulated protein 8 SUMO2/3 conjugate with high molecular proteins 6.538781448 down NM_006936 SUMO3 SMT3 suppressor of mif two 3 homolog 3 following stress. Reduction of methionine sulfoxide to methionine. 0.020843235 up NM_012331 MSRA methionine sulfoxide reductase A Repair of oxidative damage to proteins NADH dehydrogenase ubiquinone 1 alpha 0.024998491 up NM_175614 NDUFA11 B14.7 complex I of the respiratoiry chain subcomplex 11 Orphan nuclear receptor. Stimulated by oxidative nuclear receptor subfamily 2, group C, 0.007309126 up NM_003298 NR2C2 TR4; TAK1; TR2R1 stress trhough FOXO3a. Apoptosis modulator via member 2 Bcl2 regulation Protein tyrosine phosphatase family (PTP). Role in proteine tyrosine phosphatase, non-receptor 0.000522895 up NM_002833 PTPN9 MEG2; PTPMEG2 secretory pathway. Modulator of insulin-dependent type 9 FOXO1 nuclear exclusion GTPase, retrograde traffic between Golgi and ER. 0.008673079 up NM_031296 RAB33B RAB33B, member of RAS oncogen family Golgi resident. Interacts with Atg16L and modulates autophagosome formation Nedd4: E3 ubiquitine ligase. Also binds and inhibit Itch, a Nedd4 related E3. Nedd4 are involved in 0.011705083 up NM_014664 N4BP1 Nedd4 binding protein 1 lysosomal and proteasomal degradation. Itch controls the stabilitty of p63 and p73 excision-repair cross-complementing rodent XPB; BTF2; GTF2H, repai deficiency, complementation group 3 ATP-dependent helicase that functions in nucleotide 8.169165982 down NM_000122 ERCC3 RAD25; TFIIH (xeroderma pigmentosum group B excision repair. Also the 89 kda subunit of TFIIH complementing) Genes involved in cell cycle progression or cell death up- or downregulated in emergent vs. young keratinocyte t-test Genbank Name Alternate name Full name Function programmed cell death 4 -neoplastic Localized to the nucleus in proliferating cells. Role in 6.747502842 down NM_014456 PDCD4 transformation inhibitor) apoptosis but not defined Phosphatase whose substrates are: HDAC3, NFkB 0.003394879 up NM_005134 PPP4R1 PP4R1; PP4(Rmeg) protein phosphatase 4, regulatory subunit 1 p65. Pro-apoptotic. DNA damage response. Centrosome maturation C9; DD1; DDH; H-37; aldo-keto reductase family 1, member C1; MBAB; HAKCR; 2- 0.009699459 up NM_001353 AKR1C1 dihydrodiol dehydrogenase 1, 20-alpha (3- Degrades progesterone ALPHA-HDS; 20-ALPHA- alpha)-hydroxysteroid dehydrogenase HDS Transcription factor of the E2F family. Repressor? 0.007374601 up NM_001952 E2F6 E2F6 Anti-apoptotic via BRCA1 inhibition, couteraction on E2F1 activity Belongs to argonaute family. Involved in chromatin 5.262452266 down NM_152431 PIWIL4 piwi-like 4. remodelling by inducing histone methylation. Induces methylation at the p16 locus Component of the mitotic spindle assembly 0.018466307 up NM_006341 MAD2L2 REV7; MAD2B MAD2 mitotic arrest deficient-like 2 checlpoint that prevents anaphase Structurally divergent member of BMP family. Limits 0.019704145 up NM_001201 BMP3 BMP-3A bone morphogenetic protein 3 BMP and TGFbeta1 signalling Transmenbrane protein. Involved in TGFbeta/EGF 12.61036282 down NM_014184 CNIH4 HSPC163 cornichon homolog 4 secretion VEGF C and D receptor. Involved in 0.020256547 up BC027302 FLT4 PCL; VEGFR3 fms-related tyrosine kinase 4 lymphangiogenesis and maintenance of the lymphatic endothelium Genes involved in cancer up- or downregulated in emergent vs. young keratinocytes t-test Genbank Name Alternate name Full name Function Type II membrane protein that catalyzes the transfert ST3N; SIAT6; beta-galactoside alpha-2,3-sialyltransferase 0.002253894 up NM_006279 ST3GAL3 of sialic acid to galactose-containning substrates. ST3GALIII 3 Localizes to Golgi. Monooxygenase which catalyzes the degradation of cytochrome p450, family 24, subfamily A, 0.008105121 up NM_000782 CYP24A1 CP24; CYP24 1,25-dihydroxyvitamin D3, the active form of vitamin polypeptide 1 D3. Localizes to mitochondria 5.311795768 down S69025 HOX A1 homeobox A1 Transcription factor Zinc-finger transcription factor. Role in epithelial and germ cell development by modulating the balance 5.385202954 down NM_021220 OVOL2 movol2; movo2 ovo-like 2 between proliferation and differentiation of progenitor cells Histone methyl transferase with a chromodomain and a SET domain. Moves to centromeres during mitosis. 0.01994811 up NM_003173 SUV39H1 MG44; KMT1A; SUV39H suppressor of variegation 3-9 homolog 1 Required for pericentric heterochromatin organization, chromosome segregation, mitotic progression 0.019471283 up NM_020892 DTX2 RNF58 deltex homolog 2 E3 ubiquitin ligase. Notch signalling RNA-binding protein. Splicing factor. Expression 0.018608707 up NM_002516 NOVA2 ANOVA; NOVA3 neuro-oncological ventral antigen 2 restricted to astrocytes. GGF; HGL; HRG; NDF; ErbB ligand. Involved in growth and differentiation of 0.02136094 up NM_013956 NRG1 ARIA; GGF2; HRG1; neuregulin1 several cell types, including epithelial cells HRGA; SMDF Stable secreted protein with a trefoil motif. May 10.26533153 down NM_005423 TFF2 SP; SML1 trefoil factor 2/spamolytic protein1 protect mucosa PSGR; PSGR2; olfactory receptor, family 51, subfamily E, 9.639400736 down NM_030774 OR51E2 prostate-specific G protein-coupled receptor OR52A2; OR51E3P member 2.
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