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(Nematoda: Aphelenchoididae) from Iran J. Crop Prot. 2017, 6 (1): 125-143______________________________________________________ Research Article Description of some known species of the genus Aphelenchoides Fisher, 1984 (Nematoda: Aphelenchoididae) from Iran Yaser Adeldoost, Ramin Heydari*, Mehrab Esmaeili and Esmaeil Miraeiz Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. Abstract: Twelve species of the genus Aphelenchoides were collected and identified based on morphological and morphometric characters from different regions in North, South and Western Iran. Among collected species i. e. A. centralis, A. cibolensis, A. cyrtus, A. hamatus, A. helicus, A. huntensis, A. limberi, A. obtusicaudatus, A. sprophilus, A. sacchari, A. spicomucronatus and A.tuzeti, three species namely A. cibolensis, A. hamatus and A. saprophilus are new records from Iran. Description, measurements, line drawings and microscopic photographs for Iranian population of these species are provided. Aphelenchoides helicus that was previously reported without description, is described and illustrated as well. Keywords: morphology, morphometric, new record Introduction12 To date, 37 species of the genus have been reported from different hosts and localities in The genus Aphelenchoides Fisher, 1984 belongs Iran (Ghaderi et al., 2012; Esmaeili et al., to the family Aphelenchoididae Skarbilovich, 2016a,b, 2017a,b; Golhasan et al., 2016a,b; 1947. It comprises over 150 nominal species Miraeiz et al., 2017), for some of them there is that inhabit soil and decaying plant material and no morphological data. Recently, A. huntensis are also associated with the fungi colonizing Esmaeili, Fang, Li and Heydari, 2016, A. fuchsi bark (Hunt, 1993, 2008). Most species of Esmaeili Heydari, Ziaei and Gu, 2016, A. Aphelenchoides are fungivorous (Kanzaki and eldaricus Esmaeili, Heydari, Golhasan and Giblin-Davis 2012), thirteen species have been Kanzaki, 2017 collected in association with reported as plant-parasitic in a wide variety of pine trees, and A. iranicus Golhasan, Heydari, plants (Sánchez-Monge et al., 2015). Three Alvarez-Ortega, Esmaeili, Castillo and species of the genus namely A. besseyi Christie, Palomares-Rius, 2016 and A. paraxui Esmaeili, 1942, A. fragariae (Ritzema Bos, 1890) Heydari, Fang and Li, 2017 in association with Christie, 1932 and A. ritzemabosi (Schwartz, oak trees, were described from Iran. In the 1911) Steiner and Buhrer, 1932, that are present paper, we have provided economically important, causing losses in a morphometrics, illustrations and microscopic range of agricultural and horticultural crops photographs for four species of Aphelenchoides have been extensively studied (Duncan and including three new records from Iran and a Moens, 2013). previously reported species. Materials and Methods Handling Editor: Zahra Tanhamaafi ________________________________ * Corresponding author, e-mail: [email protected] More than 100 soil, root and bark samples were Received: 24 April 2016, Accepted: 1 April 2017 randomly collected from different fields, Published online: 11 June 2017 125 Description of Aphelenchoides species from Iran ______________________________________ J. Crop Prot. orchards, forests, and grasslands in Fars, (Figs 1 and 2; Table 2) Golestan and Kermanshah provinces during Female. Body slender and slightly curved 2013-2014. The Whitehead tray (Whitehead ventrally upon fixation. Cuticle with distinct and Hemming, 1965) was used to extract annuli, 1.0-1.2 µm in mid-body. Lateral field nematodes. Specimens were heat-killed by with three lines, 20-23% of corresponding body adding hot 4% formaldehyde solution, width. Lip region rounded, offset, ca 3.0-4.0 μm processed to dehydrated glycerin according to high and 5.0-5.5 μm wide. Stylet with small De Grisse (1969) and observed under light basal swellings, procorpus cylindrical. Median microscope (LM). Measurements and drawings bulb strongly developed, almost spherical, with were made with a Nikon-E200 light microscope conspicuous valve situated more or less equipped with a drawing tube and were redrawn centrally. Pharyngo-intestinal junction using CorelDraw® software version 17. Species immediately posterior to metacorpus. Excretory were identified based on the valid key (Shahina, pore with slight variation in position, at level of 1996) and related original descriptions. base of median bulb to nerve ring. Hemizonid not observed. Nerve ring situated at ca one-half Results metacarpus length posterior to it. Ovary outstretched anteriorly, developing oocytes in Twelve species belonging to the genus single row. Spermatheca oval, sperm present in Aphelenchoides (see Table 1) were collected some individuals. Vagina obliquely transverse and identified. Aphelenchoides cibolensis, A. one-third body width. Vulva transverse, with hamatus and A. saprophilus, as new records for slightly raised lips. Post-uterine sac well Iran nematode fauna, and one previously developed, extending for about one-sixth of reported species; A. helicus are described and vulva to anus distance. Rectum and anus visible. illustrated. Tail truncated, dorsally almost angular as it forms a single sharply pointed ventral mucro. Aphelenchoides cibolensis Riffle, 1970 Male: Not found. Table 1 Aphelenchoides species recovered from rhizosphere soil, root and bark samples of plants in the present study. Species Locality (provinces) Associated plants A. centralis Fars, Golestan & Kermanshah Apple & Grapevine A. cibolensis* Fars & Kermanshah Apricot & Palm A. cyrtus Kermanshah Grapevine A. hamatus* Kermanshah Grapevine A. helicus Golestan Tomato A. huntensis Kermanshah Pine tree A. limberi Fars & Kermanshah Clover, Ficus & Olive A. obtusicaudatus Fars Apple A. saprophilus* Fars & Golestan Alfalfa, Forest tree & Olive A. sacchari Fars & Golestan Apple A. spicomucronatus Fars, Golestan & Kermanshah Apple, Cherry & Oak A. tuzeti Fars & Kermanshah Grapevine * New records for Iranian nematode fauna. 126 Adeldoost et al. ____________________________________________________ J. Crop Prot. (2017) Vol. 6 (1) Figure 1 Aphelenchoides cibolensis. A: Vulva-body end; B: Female reproductive system; C: Anterior end of female; D: Female pharyngeal region. 127 Description of Aphelenchoides species from Iran ______________________________________ J. Crop Prot. Figure 2 Aphelenchoides cibolensis. A: Entire body; B: Anterior end; C: Anterior end showing excretory pore (arrowhead); D: Metacorpus region showing excretory pore (arrowhead); E: Vulva region; F: Pharyngeal gland region; G: Lateral field at mid body; H: Vulva-body end; I-L: Various shapes of tail. (Scale - bars 10 µm, except A 40 µm & H 20 µm). 128 Adeldoost et al. ____________________________________________________ J. Crop Prot. (2017) Vol. 6 (1) Table 2 Morphometric data of females of Aphelenchoides cibolensis compared with type population. Fars province population Riffle (1970) Characters1 Female Female n n 10 10 L L 374 ± 4.9 (364-382) 410 (360-460) a a 24.2 ± 0.9 (22.7-25.7) 26 (23-29) b b 6.5 ± 0.3 (6.2-7.1) 8 (7.3-8.9) b' 3 ± 0.1 (2.9-3.2) - c c 17.3 ± 1.4 (15.5-19.2) 15 (13-16) c' 3 ± 0.3 (2.5-3.4) - V or T (%) 70.3 ± 1 (68.6-72.1) 70 (69-71) Stylet 9.6 ± 0.4 (9-10) 10 (9-11) m m 42.8 ± 3.1 (40-50) - End of glands 125 ± 2.5 (120-130) - Overlapping 67 ± 2.5 (63-72) - Median bulb 45.8 ± 1.1 (44-48) - Pharynx 58± 2.2 (53-60) - Anterior end to Nerve ring 54.9 ± 1.5 (53-57) - Anterior end to Excretory pore 49.9 ± 1.6 (48-53) - Body width (BW) 15.5 ± 0.6 (14.5-16.5) - Post-uterine sac (PUS) length 11.9 ± 1.9 (10-15) - PUS/BW 0.8 ± 0.1 (0.6-1) - PUS/ Vulva-anus (%) 13.6 ± 2.4 (11-17.1) - Vulva–anus distance 87.9 ± 2.8 (82.5-91) - Anal BW 7.4 ± 0.3 (7-8) - Tail length 21.8 ± 1.8 (19-24) - 1 All measurements are given in micrometer, except V, T and PUS which are in percent. Remarks first time. This species is morphologically close Female of Aphelenchoides cibolensis was to A. dactylocercus Hooper, 1958, A. originally described from New Mexico in the parabicaudatus Shavrov, 1967, A. sacchari rootlets of juniper tree (Juniperus deppeana L.) Hooper, 1958 and A. trivialis Franklin & by Riffle (1970). Later, the male of this species Siddiqi, 1963. It differs from A. dactylocerus by was described by Hooper and Myers (1971). shorter tail length (19-24 vs 38-40 µm) and Morphological and morphometric characters of shape of tail terminus (flattened vs rounded). the Iranian population of A. cibolensis fit well Aphelenchoides cibolensis is distinguished from with the original description. This population A. parabicaudatus by having three lines in was collected from the rhizosphere of an apricot lateral field vs four, position of excretory pore tree in Nurabad (Fars province) also from the (at level of base of median bulb to nerve ring vs rhizosphere of palm tree in Ghasr-e Shirin posterior to nerve ring) and higher V ratio (Kermanshah province), and is reported for the (68.6-72.1 vs 61-64). Rashid et al. (1986) 129 Description of Aphelenchoides species from Iran ______________________________________ J. Crop Prot. described a population of A. parabicaudatus Rectum and anus visible. Tail conical, from Brazil which the lateral field of specimens tapering to a rounded point bearing a small was not so clear and the authors mentioned the and simple mucro. number of incisures with probability as three. It Males. Body slender, cylindrical, J-shaped differs from A. sacchari by shorter body length when heat-relaxed. Anterior region and cuticle (360-380 vs 654-810 μm), shorter post-uterine similar to female. Testis single, anteriorly sac (10-15 vs 77-120 μm) and shape of tail outstretched. Spicules arcuate, relatively long, terminus (flattened vs rounded). It differs from apex and rostrum rounded and well developed, A. trivialis by position of excretory pore the end of the dorsal limb is clearly curved (anterior to nerve ring vs posterior) and shape of ventrally like a hook. Gubernaculum absent. tail terminus (flattened vs rounded). Tail conical, bearing a short sharp mucro ca The two populations of A. cibolensis 1.6-2.2 μm long.
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