Sheep Anti-Human Neutrophil Elastase Polyclonal Antibody (DPAB2237SH) This Product Is for Research Use Only and Is Not Intended for Diagnostic Use

Sheep anti-Human Neutrophil Elastase Polyclonal antibody (DPAB2237SH) This product is for research use only and is not intended for diagnostic use.

PRODUCT INFORMATION

Antigen Description Elastasesform a subfamily of serine proteases that hydrolyze many proteins in additionto elastin. Humans have six elastase genes which encode the structurallysimilar proteins. The product of this gene hydrolyzes proteins withinspecialized neutrophil lysosomes, called azurophil granules, as well asproteins of the extracellular matrix following the protein"s release fromactivated neutrophils. The enzyme may play a role in degenerative andinflammatory diseases by its proteolysis of collagen-IV and elastin of theextracellular matrix. This protein degrades the outer membrane protein A(OmpA) of E. coli as well as the virulence factors of such bacteria asShigella, Salmonella and Yersinia. Mutations in this gene are associated withcyclic neutropenia and severe congenital neutropenia (SCN). This gene isclustered with other serine protease gene family members, azurocidin 1 andproteinase 3 genes, at chromosome 19pter. All 3 genes are expressedcoordinately and their protein products are packaged together into azurophilgranules during neutrophil differentiation.

Specificity Prior toconjugation, this antibody was specific for neutrophil elastase as demonstratedby immunoelectrophoresis and ELISA.

Immunogen Neutrophilelastase (active-site blocked) prepared from human sputum.

Source/Host Sheep

Species Reactivity Human

Conjugate HRP

Buffer A buffered stabilizer solution containing 50% glycerol (v/v).

Preservative None

Storage Store between –10°Cand –20°C. Product will become viscous but willnot freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allowproduct to warm to room temperature and gently mix before use. Avoid exposureto sodium azide as this is an i

GENE INFORMATION

45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected]

Official Symbol ELANE

Synonyms ELANE; elastase,neutrophil expressed; GE; NE; HLE; HNE; ELA2; SCN1; PMN-E; neutrophilelastase; elastase-2; medullasin; PMN elastase; leukocyte elastase; elastase2, neutrophil; human leukocyte elastase; polymorphonuclear elastase; bonemarrow serine protease; granulocyte-derived elastase; EC=3.4.21.37; EC 3.4.21

Entrez Gene ID 1991

Protein Refseq NP_001963

UniProt ID P08246

Chromosome Location 19p13.3

Pathway C-MYB transcriptionfactor network; Systemic lupus erythematosus; Transcriptional misregulation; amb2Integrin signaling

Function bacterial cellsurface binding; cytokine binding; endopeptidase activity; heparin binding;peptidase activity; protein binding; serine-type endopeptidase activity

45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected]