Stanley Miller Papers
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Excited State Dynamics of Isocytosine; a Hybrid Case of Canonical Nucleobase Photodynamics
The Journal of Physical Chemistry Letters This document is confidential and is proprietary to the American Chemical Society and its authors. Do not copy or disclose without written permission. If you have received this item in error, notify the sender and delete all copies. Excited State Dynamics of Isocytosine; a Hybrid Case of Canonical Nucleobase Photodynamics Journal: The Journal of Physical Chemistry Letters Manuscript ID jz-2017-020322.R1 Manuscript Type: Letter Date Submitted by the Author: n/a Complete List of Authors: Berenbeim, Jacob; UC Santa Barbara, Chemistry and Biochemistry Boldissar, Samuel; UCSB, Department of Chemistry Siouri, Faady; UCSB, Department of Chemistry Gate, Gregory; UCSB, Department of Chemistry Haggmark, Michael; UC Santa Barbara, Chemistry and Biochemistry Aboulache, Briana; University of California Santa Barbara Cohen, Trevor; UC Santa Barbara, Chemistry and Biochemistry De Vries, Mattanjah; UCSB, Department of Chemistry ACS Paragon Plus Environment Page 1 of 12 The Journal of Physical Chemistry Letters 1 2 3 4 Excited State Dynamics of Isocytosine; A Hybrid Case of Canonical 5 Nucleobase Photodynamics 6 7 Jacob A. Berenbeim, Samuel Boldissar, Faady M. Siouri, Gregory Gate, Michael R. 8 Haggmark, Briana Aboulache, Trevor Cohen, and Mattanjah S. de Vries* 9 10 Department of Chemistry and Biochemistry, University of California Santa 11 12 Barabara, CA 93106-9510 13 *E-mail: [email protected] 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 1 ACS Paragon Plus Environment The Journal of Physical Chemistry Letters Page 2 of 12 1 2 3 Abstract 4 5 We present resonant two-photon ionization (R2PI) spectra of isocytosine (isoC) and pump-probe 6 results on two of its tautomers. -
The Identification of Olefins As Thiocyanates
THE IDENTIFICATION OF OLEFI NS AS THIOCYANATES 1 .. .SEP 2"/ i 938 THE IDENTIFICATION OF OLEFINS AS THIOCYANAT ES/ ( ' By George A. Dysinger I\ Bachelor of Science Oklahoma Agricultural and Mechanical College Stillwater, Oklahoma 1937 Submitted to the Department of Chemistry Oklahoma Agricultural and Mechanical College In partial fulfillme.nt of the requirements for the Degree of MASTER OF SCIENCE 1938 . ... .. '.'' .. ~ . ..... .. • • • • • • • • J • : ... ·:· .· ~- . .. ,. r f • • • - • • • • •• J • •• ; • • • ii !)fp 27 1938 APPROVED: HeadOttinm~- of : e Department of Onemistry ~~~e~ 108550 iii ACKNOWLEDGEMENT The author wishes to expres.s his sincere appreciation to Dr. o. c. Dermer under whose direction and with whose help this work has been done. He also wishes to acknowledge the assistance rendered by Everett L. Ada.ms in supplying apparatus and chemicals. iv TABLE OF CONTENTS Page Introduotion • . .. • . • • • 1 Historical Review. • . • • . • . 2 Experimental . • • • • . • . • . • • 6 Discussion of Results . .. ao Summary • • • • . • • • 22 Bibliography • • • • • • • • • • • • • • • • • • 23 Autobiography • • • • • . • • • • • • • • • . • 24 1 INTRODUCTION At present, the identification of low boiling unsatu rated hydrocarbons which yield only liquid addition products with the halogens and hydrogen halides is often a matter of considerable difficulty. This f'aot is sufficient reason for the study here described. In some cases, espeoially among terpen.es, the compounds formed by adding NOCl, 1203 , or »2o4 at one or more of the double bonds have been used as derivatives but these are i nconvenient to make,. of un certain composition, and decidedly unstable! This work con- '\, sists of (l) a'ttempts to find a convenient samll scale meth- od for adding (S0N)2 to olef1ns, (2) the determinations of the melting points of the derivatives, and (3) quantitatiye analysis of derivatives to prove their structure and purity. -
A Production of Amino Acids Under Possible Primitive Earth Conditions
A Production of Amino Acids Under Possible Primitive Earth Conditions Stanley L. Miller Methods and Logic - 2/24/15 Outline for today’s class • The origin of life • Stanley L. Miller and Harold Urey • Background • Landmark Paper • Landmark Experiment • Subsequent Studies There are many theories regarding the origin of life • Theory of spontaneous generation: living organisms can arise suddenly and spontaneously from any kind of non-living matter • Aristotle, ancient Egyptians • Popular until 1600s when it was disproved due to various experiments • Fransisco Redi (1665) http://www.tutorvista.com/content/b iology/biology-iii/origin-life/origin- • Louis Pasteur (1864) life-theories.php# http://bekarice.com/college-spontaneous-generation/ There are many theories regarding the origin of life • Cozmozoic theory (parpermia): life reached Earth from other heavenly bodies such as meteorites, in the form of highly resistance spores of some organisms • Richter (1865) • Arrhenius (1908) • Overall lack of evidence Wikipedia • Living matter cannot survive the extreme cold, dryness and ultra-violet radiation from the sun required to be crossed for reaching the earth. There are many theories regarding the origin of life • Theory of chemical evolution: Origin of life on earth is the result of a slow and gradual process of chemical evolution that probably occurred about 4 billion years ago • Oparin (1923) • Haldone (1928) • Early Earth atmosphere (mixture of gases and solar radiation/lightning) • Miller-Urey Experiment Stanley L. Miller - Biography Born: 1930 in Oakland, CA Died: 2007 in San Diego, CA High school nickname: “a chem whiz” BS: UC Berkley - 1951 PhD: University of Chicago – 1954 (advisor: Harold Urey) California Institute of Technology Columbia University UC San Diego (1960-2007) National Academy of Sciences Landmark Paper: (1953) Production of amino acids under possible primitive earth conditions". -
Stanley L. Miller 1930–2007
Stanley L. Miller 1930–2007 A Biographical Memoir by Jeffrey L. Bada and Antonio Lazcano ©2012 National Academy of Sciences. Any opinions expressed in this memoir are those of the authors and do not necessarily reflect the views of the National Academy of Sciences. STANLEY L. MILLER March 7, 1930–May 20, 2007 Elected to the NAS, 1973 Stanley l. Miller, who was considered to be the father of prebiotic chemistry—the synthetic organic chemistry that takes place under natural conditions in geocosmochem- ical environments—passed away on May 20, 2007, at age 77 after a lengthy illness. Stanley was known worldwide for his 1950s demonstration of the prebiotic synthesis of organic compounds, such as amino acids, under simu- lated primitive Earth conditions in the context of the origin of life. On May 15, 1953, while Miller was a graduate student of Harold C. Urey at the University of Chicago, he published a short paper in Science on the synthesis of Stanley Miller Papers, the Mandeville of negavtive in The Register From at California University of the Geisel Library, Special Collection Library at 5. file 163, San Diego; MSS 642, box amino acids under simulated early Earth conditions. This paper and the experiment it described had a tremendous impact and immediately transformed the study of the By Jeffrey L. Bada origin of life into a respectable field of inquiry. and Antonio Lazcano Stanley Lloyd Miller was born in March 7, 1930, in Oakland, California, the second child (the first was his brother, Donald) of Nathan and Edith Miller, descendants of Jewish immigrants from the eastern European countries of Belarus and Latvia. -
Columbia University Task Force on Climate: Report
COLUMBIA UNIVERSITY TASK FORCE ON CLIMATE: REPORT Delivered to President Bollinger December 1, 2019 UNIVERSITY TASK FORCE ON CLIMATE FALL 2019 Contents Preface—University Task Force Process of Engagement ....................................................................................................................... 3 Executive Summary: Principles of a Climate School .............................................................................................................................. 4 Introduction: The Climate Challenge ..................................................................................................................................................... 6 The Columbia University Response ....................................................................................................................................................... 7 Columbia’s Strengths ........................................................................................................................................................................ 7 Columbia’s Limitations ...................................................................................................................................................................... 8 Why a School? ................................................................................................................................................................................... 9 A Columbia Climate School ................................................................................................................................................................. -
5-Fluorocytosine/Isocytosine Monohydrate. the First Example of Isomorphic and Isostructural Co-Crystal of Pyrimidine Nucleobases
crystals Article 5-Fluorocytosine/Isocytosine Monohydrate. The First Example of Isomorphic and Isostructural Co-Crystal of Pyrimidine Nucleobases Gustavo Portalone Department of Chemistry, ‘Sapienza’ University of Rome, 00185 Rome, Italy; [email protected]; Tel.: +396-4991-3715 Received: 1 October 2020; Accepted: 2 November 2020; Published: 3 November 2020 Abstract: To date, despite the crucial role played by cytosine, uracil, and thymine in the DNA/RNA replication process, no examples showing isomorphic and isostructural behavior among binary co-crystals of natural or modified pyrimidine nucleobases have been so far reported in the literature. In view of the relevance of biochemical and pharmaceutical compounds such as pyrimidine nucleobases and their 5-fluoroderivatives, co-crystals of the molecular complex formed by 5-fluorocytosine and isocytosine monohydrate, C H FN O C H N O H O, have been synthesized 4 4 3 · 4 5 3 · 2 by a reaction between 5-fluorocytosine and isocytosine. They represent the first example of isomorphic and isostructural binary co-crystals of pyrimidine nucleobases, as X-ray diffraction analysis shows structural similarities in the solid-state organization of molecules with that of the (1:1) 5-fluorocytosine/5-fluoroisocytosine monohydrate molecular complex, which differs solely in the H/F substitution at the C5 position of isocytosine. Molecules of 5-fluorocytosine and isocytosine are present in the crystal as 1H and 3H-ketoamino tautomers, respectively. They form almost coplanar WC base pairs through nucleobase-to-nucleobase DAA/ADD hydrogen bonding interactions, demonstrating that complementary binding enables the crystallization of specific tautomers. Additional peripheral hydrogen bonds involving all available H atom donor and acceptor sites of the water molecule give a three-dimensional polymeric structure. -
Durham Research Online
Durham Research Online Deposited in DRO: 04 January 2019 Version of attached le: Published Version Peer-review status of attached le: Peer-reviewed Citation for published item: Pohl, Radek and Socha, Ond§rejand Slav¡§cek,Petr and S¡ala,Michal§ and Hodgkinson, Paul and Dra§c¡nsk¡y, Martin (2018) 'Proton transfer in guaninecytosine base pair analogues studied by NMR spectroscopy and PIMD simulations.', Faraday discussions., 212 . pp. 331-344. Further information on publisher's website: https://doi.org/10.1039/C8FD00070K Publisher's copyright statement: This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Additional information: Use policy The full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-prot purposes provided that: • a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders. Please consult the full DRO policy for further details. Durham University Library, Stockton Road, Durham DH1 3LY, United Kingdom Tel : +44 (0)191 334 3042 | Fax : +44 (0)191 334 2971 https://dro.dur.ac.uk Faraday Discussions Cite this: Faraday Discuss.,2018,212,331 View Article Online PAPER View Journal | View Issue Proton transfer in guanine–cytosine base pair analogues studied by NMR spectroscopy and PIMD simulations† a a b a Radek Pohl, Ondˇrej Socha, Petr Slav´ıcek,ˇ Michal Sˇala,´ c a Paul Hodgkinson and Martin Dracˇ´ınsky´ * Received 28th March 2018, Accepted 1st May 2018 DOI: 10.1039/c8fd00070k It has been hypothesised that proton tunnelling between paired nucleobases significantly enhances the formation of rare tautomeric forms and hence leads to errors in DNA Creative Commons Attribution-NonCommercial 3.0 Unported Licence. -
207650 / Prebiotic Synthesis of Diaminopyrimidine And
J Mol Evol (1996) 43:543-550 )o. =MOLECUI.AR 207650 NASAJCR.--- ---- 0 Swinga-Veriq New Yo_ Inc, 1996 / /. // / f ..4 " 2 / .o :/ Prebiotic Synthesis of Diaminopyrimidine and Thiocytosine Michael P. Robertson,* Matthew Levy, Stanley L. Miller Deparunent of Chemistry and Biochemistry. University of California. San Diego. La Jolla, CA 92093-0317. USA Received: 12 December 1995 / Accepted: 29 June 1996 Abstract. The reaction of guanidine hydrochloride Key words: Pyrimidine synthesis -- Cyanoacetalde- with cyanoacetaldehyde gives high yields (40-85%) of hyde -- Guanidine -- Thiourea--Drying lagoons 2,4-diaminopyrimidine under the concentrated condi- tions of a drying lagoon model of prebiotic synthesis, in contrast to the low yields previously obtained under more dilute conditions. The prebiotic source of cyano- Introduction acetaldehyde, cyanoacetylene, is produced from electric discharges under reducing conditions. The effect of pH and concentration of guanidine hydrochloride on the rate Until recently, the prebiotic synthesis of pyrimidines has of synthesis and yield of diaminopyrimidine were inves- been considered poor. The first experiments used cyano- tigated, as well as the hydrolysis of diaminopyrimidine to acetylene and cyanate to produce cytosine, but the con- cytosine, isocytosine, and uracil.Thiourea also reacts centrations of cyanate needed were rather high (0.1 M) with cyanoacetaldehyde to give 2-thiocytosine, but the (Ferris et al. 1968). An overlooked experiment in this pyrimidine yields are much lower than with guanidine paper used 1 M cyanoacetylene and 1 M urea to produce hydrochloride or urea. Thiocytosine hydrolyzes to thio- cytosine in 4.8% yield. uracil and cytosine and then to uracil. This synthesis Cyanoacetylene is produced in substantial yield from would have been a significant prebiotic source of electric discharges acting on CH 4 + N 2 mixtures 2-thiopyrimidines and 5-substituted derivatives of thio- (Sanchez et at. -
A Geochemist in His Garden of Eden
A GEOCHEMIST IN HIS GARDEN OF EDEN WALLY BROECKER 2016 ELDIGIO PRESS Table of Contents Chapter 1 Pages Introduction ................................................................................................................. 1-13 Chapter 2 Paul Gast and Larry Kulp ......................................................................................... 14-33 Chapter 3 Phil Orr...................................................................................................................... 34-49 Chapter 4 230Th Dating .............................................................................................................. 50-61 Chapter 5 Mono Lake ................................................................................................................ 62-77 Chapter 6 Bahama Banks .......................................................................................................... 78-92 Chapter 7 Doc Ewing and his Vema ........................................................................................ 93-110 Chapter 8 Heezen and Ewing ................................................................................................ 111-121 Chapter 9 GEOSECS ............................................................................................................. 122-138 Chapter 10 The Experimental Lakes Area .............................................................................. 139-151 Table of Contents Chapter 11 Sea Salt ................................................................................................................. -
Nanoconjugates Able to Cross the Blood-Brain Barrier Alexander H Stegh, Janina Paula Luciano, Samuel A
(12) STANDARD PATENT (11) Application No. AU 2017216461 B2 (19) AUSTRALIAN PATENT OFFICE (54) Title Nanoconjugates Able To Cross The Blood-Brain Barrier (51) International Patent Classification(s) A61K 31/7088 (2006.01) A61K 48/00 (2006.0 1) A61K 9/00 (2006.01) A61P 35/00 (2006.01) (21) Application No: 2017216461 (22) Date of Filing: 2017.08.15 (43) Publication Date: 2017.08.31 (43) Publication Journal Date: 2017.08.31 (44) Accepted Journal Date: 2019.10.17 (62) Divisional of: 2012308302 (71) Applicant(s) NorthwesternUniversity (72) Inventor(s) Mirkin, Chad A.;Ko, Caroline H.;Stegh, Alexander;Giljohann, David A.;Luciano, Janina;Jensen, Sam (74) Agent / Attorney WRAYS PTY LTD, L7 863 Hay St, Perth, WA, 6000, AU (56) Related Art US 2010/0233084 Al LJUBIMOVA et al. "Nanoconjugate based on polymalic acid for tumor targeting", Chemico-Biological Interactions, 2008, Vol. 171, Pages 195-203. WO 2011/028847 Al BONOU et al. "Nanotechnology approach for drug addiction therapy: Gene silencing using delivery of gold nanorod-siRNA nanoplex in dopaminergic neurons", PNAS, 2009, Vol. 106, No. 14, Pages 5546-5550. PATIL et al. "Temozolomide Delivery to Tumor Cells by a Multifunctional Nano Vehicle Based on Poly(#-L-malic acid)", Pharmaceutical Research, 2010, Vol. 27, Pages 2317-2329. ABSTRACT Polyvalent nanoconjugates address the critical challenges in therapeutic use. The single-entity, targeted therapeutic is able to cross the blood-brain barrier (BBB) and is thus effective in the treatment of central nervous system (CNS) disorders. Further, despite the tremendously high 5 cellular uptake of nanoconjugates, they exhibit no toxicity in the cell types tested thus far. -
MILLER & UREY EXPERIMENT Could Organic Molecules Assemble
CLASSWORK: ORIGINS OF CELLS PERIOD: NAME: DATE: MILLER & UREY EXPERIMENT Could organic molecules assemble under conditions on early Earth? In 1953, chemists Stanley Miller and Harold Urey tried to answer that question. They filled a sterile flask with water, to simulate the early oceans, and boiled it. To the water vapor, they added methane, ammonia, and hydrogen to simulate the gasses that they thought were in the early atmosphere. Then, as shown in the diagram, they passed the gasses through electrodes to simulate lightning. Next, they passed the gasses through a condensation chamber, where cold water cooled them, causing liquid droplets to form and return to the starting flask. The liquid circulated through the experimental set up for 1 week. The results were spectacular. From these simple molecules, they produced 21 different amino acids – the building blocks of organic proteins. 1. Explain what each part of the experiment shown below represents. (Why did Miller & Urey include each component?) Part of the Experiment: What it Represents/Why it was Included: Heated Water Mix of Gasses (Methane, Ammonia, & Hydrogen) Electric Charge 2. What conclusions can Miller & Urey draw, based on their 1953 experiment? ______________________________________________________________________________________________________________ ______________________________________________________________________________________________________________ 3. We can say that the proteins in this experiment “self-assembled.” Based on your understanding of this experiment, -
Extraction of Cyanides from Waste Solutions of Cyanidation of .Lotation
Chemistry for Sustainable Development 12 (2004) 431436 431 Extraction of Cyanides from Waste Solutions of Cyanidation of lotation Concentrates from Kholbinskoye Gold Deposit A. A. KOCHANOV1, A. A. RYAZANTSEV1, A. A. BATOEVA2, D. B. ZHALSANOVA2, A. M. BADALYAN3 and O. V. POLYAKOV3 1Siberian Transport University, Ul. D. Kovalchuk 191, Novosibirsk 630049 (Russia) E-mail: [email protected] 2Baikal Institute of Nature Management, Siberian Branch of the Russian Academy of Sciences, Ul. Sakhyanovoy 6, Ulan Ude 670047 (Russia) 3A. V. Nikolaev Institute of Inorganic Chemistry, Siberian Branch of the Russian Academy of Sciences, Pr. Akademika Lavrentyeva 3, Novosibirsk 630090 (Russia) (Received April 21, 2003; in revised form January 6, 2004) Abstract Processes that occur during extraction of cyanides from cyanidation solutions using centrifugal bubbling apparatuses (CBA) as reactors have been studied. In the eddy chamber of CBA (ðÍ < 3), one can observe virtually complete removal of HCN from solution and precipitation of heavy metals in the form of insoluble x - 1 compounds. The electronic absorption spectra of solutions treated in CBA suggest that destruction of [Cu(CN)x] , + 2+ 2 oxidation of Cu to Cu by air oxygen, and oxidation of thiocyanates in the presence of SO23, forming 2 HCN and SO4 , are also accompanied by the emergence of stable intermediate products (SCN)2 and (SCN)x of oxidation in solution. INTRODUCTION formed after acidification of the waste process- ing solutions of cyanidation to ðÍ 62.5 fol- Cyanides are widely used in extraction of lowed by absorption of HCN by alkaline solu- precious metals, mainly fine gold and silver, tions.