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lsBN978-93-5321-240-7 UNIVERSITYOF CALICUT E IYEARS lN PURSUITOF rA'ACADEMIC EXCELLENCE Re-occredited by NAAC wi,h A grode F'?.OI\T'CR5 I|\\$ me&x"&&g&&x- reMSffi&K&W Proceedingsof the GoldenJubilee National Seminar 12- 14March, zor8 pxp&xY[dEXY$F x**s_*&y Uru:VKX$'YY*P EELf;{:U" 12. A study of detection and identification of pollution 106 115 indicator bacteda from Canoly Cana1,Kozhikode Muhammedali, V. C,, SreedeviN. Kutty and Sebastian, c. D. 13. Effect of Raoiella indica (Hitst) (Acari: Tenuipalpidae) 116- 121 infestation on total nitrogen and protein conter\tsof Arecd catechuLinD, Prabheena,P. and Ramani,N. 14. Activity of carboxylesteraseenzJane in different tissuesof 122 130 Odoiporus longicollis Oliver (Coleoptera: Curculionidae) undervarious conditions oftemperature and pH Vijesh Vargheseand Gokuldas,M. 15. Species-specificassociation between the selectedFig hees 131- 136 (Ficus exasperata a]i'dFicus racemosa)and wasp species in SouthWayanad forest division Shilpa K. Satheesan, Priyadarsanan, D. R, Ashokkumar, M. and SanthoshShreevihar 16. DNA barcoding and phylogenetic analysls of Vestalis 137- 144 apicalis (Zygoptera: Calopterygidae) using cltochrome oxidaseI gene Jisha Krishnan, E. K. and Sebastian,C. D. 17. Aggegation of home invading nuisance beetle, 145 148 Mesomorphus tilliger Blal.chard 1853 (Coleoptera: Teneb onidae) and its mistakenidentity as Mupli beetle Seena,C. M. and SabuK. Thomas 18. Nah.ral enemy complex of Henosepilachna sp, 149- 152 (Coleoptera: Coccinellidae) attacking bitter gourd cultivation of Walnad, Kerala Ritty V. Jamesand SanthoshShreeYihar 19. Cenetic struchre and molecularphylogeny anallsis of 153- 159 Henosepilachna septima (Coleopten: Coccinellidae), an alarmingpest of Cucubitaceacfamily Priya Bhaskaran,li P, and Sebastian,C. D' 20. Control of Mesouorphw vil/iger" Blanchard 1853 160 164 (Coleoptera:Tenebrionidae), the confusedMupli beetleof North Kerala using its dormancy and aggregation behaviour Aswathi, P. 21. Pests and pafasitoids on organically cultivated traditional t65 - 172 rice varieties SreejinaSreedharan, K. and Nasser,M. Frontiers in Biological Resecirch 97a 93 5321-240_7 DNA BARCODING AND PITYLOGENETIC ANAI,YSIS OF VESTALIS AprcA L rS TZVCOPTERA;CALOpTERVCToae, it.s^'i. CYTOCHROMEOXJDASE I CENE Jisha.Krishnxn. , E. K,. and Sebastian,C. D. lv,urcculdrlJlotogy Laboratory, DepartrnentofZoology, universiryofcalicut, Email: [email protected] Abstract Vestalisapicalis is an emeraldcoloured damselflysperies coming under the fanily Calopterygidae.This speciesis characterisedby having durk to* green coloured head, thorax and "y.r, abdomen,brown coloreJ legs and amber cotoued wings having black tip. They are commonly observeJas groupsin forestpaths and are widely distributedthronghout Indiu, *irl., tf,"i, frlj.r, p"ut seenat Augustto September. pCR Herewe have amplifiedthe codinf seclu.nce of mitochondrialCOI geneusing suitable primer and which leldeja product having 561 bp length The sequence was depositedin the GenBank huing the accessronnunber KU510326 for future references.The phylogeneticrree constructedby Neighbourjoining methodshowed a sisterclade ..Ldonrn,p ,o '/estalisgrc iit species,another caropterygldae m€rnber.There were a totar of 553 positionsin the final dataset after eliminatingall positionscontaining gaps andmissing data. The closerelative of this speciesis foundto b fr. grniiti" by BLAST analysis.It also " con{irmedby the divergencetable plottecl by"Maximum Likely hood method.There are a total of 14 nucleotidesequences"were taken from the databasein order to make the taxonomic comparisonbetween sequences.They al providea rmique resurtthat I/. apicarisis more closeto z gracrTlsand then into y. ambalis vith respectivedivergence of0 .21 ard0.22.Aj1 the Calopterygida€members werefound to be originatedl}om one cladeshowug monophyleticancestry. We also confimed that this speciesis taxonomically more close to dragonflies (Anax pantala speratus, Jlavescence etc) than the Lepidopteran menbet (pappilio zalmoxis) taken as an outgroup. Hence the present study provided a urique DNA barcodeto this speciesand it is taxotromicallymore close to llestqlis graciliis than other Calopterygidae member. Keywords: Zygopterc, I/estalis apicalis, Molecular barcoding, Cl.tochrome oxidaseI gene Prcceedingsof the N,.tio""t S.^i"*,@ Page137 llil)/ogrt.r/ ll.rs.1rr.Jr /'>^1J-\:!178 93 532 I 214 i I r rirlr{ 'i r,1 -i : .. ,.a. , .--.. - .. -i .r'. :, ... .]*,::.:'-:;=:_r'-,',y;t:it:tt"*:ii;'t t'\ \ tt.\ltcol)l\(; .\\l) Pll\ I ociliNll lc '\NALYSIS OF l',llsTAl-ls I I't( 'ILIS (7'\ COI'l liR;\: ClAl-Ol''l'IIRYGIDAE) USrNG c Yl ocHRo\'lll oxlDAsE t G!.NI Jishl Krishnan' ll. li.,:lrd Sebastilln,C D' \lolccLrleILliologl l.lllrofalu)'.DepaltttrerrL ol Zoologl' l-lnivclsltyol Calicut' Kcralr Email: cltcdscbastianrd-i!]rllail com Abstract t (sttli\ tfiLilli.t is an emcleld colouredclalrselll) sPeciesconling undel ihe llrrill ( rrltptcr'}g-idac.lhis sllcciesis chillr-lclclisedLr)'having dark bro\ il e)'es' atnbcr sr.cn !(rloura(1heltl. thor'rr etld ilbrlonlell.bro*n colorcd lcgs and coloLr|cclrrings ltlr\'ing blacl riP- lhc) arc collrnl(rnl]obscr\ed as Eoulr\ irL pclk li)fcsLpNlhs ancl ilfe \\idcl\ disl bLllcdthrolLghoul India u'itil thcif liigLicsl sccn11 August Lo Scflcntbcf llcl-c\\'c ha\e PCR ampliliedthe codin!!seqLlcnce ol-rriLocltordfirl COI gcne using suiLxblepf;nle1 and u'hjch )'icldcd a pr'Jducl hlrling- 5(rl bp lcligLh.l hc seqtlcrrtewirs deposited in the Gcn Bank havingdre 'fi'ee acccssioDnunrl-tL KLJ5l0126 lbl lulLIe re1'elences-Ihe phylogeneti(: consh-uc1cdb)' N"'*t,,,uul.joining metl,odsho\\'ed a sislcf clade fclationshipto lc:tttlis pn:ilisspccies. anothcl- Calopte[Eidae ncmber' Thefe wcle a told] of j5l posiljrnlsin the lillal dausel aftef elilninatingall positionscontaining gaps and ruissing dalx. lhe closc relalive ()1'1hisspccies is lbund to be I/ grucilis by Lll.ASI anal)'sis.Il alsoconllrlued by the dirergcncetablc plottcd by Maximum Lihell hcxrdlrlcthod. There ar-ea lotal ol-14 nuoleotidesequences \\'erc lalien 1l-,:rnrtirc cialabase in ordq to make the taxonorric corrparisoi bet\\'een scqucnees.Thcl r1l proYi<1ca uniqueIcsuLt thitt l/ 'tpicaLisis rnore closeto i' grrrc'ilr.rand Lhen iltto l'l .rrlrl1it \\iLll rclpeclivc'livefgcnce of0 21 and 0-22A1l the Crloftef)gidxc nlcrnbcrs\\'erc lb nd 1oLt ori-qinatcdl'r'onl one cladc shn\\jrrB nronoph)lc1icxnccslr'). We. elso corllirnlcd thcl this speciesis taxonorically lrrolc closc Lo dlagonllics (Anax speratus' I'untttltt Jlatescence etc/ Lhan the l-cpidoptcran rrienrbcr' (/114rilio :a/norls) laLcn as an outgroup llence thc to tllis :jpeciesand it is Prcscnl slu.h proviclcd a urliclue DN.^ bafcodc Larorronricrlly ruore cl,lrsc l.) I/estdlis -qr./cillis than othcr Caloplerygid'rc melllltcr'. licJrvords: Z)'gopLcfa.lrcsltlis dfl(,11tt.,\IolrcLrlct brrtoding L)luchfomc oridaseIgcne "{|*.-*-''**-'-+,!E'€-*i]- 11,]*i ,:,,ro-. of rlrc N QLional Senin a. DepaflltL'?lt af Zoalo'Jlj' Uliuersitu of Calicut 137 /-Sll,\I !ll8 9i 5321 210 7 i.r1)rllr!ts'.-,. i,L liidl.]a/icrilA'cJ.L:r'-.11 _:;' :'-ii'-;:-:: -i::*.i._l:]& i'x!; ' ..-- --4.'. .-.,':,,*l-.-' i--,, -.:: -:: ' : ' I nl Iodtrctioll {)1'rhcOrder Odonala ,/-\g(r|Lcraftlrfcscllls onf ol Lhalllosl inlciclll stlbofLlef existcd250 milion commolll) cNllcdas L)arllsclllies' 11rc)ncr-e lino\\n to bc jrl the Mesozoic llra lcrfs ago alortg \ith ptintiti!e pfoLQodo alcs e\isted 'ihcl a1lbiological ((i|inraldi and Engel.2005) ar.cgeogtaphicall) distriblrted in lisLedin 309 genera fcrlms c\ccpL.{nlill licr arldconsisls ()1 2942 exLal1tspecies can be easily cNtcgorisc(lin 21ttanrilics (SLrhling" 2015)Most of the nenlbers olliny irntennae' cli.goniscllb)hd\i1r-g uiclcll sepaletedeles lusedihorax' apaii rvingsal-e sinlihr in lcgs rnd long slerldcfabdorrcn havirlg l0 scgments'lheir \'ejns fllled \\'ilh sizt-.coloLlcd or ttllcrllortLctlird supforlcd b)'nran)'cross lllcmol\nrfh (Silsbl.2(l0l ) 'bl 2-5trrI lcngLhetlsnrlll C:LloptcrlgitlNcrcpfcsclrLs 1bc oacl \ irlglctl l 5 1o illld rilcls ll i\ 'r damscllll linlill collllllonl\:obsef\'ccl irl lincst llola slrcollls jn lll2llesafc \'crr\ \\ell .lislribllLcdllimil) brnlr lelrpcflllcrn'l1fol)iclrl habitaLs an'l lcli\'cirlsLrnll\(]olldiliolls.hhasi6gcr)claNilhapprorinlNtcl)161s]l''':ics anlennac' LoDg globalll (Corcloba.1005) lhe! chali'lcLcisliclll-Yhlvc long rralcs and brorallish slcndcf abdonlen .nlc|lrilic bluc bl c coLoufc'lboci)' \\'jfi ill veinedconsjsts of lo gr-.cncololrlcd n ings in 1lmalcs Theil rlings afe hcavenly liJolrrorcanlenodallcirls.llothsexcscallbceasilydiaglosedbyhavingau'hite menbers can be \ving spol ilr lernalcu'hilc it is abscttlin Nales Mosl ofthe gcnerally lccd upon cmil)' rccogniseclto all visLlalpredalofs (Corbcl' 1999) The] Cl.rilonomidacand Culicidac (Higashi ct al . 1976)' genelally has yesl(tlis opiullis is an enlcralcl colouled Calopler'lgidae membcr colored legs gleen colotcd hcad. thorar aud ebdonlell darli bro\\'l-leyes' bro$'n 2009) Maleshave'19 andanlbcr colouled uings haring blaciilip (SrLblamaDian, witll 46-5i) mm --iimru abrlomitralleligLir,3(r-3gmm hirrcl \\ing lenglh and females (Subram'rnilu' abdoninal lengLllarl.l i8-40mm hintl \ing lcngtb fespcctivcl)' (Manoi' 2011)' 2009).-lhc1can be comuoliil obscrvedin lirrcstPalh in goups 'lhe)'afe (Emiliyamnra' dislribuledthfoughoul lndia. oflcrr seeni fofest areas to September(l\4anoj' 201 1)' l00i ) \\ ilh theirhighes( Peal( seerl at Au-sllsl accuraLe and I)NA balcocling is o Iiovel syslen 'lesigne'l to provide rapid' gcnetics arld eutonalable specics idcntiltcation
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  • Diversity and Abundance of Odonata in Palamau Tiger Reserve, Jharkhand, India

    Diversity and Abundance of Odonata in Palamau Tiger Reserve, Jharkhand, India

    Available online a t www.pelagiaresearchlibrary.com Pelagia Research Library Advances in Applied Science Research, 2014, 5(6):126-131 ISSN: 0976-8610 CODEN (USA): AASRFC Diversity and abundance of Odonata in Palamau Tiger Reserve, Jharkhand, India S. K. Sajan 1* , Jenis R. Patel 1, Manish K. Bakshi 1, Amrendra K. Singh 1, S.E.H. Kazmi 2, Anil K. Mishra 3 and P. Anand 3 1Wild India, Behind Junior DAV School, Gandhi Nagar, Kanke Road, Ranchi, Jharkhand, India 2Field Director, Palamau Tiger Reserve, Jharkhand 3Divisional Forest Officers (Buffer& Core Respectively), Palamau Tiger Reserve, Jharkhand _____________________________________________________________________________________________ ABSTRACT The Odonata survey on diversity and abundance of Palamau Tiger Reserve was carried out for the first time to gives a preliminary checklist of species within the reserve. Total 30 species of Odonata were recorded at seven different ranges of which 20 species from Anisoptera and 10 species from Zygoptera. The54% species was recorded from Libellulidae family (16) followed by 13% Coenagrionidae (4), 10% from Calopterygidae (3), 7% from both Gomphidae and Aeshnidae and 9% from others families respectively.The Shannon index shows that Kutku and Baresanar ranges have maximum diversity, whereas Chhipadohar West and Betla having least diverse area.Jaccard index indicate the similarities between Baresanar and Kutku, Betla and Chhipadohar East, Garu East and Garu West respectively.Earlier 17 species recorded from this region by Zoological Survey of India. Out of four species Rhyothemis flavescens, Trithemis pallidinervis, Potamarcha congener and Lestes viridula have been not sighted this time. Current survey deals with 13 new record for this reserve. Key words: Odonata, Diversity, Abundance, Palamau Tiger Reserve, Jharkhand _____________________________________________________________________________________________ INTRODUCTION The order Odonata comprises with dragonflies (Anisoptera) and damselflies (Zygoptera) are primitive amphibiotic insects [10][5][13].
  • Odonata (Dragonflies and Damselflies) As Bio Indicators of Water Quality

    Odonata (Dragonflies and Damselflies) As Bio Indicators of Water Quality

    ISSN(Online): 2319-8753 ISSN (Print): 2347-6710 International Journal of Innovative Research in Science, Engineering and Technology (A High Impact Factor & UGC Approved Journal) Website: www.ijirset.com Vol. 6, Issue 9, September 2017 Odonata (Dragonflies and Damselflies) as Bio Indicators of Water Quality Sonia Jacob1, A.P. Thomas2, E.K. Manju3 School of Environmental Sciences, M.G. University, Kottayam, India1&2 Department of Zoology, Alphonsa College, Pala, India3 ABSTRACT : Inorder to determine the bioindicator efficiency of odonata, their surveys were conducted and water samples were analysed from thirty ponds of Meenachil taluk at Kottayam district, Kerala. The Water Quality Index, Simpson’s Diversity Index and Species abundance values were calculated. The ponds with good water quality showed highest number of odonata species which has been contributed by the abundance of fresh water indicator species like, Bradinopyga geminata and Trithemis festiva. The odonata species, Zyxomma petiolatum and Ceriagrion cerinorubellum, which indicates the polluted water were present abundantly at bad and very bad water quality ponds. KEYWORDS: Indicator species, water quality, species abundance, odonata, pollution I. INTRODUCTION Dragonflies and damselflies are hemimetabolous insects with predominantly aquatic nymphal stage and terrestrial adult. Odonata can be found in a wide array of fresh water systems dependent on biotic and abiotic constraints. They can be seen in both lentic and lotic systems which range from tree holes to large lakes and rivers and they are hospitable for both seasonal and permanent ecosystems. Each type of water body has a characteristic species assemblage of odonata that can typically be found there. Although most species are restricted to fully aquatic environments, a few species around the world can be found as larvae in upland habitats (e.g., a few Megalagrion species are found in wet leaf litter) where relative humidity is always high (Polhemus and Asquith, 1996)[1].
  • Checklist of Odonata Species As Indicators of Riparian Ecosystem of A

    Checklist of Odonata Species As Indicators of Riparian Ecosystem of A

    Journal of Entomology and Zoology Studies 2016; 4(2): 104-108 E-ISSN: 2320-7078 P-ISSN: 2349-6800 Checklist of Odonata species as indicators of JEZS 2016; 4(2): 104-108 © 2016 JEZS riparian ecosystem of a tropical river, the southern Received: 17-01-2016 Accepted: 18-02-2016 Western Ghats, Kerala, S. India Vincy MV Department of Zoology, St. Berchmans College Vincy MV, Brilliant R, Pradeep Kumar AP Changanacherry Abstract Brilliant R A total of 36 species of odonates, including 24 species of dragonflies (Suborder Anisoptera) belonging to PG Department of 3 families and 12 species of damselflies (Suborder Zygoptera) belonging to five families were recorded Environmental Sciences, St. from the riparian zones of Meenachil River Basin, Kottayam District. The study was carried for a period John’s College, Anchal of six years from 2009-2015. The highest diversity of odonates was that of family Libellulidae (61.11%), followed by Coenagrionidae (13.89%), Calopterygidae (10.71%), Gomphidae (8.33%) and Pradeepkumar AP Department of Geology, Platycnemididae (5.56%). Six species were reported for the first time. Our data revealed odonate University of Kerala, assemblages specific to the studied habitats such as marshlands, flowing water bodies, stagnant water Kariavattom bodies and vegetation type (wet zone and dry zone). These data will be useful in future studies and conservation of biodiversity in the studied habitats. Keywords: Odonata, Meenachil River Basin, Kottayam District, biodiversity conservation 1. Introduction The damselflies (Zygoptera) and dragonflies (Anisoptera) are amphibiotic insects, which belong to the order Odonata, constitute a small, well known order of insects that are widely [1] distributed all over the world .