lsBN978-93-5321-240-7 UNIVERSITYOF CALICUT E IYEARS lN PURSUITOF rA'ACADEMIC EXCELLENCE Re-occredited by NAAC wi,h A grode

F'?.OI\T'CR5 I|\\$ me&x"&&g&&x- reMSffi&K&W Proceedingsof the GoldenJubilee National Seminar 12- 14March, zor8

pxp&xY[dEXY$F x**s_*&y Uru:VKX$'YY*P EELf;{:U" 12. A study of detection and identification of pollution 106 115 indicator bacteda from Canoly Cana1,Kozhikode Muhammedali, V. C,, SreedeviN. Kutty and Sebastian, c. D. 13. Effect of Raoiella indica (Hitst) (Acari: Tenuipalpidae) 116- 121 infestation on total nitrogen and protein conter\tsof Arecd catechuLinD, Prabheena,P. and Ramani,N. 14. Activity of carboxylesteraseenzJane in different tissuesof 122 130 Odoiporus longicollis Oliver (Coleoptera: Curculionidae) undervarious conditions oftemperature and pH Vijesh Vargheseand Gokuldas,M. 15. -specificassociation between the selectedFig hees 131- 136 (Ficus exasperata a]i'dFicus racemosa)and wasp species in SouthWayanad forest division Shilpa K. Satheesan, Priyadarsanan, D. R, Ashokkumar, M. and SanthoshShreevihar 16. DNA barcoding and phylogenetic analysls of 137- 144 apicalis (Zygoptera: ) using cltochrome oxidaseI gene Jisha Krishnan, E. K. and Sebastian,C. D. 17. Aggegation of home invading nuisance beetle, 145 148 Mesomorphus tilliger Blal.chard 1853 (Coleoptera: Teneb onidae) and its mistakenidentity as Mupli beetle Seena,C. M. and SabuK. Thomas 18. Nah.ral enemy complex of Henosepilachna sp, 149- 152 (Coleoptera: Coccinellidae) attacking bitter gourd cultivation of Walnad, Kerala Ritty V. Jamesand SanthoshShreeYihar 19. Cenetic struchre and molecularphylogeny anallsis of 153- 159 Henosepilachna septima (Coleopten: Coccinellidae), an alarmingpest of Cucubitaceacfamily Priya Bhaskaran,li P, and Sebastian,C. D' 20. Control of Mesouorphw vil/iger" Blanchard 1853 160 164 (Coleoptera:Tenebrionidae), the confusedMupli beetleof North Kerala using its dormancy and aggregation behaviour Aswathi, P. 21. Pests and pafasitoids on organically cultivated traditional t65 - 172 rice varieties SreejinaSreedharan, K. and Nasser,M. Frontiers in Biological Resecirch 97a 93 5321-240_7

DNA BARCODING AND PITYLOGENETIC ANAI,YSIS OF VESTALIS AprcA L rS TZVCOPTERA;CALOpTERVCToae, it.s^'i. CYTOCHROMEOXJDASE I CENE Jisha.Krishnxn. , E. K,. and Sebastian,C. D. lv,urcculdrlJlotogy Laboratory, DepartrnentofZoology, universiryofcalicut, Email: [email protected] Abstract

Vestalisapicalis is an emeraldcoloured damselflysperies coming under the fanily Calopterygidae.This speciesis characterisedby having durk to* green coloured head, thorax and "y.r, abdomen,brown coloreJ legs and amber cotoued wings having black tip. They are commonly observeJas groupsin forestpaths and are widely distributedthronghout Indiu, *irl., tf,"i, frlj.r, p"ut seenat Augustto September. pCR Herewe have amplifiedthe codinf seclu.nce of mitochondrialCOI geneusing suitable primer and which leldeja product having 561 bp length The sequence was depositedin the GenBank huing the accessronnunber KU510326 for future references.The phylogeneticrree constructedby Neighbourjoining methodshowed a sisterclade ..Ldonrn,p ,o '/estalisgrc iit species,another caropterygldae m€rnber.There were a totar of 553 positionsin the final dataset after eliminatingall positionscontaining gaps andmissing data. The closerelative of this speciesis foundto b fr. grniiti" by BLAST analysis.It also " con{irmedby the divergencetable plottecl by"Maximum Likely hood method.There are a total of 14 nucleotidesequences"were taken from the databasein order to make the taxonomic comparisonbetween sequences.They al providea rmique resurtthat I/. apicarisis more closeto z gracrTlsand then into y. ambalis vith respectivedivergence of0 .21 ard0.22.Aj1 the Calopterygida€members werefound to be originatedl}om one cladeshowug monophyleticancestry. We also confimed that this speciesis taxonomically more close to dragonflies (Anax pantala speratus, Jlavescence etc) than the Lepidopteran menbet (pappilio zalmoxis) taken as an outgroup. Hence the present study provided a urique DNA barcodeto this speciesand it is taxotromicallymore close to llestqlis graciliis than other Calopterygidae member.

Keywords: Zygopterc, I/estalis apicalis, Molecular barcoding, Cl.tochrome oxidaseI gene

Prcceedingsof the N,.tio""t S.^i"*,@ Page137 llil)/ogrt.r/ ll.rs.1rr.Jr /'>^1J-\:!178 93 532 I 214 i I r rirlr{ 'i r,1 -i : .. . ,.a. , .--.. - .. -i .r'. :, ... .]*,::.:'-:;=:_r'-,',y;t:it:tt"*:ii;'t

t'\ \ tt.\ltcol)l\(; .\\l) Pll\ I ociliNll lc '\NALYSIS OF l',llsTAl-ls I I't( 'ILIS (7'\ COI'l liR;\: ClAl-Ol''l'IIRYGIDAE) USrNG c Yl ocHRo\'lll oxlDAsE t G!.NI

Jishl Krishnan' ll. li.,:lrd Sebastilln,C D' \lolccLrleILliologl l.lllrofalu)'.DepaltttrerrL ol Zoologl' l-lnivclsltyol Calicut' Kcralr Email: cltcdscbastianrd-i!]rllail com Abstract

t (sttli\ tfiLilli.t is an emcleld colouredclalrselll) sPeciesconling undel ihe llrrill ( rrltptcr'}g-idac.lhis sllcciesis chillr-lclclisedLr)'having dark bro\ il e)'es' atnbcr sr.cn !(rloura(1heltl. thor'rr etld ilbrlonlell.bro*n colorcd lcgs and coloLr|cclrrings ltlr\'ing blacl riP- lhc) arc collrnl(rnl]obscr\ed as Eoulr\ irL pclk li)fcsLpNlhs ancl ilfe \\idcl\ disl bLllcdthrolLghoul India u'itil thcif liigLicsl sccn11 August Lo Scflcntbcf llcl-c\\'c ha\e PCR ampliliedthe codin!!seqLlcnce ol-rriLocltordfirl COI gcne using suiLxblepf;nle1 and u'hjch )'icldcd a pr'Jducl hlrling- 5(rl bp lcligLh.l hc seqtlcrrtewirs deposited in the Gcn Bank havingdre 'fi'ee acccssioDnunrl-tL KLJ5l0126 lbl lulLIe re1'elences-Ihe phylogeneti(: consh-uc1cdb)' N"'*t,,,uul.joining metl,odsho\\'ed a sislcf clade fclationshipto lc:tttlis pn:ilisspccies. anothcl- Calopte[Eidae ncmber' Thefe wcle a told] of j5l posiljrnlsin the lillal dausel aftef elilninatingall positionscontaining gaps and ruissing dalx. lhe closc relalive ()1'1hisspccies is lbund to be I/ grucilis by Lll.ASI anal)'sis.Il alsoconllrlued by the dirergcncetablc plottcd by Maximum Lihell hcxrdlrlcthod. There ar-ea lotal ol-14 nuoleotidesequences \\'erc lalien 1l-,:rnrtirc cialabase in ordq to make the taxonorric corrparisoi bet\\'een scqucnees.Thcl r1l proYi<1ca uniqueIcsuLt thitt l/ 'tpicaLisis rnore closeto i' grrrc'ilr.rand Lhen iltto l'l .rrlrl1it \\iLll rclpeclivc'livefgcnce of0 21 and 0-22A1l the Crloftef)gidxc nlcrnbcrs\\'erc lb nd 1oLt ori-qinatcdl'r'onl one cladc shn\\jrrB nronoph)lc1icxnccslr'). We. elso corllirnlcd thcl this speciesis taxonorically lrrolc closc Lo dlagonllics (Anax speratus' I'untttltt Jlatescence etc/ Lhan the l-cpidoptcran rrienrbcr' (/114rilio :a/norls) laLcn as an outgroup llence thc to tllis :jpeciesand it is Prcscnl slu.h proviclcd a urliclue DN.^ bafcodc Larorronricrlly ruore cl,lrsc l.) I/estdlis -qr./cillis than othcr Caloplerygid'rc melllltcr'.

licJrvords: Z)'gopLcfa.lrcsltlis dfl(,11tt.,\IolrcLrlct brrtoding L)luchfomc oridaseIgcne

"{|*.-*-''**-'-+,!E'€-*i]- 11,]*i ,:,,ro-. of rlrc N QLional Senin a. DepaflltL'?lt af Zoalo'Jlj' Uliuersitu of Calicut 137 /-Sll,\I !ll8 9i 5321 210 7 i.r1)rllr!ts'.-,. i,L liidl.]a/icrilA'cJ.L:r'-.11 _:;' :'-ii'-;:-:: -i::*.i._l:]& i'x!; ' ..-- --4.'. .-.,':,,*l-.-' i--,, -.:: -:: ' : '

I nl Iodtrctioll {)1'rhcOrder Odonala ,/-\g(r|Lcraftlrfcscllls onf ol Lhalllosl inlciclll stlbofLlef existcd250 milion commolll) cNllcdas L)arllsclllies' 11rc)ncr-e lino\\n to bc jrl the Mesozoic llra lcrfs ago alortg \ith ptintiti!e pfoLQodo alcs e\isted 'ihcl a1lbiological ((i|inraldi and Engel.2005) ar.cgeogtaphicall) distriblrted in lisLedin 309 genera fcrlms c\ccpL.{nlill licr arldconsisls ()1 2942 exLal1tspecies can be easily cNtcgorisc(lin 21ttanrilics (SLrhling" 2015)Most of the nenlbers olliny irntennae' cli.goniscllb)hd\i1r-g uiclcll sepaletedeles lusedihorax' apaii rvingsal-e sinlihr in lcgs rnd long slerldcfabdorrcn havirlg l0 scgments'lheir \'ejns fllled \\'ilh sizt-.coloLlcd or ttllcrllortLctlird supforlcd b)'nran)'cross lllcmol\nrfh (Silsbl.2(l0l ) 'bl 2-5trrI lcngLhetlsnrlll C:LloptcrlgitlNcrcpfcsclrLs 1bc oacl \ irlglctl l 5 1o illld rilcls ll i\ 'r damscllll linlill collllllonl\:obsef\'ccl irl lincst llola slrcollls jn lll2llesafc \'crr\ \\ell .lislribllLcdllimil) brnlr lelrpcflllcrn'l1fol)iclrl habitaLs an'l lcli\'cirlsLrnll\(]olldiliolls.hhasi6gcr)claNilhapprorinlNtcl)161s]l''':ics anlennac' LoDg globalll (Corcloba.1005) lhe! chali'lcLcisliclll-Yhlvc long rralcs and brorallish slcndcf abdonlen .nlc|lrilic bluc bl c coLoufc'lboci)' \\'jfi ill veinedconsjsts of lo gr-.cncololrlcd n ings in 1lmalcs Theil rlings afe hcavenly liJolrrorcanlenodallcirls.llothsexcscallbceasilydiaglosedbyhavingau'hite menbers can be \ving spol ilr lernalcu'hilc it is abscttlin Nales Mosl ofthe gcnerally lccd upon cmil)' rccogniseclto all visLlalpredalofs (Corbcl' 1999) The] Cl.rilonomidacand Culicidac (Higashi ct al . 1976)' genelally has yesl(tlis opiullis is an enlcralcl colouled Calopler'lgidae membcr colored legs gleen colotcd hcad. thorar aud ebdonlell darli bro\\'l-leyes' bro$'n 2009) Maleshave'19 andanlbcr colouled uings haring blaciilip (SrLblamaDian, witll 46-5i) mm --iimru abrlomitralleligLir,3(r-3gmm hirrcl \\ing lenglh and females (Subram'rnilu' abdoninal lengLllarl.l i8-40mm hintl \ing lcngtb fespcctivcl)' (Manoi' 2011)' 2009).-lhc1can be comuoliil obscrvedin lirrcstPalh in goups 'lhe)'afe (Emiliyamnra' dislribuledthfoughoul lndia. oflcrr seeni fofest areas to September(l\4anoj' 201 1)' l00i ) \\ ilh theirhighes( Peal( seerl at Au-sllsl accuraLe and I)NA balcocling is o Iiovel syslen 'lesigne'l to provide rapid' gcnetics arld eutonalable specics idcntiltcation b}' combinittg taxonom)" e\pcft spcries cor'r'rIr.rtct-sclcncelhat aulonlatcsthe pfoccss ol_ obtaining

Ul1ircrsitll af CalLcut P,n...-d,rLr/5 .f 1li. Natnnul SPnLlttat Depdt renl ol7'aalaqA, Page138 ir /iir)l1r.lr'(l li.s.r(rfu il /,S.rl^r978 l).3i32l 210 i,-lcntiilc.rLion.( rlrrehIorrtc r^icllsc I .lcnc is LLseclfirt bar'-co':litlgsincc il is rhc lilr!.sl ncncll loirn lhc oLherl-l lllik)cholldfiillrnltkel gencs rrld u'iclel)ttscd in ph\l{)g.n.fic slLrditsclLrc to it hls high insc|tion dcletion cVerlts llebert cl rl.-101)lSho*cd LlrxlI 6011bp fellion in the rrrtCOI genelbr aninal bar-codtn.t bcerrLscil sho\ed bigh eilicicncr lof the idcnlilicationol'bird lish flics rnd oLheIanimals. The obicctivco1-lhe prescnL sLtldy \!as to .mrplifJthe c)tochrome o\iLlNscI gclrcol' f..\1.///rapicalis b'r'PCR nlelhod inorderto plovide a uDjque | . t 'r' rrr'l' irr.;,'.' i 'lr l'-' ,) l\,lrtcrinlsind,\'lcthods

S:l!nplc CollcctioDrnd Itrcscrvrtion 'lhc ('alopfef)gidac rrlcnlbcf 1"..!I.///.!rrTrrrtrlls \l'rs collectcd li-(nl] I)llnlilir(l disL|ictol l{eral| h) harrdsu'ecp llctLirlg Nnd rerrdonl licld salrlplingDl'Lho'l \\rs uscclto covcr'thc cntirc slud\ iuca. Iclcnlilicalion*'as clonebt'obserritlg rrirr;r \cniltio1l. coloul- palieflt and gcnilaiia. clcscriltd in availablc lie)'s/idcntiflciniul guicles.Additional inlbnltaliol regafdlnBdxlc of collcction.localiLy etc'' abotll cach specimcn wiis aLsorecolded Lach speciruenwas thcn placed in a separale collccling boLtle.assigned a code nunber and slored in 7070ethanol until linlher usc. (he or norc.lcgs \{erc removcd lbr DNA isolation and kept in ethanol rrn1il ntrutcf tlsc.

DNA cxtraction,tntplificlrtion and scquencing 'Origin DNA liom selccled dragonl'lies \\rrs e)i1rccledfiorlr Ieg using DNA F\traction liit. The obloinedDNA uas conli|medusing 1oloagarose gcl About ]ng of DNA uas I'Clt amplilrcd1i) rritochorcllialcylochronle oxidasc subrmil I (COl) gcne Lrsingfbr \(afd pfimer (5' CA I Al-fGGAACCCTTTACCTG-3') and rereLscpriIDcL (5' GI-TCAIAAA(IC.\1-TG(]CAGGGI'GACG3') in Tal

DalaAnalysis

Mitochondrial CiOl sequcncedata lbr f?sldl/s apiculis was sequcncedand slrbnlillcdin GenBank(KM 510126) The eligncdsequences were usedi'or speciesidcnlificalion using NCB1 BL,A.ST.The sequencesll'on GenBAnkw€re ret,ieved and sequcnccsof cachspecies generated from this studywere compared andaiigned using CiustalW.

Results 'lhis spcciesl'las an emclald colouled Lrocl-r'\\ritllgreen colored head, thorax and abdunen. dark brown eyes. bto\\n colouled lcgs and arnber coloured wirrgs lrrling blac\lilj {l ig. I L

Figwe 1. Vestalisapicalis ' The PCR anplification ofpartial COI sequenceof tr/eslah.tapicalis isolaled fao,'-' Kerala, lndia (Fig. l) yielded a product having 561bp. The molecuiar phylogenetictree andevolutionaDr divcrgence tdble are presentedin fhe figures .respectively(Fig. 2 and1'ablc 1).

The partialamplificd producl of CO I gere of yestalisapicalis produced 56lbp scquencedDNA and it getsdeposited in 10the GcnBankwith Accessionnumber (Kl 5lUj2t') forfululereler.tr,c.

The cvolutionaryhistol) )vas inl-erledusing Neiglbour joining meLhod.The oplimal tree1\'ith the sum of branchlength :0.990.The percentageof replicate 1r-eesiowhich the associatedtaxa clusLeredtogether in the bootstraptest are sho\\4rhere. The numberofbase substitution per site from belwe€nsequences are shoun.The analyseswere rradc usingMaximum con-lposite Liiielyhood method'

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. J!9220i:l 1 Fajr]|'r ialn'lirr Figure 2: Phylogcnelic lree 01 r/esldlis icalrs jnl'erredby Neighbourioining method 'fable 1: ljstilnalc of evolutionaq'divergence bctween sequences plotled using MaxiNLrmLikcl).hood melhod

Speciesname- P€rcenlage of divergence KM5 10326Vestalls apicalis (Kerala) 0 KM675770 KM675768 Veslalis apicalis 0 KX503058 Vestalisgracilis 0.2r KF369576Vestal i s anbalis 0.22 FJ812855Ne .soba..-is ,!e1r..sl 0.20 liJ8l2847 MesoDasiisp6,si 0.21 KU565929 A nax speraltrs 0.21 KU56642i Iii the nt i s donaldson 0.21 KU565930Anar speratus 0.21 KP'I 49808 I sc hu r a tuu'o r a 0.21 KR08012'l PLtnlaI a Jlave scenc e 0.23 JQ922052 Pappil i o z.///ro).is (Lepidoptei a) 0.4

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lrcrchrrrc bcc m LiellloL ol sluLlics1o fcs(rl\c thc ph)logenl in OdonlLabilsed on r)lorpholosictllillulcs (l:r-ascr'.l9jTi lr.Lrernan.1996: llechler,. 2001). Most ()LLhc .hdfrclcfij iuc ntrinl) bascclupon \!in!r-\ienalion. rtorphologl ol 11ight JP|il|illusi1nLl copLrlalor\ stl.Lrclufcs (Pl)Lt. l99l), !\iich do not pro.luccdtobr]sl concl!5ions.Ilcrcc \\e LLSeclthe nlolcclllatnar.kef cvtochfome oxidasc I geneto l)ro\ iclf r Lrnlqucbalcorle lo llcst.tlis .qi(:alis and to inlir its phylogcny on the busi\ ol i1sI)NA scclLtcnces.llte paflial ci ing.sequelce ofthis geneviclded a ffoLlt|rl hil\jns 5(rlbp. l-hc BLr\S'l alrlllsit sho\\,cdll]at this sltcciesis 100% scqucr)ccsilnilaf 10the setlc spccicsI cltolleai liont Southcmpafl ol_Kcfala(li\4 6j5770). 'l'hc phl logcrlcticl|cc conslrlrcle.ib_r- Ncighborn. joining ntcthorlslro\\,cd thrl tltis sllccicshas (niginalcdliont a contntol)clilde (llool sl1itp\clUc . 100)\'hich gcrs splillc.l ilrlo onc clad. coDtxinsl/r.\t(li.\ opi(alis :In(l thc othct \\illt //c.!/.,,1|! !/?1.?/1.\and l/. uyticLli.:1o-r-tclitcf-indicaling V. r.rpicdli:;is laxoncnicalll,nrorc closcLo l1 gracllis (liil. 1). Thc abore resLrltalso coniirmedby lhc djvergdncc LaLrlci)lcrllod bl l\4axinrunrl-ikc11,hoocl nrethod (1able l ). Thcrc a1ea total of 14 nucleoLiclc scqLtelices\\'erc taken 1i-oltt thc database iltofdcr to mal

$..g€.},,,l,*&$ry% ta..:ee.ltitats af1h... Natot(n Se Li ar, Dep al1tt?trl of Zaataglj, Uniuercit!] oJ Caticut Pase142 l. n)t)li(!-s i1 IJiolaqicctlRescerch /S/lN t;A :t3 ii]'1-: l0 /

llc.hl\. (i- (1996).\4of pholo-sischc 0|tcfsllchungc| anl Flugclgeaderdef rc;/cnlcr libcllen und dg-cDslannn-gruppen!eftl-clcf (lltsecta: Pletleota: OLI()rrla) Lrnler besondcr-o-berucksichtiguDg dc| phylogenetischen s\slcmirlikund clcsgrrndplanes clel . Petiltria. SpecialVolunle (2): | 402.

-1. IJ)bcc- S. M.. Ogdcn.Ll:i.. l}anhanl.M. A. and Whiting.M. F. (2008). l\4olcculcs-nurphcilogl.and fbssils: A comprehensiveappr.oach to odonate fh_\loscuvilrlcl thc cv(tution of tlte odonirlewing. C1./d/.r//cr.(24):477 511.

.1. ( ofhct.P. S. (1999).I)r.agonflies: B.hn\ ioLu and ecologyof Odonata.Flssex. I {arlcl 1}ooLis.329pp. f. ('oldoba- A. A.. Adollb Coltlero l{irc|a (2005). Evolutionand ecology o1- ('xloplcr')gidae(Z)-1toflcfa: Odclnala): slatus o1-hto\\'1edgeancl l-csearch l(r..l1c\r.r.\l-t,r,t, ,1..' ll{'l: I i I li'). (r. linrilil.anrma.Ii. (1.- Raclhakishtran.C-. i\4uhanrcdJalllr l,alrl1(2005). Pict,lrial l{andbook on contrron Dragonllicsand ])antselllicsrl1- Kelala. ZoologicalSun'c). ol'lndia. l(olhata:67. 7. lrraser. F. C. ( 1957).A I'eclassilicationof the ordetOdonata. Pnbl.R. Zaol- Soc..N.S.W. S5'dn e_r..

Grirnaldj.D. andEngel. M. S. (2005).Evolutioi ofthe .Cambridge Universit) Plcss.Nerv Yorl<. Neit York. ileben. P. D. N.. Cyu'inska.A., Ball, S. L. and de Waard,J. R. (2003): Riological identilicalionsthrough DNA barcodes.Proc. R. Soc.Lond. B, (2'0): 'lj i:. l{J. Iligashi. K. (1976).Ecolos;cal stlldies on the populationof Mnaispninosa Sclvs(Odonala: Calopterldae). I. populaLion density. survival mte ancldaily acti\ilites iD thc adull damsel ll). Ph'siol.Ecologt. (.1'1): 109- 1 1 6 il Manoj.V. Naif (2011).Dragoul1ics ancl Damsellljes ol Orissaand Eastem INDIA. WildlilcOrganisatioi, Forest. 12.Plitu. ll. K. (1991), Contlibutionsol'lLr.ictional lnorphology to the plrl'logeneticsvstcmalics of Odonala.Advanc:es in Odonatologt.5: )09 l.1l.

Prc.:eedinssoftlrc NLltbnal Seininar, Depatlnent af Zootory, Uniuersit! of Calicut pa8c143 l t1)ttti.trs it1 liirlo.clical l?t::;r:arch ISIIN:978 93.532t 210 7 ll. IlchD. A. (': (2001). I'hllogencticl|rl)'sis ol'highc. lcrel r'elationshipsol Od(nrirtir.Sr:t/erzl1r.' lintomololly- 28: 18l' 2)9.

1.1.Silsby. J. (2001). D|agonfiiesoi'thc woll.l. Smithsonianhstitution Press, \\ !rJlrirl!lorr D( .

15. SLrbrarnar'rian.l( A. (2009): Dragontlieso[lndia - A tield guide Vigyan Prasar.Ncw Delhi.pp. l-i68.

16.SL:1.iling.F.. Sahlen.G., Corb. S., Kalliman-V. J Djjkstra,K. D. B. and van -l-d. 'Ihorp. l. (2015)."Orclel Odonata". ln: .lancs-andChristopher Rogels. t). {Ed.) Iicologl xnd gcner.albiolog1. Tholp and Covicb's llreshwalel ln\ertcbrales(4 ed.).Acadcmic Prcss. pf. 893 932. 'l'anrLn 1'/. a. l(.. Slccl'ref-G.- Pelersoi.I).- lrlipsl(y-D. andKumar', S (201i): MECA 6 Moleculafii\,oluliolra) (ienelic Analysis Velsion 6. Molecular 17li-l"li) 13',,1.rg1and t rulurion..i0: t8. l.l.Lreman.J. W. 1I. (1996).A prelilnirlarycladistics analysis o1'odonate wing vu\aliot1.Odanalologica.25 (1): 59 12.

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