DOCSLIB.ORG

The Mechanism Whereby Bile Acid Micelles Increase the Rate of Fatty Acid and Cholesterol Uptake Into the Intestinal Mucosal Cell

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
The Mechanism Whereby Bile Acid Micelles Increase the Rate of Fatty Acid and Cholesterol Uptake Into the Intestinal Mucosal Cell The mechanism whereby bile acid micelles increase the rate of fatty acid and cholesterol uptake into the intestinal mucosal cell. H Westergaard, J M Dietschy J Clin Invest. 1976;58(1):97-108. https://doi.org/10.1172/JCI108465. Research Article Studies were undertaken to define the mechanism whereby bile acid facilitates fatty acid and cholesterol uptake into the intestinal mucosal cell. Initial studies showed that the rate of uptake (Jd) of several fatty acids and cholesterol was a linear function of the concentration of these molecules in the bulk phase if the concentration of bile acid was kept constant. In contrast, Jd decreased markedly when the concentration of bile acid was increased relative to that of the probe molecule but remained essentially constant when the concentration of both the bile acid and probe molecule was increased in parallel. In other studies Jd for lauric acid measured from solutions containing either 0 or 20 mM taurodeoxycholate and saturated with the fatty acid equaled 79.8+/-5.2 and 120.8+/-9.4 nmol.min(-1).100 mg(-1), respectively: after correction for unstirred layer resistance, however, the former value equaled 113.5+/-7.1 nmol.min(- 1).100 mg(-1). Maximum values of Jd for the saturated fatty acids with 12, 16, and 18 carbons equaled 120.8+/-9.4, 24.1+/-3.2, and 13.6+/-1.1 nmol.min(-1).100 mg(-1), respectively. These values essentially equaled those derived by multiplying the maximum solubility times the passive permeability coefficients appropriate for each of these compounds. The theoretical equations were then derived that define the expected behavior of Jd for the various lipids under these different experimental circumstances where the mechanism of absorption was assumed to occur either by […] Find the latest version: https://jci.me/108465/pdf The Mechanism Whereby Bile Acid Micelles Increase the Rate of Fatty Acid and Cholesterol Uptake into the Intestinal Mucosal Cell HEmN WESTEGAABD and JoHN M. DImrsCmY From the Gastrointestinal-Liver Section of the Department of Medicine, The University of Texas Health Science Center at Dallas, Dallas, Texas 75235 A B s T R A C T Studies were undertaken to define the phase of lipid molecules in equilibrium with the micelle. mechanism whereby bile acid facilitates fatty acid and The experimental results were consistent both qualita- cholesterol uptake into the intestinal mucosal cell. Initial tively and quantitatively with the third model indicating studies showed that the rate of uptake (Jd) of several that the principle role of the micelle in facilitating lipid fatty acids and cholesterol was a linear function of the absorption is to overcome unstirred layer resistance concentration of these molecules in the bulk phase if the while the actual process of fatty acid and cholesterol concentration of bile acid was kept constant. In contrast, absorption occurs through a monomer phase in equi- Jd decreased markedly when the concentration of bile librium with the micelle. acid was increased relative to that of the probe molecule but remained essentially constant when the concentra- INTRODUCTION tion of both the bile acid and probe molecule was in- In various studies carried out in animals as well as in creased in parallel. In other studies Jd for lauric acid man it has been demonstrated that bile acids an measured from solutions containing either 0 or 20 mM play in the of taurodeoxycholate and saturated with the fatty acid important rol-e facilitating absorption various equaled 79.8±5.2 and 120.8±9.4 100 dietary fats by the gastrointestinal tract. There are nmol[min-' mg1, in the respectively: after correction for unstirred layer resist- quantitative differences, however, dependency of various lipids on the presence of these ance, however, the former value equaled nmol micelle-forming 113.5±7.1 of medium chain min-' 100 mg-'. Maximum values of Jd for the saturated compounds. Fatty acids length, for example, are absorbed as well in the absence as fatty acids with 12, 16, and 18 carbons equaled 120.8±9.4, nearly in the presence of bile acid micelles. As the chain length 24.1±3.2, and 13.6±+1.1 nmol min-' 100 mg-', respec- is increased the become more tively. These values essentially equaled those derived fatty acids progressively the of the bile acid micelle by multiplying the maximum solubility times the passive dependent upon presence for efficient uptake into the mucosal in the case permeability coefficients appropriate for each of these cell, and, of very such as essen- compounds. The theoretical equations were then derived nonpolar compounds cholesterol, no occurs in the absence of bile acids that define the expected behavior of Jd for the various tially absorption considerable work in area there is lipids under these different experimental circumstances (1-5). Despite this little information with the exact mecha- where the mechanism of absorption was assumed to oc- currently dealing nism(s) whereby the bile acid micelle enhances cur either by uptake of the whole micelle, during inter- actually the rate of into the intestinal mucosal cell. action of the micelle with an infinite number of sites lipid uptake However, the recent observation that an unstirred water on the microvillus membrane or through a monomer layer adjacent to the luminal cell surface, and not the Dr. Westergaard was a postdoctoral research fellow in microvillus membrane, is actually rate limiting to the gastroenterology while these studies were undertaken. His uptake of long chain fatty acids and steroids (6, 7) current address is Medical Department P, University Hos- raises the possibility that the major physiological func- pital, DK-2100 Copenhagen 0, Denmark. Received for publication 19 August 1975 and in revised tion of the bile acid micelle is to overcome this resist- form 2 March 1976. ance and so augment lipid absorption. The Journal of Clinical Investigation Volume 58 July 1976 97-108 97 It is equally unclear how the individual lipid molecules to be used for determination of tissue uptake rates. The carried in the micelle are actually transferred into the distribution of C" on thin-layer chromatography of the entire fatty acids was the same before and after these preparations. mucosal cell. Earlier workers postulated that the Measurement of tissue uptake. Tissue uptake rates of micellar particle might be taken up into the intestinal fatty acids, cholesterol, and taurodeoxycholate were deter- cell intact (8, 9). However, more recent work from mined with a technique which has been described in detail several laboratories has shown that the various constitu- (15). Briefly, unfasted albino New Zealand rabbits weigh- ent molecules carried in the micelle are absorbed at ap- ing 2-2.5 kg were killed by decapitation, and a 15-20-cm portion of the proximal jejunum was removed. The intestine parently independent rates suggesting that uptake must was rinsed, cut in circular pieces, and mounted in chambers occur by some other mechanism (10-12). The two most that expose 0.78 cm' of the mucosal surface to the incuba- likely alternative possibilities are that molecular transfer tion solutions. The mounted tissue preparations were kept at occurs via an intermediate, obligatory monomer phase 4°C in oxygenated Krebs-bicarbonate buffer until used in the cell mem- various studies. At the start of an experiment the chambers or that transfer takes place directly into the were transferred to preincubation beakers with oxygenated brane during "collision" or interaction between the Krebs-bicarbonate buffer at 37°C to equilibrate the tissue micelle and the lipid-protein phase of the microvillus for 8 min at this temperature. The chambers were then membrane. transferred to the incubation beakers containing the ["C] the is two- probe molecule and ['H] dextran and incubated for the The purpose of present study, therefore, desired length of time. The preincubation and incubation fold. First, experiments are described that define in both buffers were all maintained at pH 7.40, and solutions were qualitative and quantitative terms the manner in which stirred at identical stirring rates with circular magnetic bars. the bile acid micelle facilitates lipid absorption. Second, In all studies the stirring rate was kept constant at 600 rpm other studies are presented that allow differentiation as using a strobe light (7, 15). At the end of the incubation the chambers were removed from the solutions, and the to which mechanism is operative during the actual tissue was briefly rinsed in cold 0.9% NaCl solution. The transfer of lipid molecules from the bile acid micelle into exposed tissue was then cut out, divided into two pieces, the intestinal mucosal cell. and placed in tared scintillation vials. The tissue was dried overnight to determine dry weight and was then saponified METHODS with NaOH. Scintillation fluid was added and radioactivity was determined by means of an external standardization Chemicals. Unlabeled and [1-"C] long chain fatty acids, technique to correct for variable quenching of the two iso- unlabeled and [4-"C] cholesterol and unlabeled f-sitosterol topes (16). The rate of uptake, (Jd),' was calculated after were all > 99% pure as supplied by Applied Science Labs, the total ["C] radioactivity had been corrected for the Inc., State College, Pa. Unlabeled taurodeoxycholic acid amount of the probe molecule present in the adherent mu- was from Sigma Chemical Co., St. Louis, Mo. and [24-"C] cosal fluid volume. These rates are expressed as the nano- sodium taurodeoxycholate was from Mallinckrodt Inc., St. moles of the probe molecule taken up into the mucosal cells Louis, Mo. The purity of these compounds was > 98% as per minute per 100 mg of dry tissue weight (nmol.min-' 100 checked by thin layer chromatography (13, 14). Glucose mg-') was obtained from J.
Load more

Recommended publications
  • Micelle Formation and the Hydrophobic Effect†
    6778 J. Phys. Chem. B 2004, 108, 6778-6781 Micelle Formation and the Hydrophobic Effect† Lutz Maibaum,‡ Aaron R. Dinner,§ and David Chandler*,‡ Department of Chemistry, UniVersity of California, Berkeley, California 94720, and Department of Chemistry, UniVersity of Chicago, Chicago, Illinois 60637 ReceiVed: NoVember 14, 2003; In Final Form: January 14, 2004 The tendency of amphiphilic molecules to form micelles in aqueous solution is a consequence of the hydrophobic effect. The fundamental difference between micelle assembly and macroscopic phase separation is the stoichiometric constraint that frustrates the demixing of polar and hydrophobic groups. We present a theory for micelle assembly that combines the account of this constraint with a description of the hydrophobic driving force. The latter arises from the length scale dependence of aqueous solvation. The theoretical predictions for temperature dependence and surfactant chain length dependence of critical micelle concentrations for nonionic surfactants agree favorably with experiment. I. Introduction in accord with experimental observations. An appendix is used to augment the discussion in section II. This paper concerns the formation of micelles, which are the simplest form of amphiphilic assemblies. Our treatment of this II. Theory phenomenon is based upon the length scale dependence of hydrophobic effects.1,2 Namely, the free energy to solvate small A. Law of Mass Action. We consider an aqueous solution hydrophobic molecules scales linearly with solute volume, of neutral amphiphilic molecules (i.e., nonionic surfactants), each whereas that to solvate large hydrophobic species scales linearly of which has a single alkyl chain as its hydrophobic tail. In with surface area. The crossover from one regime to the other general, amphiphiles can form aggregates of various sizes and occurs when the oily species presents a surface in water shapes.
    [Show full text]
  • Tutorial on Working with Micelles and Other Model Membranes
    Tutorial on Working with Micelles and Model Membranes Chuck Sanders Dept. of Biochemistry, Dept. of Medicine, and Center for Structural Biology Vanderbilt University School of Medicine. http://structbio.vanderbilt.edu/sanders/ March, 2017 There are two general classes of membrane proteins. This presentation is on working with integral MPs, which traditionally could be removed from the membrane only by dissolving the membrane with detergents or organic solvents. Multilamellar Vesicles: onion-like assemblies. Each layer is one bilayer. A thin layer of water separates each bilayer. MLVs are what form when lipid powders are dispersed in water. They form spontaneously. Cryo-EM Micrograph of a Multilamellar Vesicle (K. Mittendorf, C. Sanders, and M. Ohi) Unilamellar Multilamellar Vesicle Vesicle Advances in Anesthesia 32(1):133-147 · 2014 Energy from sonication, physical manipulation (such as extrusion by forcing MLV dispersions through filters with fixed pore sizes), or some other high energy mechanism is required to convert multilayered bilayer assemblies into unilamellar vesicles. If the MLVs contain a membrane protein then you should worry about whether the protein will survive these procedures in folded and functional form. Vesicles can also be prepared by dissolving lipids using detergents and then removing the detergent using BioBeads-SM dialysis, size exclusion chromatography or by diluting the solution to below the detergent’s critical micelle concentration. These are much gentler methods that a membrane protein may well survive with intact structure and function. From: Avanti Polar Lipids Catalog Bilayers can undergo phase transitions at a critical temperature, Tm. Native bilayers are usually in the fluid (liquid crystalline) phase.
    [Show full text]
  • Rheology of Surfactants: Wormlike Micelles and Lamellar Liquid Crystalline Phase - O
    RHEOLOGY - Vol. II - Rheology of Surfactants: Wormlike Micelles and Lamellar Liquid Crystalline Phase - O. Manero, F. Bautista, J. E. Puig RHEOLOGY OF SURFACTANTS: WORMLIKE MICELLES AND LAMELLAR LIQUID CRYSTALLINE PHASES O. Manero Instituto de Investigaciones en Materiales, Universidad Nacional Autónoma de México, A.P. 70-360, México, D.F., 04510 F. Bautista Departamento de Física, Universidad de Guadalajara, Guadalajara, Jal., 44430, México J. E. Puig Departamento de Ingeniería Química, Universidad de Guadalajara, Guadalajara, Jal., 44430, México Keywords: Surfactants. Rheology, Linear viscoelasticity, Nonlinear viscoelasticity, Wormlike micelles, Lamellar liquid crystalline phases, Shear banding, Shear thickening, Stability, Heterogeneous flows, Flow-concentration coupling. Contents 1. Introduction 2. Wormlike Micellar Systems 2.1. Linear Viscoelasticity 2.2. Non-linear Viscoelasticiy 2.2.1. Shear banding Flow 2.2.2. Transient flows 2.2.3. Stability 2.3. Shear banding and linear viscoelasticity 2.4. Flow concentration coupling 2.5. Shear thickening 3. Surfactant Lamellar Liquid Crystal Phases 4. Concluding Remarks Acknowledgements Glossary UNESCO – EOLSS Bibliography Biographical Sketches Summary SAMPLE CHAPTERS In this chapter, the rheology of a special class of complex fluids is analyzed and reviewed. In particular, attention is focused on the dynamic behavior of systems constituted by aqueous phases of surfactants, namely, micellar solutions and lamellar liquid crystalline phases. Surfactants are molecules that possess a polar head and a non- polar tail (i.e., amphiphiles). The polar head may be nonionic or it may posses a charge, in which case they are classified as anionic (negatively charged), cationic (positively charged) and zwitterionic or amphoteric. In ionic surfactants, the polar head is balanced ©Encyclopedia of Life Support Systems (EOLSS) RHEOLOGY - Vol.
    [Show full text]
  • Micelle and Bilayer Formation of Amphiphilic Janus Particles in a Slit-Pore
    Int. J. Mol. Sci. 2012, 13, 9431-9446; doi:10.3390/ijms13089431 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 www.mdpi.com/journal/ijms Article Micelle and Bilayer Formation of Amphiphilic Janus Particles in a Slit-Pore Gerald Rosenthal * and Sabine H. L. Klapp Institute of Theoretical Physics, School II, Technical University Berlin, Sec. EW 7-1, Hardenbergstr. 36, Berlin D-10623, Germany; E-Mail: [email protected] * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +49-30-314-23763; Fax: +49-30-314-21130. Received: 5 June 2012; in revised form: 17 July 2012 / Accepted: 18 July 2012 / Published: 26 July 2012 Abstract: We employ molecular dynamics simulations to investigate the self-assembly of amphiphilic Janus particles in a slit-pore consisting of two plane-parallel, soft walls. The Janus particles are modeled as soft spheres with an embedded unit vector pointing from the hydrophobic to the hydrophilic hemisphere. The structure formation is analyzed via cluster size distributions, density and polarization profiles, and in-plane correlation functions. At low temperatures and densities, the dominating structures are spherical micelles, whereas at higher densities we also observe wall-induced bilayer formation. Finally, we compare the MD results with those from a previous density functional study. Keywords: Janus particles; amphiphilic systems; confinement; cluster formation; micelles; icosahedrons; bilayers 1. Introduction The term “Janus”-particles (named after the two-faced roman god) generally refers to particles composed of at least two chemically or physically distinctive surfaces. Significant experimental progress over the last years (see, e.g., [1–6]) nowadays allows us to create a wide class of Janus particles, including particles with “conventional” amphiphilic, but also with electric dipolar or quadrupolar, and even magnetic properties.
    [Show full text]
  • Membrane Protein Structure Determination and Characterisation by Solution and Solid-State NMR
    biology Review Membrane Protein Structure Determination and Characterisation by Solution and Solid-State NMR Vivien Yeh , Alice Goode and Boyan B. Bonev * School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK; [email protected] (V.Y.); [email protected] (A.G.) * Correspondence: [email protected] Received: 21 October 2020; Accepted: 11 November 2020; Published: 12 November 2020 Simple Summary: Cells, life’s smallest units, are defined within the enclosure of thin, continuous membranes, which confine the molecular machinery required for the life and replication of cells. Crucially, membranes of cells establish and actively maintain distinctly different environments inside cells, including electrical and solute gradients vital to normal cellular functions. Membrane proteins are in charge of transport, electrical polarisation, signalling, membrane remodelling and other important functions. As such, membrane proteins are key drug targets and understanding their structure and function is essential to drug development and cellular control. Membrane proteins have physical characteristics that make such studies very challenging. Nuclear magnetic resonance is one advanced tool that enables structural studies of membrane proteins and their interactions at the atomic level of detail. We discuss the applications of NMR in solution and solid state to membrane protein studies alongside new developments in signal and sensitivity enhancement through dynamic nuclear polarisation. Abstract: Biological membranes define the interface of life and its basic unit, the cell. Membrane proteins play key roles in membrane functions, yet their structure and mechanisms remain poorly understood. Breakthroughs in crystallography and electron microscopy have invigorated structural analysis while failing to characterise key functional interactions with lipids, small molecules and membrane modulators, as well as their conformational polymorphism and dynamics.
    [Show full text]
  • Micelles, Dispersions, and Liquid Crystals in the Catanionic Mixture Bile Saltdouble-Chained Surfactant. the Bile Salt-Rich Area
    Langmuir 2000, 16, 8255-8262 8255 Micelles, Dispersions, and Liquid Crystals in the Catanionic Mixture Bile Salt-Double-Chained Surfactant. The Bile Salt-Rich Area Eduardo F. Marques,*,†,‡ Oren Regev,§ Håkan Edlund,| and Ali Khan† Physical Chemistry 1, Center for Chemistry and Chemical Engineering, P.O. Box 124, Lund University, Lund SE-221 00, Sweden, Department of Chemical Engineering, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, Departamento de Quı´mica, Universidade de Coimbra, 3049 Coimbra, Portugal, and Department of Chemistry and Process Technology, Chemistry, Mid Sweden University, SE-851 70 Sundsvall, Sweden Received February 15, 2000. In Final Form: August 1, 2000 The phase behavior and phase structure for the catanionic pair sodium taurodeoxycholate-didodecyl- dimethylammonium bromide (DDAB) are investigated, at 25 °C. A combination of techniques is used including light and electron microscopy, small-angle X-ray scattering, and pulsed field gradient NMR self-diffusion. The bile salt micellar solution incorporates large amounts of the double-chained amphiphile, with the solution region extending to equimolarity. On the contrary, the hexagonal liquid-crystalline phase is destabilized by the addition of small amounts of DDAB. At equimolarity, coacervation instead of precipitation is observed, with formation of a viscous isotropic solution and a very dilute one. In the water-rich part of the phase diagram, a peculiar type of phase separation occurs, involving the formation of very fine bluish dispersions and a region of coexistence of two dispersions (double dispersion region). Microscopy and self-diffusion data for the solution region indicate limited growth of the mixed micelles.
    [Show full text]
  • Effects of Hydrophobic Chain Length on the Micelles of Heptaoxyethylene Hexadecyl C16E7 and Octadecyl C18E7 Ethers
    Polymer Journal, Vol. 37, No. 5, pp. 368–375 (2005) Effects of Hydrophobic Chain Length on the Micelles of Heptaoxyethylene Hexadecyl C16E7 and Octadecyl C18E7 Ethers y Yoshiyuki EINAGA, Ai KUSUMOTO, and Akane NODA Department of Chemistry, Nara Women’s University, Kitauoya Nishi-machi, Nara 630-8506, Japan (Received January 4, 2005; Accepted February 1, 2005; Published May 15, 2005) ABSTRACT: Micelles of heptaoxyethylene hexadecyl C16E7 and octadecyl C18E7 ethers in dilute aqueous solu- tions were characterized at finite surfactant concentrations c by static (SLS) and dynamic light scattering (DLS) experi- ments at several temperatures T below the critical points, in order to examine the effects of hydrophobic chain length on size, shape, structure, etc., of the micelles. The SLS results were successfully analyzed by the thermodynamic theory formulated with wormlike spherocylinder model for SLS of micelle solutions, thereby yielding the molar mass Mw of the micelles as a function of c and the cross-sectional diameter d, indicating that the micelles assume a flexible spher- 2 1=2 ocylindrical shape. The radius of gyration hS i and hydrodynamic radius RH of the micelles as functions of Mw were found to be also well described by the corresponding theories for the wormlike spherocylinder or wormlike chain mod- els. The micelles grow in size with increasing T to greater length for longer hydrophobic chain, i.e., alkyl group of the surfactants. They were rather flexible compared to the micelles formed with other polyoxyethylen alkyl ethers. The cross-sectional diameter d and spacings s between adjacent hexaoxyethylene chains on the micellar surface were sim- ilar in the C16E7 and C18E7 micelles.
    [Show full text]
  • Rapid Critical Micelle Concentration (CMC) Determination Using Fluorescence Polarization Analysis of the Physical-Chemical Properties of Detergents
    Application Note Critical Micelle Concentration Determination Using Fluorescence Polarization Rapid Critical Micelle Concentration (CMC) Determination Using Fluorescence Polarization Analysis of the Physical-Chemical Properties of Detergents Paul Held Ph. D., Lab Manager, Applications Department, BioTek Instruments, Inc., Winooski, VT The determination of the critical micelle concentration (CMC) value of surfactants under different environmental conditions is important for a number of different biological and chemical processes. Because the CMC is not a constant value, shifting with different environmental conditions, it is important that a rapid, reliable and easy methodology be available to facilitate testing. Here we describe the rapid semi-automated determination of CMC values for surfactants in 384-well microplates using fluorescence polarization. Introduction Surfactants are amphiphilic compounds that have a molecular structure containing both a hydrophilic (water loving) and a hydrophobic (water hating) region. The hydrophobic region is usually a long chain aliphatic hydrocarbon, whereas the hydrophilic portion can be composed of an ionic or non-ionic polar group. The physical nature of these molecules bestows the ability to reduce surface tension of solutions and to self aggregate into colloids known as micelles. A micelle is an aggregation of surfactant Figure 1. Schematic drawing of Micelle formation. Key Words: molecules in a colloidal suspension. A typical micelle in aqueous solution forms with the hydrophilic head regions in contact with the Amphiphilic surfactants have numerous properties Fluorescence Polarization water and the hydrophobic aliphatic tail regions other than lowering of surface tension and are Critical Micelle Concentration buried in the inner portion of the micelle. Useful often labeled as to the use (e.g.
    [Show full text]
  • 1 (199-210)病毒14-4期3475换.Indd
    VIROLOGICA SINICA 2014, 29 (4): 199-210 DOI 10.1007/s12250-014-3475-1 RESEARCH ARTICLE Labeling of infl uenza viruses with synthetic fl uorescent and biotin-labeled lipids Natalia A Ilyushina1#, Evgeny S Chernyy2#, Elena Y Korchagina2, Aleksandra S Gambaryan3, Stephen M Henry4, Nicolai V Bovin2 1. Food and Drug Administration, 29 Lincoln Drive, Bethesda 20892, MD, USA 2. Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho Maklaya 16/10, Moscow 117997, Russia 3. Chumakov Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences, Moscow Region 142782, Russia 4. Biotechnology Research Institute, AUT University, Private Bag 92006, Auckland 1142, New Zealand Direct labeling of virus particles is a powerful tool for the visualization of virus–cell interaction events. However, this technique involves the chemical modification of viral proteins that affects viral biological properties. Here we describe an alternative approach of influenza virus labeling that utilizes Function-Spacer-Lipid (FSL) constructs that can be gently inserted into the virus membrane. We assessed whether labeling with fl uorescent (fl uo-Ad-DOPE) or biotin-labeled (biot- CMG2-DOPE) probes has any deleterious effect on influenza virus hemagglutinin (HA) receptor specificity, neuraminidase (NA) activity, or replicative ability in vitro. Our data clearly show that neither construct signifi cantly affected infl uenza virus infectivity or viral affi nity to sialyl receptors. Neither construct infl uenced the NA activities of the infl uenza viruses tested, except the A/Puerto Rico/8/34 (H1N1) strain. Our data indicate that lipid labeling provides a powerful tool to analyze infl uenza virus infection in vitro.
    [Show full text]
  • Molecular Thermodynamics of Micellization: Micelle Size Distributions and Geometry Transitions
    Brazilian Journal of Chemical ISSN 0104-6632 Printed in Brazil Engineering www.abeq.org.br/bjche Vol. 33, No. 03, pp. 515 - 523, July - September, 2016 dx.doi.org/10.1590/0104-6632.20160333s20150129 MOLECULAR THERMODYNAMICS OF MICELLIZATION: MICELLE SIZE DISTRIBUTIONS AND GEOMETRY TRANSITIONS M. S. Santos1, F. W. Tavares1,2* and E. C. Biscaia Jr1 1Programa de Engenharia Química/COPPE, Universidade Federal do Rio de Janeiro, C.P. 68502, CEP: 21941-972, Rio de Janeiro, RJ - Brazil. E-mail: [email protected] 2Escola de Química, Universidade Federal do Rio de Janeiro, CEP: 21941-909, Rio de Janeiro - RJ, Brazil. (Submitted: February 26, 2015 ; Revised: June 10, 2015 ; Accepted: June 18, 2015) Abstract - Surfactants are amphiphilic molecules that can spontaneously self-assemble in solution, forming structures known as micelles. Variations in temperature, pH, and electrolyte concentration imply changes in the interactions between surfactants and micelle stability conditions, including micelle size distribution and micelle shape. Here, molecular thermodynamics is used to describe and predict conditions of micelle formation in surfactant solutions by directly calculating the minimum Gibbs free energy of the system, corresponding to the most stable condition of the surfactant solution. In order to find it, the proposed methodology takes into account the micelle size distribution and two possible geometries (spherical and spherocylindrical). We propose a numerical optimization methodology where the minimum free energy can be reached faster and in a more reliable way. The proposed models predict the critical micelle concentration well when compared to experimental data, and also predict the effect of salt on micelle geometry transitions.
    [Show full text]
  • Preparation of Nanoliposomes by Microfluidic Mixing in Herring-Bone
    www.nature.com/scientificreports OPEN Preparation of nanoliposomes by microfuidic mixing in herring-bone channel and the role of membrane fuidity in liposomes formation Jan Kotouček1,8, František Hubatka1,8, Josef Mašek1, Pavel Kulich1, Kamila Velínská1, Jaroslava Bezděková1,2, Martina Fojtíková1, Eliška Bartheldyová1, Andrea Tomečková1, Jana Stráská3, Dominik Hrebík4, Stuart Macaulay5, Irena Kratochvílová 6*, Milan Raška 1,7* & Jaroslav Turánek1* Introduction of microfuidic mixing technique opens a new door for preparation of the liposomes and lipid-based nanoparticles by on-chip technologies that are applicable in a laboratory and industrial scale. This study demonstrates the role of phospholipid bilayer fragment as the key intermediate in the mechanism of liposome formation by microfuidic mixing in the channel with “herring-bone” geometry used with the instrument NanoAssemblr. The fuidity of the lipid bilayer expressed as fuorescence anisotropy of the probe N,N,N-Trimethyl-4-(6-phenyl-1,3,5-hexatrien-1-yl) was found to be the basic parameter afecting the fnal size of formed liposomes prepared by microfuidic mixing of an ethanol solution of lipids and water phase. Both saturated and unsaturated lipids together with various content of cholesterol were used for liposome preparation and it was demonstrated, that an increase in fuidity results in a decrease of liposome size as analyzed by DLS. Gadolinium chelating lipids were used to visualize the fne structure of liposomes and bilayer fragments by CryoTEM. Experimental data and theoretical calculations are in good accordance with the theory of lipid disc micelle vesiculation. Liposomes, self-assembled nanoparticles based on lipid bilayers are widely employed for biomedical and bio- technological purposes1.
    [Show full text]
  • Conventional and Nanotechnology Based Approaches to Combat Chronic Obstructive Pulmonary Disease: Implications for Chronic Airway Diseases
    International Journal of Nanomedicine Dovepress open access to scientific and medical research Open Access Full Text Article REVIEW Conventional and Nanotechnology Based Approaches to Combat Chronic Obstructive Pulmonary Disease: Implications for Chronic Airway Diseases This article was published in the following Dove Press journal: International Journal of Nanomedicine Mehak Passi1,* Abstract: Chronic obstructive pulmonary disease (COPD) is the most prevalent obstructive Sadia Shahid 2,* lung disease worldwide characterized by decline in lung function. It is associated with airway Sankarakuttalam Chockalingam2 obstruction, oxidative stress, chronic inflammation, mucus hypersecretion, and enhanced Isaac Kirubakaran Sundar 3 autophagy and cellular senescence. Cigarette smoke being the major risk factor, other Gopinath Packirisamy 1,2 secondary risk factors such as the exposure to air pollutants, occupational exposure to gases and fumes in developing countries, also contribute to the pathogenesis of COPD. 1 Nanobiotechnology Laboratory, Centre Conventional therapeutic strategies of COPD are based on anti-oxidant and anti-inflamma- for Nanotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, tory drugs. However, traditional anti-oxidant pharmacological therapies are commonly used Uttarakhand, India; 2Department of to alleviate the impact of COPD as they have many associated repercussions such as low Biotechnology, Indian Institute of diffusion rate and inappropriate drug pharmacokinetics. Recent advances in nanotechnology Technology
    [Show full text]