CURRICULUM VITAE Mohammad A Qasim Department of Chemistry Indiana University Purdue University Fort Wayne Fort Wayne, IN 46805 Tel: (765) 714-6597(C); (260)481-6678 (O) E-mail:[email protected]

EDUCATION: 1978: Ph. D. (Biochemistry), Aligarh M. University, India 1975: M. Phil. (Biochemistry), Aligarh M. University, India 1973: M. Sc. (Biochemistry), Aligarh M. University, India 1971: B. Sc. (Chemistry & Biology), Aligarh M. University, India

TEACHING EXPERIENCE: 2010 - 2012: Taught following courses during the years 2010 to 2012: (i) Introduction to Biochemistry (CHM53400) (ii) Biochemistry Lab (CHM53500) (iii) Introduction to Biochemistry (CHM53300) (iv) General Chemistry Lecture and Lab (CHM11200) (v) General Chemistry Lab (CHM11100) (vi) General Chemistry Lecture (CHM11100) (vii) Seminars in Chemistry (CHM49500) (viii) Advances in Chemistry I (CHM49600) (ix) Advances in Chemistry II (CHM49700)

Fall 2009 Taught following courses at IPFW during Fall 2009 semester: (i) Introductory Biochemistry (CHM533) (ii) General Chemistry Lecture and Lab (CHM112) (iii) General Chemistry Lab (CHM111)

2006-2009: Taught following courses to undergraduate students at Xavier University of Louisiana, New Orleans: (i) Introduction to Biochemistry (CHEM4130) (ii) Introduction to Biochemistry Lab (CHEM4130L) (iii) General Chemistry Lab I (CHEM1011L) (iv) General Chemistry Lab II (CHEM1021L)

1981-1994: Taught Biochemistry lecture and laboratory courses to Medical students at J. N. Medical College, Aligarh M. University, Aligarh (INDIA). Topics covered in the lecture course included chemistry and metabolism of carbohydrates, lipids, proteins and nucleic acids, membrane structure and transport across membranes. The lab. course involved qualitative tests on carbohydrates and lipids and quantitative determinations of biologically important compounds such as glucose, urea, uric acid, creatinin, cholesterol, bilirubin etc.

EXPERIENCE AS RESEARCH MENTOR:

2009-2012: Guided eight undergraduate research students in the Chemistry Department at IPFW

2006-2009: Guided three undergraduate research students at Xavier University of Louisiana.

1994- 2005 While working in various research capacities at Purdue University, I guided several undergraduate students in their research. These students were registered with Professor Michael Laskowski Jr. at Purdue who gave me the responsibility for their day to day guidance.

1981-1994: Successfully guided six Ph. D. students to their graduate degrees. Several research papers were published during this time in peer reviewed journals. They are listed in “list of publications” below. Many of my graduate students are serving as faculty in academic institutions in different countries such as Malasia, Saudi Arabia, and United States.

RESEARCH FUNDING: 2009-2011: Submitted following research proposals in the year 2009, 2010, and 2011. All of them were accepted for funding. (i) IPFW Faculty summer research grant, December 2009. (ii) Senior Research grant of Indiana Academy of Science, October 2010. (iii) IPFW Faculty summer research grant, December 2010.

2006-2009: Submitted two research grant applications to Center for Undergraduate Research (CUR) at Xavier. Both of these applications were accepted for funding – one in March 2007 and the other in October 2008.

1981-1994: As independent researcher in India I applied for three major research grants to central granting agencies such as Indian Council of Medical Research (ICMR), Council of Scientific and Industrial Research (CSIR), and Department of Science and Technology (DST). All of these grant applications were approved for funding.

JOB HISTORY: 2009- Assistant Professor, Department of Chemistry, Indiana University Purdue University at Fort Wayne, IN 46805

2006-2009: Associate Professor of Chemistry, Department of Chemistry, Xavier University of Louisiana, New Orleans, LA 70125

1999-2005: Research Scientist/Associate Research Scientist Purdue University, Department of Chemistry West Lafayette, Indiana, USA

1999 –1999: Assistant Research Scientist, Purdue University, Department of Chemistry, West Lafayette, Indiana, USA

1997 -1999 Research Associate, Purdue University, Department of Chemistry, West Lafayette, Indiana, USA

1994 -1997: Postdoctoral Research Fellow,Purdue University, Department of Chemistry, West Lafayette, Indiana USA

1990 -1994: Reader, Aligarh M. University, Department of Biochemistry, J. N. Medical College, Aligarh, India

1992 -1993: DAAD Fellow, Westfalische Wilhelms U., Institut fur Physikalische Chemie, Munster, Germany,

1987 -1990: Postdoctoral Research Fellow, Purdue University, Department of Chemistry, West Lafayette, Indiana USA.

1981 -1987 Lecturer, Aligarh M. University, Department of Biochemistry, J. N. Medical College, Aligarh, India

1979 -1980 DANIDA Fellow, Danish Institute of Protein Chemistry Horsholm, Denmark

AFFILIATIONS: I have been or am a member of various prestigious scientific societies: Member of Society of Biological Chemists, India, 1975. Member, American Association for the Advancement of Science, 1979. Member, Protein Society, 1988. Nominated member, Purdue University Chapter of Sigma Xi, 1995. Nominated member, American Society for Biochemistry and Molecular Biology, 1996. Member of Indiana Academy of Science. 2010

RESEARCH PUBLICATIONS/ARTICLES: 1. Qasim. M. A. and Taha, M. (2013) “Investigation of the influence of guanidine hydrochloride on inhibitor-protease association ” Protein and peptide Letters 20, 187-191.

2. Qasim, M. A. (2012) “Streptogrisin A” in Handbook of Proteolytic , 3rd edition, Elsevier Academic Press (Eds. Barrett, A. J., Rawlings, N. D., Woessner, J. F.), pp 2546-2548.

3. Qasim, M. A. (2012) “Streptogrisin B” in Handbook of Proteolytic Enzymes, 3rd edition, Elsevier Academic Press (Eds. Barrett, A. J., Rawlings, N. D., Woessner, J. F.) pp. 2549-2555.

4. Cleavage of peptide bonds bearing ionizable amino acids at P1 by serine proteases with hydrophobic S1 pocket. Qasim, M. A., Song, J., Markley, J. L. and Laskowski, M. Jr. (2010) Biochem. Biophys. Res. Commun. 400, 507-510.

5. Structural insights into the non-additivity effects in the sequence to reactivity algorithm for serine peptidases and their inhibitors. Lee, T-W., Qasim, M. A., Laskowski, M. Jr., and James, M. N. G. (2007) J. Mol. Biol. 367, 527-546.

6. Despite having a common P1 Leu, eglin c inhibits α-lytic proteinase a million- fold more strongly than does turkey ovomucoid third domain. Qasim, M. A., Van Etten, R. L., Yeh, T., Saunders, C., Ganz, P. J., Qasim, S., Wang, L., and Laskowski, M. Jr. (2006) Biochemistry 45, 11342-11248.

7. Functional evolution within a . Yi, Z., Vitek, O., Qasim, M. A., Lu, S. M., Lu, W., Ranjbar, M., Laskowski, M. C., Bailey-Kellogg, C. and Laskowski, M. Jr. (2006) Proteins, 63, 697-708.

8. Ring-Toss: Capping highly exposed tyrosyl or tryptophyl residues in proteins with beta-cyclodextrin. Yi, Z., Qasim, M. A., Qasim, S., Warrington, T. L. and Laskowski, M. Jr. (2005) Biochim. Biophys. Acta 1760, 372-377.

9. The role of scaffolding in standard mechanism serine proteinase inhibitors. Kelly, C. A., Laskowski, M. Jr. and Qasim, M. A. (2005) Prot. Peptide Lett. 12, 465- 471.

10. From the discovery of the reactive site of inhibitors to their sequence to reactivity algorithm. Qasim, M. A. (2005) Prot. Peptide Lett. 12, 397-401.

11. Structure of the Carlsberg-OMTKY3 complex reveals two different ovomucoid conformations. Maynes, J. T., Cherney, M. M., Qasim, M. A., Laskowski, M. Jr. and James, M. N. (2005) Acta Crystallogr. D Biol. Crystallogr. 61, 580-588.

12. Testing of the additivity-based protein sequence to reactivity algorithm. Qasim, M. A., Lu, W., Lu, S., Ranjbar, M., Yi, Z-Y., Chiang, Y-W., Ryan, K., Anderson, S., Zhang, W-L., Qasim, S. and Laskowski, M. Jr. (2003) Biochemistry 42, 6460-6466.

13. Two conformational states of turkey ovomucoid third domain at low pH three- dimensional structures, internal dynamics, and interconversion kinetics and thermodynamics. Song, J., Laskowski, M. Jr., Qasim, M. A. and Markley, J. L. (2003) Biochemistry 42, 6380-6391.

14. NMR Determination of pKa values for Asp, Glu, His, and Lys mutants at each variable contiguous -inhibitor contact position of the turkey ovomucoid third domain. Song, J., Laskowski, M. Jr., Qasim, M. A. and Markley, J. L. (2003) Biochemistry 42, 2847-2856

15. Additivity based prediction of equilibrium constants for some protein-protein associations. Laskowski, M. Jr., Qasim, M. A., and Yi, Z-P. (2003) Cur. Opin. Str. Biol. 13, 130-139.

16. Contribution of peptide bonds to inhibitor-protease binding: crystal structures of the turkey ovomucoid third domain backbone variants OMTKY3-Pro18I and OMTKY3-ψ[COO]-Leu18I in complex with Streptomyces griseus proteinase B + (SGPB) and the structure of the free inhibitor, OMTKY3-ψ[CH2NH2 ]-Asp19I. Bateman, K. S., Huang, K., Anderson, S., Lu, W., Qasim, M. A., Laskowski, M. Jr. and James, M. N. G. (2001) J. Mol. Biol. 305, 839-849.

17. Predicting the reactivity of proteins from their sequence alone: Kazal family of protein inhibitors of serine proteinases. Lu, S. M., Lu, W., Qasim, M. A., Anderson, S., Apostol, I., Ardelt, W., Bigler, T., Chiang, Y-W., Cook, J., James, M. N. G., Kato, I., Kelly, C., Kohr, W., Komiyama, T., Lin, T-Y., Ogawa, M., Otlewski, J., Park, S. J., Qasim, S., Ranjbar, M., Tashiro, M., Warne, N., Whatley, H., Wieczorek, A., Wieczorek, M., Wilusz, T., Wynn, R., Zhang, W. and Laskowski, M. Jr. (2001) Proc. Nat. Acad. Sci. 98, 1410-1415.

18. Deleterious effects of β-branched residues in the S1 specificity pocket of Streptomyces griseus proteinase B, SGPB: Crystal structures of the turkey ovomucoid third domain variants Ile18I, Val18I, Thr18I, and Ser18I in complex with SGPB. Bateman, K. S., Anderson, S., Lu, W., Qasim, M. A., Laskowski, M. Jr. and James, M. N. G. (2000) Prot. Sci. 9, 83-94.

19. Interaction in standard mechanism, canonical protein inhibitors with serine proteinases. Laskowski, M. Jr. and Qasim, M. A. and Lu, S. M. (2000) in Protein-protein Recognition (C. Kleanthous, Ed.) Oxford University Press, 228- 279.

20. What can the structures of enzyme-inhibitor complexes tell us about the structures of enzyme-substrate complexes. Laskowski, M. Jr. and Qasim, M. A. (2000) Biochim. Biophys. Acta 7, 324-337.

21. Deleterious effects of -branched residues in the S1 specificity pocket of Streptomyces griseus proteinase B (SGPB): crystal structures of the turkey ovomucoid third domain variants Ile18I, Val18I, Thr18I, and Ser18I in complex with SGPB. Bateman, K. S., Anderson, S., Lu, W., Qasim, M. A., Laskowski, M. Jr. and James, M. N. G. (2000) Protein Science 9, 83-94.

22. Use of fluorescence enhancement technique to study bilirubin-albumin interaction. Athar, H., Ahmad, N., Tayyab, S., and Qasim, M. A. (1999) Int. J. Biol. Macromol. 25, 353-358.

23. Use of fluorescence enhancement technique to study bilirubin-albumin interaction. Athar, H., Ahmad, N., Tayyab, S., and Qasim, M. A. (1999) Int. J. Biol. Macromol. 25, 353-358.

24. Expression and characterization of inhibitors from the ascarid nematodes Anisakis simplex and Ascaris suum. Nguyen, T.T., Qasim, M.A., Morris, S., Lu, C.-C., Hill, D., Laskowski, Jr., M., and Sakanari, J.A. (1999) Molecular and Biochemical Parasitology 102, 79-89.

25. Molecular basis of indomethacin-human serum albumin interaction. Trivedi, V. D., Vorum, H., Honoroe, B. and Qasim, M. A. (1999) J. Pharm. Pharmacol. 51, 591-600.

26. Interscaffolding additivity. Association of P1 variants of eglin c and of turkey ovomucoid third domain with serine proteinases. Qasim, M. A., Ganz, P. J., Saunders, C., Bateman, K.S., James, M. N. G. and Laskowski, M. Jr. (1997) Biochemistry 36, 1598-1607.

27. Probing intermolecular main chain H-bonding in serine proteinase-protein inhibitor complexes: Chemical synthesis of backbone engineered turkey ovomucoid third domain. Lu, W., Qasim, M. A., Laskowski, M. Jr., and Kent, S. B. H. (1997) Biochemistry 36, 673-679.

28. Binding of amino acid side chains to S1 cavities of serine proteinases. Lu, W., Apostol, I., Qasim, M. A., Warne, N., Wynn, R., Zhang, W., Anderson, S., Chiang, Y-W., Ogin, E., Rothberg, I., Ryan, K. and Laskowski, M. Jr. (1997) J. Mol. Biol. 266, 441-461.

29. Interaction of bromocresol green with different serum albumins studied by fluorescence quenching. Trivedi, V. D., Saxena, I., Siddiqui, M. U., and Qasim, M. A. (1997) Biochem. Mol. Biol. Int. 43, 1-8.

30. Total chemical synthesis of ovomucoid third domain by both stepwise elongation and native chemical ligation solid phase peptide synthesis Lu, W., Qasim, M. A. and Kent, S. B. H. (1996) J. Am. Chem. Soc. 118, 8518-8523.

31. Isolation and characterization of domain I of ovoinhibitor. Galzie, Z., Qasim, M. A. and Salahuddin, A. (1996) Biochim. Biophys. Acta 1293, 113-121.

32. Ionizable P1 residues in serine proteinase inhibitors undergo large pK shifts on complex formation. Qasim, M. A., Ranjbar, M., Wynn, R., Anderson, S. and Laskowski, M. Jr. (1995) J. Biol. Chem. 270, 27403-27406.

33. Fatty acid binding to bovine serum albumin prevents formation of intermediate in its denaturation. Ahmad, N. and Qasim, M. A. (1995) Eur. J. Biochem. 227, 563- 565. 34. A study of acid phosphatase system in semen, pre and post coital vaginal fluids. Hanif, S.A. Rizvi, S, J., Qasim, M. A., Arshad, Z. And Husain, M. (1994) J. Forensic Med. Toxicol. 11, 1-4.

35. Changes in protein stability upon chemical modification of lysine residues of bovine serum albumin by different reagents. Fazili, K. M., Mir, M. M. and Qasim, M. A. (1993) Biochem. Mol. Biol. Int. 31, 807-816.

36. Conformation and bilirubin binding properties of acetylated, carbamylated and guanidinated bovine serum albumins. Fazili, K. M., Mir, M. M. and Qasim, M. A. (1993) Biochem. (Life Sci. Advances) 12, 81-88.

37. Chemical modification of buried lysine residues of bovine serum albumin and its influence on protein conformation and bilirubin binding. Mir, M. M., Fazili, K. M. and Qasim, M. A. (1992) Biochim. Biophys. Acta 1119, 261-267.

38. Amino acid sequences of ovomucoid third domains from 25 additional species of birds. Laskowski, M. Jr., Apostol, I., Ardelt, W., Cook, J., Giletto, A., Kelly, C. A., Lu, W., Park, S. J., Qasim, M. A., Whatley, H. E., Wieczorek, A. and Wynn, R. (1990) J. Prot. Chem. 9, 715-725.

39. Binding of bromophenol blue to bovine serum albumin and its succinylated forms. Tayyab, S. and Qasim, M. A. (1990) Int. J. Biol. Macromol. 12, 55-58.

40. On the binding of congo red to bovine serum albumin and its succinylated forms. Tayyab, S. and Qasim, M. A. (1990) Med. Sci. Res. 18, 413-414.

41. Purification and properties of buffalo serum albumin. Tayyab, S. and Qasim, M. A. (1990) Biochem. Int. 20, 405-415.

42. Noninvolvement of surface lysine residues of bovine serum albumin in bilirubin binding. Tayyab, S. and Qasim, M. A. (1989) Biochem. Int. 18, 343-349.

43. Serum albumin: A universal biocarrier. Tayyab, S., Mir, M. M. and Qasim, M. A. (1989) Med. Sci. Res. 17, 1-4.

44. Bilirubin-albumin binding: An approach towards prevention of kernecterus in infants. Tayyab, S. and Qasim, M. A. (1989) Med. Sci. Res. 16, 893-895.

45. Biochemistry and roles of glycophorin. Tayyab, S. and Qasim, M. A. (1988) Biochem. Edu. 16, 63-66.

46. Major sialoglycoprotein (glycophorin A) of buffalo erythrocyte membrane. Tayyab, S. and Qasim, M. A. (1988) Med. Sci. Res. 16, 1213-1214.

47. Influence of surface charge on the solubility behavior of porcine gamma globulin. Mir, M. M., Fazili, K. M. and Qasim, M. A. (1988) Med. Sci. Res. 16, 463-464.

48. Influence of succinylation of bovine serum albumin on its conformation and bilirubin binding. Tayyab, S. and Qasim, M. A. (1987) Biochim. Biophys. Acta 913, 359-367. 49. Altered N---F transition in bovine serum albumin dimer and tetramer. Mir, M. M. and Qasim, M. A. (1986) Biochem. Int. 13, 487-491.

50. A correlation between changes in conformation and molecular properties of bovine serum albumin upon succinylation. Tayyab, S. and Qasim, M. A. (1986) J. Biochem. 100, 1125-1136.

51. Purification and some properties of buffalo liver B. Fazili, K. M. and Qasim, M. A. (1986) J. Biochem. 100, 292-299.

52. A possible correlation between the salting out behavior of proteins and their surface hydrophobicity. Salahuddin, A., Waseem, A., Khan, M. Y. and Sibghatullah (1983) Ind. J. Biochem. Biophys. 20, 127-131.

53. Influence of salts of Hofmeister series on the salting out behavior of bovine and buffalo serum albumins. Qasim, M. A. and Salahuddin, A. (1981) Ind. J. Biochem. Biophys. 18, 393-397.

54. Buffalo immunoglobulin G--effect of lyotropic salts on its solubility. Salahuddin, A., Irshad, M. and Qasim, M. A. (1981) Ind. J. Biochem. Biophys. 18, 317-321.

55. Solubility of serum albumins in ammonium sulfate solutions with special reference to its dependence on protein conformation. Qasim, M. A., Salahuddin, A. and Sibghatullah (1981) Ind. J. Biochem. Biophys. 18, 15-21.

56. Some observations on serum haptoglobin changes in cases of viral hepatitis and obstructive jaundice. Bilgrami, G., Tayagi, S. P. and Qasim, M. A. (1981) Ind. J. Pathol. Microbiol. 24, 285-289.

57. N-(9-xanthenyl) amino acid N-carboxyanhydrides in the solid phase synthesis of lys-lys linked cyclic leucine enkephalin. Halstrom, J., Qasim, M. A., Brunfeldt, K. and Nebelin, E. (1981) Hoppe Seylers Z. Physiol. Chem. 362, 593-599.

58. Fluorescent, liquid crystalline polyamino acids: potential photoconductors and polymeric supports. Synthesis and polymerization of w-9-carbazolyl carbonyl - lysine, -ornithine and -tyrosine anhydrides. Halstrom, J., Kovacs, K., Brunfeldt, K. and Qasim, M. A. (1980) Proceedings of the 16th European Peptide Symposium held at Helsingor (Denmark) from Aug. 31 to Sep. 6, 1980, pp 759- 767.

59. Serum haptoglobins in cases of ischaemic heart disease. Bilgrami, G., Tayagi, S. P. and Qasim, M. A. (1980) Bilgrami, G., Tayagi, S. P. And Qasim, M. A. (1980) Japanese Heart Journal 21, 505-510.

60. The conformational consequences of maleylation of amino groups of ovalbumin. Qasim, M. and Salahuddin, A. (1979) J. Biochem. 85, 1027-1035.

61. Changes in conformation and immunological activity of ovalbumin during its modification with different acid anhydrides. Qasim, M. A. and Salahuddin, A. (1978) Biochim. Biophys. Acta 536, 50-63.

62. Characterization of stable conformational states in urea induced conformational transitions of ovomucoid. Waheed, A., Qasim, M. A. and Salahuddin, A. (1977) Eur. J. Biochem. 76, 383-390.

63. Ionization of tyrosyl groups of ovalbumin under native and denaturing conditions. Qasim, M. A. and Salahuddin, A. (1977) Biochim. Biophys. Acta 490, 515-522.

RESEARCH PAPERS PRESENTED IN MAJOR CONFERENCES: ~50

Research Papers Presented since 2009: 1. Mohammad A. Qasim and MohammadTaha “Mechanism of Protein Denaturation Guandine Hydrochloride induced Dissociation of Inhibitor-Protease Complexes” Paper presented at the 126th Annual Meeting of Indiana Academy of Science held in Indianapolis on March 5, 2011, p9.

2. Josiah McMillin and Swatabdi Kamal (Mentor: Mohammad Qasim) “Stability of Red Kidney Bean Inhibitor to Heat and Pepsin Digestion” Poster presented at IUURC in Indianapolis on November 16, 2012.

3. Cortnee Hyser (Mentor: Mohammad Qasim) “Heat and Pepsin Stable Serine Protease Inhibitor from Pinto Bean” Poster presented at IUURC in Indianapolis on November 16, 2012.