DOCSLIB.ORG

Lina Zybartaitė Keturbriaunės Jonaţolės (Hypericum Maculatum Crantz)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Lina Zybartaitė Keturbriaunės Jonaţolės (Hypericum Maculatum Crantz) VYTAUTO DIDŢIOJO UNIVERSITETAS GAMTOS MOKSLŲ FAKULTETAS BIOLOGIJOS KATEDRA LINA ZYBARTAITĖ KETURBRIAUNĖS JONAŢOLĖS (HYPERICUM MACULATUM CRANTZ) LIETUVOS POPULIACIJŲ GENETINĖ ĮVAIROVĖ Magistro baigiamasis darbas Biologijos studijų programa, valstybinis kodas 62401B107 Biologijos studijų kryptis Vadovas: prof. habil. dr. Eugenija Kupčinskienė ______ ______ (Mokls. laipsnis, vardas, pavardė) (Parašas) (Data) Apginta: prof. h.p. Algimantas Paulauskas ______ _____ (Fakulteto/studijų instituto dekanas/direktorius) (Parašas) (Data) Kaunas, 2011 Darbas atliktas: 2010–2011 m. Vytauto Didţiojo universitete, Biologijos katedroje, Paduvos universitete, Aplinkosauginės agronomijos ir augalininkystės mokslų katedroje Recenzentas: dr. Brigita Tamutė Darbas ginamas: viešame magistrų darbų gynimo komisijos posėdyje 2011 metų birţelio 2 d. Vytauto Didţiojo universitete, Biologijos katedroje, 801 auditorijoje. Adresas: Vileikos g. 8, LT-44404 Kaunas, Lietuva. Protokolo Nr. darbo vykdytojo pavardė ir parašas: ___________________________________ mokslinio vadovo(-ai) pavardė ir parašas: _______________________________ katedros, atsakingos uţ magistro darbo parengimą, Biologijos katedra pavadinimas, jos vedėjo pavardė ir parašas A. Sruoga __________________ 2 TURINYS SANTRUMPOS................................................................................................................................. 5 SANTRAUKA................................................................................................................................... 7 SUMMARY....................................................................................................................................... 8 ĮVADAS............................................................................................................................................ 9 1. LITERATŪROS APŢVALGA...................................................................................................... 11 1.1. Jonaţolės (Hypericum spp.) genties sistematinė, morfologinė-fiziologinė charakteristika........................................................................................................................ 11 1.2. Jonaţolių citogenetiniai poţymiai.................................................................................. 13 1.3. Jonaţolių antriniai metabolitai........................................................................................ 13 1.4. Jonaţolių genetiniai tyrimo metodai............................................................................... 16 1.4.1. Atsitiktinai pagausintos polimorfinės DNR metodo pritaikymas.................... 17 1.4.2. Numatytų sekų polimerazinės grandininės reakcijos metodas........................ 20 1.4.3. Amplifikuotų ir restrikcinių fragmentų ilgio polimorfizmo metodo pritaikymas................................................................................................................. 21 1.4.4. Mikrosatelitinių ţymenų metodo pritaikymas................................................. 23 1.4.5. Kiti metodai taikomi genetinės įvairovės tyrimuose....................................... 25 1.5. Jonaţolių genetinio profilio ryšys su antriniais junginiais............................................. 27 2. MEDŢIAGA IR METODIKA....................................................................................................... 29 2.1. Augalinės medţiagos rinkimo vietos.............................................................................. 29 2.2. DNR išskyrimas.............................................................................................................. 31 2.3. APPD analizės sąlygos................................................................................................... 32 2.4. Populiacijų APPD analizės sąlygos................................................................................ 33 2.5. AP-PGR analizės sąlygos............................................................................................... 34 2.6. AP-PGR produktų valymas............................................................................................ 35 2.7. PKS analizės sąlygos...................................................................................................... 36 2.8. DNR elektroforezė agarozės gelyje................................................................................ 38 2.9. PGR fragmento ekstrakcija iš agarozės gelio................................................................. 39 2.10. PGR fragmento pakartotinas pagausinimas.................................................................. 41 2.11. PGR fragmento klonavimo sąlygos.............................................................................. 41 2.12. Plazmidţių išskyrimas iš ląstelių.................................................................................. 43 2.13. Kapiliarinė elektroforezė ir DNR sekoskaita................................................................ 44 3 2.14. Statistinė duomenų analizė........................................................................................... 44 3. TYRIMŲ REZULTATAI IR JŲ APTARIMAS........................................................................... 46 3.1. DNR išskyrimo iš keturbriaunės jonaţolės metodo parinkimas..................................... 46 3.2. APPD metodo sąlygų optimizavimas keturbriaunės jonaţolės genetinės įvairovės tyrimams................................................................................................................................ 46 3.2.1. APPD PGR mišinio sudėties parinkimas........................................................... 46 3.2.2. APPD DNR pagausinimo programos sąlygų optimizavimas............................. 46 3.3. AP-PGR metodo sąlygų optimizavimas keturbriaunės jonaţolės genetinės įvairovės tyrimams................................................................................................................................ 48 3.4. PKS metodo sąlygų optimizavimas keturbriaunės jonaţolės genetinės įvairovės tyrimams................................................................................................................................ 56 3.4.1. Mikrosatelitinių pradmenų kūrimas.................................................................... 56 3.4.2. PKS metodo PGR mišinio sudėties parinkimas................................................. 57 3.4.3. PKS metodo pagausinimo programos sąlygų optimizavimas............................ 58 3.4.4. PGR fragmento klonavimas................................................................................ 59 3.5. Keturbriaunės jonaţolės populiacijų genetinės įvairovė, įvertinta APPD metodu....... 61 IŠVADOS.......................................................................................................................................... 72 LITERATŪROS SĄRAŠAS............................................................................................................. 74 PADĖKA........................................................................................................................................... 80 MOKSLINĖS PUBLIKACIJOS IR PRANEŠIMAI......................................................................... 81 PRIEDAI............................................................................................................................................ 83 4 SANTRUMPOS AFIP – amplifikuotų fragmentų ilgio polimorfizmas (angl. Amplified Fragment Length Polymorphism) AMOVA – molekulinės genetinės įvairovės analizė (angl. Analysis of Molecular Variance) AOX – alternatyvios oksidazės genas (angl. Alternative oxidase gene) APPD – atsitiktinai pagausinta polimorfinė DNR (angl. Random Amplified Polymorphic DNA, RAPD) AP-PGR – numatytų sekų polimerazinė grandininė reakcija (angl. Arbitrarily Primed Polymerase Chain Reaction – AP-PCR) bp – bazių pora (angl. base pair) CTAB – cetiltrimetilamonio bromidas (angl. Cetyltrimethylammonium bromide) DNR – deoksiribonukleorūgštis (angl. Deoxyribonucleic acid, DNA) dNTP – deoksiribonukleotidai FCSS – srovės citometrinis sėklų įvertinimas (angl. Flow Cytometric Seed Screen, FCSS) GDxy – genetinis atstumas tarp x ir y genotipų (angl. Genetic Distance) Gst – genetinės diferenciacijos koeficientas (Nei, 1973) H – genetinė įvairovė (Nei, 1973) I – Šenono (Shannon‟o) informacinis indeksas ITS – transkriptų vidiniai skirtukai (angl. Internal Transcribed Spacer, ITS) IVNP – introno vieno nukleotido polimorfizmas (angl. Intron Single Nucleotide Polymorphism, ISNP) LC-MS/MS – skystinė chromatografija, masių spektrometrija Na – stebimas alelių skaičius lokuse NCBI – nacionalinis biotechnologinės informacijos centras (angl. National Center for Biotechnology Information) Ne – efektyvių alelių skaičius lokuse Nm – genų srautas tarp populiacijų (angl. Gene flow) P – polimorfinių lokusų procentas PGR – polimerazinė grandininė reakcija (angl. Polymerase Chain Reaction, PCR) PKA – principinių koordinačių analizė (angl. Principal Coordinates Analysis, PCA) PKS – paprastosios kartotinės sekos (angl. Simple Sequence Repeat, SSR) PPSI – paprastųjų pasikartojančių sekų intarpai (angl. Inter-Simples Sequence Repeats, ISSR) RFIP – restrikcijos fragmentų ilgio polimorfizmas (angl. Restriction Fragment Length Polymorphism, RFLP) SDS – natrio dodecilsulfatas (angl. Sodium dodecyl sulphate) 5 UPGMA – porų grupavimo pagal aritmetinius vidurkius metodas su vienodais svoriais (angl. Unweighted Pair-Group Method of arithmetic Averages) ΦPT – fiksacijos indekso FST analogas, populiacijų diferenciacijos
Load more

Recommended publications
  • Hawkes CV (2004) Effects of Biological Soil Crusts on Seed Germination of Four Endangered Herbs in a Xeric Florida Shrubland During Drought
    Hawkes CV (2004) Effects of biological soil crusts on seed germination of four endangered herbs in a xeric Florida shrubland during drought. Plant Ecol 170:121- 134 In a south-central Florida rosemary shrubland, the effects of biological soil crusts on the germination of four small-seeded herbs (Eryngium cuneifolium, Hypericum cumulicola, Paronychia chartacea, and Polygonella basiramia) were studied using a series of greenhouse and field experiments. This study sought to determine propitious environmental conditions for the continued survival of these four federally endangered species and further develop our limited understanding of the effects of biological soil crusts on the germination of vascular plants. In the greenhouse experiment, three of the four species (Eryngium cuneifolium, Hypericum cumulicola, and Paronychia chartacea) showed significantly greater germination in pots with crust left intact than in pots with destroyed, or autoclaved crust. The other species (Polygonella basiramia) showed no significant difference in germination between the two treatments. In the field experiment, plots were established in which resident soil crusts were either left intact, flamed, or mechanically disturbed. To investigate the effects of time since fire and distance to dominant shrub (Florida rosemary, Ceratiola ericoides), plots were set-up in three ages of postfire and two distances from the dominant shrub (away and near). Seeds of all four species were disseminated in field plots and checked monthly for germination. One species, Hypericum cumulicola, showed significant levels of increased germination in plots with crust as opposed to burned or disturbed plots. Germination was low for all four herbs and each species showed a unique response to effects of soil crust disturbance, time since fire, and distance to C.
    [Show full text]
  • Appendix D to ERP Application Listed Species Occurrences and Project
    Appendix D to ERP Application NUCOR Steel Micro Mill Appendix D to ERP Application Listed Species Occurrences and Project Effects for Nucor Steel Micro Mill in Avon Park, Polk County, Florida This listed species occurrence information and project effects discussion is supplemental to the Wetlands and Habitat Assessment Technical Memorandum (Appendix C), which is also attached to the ERP application. See Appendix C for project description and location map. This supplemental information is provided to assist the technical review of the project’s potential effect on Federal and State listed species and/or their habitats. Included with this supplemental information are these figures: Figure D1 – Wildlife Observations Onsite and within 1 Mile Radius Figure D2 – Wildlife Observation Records and Crossings within 5 Mile Radius Figure D3 – Site Photographs May-June 2018 Wildlife and Habitat Onsite Habitat The entire area is currently managed citrus cultivation. No natural upland habitats remain in the project area. A small area of mature oak trees near the former home site remains near Pabor Lake to the south. Construction contractors’ trailers will be parked in that area. Access roads of compacted imported clay fill soil or compacted sand and densely grass, encircle and traverse the groves. These conditions can be seen in site photographs attached to this Appendix D. The citrus groves offer minimal habitat requirements for very few listed species. No wetland habitats or other surface water habitats occur within the project footprint, including the proposed rail spur, and access roads. Offsite Habitat The micro mill will be over 1000 feet from any remaining non-cultivated uplands east and west of the proposed development site.
    [Show full text]
  • This Article Was Originally Published in a Journal Published by Elsevier
    This article was originally published in a journal published by Elsevier, and the attached copy is provided by Elsevier for the author’s benefit and for the benefit of the author’s institution, for non-commercial research and educational use including without limitation use in instruction at your institution, sending it to specific colleagues that you know, and providing a copy to your institution’s administrator. All other uses, reproduction and distribution, including without limitation commercial reprints, selling or licensing copies or access, or posting on open internet sites, your personal or institution’s website or repository, are prohibited. For exceptions, permission may be sought for such use through Elsevier’s permissions site at: http://www.elsevier.com/locate/permissionusematerial BIOLOGICAL CONSERVATION 137 (2007) 263– 270 available at www.sciencedirect.com journal homepage: www.elsevier.com/locate/biocon Comparative demography of a rare species in Florida scrub and road habitats Pedro F. Quintana-Ascencioa,b,*, Carl W. Weekleyb, Eric S. Mengesb aUniversity of Central Florida, Department of Biology, 4000 Central Florida Boulevard, Orlando, FL 32816-2368, United States bArchbold Biological Station, P.O. Box 2057, Lake Placid, FL 33862, United States ARTICLE INFO ABSTRACT Article history: Anthropogenic habitats can offer opportunities for expansion of rare species. The federally Received 2 October 2006 listed herb Hypericum cumulicola is virtually restricted to natural gaps within fire-main- Received in revised form tained Florida scrub, but also occurs within and along sandy roads traversing scrub. To test 29 January 2007 the hypothesis that sandy roads provide suitable habitat for H. cumulicola, we compared the Accepted 14 February 2007 demographic performance of scrub and road populations at the Lake Wales Ridge State For- Available online 2 April 2007 est and Archbold Biological Station in south-central Florida.
    [Show full text]
  • Copyright Statement
    University of Plymouth PEARL https://pearl.plymouth.ac.uk 04 University of Plymouth Research Theses 01 Research Theses Main Collection 2014 Comparative Demography and Life history Evolution of Plants Mbeau ache, Cyril http://hdl.handle.net/10026.1/3201 Plymouth University All content in PEARL is protected by copyright law. Author manuscripts are made available in accordance with publisher policies. Please cite only the published version using the details provided on the item record or document. In the absence of an open licence (e.g. Creative Commons), permissions for further reuse of content should be sought from the publisher or author. Copyright Statement This copy of the thesis has been supplied on the condition that anyone who consults it is understood to recognise that its copyright rests with its author and that no quotation from the thesis and no information derived from it may be published without the author’s prior consent. Title page Comparative Demography and Life history Evolution of Plants By Cyril Mbeau ache (10030310) A thesis submitted to Plymouth University in partial fulfillment for the degree of DOCTOR OF PHILOSOPHY School of Biological Sciences Plymouth University, UK August 2014 ii Comparative demography and life history evolution of plants Cyril Mbeau ache Abstract Explaining the origin and maintenance of biodiversity is a central goal in ecology and evolutionary biology. Some of the most important, theoretical explanations for this diversity centre on the evolution of life histories. Comparative studies on life history evolution, have received significant attention in the zoological literature, but have lagged in plants. Recent developments, however, have emphasised the value of comparative analysis of data for many species to test existing theories of life history evolution, as well as to provide the basis for developing additional or alternative theories.
    [Show full text]
  • Highlands Scrub Hypericum Hypericum Cumulicola (Small) P
    Highlands Scrub Hypericum Hypericum cumulicola (Small) P. Adams ypericum cumulicola is a rare species that is Federal Status: Endangered (January 21, 1987) endemic to the Lakes Wales Ridge in central Florida. Critical Habitat: None Designated HIt is only known from Polk and Highlands counties. Florida Status: Endangered Within those counties, it may occur in relatively large populations of hundreds or thousands of individuals. The Recovery Plan Status: Revision (May 18, 1999) scrub hypericum is threatened by habitat loss, isolation of Geographic Coverage: Rangewide populations, and fire suppression threats, factors which led to its listing as an endangered species on January 21, 1987. Measures being used to conserve this species include land Figure 1. County distribution of Highlands scrub acquisition and management. This account represents a revision of the existing re- covery plan for the Highlands scrub hypericum (FWS 1996). Description Hypericum cumulicola is a small, short-lived perennial herb reaching 20 to 70 cm in height. It is branched from the base and has a woody, fibrous root system. The stems are shorter and more numerous in winter and spring before reproductive stalks are differentiated. Usually there are three stems, but there can be as many as 17 stems on a healthy plant (Quintana-Ascencio and Morales-Hernández in press). During the reproductive season, all stems of mature individuals bear flowers and fruits. The leaves of H. cumulicola are opposite, simple, entire, and needle-like. Flowers are small, bisexual, and arranged in cymes. The calyx consists of five distinct sepals, while the corolla consists of five bright yellow petals shaped like the blades of a propeller.
    [Show full text]
  • What Is the Evidence That Invasive Species Are a Significant Contributor to the Decline Or Loss of Threatened Species? Philip D
    Invasive Species Systematic Review, March 2015 What is the evidence that invasive species are a significant contributor to the decline or loss of threatened species? Philip D. Roberts, Hilda Diaz-Soltero, David J. Hemming, Martin J. Parr, Richard H. Shaw, Nicola Wakefield, Holly J. Wright, Arne B.R. Witt www.cabi.org KNOWLEDGE FOR LIFE Contents Contents .................................................................................................................................. 1 Abstract .................................................................................................................................... 3 Keywords ................................................................................................................................. 4 Definitions ................................................................................................................................ 4 Background .............................................................................................................................. 5 Objective of the review ............................................................................................................ 7 The primary review question: ....................................................................................... 7 Secondary question 1: ................................................................................................. 7 Secondary question 2: ................................................................................................. 7 Methods
    [Show full text]
  • Florida Scrub Is a Plant Community Easily Recognized
    Florida Scrub Including Scrubby Flatwoods and Scrubby High Pine lorida scrub is a plant community easily recognized FNAI Global Rank: G2/G3 by the dominance of evergreen shrubs and frequent FNAI State Rank: S2 Fpatches of bare, white sand. With more than two Federally Listed Species in S. FL: 32 dozen threatened and endangered species dependent upon scrub, the entire community is itself endangered. Recovery State Listed Species in S. FL: 100 of the community and its associated plants and animals will depend upon land acquisition and effective land Florida scrub. Original photograph courtesy of The management. Nature Conservancy. Synonymy Florida scrub in its various phases has been called xeric scrub, sand scrub, big scrub, sand pine scrub, oak scrub, evergreen oak scrub, dune oak scrub, evergreen scrub forest, slash pine scrub, palmetto scrub, rosemary scrub, and rosemary bald. Florida scrubs may be classified as coastal or interior. Scrubs are often named by the dominant plant species, as in rosemary scrub, sand pine scrub, palmetto scrub, or oak scrub. Some authors have confused closed-canopy forests of sand pine trees with scrub. Scrubs that are very recent in origin, usually a result of mans activities, are called pioneer scrubs. Communities intermediate between scrub and pine flatwoods have been called dry or xeric flatwoods but now are referred to as scrubby flatwoods. Communities intermediate between scrub and high pine have been called southern ridge sandhills, hickory scrub, yellow sand scrub, turkey oak scrub, turkey oak barrens, and natural turkey oak barrens, but probably are best referred to as scrubby high pine.
    [Show full text]
  • Conservation Implications of Genetic Variation in Three Rare Species Endemic to Florida Rosemary Scrub
    Butler University Digital Commons @ Butler University Scholarship and Professional Work - LAS College of Liberal Arts & Sciences 11-1999 Conservation implications of genetic variation in three rare species endemic to Florida rosemary scrub Rebecca W. Dolan Butler University, [email protected] Rebecca Yahr Eric S. Menges Matthew Halfhill Follow this and additional works at: https://digitalcommons.butler.edu/facsch_papers Part of the Botany Commons, and the Forest Sciences Commons Recommended Citation Dolan, R.W., Yahr, R., Menges, E.S. & Halfhill, M.D. (1999). Conservation implications of genetic variation in three rare species endemic to Florida rosemary scrub. American Journal of Botany, 86 (11), pp. 1556-1562. Available from: http://digitalcommons.butler.edu/facsch_papers/105. This Article is brought to you for free and open access by the College of Liberal Arts & Sciences at Digital Commons @ Butler University. It has been accepted for inclusion in Scholarship and Professional Work - LAS by an authorized administrator of Digital Commons @ Butler University. For more information, please contact [email protected]. American Journal of Botany 86(11): 1556±1562. 1999. CONSERVATION IMPLICATIONS OF GENETIC VARIATION IN THREE RARE SPECIES ENDEMIC TO FLORIDA ROSEMARY SCRUB1 REBECCA W. D OLAN,2,5 REBECCA YAHR,3,6 ERIC S. MENGES,3 AND MATTHEW D. HALFHILL4,7 2Friesner Herbarium, Butler University, Indianapolis, Indiana 46208; 3Archbold Biological Station, P.O. Box 2057, Lake Placid, Florida 33862; and 4Department of Biological Sciences, Butler University, Indianapolis, Indiana 46208 Habitat conversion and ®re suppression during the last 50 yr have greatly reduced and altered Florida scrub vegetation, resulting in threats to the persistence of its unique ¯ora.
    [Show full text]
  • Download Download
    A FLORISTIC INVENTORY OF THE HOLMES AVENUE TRACT (HIGHLANDS COUNTY), FLORIDA, U.S.A. George J. Wilder Jean M. McCollom Naples Botanical Garden Natural Ecosystems 4820 Bayshore Drive 985 Sanctuary Road Naples, Florida 34112-7336, U.S.A. Naples, Florida 34120-4800, U.S.A. [email protected] [email protected] Nancy J. Bissett The Natives, Inc. 2929 JB Carter Road Davenport, Florida 33837, U.S.A. [email protected] ABSTRACT Documented presently as growing wild within the Holmes Avenue Tract (Highlands Co., Florida, U.S.A) are individuals of 106 families, 282 genera, 464 species, and 478 infrageneric taxa of vascular plants. Of the 478 infrageneric taxa documented presently, 386 (80.8%) are native and 36 (7.5%) are endemic to Florida. Herein, seven main kinds of habitats are recognized for the study area, and individual taxa inhabit one or more of those habitats. Twenty-four presently reported infrageneric taxa are listed as Endangered (18 taxa) or Threatened (6 taxa) in Florida. Based on Wunderlin et al. (2019), 36 species plus one variety are newly reported for Highlands County. RESUMEN Hay ejemplares documentados actualmente que crecen en la Holmes Avenue Tract (Highlands Co., Florida, U.S.A) de 106 familias, 282 géneros, 464 especies, y 478 taxa infragenéricos de plantas vasculares. De los 478 taxa infragenéricos documentados actualmente, 386 (80.8%) son nativos y 36 (7.5%) endémicos de Florida. Se reconocen siete tipos principales de hábitats en el área de estudio, y taxa individu- ales viven en uno o más de esos hábitats. Veinticuatro taxa infragenéricos de los citados ahora están listados como en Peligro (18 taxa) o Amenazados (6 taxa) en Florida.
    [Show full text]
  • Jewels of the Ridge 20 Imperiled Plants of the Lake Wales Ridge Jewelslwridge 3/12/07 11:50 AM Page 3
    JewelsLWRidge 3/12/07 11:50 AM Page 2 Jewels of the Ridge 20 imperiled plants of the Lake Wales Ridge JewelsLWRidge 3/12/07 11:50 AM Page 3 Photos: facing page, from top left – Bonamia grandiflora, Warea carteri, Clitoria fragrans, Dicerandra frutescens, Polygonella myriophylla, Liatris ohlingerae, Prunus geniculata, Warea amplexifolia, Chionanthus pygmaeus. This page, below – Eriogonum longifolium var. gnaphalifolium. Photos © Shirley Denton. By Carl Weekley Twenty of the 47 vascular plants Florida scrub, scrubby flatwoods and in firelanes and other listed in Florida as threatened (T) or disturbed sites. Its name refers to its supposed medicinal endangered (E) by the US Fish and properties in curing whitlow, the flaking off of the skin Wildlife Service occur on the ancient around fingernails. Flowers are minute and inconspicuous. sandy spine of the state known as the Although the specifics of papery whitlow-wort’s reproductive Lake Wales Ridge (LWR). Although the biology are poorly understood, its populations thrive come LWR comprises less than 1% of the land fire, flood or off-road vehicle. mass of the state, it encompasses over Sandlace (Polygonella myriophylla), another mat-forming 40% of its federally listed plants. All species, is also known as woody wireweed because, in 20 species are endemic to the Ridge’s pyrogenic xeric upland contrast to most Florida members of its genus, it is a long-lived ecosystems, including Florida rosemary scrub, scrubby sub-shrub with woody stems. While sandlace generally prefers flatwoods, oak-hickory scrub and sandhill. Lake Wales Ridge white sand habitats, it also occurs on yellow sands. With its T & Es include species with fascinating biogeographical needle-like leaves, dark twisting stems and profuse creamy pasts and unusual ways of ensuring their reproductive white flowers it has the stark beauty of a Japanese print.
    [Show full text]
  • References 510 8 3 7 6 5 6 Line, WNC 95
    OUP UNCORRECTED PROOF – FIRSTPROOFS, Wed Oct 23 2013, NEWGEN 509.1 R e f e r e n c e s 509.2 Abrahamson , W.G. 1980 . Demography and vegetative reproduction. Pages 89–106 in 509.3 O.T. Solbrig , editor. Demography and evolution in plant populations . University of 509.4 California Press , Berkeley, CA . 509.5 Abrams , M.D. 1992 . Fire and the development of oak forests. BioScience 42 : 346–353 . 509.6 Abrams , M.D. 1998 . Th e red maple paradox. BioScience 48 : 355–364 . 509.7 Abrams , M.D. 2003 . Where has all the white oak gone? BioScience 53 : 927–939 . 509.8 Abrams , M.D. , and D.I. Dickmann . 1982 . Early revegetation of clear-cut and burned jack 509.9 pine sites in northern lower Michigan. Canadian Journal of Botany 60 : 946–954 . 509.10 Adams , M.S. 1970 . Adaptations of Aplectrum hyemale to the environment: Eff ects of pre- 509.11 conditioning temperature on net photosynthesis. Bulletin of the Torrey Botanical Club 509.12 97 : 219–224 . 509.13 Adams , V.M. , D.M. Marsh , and J.S. Knox . 2005 . Importance of the seed bank for popu- 509.14 lation viability and population monitoring in a threatened wetland herb . Biological 509.15 Conservation 124 : 425–436 . 509.16 Adkison , G.P. , and S.K. Gleeson . 2004 . Forest understory vegetation along a productivity 509.17 gradient . Journal of the Torrey Botanical Society 131 : 32–44 . 509.18 Adler , P.B. , et al. 2011 . Productivity is a poor predictor of plant species richness . Science 509.19 333 : 1750–1753 509.20 Aerts , R.
    [Show full text]
  • Section I Notices of Development of Proposed Rules and Negotiated
    Florida Administrative Weekly Volume 26, Number 29, July 21, 2000 Section I IF REQUESTED IN WRITING AND NOT DEEMED Notices of Development of Proposed Rules UNNECESSARY BY THE AGENCY HEAD, A RULE DEVELOPMENT WORKSHOP WILL BE HELD AT THE and Negotiated Rulemaking TIME, DATE AND PLACE SHOWN BELOW: TIME AND DATE: 9:00 a.m., August 11, 2000 DEPARTMENT OF STATE PLACE: Room 116, Larson Building, 200 East Gaines Street, Division of Historical Resources Tallahassee, Florida RULE CHAPTER TITLE: RULE CHAPTER NO.: THE PERSON TO BE CONTACTED REGARDING THE Historical Museums Grants-in-Aid 1A-43 PROPOSED RULE DEVELOPMENT AND A COPY OF PURPOSE AND EFFECT: The purpose and effect of the THE PRELIMINARY DRAFT, IF AVAILABLE, IS: James proposed changes is to amend the applications incorporated Goodloe, Chief, Bureau of Fire Prevention, Division of State into this rule and create new applications to differentiate Fire Marshal, Department of Insurance, phone (850)413-3620 between operational grants and program specific grants and to Pursuant to the provisions of the Americans with Disabilities obtain more information for each application to streamline Act, any person requiring special accommodations to application review procedures. participate in this program, please advise the Department at SUBJECT AREA TO BE ADDRESSED: The Bureau of least 5 calendar days before the program by contacting Yvonne Historical Museums proposes to amend the applications White at (850)413-4214. incorporated into this rule and create new applications to THE PRELIMINARY TEXT OF THE PROPOSED RULE differentiate between operational grants and program specific DEVELOPMENT IS NOT AVAILABLE. grants and to obtain more information for each application to streamline application review procedures.
    [Show full text]