International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Chairman Message Dear friends and colleagues, on behalf of the organizing committee, I take great pride in welcoming all the participants to the International Congress of Isfahan Biomedical Sciences (ICIBS-2020). The ICIBS would provide a great opportunity for biomedical scientists to present their cutting edge research. I hope this scientific event would provide a platform for scientists and students around the world to gather and share their research findings that would benefit the whole community. We believe that our first job is create a social atmosphere so that the participants could build their network that will eventually lead to the future collaborative projects. The Congress being held in glorious city of Isfahan. The whole city is represented as a museum of art, architecture and handicrafts. Isfahan is city of Abu Ali Al- Hussein Ibn Abdullah Ibn Sina, known in the West as Avicenna, who was the most eminent physician and philosopher of his days whose influence on the medicine persisted for centuries. Isfahan is historically intermingled with medical sciences and this will inspire us to get together, build and discuss modern ideas and concepts of Biomedical Sciences in a city that was once among the prominent leaders in medical sciences in the world. Hope a very joyful and fruitful stay for all of the participants
Professor Rasoul Salehi Chairman ICIBS 2020
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Congress Organization
ICIBS-2020 Chairperson Prof. Rasoul Salehi Head of Department of Genetic & Molecular Biology, Isfahan University of Medical Sciences
ICIBS-2020 Scientific Secretary
Dr. Mohammadreza Sharifi PhD, Professor (Associate) in the Department of
Genetics and Molecular biology, Isfahan University of
Medical Sciences
ICIBS-2020 Executive Secretary
Dr. Mehrdad Zeinalian MD, PhD, Professor Assistant in the Department of
Genetics and Molecular biology, Isfahan University of
Medical Sciences
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Scientific Committee
Prof. Fathia Mami-Chouaib Prof. Bahram Nasr Esfahani Dr. Naeimeh Tayebi Prof. Shaghayegh Haghjooy Javanmard Prof. Iscia Lopes-Cendes Prof. Peyman Adibi Prof. Khosrow Adeli Prof. Roya Kelishadi Dr. Zahida Qamri Prof. Parham Reisi Dr. Behrad Noudoost Prof. Abbas Rezaei Dr. Samar Sayedyahosein Prof. Masih Saboori Dr. Zahir Bhuiyan Prof. Shahnaz Razavi Dr. Neda Nategh Prof. Rasoul Salehi Dr. Maryam Azimzadeh Irani Prof. Mansoor Salehi Dr. Ali Rashidi-Nezhad Dr. Parvaneh Nikpour Dr. Babak Shojaie Dr. Hossein Khan Ahmad Dr. Majid Mojarrad Dr. Yousof Gheisari Prof. Mohammad Hossein Nasr- Esfahani Dr. Mohammad Amin Tabatabaiefar Prof. Seyed Mehdi Kalantar Dr. Mohammadreza Sharifi Prof. Kamran Ghaedi Dr. Mehrdad Zeinalian Prof. Tahereh Changiz Dr. Mohammad Kazemi Prof. Mehdi Nematbakhsh Dr. Rana Moradian Prof. Mohammad Hasan Emami Dr. Sharifeh Khosravi
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Day 1: 26th September / Morning
Opening
Time Program 8:15 - 8:30 Quran and National Anthem Prof. Tahereh Changiz 8:30 - 9 Chancellor of Isfahan University of Medical Sciences Prof. Rasoul Salehi, Chairman of ICIBS 2020 9 - 9:30 Director of Pediatric Inherited Diseases Research Center, Head of the Department of Genetics and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences Key Presenter: Prof. Shaghayegh Haghjoo Javanmard 9:30 - 9:55 Vice chancellor of research Isfahan University of Medical Sciences Tumor microenvironment Key Presenter: Dr. Naeimeh Tayebi 9:55 - 10:20 Washington University in Saint Louis School of Medicine, USA., USA High throughput technologies to investigate the molecular basis of congenital limb malformation 10.20 - 10.35 Break, Advertising Key Presenter: Prof. Iscia Lopes-Cendes 10.35 – 11:00 School of Medical Sciences,University of Campinad-Unicamp, Brazil Precision medicine: From definition to implementation Key Presenter: Prof. Roya Kelishadi Head of the Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan 11:00 – 11:25 University of Medical Sciences, Isfahan, Iran Epigenetics and the developmental origins of health and disease in the process of non-communicable diseases Key Presenter: Prof. Khosrow Adeli 11:25 – 11:50 Head and professor, Clinical Biochemistry, The Hospital for Sick Children, University of Toronto, Canada Postprandial dyslipidemia in obesity & insulin resistance and type 2 diabetes. Key Presenter: Dr. Zahida Qamri 11:50 – 12:15 University Comprehensive Cancer Center, Columbus-Ohio, USA Biomedical research: ethics, public trust and responsibilities of researchers
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Day 1: 26th September / Evening
Time Program Mehrdad Ostadpoor 14:00 – 14:10 Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran Comprehensive review of cinnamon’s effects on cancerous tumors Shirin Abdolvand School of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran 14:10 – 14:20 Altered expression of miR-146a and miR-148a as new biomarkers in healthy stomach tissues to distinguish the susceptibility of men and women to gastric cancer Mehnoosh Ashja-Arvan School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 14:20 – 14:30 Assessment of ability of human adipose derived stem cells for long term overexpression of IFN-β and LIF as therapeutic cytokines Azadeh Rahimi 14:30 – 14:40 School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran A novel homozygous ALS2 splice site variant causes a motor neuron disease in an Iranian family Bahare Zarin 14:40 – 14:50 Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran Mir-30 a master regulator that affects angiogenesis and stemness in melanoma tumor Banafsheh Torkan Cellular, Molecular and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran. 14:50 – 15:00 Faculty of Science, University of Isfahan. Anti-cancer effects of methanol Cordia myxa extract on caspase-3 in a breast cancer cell line Sara Anajafi 15:00 – 15:10 School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Detection of cancer biomarkers by microfluidic immunohistochemistry 15:10 – 15:25 Break, Advertising Mohammad Abdolvand Medical Genetics Research Center of Genome, Isfahan University of Medical Sciences, Isfahan, Iran 15:25 – 15:35 Expression Analysis of Has-mir-629-5p in Cancerous Tissues from Iranian Patients with Colorectal Cancer Maryam Zarin Semnan University of Medical Sciences, Semnan, Iran 15:35 – 15:45 MicroRNA Binding Site Polymorphism in Inflammatory Genes Associated with Colorectal Cancer: Literature Review and Bioinformatics Analysis Zoya Najafi 15:45 – 15:55 Science and Research branch, Islamic Azad University, Tehran, Iran Treatment with microRNAs in hepatocellular carcinoma Mostafa Saghi Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran 15:55 – 16:05 A scientific journey in circulatory miRNAs; Promising diagnostic and prognostic biomarkers in pancreatic cancer Samin Ghaderian 16:05 – 16:15 Shahid Sadoughi University of Medical Sciences, Yazd, Iran The evolving role of immune checkpoint inhibitors in ovarian cancer
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Day 2: 27th September / Morning
Medical Genetics and Bioinformatics and Systems Biology
8:15 – 8:30 Quran and National Anthem Key Presenter: Dr. Behrad Noudoost 8:30 – 8:55 Utah University, Utah, USA Prefrontal control of visual Signals Track 1 Medical Genetics Key Presenter: Prof. Mansoor Salehi Medical Genetics Research Center of Genome Isfahan, University of Medical Sciences, Isfahan, Iran 8:55 – 9:20 Medical School, Isfahan University of Medical Sciences, Isfahan, Iran Application of whole exome sequencing technique on 1000 Patients affected with different genetic diseases in Isfahan, Iran Akram Sarmadi Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of 9:20 – 9:45 Medical Sciences, Shahrekord, Iran Genetic linkage analysis of GCK and HNF1A genes in a group of families with MODY in Isfahan province Key Presenter: Dr. Zahra Kabiri 9:45 – 10:10 Duke University, US. Role of Wnt signaling in adult tissue homeostasis 10:15– 10:30 Break, Advertising Track 2 Bioinformatics and Systems Biology Akram Tajeddin Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran 10:30 –10:55 Bioinformatics identification of phagosome-related genes and pathways with potential to be used as biomarkers of early detection of ALS Dr. Fariba Dehghanian 10:55 - 11:20 Faculty of Biological Science and Technology, University of Isfahan, Iran New insights into structural systems biology Parnian Navabi Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 11:20 – 11:45 Effects of five different interchain linkers on spatial structures, stability and antigen-binding activities of the anti-CD25 scFv by bioinformatics studies
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Day 2: 27th September / Evening
Time Program Amir Safi Shahrekord University of Medical Sciences, Shahrekord, Iran 14:00 – 14:10 Different inhibitory activity of abemaciclib, hymenialdisine, and indirubin on CDK6: A molecular dynamics simulation Maryam Safaeipour Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran 14:10 – 14:20 Bioinformatics identification of markers related to neurodegeneration and mitochondrial dysfunction pathway in Amyotrophic Lateral Sclerosis Madine Mazaree Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of 14:20 – 14:30 Medical Sciences, Shahrekord, Iran LncRNAs role in of glioblastoma multiform carcinogenesis Iman Koosehlar Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran. 14:30 – 14:40 Bioinformatics evaluation of hsa-miR-1289 signaling pathways and its associated rs10277413 function in patients with non-small-cell lung cancer Seyedeh Mozhdeh Mirmohammadi Golestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Science, 14:40 – 14:50 Gorgan, Iran In silico pathway-based drug repurposing for HER2-positive subtype of breast cancer using drug database mining 15:10 – 15:25 Break, Advertising kolsoum InanlooRahatloo 15:25 – 15:35 School of Biology, College of science, University of Tehran, Iran Gene expression profile analysis during mouse tooth development Laleh Ebrahimi Ghahnavieh 15:35 – 15:45 Faculty of Biological Science and Technology, University of Isfahan, , Isfahan, Iran Emerging roles of the Hippo signaling pathway in non-small cell lung cancer: An In Silico study Key Presenter: Dr. Samar Sayedyahosein 15:45 – 16:10 Department of Anatomy & Cell Biology, University of Western Ontario, Canada Pannexin1 is a potential target for the treatment of malignant melanoma Farzaneh Ahmadi Shapoorabadi Faculty of Biological Science and Technology, Shahid Ashrafi Esfahan University, Isfahan, Iran 16:10 – 16:25 Bioinformatics evaluation of hsa-miR-542-3p signaling pathways and its associated rs3088012 function in patients with chronic lymphoid leukemia Fatemeh Kheiri Faculty of Basic Science, Shahrekord University, Shahrekord, Iran 16:35 – 16:45 Comparative study of the binding affinity of natural compounds for the prevention and treatment of breast cancer using molecular docking Zahra Sadri 16:55 – 17:05 Ala Cancer Prevention and Control Center, Isfahan, Iran Thymoma association with myasthenia gravis and molecular biomarkers: A case report Ali Zohoor Payame Noor University, Tehran, Iran 17:05 – 17:15 Identification of relationship between athero-miRs with the ribosome biogenesis pathway in recurrent acute coronary syndrome by transcriptome analysis
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Day 3: 28th September / Morning
Regenerative Medicine and Cell Therapy
Time Program 8:15 - 8:30 Quran and National Anthem Key Presenter: Dr. Mohammad Amin Tabatabaiefar 8:30 – 8:55 Isfahan University of Medical Sciences, Iran Molecular diagnostic of hearing impairment in Iran: Over a decade of experience Track 1 Regenerative Medicine Ahmad Reza Farmani 8:55 - 9:20 Tissue Engineering and Applied Cell Sciences Center, Tehran University of Medical Sciences, Tehran, Iran An overview of biological effects and clinical application of tumor treating fields in oncology Fereshteh Nejaddehbashi Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, 9:20 - 9:45 Khuzestan, Iran An electro spun mat composed of collagen and polycaprolactone loaded with low concentration of silver sulfadiazine can promote wound healing in rats 9:45 - 10:00 Break, Advertising Key Presenter: Dr. Zahir Bhuiyan Senior Lecturer and Privât Docent, Faculty of Biology and Medicine, University of Lausanne & University 10:00 – 10:25 Hospital Lausanne, Switzerland Genetics of cardiac arrhythmias Track 2 Cell Therapy Samira Asgharzadeh Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of 10:25 – 10:50 Medical Sciences, Shahrekord, Iran The differentiation of mesenchymal stem cells into photoreceptor like cells under the influence of the temporary increase of miR-96 expression Hoda Fazaeli Academic Center for Education, Culture and Research (ACECR), Qom Branch, Iran 10:50 – 11:15 Isolation and characterization of secreted exosomes from adipose tissue- derived mesenchymal stem cells Kaveh Khazaeel Department of basic sciences, Faculty of veterinary medicine, Shahid chamran university of Ahvaz, Iran 11:15 – 11:40 Graphene quantum dots (GQDs) and Fibrin Hydrogel Scaffold Can Induce Osteogenic Differentiation in Wharton’s Jelly derived Mesenchymal Stem Cells Mostafa Saghi Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran 11:40 – 12:05 Differentially expressed genes and molecular pathways in intellectual disability patients with mutation in Polr3b gene
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Day 3: 28th September / Evening
Time Program Sharareh Salmanizadeh 14:00 – 14:10 Cellular, Molecular and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran Amniotic membrane stem cells (AMSc): New perspectives for cancer cell therapies Elaheh Amirani Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran 14:10 – 14:20 The effects of selenium and probiotics co-supplementation on glycemic status and PPAR-γ gene expression in patients with gestational diabetes mellitus: a randomized, double-blind, placebo- controlled trial Mohsen Rezaei Aghdam 14:20 – 14:30 Faculty of Basic Science, Higher Education Institute of Rab-Rashid, Tabriz, Iran Stem cells as effective carriers for viral therapy in prostate cancer Nayereh Abdali 14:30 – 14:40 Faculty of Modern Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran The effect of different long non-coding RNAs on MDR1 drug resistance gene in cancer Fatemeh Shahi Sadrabadi Payame Noor University, Tehran, Iran 14:40 – 14:50 L- Carnitine and fibrin encapsulation effects on restoration of estrous cycle and ovarian function after auto transplantation in mice Akram Sarmadi School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 14:50 – 15:00 PedRo : Pedigree drawing software in Persian language to facilitate the process of documenting patients in medical centers with the ability to export (.pro) file 15:00 – 15:15 Break, Advertising Sara Soleimani Department of Cell and Molecular Biology, Faculty of Basic Sciences and Advanced Technologies in 15:15 – 15:25 Biology, University of Science and culture, Tehran, Iran Static magnetic field improves post vitrification quality of mouse GV oocytes Parisa Mohammadi Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran; 15:25 – 15:35 Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran Effect of oral B complex vitamin on sperm parameters and chromatin integrity in animal models of varicocele Amin Izaditabar Medical Genetics Research Center of Genome, University of Medical Sciences, Isfahan, Iran 15:35 – 15:45 Study of aneuploidies using karyotype, FISH and QF-PCR methods in amniotic fluid samples of 1500 pregnant women in Isfahan, Iran Atena Abed 15:55 – 16:05 School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran Ethical issues in stem cell research and treatment Amir reza Moghaddam 16:05 – 16:15 Department of paramedics, University of Danesh Alborz, Qazvin, Iran A novel dual thermo‑ and pH‑responsive silver nanocomposite hydrogel as a drug delivery system
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Day 4: 29th September / Morning
Cancer Diagnosis and Treatment
8:15 - 8:30 Quran and National Anthem Key Presenter: Prof. Kamran Ghaedi 8:30 - 9 Biological Science and Technology, Isfahan University, Iran Metabolic Aspects of Irisin in human health and biomedical regenerative medicine Track 1 Cancer Diagnosis Parisa Maleki Dana Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical 9 - 9:25 Sciences, Kashan, Iran PIWI-interacting RNAs as novel biomarkers for diagnosis and treatment of breast cancer Ali Shaker Khatibi 9:25 - 9:50 Faculty of basic sciences, Higher education institute of rabe rashid, Tabriz, Iran Circulating tumor cells in the context of personalized medical: Diagnosis and treatment of cancer Somaye Zeinalzadeh Faculty of Basic Sciences, Islamic Azad University, Kazerun, Iran 9:50 - 10:15 DNA methylation as diagnostic and prognostic biomarkers in colorectal cancer; Focus on blood strategies 10:15 – 10:30 Break, Advertising Key Presenter: Dr. Neda Nategh 10:30 – 10:55 Utah University, Utah, USA Toward understanding the brain computations creating our robust vision during free viewing Track 2 Cancer Treatment Tooba Abdizadeh Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences 10:55 – 11:20 Molecular docking evaluation of some analogues of biarylbenzamides as HDAC1 inhibitors for cancer treatment Fereshteh Mogheri Institute of Higher Education Rabe Rashidi Higher Education Institute. Tabriz. Iran. 11:20 – 11:45 Co-delivery of metformin and Silibinin with biocompatible PLGA–PEG copolymer nanoparticles for the treatment of non-small lung cancer Sara Norouzi Dizaj Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, 11:45 – 12:10 Iran Evolution of the expression levels of mir-132 in MCF-7 breast cancer cells treated with metformin loaded PLGA-PEG NPs, possible relationship with hTERT expression
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Day 4: 29th September / Evening
Time Program Forough Eidi Khosh 14:00 – 14:10 Faculty of Basic Sciences, Islamic Azad University, Dezful Branch, Dezful, Iran Association of rs6065668 C> T polymorphism in Tox2 gene with risk of colorectal cancer Mahsa Rastegar Moghaddam Mashhad Branch, Islamic Azad University, Mashhad, Iran 14:10 – 14:20 Effects of hydrophobic force on secondary structure of the most effective peptide in Alzheimer's disease Nasrin Zare Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran 14:20 – 14:30 Comparison of expression levels of hsa-miR-155, INPP5D and IFN-γ level in NK cells isolated from refractory/relapsed or responsive patients with large diffuse B-cell lymphoma (DLBCL) and healthy donors Farnoosh Attari 14:30 – 14:40 School of Biology, College of Science, University of Tehran, Tehran, Iran To overcome drug resistance in MDA-MB-231 breast cancer cells via paclitaxel loaded nanoemulsion Safoura Ghalamkari 14:40 – 15:50 Isfahan University of Medical Sciences, Isfahan, Iran MD simulations of L551Q as a novel carcinogenic PI3Kα mutation Fatemeh Kazemi 14:50 – 15:00 Islamic Azad University of Najaf Abad, Najaf Abad, Iran The role of wild-type p53 in cancer 15:00 – 15:15 Break, Advertising Sadra Salehi-Mazandarani Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 15:15 – 15:25 Bioinformatics analyses reveal differential expression and potential function of circular RNAs as efficient microRNA sponges in gastric cancer Nastaran Bagheri Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, 15:25 – 15:35 Isfahan, Iran Evaluation and comparison of C.524G>A mutation detection in TP53 gene by two methods of COLD- PCR/HRM and PCR-Sequencing in different dilutions of DNA mutations Nayereh Abdali 15:35 – 15:45 Faculty of Modern Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran The effect of different long non-coding RNAs on MDR1 drug resistance gene in cancer Mansour Homayoun School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 15:45 – 15:55 Evaluation of Grape seed extract (GSE) on PTEN and DACT1 tumor suppressor genes expression, cell cycle and apoptosis in ovarian cancer cell line (OVCAR-3) in vitro Mohammad Fakhrolmobasheri School of Medicine, Isfahan University of Medical sciences, Isfahan, Iran 15:55 – 16:05 Selenium supplementation can relieve the clinical complications of COVID-19 and other similar viral infections Milad Saadatkish School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences 16:05 – 16:15 Molecular Aspects of immunomodulatory and antioxidant effects of vitamin D on prevention of COVID-19 complications Mahboobeh Golchin Faculty of Basic Science, University of Maragheh, Maragheh, Iran 16:15 – 16:25 Knock down of SIRLNT by Antisense LNA GapmeRs and evaluation of its inhibitory effect on proliferation in human breast cancer cells
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Day 5: 30th September / Morning
Reproductive Biology and Biotechnology and Genetics Engineering
Time Program 8:15 - 8:30 Quran and National Anthem Key Presenter: Prof. Mohammad Hossein Nasr Esfahani 8:30 – 8:55 Royan Institute for Biotechnology, ACECR, Isfahan, Iran Effect sperm DNA mutations on the health of next generation Track 1 Reproductive Biology Niloofar Hassani Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and culture, Tehran, 8:55 - 9:20 Iran The effect of static magnetic field on mouse pre-antral follicle growth and matured oocyte fertilization in vitro Key Presenter: Prof. Seyed Mehdi Kalantar 9:20 - 9:45 Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran The role of Personal Medicine in Reproductive Medicine Niloofar Khajedehi Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and culture, Tehran, 9:45 - 10:10 Iran Mice oocyte vitrification does not influence fertilization by DNA fragmented sperms but reduces blastocyst formation 10:10 – 10:25 Break, Advertising Key Presenter: Dr. Maryam Azimzadeh Irani 10:25 – 10:50 Shahid Beheshti University, Tehran, Iran Unravelling the structural role of glycoconjugates in triggering the inflammatory response Track 1 Biotechnology and Genetics Engineering Nasim Hassani Faculty of Science, Tabriz branch Islamic Azad University, Tabriz, Iran 10:50 – 11:15 Synergistic anticancer effect of Nano-encapsulated metformin-artemisinin on grow and cell cycle arrest in human breast cancer T47D cell line. Mehdi Hassani 11:20 – 11:45 Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran BCL11A enhancer knock-out: An approach for the treatment of β-thalassemia Akram Mohammadi Faculty of Science, Tabriz Branch- Islamic Azad University, Tabriz, Iran 11:45 – 12:10 Design and synthesis of polymer particles containing Curcumin on hTERT gene expression in the lung breast cell line (MCF-7)
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Day 5: 30th September / Evening
Time Program Akram Mohammadi 14:00 – 14:10 Faculty of Science, Tabriz Branch- Islamic Azad University, Tabriz, Iran Macrophage repolarization using quercetin-based electrospun nanofibers: possible application in regenerative medicine Zoha Kamali 14:10 – 14:20 Isfahan University of Medical Sciences, Isfahan, Iran Linking cancer genomics to metabolites: A mendelian randomization study Sima Jafarpour 14:20 – 14:30 School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Non-invasive colorectal cancer screening by ITGA4 promoter methylation in peripheral blood mononuclear cells Fatemeh sadat Moravej Payame Noor University, Isfahan, Iran 14:30 – 14:40 The study of the protective effect of vitamin E and Retinoic acid on testicular tissue in mice treated with cyclophosphamide Mehri Nazeri Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical 14:40 – 14:50 Sciences, Kermanshah, Iran Importance of Circulating free DNA as a prognostic and predictive biomarker for recurrent miscarriage in women with subclinical hypothyroidism Maryam Ranjbar 14:50 – 15:00 Genetics department, Shiraz University of Medical Sciences, Shiraz, Iran Serial dilutions: A simple and accurate method to achieve genetically homogenous clones from a cell population Arezou Dabiri Pediatric Inherited Diseases Research Center, School of Medicine, Isfahan University of Medical Sciences, 15:00 – 15:10 Isfahan, Iran Investigating expression of SOX12 and its inhibition effect on induction of apoptosis in human Acute Myeloid Leukemia cell line (KG-1) by Antisense GapmeRs. 15:10 – 15:25 Break, Advertising Elham Naji Zavareh 15:25 – 15:35 Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Effect of cerium oxide nanoparticles on the expression of inflammatory cytokines in experimental autoimmune arthritis Abbasali Eskandarian School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 15:35 – 15:45 Knocking down the expression of the molecular motors; myosin A, C and F genes in Toxoplasma gondii (RH Strain) to decrease the parasite survival and virulence Asma Vafadar 15:45 – 15:55 School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran The effect of decitabine on the expression and methylation of the BTG2 in association with changes in miR-125b in NALM6 cell line Saeed Bagheri Mohammadi 15:55 – 16:05 School of Medicine, Medical Sciences of Shahid Beheshti University, Tehran, Iran Mesenchymal stem cells therapy exerts neuroprotection in mice model of Parkinson's disease Leila Naserpoor The Academic Center for Education, Culture and Research, Qom Branch, Qom, Iran 16:05 – 16:15 Association of rs1342945 and rs12478601 single nucleotide polymorphisms with Polycystic Ovarian Syndrome (PCOS): a case control study and in silico analysis Fatemeh Sadoughi Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, 16:15 – 16:25 Kashan, Iran Drug delivery and pancreatic cancer: are chitosan-based nanoformulations proper options ? Zoya Najafi 16:25 – 16:35 Isfahan University of Medical Sciences. Isfahan, Iran Study of chromosomal rearrangement in leukemia patients using Karyotype and FISH methods Shima Parviz 16:35 – 16:45 Department of Biology, Faculty of Sciences, Persian Gulf University, Bushehr, Iran
Sequencing of Exon.4 of the LDL Receptor Gene in Patients with Familial Hypercholesterolemia Nafiseh Mozafarian
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Day 6: 1th October/ Morning & Evening
Miscellaneous, Epigenetics Modification and Human Diseases
Time Program 8:15 – 8:30 Quran and National Anthem Key Presenter: Dr. Yousof Gheisari 8:30 – 8:55 Regenerative Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran A systems approach to kidney regeneration Track 1 Epigenetics Modification and Human Diseases Negin Badihian Child Growth and Development Research Center Research Institute for Primordial Prevention of Non- 8:55 – 9:20 Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran The epigenetic mechanisms associated with sleep during childhood Seyed Erfan Majidi 9:20 – 9:45 Isfahan University of Medical Sciences, Isfahan, Iran The role of epigenetics in Myopia: A review Key Presenter: Dr. Ali Rashidi-Nezhad 9:45 – 10:10 Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran Analysis of Genomic imbalances in patients with congenital anomalies 10:10 – 10:25 Break, Advertising Key Presenter: Dr. Babak Shojaie 10:25 – 10:50 St.-Marien Stift Medical Campus, Friesoythe, Germany The role of screening, organization and planning orthopedic surgeries during COVID-19 Track 2 Miscellaneous Mohammad Fakhrolmobasheri 10:50 - 11:15 School of Medicine, Isfahan University of Medical sciences, Isfahan, Iran A molecular perspective of Carnitine in the context of COVID-19 Elaheh Amirani Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran 11:15 – 11: 40 The effects of omega-3 fatty acids from flaxseed oil on inflammatory markers and gene expression related to inflammation and insulin metabolism in patients with gestational diabetes mellitus: a randomized, double- blind, placebo-controlled trial Ali Samadi Cellular and Molecular Research center, Ahvaz Jundishapour University of Medical Sciences, Ahvaz, Khuzestan, 11:40 – 12:05 Iran. Evaluation of the properties of the wound dressing film of chitosan / polyvinyl alcohol-based containing human adipose-derived stem cell exosomes, Silver nanoparticles and sildenafil citrate Shima Rahimirad School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 12:05 – 12:30 Genetically engineered mesenchymal stem cells: targeted delivery of immunomodulatory agents for tumor eradication Seyyed Saleh Hashemi 12:30 – 12:55 Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran Application of whole exome sequencing (WES) in clinical diagnosis; An example 12:55 – 13:20 Lunch Break Expert Panel of discussion on International Scientific Networking 13:20 – 15 Prof. Rasoul Salehi, Prof. Morteza Hashemzadeh chaleshtori, Prof. Peyman Adibi, Prof. Sayed Ahmad Vaez, Dr. Mehrdad Zeinalian 15 – 15:20 Break, Advertising Key Presenter: Prof. Fathia Mami-Chouaib 15:20 – 15:45 Head of Molecular Immunology Department, Gustave Roussy Hospital, Paris University, Paris, France Therapeutic cancer vaccines: Where are we now?
Key Presenter: Dr. Majid Mojarrad Department of Medical Genetics and Molecular Medicine, School of Medicine, Mashhad University of Medical 15:45 – 16:10 Sciences, Mashhad, Iran 12 Editing of dystrophin gene, a way to treatment of duchenne muscular dystrophy 16:30 Ending ceremony Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Content Precision medicine: From definition to implementation ______1
Epigenetics and the developmental origins of health and disease in the process of non-communicable diseases ______2
Postprandial dyslipidemia in obesity & insulin resistance and type 2 diabetes ______3
Application of whole exome sequencing technique on 1000 Patients affected with Different Genetic Diseases in Isfahan, Iran ______4
A direct Pannexin1 is a potential target for the treatment of malignant melanoma ______5
Genetics of cardiac arrhythmias ______6
Metabolic Aspects of Irisin in human health and biomedical regenerative medicine______7
The role of Personal Medicine in Reproductive Medicine ______8
Therapeutic cancer vaccines: Where are we now? ______9
Comprehensive59T Review of Stem Cell Effects on the Treatment of Diabetes type I 59T ______11
Altered expression of miR-146a and miR-148a as new biomarkers in healthy Stomach tissues to distinguish the susceptibility of Men and women to gastric cancer ______12
Assessment of ability of human adipose derived stem cells for long term overexpression of IFN-β and LIF as therapeutic cytokines ______13
A Novel homozygous ALS2 splice site variant causes a motor neuron disease in an Iranian family _ 14
Mir-30 a master regulator that affects angiogenesis and stemness in melanoma tumor ______15
Anti-cancer effects of methanolic Cordia myxa extract on caspase-3 in breast cancer cell line _____ 16
Detection of cancer biomarkers by Microfluidic Immunohistochemistry ______17
Expression Analysis of Has-mir-629-5p in Cancerous Tissues from Iranian Patients with Colorectal Cancer ______18
MicroRNA Binding Site Polymorphism in Inflammatory Genes Associated with Colorectal Cancer: Literature Review and Bioinformatics Analysis. ______19
Treatment with microRNAs in hepatocellular carcinoma ______20
A scientific journey in circulatory miRNAs; Promising diagnostic and prognostic biomarkers in 14 pancreatic cancer ______21 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
The Evolving Role of Immune Checkpoint Inhibitors in Ovarian Cancer ______22
Genetic linkage analysis of GCK and HNF1A genes in a group of families with MODY in Isfahan province ______23
Bioinformatics identification of phagosome-related genes and pathways with potential to be used as biomarkers of early detection of ALS ______24
New insights into structural systems biology ______25
Effects of five different interchain linkers on spatial structures, stability, and antigen-binding activities of the anti-CD25 scFv by bioinformatics studies ______26
Different Inhibitory Activity of Abemaciclib, Hymenialdisine, and Indirubin on CDK6: A Molecular Dynamics Simulation ______27
Bioinformatics identification of miRNAs associated to Amyotrophic Lateral Sclerosis ______28
LncRNAs Role in of Glioblastoma Multiform Carcinogenesis ______29
Bioinformatics evaluation of hsa-miR-1289 signaling pathways and its associated rs10277413 function in patients with Non-small-cell lung cancer ______30
In silico pathway-based drug repurposing for HER2-positive subtype of breast cancer using drug database mining ______31
Gene expression profile analysis during mouse tooth development ______32
Emerging roles of the Hippo signaling pathway in non-small cell lung cancer: An In Silico study __ 33
Bioinformatics evaluation of hsa-miR-542-3p signaling pathways and its associated rs3088012 function in patients with chronic myeloid leukemia. ______34
Comparative study of the binding affinity of natural compounds for the prevention and treatment of breast cancer using molecular docking. ______35
Thymoma, association with myasthenia gravis and molecular biomarkers: A case report ______36
Identification of relationship between athero-miRs with the ribosome biogenesis pathway in recurrent acute coronary syndrome by transcriptome analysis ______37
An Overview of Biological Effects and Clinical Application of Tumor Treating Fields in Oncology _ 38
An Electrospun Mat Composed of Collagen and Polycaprolactone Loaded with Low Concentration of
Silver Sulfadiazine Can Promote Wound Healing in Rats ______39 15 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
The differentiation of mesenchymal stem cells into photoreceptor like cells under the influence of the temporary increase of miR-96 expression ______40
Isolation and characterization of secreted exosomes from adipose tissue- derived mesenchymal stem cells ______41
Graphene quantum dots (GQDs) and Fibrin Hydrogel Scaffold Can Induce Osteogenic Differentiation in Wharton’s Jelly derived Mesenchymal Stem Cells ______42
Differentially expressed genes and molecular pathways in Intellectual disability patients with mutation in Polr3b gene ______43
Amniotic Membrane Stem Cells (AMSc): new perspectives for cancer cell therapies ______44
The effects of selenium and probiotics co-supplementation on glycemic status and PPAR-γ gene expression in patients with gestational diabetes mellitus: a randomized, double-blind, placebo-controlled trial ______45
Stem cells as effective carriers for viral therapy in prostate cancer ______46
The effect of different long non-coding RNAs on MDR1 drug resistance gene in cancer ______47
L59T - Carnitine and fibrin encapsulation effects on restoration of estrous cycle and ovarian function after
autotransplantation in mice59T ______48
PedRo: Pedigree drawing software in Persian language to facilitate the process of documenting patient’s information in medical centers with the ability to export (.pro) format ______49
Static magnetic field improves post vitrification quality of mouse GV oocytes ______50
Effect of oral B complex vitamin on sperm parameters and chromatin integrity in animal models of varicocele ______51
Study of aneuploidies using karyotype, FISH and QF-PCR methods in amniotic fluid samples of 1500 pregnant women in Isfahan, Iran ______52
Ethical Issues in Stem Cell Research and Treatment ______53
A novel dual thermo‑ and pH‑responsive silver nanocomposite hydrogel as a drug delivery system _ 54
PIWI-interacting RNAs as novel biomarkers for diagnosis and treatment of breast cancer ______55
Circulating Tumor Cells in the context of Personalized Medical: Diagnosis and Treatment of Cancer56
DNA Methylation as Diagnostic and Prognostic Biomarkers in Colorectal Cancer; Focus on Blood 16 Strategies ______57 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Molecular docking evaluation of some analogues of biarylbenzamides as HDAC1 inhibitors for cancer treatment ______58
Co-delivery of metformin and Silibinin with biocompatible PLGA–PEG copolymer nanoparticles for the treatment of non-small lung cancer ______59
Evolution of the expression levels of mir-132 in MCF-7 breast cancer cells treated with Metformin loaded PLGA-PEG NPs, Possible relationship with hTERT expression ______60
Association of rs6065668 C> T polymorphism in Tox2 gene with risk of colorectal cancer ______61
Effects of hydrophobic force on secondary structure of the most effective peptide in Alzheimer's disease ______62
Comparison of expression levels of hsa-miR-155, INPP5D and IFN-γ level in NK cells isolated from refractory/relapsed or responsive patients with large diffuse B-cell lymphoma (DLBCL) and healthy donors ______63
To overcome drug resistance in MDA-MB-231 breast cancer cells via paclitaxel loaded nanoemulsion ______64
MD simulations of L551Q as a novel carcinogenic PI3Kα mutation ______65
The role of wild-type p53 in cancer ______66
Bioinformatics analyses reveal differential expression and potential function of circular RNAs as efficient microRNA sponges in gastric cancer ______67
Evaluation and comparison of C.524G>A mutation detection in TP53 gene by two methods of COLD- PCR/HRM and PCR-Sequencing in different dilutions of DNA mutations ______68
Evaluation of Grape seed extract (GSE) on PTEN and DACT1 tumor suppressor genes expression, cell cycle and apoptosis in ovarian cancer cell line (OVCAR-3) in vitro ______69
Selenium supplementation can relieve the clinical complications of COVID-19 and other similar viral infections ______70
Molecular Aspects of immunomodulatory and antioxidant effects of vitamin D on prevention of COVID- 19 complications ______71
Knock down of SIRLNT by Antisense LNA GapmeRs and evaluation of its inhibitory effect on proliferation in human breast cancer cells ______72
The effect of static magnetic field on mouse pre-antral follicle growth and matured oocyte fertilization 17 in vitro ______73 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Mice oocyte vitrification does not influence fertilization by DNA fragmented sperms but reduces blastocyst formation ______74
Synergistic anticancer effect of Nano-encapsulated metformin-artemisinin on grows and cell cycle arrest in human breast cancer T47D cell line ______75
BCL11A enhancer Knock-out: an approach for the treatment of β-thalassemia ______76
Design and synthesis of polymer particles containing Curcumin on hTERT gene expression in the lung breast cell line (MCF-7) ______77
Macrophage repolarization using quercetin-based electrospun nanofibers: possible application in regenerative medicine ______78
Linking cancer genomics to metabolites: A Mendelian randomization study ______79
Non-invasive colorectal cancer screening by ITGA4 promoter methylation in peripheral blood mononuclear cells ______80
The study of the protective effect of vitamin E and Retinoic acid on testicular tissue in mice treated with cyclophosphamide ______81
Importance of Circulating free DNA as a prognostic and predictive biomarker for recurrent miscarriage in women with subclinical hypothyroidism ______82
Serial dilutions: A simple and accurate method to achieve genetically homogenous clones from a cell population ______83
Investigating59T expression of SOX12 and its inhibition effect on induction of apoptosis in human Acute
Myeloid Leukemia cell line (KG-1) by Antisense GapmeRs.59T ______84
Effect of cerium oxide nanoparticles on the expression of inflammatory cytokines in experimental autoimmune arthritis ______85
The Effect of Decitabine on the Expression and Methylation of the BTG2 in Association with Changes in miR-125b in NALM6 Cell Line ______86
Mesenchymal stem cells therapy exerts neuroprotection in mice model of Parkinson's disease _____ 87
Association of rs1342945 and rs12478601 single nucleotide polymorphisms with Polycystic Ovarian Syndrome (PCOS): a case control study and in silico analysis ______88
The potential role of chitosan-based nanoparticles as drug delivery systems in pancreatic cancer ___ 89
18 Study of chromosomal rearrangement in leukemia patients using Karyotype and FISH methods ___ 90 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Sequencing59T of exon.4 of the LDL Receptor gene in patients with Familial Hypercholesterolemia with
the aim of studying possible new mutations 59T ______91
Application of propensity score in the relationship of watching TV and computer and metabolic syndrome in adolescents: the CASPIAN-III Study ______92
The epigenetic mechanisms associated with sleep during childhood______93
The Role of Epigenetics in Myopia: A Review ______94
A molecular perspective of Carnitine, in the context of COVID-19 ______95
The effects of n-3 fatty acids from flaxseed oil on inflammatory markers and gene expression related to inflammation and insulin metabolism in patients with gestational diabetes mellitus: a randomized, double-blind, placebo-controlled trial ______96
Evaluation of the properties of the wound dressing film of chitosan / polyvinyl alcohol-based containing human adipose-derived stem cell exosomes, Silver nanoparticles and sildenafil citrate ______97
Genetically engineered mesenchymal stem cells: targeted delivery of immunomodulatory agents for tumor eradication ______98
Application of whole exome sequencing (WES) in clinical diagnosis; An example ______99
Blastocoel Fluid Biopsy As A Non-invasive Preimplantation Genetic Testing Method ______100
The application of magnetic particle imaging in targeted cancer treatments ______102
Evaluation of changes in enzymes of MDA, GPX, SOD, catalase and hepatocytes of male offspring of vitamin E treated rats ______103
Circulating cell-free DNA as a prognostic and predictive biomarker for IVF outcome in Polycystic Ovary Syndrome females ______104
Analysis of the ligand transport pathway through a eukaryotic transporter using Caver Web server 105
Application of Piezoelectric nanobiomaterials in regenerative medicine and cancer therapy ______106
Designing a potent epitope based vaccine against brucellosis using bioinformatics approaches ____ 107
Bioinformatics strategy for Determination of microRNAs and Genes Involved in Pathogenesis of Aneurysm in Patient with Autosomal Dominant Polycystic Kidney Disease (ADPKD) ______108
Evaluation of expression of CDR1, CDR2 genes in clinical isolates of Candida albicans resistant and
susceptible to fluconazole in patients under chemotherapy in Ahvaz ______109 19 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Effect of hydroalcoholic and aqueous extract of Dracocephalum kotschyi on belomycin pulmonary fibrosis ______110
A review of the role and effects of miRNAs in the diagnosis and prevention of diabetes ______111i
Determination of deleterious SNPs of PIK3CA gene and their impacts on the structure of PI3K ___ 112
Bioinformatics study of single nucleotide polymorphism rs1800469 and hsa-miR-22-5p related to it in Acute Lymphoblastic Leukemia ______113
Statistical analysis of insect anticancer peptides ______114
Effects of intraction of Orexin B Antagonist on the Fatty Acid Synthase Enzymes Genes Expresstions of Milk Lipid in the Lactating Rats ______115
MicroRNAs as novel Potential biomarker in gastric cancer: Diagnostic and prognostic biomarker 116
Folate supplementation's effect on epidemiology of Colorectal Cancer ______117
Study of the influence of raster angle on the mechanical properties of 3D printed Polycaprolactone / 45S5 Bioglass® scaffolds ______118
in silico study of association of rs35613052 with hsa-miR-770-5p in acute leukemia ______119
Mesenchymal stem cells as a therapeutic method for COVID-19 ______120
A study of probability distribution of the single nucleotide variants of rs7579 and rs34713741 inSEPP1 and SELs genes of the colorectal cancer patients compared with a control group of random patients in Isfahan AL-zahra hospital ______121
Bioinformatics study of single nucleotide polymorphism rs2070672 and its association hsa-mir-199a-5p in patients with acute lymphoblastic leukemia ______122
Novel therapeutic approaches against metastatic melanoma cells ______123
Role of Magnesium in Cancer Treatment ______124
miRNA-mRNA Regulatory Networks of Some Neurotransmitter Receptors in Nervous System Disease ______125
Co-incidence of ductal breast cancer and gastrointestinal cancers: A case-series study ______126
The Effect Anticancer Characteristics of the Alcoholic Extract of Dracocephalum kotschyi in the Breast Cancer Cell Line ______127
Recent strategies for noninvasive glucose monitoring ______128 20 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
In silico molecular docking of IDH1 r132 mutant inhibitors as anti-cancer agents: a comparative study ______129
Early-phase diagnosis of plasma miRNA-21 in cancerous patients based on time and intensity of color changing in gold nanoparticles ______130
Metabolomics in cancer biomarker discovery; new trend in personalized medicine for breast cancer 131
Comparative evaluation of 3D polycaprolactone scaffolds fabricated by 3D printing and salt leaching methods ______132
Risk of Infertility and celiac disease; a case report and literature review ______133
Valuable methods to identify therapeutic target pathways in cancers: CRISPR screening and RNA seq ______134
microRNAs roles on apoptosis in pancreatic cancer ______135
Early diagnosis of breast cancer using tumor-derived exosomes ______136
Mohammad Ghasemi1, Leyla Norouzi-Barough2, Sadegh Shirian1* ______136
The study of clinical findings and chronological age in Arab horses in association with telomerase expression ______137
Association between Toll-Like Receptor 4 and Response to Chemotherapy drugs in Breast Cancer 138
Association between Toll-Like Receptor 4 and Response to Chemotherapy drugs in Breast Cancer 139
Bioinformatic designing multi-epitope protein of muc1 against cancer ______140
Antioxidant, antimicrobial and antitumor activity of Bacteriocins of Gram-Positive Bacteria _____ 141
Chicken chorioallantoic membrane as an effective animal modeling for bio molecular studies ____ 142
The growth inhibitory capacity of Thymus daenensis Celak essential oil on MDA-MB-231 cancer cells ______143
Evaluation the biodistribution of RGD-modified nanoliposomes containing curcumin in rats by HPLC ______144
Isolation and the genetic study of antibiotic-resistant Escherichia coli strains in the urine of patients referred to Ahvaz Veterans Clinic ______145
Synthesis and Evaluation of Nanofibrous Scaffolds containing bioactive Silibinin on the expression of interleukin-2 in mesenchymal stem cells: possible application in spinal cord injury repair ______146 21 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Does selenium affect the expression of programmed cell death protein and its ligand in colorectal cancer? ______147
Investigation Chondrogenesis of hASCs On The [DCM/ PVA/ Fibrin] Hybrid Scaffolds: In vivo study ______148
Key signaling pathways in papillary thyroid carcinoma ______149
Nisin: The Antimicrobial and Anticancer Properties ______150
Fabrication of nano-biocomposite of poly (lactide-co-glycolide)/chitosan electrospun scaffold for transdifferentiation of Schwann-like cells from human adipose-derived stem cells ______151
Enrichment of differentially expressed eRNAs in cancer-related pathways in gastric cancer ______152
Epigenetic markers; a review of promising milieu for personalized diagnosis and treatment of osteoporosis ______153
Association between genetic markers of PCOS1 region and polycystic ovarian syndrome ______154
The effect of gassericin A on the gene expression of tumor Necrosis Factor alpha (TNF-α) in differentiated preadipocyte 3T3-L1 cells ______155
Extraction and Investigation some of Biological Characteristics of Venom Rhopilema nomadica in the Persian Gulf ______156
Comprehensive transcriptomic analysis reveals a relation between expression alteration of transcribed ultraconserved regions (T-UCR) and hypoxia ______157
Hypoxia-targeted therapies for patients with nonsmall cell lung carcinoma ______158
Inhibition of MDA-MB-231 cancer cell proliferation by Crocus cancellatus subsp. damascenus corm methanol extract ______159
Single Step Smart Sensing of Proteinmarkers Using DNA Strand Displacement and Split G-Quadruplex Enhanced Fluorescence ______160
Effect of Cartilage ECM on polycaprolactone/Fibrin hybrid scaffold fabricated by salt leaching method ______161
The effect of Platelet Rich Plasma on proliferation and survival of Adipose –Derived Stem Cells in Fibrin and Alginate scaffolds ______162
Laser Therapy ______163
22 The Effect of Estrogen in Diffuse Axonal Injury: A Randomized Clinical Trial ______164 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Immunomodulatory effects of Cinnamomum against COVID-19 ______165
Isolation of methicillin-resistant Staphylococcus aureus from urine in patients referred to Ahvaz Pasteur Laboratory ______166
The59T Association of Anemia with Learning and Memory in Students of Imam Raouf Educational
Complex of Isfahan, 1397 59T ______167
Wound healing effects of ZnO nanocomposite containing Alchemilla extract in rats ______168
Overview of microRNA targeting ______169
Bioinformatics evaluation of targetomhas-miR-143-3p signaling pathways and related function ofHIF- 1α, SNP(rs11549465) in patients with acute lymphoblastic leukemia ______170
Molecular mechanisms of the anti-inflammatory effects of thymus vulgaris L. in COVID-19 patients ______171
Studying the effects of essential oil and extract of Teucrium Polium on cell proliferation of Gastric Adenocarsinoma Cell lines ______172
Bioinformatics assessment of PPARγ role in regulation of gene networks expression involved in congenital heart disease ______173
Detection of novel mitochondrial mutations in cytochrome C oxidase subunit 1 (COX1) in patients with familial adenomatous polyposis (FAP) ______174
Construction of Multi-Component Scaffold Consisting of DNA Building Blocks and Aptamer Base on DNA Self-Assembly ______175
The effect of licorice extract on prostate volume and its clinical symptoms in patients with benign prostatic hyperplasia (BPH) ______176
Epi-miRNAs: A Subclass of MiRNAs in Cancer ______177
A novel dual thermo‑ and pH‑responsive supermagnetic iron oxide nanocomposite hydrogel as controlled release of anticancer drug ______178
Mutation of Caspase Cleavage Site in XIAP and its Effect on Interaction ______179
Comparison of Two Methods of Ice-COLD HRM-PCR and Full-COLD HRM-PCR in Detection of E542K Mutation in PIK3CA gene in Breast Cancer ______180
Characterization and Development of Skin Allograft Scaffolds as a Biological Dermal Substitute for
23 Wound Healing ______181 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Evaluation of the effect of ECM on poly caprolactone/Fibrin hybrid scaffold fabricated by 3D Printing method ______182
Retinal Precursor-Like Cells Differentiation of Human Adipose-Derived Stem Cells by Hanging Drop Culture ______183
Review on the main types of eukaryotic and prokaryotic promoters and a glimpse of promoter engineering ______184
PBMCs: A New Source of Diagnostic and Prognostic Biomarkers ______185
Anti-tumor effects of NDV in a female Doberman with lymphoma cancer ______186
Skin Mast Cell Promotion in Random Skin Flaps in Rats using Bone Marrow Mesenchymal Stem Cells and Amniotic Membrane ______187
Farzaneh Chehelcheraghi1*, Leila Zarei2 ______187
Recent advances on aptamer-based biosensors to detection of prostate specific antigen (PSA) ____ 188
The CRISPR Approach to Molecular Systems Biology and Personalized Medicine ______189
The effects of Microbiota on cancer ______190
Study of Antidepressant Drugs Effect on Development of the Brain ______191
Circular RNAs as promising tools for cancer therapy ______192
Tracking of stem cells ______193
From antimicrobial peptide to anticancer peptide ______194
Genetic variation in circadian rhythm gene CLOCK with susceptibility to multiple sclerosis ______195
Effect of Glaucium Flavum Extract on Bax and Bcl2 Genes Expression in HT-29 Cancer Cells __ 196
Insight to nano-curcumin interaction with alpha crystallin: spectroscopy and molecular docking study ______197
Nutrigenomics studies and Evaluation of Irisin level Affected by the Expression of Skeletal Muscle Genes ______198
SiRNA Bioinformatics Design Against mRNA of N, NSP12, NSP16, NSP5 Genes to Prevent mRNA Translation and Sars_cov_2 Virus Replication ______199
Molecular identification of Staphylococcus epidermidis and Staphylococcus aureus in children and
24 adults suspected of sepsis in Rafsanjan city ______200 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Anticancer activity of methanolic Cordia myxa extract on caspase-8 in breast cancer ______201
A review on role and function of REST (restrictive element-1 silencing transcription factor) in neurodegenerative diseases ______202
Mechanisms of Ataxia Pathogenesis and Role of Genetic Factors ______203
Glaucium Flavum Induction Autophagy in HT-29 Cancer Cells ______204
Association of rs4073 with IL-8 activation by transcription factor and its role in angiogenesis, diffusion of immature white blood cell and acute lymphoblastic leukemia by bioinformatics analysis ______205
MBD1 Expression and its correlation with Clinicopathological Prognostic Factors ______206
The effect of Moringa olifera leaf extract on blood glucose in diabetic rats ______207
Bioanformatic study of effective microRNAs on growth factors of cancer pathway ______208
Sulfur mustard gas and epigenetic modifications in skin cancer: A Review ______209
The investigation of the interaction between piperine and human serum albumin compared with holo transferrin nutural protein by both spectroscopic and synchronous fluorescence technique for cancer prevention ______210
The role of BACE1-AS and BC200 long non coding RNAs in Alzheimer’s disease ______211
Initial characterization of human skin stem cells ______212
TEAD regulation and its impact on Cancer inhibition. ______213
The Effects of Mesenchymal Stem Cells on Tumors and Cancers ______214
Unravel common comprising miRNAs in various types of cancers using comprehensive data-mining. ______215
Coenzyme Q10 and its therapeutic potencies against COVID-19 and other similar infections: A molecular review ______216
Evaluation of immunomodulatory effect of Dracocephalum kotschyi extract on immune system in animal model ______217
Genomic instruments help to find Driver nodes in co-expression networks ______218
Determination of Antioxidant Potential, Phenolic Content, Flavonoid Content and Antitumor Effect of
Trachyspermum ammi seed Essential Oil on Breast Cancer Cell lines MDA-MB-231, MDA-MB-468, Mcf7 ______219 25 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Bioinformatics study of single nucleotide polymorphism rs3733890 and hsa-miR-185-5p related to it in Acute Lymphoblastic Leukemia ______220
Biological concepts and potential clinical implications of piRNA in cancer ______221
The role of lncRNAs in Hepatocellular carcinoma ______222
Preplasmic expression of a tandem scvf antibody for simultaneous targeting of two immune check-points ______223
Design and evaluation of two novel chimeric proteins, p28-IL-24 and p28-M4, targeted to breast cancer cells: An In-Silico approach ______224
Modern dressings in the treatment of diabetic ulcer ______225
Silybum Marianum for Wound Healing ______226
Host-Directed Drug Therapies for leishmaniasis ______227
Bioinformatics evaluation of has-miR-514a-3p associated with single nucleotide polymorphism (rs143847362) TET2 gene and myeloid cancer. ______228
Expression changes of miR-29c-3p in glucose-affected mesenchymal stem cells ______229
The Effect of Decitabine on the Expression and Methylation of the PPP1CA in Association with Changes in miR-181b in NALM6 Cell Line ______230
Losartan alleviates kidney fibrosis via downregulation of collagen IV in rats with unilateral ureteral obstruction ______231
Cell-free DNA: Fast tool for diagnosis ______232
Effect of flaxseed and chamomile extract on concentration of some biochemical serum factors in rats with experimental gastric ulcer ______233
Evaluation of single nucleotide mutation in exon 1 of myoc gene in patients with primary congenital glaucoma ______234
The role of LncRNAs in endometriosis ______235
Bioinformatics study of single nucleotide polymorphism rs2279744 and hsa-miR-10a-3p related to it in Acute Lymphoblastic Leukemia ______236
Study59T of cytotoxic of Glaucium Flavum Extract on HT-29 Cancer Cells59T ______237
Insilico study of bioactive compounds having similar structure to cell death inducer natural products in 26 Cancer ______238 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
The effect of piascledine and TGF β1 on chondrogenesis of human adipose-derived stem cells in fibrin and fibrin-alginate scaffolds ______239
Oral Administration of Probiotic Enterococcus Durans Ameliorates Experimental Autoimmune Encephalomyelitis in Mice ______240
Examination of the role of genetics in thyroid neoplasm ______241
Surface Modification of Polycaprolactone Scaffold via Immobilized Carboxymethyl Chitosan for Bone Tissue Engineering Application ______242
Reconstruction59T of the co-expression network for the hippo signaling pathway in glioblastoma 59T ____ 243
Bioinformatics study of the binding affinity and pharmacological properties of anticancer compounds targeting topoisomeraseI in cancer treatment. ______244
A large deletion of the BRCA1 gene in a hereditary breast cancer: A case report ______245
Evaluation of the role of rs3789327 polymorphism in increased risk of multiple sclerosis ______246
Bioinformatics study of hsa-miR-99a and LNCRNA ANRIL targeting pathways in acute lymphoblastic leukemia ______247
S14161 as a novel small molecule promoted the apoptosis in A549 cancer cells ______248
Evaluation of GSK3β gene expression alteration during adipogenesis in human mesenchymal stem cells ______249
Stem cells treatment for HIV ______250
Synthesis and fabrication of quercetin-loaded electrospun nanofibrous mats with antioxidant activity for proliferation and stemness preservation of human mesenchymal stem cells ______251
Warton's jelly explant is a source of EGF and bFGF growth factors ______252
In59T Silico Molecular Docking of HSPA2 with 4HNE in Human sperm59T ______253
Metformin- mesoporous silica nanoparticles loaded electrospun thermo-responsive copolymer for targeted ovarian cancer therapy ______254
Investigation of the myelin recovery and expression changes of miR-219 and miR-155-3p following the administration of hydroxychloroquine in multiple sclerosis-induced model ______255
Cervical ectopic pregnancy: one cause of first trimester pregnancy bleeding (a case report) ______256
Copper compounds as anti-colorectal cancer agents ______257 27
Page Association of Protease Enzymes with Cancer ______258
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Omega 3 acid and wound healing in diabetes ______259
The effects of addition of vitamin B12 in semen cryopreservation medium on the post-thaw sperm function ______260
Investigating of cyanidin binding behavior upon interaction with human serum albumin and human transferrin: biophysical and molecular docking approaches ______261
Peripheral Blood or Bone Marrow stem cells? ______262
How arginine crosses across the prokaryotic and eukaryotic membranes: a mechanistic study by umbrella sampling approach ______263
Advanced targeted therapy in cancer: nanostructured carriers as innovative tools for cancer diagnosis and therapy ______264
Effect of inhibition of miR-21 on lncRNA of tumor suppressor gene role in MCF-7 cells ______265
Intravascular brachytherapy with Iridium 192 dosimetry ______266
An implantable smart electrospun nanofibers with switchable drug release for hyperthermic tumor cell treatment ______267
Chronic myeloid leukemia and Klein Filters Syndrome______268
The Significance of GPC1+ circulating exosomes in early detection of Pancreatic Cancer ______269
Circular RNAs (circRNAs) as novel diagnostic biomarkers for different diseases ______270
Interaction between apolipoprotein B insertion/deletion polymorphisms and macronutrient intake with obesity risk, blood lipids, serum leptin and ghrelin in type 2 diabetes mellitus patients ______271
A review of Electrospun Nanofiber Poly L-Lactic Acid scaffold application for angiogenesis in biomedical researches ______272
Bioinformatics study of fatty acid ester in fungus ______273
Annexin A1: as novel biomarkers for screening, diagnosis, and monitoring of female reproductive system cancer ______274
Application59T of 3D Bio-Printing in Regenerative medicine and Tissue Engineering 59T ______275
Co-Culture of mesenchymal stem cells with endothelial progenitor cells inside alginate-gelatin microspheres promoted angiogenic potential ______276
Application of Various Stem Cells for Nerve Tissue Engineering ______277 28 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
The relationship between hot-wet temperament in Iranian Traditional Medicine (ITM) and Non- alcoholic Fatty Liver Disease (NAFLD) from systems biology point of view ______278
Bioinformatics evaluation of targetom has-miR-128-3p signaling pathways and related function of ALDH2, SNP (rs671) in patients withacute lymphoblastic leukemia ______279
The Effect of Dracocephalum Kotschyi Alcoholic Extract on the BAX Expression in SKBR3 Cell Line ______280
In silico Analysis of caffeine effects on NMDA receptor in Alzheimer’s disease ______281
A Bioinformatics approach to identify overlapped genes between SARS-COV-2 and lung cancer __ 282
An in silico approach to design a novel multi-epitope peptide vaccine against Helicobacter Pylori _ 283
Co-transplantation of human neurotrophic factors secreting cells and adipose-derived stem cells in rat model of MS ______284
The role of oral magnesium sulfate in the stimulation of PPAR-γ and inhibition of NFKB genes expression in improving hyperglycemia in the muscle of STZ-induced diabetic rat ______285
Oral mucosal mesenchymal stem cells ______286
Resveratrol and Gliomas: Where are we standing? ______287
The Impact of Environmental Contaminants on Telomere Length ______288
Effects of Rheum ribes on paraoxonase1 activity in hyperlipidemia rats ______289
Role of trehalose on structural and functional stability of D-lactate dehydrogenase enzyme ______290
Introducing New Inhibitors for Kallikrein-related peptidase 6 (KLK6) ______291
Interaction between nut consumption with TCF7L2 polymorphisms and weight gain on metabolic syndrome incidence ______292
Replacement in animal researches, a review article ______293
Evaluation59T of the effects of extremely low-frequency electromagnetic fields on the viability of human
gastric cancer (AGS) cell line 59T ______294
Association between cholesterol ester transfer protein polymorphisms and dyslipidemia in children and adolescents ______295
Design of Nano bio-sensor based on graphene oxide for detection of mutation F508del in CFTR gene, leading to Cystic fibrosis ______296 29
Page Scar-free skin healing ______297
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Gene therapy ______298
Hanging drop culture enhances differentiation of human adipose-derived stem cells into anterior neuroectodermal cells using small molecules. ______299
Medical Devices and Artificial Organs ______300
Molecular docking of five colon cancer drugs with the erbb1 receptor ______301
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Key Speaker: Iscia Lopes-Cendes ID Code:18942
Precision medicine: From definition to implementation
Iscia Lopes-Cendes
Professor of Medical Genetics and Precision Medicine, Department of Translational Medicine, School of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil e-mail: [email protected]
Abstract: Precision Medicine (PM) combines an array of different information, aiming to improve healthcare delivery. As PM uses individualized information from the patient, such as genomic signatures and medical history, it allows for more accurate diagnoses and treatment. It is a significant improvement over the current paradigm, in which physicians prescribe medicines designed for the average patient. However, we cannot implement PM without understanding the contribution of human genomic diversity to health and disease. Therefore, the development of PM strategies requires detailed knowledge of the genetic background of the population. Thus, large-scale genomic studies have been performed to characterize the genetic architecture of many population groups. More recently, national initiatives devoted to the implementation of PM are currently being developed in several countries. One issue which becomes critical as projects on PM are launched worldwide is the necessity of sharing genomic and health-related information. Thus, allowing for more rapid development of PM and improving our understanding of the mechanisms underlying diseases that affect individuals worldwide. Because the Brazilian population has a unique genomic structure, we created a network of scientists and clinicians devoted to a PM project, The Brazilian Initiative on Precision Medicine (BIPMed, www.bipmed.or). We believe that in addition to its local importance for the full implementation of PM in Brazil, we expect that BIPMed may pave the way to similar initiatives by other countries.
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Key Speaker: Roya Kelishadi ID Code: 96399
Epigenetics and the developmental origins of health and disease in the process of non-communicable diseases
Roya Kelishadi1*, Parnian Poursafa2 1. Department of Pediatrics, Child Growth and Development Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran 2. Department of Biology, University of Isfahan, Isfahan, Iran
Corresponding author: Prof. Roya Kelishadi, Department of Pediatrics, Child Growth and Development Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease.
Abstract: We present a comprehensive review on developmental origins of health and disease about different factors related to the origins of non-communicable diseases (NCD) from early life. The impacts of various factors including epigenetics; gene-environment interaction; ethnic predisposition to NCDs and their underlying risk factors; prenatal factors; fetal programming; maternal weight status and weight gain during pregnancy; type of feeding during infancy; growth pattern during childhood; obesity; stunting; socioeconomic status; dietary and physical activity habits; active, secondhand, and thirdhand smoking, as well as environmental factors including air pollution and global climate change on the development and progress of cardiovascular diseases and their risk factors. Growing body of evidence exists about Developmental Origins of Health and Disease (DOHaD). Maternal and paternal environmental factors, as well as the gene-environmental interactions act by different mechanisms by which the environment would model the epigenome: receptor signalling, energy metabolism and signal mechanotransduction from extracellular matrix to chromatin. The implication of epigenetics in DOHaD is well documented. The importance of early identification of predisposing factors for primordial and primary prevention of NCDs from early life should be highlighted. Keywords: Epigenetics; Non-communicable disease; Prevention
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Key Speaker: Khosrow Adeli ID Code: 33665
Postprandial dyslipidemia in obesity & insulin resistance and type 2 diabetes
Khosrow Adeli
Head and professor, Clinical Biochemistry, The Hospital for Sick Children, University of Toronto, Canada
Background: Obesity and insulin resistance, increasingly prevalent in adolescents, commonly associates with postprandial dyslipidemia, an independent predictor of cardiovascular disease risk. Postprandially gut peptides, glucagon-like peptide 1 (GLP-1) and GLP-2, modulate intestinal dietary fat absorption and triglyceride-rich lipoprotein (TRL) output. Bile acids are also postprandial factors implicated in lowering lipemia. We hypothesize that the postprandial response of GLP-1, GLP-2, and bile acids to a high-fat meal is impaired in obese, insulin resistant adolescents and associates with postprandial dyslipidemia. Methods: Normal weight (n=15), obese insulin sensitive (n=20), and obese insulin resistant (n=10) adolescents underwent an oral fat tolerance test (83% kcal from fat) with blood collected at 0, 1, 2, 4, and 6 hours. The lipoprotein phenotype was obtained by nuclear magnetic resonance spectroscopy, GLP-1 and GLP-2 were measured by ELISA and the bile acid profile was quantified by mass spectrometry. Continuous variables, including area under the curve (AUC) and incremental AUC (iAUC), were compared by one-way analysis of variance (ANOVA) or Kruskal-Wallis. Postprandial differences were also compared by two-way mixed ANOVA. Results: Obese, insulin resistant adolescents exhibited dyslipidemia, particularly reduced high-density lipoprotein particle size and exaggerated postprandial intestinally-derived large TRLs. Postprandial plasma levels of GLP-1 and GLP-2 were blunted in obese, insulin resistant subjects and inversely correlated with postprandial dyslipidemia. However, fasting GLP-1 and GLP-2 directly correlated with postprandial dyslipidemia, suggesting a compensatory increase in fasting secretion. Postprandial, but not fasting, total bile acids were diminished in obese adolescents and inversely correlated with insulin resistance and postprandial dyslipidemia. Specifically, postprandial lithocholic acid was reduced, a potent stimulator of GLP-1 secretion. Conclusion: Postprandial GLP-1, GLP-2, and bile acids were blunted in response to a high-fat meal in obese, insulin resistant adolescents. However, it remains unknown if these postprandial metabolic changes are a cause or consequence of impaired glucose and lipid metabolism in an obese state.
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Key Speaker: Mansour Salehi ID Code: 21126
Application of whole exome sequencing technique on 1000 Patients affected with Different Genetic Diseases in Isfahan, Iran
Mansour Salehi1,2, Mahdiyeh Behnam2 1. Division of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 2. Medical Genetics Research Center of Genome, Isfahan University of Medical Sciences, Isfahan, Iran
Purpose: Whole exome sequencing (WES) that was primarily developed for the research project application, has increasingly been used as a diagnostic tool in clinical medicine. Clearly in the next few years, WES will become accepted as a routine technology in the clinical genetics laboratory for disease diagnosis, prognosis and even treatment. In this paper we report results obtained from 1000 WES analysis. Methods: We performed WES for about 1000 affected patients referred to medical genetic center of Genome during 8-year period (2011– 2019) with a mean coverage of above 100X. Data from WES were analyzed for identification of single nucleotide variants (SNV) or copy number variants (CNV). Results: The most frequently genetic variants were identified in autosomal recessive causing genes (about 57.8%), much higher than autosomal dominant causing genes (7%) and X-linked (3.4%). Although, the majority of the disease causing variants located in nuclear genome, analyzing of mitochondrial genome variants in the WES data yielded six variants in MT-ATP6, MT-ND6, MT-ND5 and MT-TK genes. Interestingly 13 patients had copy number variants (CNV) more than 5 kb. Our analysis results show that less than 30% of the patients failed to obtained acceptable genetic diagnosis by WES-based technology. Diagnostic rate was dependent on the family history information and clinical findings of the affected individuals. Conclusion: Cost effectiveness of this high throughput sequencing has made it more feasible for clinical application. However, Interpretation of unknown significant variants, incidental findings, and novel variants needs more functional, computational evidences. Keywords: whole exome sequencing, patients, rare disease
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Key Speaker: Samar Sayedyahosein ID Code: 88578
A direct Pannexin1 is a potential target for the treatment of malignant melanoma
Samar Sayedyahosein Department of Anatomy & Cell Biology, University of Western Ontario, Canada
Introduction: Melanoma is the most aggressive type of skin malignancy with increasing incidence worldwide. Previously, we have shown that the channel forming protein, Pannexin1 (PANX1), regulates several cellular processes in melanoma cells including proliferation, migration and invasion/metastasis in vitro and ex vivo. However, the mechanism through which PANX1 mediates melanoma progression has not been elucidated. We have observed that knocking down PANX1 decreases the abundance of β-catenin, a key transcription factor in the Wnt signaling pathway. Wnt pathway regulates several processes in melanoma cells, including proliferation, migration and metastasis. These observations prompted us to evaluate the potential crosstalk between PANX1 and components of the Wnt signaling pathway. Hypothesis: We hypothesized that PANX1 modulates melanoma progression through crosstalk with the Wnt signaling pathway. Materials and Methods: In silico analysis was conducted to determine whether there is any correlation between PANX1 and β-catenin mRNA in samples from patients with malignant melanoma or breast carcinoma. We used immunoprecipitation studies, as well as in vitro analysis using pure proteins to evaluate the interaction of PANX1 with β-catenin, a key transcription factor in the Wnt signaling pathway. We used CRISPR/Cas9 system to generate PANX1 knock out cells. PANX1 and β-catenin knockdown cells were generated using shRNA and further used in the functional assays, such as proliferation and mitochondrial metabolic stress test. We used PANX1 blockers, Probenecid (PBN) and Spironolactone (SPL), to inhibit PANX1 channel function and determine their impact on the abundance and subcellular localization of β-catenin. Confocal microscopy was used to evaluate the subcellular localization of β-catenin in PANX1-deficient melanoma cells. We used RT-PCR to determine changes in the mRNA levels of Wnt pathway effector proteins, such as β-catenin, MITF and LEF1 in PANX1-deficient cells. Results: In this study, we demonstrated that PANX1 binds to β-catenin and modulates its subcellular localization. PANX1 and β-catenin co-immunoprecipitated from several established melanoma cell lines. The interaction of PANX1 β-catenin and has a functional impact on melanoma cells. In vitro analysis using pure proteins demonstrated a direct interaction between PANX1 and β-catenin. Analysis with fragments of PANX1 revealed that the association is mediated through the C-terminal region of PANX1. The abundance of β-catenin is significantly decreased when PANX1 is either knocked down or inhibited by two PANX1 blockers, Probenecid and Spironolactone. Fluorescence imaging showed a disrupted pattern of β-catenin localization at the cell membrane in PANX1-deficient cells. Several Wnt target genes, including Microphthalmia-Associated Transcription Factor (MITF), were significantly suppressed in PANX1-deficient cells. Both PANX1- and β- catenin-deficient melanoma cells grew significantly less than controls in culture. In addition, mitochondrial metabolism of PANX1-deficient cells was significantly impaired, indicating a role for PANX1 in the regulation of melanoma cell metabolic profile. Discussion and Conclusions: Here we report a previously undescribed role for PANX1 in regulation of the Wnt signaling pathway through direct interaction with β-catenin, a significant effector of the Wnt signaling. The
findings of our study provide mechanistic insight into PANX1-mediated melanoma progression and may be
5 applicable to other contexts where PANX1 and β-catenin interact as a potential new component in the Wnt signaling pathway. Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Key Speaker: Zahir Bhuiyan ID Code:63829
Genetics of cardiac arrhythmias
Zahir Bhuiyan
Senior Lecturer and Privât Docent, Faculty of Biology and Medicine, University of Lausanne & University Hospital Lausanne, Switzerland Abstract: Primary cardiac arrhythmias are inherited cardiac diseases. Genetic defects causal to the primary cardiac arrhythmias e.g. Long QT syndrome (LQTS), Catecholaminergic Polymorphic Ventricular Tachycardia (CPVT) are usually found in a gene that are responsible for cardiac action potential generation and eventual rhythmic contraction of the heart. Three most common genes associated with LQTS are KCNQ1, KCNH2, and SCN5A. In addition, CPVT causal genetic defects are found in Ca2+ handling genes e.g. RYR2, CASQ2, TECRL, CALM1, CALM2 and CALM3; clinical phenotype is usually severe. Mutations in CALM1, CALM2 and CALM3, though initially described in CPVT patients, but later they were found predominantly in LQTS patients with severe forms. Variability in clinical penetrance is observed mostly in carriers with a mutation in KCNQ1, KCNH2, and SCN5A genes. In 3-5% of patients, genetic defects could be found ≥ 2 genes, phenotype is usually severe in these patients. In addition, our study has also revealed that in some communities arrhythmia propensity could be severer due to recessive inheritance of the disease. Detection of a disease causal mutation facilitates in proper clinical diagnosis, which also leads to gene targeted therapeutic and behavioral management. Most importantly, mutation detection leads to cascade screening of the family members, where preventive measures could be implemented to prevent unwarranted sudden occurrence of ventricular fibrillation and/or sudden cardiac deaths. In my presentation, I will present different types of cardiac arrhythmias caused by defects in the aforementioned genes; pathophysiology of primary arrhythmias and their clinical management will be addressed. In particular, I will focus on a new type of hereditary malignant arrhythmia, CPVT3 (OMIM: 614021), which we have first reported in 2007.
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Key Speaker: Kamran Ghaedin ID Code:65637
Metabolic Aspects of Irisin in human health and biomedical regenerative medicine
Kamran Ghaedi
Biological Science and Technology, Isfahan University, Iran Abstract: In my laboratory, we have pinpointed functional aspects of Fndc5 which was recognized as a kind of myokine and could be cleaved and secrets as a hormone, termed Irisin with an important role in thermogenesis and energy homeostasis. Within one decade our colleagues were involved in unraveling the function and expression of FNDC5 in a number of cell types. We have chased the expression of Fndc5 during the neural differentiation of both mouse and human embryonic stem cells. On the other hand, we analyzed the different splicing variant form of Fndc5. Using gain/loss of function approach we indicated that Fndc5may play a prerequisite step in neural differentiation of embryonic stem cells as well as cardiac differentiation. Furthermore, we did an intensive search on the structure Fndc5 promoter. As the expression of Fndc5 up-regulated post retinoic acid treatment during neural differentiation of mouse embryonic stem cells. This interesting data motivated ass to check whether this enhancement is observable in neural differentiation of human embryonic stem cells. Therefore, results showed that all Fndc5 mRNA isoforms have a similar increasing pattern. Also, we explored that RA enhanced Fndc5 expression through a non-genomic pathway through the ERK signaling pathway. Interestingly, we identified that Fndc5 acts in PPARγ/PGC1-Fndc5 axis in cardiac differentiation of embryonic stem cells. Key words: Cardiac differentiation, Embryonic stem cells, Fndc5, Neural differentiation.
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Key Speaker: Seyed Mehdi Kalantar ID Code:12432
The role of Personal Medicine in Reproductive Medicine
Seyed Mehdi Kalantar
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Abstract: Personalized medicine is an exciting and powerful field, offering new tools to deliver better care to patients. The concept of personalized medicine is not new. It back to ancient times, Hippocrates combined an assessment of the four humours—blood, phlegm, yellow bile, and black bile—to determine the best course of treatment for each patient. Avicenna, an Iranian philosopher and physician of the tenth and eleventh centuries (4th and 5th century A.H.) in his book of Canon divided each individual in 4 groups as different temperaments: Cold, Warm, Dry and wet. This time, we know that polymorphisms in DNA sequence may lead to different expression of the encoded protein or to the expression of altered protein, and thus to a different health outcome in conventional medicine the people who suffering from the same disease (phenotype) are considered vs in personalized medicine each individual according to his or her genetic background should be considered the phenotype and treating. Personalized medicine is an exciting and powerful field, offering new tools to deliver better care to patients. in reproduction problem who has problem to have child either infertility problem or repeated pregnancy loss during nearly half century ART provided opportunity to treat the problem whoever, in some cases with less success. So in such cases need to consider for each individual to use different protocols e.g. donation, received the ovarian stimulation regime according to her genetic background, or looking some newly protocol as three partner cycle for some mtDNA abnormality were discussed. Key words: Personalized medicine, ARPL, mtDNA
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Key Speaker: Fathia Mami-Chouaib ID Code:97193
Therapeutic cancer vaccines: Where are we now?
Fathia Mami-Chouaib
Head of Molecular Immunology Department, Gustave Roussy Hospital, Paris University, Paris, France
Abstract: An effective antitumor immune response is often associated with the presence of T cells directed toward cancer neoepitopes, a class of major histocompatibility complex (MHC)- bound peptides that arise from tumor-specific mutations. However, tumors frequently use multiple mechanisms to evade cytotoxic T lymphocyte (CTL) recognition and destruction. Among these mechanisms, defects in genes relevant for antigen presentation by MHC class + I molecules to CD8 T lymphocytes, such as loss of β2m and alterations in transporter associated with antigen processing (TAP), play a major role by inducing a sharp decrease in surface expression of MHC-class I/β2m-peptide complexes. Therefore, developing therapeutic cancer vaccines based on tumor neoantigens that are selectively presented by cancer cells carrying defects in antigen processing and presentation was a major challenge over the last two decades. In this regard, we have previously identified a non-mutant tumor
neoepitope, ppCT16-25, derived from preprocalcitonin (ppCT) leader sequence and processed independently of proteasomes/TAP by a novel mechanism involving signal peptidase (SP) and signal peptide peptidase (SPP). We have recently shown that most human lung tumors display altered expression of TAP and frequently express ppCT self- antigen. ppCT includes several HLA-A2-restricted T-cell epitopes that are processed by proteasome/TAP-independent and -dependent pathways. We have also provided in vitro and in vivo proof of concept of an active immunotherapy based on ppCT-derived peptides capable of controlling growth of immune-escaped tumors expressing low levels of MHC- class I molecules. Non-mutant and mutant neoepitopes are promising targets for therapeutic cancer vaccines in combination with immune checkpoint inhibitors, such as anti-PD-1.
9 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 96392
Comprehensive Review of Stem Cell Effects on the T reatment of Diabetes type I
Mehrdad Ostadpoor 1*, Nasser Yazdani 1, Majid Gholami-Ahangaran 2, Seyyed Hossein Heidari 1 , Pooria Rezaei 1 1. Doctor of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran 2. Department of Poultry Diseases, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Introduction: Diabetes Mellitus (DM) is Chronic Disease and has Become a Prevalent Health Issue Globally. It is Estimated that 5% of all Deaths in the World Are Caused by Diabetes and the Number is Growing Rapidly. It is Characterized by the Immune Complex Mediated Attack on Insulin Producing B Cells of the Pancreas. Current Approaches Utilized for Pancreatic Replacement of Damaged β Cells Include Cadaveric Islet Transplantation, Induction of Endogenous Regeneration and Administration of Stem Cell- Derived β Cells . Description: In the Current Literature Review Keywords Including Diabetes Mellitus, Insulin, Hyperglycemia and Stem Cell from the List of MeSH and other Credible Scientific Websites such as Science Direct, PubMed and Google Scholar Were Used to Compile the Effects of stem Cell on Treatment of Diabetes Mellitus. Discussion and conclusion: Studies on Diabetic Nephropathy Showed that Intravenously Administered Bone Marrow- Mesenchymal Stem Cells (BM-MSCs) could Improve Insulin Secretion, Reduce the Expression of Inflammatory Markers and Reverse Hyperglycemia. In addition, it could Improve the Functional Parameters of Kidneys with Diabetic Nephropathy. Betacellulin (BTC), a Ligand of the Epidermal Growth Factor Receptor, Promotes the Growth and Differentiation of Pancreatic β Cells and Improves Glucose Metabolism in Experimental Diabetic Models. In the Diabetic Neuropathy Transplantation of Stem Cells Obtained from Murine Dental Pulp, Improved Pancreatic Damage and Renal Function was Observed. Moreover, Adipose-Derived- Mesenchymal Stem Cells (AD- MSC) Treatment could also Reverse Hyperglycemia and Ameliorate Autoimmune Diabetes Pathogenesis. Hematopoietic Stem Cell (HSCs) could also Increase β Cell Function, C-Peptide Synthesis and Decrease HbA1c Levels. As a Result, MSC-Induced Immunomodulation Gives Hope for the Development of Improved Diabetic Treatments through Stem Cell-Based Cell Therapy in the Future. Keywords: Diabetes Mellitus, Insulin, Hyperglycemia and Stem Cell
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 78153
Altered expression of miR-146a and miR-148a as new biomarkers in healthy Stomach tissues to distinguish the susceptibility of Men and women to gastric cancer
Shirin Abdolvand1, Mehdi Moghanibashi2*, Parisa Mohamadinejad3
1. M.sc Student in Genetics, Department of Genetics, School of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran 2. Assistant Professor, PhD in Molecular Genetics, Department of Genetics, School of Medicine, Kazerun Branch, Islamic Azad University, Kazerun, Iran 3. Assistant Professor, PhD in Cellular and Molecular Biology, Department of Biology, School of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Background: The incidence of gastric cancer is more common in men than women with ratio 2 to 1 in the Globe. One of the most important biological reasons is sexual hormones between two genders which in turn cause sexual dimorphism, leading a sex bias in incidence of disease between two sexes. MicroRNAs are involved in sexual dimorphism in genes expression. In this study, given the sex hormones response elements in the regulatory regions of miR-148a and miR-146a and sexual dimorphism in incidence of gastric cancer, for finding new biomarkers for prognosis and diagnosis, the expression of these two microRNAs in the stomach of healthy women and men at different age groups were compared. Materials and Methods: In this study, healthy tissues gained from gastric antrum of 35 healthy men and 35 healthy women. RNA contents were extracted and cDNA synthesised, the expression of miR-148a and miR-146a were compared between sexes by Real time RTPCR technique and data were analyzed by using ANOVA and independent sample T tests. Results: The expression of miR-148a and miR-146a did not show significant differences in two sexes. However, expression of miR-148a was more in men over 45 years than men under 45 years (p value< 0.05). Also, the expression of miR-146a was significantly high in men under 45 years than men over 45 years (p< 0.05). But, there was no significant difference between women over 45 years and women under 45 years. Conclusion: The expression of miR-148a and miR-146a with aging in men in the stomach increased and decreased respectively which suggest new biomarkers for prognosis and diagnosis of Gastric cancer. Keywords: MiR-148a, MiR-146a, Sexual dimorphism, Gastric cancer.
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 27781
Assessment of ability of human adipose derived stem cells for long term overexpression of IFN-β and LIF as therapeutic cytokines
Mehnoosh Ashja-Arvan1, Moein Dehbashi2, Asma Eslami1, Hossein Salehi3, Mahdiyeh Yoosefi4, Mazdak Ganjalikhani-Hakemi1*
1. Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. 2. Division of Genetics, Department of Biology, Faculty of Sciences, University of IsfahanIsfahan, Iran. 3. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Correspondence: *Mazdak Ganjalikhani-Hakemi, PhD, Associate Professor, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Introduction: Adipose-derived stem cells (ADSCs) are a subset of MSCs that their therapeutic effects in various diseases make them an interesting tool in cell therapy. ADSCs can be genetically engineered by lentiviral vectors for cell-based therapy purposes. Objectives: In the current study we aimed to overexpress IFN-β and LIF cytokines in human ADSCs and assess the ability of transduced ADSCs for long-term expression of IFN-β and LIF, simultaneously. Materials & Methods: Here, we designed a construct containing IFN-β and LIF and then, transduced characterized hADSCs by this construct via a lentiviral vector (PCDH-513B). We assessed the ability of long term expression of the transgene in transduced cells by western blotting and ELISA techniques on days 15, 45 and 75 after transduction. Results: According to the obtained data, 95.8% and 94.7% of isolated hADSCs cells were positive for expression of CD73 and CD105, respectively while the expression of CD45, as a negative marker, was only 5.85% and isolated hADSCs successfully differentiated into osteocytes and adipocytes. In addition, our results showed high-efficiency transduction with highest expression rates on day 75 after transduction which were 70 pg/ml for IFN-β and 77.9 pg/ml for LIF compared with 25.60 pg/ml and 27.63 pg/ml, respectively in un- transducted cells (p=0.0001). Conclusion: All in all, we successfully constructed a hADSC population stably overexpress IFN-β and LIF cytokines. Considering the IFN-β and LIF anti-inflammatory and neuroprotective effects as well as immune-regulatory properties of hADSCs, the obtained cells of this study could be subjected for further detailed evaluations of their impact on the EAE mice model. Keywords: Mesenchymal stem cells (MSCs); Adipose-derived stem cells (ADMSCs); Transduction; Stem cell; Overexpression
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 28258
A Novel homozygous ALS2 splice site variant causes a motor neuron disease in an Iranian family
Azadeh Rahimi 1, †, Mohammad Reza Pourreza 1, †, Javad Mohammadi-asl 2, Mohammad Amin Tabatabaiefar 1, 3, * 1. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. 2. Department of Medical Genetics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 3. Pediatric Inherited Diseases Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran. † These authors contributed equally to this work.
Introduction & Objectives: Recessive pathogenic variants in the alsin Rho guanine nucleotide exchange factor (ALS2) gene lead to three distinct neurodegenerative disorders comprising infantile-onset ascending hereditary spastic paralysis (IAHSP), juvenile primary lateral sclerosis (JPLS), and juvenile amyotrophic lateral sclerosis (JALS), which are early-onset motor neuron diseases with the upper motor neuron and/or the lower motor neuron involvement. The goal of this study is to identify the underlying genetic cause of a neuromuscular disease in a consanguineous Iranian family by applying whole-exome sequencing (WES). Materials & Methods: Clinical manifestations were surveyed in affected individuals of a consanguineous Iranian family with a neuromuscular disorder. We performed WES, bioinformatics analysis, Sanger validation and co-segregation analysis in this family to find the causative variant. Results: We report a novel ALS2 splice site variant co-segregating patients presenting with spastic tetraparesis, emotional lability, dysarthria, hyperreflexia and becoming wheelchair bound. This homozygous variant is predicted to affect the RCC1 (regulator of chromatin condensation)-like domain, encoding a putative guanine exchange factor for Ran guanosine triphosphatase, causing a loss of ALS2 function due to instability of the mutant protein. In silico evaluations confirmed the pathogenic effect of the variant. It was classified as pathogenic according to the ACMG variant interpretation guideline. Conclusions: This study highlights the importance of using WES as a powerful tool for constituting a quick and accurate genetic diagnosis of complex neuromuscular phenotypes. We recommend screening the ALS2 gene in Iranian cases with a family history of neuromuscular disorder. Keywords: ALS2; Alsin; motor neuron disease; whole-exome sequencing
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 66925
Mir-30 a master regulator that affects angiogenesis and stemness in melanoma tumor
Bahare Zarin1,2, Jahangir Noori1,2, Shaghayegh Haghjooy Javanmard1,2* 1. Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran 2. Department of physiology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Objectives: Mir-30 plays a dual role in tumor angiogenesis acting both as a suppressor and promoter of tumor vessel formation. Stemness profile almost often is negatively controlled by Mir-30 decreasing tumor progression. The aim of this study was the evaluation of mir- 30 transfection in melanoma tumor angiogenesis and stemness profile. Material and methods: MiR-30a-5p has been transfected into B16-F10 melanoma cells, then cells were injected subcutaneously in to C57BL/6 mice. Then the mice were sacrificed and the vessel density was evaluated by immunohistochemical staining procedure. The extent of nestin and oct-4 expression was evaluated by RTQ-PCR to assess stemness. Results: Vessel density significantly decreased in melanoma mouse models after transfection with miR-30a-5p when compared with control group and scramble as negative control. Mir-30a transfection significantly increased Nestin and oct-4 mRNA expression. Our results offer a significantly different explanation distinct from many other experimental observations suggesting overexpression of miR-30 in melanoma tumor cells increased their self-renewal ability and stemness profile. Conclusion: Overexpression of Mir-30 does not always correlate with desirable outcomes and its overexpression may result in tumor progression and poor prognosis due to increasing stemness state ensuring excess proliferation of tumor cells.
15 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 34339
Anti-cancer effects of methanolic Cordia myxa extract on caspase-3 in breast cancer cell line
Banafsheh Torkan1,2, Nasrin Hadi1,3, Fatemeh Ketabchi4, Farinaz Khosraviani1,3, Mansoor Salehi1,3,4 1. Cellular, Molecular and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran. 2. Department of Biology, faculty of science, University of Isfahan. 3. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. 4. Medical Genetics Research Center of Genome, Isfahan University of Medical Sciences, Isfahan, Iran.
Introduction: Breast cancer is one of the most common malignant diseases and the second cause of death among women in the world, and according to statistics, one in every 12 people suffers from breast cancer. Iranian patients with breast cancer are younger than their Western counterparts. Plant-based drugs are promising therapeutic agents against cancers. Cordia myxa is a plant of Boraginaceae family, widely cultivated in Iran. Thus, we assessed the effects of Cordia myxa extract on breast cancer cell line and the expression level of both caspase-3 gene and proteins. Methods: In this experimental study, the MCF-7cell line was selected for treatment with the various concentrations of Cordia myxa hydro-alcoholic extract. Cell proliferation was evaluated using MTT-method. Additionally, the impact of the Cordia myxa extract on the expression level of caspase-3 gene were examined using quantitative real-time polymerase chain reaction (qRT-PCR). Finally, the levels of caspase-3 protein have been evaluated with western bolt method. Results: Cell proliferation studies represented anticancer characteristics of dose-dependent of the hydro-alcoholic extract of Cordia myxa. Furthermore, Cordia myxa hydro-alcoholic extract led to increase expression of caspase-3. Conclusion: In overall, Cordia myxa can be a proper choice for designing anticancer drugs as a potential candidate for major investigations on breast cancer. This plant could have a possible application for cancer treatment as another anti-cancer agent, as well as drug poisoning.
Keywords: Breast cancer, Cordia myxa, Caspase-3
16 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 35814
Detection of cancer biomarkers by Microfluidic Immunohistochemistry
Sara Anajafi1 1 Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract Introduction: Immunohistochemistry (IHC) plays an important role in biomarker detection and precision oncology, determining the type and the stage of cancer. However, intratumoral heterogeneity, poor quantification potential, long process time, and lack of multiplexing capacity imposes some limitations for the clinical application of conventional IHC. Over the last decade, remarkable progress has been made in multiplexed diagnostics thanks to the advent of microfluidic approaches. Description: Chip-based IHC platforms enable multiplexing biomarker detection on small- sized specimens for personalized medicine purposes with an improved quantification and standardization. Many microfluidic IHC platforms were applied to assess tumor biomarkers comparing with conventional paraffin-embedded and cryopreserved IHC. Discussion and conclusion: This review will provide an outline of the microfluidic IHC approaches that have been applied so far to the detection of tumor biomarkers, including Programmed death-ligand 1 (PD-L1), human epidermal growth factor receptor 2 (Her2), estrogen receptor (ER), cytokeratin (CK), progesterone receptor (PR), proliferation marker Ki-67, Tumor protein p53. The microfluidic procedure was described while the qualitative and technical differences between conventional and microfluidic IHC in the assessment of the mentioned markers have been compared. Keywords: Immunohistochemistry, Microfluidic, Lab-on-chip, Cancer biomarker.
17 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 91930
Expression Analysis of Has-mir-629-5p in Cancerous Tissues from Iranian Patients with Colorectal Cancer
Mohammad Abdolvand1*, Simin Hematti2, Mohammad Hassan Emami3, Mansoor Salehi4, Farinaz Khosravian1
1. Cellular, Molecular and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran, Medical Genetics Research Center of Genome,Isfahan University of Medical Sciences, Isfahan, Iran 2. Associate professor, department of radiotherapy and oncology, faculty of medicine, isfahan university of medical sciences, isfahan, iran 3. Professor of gastroenterology and hepatology, department of internal medicine, school of medicine, al-zahra hospital, isfahan university of medical sciences 4. Member of the Board of Genetics of the Ministry of Health, Founder of Genome Medical Genetics Center
Introduction and Objectives: The incidence and mortality rate of colorectal cancer (CRC) is significant around the globe, which leads to understanding the early molecular events of CRC. During the last decades, there has been tremendous growth in the biology of microRNAs as the potential therapeutic targets in cancer. MicroRNAs are small, non- coding RNAs which regulate gene expression at the post-transcriptional level and they may function as both oncogenes and tumor suppressors. Moreover, they play a vital role in different physiologic processes in the initiation and progression of cancer. Has-miR-629- 5p in several cancers was upregulated and play critical roles in tumor progression by various cellular functions. However, the role of miR-629-5p in colorectal cancer remains unclear. Materials and Methods: In this study, we evaluated the expression of miR-629-5p in both 40 cancerous tissue samples and 40 adjacent healthy tissue samples achieved from patients with colorectal cancer. All the RNA molecules extracted from tissue samples by using trizole. cDNA was synthesized by stem-loop RT-PCR technique. The expression of miR- 629-5P evaluated by quantitative real-time RT-PCR in both case and control groups. Results: The results showed that the expression levels of miR-629-5p increased noticeably in tumor samples than normal tissues. The statistical analysis was performed via Prism software which showed (p value< 0.05). Conclusions: In overall, our findings illustrated that, miR-629-5p could be used as a promising biomarker for diagnostic and prognostic in different stages and also a potential therapeutic target in colorectal cancer. Keywords: MicroRNA, colorectal cancer, mir-629-5p, RT-PCR
18 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 61482
MicroRNA Binding Site Polymorphism in Inflammatory Genes Associated with Colorectal Cancer: Literature Review and Bioinformatics Analysis.
Maryam Zarin1, Mohsen Soosanabadi1, Sharifeh Khosravi2
1. Department of medical Genetics, Semnan University of Medical Sciences, Semnan, Iran. 2. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Abstract: Inflammation, among environmental risk factors, is one of the most important contributors to colorectal cancer (CRC) development. Chronic inflammatory conditions are involved in 15% of all epithelial tumors. In this way, studies revealed that the incidence of CRC in inflammatory bowel disease (IBD) patients is up to 60 percent higher than the general population. MicroRNAs (miRNAs), small noncoding RNA molecules, have attracted excessive attention due to their fundamental role in various aspects of cellular biology such as inflammation by binding to the 3'-untranslated regions (3'-UTR) of pro and anti- inflammatory genes. On the other hand, genome-wide association studies (GWAS) reported multiple single nucleotide polymorphisms (SNPs) in inflammatory genes correlated with CRC risk. Based on multiple previous studies, SNPs at 3'-UTR can affect miRNA recognition elements (MREs) by changing in thermodynamic features and secondary structure. This effect can be categorized, based on the amount of changes, into four groups including break, decrease, create and enhance. A lot of studies have shown the effect of these polymorphisms in cancer genes especially in inflammatory genes and CRC. In this paper, we will focus on functional variants in miRNA binding sites in inflammatory genes which can modulate the risk of CRC by both investigating previous studies regarding miRSNPs in inflammatory genes associated with CRC and recruiting in silico prediction algorithms to report putative miRSNPs in 176 inflammatory genes. In our analysis, we achieved 110 miRSNPs in 3'-UTR of 67 genes that seem good targets for future researches.
Keyword: Colorectal Cancer, Inflammation, Polymorphism, MicroRNAs
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 72105
Treatment with microRNAs in hepatocellular carcinoma
Zoya Najafi1, Mohammadreza Sharifi2, Gholamreza Javadi1 1. Department of Biology, Science and Research branch, Islamic Azad University, Tehran, Iran. 2. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Introduction: Hepatocellular carcinoma (HCC), also called malignant hepatoma, is the most usual type of liver cancer. Many studies have investigated expression signature of microRNAs (miRNA) in HCC patients, mainly patients who are treated with contractual therapy of this disease. Description: Using miRNAs as prognostic or diagnostic biomarkers by the molecular methods in different cancers has been widely used studied. The expression types of miRNAs are dysregulated in different cancers. Increasing evidence proving that miRNAs can act as an oncogene or a tumor suppressor. Treatment with miRNAs mostly depends on nucleic acid-based strategies. The target of these strategies is to restore the normal function of miRNAs. Discussion and Conclusion: Most current studies have concentrated on use of miRNAs as therapeutic method and significant progress has been made in this field which is promising. It becomes manifest that HCC is appearing as a model for transmission from contractual to new Treatment with microRNAs. Keywords: Hepatocellular carcinoma, microRNAs, Liver cancer
20 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 13945
A scientific journey in circulatory miRNAs; Promising diagnostic and prognostic biomarkers in pancreatic cancer
Mostafa Saghi1, Mehdi hassani2, Hamed Naghoosi1 1. AJA Cancer Epidemiology Research and Treatment Center (AJA-CERTC), AJA university of medical sciences, Tehran, Iran 2. Genetic Research Center, University of social welfare and Rehabilitation Sciences, Tehran, Iran
Introduction: Most Pancreatic cancer (PC) patients are asymptomatic before progressive stages. Globally, the incidence of pancreatic cancer is 5.5 per 100,000 for men and 4.0 per 100,000 for women. Despite modern therapeutic advances, PC is one of the most lethal of malignancies and correlated with a poor prognosis. Early diagnosis can significantly improve the prognosis. miRNAs contribute to important biological functions, such as cell cycle progression, cell differentiation, and apoptosis and when dysregulated can promote cells to tumor formation and metastasis. Also, there is strong support that circulating miRNAs can be used to identify efficient biomarkers for early detection of PC Description: CEA, CA-50 and CA19-9 as common biomarkers have limited specificity and sensitivity for early diagnosis and prognosis. Nowadays, circulating miRNAs have been reported to be a novel biomarker in human cancers. Attempts to assess the efficiency of circulating miRNAs solely or in compound with CA19-9 have yielded different results. In this paper, we overview many circulating miRNAs which reported as a potential biomarker for early diagnosis and prognosis in pancreatic cancer. For example, miRNA panel including miRNA-20a, miRNA-21, miRNA-24, miRNA-25, miRNA- 99a, miRNA- 185, and miRNA-191 had the overall accuracy 86.8% compared with 76% for CA19-9. Among them, miR-21 was the most frequently identified dysregulated miRNA. Therefore, this review is aimed to provide an overview of previously published studies on circulating miRNAs on the association between blood miRNAs and the PC risk. Discussion and Conclusion: When multiple miRNAs were used together, higher sensitivities and specificity can be obtained. Developing a combination of miRNA markers may be a promising approach for early detection of pancreatic cancer. Keyword: Pancreatic Cancer, miRNAs, Diagnosis, Prognosis, Biomarker
21 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 92246
The Evolving Role of Immune Checkpoint Inhibitors in Ovarian Cancer
Samin Ghaderian
Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Background: Epithelial ovarian cancer (EOC) is the leading cause of death for gynecological cancer. The standard treatment for advanced stage is the combination of optimal debulking surgery and platinum-based chemotherapy. Nevertheless, recurrence is frequent (around 70%) and prognosis is globally poor, new therapeutic agents are needed to improve survival. Immunotherapy has shown promise in other cancers such as melanoma, bladder, lung, leukemia, and breast. Immune checkpoint inhibitors for ovarian cancer ICPIs impede the ability of the tumor to activate checkpoint proteins on the surface of T cells, thereby preventing the cancer from evading immune response and allowing the immune system to generate an antitumor response. Co-stimulatory receptors that promote the activity of T cells include CD28, OX40, GITR, CD137, CD27, and HVEM. Checkpoint receptors that decrease T cell activity include PD-1, CTLA4, TIM-3, BTLA, VISTA, and LAG-3 The ligands that bind to and activate both categories of receptors are commonly found on the surface of other cells, and the ligands for checkpoint receptors tend to be found at a much higher rate on malignant cells. The PD-1 receptor (programmed cell death) suppresses T cell responses and expression of PD-L1 or PD-L2 on the surface of tumor cells and tumor-associated macrophages is therefore a major mechanism of immune evasion. PD-1 is also highly expressed on Tregs, and activation of this checkpoint receptor on Tregs increases their suppressive activity. CTLA4 is another immune checkpoint receptor that is exclusively found on T cells. The CD28 has a competitive receptor for B7 ligand, the cytotoxic T lymphocyte antigen- 4 (CTLA-4), which delivers an inhibitory signal. PD-L1/PD-1 receptor B7/CTLA-4 interactions are important immune escape mechanisms, allowing tumor progression. Immune checkpoint blockade therapeutics work by preventing these negative receptor/ligand interactions, and restoring the function of exhausted T cells. In order to prevent the activation of the immune- inhibitory pathways, several monoclonal antibodies are under development. A variety of ICPCs have gained FDA approval, yet the study of ICPIs in gynecologic cancers lags behind other disease sites. Nivolumab (Bristol-Meyers-Squibb) and pembrolizumab (Merck) target PD1.Ipilimumab (Bristol-Meyers- Squibb; targets CTLA-4, with indications in melanoma, urothelial, and lung cancers. Atezolizumab (Genentech-Roche), darvulamab (Astra-Zeneca), and avelumab (EMD- Serono) target PD-L1 and are approved for urothelial/lung cancers, bladder/lung, and for lung/Merckel cell cancers, respectively Toxicities related to ICPIs most commonly include skin (rash, pruritus), gastrointestinal tract (diarrhea), infusion-related reactions, and fatigue, which may limit the clinical effectiveness of this class of drugs. In conclusion, ovarian cancer is an
immunogenic disease and currently available data suggest a potential activity of immune
22 checkpoint inhibitors. There are still some points to be addressed.
Page Key words: immune checkpoint inhibitors, ovarian cancer
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 90148
Genetic linkage analysis of GCK and HNF1A genes in a group of families with MODY in Isfahan province
Akram Sarmadi1, Mohammad Amin Tabatabaiefar2, Fatemeh Tabatabaei3, Morteza Hashemzadeh chaleshtori4 1. MSc in Human Genetics. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, I.R. Iran 2. Medical genetics, PhD. Genetics and Molecular Biology Dept. Isfahan University of Medical Sciences. Isfahan. I.R. Iran 3. Isfahan Endocrine and Metabolism Reaserch Center. Isfahan University of Medical Sciences. Isfahan. I.R. Iran 4. Human- Molecular genetics, PhD. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, I.R. Iran
Background and aims: Diabetes mellitus is a group pf metabolic disorders resulting in increased level of blood sugar. Type 1 Diabetes (T1D), Type 2 Diabetes (T2D) and monogenic diabetes are there major groups of diabetes. Maturity-onset diabetes of the young (MODY) is a monogenic diabetes that is frequently mistaken for T1D or T2D. The aim of this study was to diagnose MODY and determine the frequency of its 2 major subgroups in Isfahan diabetic population. Methods: In this descriptive-experimental study with the aim of determining type and the frequency of mutations in GCK and HNF1A genes in 26 families with MODY from Isfahan using genetic linkage analysis method and select of 4 markers for each gene. Linkage results were confirmed by fragment analysis and then all the exons of genes were sequenced in any linked families. Results: In this study from 26 families, 4 families were linked to markers of GCK gene and 3 families were linked to HNF1A gene. After sequencing of the exons of these 2 genes in the related families, some variants were found analyzed that none of them were pathogenic mutation but some polymorphisms with increasing effects on susceptibility to diabetes were found. Conclusion: in this study despite of the fact that some families were linked to markers of these 2 gene, there were any pathogenic variant in any of the patients. So it seems that the genetic profile of this population is different from other studied populations. Keywords: Maturity Onset Diabetes of the Youngs (MODY), Genetic linkage, STR marker, GCK gene, HNF1A gene.
23 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 59442
Bioinformatics identification of phagosome-related genes and pathways with potential to be used as biomarkers of early detection of ALS
Akram Tajeddin1, Maryam Baharlooie1, Maryam Peymani2, 3, Kamran Ghaedi1, 2 1. Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran. 2. Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran. 3. Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Background and Objectives: Amyotrophic lateral sclerosis (ALS) causes progressive and irreversible degeneration of motor neurons. Until now, mutations in genes such as SOD1, C9orf72, TBK1, UBQLN2 and TARDBP have been identified in these patients, but little is known about the sporadic type of the disease, which accounts for 90% of cases. Studies have shown that Riluzole and Edaravone only work in the early stages of the disease. Therefore, early detection of this disease and timely initiation of drug administration can increase the life expectancy of patients. CSF biopsy is aggressive and has side effects, so recent studies suggest that blood may be used as an alternative. In this study, we tried to present potential biomarkers in blood by bioinformatics. Methods: Two blood and brain related datasets of ALS patients were selected from GEO database. Enrichment of biomarkers was performed on the results of microarray analysis of the blood and brain of ALS patients and selection of key genes in the disease associated with known pathways. For this step, EnrichR and DAVID databases were used and KEGG database information was used to adapt the biological pathways. Finally, the network generated by these paths was constructed with Cytoscape software and the ClueGo app. Results: Two common pathogenesis pathways in blood and brain including Phagosome and Fc gamma R-mediated phagocytosis were identified that are predicted to be related to the dysfunction of proteins in ALS pathogenesis. FCGR2B was identified as one of the key common genes in these pathways. Conclusion: The results of this bioinformatics study suggest that genes related to autophagy pathway such as FCGR2B in peripheral blood mononuclear cells could be used as potential biomarkers to increase the accuracy of early detection of ALS, and increase the chances of the drugs being effective. Keywords: Phagosome, Diagnostic biomarker, ALS, bioinformatics
24 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 67892
New insights into structural systems biology
Fariba Dehghanian Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, HezarJarib Street, Isfahan 81746-73441.
Abstract: Systems biology is a biology-based interdisciplinary field of study that focuses on the behavior and interactions of biological entities, including organisms, organs cells and macromolecules. The main goal of this field of study is using computational and mathematical tools for discovery of complex interactions within biological systems. Proteins are ubiquitous building blocks in biological systems and the dynamic networks of protein interactomes can be valuable for inferring the global features of any cellular machinery. Structural systems biology is identified as modelling protein interactions which is going to draw a molecular picture of cell. The most-critical aspects of how a biological process works results from the cooperative behavior of many types of proteins, all acting together in complexes. This places the experimental and computational aspects of structural systems biology in a critically significant position for systems biology. Most researchers have focused on two critical points of structural systems biology. In the first part, we are trying to find out the complexity of protein-protein interactions as the global features of a cell network. In the second part, we are considering proteins as a unified system of hundreds of amino acids empowers us to predict the effect of a small change on the whole system. This approach has many applications, e.g. in precision medicine. In this study, we will review all new aspects of these two fields of study in structural systems biology. We also discuss different experimental techniques and computational tools for improving our basic understanding of biological systems using structural systems biology. Keywords: Systems biology, structural systems biology, biological systems, protein interactomes, dynamic networks of protein
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 98016
Effects of five different interchain linkers on spatial structures, stability, and antigen-binding activities of the anti-CD25 scFv by bioinformatics studies
Parnian Navabi1, Mohamad Reza Ganjalikhany2, Sepideh Jafari2, Moein Dehbashi 2, Mazdak Ganjalikhani- Hakemi1*
1. Department of immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. 2. Department of Cell and Molecular Biology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.
Introduction & Objectives: Single chain fragment variable (scFv) antibodies are composed of variable heavy (VH) and variable light (VL) domains that are joined by a polypeptide linker. Several studies have focused on the effect of different length, sequence, and flexibility of linker peptides on successful construction of single-chain Fvs (scFv). The aim of this study was to find the most suitable linker peptide among of five linker peptide (GSTSGSGKPGSGEGSTKG(L1),KESGSVSSEQLAQFRSLD(L2),EGKSSGSGSESKS T (L3), (GGGGS)3 (L4), and GSAGSAAGSGEF (L5)) to construct a functional anti-CD25 single-chain fragment variable (scFv) by bioinformatics studies. Materials & Methods: Here, the 3D structures of the scFv with different linkers were predicted by Robetta server and molecular dynamics simulation was performed to compare their structures and dynamics. Then, interactions between five models of scFv and human CD25 were calculated via molecular docking. Results: Comparison of molecular dynamics simulation results presented in this study showed that the model containing (Gly4Ser)3 has more stability compared to other linkers. Additionally, based on docking results, the interaction patterns in all five models and native structure were similar and scFv-L4, scFv-L5 were better than others. Keywords: Single-chain variable fragment (scFv); Linker peptide; Molecular modeling.
26 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 66496
Different Inhibitory Activity of Abemaciclib, Hymenialdisine, and Indirubin on CDK6: A Molecular Dynamics Simulation
1 2 2 2* Amir Safi , Javad Saffari-Chaleshtori , Majid Asadi-Samani , Korosh Ashrafi- DehkordiP 1. Student Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran 2. Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
Introduction & Objectives: Cyclin-dependent kinase 6 (CDK6) is a member of cell cycle–regulating proteins that plays an important role in cancer cell growth. Some inhibitors such as Abemaciclib, Hymenialdisine, and Indirubin decrease the activity of CDK6 and cause to cell arresting. This study aims to evaluate the effects of these inhibitors on the structure of CDK6 using molecular dynamics (MD) simulation. Materials & Methods: The PDB file of CDK6 was obtained from the protein data bank server (http://www.rcsb.org). Primary simulation of CDK6 was performed by Gromacs software. Then the Molecular docking of inhibitors did on CDK6 protein in 200 stages using AutoDock v.4.2 software. The last simulation of CDK6 in presence of inhibitors had been done after docking. Results: Indirubin had the lowest value of binding energy (BE) to bind with CDK6. All three drugs significantly decreased the Root Mean-Square Deviation (RMSD). Hymenialdisine decreased the radius of gyration (Rg) strongly. It also decreased the coil structure of CDK6. Variation in the α-Helix and β-Sheet structures was significantly in the presence of Indirubin and Abemaciclib. Conclusion: All three drugs reduce the activity of CDK6. Although Hymenialdisine shows a lower tendency to bind to CDK6 than Indirubin and Abemaciclib, it decreases the availability of the active site. Also, these three inhibitors hamper protein from its proper function through altering its secondary structures. Keywords: CDK6, Abemaciclib, Hymenialdisine, Indirubin, Molecular Dynamics
27 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 89076
Bioinformatics identification of miRNAs associated to Amyotrophic Lateral Sclerosis
Maryam Safaeipour1, Maryam Baharlooie1, Maryam Peymani2, 3, Kamran Ghaedi1, 3
1. Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran 2. Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran 3. Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
Introduction: ALS is the most common disease of the upper and lower motor neurons. Given the potential of miRNAs as molecular biomarkers, the aim of this study was to identify miRNAs targeting key pathways of ALS that have a potential of early detection in the blood of ALS patients. Methods: In this study, genes related to ALS were collected from PubMed database articles. Genes with expression changes with P-value less than 0.05 in microarray data analysis in the GEO datasets on peripheral nervous tissue and blood of ALS patients were selected for the next phase. Enrichment of the biological pathways of these genes was performed using Enrich R tool. We then selected the common pathway between the two tissues, and finally, using advanced search of the MiRTarbase database from the common pathway, we obtained validated miRNAs for these pathways. Finally, tissue expression of these miRNAs was assessed using miRmine bioinformatics database. Results: After comparing the data of the nervous system and peripheral blood of ALS patients, we found a common biological pathway of oxidative phosphorylation. Finally, using the MiRTarbase database a set of hsa-miR-1-3p, hsa-miR-124-3p, hsa-miR-101-3p, and hsa-miR-155-5p, interacting directly with the gene of the specified pathway were obtained and the resulting network was analyzed and visualized with Cytoscape 3.6.1. Conclusion: Access to cerebrospinal fluid is only possible through Lumbar Puncture and is an invasive method, therefore, following an easy access alternative tissue containing the miRNAs involved, in this study peripheral blood, miRNAs similar to nerve tissue have been found and are potential to be targeted to the patient's nerve tissue to aid in the early detection of ALS. Keywords: miRNA, biomarker, Amyotrophic Lateral Sclerosis, oxidative phosphorylation
28 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 36401
LncRNAs Role in of Glioblastoma Multiform Carcinogenesis
Madineh Mazarei1, Arash Poursheikhani2 1. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran 2. Medical Genetics department, Mashhad University of Medical Sciences, Mashhad, Iran.
Introduction: Glioblastoma multiform (GBM) is of the most invasive and most frequent cancer of primary brain origin. Treatment of GBM is still an important challenge. Despite the high risk that patients face when undergoing aggressive therapies such as surgery, radiation therapy, and chemotherapy, GBM remains difficult to treat, with an average survival of 14 months. However, the exact underlying mechanisms of GBM pathogenesis are not clear yet. Thus, there is an urgent need to indicate promising potential diagnostic and prognostic biomarkers in GBM patients. Description: the RNA-seq, miR-seq, and clinical data of GBM were retrieved from the TCGA database. The “DESeq2” package in R software was used to determine the differentially expressed genes. For in silico functional analysis, GO and KEGG signalling pathways were used. Discussion and conclusion: The data indicated that 377 lncRNAs, and 220 miRNAs were differentially expressed in GBM. The GO and KEGG pathway analysis has been shown that the differentially expressed mRNAs were primarily enriched in important signalling pathways in cancer. Taken together, data showed the prominent differentially expressed mRNAs, lncRNAs, and miRNAs in GBM. Our data suggested desired lncRNAs in the tumorigenesis of patients with GBM. Keywords: glioblastoma multiform; Tumorigenesis; Long non-coding RNAs; miRNA
29 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 55720
Bioinformatics evaluation of hsa-miR-1289 signaling pathways and its associated rs10277413 function in patients with Non-small-cell lung cancer
Eman Koosehlar1, Farzaneh Ahmadi Shapoorabadi2, Hassan Mohabatkar3
1. Department of Biotechnology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran 2. Department of Biotechnology, Faculty of Biological Science and Technology, Shahid Ashrafi Esfahani University, Isfahan, Iran 3. Department of Biotechnology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran
Introduction & Objectives: Non-small-cell lung cancers (NSCLCs) are the most common lung cancers and account for more than 80% of all lung cancers. Overexpression of EGFR has been reported and implicated in the pathogenesis of many human malignancies, including NSCLC. Materials & Methods: To get more information we used NCBI, miRNASNP, miRBase, miRWALK2.0, DAVID databases and KEGG pathway as the bioinformatics tools. Results: Investigations at the rs10277413 locus indicated that T allele altered to A and G, which may effect on function of the miRNA associated with this region. Finally, hsa-miR- 1289 as one of the most important miRNAs related to EGFR and given the confirmation of the role of hsa-miR-1289 in NSCLC through bioinformatics databases, this miRNA selected for this analysis. Conclusion: The studies due to rs10277413 have shown that when the SNP locus is in the mutant A or G allele, the miRNA binds to it less strongly than the wild T allele. So when this SNP occurs, hsa-miR-1289 has less inhibitory effect on it and EGFR is transcribed more. Due to role EGFR in cell proliferation, we predicted that a person with this harmful polymorphism is more susceptible to NSCLC by increasing the expression of the EGFR. Key words: Non-small-cell lung cancer, hsa-miR-1289, NSCLC, EGFR, KEGG pathway
30 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 11405
In silico pathway-based drug repurposing for HER2-positive subtype of breast cancer using drug database mining
SeyedehMozhdeh Mirmohammadi1,2, Anvarsadat Kianmehr1,2* 1. Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran 2. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, Iran
Introduction & Objectives: Breast cancer is the most frequent cancer and also the leading cause of cancer mortality among women. It subdivides into 4 different types including HER2-positive. Here we intended to use a repurposing strategy to suggest drugs for potential application in breast cancer management. Materials & Methods: Gene expression datasets for different subtypes of breast cancer were extracted from “GEO” database. Data normalization, batch effect removal, and calculation of differential expression of genes were carried out using R software packages. The cut-off criteria used for selecting significant up-regulated genes in HER2 subtype were adjusted p-value<0.05 and log2-fold change>+3. Using Enrichr webserver, gene set enrichment was performed for significant up-regulated genes and significant up-regulated pathways were selected applying adjusted p-value<0.05 filter. The pathways highly associated with breast cancer underwent pathway analysis using KEGG mapper online tool. Critical genes in each pathway were considered as potential therapeutic targets. Drugs for other indications were repositioned for breast cancer treatment using GeneCards database which mines databases including DrugBank, International Union of Basic and Clinical Pharmacology, and The Drug Gene Interaction Database. Results: The up-regulated geneset was mainly associated with cell cycle, IL-17 signaling pathway, pyrimidine metabolism, and cytokine-cytokine receptor interaction. Afterwards, pathway analysis provided the evidence for the likely contribution of PTTG1, CDC6, DBF4, CDK1, TTK, HSP90AB1, CTPS1, TNFRSF12A, and INHBA in breast cancer progression. Finally, Fostamatinib, Alvocidib, Alsterpaullone, DB15498, Bevacizumab, Geldanamycin, and Radicicol were identified as inhibitors of candidate genes. Conclusion: Bevacizumab, an approved medication for breast cancer, included in our proposed drug list may represents a verification for the adopted drug repurposing approach. Therefore, the rest of drugs could potentially be used for breast cancer treatment after passing experimental authentications. Keywords: Drug repurposing, pathway analysis, breast cancer, HER2+, in silico
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 34991
Gene expression profile analysis during mouse tooth development
Zohreh Salimi1, Kolsoum Inanloo Rahatloo2* 1. Dentistry student, Faculty of Dentistry, Arak University of Medical Sciences, Arak, Iran 2. Assistant Professor, School of Biology, College of Science, University of Tehran, Tehran, Iran.
Background and objective: Complex molecular pathways involve in development of different tissues such as teeth. Differential gene expression patterns during teeth development generates different tooth types. Teeth development results from interactions between oral epithelium and underlying ectomesenchyme cells with neural crest origin. Teeth development are regulated by different signaling networks. In this study, we used RNA sequencing to investigate the molecular pathways underlying the development of molar teeth in mice. Materials and Methods: In current study, using data from Gene expression omnibus (GEO), we analyzed the transcriptome expression profiles of mouse teeth. A copy of the RNA-Seq raw files was downloaded from GEO and extracted reads were subsequently analyzed using specific software. Differentially expressed genes and molecular pathways during mouse teeth development were extracted. Finding: We found that Wnt5a, Slpi, Aox12b, Sprr1a and Crct1 are important differentially expressed genes during mouse molar tooth development. The important molecular pathways differentially expressed during molar development are proteasome, Wnt signaling pathway, gap junction, ERK-MAPK cascade and TNF-alpha NF-kB signaling cascade. Conclusion: Our findings indicated that proteasome pathway, Wnt signaling pathway and TNF-alpha NF-kB signaling cascade activated during early stages of molar teeth development (embryonic day14-16) and gap junction, Notch signaling and ERK-MAPK cascade activated during late stages (embryonic day16-18) of molar teeth development. Keywords: Transcriptome – Differentially Expressed Genes- Signaling Pathways
32 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 86730
Emerging roles of the Hippo signaling pathway in non-small cell lung cancer: An In Silico study
Laleh Ebrahimi Ghahnavieh1, Fariba Dehghanian*1 1. Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, HezarJarib Street, Isfahan 81746-73441, Iran *Correspondence author;
Introduction & Objectives: Non-small cell lung cancer (NSCLC) is identified as one of the most important causes of cancer-related mortality. The pathophysiology of NSCLC is complicated and different signaling pathways are associated with NSCLC tumor progression. It has been shown that dysregulations of the Hippo pathway are associated with cell overgrowth and tumorigenesis. In this study, we highlight the Hippo pathway role in NSCLC to find out dysregulated effectors and candidate drug targets for new treatment strategies. Materials & Methods: Microarray data of lung cancer tissues and normal samples were obtained from GSE10799. The screening of gene expression profile was accomplished to reach an evaluation of the data quality and extraction of differentially expressed genes (DEGs) between cancer and normal tissues using TAC software. Furthermore, a protein- protein interaction (PPI) network was constructed to determine the hub genes key modules using STRING and Cytoscape software. Results: A total of 18 DEGs out of 1,597 DEGs were recognized as Hippo pathway core components based on biological processes of KEGG. The PPI network of Hippo pathway core components was sorted and identified, which are mainly classified as cell-cell adhesion, positive regulation of vasculature development, angiogenesis, protein kinase activity and focal adhesion proteins. ITGA1, ITGA8, ITGB1, PPP1R14A, PTK2, CTGF, CDH3, CDH5, CDH6, CDH19, PLCB4, TEK, and FGFR4 were also identified as regulatory nodes of Hippo pathway. Furthermore, Expression and survival analysis were conducted in order to screen and validate key genes. TEK, LIPD, CTGF, CDH5, and ITGA8 were identified as regulatory components of the Hippo pathway in NSCLC. Conclusion: In conclusion, the present study provides new insights into the regulatory mechanisms of the Hippo pathway in NSCLC. Identification of hub genes as novel players in NSCLC may be reflected as potential new targets to improve the low survival rate in NSCLC patients. Keywords: Non-small cell lung cancer (NSCLC), Hippo pathway, Microarray, PPI
network
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 90564
Bioinformatics evaluation of hsa-miR-542-3p signaling pathways and its associated rs3088012 function in patients with chronic myeloid leukemia.
Farzaneh Ahmadi Shapoorabadi1, Eman Koosehlar2, Massoud Houshmand3, Atefeh Zamani4, Nasrin Fattahi5 1. Department of Biotechnology, Faculty of Biological Science and Technology, Shahid Ashrafi Esfahani University, Isfahan, Iran 2. Department of Biotechnology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran 3. Medical Genetic Department, National institute for Genetic Engineering and Biotechnology 4. Gene Raz Buali, Genetic and Biotechnology Academy, Isfahan, Iran 5. Gene Raz Buali, Genetic and Biotechnology Academy, Isfahan, Iran
Introduction & Objectives: Chronic Myeloid Leukemia (CML) is a slow-growing cancer of the blood-forming tissue. In chronic myeloid leukemia the bone marrow produces too many white blood cells. CML is characterized by the presence of the Philadelphia chromosome (Ph+), which contains the oncogenic BCR-ABL1 fusion gene resulting from a translocation between chromosomes 9 and 22. ABL1 is mutated in 8.82% of CML patients. Materials & Methods: To get more information we used NCBI, miRNASNP, miRBase, miRWALK2.0, DAVID databases and KEGG pathway as the bioinformatics tools. Results: Investigations at the rs3088012 locus indicated that T allele altered to C, which may effect on function of the miRNA associated with this region. Finally, hsa-miR-542-3p as one of the most important miRNAs related to ABL1 and given the confirmation of the role of hsa-miR-542-3p in CML through bioinformatics databases, this miRNA selected for this analysis. Conclusion: The studies due to rs3088012 have shown that when the SNP locus is in the mutant C allele, the miRNA binds to it less strongly than the wild T allele. So when this SNP occurs, hsa-miR-542-3p has less inhibitory effect on it and ABl1 is transcribed more. Due to the inhibitory action of MDM2 on p53, we predicted that a person with this harmful polymorphism is more susceptible to CML by increasing the expression of the ABL1. Keywords: Chronic Myeloid leukemia, hsa-miR-542-3p, CML, ABL1, KEGG pathway
34 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 65838
Comparative study of the binding affinity of natural compounds for the prevention and treatment of breast cancer using molecular docking.
Negar Hosseini *1, Marzieh Abolghasemi 1, Fatemeh Kheiri 1 1. Genetic Division, Department of Biology, Faculty of Basic Science, Shahrekord Univeristy, Shahrekord, I.R.Iran.
Introduction &Objectives: Considering the importance of induction of apoptosis in cancer, discovering an anti-cancer drugs able to induce apoptosis is one of the significant approaches in cancer treatment. Bioactive compounds such as vincristine and vinblastine (stopping the Microtubules formation by binding to the Tubulin protein) as well as Doxorubicin and Etoposide (inhibiting of Topoisomerase II) induce programmed death in breast cancer cells. The present insilico study compares the potential anticancer ability of compounds mentioned above. Materials & Methods: Firstly, the structures of Vincristine, Vinblastine, Etoposide and Doxorubicin compounds (with the access codes of DB00541, D00570, DB00773 and DB00997, respectively) were obtained from the Drugbank database. Afterwards, the pdb files of Tubuline (thr target of Vincristine and Vinblastine ligands) and Topoisomerase II (the target of Etoposide and Doxorubicine ligands) proteins with the access codes of 5J2T and 3QX3 were also prepared from Protein Data Bank database, respectively. Lastly, after considering the binding tendency of above-mentioned ligands with their targets done by AutoDockTools-1.5.6 software, the results were analysed. Results: According to the results of docking studies, Doxorubicin-topoisomerase II (- 16/09 kcal/mol) and tubiline-Vincristine (-3/26 kcal/mol) complexes have the most negative binding energy compared to Etoposide-topoisomerase II ( -12/74 kcal/mol) and tubuline-Vinblastine (-2/57 kcal/mol) complexes. Therefore, they are more likely to bind to their target proteins. Conclusion: Finally, based on the results of docking studies and the comparison of findings, it can be concluded that among the investigated compounds, Doxoruicin and Vincristine would act more effectively in inducing the pathway of cell death and treatment of breast cancer. Keywords: Breast Cancer, Apoptosis, Natural Product, molecular Docking.
35 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 65651
Thymoma, association with myasthenia gravis and molecular biomarkers: A case report
Zahra Sadri1*, Mehrdad Zeinalian1,2 1. Ala Cancer Prevention and Control Center, Isfahan, Iran 2. Department of Genetics and Molecular biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Thymoma is a malignant epithelial tumor of the thymic gland, most common in anterior mediastinum and includes eight types. Type B2 thymoma is refer to mixed epithelial cell and lymphocyte or cortical. 33 - 40% of patients with thymoma have myasthenia gravis and 15 - 21% myasthenia gravis patients have thymoma. Myasthenia gravis is a T cell- dependent autoantibody-mediated disease. We report a 44-year-old male patient with myasthenia gravis and thymoma type B2 diagnosis in thymus gland and anterior mediastinum with lung metastasis. He got different clinical and molecular diagnostic measures such as histological investigations, IHC, M.D.C.T scan, etc. CD45 (LCA) plays a major role in immune system. Disruption of the equilibrium between protein tyrosine kinase and phosphatase activity (from CD45 and others) can result in immunodeficiency, autoimmunity or malignancy. It also required for thymic selection. Lymphcytes of the patient expressed CD45 around 84% and in tumor it also has been expressed. The relationship between thymoma and thymic carcinoma is poorly understood. EMA is an effective marker to establish the epithelial nature of neoplastic cells. Staining of tumors is clearly related to the degree of tumor differentiation. EMA was reported positive in tumor IHC. Almost all of thymic carcinomas show positive staining for CD5. Although concurrent with the surgery CD5 was negative in Tumor IHC, it was reported 79% in BMA IHC two years later. The results of BMA immunophenotyping showed the expression of CD2(87%), CD3(80%), CD7(71%), CD34(1.5%), CD117 (1.3%), CD19(1.2%), CD10(1.2%), CD20(1%) on Lymphocytes. We suggest some other biomarkers test. LOH in 6q25.2-25.3 is the most common chromosomal deletion and FOXC1 gene is a potential target. HRAS, NRAS, and TP53 mutations have also been reported in some thymomas. Keywords: thymoma, biomarker, cancer
36 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 85526
Identification of relationship between athero-miRs with the ribosome biogenesis pathway in recurrent acute coronary syndrome by transcriptome analysis
Ali Zohoor1, Maryam Baharlooie2, Kamran Ghaedi2, 3 1. Department of Biology, Payame Noor University, PO BOX 19395-3697 Tehran, IRAN 2. Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, IRAN 3. Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, IRAN
Introduction & Objectives: Atherosclerosis is a chronic inflammatory disease that begins with the accumulation of fat-filled foam cells in the intimal layer of the artery. MiR-712 is known as a murine mechanosensitive miRNA regulated by disturbed flow in endothelial cells derived from an unexpected source, the primary ribosomal RNA, and targeting TIMP3 pro-atherogenic reactions. In this study, a human athero-miR with a seed region similar to this microRNA was screened for targetome and mapped to the transcriptome of patients PBMCs with recurrent acute coronary syndrome. Materials & Methods: A set of genes with 3'UTR potential to bind to the seed region of interest was obtained from TargetScanHuman7.2 databases and analyzed for pathway enrichment in KEGG repository data. Also, blood cell transcriptome of patients with recurrent acute coronary syndrome was extracted from GSE34822 dataset with downregulation by p-value <0.01 and this gene set was also analyzed for pathway enrichment in KEGG repository data. Taken, and compared with the results of the previous step. The miRNA-mRNAs interaction network was analyzed and visualized with Cytoscape3.6 software. Results: Blood transcriptome analysis of patients with recurrent acute coronary syndrome revealed that the ribosome biogenesis pathway was suppressed and genes including EBNA1BP2 and NSA2 were potential targets of athero-miRs in this pathway. Conclusion: Interestingly, some other studies confirm the role of the ribosome and ribosomal L13a protein in macrophages in susceptibility to atherosclerosis in mice. Therefore, according to the results of our study, targeting mechanosensitive athero-miRs associated with ribosome biogenesis may suggest a new therapeutic pattern in atherosclerosis recurrence. Keywords: Coronary heart disease recurrence, athero-miR, ribosome biogenesis, transcriptome analysis
37 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 91188
An Overview of Biological Effects and Clinical Application of Tumor Treating Fields in Oncology
Ahmad Reza Farmani 1*, Maedeh Mohammad Salehi 2, Jafar Ai 1, Sadegh Mohammadi 3
1- Tissue Engineering and Applied Cell Sciences Department-School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. 2- Pharmaceutical Chemistry Department, Pharmaceutical Science Branch Islamic Azad University, Tehran, Iran. 3- Department of Plastic Engineering, Faculty of Polymer Processing, Iran Polymer and Petrochemical Institute, Tehran, Iran.
Introduction: Cancer is a group of diseases including abnormal cell proliferation with the potential to spread to other parts of the body. It is one of the most important reasons for deaths and it is increased dramatically. Many treatment options for cancer exist that they are used depends on the type, location, and grade of cancer. Unfortunately, most of them are very invasive that even many of patients receiving treatment for invasive cancer die from that cancer or its treatment. Thus developing none invasive treatment for cancer is very important. Description: The biologic effects of the electrical field in different tissues are dependent on both the intensity and frequency of the stimulating electric field. Accordingly, Dr. Yorum Palti developed technology to deliver electric fields to tumor cells by applying insulated electrodes. His experiments showed that cell viability was profoundly affected at frequencies between 100 and 250 kHz and also, a narrow peak of cytotoxic effect in all cell types tested between 150 and 200 kHz, but little or no detectable effect below 50 kHz or beyond 500 kHz. Given the cytotoxic effects of these alternate electrical field in this frequency range were referred to as Tumor Treating Fields (TTFields). Discussion and conclusion: TTFields act on rapidly dividing cancer cells by disrupting mitotic spindle during metaphase/anaphase and also by producing nonuniform electric fields in cancer cells affecting intracellular organelles and macromolecules during cytokinesis and causing abnormal chromosomal segregation and multinucleation (telophase) affecting further replication of daughter cells. It can be used as a powerful none invasive treatment method for cancer treatment. Key words: Oncology, Tumor Treating Fields, none invasive, cancer.
38 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 40990
An Electrospun Mat Composed of Collagen and Polycaprolactone Loaded with Low Concentration of Silver Sulfadiazine Can Promote Wound Healing in Rats
Fereshteh Nejaddehbashi1*, Vahid Bayati1&2, Mahmoud Orazizadeh1&2 Mahmoud Hashemitabar1&2, Mohammadreza Abbaspour 3, Eskandar moghimipour1&4, 1. Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 2. Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 3. Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran. 4. Nanotechnology Research center, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Abstract: Designing a nanofibrous composite with a suitable thickness using natural and synthetic materials containing antibiotic agents with controlled release can be an appealing and critical research topic. A synthetic polymer like polycaprolactone (PCL) and a natural polymer such as collagen (COLL) possess considerable potentials in the domain of tissue regeneration. In this study, an electrospun blend of PCL and COLL type-I was loaded with low concentration of silver sulfadiazine (SSD) as an antimicrobial agent and then this characterized mat was applied in vivo. PCL/COLL membrane containing 0.1% SSD with 500 µm thickness was prepared via electrospinning. Viscosity of solutions, characterization of nanofibers, presence of SSD in nanofibers, surface hydrophilicity of nanofibers, release pattern of SSD, antimicrobial properties, as well as cellular viability using human dermal fibroblasts (HDFs) on the scaffold were also examined. A model of rat wound was then applied to assess the effects of PCL/COLL/SSD scaffold on wound healing process in vivo. This combination showed appropriate normal characteristics with desirable skin wound healing. The low concentration of SSD also revealed in vitro antibacterial effects against Staphylococcus aureus (S.aureus) and Pseudomonas aeruginosa (P.aeruginosa). Moreover, attachment and spreading of HDFs on this nanofibrous composite was reported to be in an optimal condition. As well, in vivo evaluation demonstrated that full repaired skin had been observed on day 21 in the PCL/COLL/SSD group after being wounded. The PCL/COLL/SSD mat could be considered as a suitable wound dressing of nanofibrous membranes in future in order to shorten healing time and also prevent scarring and infection during wound healing process. Keywords: Silver Sulfadiazine, Antibacterial Wound Dressing, Wound Healing, Drug
39 Release, Polycaprolactone/Collagen Electrospinning
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 81319
The differentiation of mesenchymal stem cells into photoreceptor like cells under the influence of the temporary increase of miR-96 expression
Samira Asgharzade1, Mohammad-Reza Mahmoudian-Sani2, Najmeh Fattah1 1. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran 2. Thalassemia and Hemoglobinopathy Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Abstract: It is well established that the main cause of retinal degeneration is the death of retinal photoreceptors, while effective treatment protocol is currently unavailable. Data from preclinical and clinical trials show that stem cell therapy is a useful way of treating retinal degeneration problems .On the other hand, previous works have shown that miR-183 cluster, miR-183 and miR-96, significantly affects photoreceptor maturation and maintenance in animal models. The aim of this study was to investigate effect of the temporary increase of miR-96 expression on differentiation of mesenchymal stem cells into photoreceptor like cells. Here, we show that transfection of human bone marrow-derived mesenchymal stem cells with miR-96 using the Lipofectamine induce differentiation and .progenitors genes expression consisted of CRX ،OTX2 ،PKC ،RHO and Recoverin Moreover, the up regulation expression of transcription factors CRX and RHO indicates that miRNA-96 could serve as a crucial function in differentiation of mesenchymal stem cells into photoreceptor like cells. These findings may provide a new strategy to improve usage of bone marrow stem cells as treatment for retinal dysfunction. Keywords: Human Bone Marrow-Derived Mesenchymal Stem Cell, miRNA-96, photoreceptor cell
40 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 26702
Isolation and characterization of secreted exosomes from adipose tissue- derived mesenchymal stem cells
Mohsen Sheykhhasan1, Azar Shheikholeslami1, Naser Kalhor1, Zahra Ebrahimi2, Hoda Fazaeli1∗ 1. Department of Mesenchymal Stem Cells, Academic Center for Education, Culture and Research (ACECR), Qom Branch, Iran 2. Department of Mesenchymal Stem Cells, Academic Center for Education, Culture and Research (ACECR), Qom Branch, Iran ∗ Corresponding Author
Background: Mesenchymal stem cells (MSCs) are among the most common types of adult stem cells used in therapeutic medicine having a high rate of differentiation and repair potential. It is known that stem cell derived extracellular vesicles (especially exosomes) have similar functions to their origin cells including providing cell to cell communication which can be used as an appropriate mechanism to the gene or drug delivery in therapeutic medicine. As a result, isolating and characterizing of exosomes could be promising to achieve this goal. In this study we will isolate and characterize the exosomes secreted from adipose tissue derived MSCs (AD-MSCs).
Materials and methods: After obtaining adipose samples from patients following liposuction, MSCs were isolated from the adipose tissue using an enzymatic method. Then the isolated cells were cultured in an incubator at 37°C and 5% CO2. After confirming MSCs by flow cytometry of CD90, CD44, CD34 and CD45 markers, the conditioned medium of AD-MSCs at the third passage were used to exosome isolation according to manufacturer’s protocol of exocib kit. The size and morphology of isolated exosomes were assessed using transmission electron microscopy. Additionally, the concentration of exosomes was determined by the Bradford method.
Results: After culture and passage of AD-MSCs their conditioned medium at the third passage was collected following 48 hours of culture and exosomes were extracted. The electron microscope showed that the isolated exosomes had a spherical shape with a diameter of 40-150 nm. Also the mean concentration of exosomes was 100 mg/ml.
Conclusion: Considering the important role of exosomes in therapeutic medicine the method of isolation and characterization presented in this paper can be an effective and fundamental step for future research studies in the delivery of genes or drugs for treatment purposes.
Keywords: Exosome Isolation. Characterization. Adipose-derived mesenchymal stem cell. Therapeutic medicine
41 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 76747
Graphene quantum dots (GQDs) and Fibrin Hydrogel Scaffold Can Induce Osteogenic Differentiation in Wharton’s Jelly derived Mesenchymal Stem Cells
Kaveh Khazaeel1*, Tayebeh Mohammadi2, Mohammad Khosravi3, Fatemeh Khademimoghadam 4, Fereshteh Nejaddehbashi5 1. Department of basic sciences, Faculty of veterinary medicine, Shahid chamran university of Ahvaz, Iran 2. Department of basic sciences, Faculty of veterinary medicine, Razi uvinersity, kermanshah,iran 3. Department of pathobiology, Faculty of veterinary medicine, Shahid Chamran University of Ahvaz, Iran 4. Graduated Student, Faculty of science, Shahid Chamran University of Ahvaz, Iran 5. Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Background and aim: Bone tissue engineering has a progressive role in bone regenerative medicine. Osteogenesis induction is an important process in bone regeneration. Fibrin as a bio- polymeric material and graphene quantum dot (GQD) as a induced differentiation factor have been used as a common material in bone tissue engineering researches. The current study, evaluated the effects of fibrin and GQD on the osteogenic potential of WJ- MSCs. Materials and methods: Third passage WJ-MSCs were cultured on fibrin hydrogel scaffold in DMEM supplemented with 10% FBS and antibiotic in the presence and absence of 10 μg/ml GQD. The culture dishes were incubated 14 days under common cell culture conditions. Their medium changed every 3 days. At the end of the period, in vitro osteogenic potential of cells was determined by 2 common osteogenic marker tests included alkaline phosphatase (ALP) activity and alizarin red staining. Results: Mineralization was occurred within the two - 3D medium groups diagnosed as red colored regions, while, the number of red colored region in GQD group was higher than the control group. Mean ± SEM of ALP enzyme was 207.23± 18.76 and 178.74 ± 2.31 in GQD and control group, respectively however their difference was not significant (P<0.05). Conclusion: In absence of osteogenic differentiating, fibrin and GQD could induce osteogenic differentiation in WJ-MSCs. These elements can enhance osteogenic differentiation potential of MSCs. Keywords: Fibrin, GQD, Scaffold, WJ-MSC, Osteogenic, ALP.
42 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 82182
Differentially expressed genes and molecular pathways in Intellectual disability patients with mutation in Polr3b gene
Mostafa saghi1, Kolsoum InanlooRahatloo2, Afagh alavi1, Kimia Kahrizi1, Hossein najmabadi1
1 Genetic Research Center, University of social welfare and Rehabilitation Sciences, Tehran, Iran 2 School of Biology, College of Science, University of Tehran, Iran
Introduction: Intellectual disability (ID) is a clinically important disease and a most prevalent neurodevelopmental disorder. The etiology and pathogenesis of ID are poorly recognized. Polr3b gene encoding the second largest subunit of RNA polymerase III and diseases associated with mutation in this gene are Hypomyelinating leukodystrophy-8 With or Without Oligodontia And/Or Hypogonadotropic Hypogonadism and Pol III-Related Leukodystrophies. Method: To explore how genetic variants alter cell expression in ID patients, RNA sequencing on blood samples was performed. Exome sequencing revealed a homozygous missense mutation in the Polr3b gene in one ID family and to obtain insights into the biological pathways influenced by Polr3b mutation, we applied our RNA-Seq data to several gene ontology programs such as BioCarta, WikiPathways, KEGG, NCI-Nature and panther Result: Overall, 509 differentially expressed genes (p-value <0.05 and fold change> 1.5) were identified between POLR3B mutant patients and controls, of these 509 genes, 225 genes downregulated and 284 genes upregulated in ID patients. in addition to involving immune and translation systems, the most significantly dysregulated pathways were " Nonsense Mediated Decay", " Cell adhesion molecules (CAMs) ", "NOD-like receptor signaling pathway" and some metabolism pathways such as "Selenocysteine synthesis", "Arylamine" and "Sulfur". Conclusion: The current study highlights the importance of NMD and CAM molecular pathways in intellectual disability and highlights the importance of POLR3B in neuronal functions. Keyword: Intellectual Disability, POLR3B, RNA-Seq
43 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 50537
Amniotic Membrane Stem Cells (AMSc): new perspectives for cancer cell therapies
Sharareh Salmanizadeh1,2, Farinaz Khosravian1,2, Mansour Salehi1,2,3 1. Cellular, Molecular and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran 2. Medical Genetics Research Center of Genome Isfahan, University of Medical Sciences, Isfahan, Iran 3. Department of Genetics and Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran
Introduction: Stem cells are undifferentiated biological cells that have the ability to differentiate into any specialized cells capable of cell division through mitosis. AMSc are advantageous cells for such therapeutic schemes and can be more preferencable than other stem cells for characteristics. Such as, immunologically privileged (no reaction even between completely unrelated donors and recipients), no ethical, many more stem cells, a sort of hybrid between embryonic and adult stem cells in terms of their plasticity and differentiation abilities, high levels of hyaluronic acid, and potential nature anti-microbial characteristic. Description: Recent studies have elucidated that cell-based therapies are promising for cancer treatments. AMSc have immunosuppressive, antitumor, and tumor-tropism properties. Furthermore, reported that hyperthermia-treated MSCs (heat treated MSCs) exerted antitumor effects on human carcinoma. An increase in the Bax expression in cancer cells confirms the activation of apoptosis by increase in P21 and P53 gene expressions by AMSc Maintains its telomere length and natural tendency. AMSc have a chromosomal stability up to 250 doubling time and high proliferation rather than other stem cells Tumor tropism cheracteristic of AMSc can results in used as vehicles for suicide gene transfer to tumors for the process called gene-directed enzyme/prodrug combination (GEPC) therapy. In these strategies, amniotic-derived stem cells effectively target the tumor and suppressed the tumor growth by expressing various cytotoxic cytokines. Discusion and Conclusion:AMSC have advantages when introduced to other bodies because they are non-tumorigenic and cause low immunogenicity and anti-inflammation. Not only are amnionic membrane stem cells applicable in regenerative medicine, they have anticancer capacity. In non-engineered stem cells transplantation strategies, amnion- derived stem cells effectively target the tumor and suppressed the tumor growth by Owing to their own advantageous properties, amnion-derived stem cells are emerging as a new candidate in anticancer therapy.
44 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 78259
The effects of selenium and probiotics co-supplementation on glycemic status and PPAR-γ gene expression in patients with gestational diabetes mellitus: a randomized, double-blind, placebo-controlled trial
Elaheh Amirani1, Mohsen Taghizadeh1, Željko Reiner2, Zohreh Tabassi3, Esmat Aghadavood1, Ida Panahandeh3, Fatemeh Naderi3, Maryam Tajabadi-Ebrahimi4, Zatollah Asemi1* 1. Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, I.R. Iran 2. Department of Internal Medicine, University Hospital Centre Zagreb, School of Medicine, University of Zagreb, Zagreb, Croatia 3. Department of Gynecology and Obstetrics, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran 4. Faculty member of Science department, science faculty, Islamic Azad University, Tehran Central branch, Tehran, Iran
Aim: The aim of this study was to determine the effects of selenium and probiotic co- supplementation on glycemic status and PPAR-γ gene expression in patients with gestational diabetes mellitus (GDM). Methods: This randomized, double-blind, placebo-controlled clinical trial was performed in 51 patients with GDM. Participants were randomly divided into two groups to take either probiotic containing Lactobacillus acidophilus, Bifidobacterium bifidum, and Bifidobacterium longum (2×109 CFU/day each) plus 200 µg/day selenium (n=26) or placebo (n=25) for 6 weeks. Results: Selenium and probiotic significantly decreased fasting plasma glucose (β -3.96 mg/dL; 95% CI, -6.54, -1.37; P=0.003), insulin concentrations (β -1.24 µIU/mL; 95% CI, -2.03, -0.46; P=0.002) and insulin resistance (β -0.38; 95% CI, -0.60, -0.17; P=0.001), and significantly increased insulin sensitivity (β 0.008; 95% CI, 0.003, 0.01; P=0.003) when compared with placebo. This combination also upregulated peroxisome proliferator- activated receptor gamma (PPAR-γ gene expression) (P=0.01) expression levels. Conclusion: Selenium and probiotic co-supplementation during 6 weeks to patients with GDM had beneficial effects on metabolic status and gene expression related to insulin metaboliam. Keywords: Selenium, probiotics, gestational diabetes mellitus, glycemic status, PPAR-γ.
45 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 75102
Stem cells as effective carriers for viral therapy in prostate cancer
Mohsen Rezaei Aghdam1, Ali Shaker Khatibi1, Asiyeh Jebelli2 1. Biotechnology student, Department of Biological Science, Faculty of Basic Science, Higher Education Institute of Rab-Rashid, Tabriz, Iran 2. Assistant professor, Department of Biological Science, Faculty of Basic Science, Higher Education Institute of Rab-Rashid, Tabriz, Iran
Introduction: The prostate cancer is the most common disease among men in Western industrialized countries. One effective remedy for malignant tumors of prostate is using of viruses. This treatment involves the use of genetically engineered viruses or natural viruses directed toward cancer cells to cause targeted destruction of tumor cells. The most important advantage of using these viruses is their ability to distinguish between natural and cancerous tissues. However, there are important challenges in targeting tumors with viruses, foremost of which is the disposal of the host body's immune system during the first week of therapy. Moreover, the barriers inside the tumor, such as hypoxia, different portions of the connective tissue, and the growth of tumors develop complications that reduce the therapeutic ability of these viruses. Description: New studies show that the use of stem cells as carrier for the virus therapy makes them to protect against the host's immune system as well as prevent them from recruiting them by non-specific tissues. The stem cells with a diversity in their structure show the greatest effect compared to other carrier systems to use in viral therapy. Most positive therapeutic results have been reported using mesenchymal stem cells, neural stem cells and stem cells derived from fat. Due to the inherent tendency of stem cells to become tumor cells, consideration should be given to toxicity-induced viral therapy in these cells. Besides, the viral therapeutic dose and induction system should be carefully monitored to be effective in remote tumor sites. Discussion and conclusion: The use of autologe stem cells produced by the patient has more effective therapeutic results than the allogeneic stem cells that may be passed after implantation. With recent advances, it is hoped that in the near future, scientists will be able to treat metastatic patients at the clinical level.
46 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 30408
The effect of different long non-coding RNAs on MDR1 drug resistance gene in cancer
Nayereh Abdali1*, Tahereh Abdali2 1. * MSc Student of Medical Biotechnology, Faculty of Modern Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran, E mail: [email protected] 2. BSc. Public Biology, Payame Noor University of Isfahan, Isfahan, Iran
Introduction: cancer is one of the main causes of death all over the world. There are various aspects to the mechanism of resistance to chemotherapy, one of which is multidrug resistance in cancer. Multidrug resistance proteins (MRPs), known as ABC transporter proteins, responsible for the efflux of anti-cancer drugs through the cell membrane by ATP hydrolysis. Emerging evidence showed that long non-coding RNAs (LncRNAs) took a section in the regulation of MDR1 expression. Long non-coding RNAs (lncRNAs) widely exist in human various tissues, they larger than 200 nt and lncRNAs could form complex with corresponding target genes, promote or inhibit the degradation and translation of target genes and have been shown to play crucial roles in drug resistance, small part of functional lncRNAs have been well characterized with diverse regulatory roles in cellular processes, such as cell proliferation and migration. Description: This is a review study Collected by reviewing articles related to LncRNA and its effect on drug resistance with the Keywords "MDR1" OR "Multidrug Resistance" and "LncRNA" OR "Long noncoding RNA" from 2015 onwards, from Google Scholar and Pubmed it has been compiled and written. 25 articles were collected, that Of these 9 articles were excluded because of lack of subject relevance And only 16 studies were used. The inclusion criteria were all articles that examined the effect of LncRNA on MDR1. Discussion and conclusion Inclusion: Different studies have shown that LncRNAs such as DANCR, HOTAIR, PVT1, H19, SNHG5, FENDRR, tsRMST, MALAT1, BC032585, LINC00968, UCA1, Nrf2, and TUG1 can affect the MDR1 drug resistance gene. Therefore, understanding the effect of LncRNAs on the MDR1 resistance gene could be an effective step in decreasing drug resistance in cancer patients. Keywords: LncRNA, MDR1, Cancer
47 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 37358
L- Carnitine and fibrin encapsulation effects on restoration of estrous cycle and ovarian function after autotransplantation in mice
Fatemeh Shahi Sadrabadi Department of Biology, Payame Noor University, Tehran, Iran
Abstract: Acceleration of revival of ovarian function and maintaining of follicular reserve is necessary after grafting of ovarian tissue. The objective of this study was to evaluate the L-Carnitine (LC) effect and fibrin scaffold on restoration of estrous cycle and serum follicle stimulating hormone (FSH). NMRI mice were divided into six groups: Control (non- grafted), T (autografted), LC (autografted + LC), F (autografted + fibrin), LC+ F (autografted + LC + fibrin), Saline (autografted + saline). 6-weeks-old mice were bilaterally ovariectomized in the diestrus stage; then, left ovarian tissue were encapsulated in fibrin and transferred into back muscle. LC (200 mg/kg) was injected intraperitoneally one day before surgical operation and repeated until one week after grafting. Daily vaginal monitoring was performed until re-initiation of first full estrous cycle. Thereafter, follicular preservation, serum follicle stimulating hormone (FSH) levels and apoptosis incidence were assessed. Restoration of first full estrous cycle and apoptotic index in all transplanted groups were approximately similar and. however, the total numbers of follicles in transplanted groups were lower than control group. Moreover, the level of FSH in all transplanted groups was reduced significantly compared to the ovariectomized mice. In conclusion, our study, demonstrate that LC and fibrin encapsulation did not show any negative effect on transplants, and it seems that usage of scaffold in combination with a growth factor could improve the grafting results and further studies are needed in this area.
48 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 86948
PedRo: Pedigree drawing software in Persian language to facilitate the process of documenting patient’s information in medical centers with the ability to export (.pro) format
Akram Sarmadi1, Mehdi Honarmand2
1. Genetics and Molecular Biology Dept. Isfahan University of Medical Sciences. Isfahan. I.R. Iran 2. Master Sciense of Computer Engineering, Islamic Azad University, Najafabad Branch ,Isfahan, Iran
Introduction of a designed software: The pedigree drawing software are so important in genetic and medical laboratory for documenting the patients’ information. But unfortunately there are limitations in the available software in this area. Some examples of these limitations are: some tools only can run in special devises with special platforms (Windows, Linux, or Mac os) and also only in 32 or 64 bit Windows, some tools don’t have the ability to save the documantation as a recoverable file, and if the present procedure complete, it is not possible to make future change on it, some tools are not easy to use, especially for simple technicians in the medical clinics. Here, in Iran we need a tool with Persian language to be user Fridley for all the users. Furthermore, I had so much problems when I was performing my MSc thesis with the available pedigree drawing tools: some of them did not work on my laptop computer, some of them did not be recoverable and for any little changes I should to draw all the pedigree from the first. So I decided to designed a tool for the other students and also easy use in clinics. Some features of my designed PedRo tool are: Simple and intuitive graphical interface for pedigree drawing, follows conventions (symbols that used in genetic pedigree), Multiplatform (Windows, Mac OS, Linux), It can Exports file in several formats: PED, PRO, BOADICEA, JSON, It is in Persian language for easier use of all users. In the first scene of the software, with a tool bar is above of the screen, you can choose the template icon. There are some common templates and you can choose one of them to continue the pedigree drawing procedure. Then when you draw your complete pedigree you can click on each individual to change the sex, add spouse, add parent, add children, add sibling and others. Also by clicking on each individual icon, you can insert the personal and clinical features of them in their documents. Here you can select the sex, affected or healthy status, date of birth and so on. In tool bar we have an automatic layout icon that by clicking on it the layout of the pedigree will change automatically and you can decide which one is bather and more simple to understand. By clicking on renumbering icon, all the individuals will be numbered by the inserted order. This numbering is useful for finding the parsons and also is necessary for the .pro extraction file. In export icon, we can export our pedigree in several different formats. The most usable format is .pro that is not
available in every tools. Because this format is requiring to importing file in other genetic tools for example easylinkage and haplotypepainter. This format has 7 columns with specific character for 49 them. Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 67756
Static magnetic field improves post vitrification quality of mouse GV oocytes
Sara Soleimani1,2, Farzaneh Baniasadi2,3, Vida Kazemein Jasemi2, Samira Hajiaghalou2, Mohsen Gharanfoli2*, Rouhollah Fathi2* 1. Department of Cell and Molecular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and culture, Tehran, Iran 2. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran 3. Faculty of science, Physics Department, Shahid Beheshti University
Introduction and Objectives: Preservation of women fertility is a critical issue in assisted reproductive technology (ART). Nowadays, vitrification become an applicable method for banking of oocytes. Ice crystal formation is one of the main issues during vitrification and warming procedure which lead to cell cryo-injuries. Different studies showed that static magnetic field (SMF) could improve vitrification in owing its cryoprotective properties. The aim of this study was to evaluate the effect of SMF on oocyte maturation. Material and Methods: GV oocytes from 6-8 weeks NMRI mice were used in this study. Oocytes were vitrified by 2 steps vitrification, briefly, GVs were exposed to equilibration medium (7.5% EG plus 7.5% DMSO (v/v)) for 5 minutes and then washed in vitrification solution (15% DMSO, 15% EG, 0.5M/L sucrose) for 1 minute. Afterward, they were quickly loaded on cryotop and plunged into liquid nitrogen. For warming, the samples were exposed to 1, 0.5 and 0.25M/L sucrose of warming solutions for 1, 3 and 3 minutes respectively. Neodymium ring magnets with (20±2mT, 40±2mT, 60±5mT and 80±5mT) also were applied along with vitrification process in other groups. Trypan blue and JC 1 staining were used to assess viability and mitochondrial membrane potential. Results: However, the mitochondrial activity decreased significantly in vitrified and vitrified plus 80mT groups (p<0.05) in comparison to control group, but it increased in SMFs 20mT, 40mT and 60mT. There were no significant differences in viability rates between experimental groups. Although the maturation rate was not meaningfully altered in vitrified (71.6±1.7), vitrified plus 20mT (80.8±5.4), 40mT (71.4±2.22) and 60mT (78.2±5.5) groups comparing to control group (72.6±3.1), but it reduced significantly (p<0.05) in 80mT (56.3±2.4) as compared to vitrified plus 20mT group. Conclusion: It seems that vitrification along with SMF could improve vitrified oocytes quality. Keywords: In-vitro maturation, Mitochondrial membrane potential, Static magnetic field, Vitrification.
50 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 16118
Effect of oral B complex vitamin on sperm parameters and chromatin integrity in animal models of varicocele
Parisa Mohammadi1,2,3, Nasrin Bagheri1, Nayereh Torkiyan1, Mahnaz Fadaee1, Elham Naghshineh1,4 1. Majesty of Maryam Infertility Center, Martyr Beheshti Hospital, Isfahan University of Medical Sciences, Isfahan, Iran; 2. Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran; 3. Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran; 4. Department of Obstetrics and Gynecology, Infertility Center, Beheshti University Hospital, Isfahan University of Medical Sciences, Isfahan, Iran
Objectives: Varicocele is abnormal dilation and torsion in the pampiniform plexus veins of the spermatic cord. In the past decade some clinical tests approved positive effects of oral antioxidant on oxidative stress in sperm. Vitamin B is indispensable for DNA replication and synthesis, and also metabolism of amino acid and fatty acid. It acts as a coenzyme for methionine synthase enzyme in one carbone cycle that synthesises methionine from homocysteine and results in the methylation of the main functional macromolecules/molecules such as DNA, RNA, lipids, proteins, and amino acids. Homocysteine directly association with increased oxidative stress. Many studies have shown that varicocele associated with increased levels of oxidative stress on sperm and deacrease semen antioxidant capacity. Materials and methods: Male rats (n = 40) were divided into control (n = 10), sham (n = 10) and varicocele induction (n = 20) groups. From the 20 remaining rats in varicocele group, 10 rats received Vit B complex (6, 9.6, 30.4, 9.6 and 0.006 mg/kg for B6, B2, B9, B1 and B12 respectively), 10 rats only received water for 2 months. all animals were killed and both testicles were removed, and sperm were collected from the left epididymis tail. sperm parameters, DNA damage, lipid peroxidation and residual histone were adversely effected in the all groups. Result: we demonstrated that sperm parameters such as concentration, percentage of sperm motility and abnormal morphology was significantly lower in left testis of antioxidant supplements treated with B vitamin group compared to the other groups (p<0.05). While, percentage of DNA damage, histone replacement and lipid peroxidation level was significantly decreased in left testis of treatment group compared to other groups (p<0.05). Conclusion: These results showed that Vit B antioxidant was more efficient in improvement of sperm parameters, chromatin integrity and lipid peroxidation in varicocelized rats compared with others groups. Keyword: Varicocele, sperm chromatin, oral B complex vitamin, sperm parameters, lipid peroxidation
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 22057
Study of aneuploidies using karyotype, FISH and QF-PCR methods in amniotic fluid samples of 1500 pregnant women in Isfahan, Iran
Amin Izaditabar1, 2, Mansoor Salehi1, 2, 3, Elaheh Zarean3, Farinaz Farahbod3, Farinaz Tabibi1, 2, Nafiseh Ghorbani1, 2, Samaneh Ghadami1, 2, Elnaz Purabutaleb1, 2 1. Cellular, molecular and Genetics Research center, Isfahan, University of Medical Sciences. Isfahan, Iran 2. Medical Genetics Research Center of Genome, Isfahan University of Medical Science, Isfahan, Iran 3. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Introduction and Objectives: Down syndrome is the most frequent chromosome abnormality in newborns and its prevalence is about 1 in 800 newborn babies. Cytogenetic prenatal diagnosis tests can detect aneuploidies and other chromosomal rearrangements in the fetus. We did this research to investigate of incidence of Down syndrome and aneuploidies as well as translocations and other chromosomal abnormalities and evaluate of fast tests such as FISH and QF-PCR in compare with constitutional karyotype. Material and Methods: Amniotic fluid and CVS samples were collected by respective specialist, then processed and cultured with standard procedure. After slide preparations, slides stained using G-banding method and separately analyzed for each sample, and results were reported. With request of the specialist or the patient for whom that the age of the fetus was above 17 weeks, fast test was performed on the collected sample and if the case were abnormal, result was confirmed with two techniques. Results: We performed karyotype on 1500 amniotic fluid samples and also FISH technique were applied on 432 of them. Down syndrome was observed in 24 cases which 10 of them were detected using FISH. Inversion of chromosome 9 was the second frequent rearrangement in the samples and there were two cases with trisomy 13. Among these cases 5 balance translocation and one case with triploid chromosomes were reported. Conclusion: A chromosomal abnormality in the fetus depends on the maternal age and family history. 3.6 percent of the referred samples had chromosome abnormalities in which 2 percent may leads to abnormal child with moderate to severe clinical manifestations. Because of low sensitivity and specificity of screening tests, more specific tests such as karyotype and FISH can be helpful to detect of aneuploidies and also other chromosomal changes in the fetus. Keywords: Down syndrome, aneuploidy, karyotype, amniotic fluid, chromosome abnormality
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 77517
Ethical Issues in Stem Cell Research and Treatment
1Abed, Atena, 2Yaghoobi, Hajar* 1. Department of Molecular Medicine, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran 2. Department of Molecular Medicine, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran
Introduction: With the advancement of technology, significant success has been made in the field of stem cells. Due to two properties of stem cells, unlimited proliferation and conversion to different types of body cells, can be used in repairing damaged tissues and perhaps for making human organs in the future. The most important use of stem cells is cell therapy. Due to widespread use of stem cells, there are many ethical challenges to these cells in the fields of religion, ethics and politics. Ethical and religious controversial questions arise when human embryos and tissues are used to advance research and treatment. There are two main tasks in using human embryonic stem cells: Respect for human life and reduction of suffering in man. Embryo production, stem cell extraction, and subsequent destruction only to further research and therapeutic approaches have prompted various countries to adopt laws and regulations on the use of embryonic stem cells. Description: For editing this article, mainly researches were done in Pubmed and Google scholar with such topics as Ethical issue of stem cell, SCNT, IVF and so on. Most of the searched articles were from the last ten years. Discussion and Conclusion: In the present article, while referring to basic concepts and applications, mainly the ethical issues related to the use of stem cells in research and the legal status of this case in different countries are reviewed. While Iran is one of the first Muslim countries to produce embryonic stem cells in stem cell research and its therapeutic application, its activities are based on established guidelines. While there is a need to systematic laws to be adopted by parliament in this country. Keywords: Stem Cells, Cell Therapy, Ethical Issues.
53 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 60778
A novel dual thermo‑ and pH‑responsive silver nanocomposite hydrogel as a drug delivery system
Nastaran Dianatnejad 1*, Amir Reza Moghadam2 1. Department of chemistry, University of Guilan, Rasht, iran 2. Department of paramedics, University of Danesh Alborz, Qazvin, iran *corresponding author: [email protected]
Abstract: Abstract The purpose of this study was to develop a novel dual thermo- and pH-responsive silver nanocomposite hydrogel (SNH) for drug release applications. This smart SNH was prepared in a facile one-pot method by in situ reduction of silver ions poly(acrylacid) grafted onto salep polysaccharide. The SNH was characterized by transmission electron microscopy (TEM), scanning electron microscopy with energy-dispersive X-ray analysis (SEM), UV– Vis spectroscopy, and cyclic voltammetry. The dependence of swelling properties of the prepared SNH on the reaction variables (such as monomer, Ag NO3, and cross-linker concentrations), temperature, pH, and salt was investigated. The potential of obtained SNH was examined for the Doxorubicin release from prepared hydrogel under different temperatures and pHs. The evaluation of release mechanism and determination of diffusion coefficients were also studied. In addition, SNH showed good antibacterial potentials. The results of this study provide valuable information regarding the development of dual stimuli-sensitive SNH for biomedical applications. Keywords: Ag nanoparticle · Nanocomposite hydrogels · Drug delivery · Swelling
54 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 16979
PIWI-interacting RNAs as novel biomarkers for diagnosis and treatment of breast cancer
Parisa Maleki Dana1, Mohammad Ali Mansournia2, Seyyed Mehdi Mirhashemi3*
1. Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, I.R. Iran 2. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 3. Metabolic Diseases Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran Introduction: Breast cancer is one of the most leading cause of cancer-related deaths among women worldwide. Currently, there are several therapeutic and diagnostic methods for detecting and treating breast cancer patients. However, we are still facing some limitations in the fields of diagnosis and treatment. Therefore, studying the molecular mechanisms involved in breast cancer could lead to providing better care for patients suffering from this disease. Recently, non-coding RNAs have attracted a lot of attention in cancer researches due to their considerable roles in the development and progression of cancers as well as drug resistance. PIWI-interacting RNAs (piRNAs) which are a subclass of non-coding RNAs have been shown to have remarkable effects in breast cancer. Description: In this review, we provide a comprehensive overview providing studies concerned with the roles of piRNAs in breast cancer. We discuss how piRNAs can be involved in the development, progression, invasiveness, and metastasis of this disease. Moreover, findings of piRNAs which serve as diagnostic and prognostic biomarkers are summarized in this paper. Conclusion: Findings demonstrate that the expressions of some piRNAs are different in breast cancer compared to healthy breast tissue. While some of them are overexpressed, some other ones are reported to be downregulated or completely absent. Multiple piRNAs are associated with proliferation, invasion, and lymph node positivity of breast cancer. PiRNs can cause inhibitory effects on the metastasis of breast cancer. Moreover, several piRNAs alter the progression of the cell cycle. It is suggested that piRNAs act as tumor suppressors. in addition, they serve as a breast tumor marker. Studies indicated that the impacts of chemotherapeutic drugs on breast tumor can be enhanced by synergistic effects of piRNAs. Altogether, we believe that piRNAs are potential biomarkers which can be used in both diagnosis and treatment of breast cancer.
55 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 81675
Circulating Tumor Cells in the context of Personalized Medical: Diagnosis and Treatment of Cancer
1,* 1 2 Ali Shaker Khatibi , Mohsen Rezaei aghdamP ,P Asiyeh JebelliP
1. Biotechnology Student, Department of Biological Sciences, Faculty of Basic Sciences, Higher Education Institute of Rabe Rashid, Tabriz, Iran 2. Assistant Professor, Department of Biological Sciences, Faculty of Basic Sciences, Higher Education Institute of Rabe Rashid, Tabriz, Iran *Corresponding author : [email protected]
Introduction: Cancer is the result of uncontrolled growth of cells and the creation of tumors. Cancer cells can metastasize and move from one place to another place through the bloodstream or the lymphatic system. Although a significant proportion of patients diagnosed with cancer are cured by surgery or adjuvant systemic treatment, many patients still die due to the development of metastatic disease. Circulating tumor cells (CTCs) are believed to be responsible for the growth of metastatic disease. Over the past few years, the study of CTCs for understanding metastasis as well as developing diagnoses associated with predicting patient response to targeted anticancer therapies has opened new horizons for cancer diagnosis and treatment. Description: CTCs are cancer cells that have escaped from a primary tumor or metastatic site. Some of them can survive in the bloodstream, migrate to the interstitial space, and eventually lead to distant tumor formation in a new microenvironment. There are several challenges related to the detection of CTCs: (1) CTCs are rare cells in the background of large numbers of blood cells, (2) CTCs need to be diagnosed prior to the enrichment stage. Diagnosis of CTCs is often performed using immunocytological or molecular technologies for specific markers. Because CTCs are cytokeratin positive, they can be detected using antibodies against cytokeratin. These CD45-negative cells are detectable from leukocytes present in the bloodstream. In addition, functional assays such as cell culture or xenotransplantation are also used in mouse models. Discussion and conclusion: These technologies can detect diverse subtypes of CTCs with different sensitivity and purity. CTCs have great potential for usage in personalized medicine to choose effective treatments for each person based on the various types of these cells and their distinct function in different individuals.
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 50220
DNA Methylation as Diagnostic and Prognostic Biomarkers in Colorectal Cancer; Focus on Blood Strategies
Somaye ZeinalZadeh1, Behzad Narimani Eslami1, Sajad Akbarnejad1
1. Student Research Committee, Factually of Medicine, AJA University of Medical Sciences, Tehran, Iran Introduction: Colorectal cancer (CRC) is among the three most prevalent cancers. Statistics in 2018 indicate that CRC accounted for about 861000 deaths every year. Recent studies have demonstrated that colorectal oncogenesis is a multistep event resulting from the accumulation of a variety of genetic and epigenetic changes in colon epithelial cells, which can be reflected by epigenetic alterations in blood. Due to the growing prevalence of colorectal cancer (CRC), new screening and early detection methods are demanded. Among novel biomarkers, DNA methylation emerged as high potential diagnosis/screening molecular marker. In this review, we focus on DNA methylation changes as potential blood-based biomarkers for diagnosis and prognosis of CRC. Description: Several studies identified new methylation biomarkers in blood including APC, NEUROG1, SFRP2, CDKN2A/P16, hMLH1, NGFR, ALX4, RUNX3, TPEF/HPP1, TWIST1, TAC1, IGFBP3, EYA4, SST and TMEFF2. Some of them have high sensitivity and specificity until 85% and 100%, respectively. The most studied biomarker in CRC is SEPT9, which is the FDA-approved methylation-based biomarker in blood for CRC. In addition to serum and plasma, peripheral blood mononuclear cells (PBMCs) provide an intact source of biomarkers for malignancies. Studies introduced that examining promoter methylation of TFPI2 and NDRG4 genes in peripheral blood mononuclear cells (PBMCs) supply high enough sensitivity and specificity to be nominated as novel noninvasive CRC screening. Discussion and conclusion: As we extensively reviewed DNA methylation biomarkers in CRC detection. Our review shown that these types of biomarkers in blood stream are very promising for diagnosis and prognosis of CRC. In this way next research must work on specific genes that mentioned, with high sample size and facilitate methods for detection of these biomarkers. Keyword: Methylation, Biomarker, Colorectal Cancer, Diagnosis, Prognosis
57 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 50065
Molecular docking evaluation of some analogues of biarylbenzamides as HDAC1 inhibitors for cancer treatment
Rahman Abdizadeh1, Farzin Hadizadeh2, Tooba Abdizadeh3* 1. Department of Medical Parasitology and Mycology, Faculty ofMedicine, Shahrekord University of Medical Sciences, Shahrekord, Iran 2. Biotechnology Research Center, Pharmaceutical TechnologyInstitute, Mashhad University of Medical Sciences, Mashhad, Iran 3. Clinical Biochemistry Research Center, Basic Health SciencesInstitute, Sharekord University of Medical Sciences, Shahrekord, Iran; [email protected]
Introduction & Objectives: Histone deacetylases (HDACs) are attractive therapeutic targets for the treatment of cancer and other diseases. Of the four classes of HDAC found in mammals, HDAC1 has a key role in cancer and overexpressed in different cancers also, the design of HDAC1 inhibitors is an ongoing topic in drug design. Material and Methods: The molecular docking process was performed using Molecular Operation Environment (MOE) software to predict mode of interaction between the best possible biological conformations of compounds in the active site of HDAC1 enzyme. The 2D structures of compounds were prepared by Chem Draw ultra 8.0 software and converted into 3D format by Hyper Chem7 using AM1 semi-empirical method. The compounds were docked into active site of HDAC1 (PDB ID: 4BKX) by MOE software. The best pose of compounds with the higher score was selected for ligand-target interaction analysis by LigX module in MOE software. Results: The docking results showed a high docking score (-15.75 kcal/mol) for the most active compound of biarylbenzamide in comparison to that of the least active compound (- 12.45 kcal/mol) and also, HDAC1 enzyme is a zinc-dependent enzyme and zinc atom was penta-coordinated with His178, Asp176and Asp264 as well as the carbonyl and NH2 groups of the compounds. Conclusion: The docking studies showed interaction mode of biarylbenzamide derivatives with HDAC1 including zinc ion coordination, strong hydrophobic interactions and formation of hydrogen bond with benzamides. The amido and amine groups of benzamide part as scaffold and the bulk groups as a hydrophobic part were key factors to improve inhibitory activity of HDAC1 and design more potent HDAC1 inhibitors for the therapeutic of cancer. Keywords: Histone deacetylase 1; BiarylBenzamide, Molecular Docking, HDAC1 inhibitors
58 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 48127
Co-delivery of metformin and Silibinin with biocompatible PLGA–PEG copolymer nanoparticles for the treatment of non-small lung cancer
Fereshteh Mogheri1, Mehdi Dadashpour2, Akram Firouzi- amandi3, Nosratollah Zarghami4* 1- Department of Biochemistry, Institute of Higher Education Rab-Rashid, Tabriz, Iran 2- Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran 3- Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran 4- Department of Clinical Biochemistry and Laboratory Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Background: Lung cancer is a disease characterized by the uncontrolled growth of the cell in the lung tissue. Considering the significant statistics of lung cancer that accounts for about 26% of deaths compared to total cancers, and current treatments include surgery, chemotherapy, radiation therapy, etc., these commonly mentioned methods Do not provide acceptable returns. Therefore, the use of an effective method with the least side effects and the greatest possible impact is necessary. Nanoparticle (NP)-based combinational chemotherapy has been proposed as a potent approach for improving intracellular drug concentrations and attaining synergistic effects in lung cancer therapy. Here, two wellknown herbal substances, Metformin (MET) and Silibinin (sil), were coencapsulated in PEGylated PLGA NPs and investigated their synergistic inhibitory effect against A549 cancer cells Materials and Methods: Silybin and metformin were loaded onto synthesized nanoparticles. Characterization of nanoformulated drugs was determined using DLS, FTIR, TEM, and SEM. Drug release study was performed using dialysis method. MTT and real-time PCR assays were applied to evaluate the cytotoxic effects of free and nanoencapsulated drugs on expression level of hTERT in A549 cells Results: The results showed that free drugs and formulations exhibited a dose-dependent cytotoxicity against A549 cells and especially, met–Sil–PLGA/PEG NPs had more synergistic antiproliferative effect and significantly arrested the growth of cancer cells than the other groups (p<.05). Real-time PCR results showed that sil, Met and combination of Met–sil in free and encapsulated forms inhibited hTERT gene expression. It was found that Met–sil–PLGA/PEG NPs in relative to free combination could further decline hTERT expression in all concentration (p<.05). Conclusion: In conclusion, it is supposed that nano-encapsulation of Met–sil into polymeric PLGA-PEG NPs may be a convenient drug delivery system to enhance its
59 anticancer effects for lung cancer therapy.
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 72902
Evolution of the expression levels of mir-132 in MCF-7 breast cancer cells treated with Metformin loaded PLGA-PEG NPs, Possible relationship with hTERT expression
Sara Nourozi Dizach1, Mehdi Dadashpour2, Abbas nobakht3, Akram Mohammadi4 Nosratollah Zarghami*5 1. Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran 2. Department of Medical Biotechnology, Faculty of Advance Medical Science, Tabriz University of Medical Science, Tabriz, Iran 3. Research Institute for Fundamental Sciences (RIFS), University of Tabriz, Tabriz, Iran 4. Department of biology, Faculty of Science, Tabriz branch Islamic Azad University, Tabriz, Iran 5. Department of Clinical Biochemistry, faculty of Medicine, Tabriz University of Medical Science, Tabriz, Iran
Introduction: Breast cancer is one of the most common cancers among women today. Currently, miRNAs are considered as attractive targets for therapeutic intervention in cancer prevention and therapy owing to their tumor suppressor or oncogenic action. Emerging evidence has revealed the potent anti-cancer effects of biguanides, Metformin (MET). The aim of the present study was to evaluate the effect of pure MEt as well as the nano-formulation of this drug on the expression of mir-132 and telomerase in MCF-7 cell line. Material and methods: Nanoparticles are synthesized for controlled drug delivery and metformin is loaded onto the nanoparticles. Characterization of nanoformulated drugs was determined using DLS, FTIR, TEM, and SEM. Drug release study was performed using dialysis method. MTT and real-time PCR assays were applied to evaluate the cytotoxic effects of free and nano-encapsulated drugs on expression level of microRNA and hTERT in MCF-7 cells. Results: The results showed that free drugs and nano-formulations exhibited a dose- dependent cytotoxicity against MCF-7 cells cells and especially, MET–PLGA/PEG NPs had more synergistic antiproliferative effect and significantly arrested the growth of cancer cells than the other groups (P < 0.05). Real-time PCR results revealed that MET in free and encapsulated forms inhibited hTERT gene expression. Also, it was found that MET NPs than free forms could further increase Mir-132 expression and decrese hTERT expression in all concentration (P < 0.05). Conclusion: It is speculated that the nanofurmolation of MET may be a promising and convenient approach to improve the efficiency of breast cancer treatment. Moreover, these findings demonstrated that the nanodelivery of Met through targeting miR-132 might be a novel strategy for the treatment of breast cancer
Keywords: mir-132, breast cancer cells, Metformin, PLGA-PEG NPs, hTERT 60 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 82991
Association of rs6065668 C> T polymorphism in Tox2 gene with risk of colorectal cancer
Forough Eidi Khosh1, Maryam Saneipour2, Abbas Moridia3* 1. Department of Science, Faculty of Basic Sciences, Islamic Azad University, Dezful Branch, Dezful, Iran 2. Department of Genetics and Molecular Biology, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran
Introduction: Colorectal cancer (CRC) is the third most common cancer in the world. Genetics accounts for 30% of the causes of CRC.The Tox2 gene that located at chromosomal position 20q13.12, is one of the recently identified genes that play a role in the likelihood of CRC.Meanwhile, polymorphisms of a gene can affect the function of that gene.The aim of this study was to evaluate the frequency and association of Tox2- rs6065668 C> T polymorphism with CRC in CRC patients compared to healthy individuals in Khuzestan population. Materials and Methods: Randomly 2 cc of total blood was collected from 50 patients with CRC referred to Imam Hassan Mojtabacancer treatment center (a charity-based referral cancer center in Khuzestan Province, Dezful, Iran) as case group and 50 healthy individuals as a control group.Blood sampleswere poured into EDTA-anti coagulated tubes and stored at -20 ° C until DNA extraction.In this study, the genotypes were identified using PCR-Sequencing technique for rs6065668 C>T polymorphism. Results: Frequency of CC genotype was higher in colorectal cancer patients than healthy controls. There was a statistically significant relationship between rs6065668 C> T polymorphism and CRC (p-value <0.05). Conclusion: The results of this study showed that rs6065668 C> T polymorphism in Tox2 gene is associated with the risk of colorectal cancer in Khuzestan population. Keywords: Colorectal cancer, TOX2 gene, Polymorphism
61 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 43499
Effects of hydrophobic force on secondary structure of the most effective peptide in Alzheimer's disease
Mahsa Rastegar Moghaddam1 and Mohammad Reza Bozorgmehr2, * 1. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran, 2. Department of Chemistry, Mashhad Branch, Islamic Azad University, Mashhad, Iran [email protected], [email protected]
Introduction & objective: Changing the secondary structure of Amyloid β-peptide (1-42) is one of the most important factors in developing Alzheimer's disease. In other hand, experimental and theoretical studies confirmed that hydrophobic force has major contributors to the changing the secondary structure of amyloid beta peptide. In this work, molecular dynamic simulation used to investigate hydrophobic force effects on secondary structure of N-terminal truncated amyloid beta peptide 1-42.
Material & methods: The first ten residues at the N-terminal of the peptide are polar in nature. These residues are located on the outside of the nerve cell membrane and have random coil structure. Thus, they have high dynamics. Therefore, these residues were deleted to amplify the hydrophobic forces in the peptide. By manipulating the Lennard- Jones potential, the hydrophobic forces increased or decreased. Then, the effect of these changes on the peptide secondary structure was traced. Two force fields GROMOS96 and OPLS-AA were used to perform the calculations. All molecular dynamics simulations were performed by GROMACS software.
Results: The root mean square deviation (RMSD) calculation showed that the simulated systems were in equilibrium. The obtained results based on the RMSD values showed that the structure is more sensitive to OPLS-AA force field changes. The results showed that the use of force field OPLS-AA creates a more disordered secondary structure.
Conclusion: With the strengthening of the repulsive forces in the potential of Lenard Jones, followed by increased hydrophobic forces the Amyloid β-peptide (1-42) secondary structure disorder is further compounded.
Keywords: Alzheimer disease, hydrophobic, molecular interaction, force field
62 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 88852
Comparison of expression levels of hsa-miR-155, INPP5D and IFN-γ level in NK cells isolated from refractory/relapsed or responsive patients with large diffuse B-cell lymphoma (DLBCL) and healthy donors
Nasrin Zare*1, Shaghayegh Haghjooy Javanmard2, Nahid Eskandari3, Valiollah Mehrzad4
1. Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan 2. Department of Physiology, School of Medicine and Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan 3. Department of Immunology, School of Medicine and Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan 4. Department of Hematology and Medical Oncology, Isfahan University of Medical Sciences
Introduction and Objectives: The most common high-grade form of non-hodgkin lymphoma (NHL) is diffuse large B-cell lymphoma (DLBCL). NK cells stimulated by CD16 or IL-12 and IL-18 induces the miR-155 overexpression. Has-miR-155 overexpression targets the Src Homology 2 Domain- containing Inositol 5′-Phosphatase (SHIP-1) as a negative regulator, consequently enhances IFN-γ production. IFN-γ is a critical cytokine for the tumor surveillance, therefore understanding the molecular pathways of IFN-γ expression lead to identifying the potential therapeutic targets for cancer. Therefore, we evaluated whether the expression levels of hsa-miR-155-5p, INPP5D and IFN-γ level are different in the NK cells from patients with DLBCL in comparison with healthy donors. Materials and Methods: Patients were consecutively selected from Seyed-al-Shohada and Alzahra Hospital in Isfahan, Iran. Peripheral blood was obtained from healthy people (n=12) and responsive patients with DLBCL (n=10) or refractory/relapsed patients with DLBCL (n=12). Peripheral blood mononuclear cells (PBMCs) (Ficoll-Hypaque gradient) and then NK cells (NK cell isolation kit, Miltenyi Biotec, Bergisch Gladbach, Germany) were isolated. Levels of hsa-miR-155-5p and INPP5D were analyzed by qRT-PCR. The concentrations of IFN-γ in the culture supernatant were measured with the Human IFN-γ ELISA MAX™ Deluxe (Bio-Legend, San Diego, CA) after 72 hours. Results: We observed the increased expression level of hsa-miR-155-5p (P=0.01 LSD Post-Hoc) and decreased expression level of INPP5D (P=0.017 and P=0.001 LSD Post-Hoc) in healthy donors relative to responsive patients or refractory/relapsed patients with DLBCL. The IFN-γ concentration in the culture supernatants of NK cells from healthy donors was more than responsive patients and refractory/relapsed patients (P=0.05 and P=0.001, LSD Post-Hoc). Conclusion: Decreased expression of hsa-miR-155-5p and IFN-γ might be associated with low NK cell function and impaired response to treatment in patients with DLBCL. Therefore, it is necessary to further evaluate the miR-155-5p- INPP5D- IFN-γ pathway. Keywords: DLBCL, hsa-miR-155-5p, INPP5D, IFN-γ, NK cell.
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 44785
To overcome drug resistance in MDA-MB-231 breast cancer cells via paclitaxel loaded nanoemulsion
Farnoosh Attari1, Habibullah Hazim1, Hasan Rafati2 1. Department of Animal Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran. 2. Department of Phytochemistry & Chemical Engineering, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran.
Introduction & objectives: Paclitaxel (PTX), is considered as one of the most effective chemotherapeutic compounds in the treatment of breast cancer. Nevertheless, one of the main problems regarding this reagent in is the developed drug resistance over time. Since a high percentage of PTX resistance in patients would be detectable at the time of initial diagnosis, it seems necessary to search for a new tactic to bypass the PTX resistance. In the current study, to overcome PTX resistance in breast cancer cells, we have established a new nanoemulsion system containing PTX. Materials & methods: A high-speed homogenization method was employed to fabricate PTX-nanoemulsion (PTX-NE) and for size evaluation DLS technique was used. Next, to examine cytotoxicity effects of PTX-NE on drug-resistant MDA-MB-231 cells, MTT assay was performed. Results: We have employed different concentrations of PTX (10nm, 50nm, 100nm, 200nm, 500nm) on the MDA-MB-231 breast cancer line for 24, 48 and 72 hours to assess their viability. Our results indicated that we could not achieve IC50 concentration even after 72 hours, which was indicative of drug resistance. To overcome this resistance, we have used a noneffective dose of TPGS, an inhibitor of MDR pump, combined with PTX. The data illustrated that combined with TPGS, 500nm concentration of PTX could kill almost 59% of the resistant cells. Moreover, data showed that IC50 concentration with PTX-NE was greatly decreased to 250nm, which was even less than the IC50 value in TPGS-PTX combination treatment. Conclusion: Nanoemulsion performed as a stable drug delivery system for paclitaxel which led to the reduction of PTX concentration. Hence, the severe PTX resistance in breast cancer could be effectively reduced by PTX-NE, which could deliver a potential strategy to treat multidrug resistance in this cancer. Keywords: Drug resistance, Breast cancer, Paclitaxel
64 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 70082
MD simulations of L551Q as a novel carcinogenic PI3Kα mutation
Safoura Ghalamkari 1†, Shahryar Alavi2†, Hamidreza Mianesaz 1, Farinaz Khosravian 3, Amir Bahreini1, 4, 5 *, Mansoor Salehi 1, 3 *
1. Department of Genetics and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran 2. Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran 3. Cellular, Molecular and Genetics Research Center, Isfahan, Iran Medical Genetics Research Centre of GENOM, Isfahan, Iran 4. Palindrome Co., Isfahan, Iran 5. Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, PA, USA
Introduction & Objectives: the p110α subunit of PI3K class IA enzymes is encoded by PIK3CA in which take place a huge number of mutations associated with breast cancer. Here, the mutations in exon 9 of PIK3CA was probed and computationally simulate their function was performed. Material & Methods: mutation detection in 40 breast cancer specimens was done by PCR/HRM and PCR/sequencing. The identified mutations were queried via in silico algorithms to check the pathogenicity. The molecular dynamics (MD) simulations were utilized to assess the function of mutant proteins. Results: Three samples were found to harbor at least one of the E542K, E545K and L551Q mutations of which L551Q has not been reported previously. All mutations were confirmed to be pathogenic and MD simulations revealed their impact on protein function and regulation. The novel L551Q mutant dynamics was similar to that of previously found carcinogenic mutants, E542K and E545K. A functional role for the helical domain was also suggested by which the inhibitory signal of p85α is conducted to kinase domain via helical domain. Helical domain mutations lead to impairment of kinase domain allosteric regulation. Interestingly, our results show that p110α substrate binding pocket of kinase domain in mutants may have differential affinity for enzyme substrates, including anit- p110α drugs. Conclusions The novel p110α L551Q mutation could be considered as carcinogenic mutation similar to previously known mutations (E542K and E545K). Keywords: Breast cancer, PIK3CA, novel mutation, PCR/HRM, PCR/sequencing, MD simulation,
65
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 96496
The role of wild-type p53 in cancer
Ali musa arab1, Fatemeh kazemi1, Zahra kiani peykani1, parmida mosadegh1, Matin mahdian1, Faezeh salimian2, Dr shabnam kermani3
1. Bsc in Molecular Biology, Department of Molecular Biology, Islamic Azad University of Najaf Abad, Najaf Abad, Iran 2. Bsc biochemistry, Department of Molecular Biology, Islamic Azad University of Najaf Abad, Najaf Abad, Iran 3. PhD of genetics, Islamic Azad University of Najaf Abad, Najaf Abad, Iran
Introuduction: p53 protein or guardian of genome is the most famous tumor inhibitor of classic type. since more than 50% of human cancers are due to loss of function within p53 due to mutations. this protein has vital role in genomic stability and Tumor suppression through the induction of apoptosis and seizure of the cell cycle, aging, inhibiton of angiogenesis. Materials and methods: In this review article tried to use data’s of google scholar, pumed, nih to conserned about p53 gene role in treatment of cancers. Results: It is now known distruption of p53 mechanisms by affecting and paying attention to carcinogenic mutations causes growth and Tumors progression. The p53 mutation acts as dominant inhibitor compared to wild type of p53.Wild type of p53 (wt) protein can inhibit oncogene_induced cellular transformation. Conclusions: In the current review article p53 regulatory mechanism and also p53 mediated therapeutic strategies for the treatment of malignant cancers are one of the expected factors, for chemotherapy providing a new strategy is for eliminating the negative effect of. p53 mutation within the (wt).
Keywords: apoptosis, cancer, p53
66 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 10669
Bioinformatics analyses reveal differential expression and potential function of circular RNAs as efficient microRNA sponges in gastric cancer
Sadra Salehi-Mazandarani1, Parvaneh Nikpour1, * 1. Department of Genetics and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran *Corresponding author: [email protected]
Introduction & objectives: Gastric cancer (GC) is one of the most common malignant tumors worldwide. Circular RNAs (circRNAs) are a class of non-coding RNAs that due to their beneficial properties like being usually abundant, conserved and stable, expected to be biomarkers for early diagnosis of different cancers. The most prominent function of circRNAs is their actions as microRNA (miRNA) sponge to regulate target gene expression. The current study aimed to identify differentially expressed circRNAs in GC and evaluate their sponge-related functions. Materials & Methods: Three GC gene expression datasets (GSE78092, GSE83521 and GSE89143) were downloaded from Gene Expression Omnibus (GEO). Using GEO2R tool, differentially expressed circRNAs (DECis) with adj p-value<0.05 and │logFC│>1 were filtered out. DECis existing at least in two datasets and with same direction of gene expression changes were then selected. CircInteractome was explored to find interacting miRNAs. Additionally, DIANA-miRPath was exploited to identify molecular pathways potentially mediated by these interacting miRNAs. Results: Using the cut-off criteria, 287 DECis were identified. Three circRNAs including hsa_circ_0000981, hsa_circ_0008301 and hsa_circ_0021087 were existed at least in two datasets with a decreased expression in tumoral compared to non-tumoral gastric tissues. CircInteractome showed 35 miRNAs are totally interacted with these three circRNAs. A total of 31 statistically significant KEGG pathways were obtained from the analysis of target genes of interacting miRNAs. We found that the predicted target genes of interacting miRNAs are mostly associated with crucial cancer-related pathways like Hippo signaling pathway, viral carcinogenesis, cell cycle, adherens junction and transcriptional misregulation. Conclusion: According to GEO datasets, a comprehensive expression profile of differentially expressed circRNAs was revealed in gastric cancer. Gene enrichment analysis predicted that these circRNAs and their associated miRNAs are involved in multiple cancer-associated pathways. Further investigations are needed to unravel the precise mechanism of action of circRNAs in cancer pathogenesis.
Keywords: Circular RNA, Gastric cancer, microRNA, Sponge
67
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 79358
Evaluation and comparison of C.524G>A mutation detection in TP53 gene by two methods of COLD- PCR/HRM and PCR-Sequencing in different dilutions of DNA mutations
Nastaran bagheri1, Mansour salehi 2,3, Hossein sazgar4 1. Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran. 2. cellular, molecular and genetics research center, Isfahan university of medical science, Isfahan, iran 3. Medical genetics research center of genome, Isfahan university of medical sciences. Isfahan iran 4. Department of biology, faculty of science Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Background and Objectives: According to studies and cosmic database, mutation in TP53 gene is one of the most common somatic mutations in breast cancer which accounts for about 26% of mutations. The C.524G> A mutation is one of the most important somatic mutations in the TP53 gene in breast cancer. One of the reasons for the difficult diagnosis of cancer in early stages is the heterogeneity of tumor tissue. For this purpose, selective amplification and enrichment of small amount of mutated DNA is carried out in the wild- type DNA in COLD-PCR. In this method, by determining and using the critical temperature Tc, mutated DNA fragments are exclusively sequenced and amplified, thereby increasing the amount of mutated DNA in the background of non-mutated DNA. In this study, high resolution melting curve (HRM) method is used to detect and identify the desired mutation. Materials and Methods: SKBR-9 and BT-474 cells were first cultured as mutant and non- mutant cell lines and after DNA extraction, serial dilutions of mutant DNA were prepared by wild-type DNA. All factors of COLD-PCR were adjusted by serial dilutions and sensitivity of COLD-PCR / HRM, PCR / Sequencing, PCR / HRM and COLD-PCR / Sequencing methods for C.524G> A mutation in TP53 gene Evaluated and evaluated. Results: Detection of mutation rate by COLD-PCR / HRM method was possible up to 0.4% dilution of mutated DNA, while PCR / HRM assay was about 6.2% and sensitivity of PCR sequencing method. In this study, the rate of sensitivity of this method was up to 1.5% of the mutated DNA by COLD-PCR prior to PCR Sequencing. Keywords: Breast cancer, TP53 gene, C.524G> A (P.R175H) mutation, COLD-PCR / HRM, PCR Sequencing.
68 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 50107
Evaluation of Grape seed extract (GSE) on PTEN and DACT1 tumor suppressor genes expression, cell cycle and apoptosis in ovarian cancer cell line (OVCAR-3) in vitro
Mansour Homayoun 1, Reza Ghasemnezhad Targhi 2, Ali Honarvar1, [email protected] 1*
1. Department of Anatomy and Cell Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. 2. Department of Radiology, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
Introduction and Objectives: Ovarian cancer is the most common malignancy that leading cause of cancer mortality in women. Grape seed extract (GSE) exhibit anticancer effect against several different types of cancer, we aimed to determine the expression of PTENT and DACT1 tumor suppressor genes using GSE and then investigate the growth rate, proliferation, and apoptosis in ovarian cancer cell line. Materials and Methods: The ovarian cancer cell line (OVCAR-3) was treated with GSE (71 μg/ml) for 24 and 48 h invitro while the untreated group was considered as the control. The cell viability and the apoptosis rate were measured by MTT and flow cytometry, real- time PCR was used to determine expression of PTENT and DACT1 tumor suppressor genes and Downstream genes involved in the cell cycle and apoptosis and protein expression was examined by caspase colorimetric. Results: It was found that treatment of ovarian cancer cells with GSE, increased the expression level of PTENT and DACT1 tumor suppressor genes and also increased caspase3,8 and 9 gene expression(p<0.001). the cell growth inhibited through decrease the expression of cyclinD1 and CDK4 (p<0.001). flow cytometry indicated that GSE increased cell apoptosis in OVCAR-3 cells in a time-dependent manner (p<0.001). also by caspase colorimetric identified that GSE treatment increased caspase-3 protein expression (p<0.001). Conclusions: These results suggest that GSE has anti-cancer effect in ovarian cancer cells by inhibited cell proliferation by inducing cell cycle arrest and also increases apoptosis in these cells. Thus, GSE may be useful as herbal antitumor agent for cancer therapy. Keywords: Grape seed extract, ovarian cancer, tumor suppressor genes, apoptosis
69 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 38892
Selenium supplementation can relieve the clinical complications of COVID-19 and other similar viral infections
Mohammad Fakhrolmobasheri1, Zahra Nasr-Esfahani1, Hossein Khanahmad1, Mehrdad Zeinalian1,2 1. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 2. Ala Cancer Control and Prevention Center, Isfahan, Iran
Abstract: COVID-19 is a highly contagious RNA virus infection which causes severe complications such as acute respiratory distress syndrome(ARDS), fulminant myocarditis, and systemic inflammatory response syndrome(SIRS). Among many suggested treatments, the crucial role of supportive nutrient therapies is almost neglected and is only limited to the use of immune boosting nutrients such as vitamin D, E and C in healthy individuals and no definitive nutrient therapy regimens for COVID-19 patients has yet been recommended. Selenium, taking part in human physiology as selenocysteine is One of the most studied subjects among all nutrient therapies. Altogether, 25 selenocysteine containing proteins (selenoproteins) have yet been explored about half of which do not yet possess a well- described function. Among selenoproteins, proteins active in redox homeostasis, proteins located in endoplasmic reticulum and the serum transporter of selenium are well known. Besides their antioxidant functions, Glutathione peroxidase, thioredoxin reductase and other enzymatic selenoproteins play significant roles in cell signaling pathways and gene expression of leukocytes. studies on selenium supplementation in RNA viruses infections such as coxsackie B and influenza indicated that not only selenium supplementation could boost immune system against the infection but also the antioxidant and anti-apoptotic effects of selenium supplementation prevents severe immune responses. Moreover, studies demonstrated that selenium supplementation could decelerate the viral mutation rate which is a highlighted characteristic of SARS-COV-2 complicating the way through mass population immunization. Clinical studies on critically ill patients, also confirmed the role of selenium supplementation in decreasing the mortality rate. Although the recommended dietary allowance (RDA) for both men and women is 55 μg (0.7 μmol)/day based on researches, in COVID-19 patients with no kidney injury, supraphysiological doses of selenium supplementation up to 800 micrograms/day would be likely well tolerated and may lower the risk of lethal complications in both hospitalized and non-hospitalized patients.
70 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 49911
Molecular Aspects of immunomodulatory and antioxidant effects of vitamin D on prevention of COVID-19 complications
Milad Saadatkish 1, Amir Abbas Shiravi 2, Zeinab Abdollahi2 , Paniz Miar2, Hossein Khanahmad2, Mehrdad Zeinalian2,3* 1. School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences 2. Department of Genetics and Molecular biology, School of Medicine, Isfahan University of Medical sciences, Isfahan, Iran 3. Ala Cancer Control and Prevention Center, Isfahan, Iran
Introduction: SARS-CoV-2, the viral cause of COVID-19, leads to lethal infection with multiple organ damages. Strong data shows a definite correlation between Vitamin D (VD) serum levels and inflammatory disorders. Moreover, these nutritious compounds have antioxidant effects that can prevent organ damages due to oxidative stress. In this paper, we studied the role of VD in the prevention of COVID-19 complications due to its immunomodulatory and antioxidant effects. Description: In patients with COVID-19, Ang II/Ang (1-7) ratio is enhanced. This leads to over-activation of NADPH oxidase and NF-κB upregulation. Also, Ang II over- activation in some pathological conditions, like COVID-19, leads to the upregulation of NOX1 and NOX2 enzymes, resulting in overproducing the reactive oxygen species (ROS) molecules. Several studies shown that VD is closely linked to the T cell immune response, differentiation and activation status of B cells and the development of Natural killer cells during inflammatory responses. Moreover, production of some anti-microbial molecules such as defensins and cathelicidins is also stimulated by VD. VD also has antioxidant effects through modulating the mitochondrial activities, up-regulating of glutathione, glutathione peroxidase, superoxide dismutase, and down-regulating the NADPH oxidase. Discussion and conclusion: VD and its metabolites have immunomodulatory and anti- oxidant effects; So it might be helpful in multi-organ failure due to oxidative stress and cytokine storm induced by SARS-CoV2 in COVID-19. It seems that VD supplementation therapy, at-least in VD deficient patients, have potential protective effects against the COVID-19-induced morbidities; An issue needed to be evaluated at the next well-designed clinical trials.
71 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 49754
Knock down of SIRLNT by Antisense LNA GapmeRs and evaluation of its inhibitory effect on proliferation in human breast cancer cells
Mahboobeh Golchin1 , Mohammad Kazemi2 *, Mohammadreza Sharifi2 , Reza Mohammadzadeh1
1. Department of Cell and Molecular Biology, Faculty of Basic Science, University of Maragheh, Maragheh, Iran 2. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Introduction and Objectives: long non-coding RNAs (lncRNAs) play a critical role in progression of human disease especially in cancer. LncRNAs being potential candidates for therapeutical applications during different diseases. In caccer, lncRNAs could be as either oncogenes or tumor suppressor genes. In the current study, we targeted oncogenic lncRNA SIRLNT (SIRT1 regulating lncRNA tumor promoter) as one of the most significantly up-regulated lncRNA in human breast cancer. Materials and Methods: We used Antisense locked nucleic acid (LNA) GapmeRs oligonucleotides method, which is a potent transduction system to regulate lncRNA SIRLNT expression. MCF-7 cell line was transfected with lncRNA-SIRLNT antisense LNA GapmeRs at three different time points. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to evaluate expression of lncRNA- SIRLNT and SIRT1 (as a molecule under the influence of lncRNA-SIRLNT). The effects of lncRNA-SIRLNT knock down on cell proliferation were explored by 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: The results suggest that lncRNA SIRLNT was significantly downregulated and SIRT1 upregulated significantly after transfection MCF-7 cell line by Antisense LNA GapmeRs. Significantly, we found that the LncRNA SIRLNT knockdown lead to reduction of proliferation of MCF-7 cell line. Conclusion: Our finding suggests that lncRNA SIRLNT inhibition could be a promising therapeutic approach to prevent progression of breast cancer. Keywords: Breast cancer, LncRNA SIRLNT, LNA GapmeRs oligonucleotides, MCF-7, Sirtuin1
72 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 81933
The effect of static magnetic field on mouse pre-antral follicle growth and matured oocyte fertilization in vitro
Niloofar hassani1,2, Rouhollah Fathi2, Mehdi Totonchi3,4, Vida Kazemein Jasemi2, Mohammad Jafari Atrabi2, leyla Tahaee2, Abdolhossein Shahverdi2, Hussein Eimani2,5* 1. Department of Cell and Molecular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and culture, Tehran, Iran 2. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran 3. Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran 4. Department of Genetics, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran 5. Department of Anatomy, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran * Corresponding address: [email protected]
Introduction & Objective: In vitro culture of ovarian follicles is a method to evaluate of folliculogenesis that may be used in assisted reproductive methods. Recent research is aimed to find efficient methods to increase the number of healthy follicles to obtain fertilized oocytes from primary follicles in the culture medium. Our aim this study was to evaluate the oocyte maturation rate during in vitro culture of fresh mouse pre-antral follicles under the static magnetic field (SMF).
Materials & Methods: Pre-antral follicles were isolated mechanically from the ovaries of pre-pubertal mice and distributed in to two groups: 1. CFF: Cultured Fresh Follicles 2. CFF + 1mT: Culture Fresh Follicle + 1mT SMF (10 min). The fresh follicles were incubated in 5% carbon dioxide (CO2) at 37◦C for 12 days and the medium was replenished every other day. In this study, Follicle survival, follicle development and oocyte maturation were assessed during in vitro culture in two experimental groups.
Results: In the CFF + 1mT group, antrum formation rate was higher comparing to the CFF group, whereas follicle survival rate was similar. In the SMF exposured group had higher
oocyte maturation rate than the control group, 2PN formation rate in CFF + 1mT group was higher than in CFF group (P<0.05). Conclusion: It was concluded begin exposed to SMF during in vitro follicle culture leads to an improvement in follicle development and oocyte maturation. Keywords: Pre-antral follicles, Static magnetic field
73 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 98569
Mice oocyte vitrification does not influence fertilization by DNA fragmented sperms but reduces blastocyst formation
Niloofar Khajedehi1,2,3, Samira Hajiaghalou3, Leila Rashki Ghaleno3, Rouhollah Fathi3*, Hamid Gourabi2*
1. Department of Molecular Genetics, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and culture, Tehran, Iran 2. Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran 3. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran * Corresponding address: [email protected], [email protected]
Introduction and Objectives: Nowadays, the vitrification of oocytes widely used in assisted reproductive technology. Some studies show that vitrification can reduce the quality of embryo or blastocysts rate but in general does not have any effect on implantation and live birth rate. We tried to answer this question, is it the same embryo development potential if the oocyte was fertilized with DNA fragmented sperm (DFS). Material and Methods: This ongoing project was conducted on DBA/2 mouse model of sperm DNA damage by intraperitoneal injection of tert-Butyl hydroperoxide (TBHP) at a dose of 203μmol per 100gr weight for 14 days, then the epidydimal sperm were used for in vitro fertilization (IVF). For control, no treatment was done. Cumulus-Oocyte-Complex (COC) was derived from oviducts of female DBA/2 mouse and superovulated with pregnant mare’s serum gonadotropin (PMSG) and continue with human chronic gonadotropin (HCG) after 48h. The COCs were randomly divided for verification and using fresh and conjugated with DFS and intact untreated sperms. For vitrification, COCs were washed and put in 7.5% Ethylene glycol (EG) +7.5% Dimethyl sulfoxide (DMSO) for 5 minutes, then exposed to 15% EG +15% DMSO + 0.5mol/L sucrose for 1 minute and saved in LN2 on Cryotop. For warming, vitrified oocytes were washed with 3 different concentrations of sucrose contain 1mol/L, 0.5mol/L and 0.25mol/L for 1, 3 and 3 minutes, respectively. Results: There was no significant difference in fertilization rate between non-vitrified and vitrified with untreated and DNA fragmented sperms, but blastocyst formation rate decreased in the vitrified COCs compared to the non-vitrified group when fertilized with both untreated and treated sperms. Conclusion: It seems regardless of sperm DNA fragmentation, which may be repaired in the first steps of fertilization, the reduction of blastocyst rate is due to oocyte damage in the process of vitrification per se. Keywords: Assisted reproductive technology, Cumulus-oocyte complex, Vitrification, Sperm DNA fragmentation
74 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 39773
Synergistic anticancer effect of Nano-encapsulated metformin-artemisinin on grows and cell cycle arrest in human breast cancer T47D cell line
Nasim Hassani1, Mehdi Dadashpour2, Leila Khoshravan3, Nosratollah Zarghami*4 1. Department of biology, Faculty of Science, Tabriz branch Islamic Azad University, Tabriz, Iran 2. Department of Medical Biotechnology, Faculty of Advance Medical Science, Tabriz University of Medical Science, Tabriz, Iran 3. Department of, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran 4. Department of Clinical Biochemistry, faculty of Medicine, Tabriz University of Medical Science, Tabriz, Iran
Introduction: Breast cancer is the most common cancer in women. Molecular target therapy is a new treatment, refers to treatment with a new generation of cancer drugs designed to interfere with a specific molecular target, that have a critical role in tumor progression and proliferation. Cell cycle molecules have been considered as molecules target for breast cancer target therapy. Phytochemicals, can either modulate signaling pathway leading to cell cycle regulation or directly alter cell cycle regulatory molecules, can be used in cancer therapy. The low bioavailability and short half-life of this phytochemicals, hamper their application as an anticancer drug. Loading inside PLGA- PEG increase their solubility and drug tolerance and decreases the side effects of the drug. Resent studied suggest that combination of anti-tumor phytochemicals can be more effective in modulating different signaling pathways associated with tumor cell growth. The purpose of this study was to investigate the synergistic antiproliferative effect of combined Metformin and Artemisinin, two phytochemicals, on cell cycle arrest of breast cancer cells. Methods: The PLGA-PEG was prepared by ring-opening polymerization method. The structure of PLGA-PEG was evaluated by using FT-IR and SEM. The cytotoxic effect of individual and combined drugs on T47D cells were evaluated by MTT assay. The cell cycle distribution was analyzed by flowcytometry. Result: The MTT assay and flowcytometry results show that each compound exerted cytotoxic effects on T47D cells, both in time and dose-dependent manner. Met–Art– PLGA/PEG NPs had more synergistic antiproliferative effect and significantly arrested the growth of T47D cells than the other groups. Conclusions: The results provide evidence that synergistic anti proliferative effect of combined Metformin and Artemisinin lead to cell cycle arrest of breast cancer cells and suggest their combination in nanoparticles have therapeutic value in treatment of breast
cancer Keywords: Metformin, Artemisinin n, PLGA/PEG, synergistic effect, breast cancer 75
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 90276
BCL11A enhancer Knock-out: an approach for the treatment of β- thalassemia
Mehdi Hassani1, Mehdi Banan1
1- Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran Introduction: Beta-thalassemia is the most prevalent monogenic and hereditary blood disorder worldwide, created by a mutation in the beta-globin gene. Some experimental studies have suggested that this disease can be treated by using the induction of fetal Hemoglobin (HbF or α2γ2). Hereditary Fetal Hemoglobin (HPFH) is a benign disease in some people with high levels of fetal hemoglobin lifetime. Individuals with compound heterozygous β-thalassemia and HPFH have moderate clinical symptoms. Description: BCL11A is a transcription factor, and the regulator has been demonstrated to be embryonic and fetal globin genes silent in both mouse models and human cells, which regulates fetal hemoglobin (HbF) switching. Binding the BCL11A to the upstream Locus Control Region (LCR) lead to blocking of the interaction between the LCR and the HbF globin gene, which is essential for fetal globin expression. An erythroid-specific intronic enhancer is necessary for erythroid BCL11A expression. Fetal gamma-globin could be reactivated using genome-editing strategies such as ZFN and CRISPR-Cas9, the deletion of critical sequences and knock-out of the BCL11A enhancer in CD34+ Human Hematopoietic Stem and Progenitor Cells (hHSPCs). Discussion and conclusion: Clinical evidence has shown that by increasing the level of fetal hemoglobin, the severity of β-thalassemia could be alleviated. Previously, the targeted deletion of the BCL11A enhancer has been led to reactivation of gamma-globin in Κ562 cells. These findings suggest that the disruption of critical sequences within the BCL11A enhancer defined here may result in HbF levels exceeding a clinical threshold required to ameliorate the β-hemoglobin disorders. In two clinical trials, CD34+ hHSPCs was modified in the BCL11A enhancer by CRISPR-Cas9 and ZFN to treat β-thalassemic patients are currently at the recruiting process. Ultimately, this method may be a potentially curative gene therapy approach for β-hemoglobinopathies. Keyword: BCL11A enhancer, β-thalassemia, Hereditary Fetal Hemoglobin (HPFH), CRISPR-Cas9
76 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 55632
Design and synthesis of polymer particles containing Curcumin on hTERT gene expression in the lung breast cell line (MCF-7)
Akram Mohammadi1, Mehdi Dadashpour2, Sara Nourozi Dizach3, Abas Nobakht4, Nosratollah Zarghami*5 1. Department of biology, Faculty of Science, Tabriz branch Islamic Azad University, Tabriz, Iran 2. Department of Medical Biotechnology, Faculty of Advance Medical Science, Tabriz University of Medical Science, Tabriz, Iran 3. Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran 4. Research Institute for Fundamental Sciences (RIFS), University of Tabriz, Tabriz, Iran 5. Department of Clinical Biochemistry, faculty of Medicine, Tabriz University of Medical Science, Tabriz, Iran
Background: Curcumin has attracted great attention in the therapeutic arsenal in clinical oncology due to its chemopreventive, antitumoral, radiosensibilizing and chemosensibilizing activities against various types of aggressive and recurrent cancers. However, short half-life and weak bioavailability of Cur limited its use as a chemotherapeutic agent. The present study is intended to evaluate the efficiency of PLGA- PEG as a nano-carrier for Cur to increase anticancer effects on MCF-7 breast carcinoma cells. The objective of the current work was to assess the anticancer e ects of Cur loaded PLGA-PEG nanoparticles (Cur- NPs) via downregulation of hTERT against MCF-7 cells. ff Methods: Cur loaded PLGA-PEG nanoparticles were synthesized and characterized by SEM and FTIR. Cytotoxic assays were evaluated in MCF-7 cells treated with the MTT assay. Also, real-time polymerase chain reaction (Real-Time PCR) was used to determine the gene expression levels of hTERT. Results: The spherical shapes of the Cur-NPs with an average diameter size around 190.12nm and a zeta potential value of −17mV were characterized using DLS, and confirmed through FE-SEM. In vitro cytotoxicity assay using MTT revealed that the Cur and Cur-NPs exhibited a dose- and time- dependent cytotoxic e ect against MCF-7 cells (P < 0.05). qPCR findings revealed that the Cur-NPs also doown-regulated the expression levels of hTERT at all used concentrations compared with the drugsff used alone after 48 h treatment (P ≤ 0.05) Conclusions: In conclusion, it is suggested that nano-encapsulation of Cur into polymeric PLGA-PEG NPs may be an e cient strategy to enhance its anticancer effects for breast cancer therapy. ffi Keywords: Curcumin, Breast cancer, Nanotechnology, Nanoparticle, hTERT
77 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 93751
Macrophage repolarization using quercetin-based electrospun nanofibers: possible application in regenerative medicine
Akram mohammadi1, Sara Nourozi Dizach2, Akram Firouzi- amandi3, Mehdi Dadashpour5*
1. Department of Chemistry, Faculty of Science, Tabriz Branch- Islamic Azad University, Tabriz, Iran 2. Department of biology, Faculty of Science, Tehran branch Islamic Azad University, Tehran, Iran 3. Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran 4. Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
Introduction: In the regenerative medicine therapies, the availability of engineered scaffolds that modulate inflammatory states is highly required. The aim of this study was to evaluate the efficiency of electrospun nanofibrous scaffolds containing natural substances with anti-inflammatory properties such as Que to control inflammation and re- polarization of macrophages toward M2 anti-inflammatory phonotype. Methods: For this purpose, bead free and smooth Que-blended PCL/PEG electrospun nanofibrous mats were successfully fabricated and characterized using FTIR, DLS and FE- SEM. MTT assay was used to assess the toxicity of Que -blended PCL/PEG electrospun nanofibrous on LPS/IFN-γ stimulated peritoneal exudate macrophages. To investigate the repolarization e ciency of Que-blended PCL/ nanofibrous, real-time PCR was applied to measure M1(iNOS) and M2(Arg1) markers expression. ffi Results: MTT results showed that macrophage viability on Que -PCL/PEG nanofibers was higher than on PCL/PEG nanofibres and control. Additionally, the presence of Chr into nanofibers was found to influence on macrophage morphologies, using FE-SEM. qPCR results showed a reduction in iNOS-2 and an increase in Arg-1 levels of macrophages seeded on Que -PCL/PEG nanofibres, indicating the successfully polarization of the macrophages to M2 phenotype. The change in macrophage phenotype on Que -based nanofibres could suppress the inflammation in LPS/IFN-γ stimulated macrophages as evidenced by a major reduction in pro-inflammatory cytokine levels TNF- α, IL-1β, and IL-6. Conclusion: The results revealed that Que -PCL/PEG nanofibers-based drug delivery system might be introduced into biomaterials to fabricate bioactive smart multifunctional nanocomposites with macrophage repolarization activities for regenerative medicine purposes Keywords: quercetin, electrospun nanofibrous, Macrophage repolarization, Regenerative
medicine
78
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 55023
Linking cancer genomics to metabolites: A Mendelian randomization study
Z Kamali1, A Vaez1,2 1. Department of Bioinformatics, Isfahan University of Medical Sciences, Isfahan, Iran 2. Department of Epidemiology, University of Groningen, University Medical Centre Groningen, Groningen, the Netherlands
Introduction & Objectives: Breast cancer (BC) is the most common cancer in women and the 2nd most common cancer overall (Bray et al., 2018). Several studies have attempted to unravel the underlying physiology; however, a complete knowledge of the exact etiology is still lacking due to the complexity of the disease. Genome-wide association studies (GWAS) have revealed thousands of association signals influencing complex traits or diseases including BC, providing a great opportunity to unravel causal mechanisms. Here we employ BC GWAS results as genetic instruments (or instrumental variables; IV) and try to investigate the causal associations of circulating metabolites with the risk of BC. Materials & Methods: We worked over the largest GWAS study of breast cancer comprising a total of 122,977 cases and 105,974 controls from European ancestry (Michailidou et al., 2017). We first identified the independent associated loci to BC by clumping GWAS results based on GWAS p- value<5.00E-08, r2>0.05 and physical distance of 1Mb. Next we used GWAS results of 123 metabolites, measured by magnetic NMR, reported by studying 24,925 individuals of European descent (Kettunen et al., 2016). Finally, we integrated BC with 123 metabolites using Mendelian randomization (MR) approach (Zhu et al., 2018). Since MR needs strong genetic instruments to prevent inflation of test statistics under the null hypothesis that bxy = 0, we set the significance threshold of GWAS p-value for genetic instruments to 5.00E-08. After exclusion of two metabolites lacking strongly significant SNPs (p-value≥5.00E-08), 121 metabolites remained which were then clumped and used for MR analysis against BC. We controlled inflation of test statistics due to LD using HEIDI- outlier test. We also checked pleiotropic effects of the used IVs by LD Hub (Zheng et al., 2017) to adjust for potential genetic confounders using mtCOJO approach (Zhu et al., 2018). Results: Bi-directional MR analyses of 121 metabolites against BC, controlling for linkage and pleiotropic effects, revealed significant causal associations for 28 metabolites (P-value < 4.13E-04; which is the Bonferroni corrected threshold based on all metabolite-disease tests i.e. 0.05/121) in forward analysis i.e. metabolite → disease. Tyrosine and total cholesterol in HDL with the largest ORs (1.16 and 1.11 respectively) were at the top of risk factors and total lipids in chylomicrons and extremely large VLDL showed the strongest protective effects (OR = 0.87, P-value = 3.41E-06). Reverse MR analyses i.e. disease → metabolite resulted in no significant associations (P-value > 4.13E-04). Conclusion: Our results suggest that high levels of circulating Tyrosine increase the risk for BC which may be explained via Tyrosine kinase pathways (Hsu and Hung, 2016). It also suggest a protective role for chylomicron and VLDL ingredients against the disease free of non-genetic confounders. Our pipeline can be expanded to include whole metabolome and also can be applied by other researchers
for any other traits or disease. 79 Keywords: Association, breast cancer, lipids, Mendelian randomization, causality.
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 79666
Non-invasive colorectal cancer screening by ITGA4 promoter methylation in peripheral blood mononuclear cells
Sima Jafarpour1, 6, Farideh Saberi1, 6, Guilda Amini1, Rasoul Salehi1, 3, 6*
1Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 2Child Growth and Development Research Center, Research Institute for Primordial 3Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran 6Pediatric Inherited Diseases research center, Isfahan University of Medical Sciences, Isfahan, Iran
Background: Early diagnosis of cancer is necessary for treatment and prevention and can reduce the rate of death due to cancer. ITGA4 is one of the cell surface molecule that its methylated form is important role in many cancers for example CRC. This study aimed to assess the diagnostic role of TIGA4 hypermethylation in cancer especially in CRC. Materials and Methods: In this study 200 CRC patients and 200 normal controls were recruited to measure quantitative methylation of ITGA4 genes in DNA extracted from peripheral blood mononuclear cells (PBMCs) using MethyQESD method. Result: The promoter methylation of ITGA4 gene in cancer group was significantly higher than non-cancer group (mean, 122.4 vs. 99.3, respectively, P<0.05). The sensitivity and specificity of ITGA4 gene for diagnosis of CRC was 54.9% and 60.2 % respectively. Our study demonstrate that ITGA4 methylation might be a valuable biomarker for CRC diagnosis. Key words: Colorectal cancer, screening, MethyQESD
80 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 75768
The study of the protective effect of vitamin E and Retinoic acid on testicular tissue in mice treated with cyclophosphamide
Fatemeh Sadat Moravej* (MD), Shima Toghiani (PhD), Akbar Karimi (PhD) Department of Biology, Payame Noor University, Isfahan Corresponding Author: Fatemehsadat Moravej [email protected]
Introduction: One of the side effects of anticancer drugs is spermatogenesis disorder and the evaluation of sperm production after chemotherapy has been the subject of several studies today. materials and methods: Adult male mice weighing 20 5 5 g were randomly divided into 5 groups (n = 4) Control, cyclophosphamide (12 mg / kg / day), cyclophosphamide and vitamin E (200mg / kg / day), cyclophosphamide and retinoic acid (500 uL / per72h), cyclophosphamide and retinoic acid (250 ul / per72h) and vitamin E 100mg / day ) Were divided. The treatment was continued for 35 days and at the end of the rats were anesthetized and testicular tissue was isolated, weighed, fixed and stained with hematoxylin-eosin. Then, histological studies were performed and data were analyzed by SPSS software and the differences were considered significant (P≤0 / 001). Results: Significant decrease (P≤0 / 001) in the mean diameter of seminal vesicles and epithelial thickness and index of tubular differentiation was observed in the testis tissue of mice treated with cyclophosphamide compared to the control group and in each group. Cyclophosphamide recipient with retinoic acid or vitamin E parameters significantly increased to the level of control group (P≤0 / 001), whereas cyclophosphamide co- administration with retinoic acid and vitamin A had less tissue protective effect. Discussion: Vitamin E and retinoic acid each can offset the adverse effects of cyclophosphamide on spermatogenesis and may be inducers of spermatogenesis, while the combination of these two antioxidants has less efficacy. Keywords: Cyclophosphamide, Vitamin E, Retinoic acid, Spermatogenesis
81 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 63734
Importance of Circulating free DNA as a prognostic and predictive biomarker for recurrent miscarriage in women with subclinical hypothyroidism
Mehri Nazeri1, Houshang Nemati2 1. Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran 2. Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
Abstract: Recurrent miscarriage affects approximately 1% of all couples trying to conceive and is defined as 3 or more consecutive pregnancy losses before 20 weeks of amenorrhoea. Hypothyroidism is a common disease, with an estimated incidence of 0.3% to 0.5% for overt and 3% for subclinical hypothyroidism (SH) in pregnant women. In 50% of recurrent miscarriages (RM) the cause remains unknown. Cell-free DNA (cfDNA) testing revealed to be valuable as a predictive biomarker in several pathological conditions. There are a growing number of studies considering cell-free DNA (cfDNA) as a surrogate marker for diagnosis, prognosis and monitor of patents response rate to treatment. These findings has gain a significant of interest to developing cfDNA for monitoring of recurrent miscarriage. In this review, we first addressed the recent advancements and highlight the current challenges toward the development of cfDNA as powerfull tool against recurrent miscarriage. Then, we focus on the technical quantitative analysis of cfDNA in clinically healthy females and pregnant women with SH. Keywords: subclinical hypothyroidism, SH, recurrent miscarriage, predictive biomarker and cfDNA
82 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 21081
Serial dilutions: A simple and accurate method to achieve genetically homogenous clones from a cell population
Ranjbar M1, Amiri F1, Dianatpour M2 1. Genetics department, Shiraz University of Medical Sciences, Shiraz, Iran. 2. Genetics department, Shiraz University of Medical Sciences, Shiraz, Iran
Objective & introduction: Screening, diagnosis and treatment of genetic diseases have been facilitated by the emergence of gene editing technologies. Although these technologies can introduce mutations in desired locus, but evaluation of polyclonal cells with different indels and off- targets created by these mutations is a major challenge. Therefore, isolation of single edited cells may be necessary for evaluation of specific genotype and ultimately desired phenotype characterization. Multiple approaches have arisen for isolation of individual cells, such as fluorescent- activated cell sorting, serial dilutions, and cloning cylinders. Factors like simplicity, more economical, and the nature of transfected cells (suspension or adherent) are involved in choosing methods for cells isolation. Despite the widespread usage of these methods, there are some limitations, such as costly, requiring a fluorescent reporter for using FACS, and aseptic techniques for cloning cylinders methods. Similarly, serial dilution method is time-consuming and arduous, however, it is preferably used to separate single clone because it merely needs a statistical estimation and pipetting tools. In this study, we investigated a proper serial dilution method to isolate single cells from transfected HEK293 cells population by CRISPR/Cas9 technique. Material and methods: 1) 48-72 hours after transfection, we separated all cell lines in which have received desired vectors with selectable reporter like GFP or puromycin antibiotic. 2) It was prepared a concentration of 106 cells in 1ml growth media in a sterile falcon or 1.5ml microtubes. 3) Diluted cells 1:10, so we had 105, 1o4, 103 and 100 cells in falcon 15 respectively. In the last falcon, we had one cells for every 10μl, so we added 9 ml culture medium to this falcon up to the final volume of 10 ml. At the end of this step, we had one cell for every 100μl in every well of 96 plates. Results: After a week, examination of the cell plate under a microscope showed that approximately 23-30 wells contained single cells and after 14 days, this number would be increased to 40. Our results showed a good pipetting is imperative for better separation of cells. Conclusion: Due to heterogeneity variation in edited cells with genome editing tools, it is essential to examine the cells individually. Despite the limitation such as laborious and timewasting, researchers attribute to choose methods with features like ease of use, cheap, and lack of requirement for sophisticated tools like serial dilution. Keywords: Genome editing tools, single cell, serial dilution
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 55283
Investigating expression of SOX12 and its inhibition effect on induction of apoptosis in human Acute Myeloid Leukemia cell line (KG-1) by Antisense GapmeRs.
1Arezou Dabiri, 2Mohammad Reza sharifi, 3Akram Sarmadi 1. Department of Immunology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 2. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan 81744-176, Iran. 3. Genetics and Molecular Biology Dept. Isfahan University of Medical Sciences. Isfahan. I.R. Iran
Introduction & Objectives: Acute myeloid leukemia (AML) is a heterogeneous hematopoietic malignancy characterized by the rapid accumulation and malignant proliferation of immature myeloid progenitors. It is the most common leukemia in adults with a high incidence and mortality rate especially in elderly. SOX12 is a novel potential target for acute myeloid leukemia however; the role of SOX12 in leukemia remains unknown. Materials & Methods: In this study, SOX12 was blocked by antisense GapmeRs in human Acute Myeloid Leukemia cell line (KG-1). Cells were transected with SOX12 antisense GapmeRs at 24, 48, and 72 h post-transfection. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was accomplished to evaluate the SOX12, Twist1, CASP3 and CASP9 expression. Results: Our results showed that SOX12 inhibition can reduce expression of Twist1. In addition, downregulation of SOX12 in KG-1 cells could induce apoptosis, probably through the upregulation of CASP3 and CASP9. Conclusion: The findings declare that inhibition of SOX12 could be a new target in the treatment of AML and approach treatment based on antisense therapy. Keywords: Acute myeloblastic leukemia, Antisense GapmeRs, SOX12, Twist1, Apoptosis
84 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 91087
Effect of cerium oxide nanoparticles on the expression of inflammatory cytokines in experimental autoimmune arthritis
Elham Naji zavareh, Seyyed Meysam Abtahi Froushani * Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Introduction & Objectives: Rheumatoid arthritis (RA) is an inflammatory and autoimmune disease known as synovial inflammation and joint damage in several joints. Some anti-inflammatory effects of cerium oxide nanoparticles (CeO2-NPs) have been documented in previous studies. This study was performed to evaluate the effects of cerium oxide nanoparticles on the expression of inflammatory cytokines in experimental autoimmune arthritis. Materials & Methods: The study groups consisted of 3 groups of 10 rats: Normal group, Rheumatoid arthritis (RA) group, and a RA group treated with CeO2-NPs (30 mg/kg; daily). Rheumatoid arthritis was induced by Freund's complete adjuvant injection (0.1 ml). Treatment was initiated when the rats showed symptoms of inflammation in the tarsus joint (day 8) and continued until day 28 when the rats were slaughtered. The expression levels of TNF-α, IL-1, IFN-γ, IL-17, TGF-β, and IL-10 cytokines were measured in the foot-pad of the animals by quantitative PCR. Results: Cerium oxide nanoparticles significantly reduced the severity of inflammation of the foot-pad and also improved weight gaining in affected rats. Gene expression levels of TNF-α, IL-1, IFN-γ, IL-17 were significantly decreased in the group received cerium oxide nanoparticles. The expression level of Il-10 was significantly increased in the group received cerium oxide nanoparticles. However, no significant change was observed in TGF-β cytokine levels. Conclusions: Cerium oxide nanoparticles may be used as a useful strategy to improve the inflammation caused by rheumatoid arthritis. Keywords: Rheumatoid arthritis, Cerium nanoparticles, Wistar rat
85 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 66452
The Effect of Decitabine on the Expression and Methylation of the BTG2 in Association with Changes in miR-125b in NALM6 Cell Line
Asma Vafadar (1) (*), Niloufar Nazemoroaya (2), Gholamhossein Tamaddon (3) 1. Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran 2. Department of Basic Science, School of Medicine, Faculty of molecular Genetics, Science and Research Branch, Islamic Azad University, Tehran, Iran 3. Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract: Precursor B-cell acute lymphoblastic leukemia (B-ALL) is the most prevalent pediatric cancer. DNA methylation and changes in the microRNAs expression of are known to be important causes of B-ALL. Decitabine as a DNA methyltransferase inhibitor agent is able to induce hypomethylation in several tumor suppressor genes. Much evidence has proven BTG2 act as tumor suppressor gene in many malignancies. In the current study, the mRNA expression of BTG2 gene using qReal-time PCR in Nalm6 cell line and five patients suffer from ALL with mean age 5.6 years were determined in compare with seven normal healthy donors age and sex matched. Quantitative Real-time PCR analysis revealed that the expression levels of BTG2 gene was significantly decreased in Nalm6 and according to the Methylation specific PCR (MSP) analysis, this gene was hypermethylated in Nalm6. In next step, the effects of decitabine treatment on the methylation and expression of this gene in association with changes in miR-125b expression levels was evaluated in optimal concentration 2.5 µM of decitabine. Our data showed that decitabine is able to restore the expression level of mentioned gene and down regulate expression level of oncomir 125b in Nalm6 cell line. Therefore, it seems that decitabine can be used as a potential drug for the first line treatment of B-ALL patients, but further in vivo investigation is necessary. Keywords: B-ALL, DNA methylation, MicroRNA, Decitabine, NALM6
86 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 21961
Mesenchymal stem cells therapy exerts neuroprotection in mice model of Parkinson's disease
Saeid Bagheri-Mohammadi1,2*, Rasoul Ghasemi1, Rana Moradian Tehrani 3
1- Department of physiology and neurophysiology research center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2- Department of physiology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran2 3- Department of Genetic and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
BACKGROUND: Parkinson’s disease (PD) is the second most common neurodegenerative disorder after Alzheimer’s disease and is expected to impose an increasing social and economic burden on societies as populations age. The ground- breaking developments in stem cell research in the last decade have revived the interest for intracerebral cell transplantation as a therapeutically approach for PD. The experimental research with intranasal administration of stem cells in PD mouse model can work and in some cases induce major, long-lasting improvement. This research was done for evaluating effect of intranasal delivery of HEDSCs in mouse model of PD. METHODS: In this research, the intranasal route was used for administration of human endometrium-derived stem cells (HEDSCs)-GFP labeled in a mouse model of PD in optimal dose (5×104 cells µl-1). During 4 months after cell application, behavioral tests including rotational behavior test, rotarod test, and open field test were examined. In addition, immunohistochemistry was used to assay, GFP, human neural Nestin, and tyrosine hydroxylase (TH) markers in the fixed brain tissue at the substantia nigra pars compacta (SNpc) area. RESULTS: The result demonstrated that administration of HEDSCs can enhanced behavioral parameters compared with control group significantly. The data from immunohistochemistry analysis revealed that the GFP and also Nestin as a differential neuronal biomarker was expressed in SNpc area. Also, TH as a dopaminergic neuron marker significantly increased after cellular therapy in an optimized dose 5×104 cells µl-1. CONCLUSIONS: Our investigation revealed that cellular therapy with HEDSCs improved the PD symptoms in the mouse model of PD as an effective method. KEYWORDS: Parkinson’s disease, Mesenchymal stem cells, Cell therapy, Dopaminergic neurons, Neurogenesis
87 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 47739
Association of rs1342945 and rs12478601 single nucleotide polymorphisms with Polycystic Ovarian Syndrome (PCOS): a case control study and in silico analysis
Leila Naserpoor1, Kambiz Roshanaei2, Nafiseh Hasani3, Mohadeseh Khoshandam4, Naser Kallhor5 1. Reproductive Biology, The Academic Center for Education, Culture and Research, Qom Branch, Qom, Iran 2. Biology Department, Faculty of Science, Islamic Azad University, Qom, Iran 3. department of Cell and Molecular Biology, Faculty of Basic Science, University of Maragheh, Maragheh, Iran 4. Medical Genetic Department, Faculty of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran 5. Department of Physiology, Faculty of Science, Qom Branch, Islamic Azad University, Qom, Iran
Introduction & Objectives: Polycystic ovary syndrome (PCOS) is among the most general endocrine disorders of women, affecting near 5–15% of reproductive age women. The etiology of PCOS remains unknown; although, heritability studies proposed that there is a strong genetic susceptibility to the disorder. A previously presented genome-wide association study (GWAS) of PCOS found that several loci certify evidence. The aim of this study was to investigate the association of two single nucleotide polymorphisms (SNPs) in a case control study of women with PCOS referred to Jahad Daneshgahi infertility treatment center, Qom branch (ACECR). Materials & Methods: For this purpose, two polymorphisms in the THADA gene, which are called SNP (rs)12478601 and rs13429458, were selected from the data obtained by the International haplotype map (HapMap) Project regarding Chinese Han population, and were evaluated by tetra‑primer ARMS‑PCR. To explore possible relationships between SNP and phenotypic variation, different computational tools like SNP Nexus, UTRscan, Human Splicing Finder (HSF), Repeat Masker and RNAsnp Web Server PupaSuite were used for prioritization of high-risk SNP in (interionic and exotics 5’ and 3’-untranslated region (UTR) SNPs). Results: In this study for the first time it was shown that rs13429458 polymorphism is not associated with PCOS risk in Iranian women. On the other hand, data analysis indicated that the rs12478601 genotype significantly increased the risk of PCOS (p≤0.05). Also, prediction analysis of intronic SNP using the human splice finder web tool indicated that rs12478601 induces the abrogation or creation of such binding sites. In silico prediction indicated that it may has an impact on the RNA secondary structure and it located adjacent the signal sequence for poly adenylation. UTRscan demonstrated that this region is located upstream of open reading frame (uORF). Conclusion: Because several studies about PCOS are based on Han ethnic women, studies on women of other ethnic backgrounds and geographical origins are required. Repetition is necessary to determine whether the same variants conferring risk for PCOS in women all around the world. Bioinformatics analysis indicated that rs12478601 in intron may affect cryptic splice site. The molecular basis for SNP in splicing efficiency is largely unknown, but identification of the responsible factors could facilitate the development of therapies. Keywords: PCOs, SNP, ARMS‑PCR, Bioinformatics, splice site
88
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 94435
The potential role of chitosan-based nanoparticles as drug delivery systems in pancreatic cancer
Fatemeh sadoughi1, Mohammad Ali Mansournia2, Seyyed Mehdi Mirhashemi3*, Zatollah Asemi1 1 Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, I.R. Iran 2 Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 3 Metabolic Diseases Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran
Introduction: Pancreatic cancer is one of the most lethal diseases in comparison to other types of cancer occurring in men and women. This cancer is considered as an important global health problem due to its asymptomatic early stages, poor prognosis, high mortality rate, anatomical inaccessibility, and lack of proper diagnostic methods. Consequently, despite all the improvements in the field of cancer diagnosis and treatment, an intense need for more valid and practical procedures exists. Nanotechnology is an inchoate field of science that previously scientist’s tendency to enhance the efficacy of current preventive, diagnostic, and therapeutic methods has oriented them to build a bridge between this science and medicine. Providing a targeted therapy for many kinds of cancer, especially for pancreatic cancer, through the establishment of nanoformulations is one of the aims of nanomedicine. Chitosan is a chitin derivative with many properties that are making it an appropriate option for designing drug delivery systems. Description: This review will present and summarize latest research being carried out on chitosan-based nanoformulations such as nanoparticles (NPs) and liposomes in the field of diagnosis and treatment of pancreatic cancer. It will also highlight the delivery of some common chemotherapeutic drugs such as gemcitabine, paclitaxel, and doxorubicin by the means of these nanoformulations. In addition, this review article will report various studies, where these nanoformulations have been utilized for their therapeutic potential. Conclusion: In summary, multiple empirical explorations have been successful in suggesting the role of chitosan-based nanoformulations in diagnosis and treatment of pancreatic cancer. Folate-chitosan NPs, glycol-chitosan microsphere, O-carboxymethyl chitosan NPs, and lipophilic stearoyl chitosan coated are some of the formulations that can be loaded with chemotherapeutic drugs. Furthermore, there are also some other chitosan nanoformulations which can be used in molecular imaging of pancreatic tumors such as chitosan-coated magnetic NPs loaded with antisense oligonucleutide.
89
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 55762
Study of chromosomal rearrangement in leukemia patients using Karyotype and FISH methods
Zoya Najafi1, 2, 3, Amin Izaditabar1, 2, Mansoor Salehi1, 2, 4, *, Valiollah Mehrzad5, Zhaleh Asadi Fakhr4, Farinaz tabibi1, 2, 1. Cellular, molecular and Genetics Research center, Isfahan, University of Medical Sciences. Isfahan, Iran 2. Medical Genetics Research Center of Genome, Isfahan University of Medical Science, Isfahan, Iran 3. Department of Biology, Science and Research branch, Islamic Azad University, Tehran, Iran. 4. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. 5. Department of Hematology and Oncology, Medical School, Omid Hospital, Isfahan University of Medical Sciences, Isfahan, Iran.
Introduction & Objectives: New reports of Leukemia (all types) in the American Cancer Society have estimated 24,500 deaths and 62,130 new cases per year. Cytogenetics chromosomal abnormalities was reports in cancer cells and also acute leukemia. To investigate chromosomal abnormalities, karyotyping and fluorescence in situ hybridization (FISH) methods performed on referred bone marrow samples. Materials & Methods: According to standard protocol, Bone marrow was cultured with specific culture media (Marromax), Slide preparation and with G.Banding method were stained and analyzed. Fluorescence in situ hybridization (FISH) testing was performed using a panel of Leukemia-associated probes from Metasystem company, to detect t(8;21), t(9;22), 20qter, 20q12, 5p15, 5q31 (EGR1 gene and CDC25C gene), 5q33 (RPS14) and chromosome 7 and 8 centromeres. Results: Karyotype analysis on 207 patients diagnosed leukemia performed in medical Genetics center of Genome, Isfahan, Iran. From these numbers 35 case had chromosomal abnormalities. Among this abnormalities 19 case had Translocations (t(8;21, t(9;22), t(2;10), t(1;1), t(15;17), t(12;16), t(3;3), t(6;11), t(1;7)), 2 case Deletion (Del 7q), 3 case Complex Karyotype, 5 case Aneuploidy and other cases such as (inv 16, MAR). Most abnormality related to t(9;22) and t(8;21) then Monosomy 7 and in 6 cases culture failed. FISH analysis on 48 samples 14 of these cases had chromosomal abnormality. Conclusion: One of the diagnostic ways of chromosomal abnormalities for early detection of cancer is karyotype and FISH methods. Although not all problems with leukemia are chromosomal abnormality, but molecular tests such as FLT3, NPM1, CEPA genes and related panels such as AML, CLL, CML, … help diagnosis. However, bone marrow karyotype is beneficial for diagnosis, using the FISH method, could be useful for clinical monitoring and It also helps with early diagnosis and appropriate treatment process and finally choice of suitable medicine. Keywords: Chromosome abnormality, bone marrow, karyotype, fluorescence in situ hybridization (FISH)
90 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 87872
Sequencing of exon.4 of the LDL Receptor gene in patients with Familial Hypercholesterolemia with the aim of studying possible new mutations
Shima Parviz1, Seyed Javad Hosseini2, Ali Movahed۳ 1 MSc, Department of Cellular and Molecular Sciences, the Persian Gulf University, Bushehr, Iran 2 Assistant Professor, Department of Cellular and Molecular Sciences, the Persian Gulf University, Bushehr, Iran And Department of Biotechnology, the Persian Gulf Research and Studies Center, the Persian Gulf University, Bushehr, Iran 3 Associate Professor, Department of Biochemistry, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
Introduction & Objectives: Familial hypercholesterolemia (FH) is the most common genetic disease in the world and is a dominant autosomal disease, which is characterized by increased plasma concentrations of low-density lipoprotein cholesterol (LDL). Clinical diagnosis of this condition is based on personal and family history, findings of physical examinations and measurement of high cholesterol concentration. Most FH-linked single- gene causes mutations in one of the three LDL, APOB, and PSCK9 receptor genes, of which about 90% are present in the LDLR gene. So far, all of the exons of the LDL receptor gene have been associated with various types of mutations associated with this disease, but because of the highest percentage of mutations in exon number 4 in studies conducted so far in the world, here we are finding and reviewing These exons were found in patients with familial hypercholesterolemia in Bushehr Province to find a possible mutation in these patients. Materials & Methods: In this study, 32 patients were selected based on global criteria for diagnosis of the disease. The genomic DNA of these patients was extracted by the Gene All extractor kit and its quality and quantity was determined by spectrophotometer and electrophoresis on 1% agarose gel. Then, to determine the possible changes in exon 4, the sequence of the studied samples, PCR and its optimization were performed, and then direct DNA sequencing was performed. Results: No mutation was observed in exon 4 in LDLR gene in patients. Conclusion: The results of this study showed that exon 4 in the LDLR gene does not play a role in the FH in the population studied, and possibly other exons or other areas of the LDLR gene or other genes contribute to the disease, or their hypercholesterolemia is due to lifestyle and has no relation to gene mutations. Key words: Familial Hypercholesterolemia, Sequencing, LDLR
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 63099
Application of propensity score in the relationship of watching TV and computer and metabolic syndrome in adolescents: the CASPIAN-III Study
Nafiseh Mozafarian 1, Roya Kelishadi 2, Mohammadesmaeil Motlagh 3, *Mohammad Reza Maracy 4 1. MSc in Epidemiology, Department of Biostatistics and Epidemiology, School of Public Health, Isfahan University of Medical Sciences, Isfahan, Iran. 2. Professor of Pediatrics, Pediatrics Department, Child Growth and Development Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran. 3. Professor of Pediatrics, Pediatrics Department, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 4. Professor of Epidemiology, Department of Biostatistics and Epidemiology, School of Public Health, Isfahan University of Medical Sciences, Isfahan, Iran.
Objective: This study aims to assess the relationship of screen time and metabolic syndrome (MetS) among Iranian adolescents. Methods: In this nationwide study, the propensity score (PS) was used in a matched case- control study design. The data was obtained from 5,625 students aged 10-18 years, who participated in a national school-based surveillance program. MetS was defined according to the criteria of the International Diabetes Federation (IDF). In addition, the continuous MetS score (cMetS) was calculated and the best cutpoint for cMetS was selected based on the receiver operator characteristic (ROC) curve estimate of sensibility and specificity. Data analysis was performed by a conditional logistic regression in 2014. Results: Screen time increased the risk of MetS by 44% with a near significant P- value (P=0.052). The time spent on computer during leisure time was significantly associated with MetS and waist circumference (P<0.05). Moreover, the time spent on watching TV had significant relationship with elevated serum triglyceride levels (P<0.05). Conclusion: The current findings serve as confirmatory evidence on the adverse health effects of prolonged ST, including the association of leisure time computer use with increase in the risk of MetS and excess weight, as well as the relationship of the time spent on watching TV with serum triglycerides levels. Reducing sedentary leisure time activity, notably ST, should be considered as a health priority for the pediatric age group. Keywords: Adolescents, Metabolic syndrome, Propensity score, Screen time.
92 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 44258
The epigenetic mechanisms associated with sleep during childhood
Negin Badihian1, Omid Yaghini1, Roya Kelishadi1* 1. Child Growth and Development Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran. * Correspondence to: [email protected], and [email protected]
Introduction: Sleep is an essential period in human life. It is highly important during childhood as an epoch development and brain maturation occur. The circadian and homeostatic systems regulate sleep. Likewise, the complex interactions of genetic, epigenetics and environmental factors affect sleep mainly by influencing sleep regulating systems. Sleep duration, quality and disturbances are all associated with these factors. Here we aimed to summarize epigenetic mechanisms and factors known to be associated with sleep during childhood. Description: We reviewed original researches assessing the associations between epigenetic mechanisms and sleep duration, quality, pattern or disorders among children and adolescents. Conclusion and Discussion: Sleep is known to affect multiple body organs and systems through epigenetic mechanisms. Different epigenetic mechanisms can regulate sleep a well. Micro RNAs are associated with sleep duration. DNA hyper methylation of specific genes i.e. Forkhead box P3is associated with obstructive sleep apnea in children. Epigenetic mechanisms also play roles in the etiology of sleep bruxism. These mechanisms are especially important in children with neurodevelopmental disabilities. Neural programming, which is critical for neurodevelopment, is regulated through epigenetic mechanisms and having a normal sleep is essentially important in this process. Sleep disturbances are highly prevalent in children suffering various neurodevelopmental disabilities such as autism and Tourette syndrome. It seems that epigenetic modifications, related to sleep regulating mechanisms, may modulate sleep characteristics in children and adolescents and affect the maturation of neural system. Further studies elucidating the exact role of epigenetic mechanismsin sleep regulation during childhood are essential.
93 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 70345
The Role of Epigenetics in Myopia: A Review
Alireza Peyman1, Seyed-Erfan Majidi2, Mohsen Pourazizi1,3, RoyaKelishadi4 1. Isfahan Eye Research Center, Department of Ophthalmology, Isfahan University of Medical Sciences, Isfahan, Iran. 2. Medical student research committee, Isfahan University of Medical Science, Isfahan, Iran 3. Pediatric Inherited Diseases Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran 4. Department of Pediatrics, Child Growth and Development Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran
Introduction: Myopia is one of the most prevalent visual impairments especially in childhood. Regarding global prevalence of myopia, approximately 50% of the global population will suffer from myopia by the end of 2050. Both genetics and environmental factors such as near work and outdoor time play crucial roles in the myopia. Although environmental factors along with genetic predisposition are associated with the increasing prevalence of myopia amongst children, the mechanism through which they act is moderately understood. An epigenetic event such as DNA methylation could be one of the mechanisms through which these environmental factors influence the development of myopia. Description: A considerable amount of research has been conducted on the molecular determinants of myopia. GWAS studies of refractive error have identified more than 100 gene loci with a high degree of concordance between studies. However, there have been very few studies investigating whether epigenetic changes may be involved in the myopia. An animal study showed that eyes with refractive error had a higher frequency of scleral DNA methylation at the COL1A1 promoter and a reduced level of COL1A1 mRNA. In the recent study ofepigenome-wide association study among children, researchers identified five CpG sites that associated with early-onset myopia. The genomic loci for these CpG sites are an intergenic region on 8p23, a region near the ARL1 gene on 12q23.2, and regions within the FGR, PQLC1 and KRT12 genes. In addition, early environmental exposures such as stress, or environmental light, could have an effect similar to air pollution, cigarette smoking or other environmental factor influencing the epigenome in other diseases. Conclusion: Currently there are limited epigenetic studies of myopia risks. Examination of epigenetic markers will be providing novel insights into the etiology of myopia, and modification of reversible, environmental factors would be influence the refractive error outcome.
Keywords: Epigenetic; Myopia; Refractive error; DNA methylation
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 35320
A molecular perspective of Carnitine, in the context of COVID-19
Mohammad Fakhrolmobasheri1, Hossein Khanahmad1, Mohammad Javad Kahlani2, Amir Abbas Shiravi1,3, Seyedeh Ghazal Shahrokh1, Mehrdad Zeinalian1,3* 1. Department of Genetics and Molecular biology, School of Medicine, Isfahan University of Medical sciences, Isfahan, Iran 2. Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Sciences and Technologies, University of Isfahan, Isfahan, Iran 3. Ala Cancer Control and Prevention Center, Isfahan, Iran
Abstract: l-carnitine (LC) is a naturally produced molecule in mammalian cells acting as an obligatory cofactor in fatty acid metabolism. Researches indicate that in a wide variety of diseases, carnitine supplementation would be beneficial. Investigating carnitine supplementation in molecular aspects would lead to a better understanding of the subject. Currently, COVID19 has been converted to a catastrophic pandemic worldwide. SARS- CoV-2 virus targets the ACE2 receptor in the human cells and triggers a chain of deleterious events. In this review, a molecular perspective of L-C is addressed in the context of COVID-19 pathogenesis in which renin-angiotensin system (RAS) is upregulated and the NF-κB pathway is overexpressed. Moreover, a progressive cytokine storm is established. L-C could be beneficial against the oxidative stress effects of Angiotensin II by inhibition of NF-κB and down-regulation of NOX1 and NOX2. An anti- apoptotic and genome-stabilizer function has been determined for L-C through inhibition of pro-apoptotic caspases and activation of PARP-1. L-C is an immunomodulator that downregulates the pro-inflammatory cytokines including TNF-α, IL-6, and IL-1 which could quench the cytokine storm. L-C also can act as a protective agent against cardiotoxicity in COVID19 because of the disturbance in the ACE2-mediated signaling pathway, cytokine storm, pulmonary dysfunction and drug side-effects.
95 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 71879
The effects of n-3 fatty acids from flaxseed oil on inflammatory markers and gene expression related to inflammation and insulin metabolism in patients with gestational diabetes mellitus: a randomized, double-blind, placebo- controlled trial
Amirani E1, Tabassi Z2, Reiner Ž3, Panahandeh I2, Naderi F2, Aghadavod E1, Jamilian M4, Taghizadeh M1, Shafabakhsh R1, Satari M1, Mansournia MA5, Memarzadeh MR1, Asemi Z1*
1. Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran. 2. Department of Gynecology and Obstetrics, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran. 3. Department of Internal Medicine, University Hospital Centre Zagreb, School of Medicine, University of Zagreb, Zagreb, Croatia. 4. Traditional and Complementary Medicine Research Center, Arak University of Medical Sciences, Arak, Iran. 5. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Abstract: The present study was performed to evaluate the effects of n-3 fatty acids from flaxseed oil on inflammation and genetic profiles in patients with gestational diabetes mellitus (GDM). This randomized, double-blind, placebo-controlled clinical trial was performed in sixty women with GDM. Participants were randomly divided into two groups (30/each group) to intake either 2 × 1000 mg/d n-3 fatty acids from flaxseed oil containing 400 mg α-linolenic acid in each capsule (n=30) or placebo (n=30) for 6 weeks. N-3 Fatty acid intake upregulated peroxisome proliferator-activated receptor gamma (PPAR-γ) and (P < 0·001) and low-density lipoprotein receptor (P = 0·004) and down-regulated gene expression of tumor necrosis factor-α (TNF-α) (P = 0·001) and interleukin-1 (IL-1) (P = 0·002) in peripheral blood mononuclear cells of subjects with GDM. N-3 Fatty acid administration was also associated with a significant reduction in C-reactive protein (P = 0·006) and an increase in total nitrite (P < 0·001) when compared with the placebo. In conclusion, n-3 Fatty acid supplementation for 6 weeks to women with GDM had beneficial effects on gene expression related to insulin, inflammation and inflammatory markers. Keywords: Flaxseed oil; Gestational diabetes; Gene expression; Inflammation; Insulin resistance.
96 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 47484
Evaluation of the properties of the wound dressing film of chitosan / polyvinyl alcohol-based containing human adipose-derived stem cell exosomes, Silver nanoparticles and sildenafil citrate
Ali Samadi1, Saeed Azandeh1,*, Mahmoud Orazizadeh1, Vahid Bayati1, Mohammad Rafienia2, Masoud Ali Karami3, Akram Gavahi1 1- Cellular and Molecular Research Center (CMRC), Department of Anatomical Science, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences (AJUMS), Ahvaz, Iran 2- Biosensor Research Center, Isfahan University of Medical Sciences, Isfahan, Iran 3- Nanotechnology Research center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Objective: Burn wounds are bad prognosis wounds. These wounds have a lot of discharge and regular dressings are not very useful for dressing these wounds. Hydrogels, with plenty of water and the ability to exchange materials, are the best choice for dressing for these wounds. Exosomes as extracellular vesicles play an important role in intercellular signaling and stimulating cell growth Recent studies have shown that sildenafil citrate plays an important role in angiogenesis and wound healing. In this study, we succeeded in making a new hydrogel dressing by combining many findings on wound healing as well as the use of regenerative medicine. Materials and Methods: In this study, briefly, First, several different compounds were made from chitosan-PVA and their properties were investigated and the optimum composition was determined. FTIR, contact angle, tensile strength Biodegradability, Water absorption, and Water vapor passage test has done. Silver nanoparticles were synthesized from silver nitrate and added to the compound. Exosomes Extracted from Human Adipose- derived Stem Cells and Characterized. The raw material of Viagra (sildenafil citrate) was obtained from drug companies and made a 10% by weight suspension. Then dressing with different compounds was placed on 4 groups of Wistar rats then Tissue samples were examined by H&E, Silver and Masson's trichrome staining Results: Optimal dressing ratio was found. Silver nanoparticle dressing had better antibacterial properties. Real Time pcr for nanog and oct4 genes confirmed that the cells extracted from tissue are stem cell. Western blot analysis confirmed the existence of exosomal markers. A new method was discovered to prepare exosomes for TEM imaging and the rats recovered well. The results in the dressing group showed marked improvement. Conclusion: This combination of tissue engineering, stem cells, nano technology has opened a window of hope to patients with burns bright results were observed in wound healing.
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 89843
Genetically engineered mesenchymal stem cells: targeted delivery of immunomodulatory agents for tumor eradication
Shima Rahimirad1, Samira Rahimirad2, Rasool Salehi1 1. Department of Genetics and Molecular biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. 2. Medical Genetic Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Introduction: Cancer immunotherapy emerged as a novel therapeutic option making use of enhanced or amended native immune system to create a robust response against malignant cells. Cancer vaccines, monoclonal antibodies (mAbs), cell therapy and non- specific cancer immunotherapies with adjuvants are all produced encouraging results either as prescribed drug or at clinical trial phase or as an active research field. The systemic therapies with immune-stimulating cytokines have resulted in substantial dose-limiting toxicities. Targeted cytokine immunotherapy is being explored to overcome the heterogeneity of malignant cells and tumor cell defense, with remarkable reduction of systemic side effects. Description: Cell based strategies, such as dendritic cells (DCs), fibroblasts or mesenchymal stem cells (MSCs) seek to minimize the numerous toxic side effects of systemic administration of cytokines for extended periods of time. The usual toxicities comprised vascular leak, hypotension and respiratory insufficiency. Natural and strong tropism of MSCs towards malignant cells made them an ideal systemic delivery vehicle to direct the proposed therapeutic genes to the vicinity of a tumor where its expression could evoke an immune reaction against the tumor. Compared with other methods, the delivery of cytokines via engineered MSCs is a more practical, safe, and promising strategy. Large numbers of genes code for cytokines have been utilized to reengineer MSCs as therapeutic cells. This review highlights the recent literature on cytokine gene therapy to confront human malignancies with focused on MSCs application in cancer immunotherapy. Conclusion: Taken together, these studies introduce engineered MSCs as a new research field for cancer treatment. Their utilization will be of great benefit to future cancer patients. Keyword: Mesenchymal stem cells, immunotherapy, cancer, cytokine
98 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 57393
Application of whole exome sequencing (WES) in clinical diagnosis; An example
Seyyed Saleh Hashemi1,2, Davood Zare-Abdollahi1, Kolsoum InanlooRahatloo3, Fardad DanaeeFard1, Mohammad Rohani4, Afagh Alavi1* 1. Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran 2. Student Research Committee, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran 3. School of Biology, College of Science, University of Tehran, Tehran, Iran 4. Department of Neurology, Hazrat Rasool Hospital, Iran University of Medical Sciences, Tehran, Iran
Introduction & Objective: Clinical heterogeneity in some neurological diseases makes a significant challenge in the correct diagnosis. The definite diagnosis of these heterogeneous diseases sometimes depends on identification of the causing-genes. On the other hand, genetic heterogeneity may also be observed in this group of diseases. Thus, identification of causative gene by direct sequencing is time consuming and expensive, and needs to a high-throughput method. Whole-exome sequencing(WES) is a powerful diagnostic tool for these cases. Considering the importance of early diagnosis and medications in treatable diseases, selection of a diagnostic test is very important. Herein, we emphasized the potentials of WES in diagnosis in a consanguineous family with Primary COQ10-deficiency. Materials & Methods: An affected individual was referred to us as a suspected-hereditary spastic paraplegia (HSP) case. DNA was extracted and sequenced on the Illumina HiSeq4000 platform. Sequences were analyzed. Non-synonymous exonic, exon-splicing and splicing variants with minor-allele-frequency (MAF)<0.01 were selected. Only homozygous variants that segregated with disease status, further considered as causes of disease. Results: Among the candidate variants observed was c.332T>C:p.(Leu111Pro) in the COQ7 gene that co-segregated with disease status. This gene is known to be causative of the very rare disorder COQ7-associated-COQ10-deficiency, while the proband was referred as a HSP case. She only presented muscle weakness and spasticity in her lower-limbs and a mild hearing impairment at the ages of 3 and 8 years, respectively. There is no metabolic/multisystem involvement that typically observed in Primary COQ10-deficiency. Conclusion: Due to the presence of some overlaps between neurological diseases, clinically mis- diagnosis can occur. Our case initially was diagnosed as HSP but, genetically confirmed as a primary COQ10-deficiency case. Thus, genetic approach may be helpful to distinguish this disease from other similar conditions at earlier ages. Particularly, given that some features of primary COQ10-deficiency may be ameliorated by exogenous COQ10. Keywords: Primary COQ10-deficiency; Hereditary spastic paraplegia (HSP); Whole-exome sequencing (WES); COQ7
99 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Oral ID Code: 43909
Blastocoel Fluid Biopsy As A Non-invasive Preimplantation Genetic Testing Method
Malihe Najaflu1, Mohammad Javad Kahlani2, Mansoor Salehi1,3,4
1.Genetics and Molecular Biology Dept. Isfahan University of Medical Sciences. Isfahan. I.R. Iran 2.Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Sciences and Technologies, University of Isfahan, Isfahan, Iran 3. Medical Genetics Research Center of Genome, Isfahan University of Medical Sciences, Isfahan, Iran. 4. Cellular, Molecular and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
Background: Preimplantation genetic testing (PGT) is a widely used technology for aimed embryo tests that assess the genetic health of embryos produced by in vitro fertilization (IVF). The main challenge of IVF is to select the single best embryo which has the greatest implantation potential. In PGT, there are three types of biopsy for obtaining embryonic material: Polar body (PB), blastomere and trophectoderm (TE) biopsy. PB biopsy causes paternal genome exclusion. Blastomere biopsy has effect on embryo development. TE biopsy may not be representative of inner cell mass. Both TE & blastomere biopsy decrease implementation rate. Methods: When blastocoel fluid (BF) is withdrawn before vitrification by micro-suction technique, the blastocyst survival rate is significantly increased. Investigation of BF led to the identification of an invaluable source of genetic information such as cfDNA, miRNAs and mitochondrial DNA. The biological significance of fragmented DNA is unknown. In this regard, there are three hypotheses: cell- to-cell communication, apoptosis and shedding of abnormal cells by the embryo. Assessing the isolated embryonic DNA from BF biopsy was used for non-invasive preimplantation genetic testing such as genotype demonstrations, chromosomal aneuploidy and monogenic diseases detection and even sex determination. Numerous studies have obtained significant concordance by comparing the results of PB, blastomere and especially TE biopsies with BF. In a study of blastocyst culture conditioned medium combined with BF the concordance rate was much higher, even in some cases it was able to correctly report aneuploidy while TE biopsy failed. Also, another study confirmed the presence of microRNAs in human BF biopsy and found interesting results, including involvement of these microRNAs in cellular signaling pathways, apoptosis which specifically exist in BF that are not expressed in oocytes and spermatozoa. Conclusion: These data indicate that BF contents play an important role in embryo development, this genetic information can be used as a non-invasive method for screening and diagnosis of diseases, as well as finding a way to further understanding the fetal developmental pathways and
how the embryonic cells communicate with themselves and with endometrium. Keywords: PGD, Blastocoel Fluid Biopsy, Genetic Anomalies, Embryo Development
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
101 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 10425
The application of magnetic particle imaging in targeted cancer treatments
Farzaneh Ghorbani1,2, Alireza Montazerabadi2* 1. PhD student of Medical Physics, Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran 2. Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Introduction: In 2005, a completely new quantitative imaging method called magnetic particle imaging (MPI) was introduced, which uses the nonlinear re-magnetization behavior of magnetic nanoparticles (MNp) to determine their local concentration. This article aims to introduce MPI and its applications to the Iranian scientific community. Then quantitative drug release, as one of the clinical applications of MPI, is discussed.
Description: MPI relies on the response of MNp, which can be used as tracers for medical imaging, to an alternating magnetic field. It provides a unique combination of features including high sensitivity, spatial and temporal resolution, inherent quantitative and no ionizing radiation; which makes it a promising method for several clinical applications. Apply MPI by using drug-loaded MNp would enable tracking the biodistribution of the drug. In the main article, the properties of usable MNp in MPI are introduced. Discussion and Conclusion: MPI is well suited for applications that can use tracer materials flowing in the blood stream for a certain amount of time including diagnosis and assessment of cardiovascular disease. The other applications of MPI are in oncology, sentinel lymph node imaging, hyperthermia, cell labeling and tracking, gastrointestinal imaging and lung imaging. Furthermore, specific ligands can be loaded on MNp for targeted imaging as well as chemotherapeutic drugs for targeted therapy. Furthermore, drug release has been monitored using imaging modalities including optical imaging, photoacoustic imaging and magnetic resonance imaging (MRI). However, the penetration depth of optical imaging and photoacoustic imaging in biological tissues is limited. Also, MRI is not linearly quantitative and intrinsic/background signals can convolute drug distribution signals, making it generally unsuitable for quantitative clinical measures of drug release. MPI is likely an ideal imaging modality to monitor drug release because it offers large imaging depths and a linearly quantifiable signal, high sensitivity, low magnetic fields, and real-time imaging capability.
102 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 10441
Evaluation of changes in enzymes of MDA, GPX, SOD, catalase and hepatocytes of male offspring of vitamin E treated rats
Sajjad Kooshki1, Mohammad Taghi Ghorbanian2, Iran Goudarzi3 1. M.Sc. Student in Cell Biology, Faculty of Biology, Damghan University 2. PhD of Anatomy, Faculty of Biology, Damghan University 3. PhD of Physiology, Faculty of Biology, Damghan University Corresponding author: [email protected]
Objectives: Vitamin E can provide a good antioxidant, according to scientific evidence regarding the pharmacology and medicinal properties of the liver at different doses. Vitamin E can play an important role in healing liver damage, so we evaluated the extent of changes in liver antioxidant enzymes and liver tissue. materials and methods: Adult male and female Wistar rats weighing 220 20 20 g were used, divided into six experimental groups, and received oral gavage from day zero of gestation until 28 days after birth. And a number of offspring were anesthetized on day 28 after birth and liver was removed for histopathological evaluation and activity of antioxidant enzymes, MDA, GPX, SOD, catalase assay and stored at -80 ° C. Microtomes were cut to about 5 microns in thickness and stained by H&E method and studied by light microscopy (magnification 400). Data were analyzed by SPSS software at significant level (P <0.05). Results: In this study, oxidative enzymes showed a significant decrease in MDA in vitamin E groups compared to the control group (P <0.05) and GPX and catalase levels also increased significantly (P <0.05). And in histopathological studies, the number of hepatocytes in the doses of 100, 200 and 400 vitamin E significantly increased compared to control, NAVE and oil groups (P <0.05). Conclusion: Vitamin E has a beneficial effect on oxidative stress and reduces liver cell toxicity. It also reduces the damage of liver cells, lobules and hepatocytes. Keywords: Liver, Vitamin E, Antioxidant, Rat, Histopathology
103 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 11188
Circulating cell-free DNA as a prognostic and predictive biomarker for IVF outcome in Polycystic Ovary Syndrome females
Houshang Nemati1, Mehri Nazeri2, Ahmadreza Farmani 1. Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran 2. Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran 3. Tissue Engineering and Applied Cell Sciences Department-school of Advanced technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Polycystic ovary syndrome (PCOS) is the most common endocrinopathy in females of reproductive age, with an occurrence of up to 10 percent globally. Additionally, women with PCOS are at growth risk of infertility. Recognizing biomarkers associated with treatment response and predictive may be effective to improve the outcome of IVF in PCOS cases. Circulating cell-free DNA (cfDNA) is found normally in plasma or serum as a result of apoptosis. Recently, it is found to be present in follicular fluid as an outcome of apoptosis of follicular cell. Several reportes has been performed to investigate the potency to use it as an index of the quality of oocyte and embryo in the in vitro fertilization manners (IVF) to have a better and higher success rate, since IVF is presented as an expensive technique and it is the final hope for treatment of infertility. These findings has gain a remarkable of interest to developing cfDNA for monitoring of IVF outcome in PCOS females. In current review, we first addressed the advancements and highlight the current challenges toward the development of cfDNA as promising tool for outcome of IVF in PCOS females. Subsequently, we focus on the technical quantitative analysis of cfDNA in clinically healthy cases and PCOS patients for outcome of IVF. Keywords: Polycystic Ovary Syndrome, PCOS, IVF outcome, predictive biomarker and cfDNA
104 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 12225
Analysis of the ligand transport pathway through a eukaryotic transporter using Caver Web server
Parinaz Parsi and Hamid Mahdiuni*
Bioinformatics Lab., Department of Biology, Razi University, Kermanshah, Iran
Introduction & Objective: Protein tunnels and channels are key transport pathways that allow ligands to pass between proteins' external and internal environments. These tunnels are attractive targets for drug design. An important challenge we encounter is how ligands access to their binding site in the corresponding channels. Caver Web is an interactive web server suitable for comprehensive analysis of protein tunnels/channels as well as the ligands’ transport. In this study, we used Caver Web to detect and characterization of the channels within the cationic amino acid transporter 1 (CAT-1) and analyzed the transport of a series of hit compounds through the main channel. Materials & Methods: CAVER Web version 1.0 was used to calculate the tunnels and channels in the protein structure of the CAT-1 which was previously modeled using MODELLER 9.20 software and equilibrated in membrane system using GROMACS package for 1 µs. Subsequently, the 15 top hit compounds selected based on the docking energy were subjected to analysis through the identified channel. Results: About 32 tunnels and channels were identified within the cationic amino acid transporter 1. The energy profiles and trajectories for the passage of the ligands through the main channel were calculated. We found out that the compound with Drug Bank ID 07102 and Zinc ID 40916505 have the easiest access to the binding site of the main channel. Conclusion: Considering the findings obtained here, it can be concluded that the binding strength of the ligands to the binding sites is not enough criterion in the drug discovery projects. But, the easiness of access to the druggable site for the hit compounds has significance equal with binding free energy to the binding site. Keywords: Protein tunnels, Caver Web, CAT-1, hit compounds
105 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 12529
Application of Piezoelectric nanobiomaterials in regenerative medicine and cancer therapy
Ahmad Reza Farmani 1*, Maedeh Mohammad Salehi 2, Jafar Ai 1, Sadegh Mohammadi 3 1. Tissue Engineering and Applied Cell Sciences Department-School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. 2. Pharmaceutical Chemistry Department, Pharmaceutical Science Branch Islamic Azad University, Tehran, Iran. 3. Department of Plastic Engineering, Faculty of Polymer Processing, Iran Polymer and Petrochemical Institute, Tehran, Iran.
Introduction: Piezoelectric materials are special kinds of materials which they exchange mechanical stress to electrical charge and vice versa. Due to their unique properties, piezoelectric materials have been founded extremely vast applications in the fields of electrical, ultrasonic, robotics, energy conversion, medicine, space, domestic industries, and many others. Description: owing to the polar nature of proteins and other structural components of cells, electrical field can use as a special tool for changing stem cells fate. Also, researches had shown previously that stem cells react to the mechanical stimulation through their receptors, which this phenomenon called as mechanotransduction. Accordingly, using piezoelectric materials in regenerative medicine can create new opportunities for preparing smart materials. Also, researches showed that by applying nanotechnology for building new biological structures, they can improve their biofunctionality of the engineering of tissues and also cancer-targeting systems. Accordingly, by using piezoelectric nanomaterials the ability for accelerating stem cell differentiation maybe prepared. Also, they can activate by both electrical and mechanical stimulation for guiding stem cells to our desire functional mature cells or releasing drugs for overcoming cancer cells. Discussion and conclusion: piezoelectric nanobiomaterials are multifunctional nanobiomaterials, which can be applied for advanced smart systems in tissue engineering scaffolds and cancer targeting systems. Keywords: piezoelectric, nanobiomaterials, tissue engineering, cancer therapy.
106 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 13357
Designing a potent epitope based vaccine against brucellosis using bioinformatics approaches
Zahra Yazdani1, AlirezaRafiei1, Mohammadreza Yazdani2 1. Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran 2. Department of Chemistry, Isfahan University of Technology, Isfahan, 84156-83111, Iran Corresponding author: Alireza Rafiei
Background: Brucellosis is a one of the most important zoonosis that caused by Brucella bacteria. Brucella abortus is a common Brucella species associated with Human‐to‐human transmission. Therefore, having a potent vaccine against this bacterium in human will be promising. Therefore, we designed a peptide based vaccine according to outer membrane lipoproteins of Omp 10 and Omp 16 proteins using immunoinformatics approaches. This vaccine provides physicochemical properties such as stability in room temperature, potential of antigenicity, non-allergic properties and no requirement with eukaryotic host system. Also, this vaccine can interact with TLR-4. Results: The designed vaccine has two epitopes which promotes cellular immunity and INF-γ responses. In order to increase immunogenicity of designed vaccine, HBHA as a toll like receptor 4 (TLR-4) agonist incorporated in vaccine structure. Structure of the vaccine was predicted using I-TASSER and refined in GalaxyRefine. In the next step, this refined structure was validated using Rampage and ERRAT and a high quality 3D structure of the vaccine protein was provided. The physicochemical properties and potential for expression in E.coli was evaluated using many servers. Its interaction with TLR-4 evaluated with cluspro docking. Conclusions: The vaccine is expected to have a high potential to induce in cellular immune responses, high quality structure and suitable properties including high stability, solubility and a high potential expresseion in E.coli. Therefore, this vaccine may be considered as an anti- Brucella abortus vaccine. Keywords: Bioinformatic Vaccine, Brucella abortus, Tertiary structure analysis, in silico cloning
107 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 13411
Bioinformatics strategy for Determination of microRNAs and Genes Involved in Pathogenesis of Aneurysm in Patient with Autosomal Dominant Polycystic Kidney Disease (ADPKD)
Razieh Fatehi
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Introduction: Autosomal dominant polycystic kidney disease (ADPKD) is caused by mutations in polycystic kidney disease-1 (PKD-1) and PKD-2 genes. The products of these genes, polycysin-1 (PC-1) and PC-2 are expressed as a mechanosensor in the surface of epithelial and endothelial cells, as well as vascular smooth muscle cells (VSMCs). Although cysts in kidneys are common symptoms for diagnosis, cardiovascular problems and cerebral and aortic aneurysms are the main reasons for sudden death in patients. In this study, using bioinformatics approaches, critical genes, microRNAs (miRNAs), and pathways involved in the pathogenesis of aneurysm in ADPKD were investigated. Methods: For microarray analysis, the GSE74451 dataset was downloaded from the GEO NCBI database. The quality of the dataset was assayed by principal component analysis (PCA) using R software. Using the NetworkAnalyst database, the genes expression of ADPKD was compared with normal samples. Also, the gene regulatory network (GRN) based on gene- miRNA interaction was retrieved by the validated interaction from TarBase and miRTarBase databases. The NetworkAnayzer tool and MCODE plugin of Cytoscape software were used for topology and cluster analysis. Using ClueGo of Cytoscape software, Gene Ontology (GO) and functional analysis were performed. Results: Network analysis identified crucial miRNAs and genes in the network with 5407 nodes. The central nodes were determined based on degree, betweenness centrality, closeness centrality, and eccentricity and module analysis. The gene regulatory network analysis resulted in SOD2, IGF1R, MYC, GATA6, and DDX3X are critical genes and hsa- mir-4284, hsa-mir-130b-3p, hsa-mir-26b-5p, hsa-mir-335-5p have the highest centrality parameters and are known as key regulators of the network. Functional analysis was shown key paths mainly related to the regulation of cell growth and differentiation. Conclusion: The study provided high-throughput approaches to explore the main molecular mechanisms of development the vascular complications in ADPKD. Keyworld: ADPKD, Aneurysm, Microarray analysis, Gene Regulatory Network
108 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 13493
Evaluation of expression of CDR1, CDR2 genes in clinical isolates of Candida albicans resistant and susceptible to fluconazole in patients under chemotherapy in Ahvaz
Mehrnoosh maherolnaghsh1, Parvin Dehghan2, Mahnaz Fatahinia3, Ali Teimoori4 1. Ph.D,student of medical mycology, Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical. 2. Assosiate professor, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. 3. Ph.D., Assistant professor of Medical Mycology Department of Medical Mycology, School of Medicine, Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 4. Assosiate professor, Department of Virology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan Iran.
Introduction: Nowadays candidiasis is being into new treatment problem. Resistance to azole drugs during prolonged use and poor response were seen to treatment in candidiasis. The effect of azole drugs on Candida albicans isolates is controlled by several genes, including the genes. They have different molecular mechanisms to resistance azoles drug. In this study, 12 susceptible isolates and 12 isolates of fluconazole resistant Candida albicans were evaluated by qPCR, as a rapid and sensitive assay, to find overexpression of the mentioned genes along with drug sensitivity test. Materials and Methods: The Candida species were obtained by swabbing the oral mucosa of cancer patients in Ahvaz. The isolates were identified by morphotyping and molecular methods. Then, RNA extraction and cDNA synthesis was carried out in 24 Candida albicans isolated from patients. Antifungal susceptibility tests were conducted using micro dilution broth method. MICs were determined after 24 h of incubation at 35 °C and interpreted according to (EUCAST) breakpoints. Primer blast software was used for primers designed for CDR1, CDR2 and ACT1 (internal control) in RT- qPCR. RT- qPCR amplification of reference genes and target genes was performed by using ABI Step One®. Results: In total, 74 Candida isolates were diagnosed from patients. After 24h 21.6% of the isolate were resistant to fluconazole, 10.8% of the isolate were dose-dependent and 67.7% of the isolate were sensitivite. Resistant isolates were identified significantly with over expression in CDR1 gene. Compared CDR2 gene between clinical isolates of fluconazole-sensitive and resistant Candida albicans, did not show significant overexpression. Conclusion: It is important to identify the mechanism of resistance to azole drugs and to know the epidemiology of Candida isolates in clinical laboratories. Rapid molecular methods such as RT- qPCR to identify mechanism of resistance to fluconazole in under chemotherapy patients can significantly affect treatment management of these patients. Keywords: Oral candidiasis, Chemotherapy, MIC, RT- qPCR, CDR1, CDR2
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 13689
Effect of hydroalcoholic and aqueous extract of Dracocephalum kotschyi on belomycin pulmonary fibrosis
Zahra Mirdamadi1, Hassan Sadraei1, Ali Hussiny-Sharifabad1, Mohamed Hashminia2, Seyed Ebrahim Sajjadi1.
1. Isfahan Pharmaceutical Sciences Research Centre, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran. 2. Department of Pathology, Faculty of Veterinary Medicine, Razi University, Kermanshah, I.R. Iran.
Introduction: Dracocephalum kotschyi is an Iranian traditional medicine with anti- inflammatory properties. The anti-inflammatory activity of D. kotschyi extract is attributed to its components apigenin and luteolin. In animal model of fibrosis, it has been shown that apigenin and luteolin prevent fibrosis in animal model. The objective of this research was to investigate preventive effect of D. kotschyi extract in bleomycin induced fibrosis. Method: Fibrosis was induced by intratracheal administration of single dose of bleomycin (5mg/Kg) in rat. Rats were divided in eight groups (n=6). One group received daily administration of normal saline. Other groups were treated daily with oral administration of either hydroalcoholic or aqueous extracts of D. kotschyi (20mg/kg, 40mg/kg, 80mg/kg). Another group was treated with pirfenidone (100mg/kg). After 4 weeks daily treatments, the animals were sacrificed and whole lungs were dissected out for biochemical and histopathatological examination. The biochemical examination included assessment of hydroxyproline (HP) and malondialdehyde (MDA) levels. Data expressed as mean±ESM and compared with control group using Student's t-test. This project was confirmed by the ethical animal committee of the university. Results: Intratracheal instillation of bleomycin significantly increase HP and MDA biomarker levels in compare to the sham group (P<0.001). In the positive control group treated with pirfenidone, there was mark reduction in both HP and MDA levels (P<0.001). Both hydroalcoholic and aqueous extracts of D. kotschyi significantly prevented elevation of HP and MDA levels at above oral doses in compare to the vehicle treated control group (P<0.001). Conclusion: Elevation of HP and MDA levels in the lung tissue is an indication of fibrosis and oxidative stress induction. Prevention of HP and MDA levels in this experiment indicate that D. kotschyi extract is a suitable remedy for treatment of respiratory fibrosis induced by drugs such as bleomycin. Keywords: Dracocephalum kotschyi, fibrosis, malondialdehyde, pirfenidone,
hydroxyproline.
110
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 14044
A review of the role and effects of miRNAs in the diagnosis and prevention of diabetes
Javad Yaghmoorian Khojini BSc in Cellular and Molecular Biology, Faculty of Biological Sciences and Technologies, Isfahan University, Isfahan, Iran
Introduction: Diabetes is one of the most important and well known chronic metabolic disease which its widespread prevalence in recent years has made it one of the major global health concerns. In this study, we will investigate the role and effects of miRNAs as regulators of gene expression in the diagnosis and prevention of diabetes. Description: Diabetes is one of the leading causes of morbidity and mortality worldwide. If left unchecked, it can lead to many complications, including heart attack, stroke, kidney failure, vision loss, neurological problems and an increased risk of fetal death. MiRNAs are small noncoding RNAs that contain 19-25 nucleotides and act as functional barriers to the translational stage by coupling to the 3’untranslated region of mRNAs. Circulating miRNAs due to their high stability properties can be used as biomarkers and drug targets in the control of diabetes. The mechanisms of these activities is further mediated by inhibition of miRNA activity by the effect of miRNA inhibitors, which are antisense oligonucleotides with a reverse miRNA complement sequence, or by reverting miRNA expression to the first state by MicroRNA mimics. MiRNAs can also be used as a series of sensors in diabetes, for example microRNA-375, the expression of which changes during glucose uptake. Discussion and conclusion: Failure to control or treat diabetes will cause irreversible cardiovascular, renal and neurologic complications. About diabetes, many miRNAs have been studied so far, including miR-375, miR-103, and miR-107. Extensive studies are under way to more effectively use miRNAs for therapeutic purposes of diabetes treatment and biosensors and biomarkers for early detection of diabetes.
111 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 15085
Determination of deleterious SNPs of PIK3CA gene and their impacts on the structure of PI3K
Reyhane Chamani 1, *, Parisa Naji 1 1. Department of Biology, Faculty of Science, Yazd University, Yazd, Iran *[email protected]
Introduction & Objectives: PIK3CA mutations are common activating mutations in breast cancer occurring in 20–30% of all cases. These mutations are potent predictive markers for responses to PI3K inhibitors in breast cancer. Also, they could have a negative impact on patient survival. The gene encodes the p110α catalytic subunit of PI3K. This protein plays a key role in PI3K/AKT/mTOR signaling pathway. Single nucleotide polymorphism presents in coding region of proteins (nsSNPs) has the potential to alter structure and function of the protein. Hence, it is necessary to differentiate the potential harmful nsSNPs from the neutral ones. Methods: In the present study, using NCBI dbSNP database, 26 nsSNPs were retrieved and functionally deleterious nsSNPs predicted by two different in silico tools including SIFT and Poly- Phen-2. The I-Mutant 2.0 server was used to predict the change of protein
stability due to mutations. Degree of conservation32T 32T of mutations among the populations was determined using MEGA 7 software. The Secondary structure of protein was predicted by PSIPRED. In order to further analyze the effect of the deleterious mutation on the protein structure, the complete PI3K protein structure was modelled by SWISS-MODEL and analyzed using PyMOL. Results: 6 nsSNPs including V344M, I112N, V356I, E135K, G914R, and E545V were predicted as highly deleterious and thus subjected to further analyses, such as conservation, stability, 2D and 3D structure. Results showed that these nsSNPs are highly conserved and can decrease protein stability. In addition, they change secondary structure including beta sheets and coils in mutant proteins. Hydrogen bond formation is altered due to mutations as analyzed by PyMOL. Conclusion: Our data suggest that structure and function of PI3K can be disturbed by various nsSNPs and this in silico analysis will be of great help while considering large scale gene studies and also in developing drugs. Keywords: PIK3CA; nsSNP; in silico tools; Mutation; Breast cancer
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 15236
Bioinformatics study of single nucleotide polymorphism rs1800469 and hsa- miR-22-5p related to it in Acute Lymphoblastic Leukemia
Sahar Akbari1*, Massoud Houshmand2 1. Department of Genetics, Department of Biology, High Institute nurdanesh, Meymeh, Isfahan, Iran. 2. Medical Genetic Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran. *[email protected]
Introduction & Objectives: One of the most common malignancies in Iran is leukemia. Evaluation of biomarkers of this disease is very importance. One of the genes involved in this cancer is TGFB1. Rs1800469 is located on TGFB1 and contains the A allele (dominant allele) and the G allele (SNP or allele). The aim of the present study was to investigate the association of rs1800469 with has-miR-22-5p and the role of TGFB1 in leukemia pathway. Materials & Methods: The micRo-pir was first used to identify microRNAs associated with TGFB1. Next, has-miR-22-5p was selected as one of the related microRNAs. This microRNA binds to the region of rs1800469. By confirming the role of has-miR-22-5p in leukemia through the phenomiR database, we identified the target genes from the miRWALK2.0 database and obtained the related signal pathways using the DAVID and KEGG databases. Results: According to studies, has-miR-22-5p binds with high affinity at the rs1800469. The case was that the microRNA binding energy of the SNP allele was higher and spontaneous, and was considered as the research hypothesis. In people with the hsa-miR- 22-5p binding site, the SNP allele has greater binding ability and microRNA inhibitory action. According to the information obtained from the articles, has-miR-22-5p is an oncomir. So, it will increase expression in cancer. Therefore, TGFB1 gene inhibition will be increased. Conclusion: TGFB1 is down-regulated due to its inhibition and leads to cancer pathway. Consequently, this gene is a tumor suppressor in part of the cancer pathway due to inhibition by hsa-miR-22-5p. Based on the research hypothesis, it is expected that a SNP allele will be found in the primer design of rs1800469 in the in-vitro study of individuals with leukemia. It is anticipated that the presence of the SNP allele in rs1800469 may increase the risk of cancer and can be used as a prognostic factor for leukemia. Keywords: leukemia, TGFB1, hsa-mir-22-5p, rs1800469
113 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 16265
Statistical analysis of insect anticancer peptides
Azadeh Teimury*, Zahra rezvani Department of Cell and Molecular Biology, Faculty of Chemistry, University of Kashan, Kashan, I.R. Iran
Introduction: Compared to conventional cancer treatments such as radiotherapy or chemotherapy, a way to protect healthy cells can be suggested like biological treatmentsthe such as use of proteins, peptides, etc that have been studied against cancer. Insect resistance to pathogens has certainly contributed to their proliferation and diversity and have a very strong innate immune system to annihilate aggressive pathogens, most insect antimicrobial peptides are small and show activity against bacteria or fungi as well as some parasites and viruses. Description: Based on data from The Antimicrobial Peptide Database, we have collected about 300 insecticidal antimicrobial peptides that have various activities including antifungal, antibacterial, antiviral, anti-inflammatory, antioxidant, etc. There were also 21 peptides with anticancer activity Now due to the cancer dilemma, we examined those peptides that had anticancer properties. We investigated these peptides for their hydrophobicity, chain length, and the type of amino acids used and their characteristics. Discussion and conclusion: After statistical comparison, about 3% of peptides had a negative charge and 27% had a positive charge, about 51.5% of the peptides were non- polar and aliphatic which is a large percentage, and 18% were polar uncharge. The average length of polypeptide chains was 23, net charge 4.5 and hydrophobicity 47.5%, which is a significant number. Comparison of these numbers and using their results could be useful in basic studies on these peptides and provide a basis for designing new experiments on them.
114 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 17680
Effects of intraction of Orexin B Antagonist on the Fatty Acid Synthase Enzymes Genes Expresstions of Milk Lipid in the Lactating Rats
Shima Jafari Zandieh, Homayoun Khazali
Department of Animal Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran. Introduction and objection: Milk fat plays an important role in determining the quality of dairy products. Breast epithelial cells of lactating animals are largely active in triacylglycerol synthesis. The de novo synthesis of the FAS enzyme plays an important role and is used in the elongation stage. The Orexin B neurons were located in lateral hypothalamus (LHA). Orexin B or hypocretin 2 binds to the orexin/ hypocretin2 receptor. The aim of this study was to determin the effect of the intraction of the Orexin B antagonist on the expression of FAS gene involved in milk lipid synthesis in the mammary gland. Materials and Methodes: Rats that recieved salin and the Orexin B receptor antagonist at doses of 1, 2 and 4 µg/50µL. intraductal injection througt the teat can be accessed to the breast epithelium. After anesthesia and disinfection of the breast site, 20 microlitr of the materials with the desired doses is injected into each mammary duct use a 31 gavage ansolin needle. We remove the tissue 6 hours after the injection and get the result with Real Time PCR. Gene expression analysis was performed using SPSS software. Result: The results of Real Time PCR data showed that intraductal administered Orexin B antagonist increased FAS enzyme seminal activity compared to the control group(P<0.05). Conclusion: The present study demonstrates the positive effect of successful injection of Orexin B antagonist JNJ on the FAS gene. Keywords: Orexin B, Milk, Antagonist JNJ.
115 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 18228
MicroRNAs as novel Potential biomarker in gastric cancer: Diagnostic and prognostic biomarker
Maryam Zarin1, Mohsen Soosanabadi1* 1. Department of medical Genetics, Semnan University of Medical Sciences, Semnan, Iran.
Introduction: Gastric cancer (GC) has a high incidence and ranks second in the leading causes of cancer related death. Despite many advances in treatment of GC, patients show poor prognosis and the 5-year survival rate is 5–20% and >80% of cases are diagnosed at the middle to late disease stage. Therefore, identifying novel biomarkers open new landscapes in diagnosis and prognosis for various stages of GC. Today, the existing cancer biomarkers such as MG7-Ag, CEA, CA199 and CA50 in clinical diagnosis cannot be effectively applied to the clinical diagnosis of GC because of their low sensitivity and specificity. Large part of gastric cancers causing genes regulate with microRNAs (miRNAs) that bind to 3΄ untranslated region (3΄UTR) of mRNAs. Detection of miRNAs, in tissue also in serum/plasma, may enhance the sensitivity and specificity of diagnostic and prognostic tests for early stage gastric cancer. Method: We carried out PubMed search with various combinations of these Keywords; circulating, miRNA, microRNA, prognostic biomarker and diagnostic biomarker with gastric cancer. The results included 80 articles with specific criteria that published between the periods 2005 to 2018. Result: Some of these miRNAs were significantly up-regulated in GC endothelium compared to normal endothelium such as miR-21, miR-27a, mir-34b, mir-34c, mir-128a, miR-20b and miR- 20a also some of Circulating miRNAs in serum/plasma were up-regulated are miR-20b, miR-20a, miR-17, miR-106a, miR-18a, miR-21. In the other hand, other miRNAs such as mir-128b, mir- 129 and mir-148 were reported to be down-regulated in GC tissue and miR-375, let-7a, miR-218 and miR-195-5p in serum/plasma of patients with GC. MiRNA expression profiles and next- generation sequencing results demonstrated that some miRNAs such as miR-375, miR-106a, miR- 21 and MiR-421 in tissue and miR-1, miR-20a, miR-27a, miR-34 and miR-423-5p in blood are as a diagnostic marker and the other miRNAs are correlated with prognosis, including miR-10b, miR- 21, miR-223, miR-338, let-7a, miR-30a in tissue and miR-196a, miR-200c, miR-21, miR-17- 5p/20a as a circulating miRNAs. Conclusion: Finally, miRNAs has a great potential to serve as new biomarkers in the detection and prediction of prognosis of GC. Keywords: gastric cancer, miRNA, biomarker.
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 18376
Folate supplementation's effect on epidemiology of Colorectal Cancer
Mona Shayan Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran
Introduction: It has been reported that there is approximately 40% reduction in the risk of colorectal cancer (CRC) and adenomas in individuals with the highest dietary intake of folate and vitamin B12 as compared with those with the lowest intake of these nutrients. Given that folic acid (FA) has a crucial role in gene expression that may be blocked by DNA methylation which potentially increase the rate of carcinogenesis. Description: The existence of 5-methyltetrahydrofolate (an active form of FA) is necessary for the function of the ubiquitous enzyme methionine synthesis (MS) as a methyl donor and vitamin B12 as a cofactor. When methionine amount is low, more folate is applied as methyl-tetrahydrofolate to form methionine. The possibility of low amount of methylene- tetrahydrofolate is required for DNA synthesis. Before being changed to tetrahydrofolate where it can enter to the folate pool, FA have to be altered to dihydrofolate by dihydrofolate reductase (DHFR) in the liver with one absorbance. It has been reported that 400 mcg surplus FA might saturate the DHFR enzyme, as a result it doesn't alter to folate and enter the folate pool, therefore the amount of unexpected FA that has been hypothesized as a possible pathway for carcinogenesis is considerable. Obviously, high folate consumption may be associated with diminishing CRC risk. Discussion and conclusion: Various studies have indicated that lower folate level is related to higher risk of colorectal adenomas and cancer, while some epidemiological researches have reported evidence recommending that FA supplementation and high amounts of folate in blood potentially be associated with an increased CRC incidence. More studies should be done due to clarifying whether this discrepancy.
117 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 18522
Study of the influence of raster angle on the mechanical properties of 3D printed Polycaprolactone / 45S5 Bioglass® scaffolds
Narges Ezati 1, Zohre Mousavi Nejad 2, Mojde Azizi 3,4 1. Biomedical Engineering Department, Maziar University, Noor, Mazandaran, Iran 2. Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center, Karaj, Iran 3. Department of Mining and Metallurgical Engineering, Academic Center for Education, Culture and Research, Yazd Branch, Iran 4. Academic Center for Education, Culture and Research, Yazd Branch, Iran
Introduction & Objectives: Fabrication of bone tissue engineering scaffolds due to its high sensitivity to physicochemical properties including mechanical properties has always been controversial. Since the mechanical strength of the scaffold is directly related to its geometry of pores, suitable porosity geometry is needed in the manufacture of a scaffold with mechanical properties similar to natural bone. Materials & Methods: In the present study, 3D printing was employed to fabricate Polycaprolactone (PCL) / 45S5 Bioglass® (BG) scaffolds. After 3D printing of PCL/BG with three different angles of 0°/90°, 0°/60°/120°, and 0°/45°/90°/135°, the influence of raster angle on mechanical properties, namely Ultimate Tensile Strength, Yield Strength and Modulus of Elasticity were determined. Results: It was found that the raster angle is an important parameter that significantly affected the mechanical properties of the specimens. The results showed that scaffolds with raster angles of 0°/90° presented the highest compressive strength and modulus of elasticity. Conclusion: This study evaluated the effect of additive manufactured scaffold geometry on mechanical properties. It focused on the orientation of the printed strands. The results of this study demonstrate how we can control the mechanical properties of 3D printed scaffolds by changing the raster angle.
Keywords: 3D Bioprinting, Bone tissue engineering, Polycaprolactone / 45S5 Bioglass® , Raster angle
118 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 18801
in silico study of association of rs35613052 with hsa-miR-770-5p in acute leukemia
Nioosha Ataee1. *, Niloofar Ataee2*, Massoud Houshmand3. *, Nasrin Fayahi4, Atefeh Zamani5
1. Biotechnology Division, Department of Biology, Shahi Ashrafi Esfahani Nonprofit University, Isfahan, Iran ([email protected]) 2. Islamic Azad University of Falavarjan, Isfahan, Iran ([email protected]) 3. Medical Genetic Department, National Institute for Genetic Engineering and Biotechnology ([email protected]) 4. Gene RAZ BUALI, Genetic and Biotechnology Academy, Isfahan, Iran ([email protected]) 5. Gene RAZ BUALI, Genetic and Biotechnology Academy, Isfahan, Iran ([email protected])
Abstract: FLT3 overexpression is a recurrent event in various acute leukaemia subtypes. This transcriptional deregulation is important to define the prognostic risk for many patients. Collectively, the results of previous studies showed that the overexpression of FLT3 is a potential risk factor in leukemia. In this study, we predicted by use of mirbase, mirtarbase, mirwalk, ncbi, david, mirnasnp and genemania databases. In a study of the KeGG signaling pathway and pathways in cancer, we found FLT3 gene that led to cancer. we predict hsa- miR-770-5 targets A allele in rs35613052 of FLT3gene more than G allele. Therefore, it is expected With The presence of G allele in rs35613052, hsa-miR-770-5p have less binding and inhibitory effect. Therefore, gene expression in these individuals with the G allele is predicted to increase and we expect the G allele to be more common in sick people. Therefore, given the oncogenic role of the gene and the kegg pathway, it is expected to be less common frequent in patients with the A allele. Keywords: FLT3, rs35613052, hsa-miR-770-5p, AML, ALL
119 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 19584
Mesenchymal stem cells as a therapeutic method for COVID-19
1 2 3 4 Ayda Pourmostafa, Reza Samanipour, AmirHossein Tavakoli, Adel Marzban 1. Department of research and development Iranian Tissue Product Co, Tehran, Iran. 2. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 3. Iranian Tissue Bank & Research Center, Tehran University of Medical Sciences, Tehran, Iran. 4. Department of research and development Iranian Tissue Product Co, Tehran, Iran. Email address:[email protected]
Introduction: severe acute respiratory syndrome coronavirus 2 (COVID-19) grown to be a worldwide pandemic. It has infected more than 14milion people around the world and caused approximately 600,000 deaths. Almost 14% of patients present with Acute Lung Injury (ALI) and acute respiratory distress syndrome (ARDS). As no specific therapeutics is introduced as a certain treatment, the immune system and inflammation are proposed to play a central role in COVID-19 pathogenesis. There are several completed, ongoing studies which can be served as evidence that MSCs can be treated without the occurrence of severe adverse events. Description: COVID-19 induces excessive host immune responses that are always accompanied by cytokine storms (CS) and subsequent ALI or even ARDS, resulting in multiple organ dysfunction. Mesenchymal stem cells (MSCs) have been shown to possess a comprehensive, powerful immunomodulatory function. MSCs produce extracellular vehicles (EVs) that can help ameliorate acute lung injury by inducing anti-inflammatory macrophages, adjusting the level of B-cells, and regulatory dendritic cells, and can inactivate T cells. Regards to current researches for moderate to severe ARDS induced by H7N9 and H1N1 influenza, infusion of MSCs caused no serious adverse effect or allergic reaction, and numbers of patients who recovered increased in comparison to the negative control groups. There are 49 MSCs globally trials, explicitly targeting COVID-19 registered on the NIH Clinical Trial website as of 18 Jul. Discussion and conclusion: To sum up, published investigations and reported studies of the human MSC therapy has emerged as a promising treatment method for COVID-19. Meanwhile, the research overview should be designed based on further experiments to understand the underlying mechanism of action of COVID-19 in more detail and accordingly optimize MSC therapy, including parameters such as culture conditions. Keywords: Mesenchymal stem cells, COVID-19, Clinical Trial
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 20035
A study of probability distribution of the single nucleotide variants of rs7579 and rs34713741 inSEPP1 and SELs genes of the colorectal cancer patients compared with a control group of random patients in Isfahan AL-zahra hospital
* Guilda Amini 1 , Rasoul Salehi 2 1. Depatment of Genetic and Molecular Biology, Medical School Isfahan, Iran 2. Department of Biochemistry, Falavarjan Islamic Azad University, Falavarjan, Iran
Introduction: Colorectal cancer is one of the most common cancers worldwide, and according to WHO in 2013, 45 of every 100000 people were develop this cancer. In studying genetic factors that cause this cancer, attention is focused on mutations. Nowadays, extensive and numerous studies are conducted on the relationship between genetic changes such as polymorphisms and the risk for developing various kinds of cancer. Research has shown that some of selenoproteins polymorphisms are also involved in the development of various types of cancer, among which the pivotal role of rs7579 is in the SEPPI and rs34713741 in the SELS genes.
Methods: In this research, peripheral blood samples were randomly taken from 60 colorectal cancer patients and 74 healthy people who were matched to the patients with respect to age and gender. The DNA genomes were extracted from the blood samples using kit, and the HRM technique was employed to study two polymorphisms.
Results: HRM diagrams related to the polymorphism were analyzed, the genotypes in the case and control groups were then compared, and a significant relationship was observed between frequency distributions A, G alleles of the rs7579 polymorphism (p value = 0.03). In the SELS gene, no significant correlation was found between allele frequency and genotypic distribution in rs34713741 polymorphism and colorectal cancer. (pvalue=0.93).
conclusion: Based on results of this study, we can suggest that there is a significant relationship between the rs7579 polymorphism of the SEPPI gene and increased risk for colorectal cancer development. In this study, there was not found any significant relationship between rs34713741 in the SELS gene and colorectal cancer. Keywords: Colorectal cancer, SEPPI gene, SELS gene, HRM, polymorphism
121
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 20141
Bioinformatics study of single nucleotide polymorphism rs2070672 and its association hsa-mir-199a-5p in patients with acute lymphoblastic leukemia
Toktam sheidaeian1*, Massoud Houshmand2 1. Department of Genetics, Department of Biology, High Institute nurdanesh, Meymeh, Isfahan, Iran. 2. Medical Genetic Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran. *[email protected]
Introduction & Objectives: Acute lymphoblastic leukemia accounts for 80% of childhood leukemia. CYP2E1 is one of the important genes involved in this cancer. Rs2070672 is located on the CYP2E1 gene and contains the A allele (dominant allele) and the G allele (SNP allele). The aim of this study was to evaluate the role of CYP2E1 and its association with hsa-mir-199a-5p in the pathway of acute lymphoblastic leukemia. Materials & Methods: At first, a microPIR database was used to identify microRNAs associated with CYP2E1. After investigating the role of microRNAs in leukemia cancer through the phenomiR database and articles, finally, hsa-mir-199a-5p was selected as the microRNA associated with this gene and acute lymphoblastic leukemia. This microRNA binds to the CYP2E1 transcript and the region that contains the rs2070672 mononucleotide polymorphism. The target genes hsa-mir-199a-5p were obtained from the miRWALK2.0 database. The associated signal pathways were identified using the DAVID and KEGG databases. Results: According to information obtained from the microPIR database, while the rs2070672 allele is SNP, the hsa-mir-199a-5p is more possibility bind to the gene. According to phenomiR data, hsa-mir-199a-5p is overexpressed in acute lymphoblastic leukemia cancer. Due to the increased expression of the microRNA inhibitory role, gene expression is reduced in this state. Conclusion: In the case of the SNP allele, expression of CYP2E1 decreased due to being further inhibited by hsa-mir-199a-5p and leads to cancer progression. As a result, this gene is a tumor suppressor. It is expected that by designing primer at rs2279744, the SNP allele will be in leukemia patients with practical design. It is predicted that the presence of the SNP allele in the CYP2E1 gene may increase the risk of cancer and can be used as a prognostic factor for acute lymphoblastic leukemia. Keywords: leukemia, CYP2E1, hsa-mir-199a-5p, rs2070672
122 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 21367
Novel therapeutic approaches against metastatic melanoma cells
Mahsa Nikbakht1, Fatemeh B. Rassouli1,2* 1. Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran 2. Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran Corresponding author’s e-mail: [email protected]
Introduction: Melanoma, the malignancy of melanocytes, is a serious disease with growing incidence in comparison with almost any other cancer. Despite recent improvements in prevention, diagnosis and treatment of melanoma, the survival rate of patients is low, mainly due to metastasis of cells beyond their primary site. Resection of the lesion, alone or along with chemotherapy and radiotherapy are conventional treatments for metastatic melanoma. Since chemical agents and radiation cause serious side effects, and their efficiency depends on the severity of disease at the time of diagnosis, studies have aimed to introduce novel therapeutic approaches against melanoma. Description: In search for recent advances in melanoma treatment, published articles including Keywords metastatic melanoma, nanomedicine, immunotherapy and photodynamic therapy were extracted from databases Web of Science, PubMed and Scopus. Discussion and conclusion: The critical role of nanotechnology in improvement of melanoma drug delivery is not doubtable, since nanostructures act as carriers that promote the cellular uptake of anticancer drugs and also affects their stability, permeability, and hydrophilic adaptations. Interestingly, incorporation of nanoparticles to antibodies promotes drug attachment to malanoma cells, and reduces the overall toxicity in adjacent non-cancerous cells. The other approach is molecular-targeted therapy by which CTLA-4 antibody is used to improve the cancer-fighting ability of innate immune system. However, efficacy of this method is influenced by its immune-related side effects and developed resistance. Last but not least, photodynamic therapy is a new modality involving a particular wavelength of light and a photosensitizing agent, which produces reactive oxygen species to achieve photocytotoxic effects. Beside its less invasiveness, this approach has limited side effects since it induced localized apoptosis, autophagy, and/or necrosis in cancerous tissue. In novel methods, nanoparticles are conjugated to photosynthetic drugs to improve drug delivery, such as conjugation of doxil, a FDA approved drug, to pegylated liposomal nanocarriers. Keywords: Metastatic melanoma, Nanomedicine, Photodynamic therapy.
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 21501
Role of Magnesium in Cancer Treatment
Authors: Aida Heidari1, Samane Mohamadzade1 1. Poursina Hakim Digestive Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, Iran Introduction: Cancer is a multifactorial condition results from interaction of genetic and environmental factors that modulate tumorigenesis and disease progression. Magnesium, the second most abundant intracellular cation, is involved in many biochemical reactions and modulate fundamental cell functions including proliferation, differentiation, migration and apoptosis. Magnesium deficiency has identified as a risk factor for some types of human cancers. DNA Damage and Cancer: Magnesium and has an important role in genetic stability and DNA synthesis and moderate Mg deficiency causes genetic instability, activation of cell-cycle arrest proteins, and mitochondrial DNA damage. Magnesium supports multiple DNA-repair processes so that all downstream activities of major base excision repair proteins require this element. Magnesium and tumor growth: Cell-cycle progression depends on magnesium supply and low Mg availability inhibits that. Proliferating cells contain more magnesium than resting ones. In the other words, without an adequate Mg supply, no proliferation can occur. Neoangiogenesis and Metastasization: Neo-angiogenesis is necessary for tumor growth and progression. Magnesium can affect various steps of the highly complex angiogenic process and its deficiency prevents both proliferation and migration of endothelial cells. Moreover, hypomagnesaemia may induce pro-inflammatory condition that might create a favorable microenvironment for tumor growth and progression. Magnesium transporters in cancer: The transient receptor potential melastatin 7 (TRPM 7) channels are essential for the Mg homeostasis in normal and tumor cells. Because of their versatile properties, TRPM7 channels could affect the behavior of tumor cell by modulating both proliferation and plasticity/motility. TRPM 7 expression and Mg homeostasis are altered in drug resistant cancer cells, so there might be a Mg-regulating mechanism in cancer drug resistance.
Conclusions and future perspectives: More studies should be undertaken to determine whether intervention for Mg optimization might be helpful in cancer prevention and treatment. Moreover, Mg transporter expression and/or activity may constitute novel therapeutic targets for circumvention of chemoresistance. Keywords: Magnesium, Cancer, TRPM7, Angiogenesis, Metastasis
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 22105
miRNA-mRNA Regulatory Networks of Some Neurotransmitter Receptors in Nervous System Disease
Banafsheh Ashrafnia Bioinformatic Academy of Zist Pardazesh Institute, Esfahan, Iran
Introduction and Objectives: MicroRNAs are small noncoding RNAs which modulate protein expression and play key roles in the pathways regulating neurogenesis and synaptic plasticity. These small RNAs may be important for the pathophysiology of mental disorders and may affect the efficacy of psychotropic drugs. In this project, we got microRNAs that inhibit some receptors of neurotransmitters and constructed interaction networks of miRNA-mRNA. Materials and Methods: Mirwalk database was applied to get the validated microRNAs which regulate some receptors of neurotransmitters expression (HTR1A, HTR1B, HTR1D, HTR2C, HTR3A, HTR7, GABRB3, GABBR2, ADRA1B, ADRA2B, ADRB3, UBE2D2, GRIN2B, GRIA2, GRIK2, GRM1, GRM3, GRM4, GRM5, GRM6). After that, these data were imported in cytoscape software, and miRNA-mRNA networks were constructed and analyzed. Also, HMDD database was applied to evaluation of relationship between significant miRNAs in the network. Results: Evaluation of miRNA-mRNA interaction networks of neurotransmitters receptors showed that these interaction networks include 253 nodes. MiR-124-3p (degree:7) and miR-335-۵p (degree:5) have the most degree in the network. Also, HMDD (the Human microRNA Disease Database version3.2) indicated that expression of miR-124-3p is changed in some Nervous System Diseases such as Depression Disorder, Bipolar Disorder, Schizophrenia, Neurodegenerative Diseases, Alzheimer’s Disease, and Neuroinflammation. Conclusion: Results indicated miR-124-3p is significantly powerful miRNA that regulated series of important receptors genes in the brain. Therefore, miR-124-3p could be considered a perfect target for the treatment of nervous system disease. Keywords: Interaction network, microRNA, neurotransmitter
125 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 22256
Co-incidence of ductal breast cancer and gastrointestinal cancers: A case- series study
Erfan Khorram1, Mehrdad Zeinalian1* 1. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract: Hereditary cancer syndromes are defined as genetic disorders that increase the risk of developing certain types of cancers. The increased risk of breast cancer (BC) is well- established in association with the majority types of these syndromes. Previous studies have reported the co-incidence of gastrointestinal cancers accompanied by BC in various types of hereditary cancer syndromes like hereditary breast and ovarian cancer syndrome (HBOC), Peutz-Jeghers syndrome (PJS) and Cowden syndrome (CS). Although clinical data have confirmed the simultaneous occurrence of lobular BC and gastrointestinal cancers in some families, to the best of our knowledge there is no report on the co-incidence of ductal BC and gastrointestinal tumors in inherited syndromes. In the present study, we report BC in patients who were affected by ductal ones from two large pedigrees in which gastrointestinal malignancies have been reported previously. Our observation of these two uncommon phenotypes at the same time recommends refining clinical guidelines that are merely based on phenotypes.
126 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 22350
The Effect Anticancer Characteristics of the Alcoholic Extract of Dracocephalum kotschyi in the Breast Cancer Cell Line
Farinaz Khosravian1,2,3*, Afrooz Golestanian1,2, Nasrin Hadi4, Faezeh Namazi4, Mirza Mohammad Raisinia5, Mansoor Salehi1, 2,3 1. Cellular, Molecular and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran. 2. Medical Genetics Research Center of Genome, Isfahan University of Medical Sciences, Isfahan, Iran. 3. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. 4. Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran. 5. Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran.
Introduction and Objectives: Breast cancer, the most frequently occurring cancer among women, is known as the fifth cause of death in Iran. Dracocephalum Kotschyi, which belongs to the Labiatae family, has been used in Iranian medicine for many years. There are almost one hundred different compounds in this plant namely as Terpenoids, Flavonoids, and Alkaloids which are considered as antioxidants substances. In this study, we investigated the effect of Dracocephalum Katschyi alcoholic extract on BAX genes expression level and proliferation on the SKBR3 cell line in breast cancer. Materials and Methods: From the Dracocephalum leaves and aerial parts, one hundred grams of powder was produced and was solved in 250ml ethanol. Using the MTT-method, different sample concentrations of the 10, 50, 100, and 500µg/ml were prepared. The effects of an alcoholic agent on cultured SKBR3 cell lines and BCL2 expression were investigated. RNAs were extracted using a QuantiTed Reverse TranscriptionKit and cDNA was synthesized. Next, through the use of a quantitative real-time polymerase chain reaction, the BAX expression was evaluated. Results: The experimental research on the Dracocephalum Kotschyi on cell proliferation and cell viability indicated that this plant is an anti-cancer substance. Besides, the IC50 value of SKBR3 cells in 24hrs was 102.1. Bax genes expression increased exposing this solvent and this was observed in the concentration of 500µg/ml. Conclusions: In conclusion, the promising results show that Dracocephalum Katschyi alcoholic extract can be used for producing anticancer medicines for breast cancer treatment in the future. Key Words: BAX, Breast Cancer, Dracocephalum Kotschyi .
127 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 23077
Recent strategies for noninvasive glucose monitoring
Rahil Mosalla nejad1, Seyyed Ebrahim Moosavi fard2* 1- Student Research Committee, Jahrom University of Medical Sciences, Jahrom, Iran 2- Department of Advanced Medical Sciences & Technologies, School of Medicine, Jahrom University of Medical Sciences (JUMS), Jahrom, Iran
Abstract: Diabetes can be defined as a complicated and worldwide epidemic metabolic disorder disease. As estimated by the World Health Organization by year 2030,366 million people will have diabetes mellitus. Blood glucose concentration is a key indicator of health of diabetic patients, so glucose is considered as a biomarker according to the definition of National Institutes of Health. Methods for measuring glucose are divided into invasive (Finger-pricking and Implantable) and non-invasive (Tissues and Fluids). Because of several disadvantages like risk of infection and damage, wearable and mobile technologies in non-invasive glucose monitoring have attracted much attention. Herein, we review recent advances in bio-fluids and tissues based non-invasive glucose measurements including new materials, concepts, and engineering techniques. Bio-fluids, such as sweat, saliva, interstitial fluid (ISF) and tears, reflecting the physiological conditions in the core body, are very attractive due to having the most sampling sites outside the body, an ease of collection, device placement and comfort. But, the major challenges for accurate fluids glucose measurements are stemming from fluctuations in environmental parameters (i.e. temperature and pH variation), low production rate, and the mixing of old sample with new fluids. Tissues are flexible, aesthetically pleasing and can be easily mated with the human skin. But, their disadvantages are skin irritation caused by the RI process, the long necessary warm up time and the need for calibration. Tissues include glucowatch (enzymatic electrochemical glucose sensor), sugar beta (a disposable adhesive skin-patch), temporary tattoo (screen-printing electrode platform on skin) and earlobe clip (using ultrasonic, electromagnetic and thermal technologies). The development of electrochemical non-invasive glucose monitoring for diabetes management is just beginning and extensive efforts are required to address its key technological challenges.
128 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 23255
In silico molecular docking of IDH1 r132 mutant inhibitors as anti-cancer agents: a comparative study
Rahman Emamzadeh1*, Elham Aghaei1, Mahboobeh Nazari2 1. Department of cell and molecular biology and Microbiology, Faculty of Biological Sciences and Tecnologies, University of Isfahan, Isfahan, Iran*. [email protected] 2. Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Abstract: Isocitrate dehydrogenases (IDHs) are enzymes that catalyze the oxidative decarboxylation of isocitrate, producing α-ketoglutarate (αKG) and CO2. In humans, IDHs exist in three isoforms: IDH1, IDH2 and IDH3. IDH1 and IDH2 use nicotinamide adenine dinucleotide phosphate (NADP+) as a co-factor and function as homodimers. IDH1 is mutated in various types of human cancer such as adult acute myeloid leukemia (AML) and glioma , and the presence of this mutation is associated with improved responses to irradiation and chemotherapy in solid tumor cells. Almost all of the IDH1 mutations occur at codon R132. Its inhibition can suppress or even stop the cancer cells proliferation. Several IDH1 R132 mutant inhibitors have been designed to date. Comparing binding energies of the inhibitors provides a relatively good insight of what is the most effective structure and where is the best region of action. molecular docking was performed by using AutoDockTools-1.5.6 on the inhibitors including AG-881(vorasidenib),BAY-1436032, GSK-321, IDH-305, IDH-889, ML-309, AG-120(Ivosidenib) and AGI-5198. The binding energies of the inhibitors were -2.63, -7.95, -10.34, -5.49, -7.18, -3.17, -2.55 and -3.36, respectively. Inhibitors that attach to the allosteric pocket (AG-881(vorasidenib), BAY- 1436032, GSK-321, IDH-305, IDH-889) generally have better binding energies than those which bind to the active site (ML-309, AG-120(Ivosidenib), AG-5198). Also, among these inhibitors, GSK-321 was the best inhibitor in term of binding energy. Result may be used to design and develop anti- IDH1 inhibitors in the future. Keywords: Isocitrate dehydrogenase (IDH), Molecular docking, R132 Mutation, Inhibition, Binding energy
129 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 23637
Early-phase diagnosis of plasma miRNA-21 in cancerous patients based on time and intensity of color changing in gold nanoparticles
Asma Hamidi1, Hamidreza Mollasalehi2* 1. Protein Research Center, Shahid Beheshti University, G.C, Tehran, Iran 2. Department of Cellular and Molecular Biology, Faculty of Biological Sciences and Technology, Shahid Beheshti University, G.C, Tehran, Iran. Postal Code: 1983969411 * [email protected]
Introduction & Objectives: The expression of some miRNAs is increased in the blood of cancerous patients. Therefore, the early detection of cancer by measuring the concentration of miRNAs helps to improve treatment of these cancers. Evaluating the concentration of miRNA-21 by gold nanoparticles is a molecular method for early-phase diagnosis of cancer. Materials & Methods: MiRNAs from the plasma of 30 patients with breast, colon, sarcoma, and bladder of cancers were extracted. Subsequently, the extracted samples were subjected to specific Au-NProbes aggregation. Finally, the time and intensity of color changing were measured using spectrophotometry. Results: Color-changing of Au-Nprobe-21 from red to purple-gray was observed in clinical specimens of sarcoma, breast, colon, and bladder. Average intensity of color changing of Au- Nprobe-21 tested for bladder and sarcoma samples was reported 0.129 and 0.127 respectively. The average intensity of colon samples and breast samples was 0.165 and 0.14, respectively. Besides, the average time of aggregation of gold nanoparticles tested for bladder and sarcoma samples was 3.5 s and 3.5 s respectively. The average time of colon samples and breast samples was 3.8 s and 4.1 s, respectively. Conclusion: In conclusion, quantitative investigation of miRNA-21 concentrations by aggregation and color-changing could facilitate development of a point-of-care biosensor for rapid and simple prognosis and diagnosis of common lethal cancers. Keywords:Early detection, miRNA-21, gold nanoparticles, color changing, quantitative analysis.
130 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 23657
Metabolomics in cancer biomarker discovery; new trend in personalized medicine for breast cancer
Maryam Zarin1, Mohsen Soosanabadi1*
1. Department of medical Genetics, Semnan University of Medical Sciences, Semnan, Iran. 2. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Corresponding author: Mohsen Soosanabadi
Introduction: Breast cancer has become the most frequent malignancy, during past decades. The burden of breast cancer will soon become a challenge to the health economy in Iran, concluding the needs for essential works in this era. Many individuals will die from progressive, advanced breast cancer. These facts reveal that current diagnostic tests including assessment of features of the tumor, e.g. size, grade and analysis of ER/PR, HER2 expression, TAG72.4 and CA549 by common methods (i.e. FISH, IHC and ELISA) isn’t sufficient and individuals with similar cancer phenotypes display marked diversity in outcome and response to intervention. Description: BC development is associated with fundamental metabolic disturbances. Nowadays metabolomics appeal as a valuable, complementary clinical tool. Elucidation of these metabolites, available in bio fluids (i.e. Serum, urine and saliva) allowing recognition of new biomarkers for diagnosis and assess the risk and stratification, new therapy targets and novel approaches for monitoring response. Metabolomics studies metabolic responses to pathophysiological or genetic factors. Metabolic biomarkers as reliable tools, reproducibly detected in samples and available in samples that collected with minimal invasion to patient. There are many qualitative and quantitative laboratory analyses (i.e. gas and liquid chromatography, mass spectrometry, capillary electrophoresis and nuclear magnetic resonance or NMR) to identify metabolic potential biomarkers. Multiple studies demonstrated the application of metabolomics in the risk factors, diagnosis, treatment, prognosis and determining the degree of malignancy based on metabolite patterns. Discussion and conclusion: Finally, the metabolomics approaches are not only useful for diagnostic tools and patient stratification, but may be mapped on metabolic network to reflect disease states. These biomarkers may provide clinicians with more accurate personalized treatments. In this review, we summarized some example for application of metabolomics in each area of breast cancer management. Keyword: Breast cancer, Metabolomics, Biomarker, Personalized Medicine.
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 23838
Comparative evaluation of 3D polycaprolactone scaffolds fabricated by 3D printing and salt leaching methods
Ali Honarvara, Ali Valianid, Batool Hashemibenia, sho,leh ghaedaminia, Saeed Karbasib, Department of Anatomical Sciences, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran a Department of Biomaterials, Tissue Engineering and Nanotechnology, School of Advanced Medical Technologies, Isfahan University of Medical Sciences, Isfahan, Iran b. Department of Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran c
Introduction: Designing a three-dimensional ideal scaffold has been one of the main goals in tissue engineering, and various mechanical techniques have been applied to fabricate scaffolds used for tissue regeneration. Scaffolds should be biodegradable and biocompatible, provide temporary support for cell growth to allow cell adhesion, and consist of a defined structure that can be formed into customized shapes by a computer- aided design system. The formation of new tissue is affected by the porosity, pore size, and 3-D structure of the scaffold; a large pore structure is formed to deliver adequate number of cells, and the interconnected pore allows for the easy diffusion of nutrients. Biomaterials and methods of scaffold fabrication have a crucial role in tissue engineering. New 3D Printing technology has the potential to solve these problems. Materials and Methods: In this study, PCL scaffold was designed and printed with interconnecting pores by using the Bioplotter devices as novel method and compared with PCL scaffold that fabricated by salt leaching as a traditional method of scaffold fabrication. MTT assay, water absorption, scanning electron microscopy and dynamic mechanical analysis were used to characterize the mechanical properties of the resulting scaffolds. Results: The water absorption of the 3D printed scaffold was higher than those of the conventional salt-leaching scaffolds. The 3D printed scaffold presented a high compressive modulus and cell viability that was suitable for cell ingrowth compare with salt-leached scaffolds. The porosities of the conventional salt-leaching and 3D printed scaffolds were 72.43±1.75% and 59.35±0.55% respectively. Conclusions: The 3D printed polycaprolactone scaffolds had the obvious advantage that their mechanical properties compared to the salt-leached scaffolds. The scaffolds fabricated by the 3-D printed method were formed to induce cell penetration for cell ingrowth. The 3D printed scaffold was more suitable for cell ingrowth than a salt-leached scaffold. Keywords: polycaprolactone, 3D printing, salt-leaching, scaffold, tissue engineering
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 24461
Risk of Infertility and celiac disease; a case report and literature review
Khosravi Sh.¹ʼ², Yousefian E.¹ʼ³,78T 78T Bagheri N.¹, Eshaghi Sh.¹ 1. Majesty of Maryam Infertility Center, Martyr Beheshti Hospital, Isfahan University of Medical Science, Isfahan, Iran. 2. Department of Genetics and Molecular Biology, Isfahan University of Medical Science, Isfahan, Iran 3. Department of obstetrics and Gynecology, Infertility center, Beheshti University Hospital, Isfahan University of Medical Science, Isfahan, Iran.
Background: Celiac disease as an immune-mediated disorder that is commonly associated with intestinal symptoms due to genetically-susceptible to gluten, can also impact the reproductive system, resulting in increased risk of infertility, impairing embryo implantation, repeated miscarriages, premature deliveries. Material and Method: We searched PubMed for English-language articles published between 2000 and 2020, containing information about the risk of infertility and celiac disease, and studies that evaluating the prevalence of undiagnosed celiac disease in subjects with infertility. Result: According to prospective serologically-based studies, over 4% of females with reproductive disorders or pregnancy difficulties have celiac disease without any manifestations, or ultimately, fatigue associated with iron-deficiency anemia. Subsequently, amenorrhea, delayed onset of menarche, early menopause and reduced fertility in females may possibly be the primary clinical feature that finally results in a diagnosis of celiac disease. Case: The case is a 28 years old lady who had referred to a gynecologist department because of infertility for 3 years. Diagnosis of celiac disease and administration of a strict gluten-free diet for this patient result in pregnancy. Conclusion: Serological screening for suspicious cases with few or no symptoms such as unexplained infertility seems to be a cost effective method. As in females with infertility and untreated celiac disease, gluten-free diet solely, can lead to successful pregnancy. Keywords: Infertility, Celiac disease, Gluten-free diet, case report
133 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 24556
Valuable methods to identify therapeutic target pathways in cancers: CRISPR screening and RNA seq
Sorayya Ghasemi1 1. PhD of medical genetics, Assistant Professor of Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
Introduction: Cancers are one of the most hard-to-treat diseases and are highly complex with respect to molecular signaling pathways. Cancers are genetic heterogeneous diseases, and therefore the identification and understanding of the key signaling pathways responsible for the malignant phenotype of cancers will yield new potential therapeutic targets. The use of high-throughput techniques is a shortcut to quickly find the molecular therapeutic targets. Two techniques that help to identify important molecular targets are CRISPR screening and RNAseq. Description: The CRISPR screening makes it possible to modify a large number of genes and to investigate their function in a single test. Therefore, this technique can be employed for knockout of the individual genes in genome-scale functional screens such as cancerous cell functional changes. RNAseq also demonstrates the existence and quantity of RNA in a biological sample in a specific point of time, and the transcriptome changes can be detected after analysis and comparison with the normal cases. Conclusion and Discussion: Accordingly, CRISPR screening, followed by genome NGS (to figure out the characteristics of the random mutations produced by CRISPR in the genome) and RNAseq, would be performed to identify the genome and transcriptome changes in isolated cultured cells with the desired phenotypic modifications. After detection of the effective pathways and genes, main targets interactions in such data possibly determine the best gene(s) for more fruitful drug development research. Also, the respective datasets can be employed to study interactions among important genes to assist gene therapy. Keywords: Cancer, CRISPR screening, RNAseq
134 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 24592
microRNAs roles on apoptosis in pancreatic cancer
Arezoo Mosharraf 1, Zeinab Shirvani-Farsani 1 1. Department of Cellular and Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University G.C., Tehran, IR Iran. Abstract: Pancreatic cancer (PC) is a disease with a prognosis only of 5-6% of patients. Improving diagnosis, and treatment of PC need a better understanding of the molecular mechanisms and the different pathways implicated in the pathogenesis of PC. In PC, the PI3K/AKT pathway is disrupted. This pathway induces apoptosis, so that disruption causes cancer cell survival and disease progression. Several microRNAs are involved in this pathway, some of which are tumor suppressors and some are oncogenes, As discussed below. miR21 and miR221 decrease the expression of PTEN, NOTCH1, and NOTCH2 in PC and have oncogenic functions. They regulate cell growth by negative regulation of PI3K/AKT pathway. miR221 in normal tissue increase P27, P57, and PUMA expression which are tumor suppressors and inhibit cell proliferation. The expression of miR34 is suppressed in PC and decreases the expression of AKT and Bcl2. miR217 naturally reduces K-RAS expression and inhibits AKT expression, which inhibits cell survival. actually, K-RAS mutations are found in 90% of PC patients in metastatic stage, which results in cell viability and anti-apoptotic activity. Besides, miR217 expression is reduced in PC and as a result, cell growth is going out of control. Another miRNA-mediated therapy is miRNA antagonists. Single-stranded antisense designed for a particular miRNA and binds to it with high stability. These antagonists inhibit the activity of miRs or interferes with miR biogenesis. The assessment of miRNAs involved in PI3K/AKT pathway is imperative as diagnostic biomarkers and therapeutic targets in patients with PC. However, so far, only a few miRNAs have been studied at the functional or molecular levels in PC patients. Further investigations should be performed to identify, characterize and clarify the significant functions of miRNAs involved in PI3K/AKT pathway in the pathology of PC at a molecular level and use them as clinical biomarkers and therapeutic targets. Keywords: pancreatic cancer, microRNAs, PI3K/AKT pathway, therapeutic targets
135 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 24993
Early diagnosis of breast cancer using tumor-derived exosomes
Mohammad Ghasemi1, Leyla Norouzi-Barough2, Sadegh Shirian1*
Departmen of Pathology, School of Veterinary Medicine, shahrekord University, Shahrekord, Iran Department of Genetics and Molecular Biology, school of Medicine, Isfahan University of Medical Sceinces, Isfahan, Iran
Introduction: Breast cancer (BC) is the most frequently-diagnosed life-threatening cancer among women. Despite significant advances in early detection, it remains the second- leading cause of cancer-related deaths among women worldwide. The poor prognosis of most breast cancer patients is mainly due to late diagnosis. Therefore, identification of new biomarkers for early diagnosis in cancer is urgently required. Exosomes are membrane- bound vesicles, 30–100 nm in size, released from various types of mammalian cells upon the fusion of multivesicular bodies with plasma membranes. Emerging evidence shows that exosomes are involved in the development and progression of different tumor types, including breast cancer. Therefore, identification of BC-related exosomes and their underlying mechanisms in progression of BC would be helpful in the early diagnosis of BC and stablishing appropriate therapeutic strategies. Description: to highlight the potential roles of exosomes in tumor initiation, proliferation, migration, invasion, metastasis, and their potential applications in early diagnosis of breast cancer. Discussion and conclusion: Tumor-derived(TD) exosomes exist extensively in biological fluids such as blood, plasma, cerebrospinal fluid, and urine. It has been proved that (TD) exosomes are involved in constructing of a tumor microenvironment that supports the spread of a tumor, invasion and pre-metastatic niche development. In fact, exosomes by restricting of immune control and secretion of growth factors, angiopoietin factors, and cytokines from stromal cells, promote cell migration, cancer progression, and metastasis. In addition, (TD) exosomes are enriched in some specific miRNAs and proteins compared with non-tumorigenic cells and it has been well evidenced that these components play a critical role in the development of BC tumors. Altogether, it seems that breast tumor- derived exosomes can be served as worthwhile, non-invasive diagnostic and predictive biomarkers for early detection of breast cancer
136 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 25121
The study of clinical findings and chronological age in Arab horses in association with telomerase expression
Mahsa Golshani Nasaba, Fereydon Rezazadehb, Behzad Baradaranc a Doctor of Veterinary Medicine Graduate, Faculty of Veterinary Medicine, University of Tabriz, Tabriz- Iran b Associate Professor in Large Animal Internal Medicine, Department of Clinical Sciences, Faculty of Veterinary Medicine,32T
University32T of Tabriz,32T Tabriz- Iran c Associate Professor of Immunology, Immunology Research Center, Faculty of Medicine, Tabriz University of Medical Sciences
Abstract: Aging is a complex phenomenon that induces many physiological changes from tissues and organs to cells and subcellular organelles. One of the most known outcomes of aging is a decline in the responsiveness of the immune system referred to as immunosenescence. Over the past years, the average lifespan of humans and animals has extended due to improvements in science. Currently, the number of aged horses facing age-related diseases is rising drastically. Telomere is indicative of biological age and the level of telomerase activity can be a determinant of telomere length in aging cells. In the absence of telomerase, telomere length will be gradually shortened with each cell division. P53, a tumor suppressor protein, is one of the key proteins in DNA damage response that harmonizes multiple cellular functions, including DNA repair, apoptosis, and senescence. This study investigates the role of telomerase gene (TERT) and P53 gene (Tp53), in aged equids and explores the association between them and clinical manifestation of the age-related disease (Colic, Osteoarthritis, Dental disorders, PPID, and Tendonitis). Blood was collected from 30 Arab horses from around Tabriz, Iran, and categorized in three age groups (1-10, 10- 15, and >15 years old). DNA was extracted from peripheral blood; telomerase and p53 expression were measured using RT-PCR and the results were analyzed by GraphPad Prism. The analysis showed that age was directly correlated with p53 expression (P=0.001) while inversely correlated with TERT expression (P=0.0001). The overall study results suggest that manifestation of age-related diseases such as osteoarthritis and tendonitis were higher in aged horses based on a comparison among three age groups. In conclusion, the association between age and telomerase expression is analogous to previous studies in humans and other animal models. However, some studies concerning P53 function in the context of aging are controversial with present research. Keywords: Aging, Horse, Immunosenescence, P53, Telomerase
137 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 25885
Association between Toll-Like Receptor 4 and Response to Chemotherapy drugs in Breast Cancer
Nasim Dana1*, Shaghayegh Haghjooy Javanmard1, Golnaz Vaseghi 2, 1 1. Applied Physiology Research Center and Department of Physiology, Cardiovascular Research Institute, Isfahan University of Medical sciences, Isfahan, Iran. 2. Isfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical sciences, Isfahan, Iran
Introduction: Breast cancer is the most common cancer in women, and tumor resistance to chemotherapy is one of the major causes of failure in its treatment. A dynamic association between breast cancer and the immune system is essential for this cancer incidence, growth, and metastasis. Emerging evidence suggests that chemoresistance in breast cancer can promote with Toll-like receptors 4 (TLR4), as an important pattern recognition receptor in innate immunity. In this review, we describe the link between TLR4 function and breast cancer chemotherapy. Description: TLR4 expresses not only on immune cells, but also on tumor cells. It can be activated by endogenous ligands produced by host-damaged cells and chemotherapeutic drugs such as paclitaxel. The activation of TLR4 up regulates the expression of various inflammatory cytokines and metalloproteinases that enhance tumor motility and local inflammation. This proinflammatory shift protects cancer cells from death induced by chemotherapy and equips them with the ability to leave the primary site. Also, the release of excessive levels of inflammatory cytokines in the blood can cause bone marrow progenitor cell mobilization to the tumor. TLR4 induced inflammatory after chemotherapy can also promote epithelial-mesenchymal transition. Increased metastatic potential of tumor cells, with high blood and lymph vessel density, results in local recurrence and distant metastasis. On the other hand, stimulation of TLR4 pathway in immune cells might have both pro- and anticancer consequences. For example, the role of TLR4 in antigen- presentation of immune cells and recruitment of cytotoxic T-cells. This may result in decreased primary tumor growth due to chemotherapy-induced TLR4 signaling in BM- derived cells and other immune cells. Discussion and conclusion: TLR4 stimulation in breast cancer chemotherapy has a double-edged sword effect as an anti- or pro-tumoral effect. So further research on the role and mechanisms of TLR4 activation in chemotherapy is needed to improve breast cancer survival rates.
Keywords: Toll-Like Receptor 4, Breast Cancer, Chemotherapy
138 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 25885
Association between Toll-Like Receptor 4 and Response to Chemotherapy drugs in Breast Cancer
Nasim Dana1*, Shaghayegh Haghjooy Javanmard1, Golnaz Vaseghi 2, 1
1. Applied Physiology Research Center and Department of Physiology, Cardiovascular Research Institute, Isfahan University of Medical sciences, Isfahan, Iran. 2. Isfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical sciences, Isfahan, Iran
Introduction: Breast cancer is the most common cancer in women, and tumor resistance to chemotherapy is one of the major causes of failure in its treatment. A dynamic association between breast cancer and the immune system is essential for this cancer incidence, growth, and metastasis. Emerging evidence suggests that chemoresistance in breast cancer can promote with Toll-like receptors 4 (TLR4), as an important pattern recognition receptor in innate immunity. In this review, we describe the link between TLR4 function and breast cancer chemotherapy. Description: TLR4 expresses not only on immune cells, but also on tumor cells. It can be activated by endogenous ligands produced by host-damaged cells and chemotherapeutic drugs such as paclitaxel. The activation of TLR4 up regulates the expression of various inflammatory cytokines and metalloproteinases that enhance tumor motility and local inflammation. This proinflammatory shift protects cancer cells from death induced by chemotherapy and equips them with the ability to leave the primary site. Also, the release of excessive levels of inflammatory cytokines in the blood can cause bone marrow progenitor cell mobilization to the tumor. TLR4 induced inflammatory after chemotherapy can also promote epithelial-mesenchymal transition. Increased metastatic potential of tumor cells, with high blood and lymph vessel density, results in local recurrence and distant metastasis. On the other hand, stimulation of TLR4 pathway in immune cells might have both pro- and anticancer consequences. For example, the role of TLR4 in antigen- presentation of immune cells and recruitment of cytotoxic T-cells. This may result in decreased primary tumor growth due to chemotherapy-induced TLR4 signaling in BM- derived cells and other immune cells. Discussion and conclusion: TLR4 stimulation in breast cancer chemotherapy has a double-edged sword effect as an anti- or pro-tumoral effect. So further research on the role and mechanisms of TLR4 activation in chemotherapy is needed to improve breast cancer survival rates.
Key words: Toll-Like Receptor 4, Breast Cancer, Chemotherapy
139 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 26659
Bioinformatic designing multi-epitope protein of muc1 against cancer
Sahar Akbari1*, Samereh Gholizadeh1* 1. Department of Genetics, Department of Biology, High Institute nurdanesh, Meymeh, Isfahan, Iran. *[email protected] *[email protected]
Introduction &Objectives: One of the effective treatments for cancer patients is biological treatment with epitope vaccines. In vaccine-based cancer immunotherapy, the effort is to restore the immune response to patients and to re-activate the immune system. MUC1 protein is commonly present on the surface of most secretory epithelial cells and is abnormally expressed in most human cancers. The aim of this study was to determine the specific antigenic properties of MUC1 and the bioinformatics design of a multi-epitope protein against cancer. Materials & Methods: Firstly, the Vaxquery and NCBI databases were used to determine the best vaccine candidate sequence in MUC1. Sequence conservation studies were performed by NCBI server and CLC workbench 7.5.1 software. The extracellular part of the protein was determined by the TMHMM server and for evaluating and predicting the secondary and tertiary structure of the protein Phyre2 Server was used. discontinuous and linear epitopes of B lymphocyte cells were determined by IEDB, DISCOTOPE and Ellipro servers. Predicting of MHC class I-associated T lymphocyte epitopes were evaluated on EpiJen and CTLpred servers and MHC class II lymphocyte epitopes were evaluated on ProPred and NetMHCIIpan servers. Antigenicity, toxicity and allergenicity of the peptides were determined respectively by VaxiJen, Toxinpred and Allertop servers. Results: After identifying and scoring discontinuous and linear epitopes, the sequences that were the best candidate for the vaccine were selected. standard Selection for these peptides were high protection, high immunogenicity, no toxicity and no allergenicity. Conclusion: In this study, peptids with high antigenicity were identified to be used as a preliminary design for the development of an effective cancer vaccine. Based on the early stages of bioinformatics screening of the newly designed multi-epitope protein, the designed protein is a suitable candidate for useing as a vaccine. However, further bioinformatics studies are needed to finally confirm this therapeutic vaccine designed for cancer. Keywords: Multi-epitope protein, Antigenic markers, MUC1, Epitope vaccine
140 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 27187
Antioxidant, antimicrobial and antitumor activity of Bacteriocins of Gram- Positive Bacteria
Farzaneh Mohammadzadeh Rostami1, Bahram Nasr Esfahani1*, Saman Shalibeik2 1. Department of Bacteriology and Virology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 2. Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Introduction: Cancer belongs to the main reasons of morbidity and mortality in the world. Lung, liver, colorectal, stomach and breast cancers were the most common causes of death. Bacteriocins are bacterially-produced antimicrobial peptides that have killing activity principally against other relatively closely-related bacteria. some reports have appeared about unanticipated extensions to the generally rather narrow anti-bacterial activity spectrum of some of the LAB bacteriocins and novel applications have been proposed for bacteriocins ranging from controlling the growth of an increasingly-heterogeneous variety of pathogens, including gram-negative multidrug resistant bacteria, viruses, yeasts, and in particular, difficult to control Mycobacterium spp. Description: A systematic search was performed to identify studies which met our inclusion criteria in the Web of Science, PubMed, Embase, Scopus, and Google Scholar electronic databases to the end of December 2019. The estimated economic impact of microbial resistance will be massive, costing nearly 100 trillion US dollars while leading to sharp decreases in the gross domestic product. Microbial resistance against conventional antibiotic agents is a serious hazard to the effective treatment of numerous diseases. Antimicrobial peptides are considered promising alternatives to current antibiotics and have the potential to replace certain antibiotics or to be used synergistically in combination with existing antimicrobial agents. Antimicrobial peptides (AMPs) as antibiotic adjuvant therapy. One of the most intriguing new fields of investigation is the study of bacteriocins as potential anti-cancer agents. Bacteriocins are able to selectively act against cancer cells, most likely due to the distinctive differences in the membranes. Discussion and conclusion: Although Bacteriocins were previously thought to inhibit the growth of only closely related strains or species, but in the recent past they were reported with broad spectrum of antimicrobial activity. In addition, they exhibit selective activity against distantly related bacteria and inhibited the growth of various cancerous cell lines. Recent research indicates that gram positive Bacteria AMPs having anticancer activities against various cancer cell lines.
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 27247
Chicken chorioallantoic membrane as an effective animal modeling for bio molecular studies
Abdolreza Nabinejad1, Rasoul Salehi2 1. Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, (AREEO), Karadj, Iran 2. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Introduction & Objectives: Animal models has been used for biological and medical study, but some ethical and biological aspects limit the whole animal usage for laboratory examinations, meanwhile the possibility of animal escaping during study is noticed by these researchers. This study suggests using of chicken embryo for bio molecular studies for simplicity and list cost. Materials & Methods: Fertile chicken eggs incubate on temperature of 37.7C and 60% humidity with torsion around vertical axes at lists 4 to 6 times a day, then germinal spot on the yolk sac differentiate to morula and followed by formation of a cavity of cellular mass blastocyst, Some cellular layers around the embryos make the allantoic sac and chorioallantoic membrane enclosed to yolk sac at the day of 8 to 9 post incubation. The similarity of the tissue response of chicken CAM with the mammalian models would be amazing for bimolecular and biometastatic study as well as microbial studies. In current study some fertile chicken egg was sanitized and prepared for incubation by a setter machine., After 4 days of in-cubation, the eggs were gently cleaned with a 70% ethanol solution. Using a 5-cc syringe and 18-gauge needle, 2.5 mLof albumen was extracted from the egg. By extracting the albumen, the CAM of the fertilized egg are separated from the top part of the shell, which allows for a small, 1.5-cm2window to be cut in the shell of the egg, without damaging the embryonic structures, the malignant colorectal cancer cells injected to CAM at the day of 7 and the window was then sealed using a transparent tape and the egg was placed back in the incubator until day 9 of incubation. Conclusion:The disseminated tumor cells were assessed in CAM and quantified by PCR. Keywords: CAM, Chick, Model, Bio molecular,
142 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 27753
The growth inhibitory capacity of Thymus daenensis Celak essential oil on MDA-MB-231 cancer cells
Sasan Vakili1, Raheleh Shakeri1, Jalal Khorshidi2 1. Department of Biological Science and Biotechnology, Faculty of Science, University of Kurdistan, Sanandaj, Iran. 2. Department of Horticultural Science and Engineering, Research Center of Medicinal Plants Breeding and Development, University of Kurdistan, Sanandaj, Iran. E. mail: [email protected]
Abstract: Finding new natural cytotoxic agents against human breast cancer cells is valuable. Essential oils are complex mixtures of volatile compounds with various biological properties. Thymus daenensis Celak (Avishan-e-denaei in Persian) is one of the endemic Thymus species in Iran. Yet cytotoxic properties of Thymus daenensis Celak essential oil (TDEO) against breast cancer cells is unclear. In this study, the cytotoxic activity of TDEO against MDA-MB-231 human breast cancer cell line was evaluated by MTT assay. Results showed that TDEO induces cell death in the cancer cells. The anti-proliferative activity of TDEO was time and dose dependent and more pronounced against MDA-MB-231 cancer cell compared to L929 normal cells. Further analysis is required to understand the type of cell death and underlying cellular and molecular anticancer properties of TDEO. Keywords: Breast Cancer; Thyme; Essential oil; MTT
143 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 28702
Evaluation the biodistribution of RGD-modified nanoliposomes containing curcumin in rats by HPLC
Parisa Salehi1, Hamdallah Delaviz*2, Hassan Bardania*2 1. Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran. 2. Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
Introduction: Nanotechnology has many applications in biomedical sciences. Various nano-carriers such as liposomes play a vital role in enhancing the efficacy of drugs and therapies. RGD or (arginine-glycine-aspartic acid) peptide is used to target integrin receptors expressed on cancer cells. In this study, the biological distribution of RGD- modified nano-liposomes containing curcumin was investigated in different tissues of normal rats by HPLC analysis. Method: The nano-liposomes were prepared by lipid thin film hydrolysis. Thirty male Wistar rats were divided into 5 groups of 6. Then, 500 ml of each sample was injected through intraperitoneal (IP) in experimental animals. Group 1: No medication. Group 2: receiving PBS. Group 3: receiving liposome containing curcumin. Group 4: receiving RGD modified liposome containing curcumin and Group 5: receiving curcumin only. The amount of curcumin in different tissues such as blood, liver, kidney, spleen, lung, heart and brain was measured using HPLC analysis after 24 hours of injection. First, different tissues were disrupted using a homogenizer and centrifuged. Then, the amount of curcumin in the supernatant was measured using HPLC analysis. Result: The HPLC results showed that the amount of curcumin in serum tissue was high in the castrated groups (group4> group 3> group 5> group 2 = group 1, and high in liver in group 4> group 5> group 3> group 2 = group 1. It was also high in kidney tissue in group 5 > group 4> group 3> group 2 = group 1. There was not any distribution in other tissues such as heart, lung, spleen and brain tissue. Conclusion: The results showed that the distribution of RGD-modified liposomes containing curcumin was higher in blood, liver and kidney tissues than other groups in other tissues. Keywords: Curcumin, bio-distribution, RGD-modified liposomes
144 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 29625
Isolation and the genetic study of antibiotic-resistant Escherichia coli strains in the urine of patients referred to Ahvaz Veterans Clinic
Yasaman Panahian1*, Parva Nasimi2 1. Department of Biology, Islamic Azad University, Dezful Branch, Dezful, Iran 2. Department of Biology, Islamic Azad University, Tehran Science and Research Branch, Tehran, Iran
Introduction & Objectives: Escherichia coli strains, which produce extended-spectrum β-lactamases (ESBLs), have caused many therapeutic problems due to their resistance to various antibiotics. This study aimed to isolate and determine the pattern of antibiotic resistance of different strains of this bacterium based on the presence of resistant genes including TEM, CTX-M, and SHV in the urine of patients referred to Ahvaz Veterans Clinic. Materials & Methods: ۷۰ Escherichia coli strains were identified and isolated from urine samples of patients and then identified by staining and biochemical analyses. The antimicrobial susceptibility test was performed by the disk diffusion method based on CLSI standards. The presence of SHV, CTX-M, and TEM was shown using Multiplex-PCR, and Rep-PCR also applied to determine the genetic relations between resistance strains. Results: The results among the 11 studied antibiotics showed that the highest and lowest of antibiotic resistance were related to the Nalidixic acid and Cefixime in 72.85 and 64.28% of the strains, respectively, and the Imipenem in 11.24% of the strains. In addition, the frequency of CTX-M, TEM, and SHV genes between resistant strains was 31.42, 12.85, and 57.14%, respectively. These three genes were not present simultaneously in any of the studied strains, but 18 strains had two resistant genes in their genomes. A total of 52 different genetic patterns were found in the resistant strains of Escherichia coli. Conclusion: The results indicate the expansion of the Escherichia coli strains which produce ESBLs and thus the increase of Escherichia coli antibiotic-resistance in the patients' urine. Therefore, the antibiotic treatment of urinary tract infections caused by Escherichia coli in medical centers should be done with more care and after genetic analysis to prevent the increase of such dangerous resistant strains in the community and especially in hospitals. Keywords: Antibiotic resistance, ESBLs, Escherichia coli, Ahwaz.
145 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 29793
Synthesis and Evaluation of Nanofibrous Scaffolds containing bioactive Silibinin on the expression of interleukin-2 in mesenchymal stem cells: possible application in spinal cord injury repair
Vahid barzeghar1, Mehdi Dadashpour2, Nazila Azari3, Akram Firouzi Amandi4, Nosratollah Zarghami5* 1. Department of biology, Faculty of Science, Bonab Branch- Islamic Azad University, Bonab, Iran 2. Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran 3. Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran 4. Asad Abadi Hospital, Center Laboratory Tabriz, Iran 5. Department of Clinical Biochemistry and Laboratory Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Background: Spinal cord injury(SCI) is a nervous system disorder that often results in loss of motor, sensory, and automatic functions. The pathophysiological features of chronic SCI are glial scar and cavity formation: an effective therapy will require contribution of different disciplines such as materials science, cell biology, drug delivery and nanotechnology. One of the biggest challenges in SCI regeneration is to create an artificial scaffold that could mimic the extracellular matrix (ECM) and support nervous system regeneration. Moreover, in remedial medical treatments, the availability of tissue engineering scaffolds that reduce inflammatory conditions is of great interest. The purpose of this study was to evaluate the efficacy of electrospun nanofiber scaffolds containing natural substances with anti-inflammatory properties such as Silibinin to control of inflammation in mesenchymal stem cells (MSCs). Methods: The scaffolds were fabricated based on the polycaprolactone (PCL) containing Silibinin (Sil) as a bioactive material by electrospinning. Fabrication of these nanofibers was confirmed by SEM and FTIR. The proliferation of MSCs on scaffolds was evaluated using the MTT assay. To investigate the anti-inflammatory e ciency of Sil-blended PCL nanofibrous, real-time PCR was applied to measure interleukin-2 expression. ffi Results: MTT results showed increased viability and proliferation of cells on scaffolds containing silibinin over 6 days compared to control. QPCR results showed increased levels of interleukin-2 in mesenchymal stem cells implanted on nanofibers containing Sil. Conclusion: These results demonstrate that Sil-blended PCL electrospun nanofibrous mats appear suitable to support MSCs adhesion and proliferation while concurrently increasing the level of inflammation-related cytokines, therefore representing a hopeful approach for applying in stem cell based regenerative medicine. Keywords: Spinal Cord Injury, Scaffolds, MSCs, Electrospinning, Regenerative Medicine
146
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 30300
Does selenium affect the expression of programmed cell death protein and its ligand in colorectal cancer?
Samane Mohamadzade1*, Mohammad Hassan Emami1,2 1- Poursina Hakim Digestive Diseases Research Center, Isfahan University of Medical sciences, Isfahan, Iran 2- School of Medicine, Isfahan University of Medical sciences, Isfahan, Iran
Introduction: Selenium has the protective role in many cancers, especially in colorectal cancer (CRC); in contrast, programmed cell death protein-1 and its ligand, PD-1/PD-L1, have suppressor effects on anti-tumor immune system responses in CRC and inhibition of these molecules is one of the important purposes in cancer therapy. Therefore, in this study, we aimed to assess the ability of selenium on PD-L1 and PD-1 modulation in the CRC. Description: Multiple electronic databases and conferences were searched up to 2020, to assess the relationship between selenium, PD-1, PD-L1, and CRC. Many studies showed the major role of the immune system in tumor genesis and treatment of CRC. Tumors cells can escape anti-tumor immune responses by overexpression PD-L1; hence the high expression of PD-L1/PD-1 was shown in a group of CRC, named immunogenic CRC. Currently targeting the PD-L1/PD-1 pathway is performed by anti-PD-L1/PD-1 antibodies for reinforcement of anti-tumor immune system responses. On the other hand, it was reported a relationship between selenium and CRC prevention, incidence and therapy. Selenium as an antioxidant micronutrient could neutralize reactive oxygen species, an inducer of PD-L1 expression, at the inflammatory status of cancers. Addition PD-L1/PD- 1 expression could be induced by hypoxia-inducible factor 1α (HIF1α) which is inhibited by Selenium. Similarly, expression of PD-L1 and PD-1 are regulated by some transcription factors and miRNAs which are affected by selenium. Consequently, selenium might be an effective micronutrient for the regulation of PD-L1/PD-1 in CRC. Discussion and conclusion: In immunogenic CRC, PD-L1/PD-1 expression might be regulated by selenium micronutrient through its antioxidant and inhibitory properties for inactivation and inhibition of HIF1α, transcription factors and miRNAs. Accordingly, selenium could be used accompanied with other therapies to reduce the inhibitory effects of PD-1/PDL-1 and reinforce anti-tumor immune system responses.
147
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 30700
Investigation Chondrogenesis of hASCs On The [DCM/ PVA/ Fibrin] Hybrid Scaffolds: In vivo study
Mohsen Setayeshmehr1,2*, Ebrahim Esfandiari2, Batool Hashemibeni2, Mohammad Rafieinia1, Ali Samadikuchaksaraei3, Mohammad Taghi Joghataei3 1- Department of Biomaterials and Tissue Engineering, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. 2- Department of Anatomical Sciences, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 3- Department of Tissue Engineering, School of Advanced Medical Technology, Iran University of Medical Sciences, Tehran, Iran.
Introduction & Objectives: Porous scaffolds derived from native cartilage matrix along with autologous cells could be an effective tool for cartilage tissue engineering (CTE). Recently, it was shown that scaffolds based on cartilage extra cellular matrix (ECM) can induce chondrogenesis of human adipose-derived mesenchymal stromal cells (ASCs) without using exogenous growth factors. However, lack of mechanical properties, rapid biodegradation, and contraction of these scaffolds in culture limit further applications. The present study investigated the fabrication of novel scaffolds based on devitalized costal cartilage matrix (DCM) and poly vinyl alcohol (PVA), using genipin as a natural crosslinker. The scaffolds evaluated in vivo subcutaneously in Vistar rats. Methods and Materials: For this purpose, PVA was modified to expose amine groups (PVA-A), which crosslinked with DCM powder via the lowest genipin percentage of 0.04 % (wt/wt). The crosslinked scaffolds were characterized by different techniques including porosity percentage, pore size, mechanical properties, crosslinking density, and swelling. ASCs were seeded on the scaffolds using fibrin hydrogel. The scaffolds implanted in dorsum of Vistar rats for 28 days. Results: After 28 days the animals sacrificed. Gene expression measurements, biochemical assays and histological staining confirmed that ASC-seeded constructs cultured in the chondrogenic medium can express cartilage-specific genes and synthesize cartilage-related macromolecules. In the presence of TGF-β3 the constructs exhibited significant expression of these markers compared to the control medium. Conclusion: These findings suggest that [genipin-crosslinked DCM-PVA-A/ fibrin] can be considered as an appealing hybrid scaffold for CTE applications. According to the results. the ex vivo strategy can consider as versatile method for cartilage engineering. Keywords: Devitalized Costal Cartilage Matrix, Cartilage Tissue Engineering, Gnipin, poly Vinyl Alcohol.
148
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 30778
Key signaling pathways in papillary thyroid carcinoma
Maryam shirmohamadi1*, Mohammad Yayha Vahidi Mehrjardi1
1. Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. * Corresponding author: ([email protected])
Introduction: Thyroid cancer remains the most common endocrine malignancy worldwide and its incidence and mortality has increased steadily over the last four decades. Improvements in diagnostic techniques that allow a more effective detection of small tumors may account in part for this increase. According to NCCN clinical practice guidelines in Oncology thyroid carcinoma is categorized into four types: papillary thyroid carcinoma (PTC), follicular thyroid carcinoma (FTC), medullary thyroid carcinoma (MTC) and anaplastic thyroid carcinoma (ATC). Description: PTC is the most type of differentiated thyroid carcinoma and accounts for 80–85% of all thyroid carcinomas. PTC can be classified into several variants, among the common of which are the classic variant (CV-PTC), the follicular variant (FV-PTC) and the tall cell variant (TCV-PTC). Genetic alterations in the mitogen-associated protein kinase (MAPK) pathway play an important role in the initiation and progression of PTC, such as , RET/PTC, NTRK (neurotrophic receptor tyrosine kinase) and ALK (anaplastic lymphoma kinase) gene rearrangements or RAS (rat sarcoma) and BRAF (rapidly accelerated fibrosarcoma type-B) activating point mutations, which are almost always found in an exclusive manner, suggesting that oncogenic activation of one member of the pathway is sufficient to drive transformation. Discussion and conclusion: Thyroid carcinogenesis consists of a multiplex process with a large number of molecular alterations among several thyroid neoplasias. The screening for follicular cell-derived specific mutations in association with traditional diagnosis methods has improved the diagnostic accuracy, impacting the prognosis of these tumors. Besides, the advances in the knowledge of the effects of thyroid oncogenes and related mechanisms of action have allowed for the development of multikinase inhibitor targets, promoting new perspectives on therapy to aggressive thyroid tumors. Keyword: thyroid carcinoma, PTC, MAPK pathway, signaling pathway
149 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 32375
Nisin: The Antimicrobial and Anticancer Properties
Saman Shalibeik1, Fereshteh Ghandehari1, Ali-Mohammad Ahadi2*, Farzaneh Mohammadzadeh Rostami3
1- Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran 2- Department of Genetics, Faculty of Science, University of Shahrekord, Shahrekord, Iran 3- Department of Bacteriology and Virology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Introduction: Antimicrobial peptides that are abbreviated AMP. Bacteriocins, peptides or extracellular ribosomal proteins are either bactericidal or inhibitory to bacterial growth and are produced by various microbes. The compounds have made them distinct from antibiotics. The cytotoxic and antitumor properties of nisin cause blockage of head and neck squamous cell carcinoma (HNSCC). Description: A systematic search was performed to identify studies which met our inclusion criteria in the web of science, PubMed, Embase, Scopus, and Google Scholar electronic databases to the end of December 2019. Nisin is an effective antibacterial agent against bacteria including Lactococcus, Streptococcus, Staphylococcus, Micrococcus, Pediococcus, Lactobacillus, Listeria and Mycobacterium spp. The in vitro effects of nisin on the MRSA strain were investigated and it was concluded that nisin is a good candidate for further research on its own or in combination with conventional antibiotics such as vancomycin or ciprofloxacin. Cancer is a complex disease characterized by abnormal cell growth. Significant progress has been made in the treatment of cancer, however, and most treatments include surgery, chemotherapy, and radiation therapy for cells and tissues harmful to the body. Discussion and conclusion: Treatment options for nisin use alone or in combination with other conventional medicines for cancer treatment are still in the early stages. Given the variety of nisin currently available in high purity forms from many commercial vendors, further studies are expected to be published on different applications of nisin. So far, nisin has shown promising laboratory and clinical results as a useful therapeutic agent. In addition, different types of nisin may be combined with conventional drug(s) to promote synergistic results. Although nisin is associated with the development of minimal resistance, however, current findings support the combination of nisin or other bacteriocins in a variety of treatment modalities.
150 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 32804
Fabrication of nano-biocomposite of poly (lactide-co-glycolide)/chitosan electrospun scaffold for transdifferentiation of Schwann-like cells from human adipose-derived stem cells
Shahnaz Razavi* Department of Anatomical Science, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Objective: The transdifferentiation of human adipose-derived stem cells (ADSCs) into Schwann-like cells on biocomposite scaffolds may be a critical issue in nerve regeneration medicine. In this study, tissue-engineered scaffold with chitosan nano powders (CS) and poly (lactide-co-glycolide) (PLGA) was investigated for its potential Schwann cells (SCs) transdifferentiation. Materials and Methods: The differentiation of human ADSCs into S-like cells was induced with different CS content and direction of nanofibers on PLGA/CS scaffolds. Cell morphology and proliferation of differentiated cells were investigated by scanning electron microscopy and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay respectively. For assessment efficiency of transdifferentiation, the expression of SC markers (glial fibrillary acidic protein and S100), and myelinogenic marker (myelin basic protein) were investigated in different nanochitosan content and direction of nanofibers scaffolds, using immunocytochemistry technique. Results: The nanochitosan can significantly promote cell proliferation of differentiated cells (p< 0.05). The mean percentage of S-like cells on greater CS content nanofibers scaffold was significantly higher than others (p< 0.05). In addition, the align orientation of nanofibers in scaffolds guided the differentiation of ADSCs toward myelinating S-like cells on the constructs. Conclusion: Overall, we found that high CS content and aligned-orientation of nanofibers in biocomposite scaffold (70/30A) can promote differentiation and myelinogenic capacity of S-like cells induced from human ADSCs. Keywords: ADSCs, PLGA/CS, Nanocamposite, transdifferentiation, Schwann-like cell.
151 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 33073
Enrichment of differentially expressed eRNAs in cancer-related pathways in gastric cancer
Basireh Bahrami1, Parvaneh Nikpour1, *
1. Department of Genetics and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Introduction & Objectives: Enhancer RNAs (eRNAs) are a class of the non-coding RNAs that are involving in the regulation of gene transcription. Alterations in eRNAs expression has been reported in several cancer types. In the current study, we utilized eRic database to analyze the differentially expressed eRNAs in gastric cancer tissues from TCGA cohort to determine the molecular mechanisms involved in tumorigenesis. Materials & Methods: Differentially expressed eRNAs were selected based on their fold change and adjusted p-values, which were provided by the eRic database based on the data from gastric cancer samples of TCGA cohort. The inclusion criteria were set as follows:
false discovery rate (FDR) < 0.05 and |logR2R(fold change)| > 1. To understand the biological meaning behind the large list of upregulated eRNAs, target genes of them were extracted and enrichment analysis was performed using Enrichr tool. Results: In total, 724 differentially expressed eRNAs (693 upregulated and 31 downregulated) were identified in gastric cancer in comparison with normal tissues. Then, we determined target genes of the upregulated eRNAs which were 859 genes. Our results revealed that these target genes were significantly enriched in various biological processes of Gene Ontology (GO) terms such as regulation of gene expression (GO: 0010468), regulation of mRNA processing (GO: 0050684), regulation of transcription from RNA polymerase II promoter (GO: 0006357) and associated with cellular components such as RNA polymerase II transcription factor complex (GO: 0090575), nuclear transcription factor complex (GO: 0044798) and nuclear chromatin (GO: 0000790). Pathway analysis showed that the genes were mainly involved in tumorigenesis-associated pathways such as Wnt Signaling Pathway, Gastric Cancer Network and EGF/EGFR Signaling Pathway. Conclusion: These findings increase our understanding of the molecular mechanisms of gastric cancer mediated by eRNAs and will aid in identifying potential targets for diagnostic and therapeutic usage. Keywords: Enhancer RNAs, Non-coding RNAs, Stomach neoplasms
152 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 34104
Epigenetic markers; a review of promising milieu for personalized diagnosis and treatment of osteoporosis
Sadegh Baradaran-Mahdavi 1, Roya Kelishadi 2
1. Clinical Scientist resident, Child Growth and Development Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran 2. Professor of Pediatrics, Child Growth and Development Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract: Osteoporosis is characterized by low bone mass and increased susceptibility to fragility bone fractures due to alterations in bone micro-architecture. It is mostly observed in the elderly and in post-menopausal women. In osteoporosis, the bone remodeling process, alters in favor of bone resorption and causes the clinical presentation of the disease. The role of genetic profile in bone phenotype and disease-related fracturein osteoporosis is well-documented. However, it is proposed that less than 10% of observed variance in bone mineral density, could be related to genetic factors.With respect to developmental origins of osteoporosis, emerging evidence has been focused on the interaction of environmental and genetic factorsfrom antenatal period to adulthood. This interaction may be modulated, in part by epigenetic mechanisms including DNA methylation, histone modifications and microRNAs (miRNAs). Experimental and observational studies represented different aspects of epigenetic mechanisms involved in bone cell differentiation and bone homeostasis. While, conventional techniques such as dual-energy x-ray absorptiometry (DEXA) or medications such as oral or parenteral bisphosphonates are still being applied for diagnosis and treatment of osteoporosis respectively, identifying the epigenetic biomarkers may offer promising personalized diagnostic techniques or therapies. The alterations in miRNAs and DNA methylation patterns have been associated with osteoporosis onset and progression in many studies. However, the robust research is scare in this field and sensitivity or specificity of any biomarker as well as the potential of applying new treatments based on genetic/epigenetic pathways, needs to be thoroughly investigated in the future. The present review considers the available evidence of epigenetic of osteoporosis with a special focus on the most recent studies proposing new biomarkers to diagnose or prognosticate the disease or investigating potential genetic/epigenetic based treatments for this multifactorial condition in the clinical setting. Keywords: Osteoporosis,Epigenetics, Biomarkers, DNA methylation, miRNAs, Histone modifications
153 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 34335
Association between genetic markers of PCOS1 region and polycystic ovarian syndrome
Ali Kowsari1, Naser Kalhor1, Faeze Davoodi1, Leila Naserpoor2, Hoda Fazaeli1* 1. Department of Mesenchymal Stem Cells, Academic Center for Education, Culture, and Research (ACECR), Qom Branch, Iran 2. Department of Reproductive biology, Academic Center for Education, Culture, and Research (ACECR), Qom Branch, Iran * Corresponding Author
Background: Polycystic Ovarian Syndrome (PCO) is one of the most common endocrine disorders in women. This syndrome is a complex and heterogeneous disorder with unknown reasons, but there are strong pieces of evidence for its classification in genetic disease. It has shown that the gene region called PCOS1 – located on the short arm of chromosome 19- is a proper candidate to assess the risk of PCO. In this study, we evaluated the polymorphisms of 9 STR markers (D19S865, D19S906, D19S840, D19S410, D19S212, STR2b, STR6b, STR5b ، D19S391 and D19S884) in this gene region and the risk of PCO in 110 women attending to highly specialized jihad daneshgahi infertility treatment center was studied using PCR and sequencing techniques. Methods: peripheral blood samples were taken from healthy and PCOS individuals, then primers and PCR were designed for STRs and then the samples were sequenced. Results: The obtained results of the demographic and hormonal analysis showed that the levels of estradiol, prolactin, LH and FSH and smoking were not significantly different between patients and healthy groups, but in case of AMH, family history, FBS and BMI there were a significant difference between the two groups. Examination of STRs in the genome of patients and healthy individuals also showed that only 2 of 7 studied STR markers (D19S865 and D19S212) were significantly associated with the risk of PCO. Conclusion: Regarding o the sensitivity and specificity of the PCOS1 region in the development of PCO, it can be concluded that this region is also important in the Iranian population as a key area associated with PCO. Therefore, by evaluating these regions, we can obtain an acceptable prediction of PCO. Keywords: PCO, STR, Genetic marker
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 34416
The effect of gassericin A on the gene expression of tumor Necrosis Factor alpha (TNF-α) in differentiated preadipocyte 3T3-L1 cells
Danial Yaghoubi1, Hamid Reza Kazrani2*, Hesam Dehghani2, Mehrnaz Rad3
1. Department of physiology, the School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran 2. Department of Biotechnology, the School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran 3. Department of Microbiology, the School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Introduction & Objectivesc: clinical studies report a growing increase in the mortality caused by obesity and obesity-related diseases. There is a close link between obesity, adipocyte inflammation and colon cancer, that may threaten health. Factors such as nutrition, genetic background, and activity may affect adipocyte metabolism. One of the other factors which has been recently studied is intestinal microbiota. Microbiota refers to commensal microbial population in the body. The highest population of microbiota has been observed within the colon. These microorganisms produce different compounds such as short chain fatty acids, lipopolysaccharides and bacteriocins. Bacteriocins are peptide composed of different amino acids, which are released to control the other microorganisms within the intestine. One of these peptides is Gassericin A, which has a cyclic structure containing 58 amino acids. This bacteriocin is produced by the intestinal Lactobacillus gasseri LA39. According to the present databases, there is no report regarding the effect of this bacterial product on metabolic processes. Materials & Methods: In this study, L gasseri was cultured in Dob MRS broth media. Gassericin A was extracted using column chromatography (HIC/RP-8). Thereafter, the peptide was verified according to its specific antibacterial effect on L delbrueckii bulgaricus and also employing tricine-SDS-PAGE method. To investigating the possible effects of gassericin A on the expression of the TNF-α gene, the preadipocyte 3T3-L1 cells were first allowed to differentiate into adipocytes using IBMX, dexamethasone, and insulin. The gene expression of TNF-α was assessed using quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay. Results: Gassericin A cause a significant reduction in the expression of TNF-α gene in adipocytes. Conclusion: This study suggests that gassericin A produced by Lactobacillus gasseri LA39 prevents inflammation in adipocytes via downregulation of TNF-α. This process may play role in prevention of obesity and cancer. Keywords: microbiota, Lactobacillus gasseri, gasseircin A, TNF-α, 3T3-L1 cell
155 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 34882
Extraction and Investigation some of Biological Characteristics of Venom Rhopilema nomadica in the Persian Gulf
Somayeh Ketabdari1*, Hamidreza Goudarzi 2, Zahra Salehi Najaf Abadi 3, Hossein Zolgharinin 4, Ahmad Savari5 1. student of PhD Marine Animals, Department of Biology, Khorramshahr Marine Science and Technology University: [email protected] 2. PhD in Nanobiotechnology, Faculty Member of Razi Vaccine and Serum Research Institute: [email protected] 3. PhD in Biotechnology, Faculty Member of Razi Vaccine and Serum Research Institute: [email protected] 4. PhD in Molecular Biology, Faculty Member of Khorramshahr University of Marine Science and Technology: [email protected] 5. PhD in Oceanography Faculty of Marine Science and Technology Khorramshahr University: [email protected]
Abstract: Jellyfishesarethe species of invertebratesbelong toCnidaria’sclass. The cnidocyte cells as prey hunter system of jellyfishes are located on their arms, around the mouth or other parts of body. Each bitingcell contains a corneous nematocyte and a capsule containing a spring hollow filament, andproper stimulator cause the nematocyte to be thrown abruptly. There are numerous reports of victims by different kind of jellyfishes.The sign and symptoms such as skin redness, shortness of breath, low blood pressure and sometimes death occurs fallowing jellyfishes bite. Therefore, the venoms ofjellyfishes are interestedby scientists in pharmaceutical research. The aim of this survey was to optimizethe method of venom extraction and study of some biological aspects of venom fromRhopilemanomadicaspecies such as total protein, molecular weight of venom components and toxicity (LD50). The samples were collected from Persian Gulf (around Qeshm Island) andtransferred to laboratory,then their tantacules were separated andwere frozen in -20 Co. The frozen tantacules were lyophilized. The venom extraction was performedbyfreeze& thaw method. Thetotal protein was 1.2 mg / ml, by Brad-Ford assay. SDS-PAGE detected three protein components with 170, 130 and 53 KDa, respectively.The toxicity test (LD50) was performed onBalb-c mice via intradermal (ID) rout and resulted 32μg/kg.Development of research on biological aspects of this jellyfish venom can prepared essential knowledge for future studies such as antivenom production and clear approaches to cure victims due tojellyfishes bites. Keywords: Jellyfish, Nematocytes, Venom, Rhopilemanomadica
156 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 35539
Comprehensive transcriptomic analysis reveals a relation between expression alteration of transcribed ultraconserved regions (T-UCR) and hypoxia
Anis Khalafiyan1, Parvaneh Nikpour1, * 1. Department of Genetics and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Introduction & Objectives: The transcribed ultraconserved regions (T-UCRs) are noncoding RNAs that are nearly 100% conserved between the genome of three species: human, rat and mouse. Selective conservation of these sequences during evolution suggests that these areas are important and necessary elements for proper cell function. UCRs are often located in the fragile sites and cancer-associated genomic regions. Therefore, it is suggested that these regions may be candidate genes for cancer susceptibility. Hypoxia has been recognized as one of the fundamentally important features of many malignancies and plays a critical role in many aspects of cancer biology, including cell proliferation, angiogenesis, immunosurveillance, metabolism, and tumor invasion and metastasis. Here, we aim to investigate the expression profile alterations and potential function of all T- UCRs under hypoxic conditions. Materials & Methods: RNA-seq raw data were downloaded from Array-Express database (E-MTAB-2580). The data were then aligned to the human genome reference (hg19) by using HISAT2, then htseq-count was utilized to quantitate the number of reads mapping into each T-UCR. Differential expression analysis was then performed using DESeq2 to identify deregulation of T-UCRs. The fold change (FC) values of individual T-UCR levels were calculated and differentially expressed T-UCRs with |log2(fold change)|> 1 and adjusted P-value < 0.05 were considered to be statistically significant. Results: In the present study, we compared RNA sequencing data set of 12 human embryonic kidney (HEK293) cells exposed to normoxia compared to hypoxia conditions for 24 hours. Using the cut-off criteria, we identified T-UCRs that were differentially expressed in the hypoxia and then using PANTHER ontological analysis, we classified hypoxia-induced and repressed genes according to the gene ontology. Conclusion: This type of studies increases our understanding of the complex mechanisms of hypoxia mediated by T-UCRs and will aid in identifying potential candidates for pharmacological intervention of cancer.
157 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 35961
Hypoxia-targeted therapies for patients with nonsmall cell lung carcinoma
Saba Talebian1, Fatemeh B. Rassouli1,2 1. Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran 2. Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University
Introduction: Based on pathoclinical characteristics, nonsmall cell lung carcinoma (NSCLC) accounts for approximately 90% of all lung cancers. Due to inadequacy of current therapeutic modalities, mortality rate of NSCLC is high, which emphasis on designation of new and more effective strategies. Hypoxia, defined as oxygen tension below physiological normoxia, is nearly exclusively restricted to cancer cells. Since hypoxia induces migration and metastasis of malignant cells, it is involved in treatment resistance and poor survival. In this regard, hypoxia-targeted therapies, alone or in combination with other modalities, would be associated with favorable clinical outcomes in NSCLC. Methods: Recent articles including Keywords hypoxia biology; nonsmall cell lung carcinoma and targeted therapy were extracted from databases PubMed and Web of Science to review recent advances in hypoxia-targeted therapies for NSCLC patients. Results and Conclusion: There are several options to target hypoxia; increase in oxygen delivery by agents like efaproxiral, decrease in oxygen consumption by drugs such as metformin, and use of hypoxia-activated toxic prodrugs such as evofosfamide to specifically target hypoxic cells. Based on reported trials, improved clinical outcome in hypoxia-targeted therapies would be observed if hypoxia is present in NSCLC, and this approach could counter with deleterious effects of hypoxia. It is obvious that more research is still required to develop other effective and less toxic hypoxia-targeted therapies, and efficacy of this strategy must also be investigated in combination with radiotherapy and chemotherapy to achieve best clinical outcomes. Keywords: Hypoxia biology, Nonsmall cell lung carcinoma, Clinical outcome, Targeted therapy.
158 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 36108
Inhibition of MDA-MB-231 cancer cell proliferation by Crocus cancellatus subsp. damascenus corm methanol extract
Bahram Savari1, Raheleh Shakeri1, Jalal Khorshidi2 1. Department of Biological Science and Biotechnology, Faculty of Science, University of Kurdistan, Sanandaj, Iran. 2. Department of Horticultural Science and Engineering, Research Center of Medicinal Plants Breeding and Development, University of Kurdistan, Sanandaj, Iran. Abstract: Breast cancer is a life-threatening illness that resulting in women deaths worldwide. Despite significant advances in cancer treatment, mortality from breast cancer still exist. Current chemotherapy drugs have different side effects such as drug resistance and apoptosis induction in healthy cells. Hence, effective and better tolerated drugs are needed. For the past 30 years, natural compounds have been great attention due to value biological properties. In this study, the anticancer properties of Crocus cancellatus subsp. damascenus against MDA-MB-231 human cancer cell line was investigated. The methanol extract of Crocus cancellatus subsp. damascenus corm was prepared, dissolved in DMSO, and diluted. The cytotoxicity of the diluted extracts was evaluated by MTT assay. Data analysis showed a significant decrease in the number of viable cells with increasing concentration of the extract and incubation time. The extract also suppressed the viability of MDA-MB-231 multicellular spheroids (3D). In conclusion, the methanol extract of Crocus cancellatus subsp. damascenus corm contains anti-proliferative agent(s). Keywords: Breast Cancer; Anticancer; Crocus
159 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 36852
Single Step Smart Sensing of Proteinmarkers Using DNA Strand Displacement and Split G-Quadruplex Enhanced Fluorescence
Mehrnaz Mehrabipour1, Hadi Ravan1, Sajad Rajabpour1 1. Department of Biology, ShahidBahonar University of Kerman, Kerman, Iran
Abstract: Proteins are one of the most common biomarkers for cancer diagnosis. Therefore, creating new methods that allow fast, cost-effective and simple detection of proteins are important. In general, ELISA and Protein Array are versatile approaches for specific protein detection; however, they are consisting of considerable drawbacks including limited dynamic ranges, inadequate sensitivity and being time-consuming. Accordingly, due to nanotechnology developments in the diagnostic application, we proposed a DNA nanotechnology approach to design a DNA-based gate which functions based on an aptamer system for protein detection using strand displacement reaction. Besides, we have utilized an enhanced output signal in the designed system for highly sensitive detection of protein marker. In this study, all of the oligonucleotides have been designed using mFold software, and after the construction of DNA nanostructures, detection of protein biomarker was conducted by Tioflavin T. The results open avenues for the development of new DNA- based devices for sensing of different protein biomarkers in cancer prognosis. Keywords: Cancer Prognosis, DNA Strand Displacement, Protein Biomarker
160 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 36950
Effect of Cartilage ECM on polycaprolactone/Fibrin hybrid scaffold fabricated by salt leaching method
Ali Honarvar1, Ali Valiani2, Batool Hashemibeni2, sho,leh ghaedamini2, Saeed Karbasi3, Mohsen Setayeshmehr3 1- Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 2- Department of Anatomical Sciences, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 3- Department of Biomaterials, Tissue Engineering and Nanotechnology, School of Advanced Medical Technologies, Isfahan University of Medical Sciences, Isfahan, Iran b.
Introduction: The Ideal three dimensional (3-D) scaffold in Cartilage tissue engineering should be biocompatible and biodegradable to enhance cell attachment, adequate mechanical properties and high porosity with suitable interconnected pores to improve cell ingrowth and nutrient transportation. Salt leaching technique include making a solution of polymer in solvent and mix salt particles of a fixed diameter as porogen to produce a homogenous suspension and fabricate a porous structure. The main advantage of using cartilage ECM derivatives as a scaffold is its ability to maintain growth factors. Therefore, such ECM could potentially be used as a carrier for freshly isolated stem cells, with such a construct forming the basis of a single-stage therapy for articular cartilage regeneration. Materials and Methods: In this study, PCL scaffold was prepared with salt leaching method. ECM was mixed with fibrinogen solution in 2% (w/v), 5% (w/v) and 10% (w/v). ADSCs added to ECM/ fibrinogen solution and seed on the PCL scaffolds in different groups that cells inhabited in the scaffold porosity. Then thrombin was added to this complex and allowed to gel. MTT assay, water absorption, scanning electron microscopy and dynamic mechanical analysis were used to characterize the mechanical properties of the resulting scaffolds. Results: data showed that stem cell can proliferate well on PCL/Fibrin scaffold coated with ECM and a PCL salt-leached scaffold had a compressive modulus of 0.2 – 0.5 MPa. ECM 2% group presented a high significant cell viability that was suitable for cell ingrowth compare with ECM 5% and ECM 10%. Conclusions: when the ECM concentration was increased (10% w/v), the structure cannot preserve its primary shape, consequently using of 2% (w/v) ECM embedded in PCL/Fibrin hybrid scaffold is recommended. Keywords: polycaprolactone, salt leaching, scaffold, tissue engineering
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 38012
The effect of Platelet Rich Plasma on proliferation and survival of Adipose – Derived Stem Cells in Fibrin and Alginate scaffolds
Batool Hashemibeni, Marzie Sadeghian, Reihane Seyed Ebrahimi Department of Anatomical Sciences, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Background: Today, stem cells are the best candidate for cell therapy and tissue engineering. In this study, proliferation and viability of ADSCs affected by PRP (Platelet Rich Plasma) in Alginate and Fibrin scaffolds were evaluated and compared. Materials and Methods: ADSCs in third passage, were encapsulated in fibrin and alginate scaffolds and affected by medium with PRP10% or FBS10%. Then cell viability evaluated by MTT assay and Trypan blue staining. The apoptosis was assessed by Annexin V-FITC Kit on fourth and eighth days. Data analyzed by ANOVA (one-way). Results: The MTT and Trypan blue staining results showed that the rate of viability in fibrin with PRP was higher than other groups significantly (P<0.05). Percentage of early apoptotic cells in fibrin with PRP was significantly lower than other group (P<0.01). Late apoptotic cells were not significant different between the groups. Discussion: The PRP has several advantages compared with recombinant growth factors and animal serum and can be considered as suitable supplement for stem cell culturing in compared with FBS. Keyword: ADSCs, PRP, Fibrin, Alginate
162 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 38022
Laser Therapy
Zahra Germani 1(*), Farzane Jabari 2 , Reza salarian 3
1. Department of Biomedical engineering (Biomaterial), Maziar Higher education institute,Royan,Iran 2. PhD Biomedical engineering (Biomaterial), Nanotechnology and Advance Material Department, Material Energy research center (MERC) Alborz,Iran 3. Department of Biomedical Engineering (Biomaterial), Maziar Higher Education Institute, Royan, Iran Background and objective: Laser therapy has been shown to stimulate the regeneration of bone, blood, the lining of blood vessels, cartilage, cochlear hair cells, central and peripheral nerve, and muscle. Moreover, it has been documented to enhance the quality of healed tissue. Laser therapy may be an ideal treatment. It may not only effectively address many medical conditions but also has been widely reported to improve health and wellbeing as evidenced by a host of biological markers. Search method: In the context of this overview, 13 articles were used between the years 2015 through the end of 2018 using the Keywords of PubMed, Google scholar search engines. Results: Laser therapy energizes living systems. Four well accepted effects in the scientific literature are: Bio stimulation / Tissue Regeneration Reduction of Inflammation Analgesia Enhanced Immune Function / Antimicrobial One important way in which laser therapy adds energy is through photon absorption by mitochondria. These tiny organelles which have been called the "powerhouses" of the cell, are found in most plants and animals. Mitochondria are able to absorb laser light which then activates a series of reactions to increase and store more cellular energy in the form of adenosine triphosphate .By increasing energy available in this readily accessible form, laser light is able to greatly stimulate the biological function of cells, tissue, and systems and even raise overall vital energy throughout the individual. Conclusion: The majority of authors report that laser therapy speeds up the process of tissue repair, but further studies are suggested to determine the best parameters to be used
Keywords: Laser therapy, Tissue regeneration, Cells
163
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 38123
The Effect of Estrogen in Diffuse Axonal Injury: A Randomized Clinical Trial
Nazanin Sabet1, Zahra Soltani2, Hamideh Dannesh1, Saeed Karamouzian2 1. Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran 2. Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Objective: Neuroprotective effect of estrogen in traumatic brain injury (TBI) has been shown in many animal studies. This clinical trial was designed to investigate the effect of estrogen on neurological outcome in diffuse axonal injury (DAI). Materials and Methods: Frothy- eight male patients 18-60 years with moderate to severe DAI admitted within the first 4 hours of injury were randomly divided into control and estrogen groups. The estrogen group received orally 1.25 mg conjugated estrogen within four hours of brain injury and four days consecutive in addition to standard treatment. Glasgow coma scale (GCS) assessment was performed at the time of admission, six days after injury and the day of discharge, while functional independence measure (FIM) and Glasgow coma scale (GOS) at the time of admission and the third and sixth months after injury. Results: Although there was no significant difference in GCS between the control and estrogen groups at six days’ post injury, this score in the estrogen group was higher than the control group at the time of discharge (P <0.05). There was an increase in GOS score in the estrogen group compared to the control group at three and six months after DAI (P <0.05). FIM score was not significant between the control and estrogen groups at three and six months after injury. This score increased in the estrogen group at six months after injury compared to three months after injury (P <0.001). Unlike the control group, no death occurred in the estrogen group. Conclusion: The findings of this study showed the treatment of estrogen could probably improve neurological outcome in male patients with DAI. A study with larger sample size is needed to confirm the efficacy of estrogen in DAI. The mechanism of the effect of estrogen in DAI will be examined in a future study. Keywords: Estrogen; Neurological Outcome; Diffuse Axonal Injury; Glasgow coma scale; Functional Independence Measure; Glasgow coma scale
164
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 39665
Immunomodulatory effects of Cinnamomum against COVID-19
Zahra Sadri1, Mehrdad Zeinalian1,2 * 1 Ala Cancer Prevention and Control Center, Isfahan, Iran 2 Department of Genetics and Molecular biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract: Coronavirus disease 2019 (COVID-19) is a kind of viral pneumonia which its pathogenesis poorly understood. The similar mechanisms of SARS-CoV and MERS-CoV can give us information about the pathogenesis of SARS-CoV-2 infection. The immune system plays a vital protection role against infectious diseases and is affected by a variety of plants including vegetables and spices. Some experimental trials on the properties of Cinnamomum indicate that this spice may have anti-inflammatory, antimicrobial, antioxidant and immunomodulatory effects. Cytokine storm resulting from the release of large amounts of pro-inflammatory cytokines and chemokines, including IL-1 β, IL-2, IL- 6 , IFN- α, TNF-α, TGF β, etc. Increased levels of IL-6 are a stable indicator of poor outcome in COVID-19 severe cases with pneumonia and acute respiratory distress syndrome (ARDS). Cinnamaldehyde pretreatment dose-dependently reduce inflammation evidenced by decreased TNF-α, IL-1β, and IL-6 levels against intestinal tissues injury, Wich is able to inhibit intracellular signaling induced by PAMPs recognition. This inhibition could decrease the circulation of the inflammatory process. The treatment also significantly represses oxidative stress, NF-κB activation and downregulated apoptotic protein expression including p53, Bax, caspase-9 and caspase-3, and restoring Bcl-2 against tissue injury. The flavonoids from C. cassia twig volatile oil effectively promote IL-1β and IL-6 release from murine macrophage cell line. Thirty-two patients with orofacial granulomatosis diagnosis- a nonspecific granulomatous inflammation presenting as facial or lip swelling, cheilitis, ulcerations- had received a cinnamon and benzoate-free diet as primary treatment for a period of 8 weeks. After that patients on the diet had a significant improvement in oral inflammation. The results of different investigations suggest that species of Cinnamomum and affective materials has the conflicting effects in different situations and exact effect of that on the viral infections remains unclear. Key words: Covid-19, Immunomodulatory, Cinnamomum
165
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 39673
Isolation of methicillin-resistant Staphylococcus aureus from urine in patients referred to Ahvaz Pasteur Laboratory
Neda Dahimavi1*, Parva Nasimi2 1. Department of Biology, Islamic Azad University, Dezful Branch, Dezful, Iran 2. Department of Biology, Islamic Azad University, Tehran Science and Research Branch, Tehran, Iran
Introduction & Objectives: Antibiotic resistance in Staphylococcus aureus infections as a major public health problem causes the prevalence of resistant urinary tract infections in clinics and hospitals. Towards this purpose, we analyzed the urine sample of patients at Pastour clinic in Ahwaz between August 2016 and August 2017. Materials & Methods: 132suspected urine samples were evaluated by biochemical methods including catalase, mannitol fermentation, coagulase and DNAse for the presence of staphylococcus aureus. The sensitivity test was performed using disc diffusion method for common antibiotics. Data were analyzed using descriptive statistics . Results: The results of biochemical tests confirmed the presence of 84 different strains of Staphylococcus aureus in the urine of the studied patients. The maximum antibiotic resistances were related to penicillin with 100% and cefoxitin (methicillin) with 90.47% and the minimum antibiotic resistances was related to nitrofurantoin with 4.76% In addition, other antibiotics including trimethoprim-sulfomethoxazole, tetracycline and vancomycin antibiotics showed 88.09%, 83.33% and 10.71% resistance, respectively. Conclusion: Methicillin-resistant Staphylococcus aureus strains (MRSA) are one of the leading causes of nosocomial infections that may even lead to patient death. The results of this study indicate the prevalence of resistant strains of this bacterium in Ahvaz as well as the need to increase hospital hygiene and use appropriate antibiotics with low resistance to prevent further spread of these dangerous strains. Keywords: Methicillin-resistant Staphylococcus aureus, Urinary Tract Infection, Antibiotic Resistance, Ahvaz.
166 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 40131
The Association of Anemia with Learning and Memory in Students of Imam Raouf Educational Complex of Isfahan, 1397
1 1* 1 Meysam Ghanei , Shima Toghiani , Akbar Karimi 1. department of Cellular and developmental biology . Payame Noor University of Isfahan. Corresponding Author: shima toghiani, mt.toghiani@ yahoo.com
Background and Aim: Individuals with iron deficiency, even without anemia, always feel tired. They are not interested in doing their duties, so their efficiency will be less than healthy individuals. According to the widespread prevalence of iron deficiency, especially in boys and girls, it has a profound effect on all levels of society, including health, economics, education, and so on. The aim of this study was to determine the prevalence of iron deficiency anemia and its effect on learning and memory in students of Imam Raouf Educational Complex. Material and Method: In this study, a multistage (clustered and classified) random sampling method was used. It was performed on 300 students (181 girls and 119 boys) who were randomly selected. Data collection tools were questionnaires and blood tests (HB, HCT, MCV, and Ferritin). To obtain the results, SPSS22 software and Chi-square test were used. Results: The results of this study showed a significant relationship between serum ferritin, hemoglobin, and hematocrit with levels of learning disorder in students (P = 0/000). There was no significant relationship between BMI and sex with the levels of learning disorder in boys and girls (P = 0.09, P = 0.2). Conclusion: Regarding the relatively high prevalence of iron deficiency and the fact that anemia can interfere with learning, verbal, and behavioral skills and also reduction in optimal intelligence, it is recommended to evaluate the children with laboratory blood tests such as hematocrit, hemoglobin, and ferritin before entering primary school in order to diagnose anemia due to iron deficiency. It is also recommended to study different age groups and sex with greater number of participants and longer period of time in order to examine their function. Keywords: Iron deficiency, Anemia, Primary school students, Learning
167 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 40167
Wound healing effects of ZnO nanocomposite containing Alchemilla extract in rats
Danial Bajgiran1, Mahdi Bayrami1, Moin Bajgiran2, Amin Bayrami2, Sina Nagavian2, Amirhossein Soltani2, Yasin Qabool3, Amin Bayrami4, 1. Department of Biology, Faculty of Science, University of Mohagheghe Ardabili, Ardabil, Iran. 2. Department of Biology, faculty of science, Kavian Institute of Higher Education College, Mashhad, Iran 3. Department of Biology, faculty of science, Mashhad branch, Islamic Azad University, Mashhad, Iran. 4. Faculty of medicine science, Sari branch, Islamic Azad University, Sari, Iran.
Introduction & Objectives: Wound healing still remains a challenging clinical problem for which efficient wound management is necessary. there is a desire for novel strategies to achieve expeditious wound healing because of the enormous financial burden worldwide. in recent decades, researchers have focused on the development of nanotechnology. drug delivery systems are expanding to meet the specific goal and increasing the burden of treatment using nanotechnology. to minimize wound infections, they often use disinfectants to coat the wound. The aim of this study is investigation of ZnO and ZnO containing Alchemilla extract effects on wound healing. Materials and Methods: In the present study, ZnO metallic nanoparticle (NP) via Alchemilla extract (Ext) and one without Alchemilla namely: ZnO/Ext and ZnO (w) were prepared by microwave method and assessed for their wound healing activity on excision experimental model of wounds in rats. The developed nanocomposites have been characterized by physico-chemical methods such as; X-ray diffraction (XRD), Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR). The wounded animals dedicated to ZnO, ZnO/Ext and control groups. In all groups, the wounds were treated by prepared samples for 3 weeks. Every seven days, after measuring decreasing rate of wound size, three samples from each group were separated for histopathological analysis. Results: The in vivo evaluation reveals that the ZnO/Ext nanocomposite dressing enhances the wound healing and promotes re-epithelialization. Conclusion: The results showed that the ZnO/Ext is significantly the most effective material in comparison with ZnO (W) and control. Keywords: Nano-biotechnology, wound healing, zinc oxide, Alchemilla
168 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 41035
Overview of microRNA targeting
Samira Nafar1 1. Department of medical genetics, Faculty of medicine, University of medical science, shiraz, Iran
Introduction: MicroRNAs are post-transcriptional gene regulations. They function through binding in their target sequence in mRNAs. Thanks to significant effect of them, determining of their gene targets have shed light on molecular etiology of disease so would be useful in diagnosis and treatment. There are lots of bioinformatics databases like mirwalk (http://mirwalk.umm.uni-heidelberg.de/), mirTarBase (http://mirtarbase.mbc.nctu.edu.tw/), pictar (https://pictar.mdc-berlin.de/), TargetScan (http://www.targetscan.org/), which forecast target genes via matching of seed sequence of microRNAs in microRNA recognition element (MRE) and sequence of untranslated region of genes (UTRs). One of valid experiments to confirm this targeting is luciferase reporter gene assay test. Description: In-silico study to predict of possible targets of miRNA of interest were done. 3 UTR of mRNA consist of seed sequence were amplified from genomic DNA by PCR and was cloned into Psicheck construct, which expresses firefly luciferase by Xho1 and Not1 enzymes. HEK293 cells were seeded and co-transfected with 0.1 µg firefly luciferase reporter containing the gene 3'UTR and miRNA and mutant 3 UTR of gene as positive control, along with Renilla luciferase vector pRL-TK as an internal control using Lipofectamine 2000. All transfections were performed in two experiments, each in triplicate. Luciferase activities were measured according to the manufacturer’s instructions (Dual-Luciferase Assay System; Promega). Discussion and conclusion: Targeting of 3 UTR of gene of interest through miRNA led to an intense reduction of the luciferase signal. Mutations of miRNA binding sites in the 3′UTR reversed the inhibition and the signal of luciferase in positive and internal control is about equal. Use of bioinformatics and luciferase assay as tool for detecting of miRNAs targets can assist to determine the power of post-transcriptional regulators in targeted cell therapy by reduce or over expression of them to control their gene targets along with important molecular pathway in regenerative medicine field.
169 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 41439
Bioinformatics evaluation of targetomhas-miR-143-3p signaling pathways and related function ofHIF-1α, SNP(rs11549465) in patients with acute lymphoblastic leukemia
Behnaz Hamzei1, Massoud Houshmand2, Atefeh Zamani3, Nasrin Fatahi3
1. Cellular-Molecular Division, Department of Biology, High Institute Nurdanesh, Meymeh, Isfahan, Iran 2. Medical Genetic Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran 3. Gene Raz Buali,Genetic and Biotechnology Academy, Isfahan, Iran
Abstract:
Acute lymphoblastic leukemia (ALL) is the most common cancer between children and the most frequent cause of death from cancer before 20 years of age. Leukemia can make many problems for families and society and this is a good reason for recognizing biomarkers. Different genetics and environment risk factors can cause this cancer. Therefor for finding more bioinformatic information we used NCBI,miRbase, miRWALK2.0, Target scan, DAVID database and KEGG pathway.HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis and apoptosis.This gene is a prognostic biomarker and involved in the treatment of leukemia.According to bioinformatics studies, has-miR-143- 3p is predicted to target the HIF-1α gene in the angiogenesis pathway leading to cancer. And because rs11549465 is located in the coding region of the HIF-1α gene and this miRNA is exactly the same part of the gene, you are likely to target this polymorphism and thereby regulate gene expression in the cancer signaling pathway. In the present project, if according to bioinformatics predictions, the binding site of this microRNA is precisely the rs11549465 polymorphism allele and also the mutant allele, and due to the oncogenic role of HIF-1α gene and negative regulatory function of microRNAs, The expression of has-miR-143-3p is expected to decrease and consequently increase the expression of the target gene,, For this reason, it is predicted that has-miR-143-3p has less binding ability to the mutant allele (T) rs11549465 than the dominant (C) allele of this SNP and therefore the mutant allele may be dangerous and cause its gene expression to be increased and Lead cancer.
Keyword: Acute lymphoblastic leukemia,miRNA,SNP, rs11549465, has-miR-143-3p,HIF-1α
170 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 41557
Molecular mechanisms of the anti-inflammatory effects of thymus vulgaris L. in COVID-19 patients
Amir Abbas Shiravi1, Ali Nadi2, Zahra Mohammadi3, Amin Aslani1, Mehrdad Zeinalian1,4* 1. Department of Genetics and Molecular biology, School of Medicine, Isfahan University of Medical sciences, Isfahan, Iran 2. School of Nutrition and Food Sciences, Isfahan University of Medical sciences, Isfahan, Iran 3. Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran 4. Ala Cancer Control and Prevention Center, Isfahan, Iran
Introduction: A worldwide pandemic infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of current deadly disease entitled COVID-19. Many attempts have been made to find a solution to treat the disease, but no definitive cure has been found, yet. Thymus vulgaris L. is a plant with a long history in traditional medicine that has antimicrobial, antiseptic, and antiviral properties. current study is about some molecular mechanisms of the potential effects of thymus vulgaris against COVID- 19 side-effect. Description: SARS-CoV-2 leads to damage in the body organs by increasing oxidative stress, inflammation, and disturbance in RAS. Thymol and Carvacrol are two important biological components of thyme. Thymol exhibits several biological effects such as anti- inflammatory, antioxidant, immunomodulatory, and free radical scavenging properties. Carvacrol also exhibits antioxidant activities, antimicrobial and anti-carcinogenic effects. On the other hand, interaction of the SARS-CoV-2 and ACE2, its possible receptor, leads to decrease of ACE2. Moreover, studies have illustrated that in COVID-19 patients, serum AngII level is higher than healthy people which can upregulate the expression of inflammatory cytokines through the activation of AT1R. NF-kB, which is down-regulated by thyme, plays a vital role in the regulation of several pro-inflammatory genes. Thyme could suppress TNF-α and IL-6 and other inflammatory cytokines. It also enhances the anti-inflammatory cytokines like TGF-β and IL-10. Thyme extract acts also as an inhibitor of cytokines IL-1-beta and IL-8, at both mRNA and protein levels. Discussion and conclusion: Finally, we can conclude that thyme has anti-inflammatory, antioxidant, and immunomodulatory properties and reduces side effects in COVID-19 patients. Thymol and Carvacrol suppress the negative effects of the renin-angiotensin system in COVID-19 patients. Moreover, they decrease pro-inflammatory cytokines. Thus, Thyme, as an inhibitory agent of pro-inflammatory cytokines, can be considered in the
therapeutic approach of the patients with COVID-19.
171
Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 41629
Studying the effects of essential oil and extract of Teucrium Polium on cell proliferation of Gastric Adenocarsinoma Cell lines
Pegah Hejazi1, Malek Hossein Asadi2*, Farzane Kargar3, Mojtaba Mortazavi2 1. MSc Student, Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran. 2. Assistant Professor, Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran 3. PhD Student, Department of biotechnology medicine faculty of advanced medical science, Tabriz University of medical science, Tabriz, Iran.
Abstract: Gastric cancer is considered the second death cause, which has been poorly developed in prevention and control therapies. In this regard, detecting a significant treatment can be fruitful. Recent studies have shown that chemical medications affect healthy cells in the patient's body adversely. Therefore, new therapies with higher efficacy and fewer side effects, such as medicinal herbs, are considered by researchers. In fact, secondary metabolites in some plants are known as a substantial cure in antioxidant and anticancer treatments. Previous studies have shown that Teucrium polium extract inhibits the proliferation of prostate cancer cells. On this subject, the effects of alcoholic and methanolic extracts of T. polium plant, which we obtained from the mountains around Yasuj city, were evaluated as a potential treatment for human Gastric cancer cure via this research. The results show that ethanolic, methanolic and essential oil of T. Polium have had the cytotoxic effect on AGS cells. According to the results of MTT and flow cytometry, it appears that the anticancer compounds in the T. polium extract likely regulate the cell cycle, stimulate the Tumor suppressor proteins, inhibit cells to grow, and cause cancerous cells to death as well. Keywords: Gastric cancer, Teucrium Polium, MTT, flow cytometry, essential oil, herbal medicine
172 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 42964
Bioinformatics assessment of PPARγ role in regulation of gene networks expression involved in congenital heart disease
Maryam Baharlooie1, Kamran Ghaedi1, 3, Maryam Peymani2, 3, Mohamad Hossein Nasr-Esfahani3 1. Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran 2. Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran 3. Department of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
Introduction & Objectives: Congenital heart diseases impose a huge burden on societies, but the mechanisms for their pathogenesis and control in gene expression context are not well understood. The role of PPARγ transcription factor in the differentiation and development of many tissues and cardiovascular disease has been identified. But the mechanism of its effect is not known. Materials & Methods: In this study, microarray data on congenital heart disease at the GEO database were analyzed by R software and genes with significant differential expression (P<0.05) were candidate for investigation of presence of PPARγ response elements known as PPRE. Protein interactions of these genes were analyzed in Cytoscape software. Also, 20 genes with the highest average interaction score (minimum confidence = 0.4) were predicted in the constructed network and enrichment of these genes was studied at Gene Ontology database. Results: Of the 1802 genes that showed expression alteration in congenital heart disease, 8 genes including PGK1, PKM, BCL2, GHITM, VEGFA, SLC9A1, CAT and TNFSF10 had PPRE elements in their promoter and their interaction with genes involved in biological processes including Regulation of cell death, Regulation of metabolic process, Anatomical structure development and Cell differentiation was identified. Conclusion: The genes found in this study are associated with processes related to development, differentiation, regulation of cell death and regulation of cellular metabolic processes. These processes play a key role in the development of congenital heart disease. On the other hand, the expression of these genes is potential of being regulated by the PPARγ. So PPARγ can be proposed as a potential factor in regulating key processes of congenital heart disease and identifying its potential value in Diagnosis and treatment of these abnormalities, especially in the embryonic period, is suggested for experimental
study. Keywords: Congenital heart disease, PPARγ, PPRE, Gene Ontology, expression network
173
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 43139
Detection of novel mitochondrial mutations in cytochrome C oxidase subunit 1 (COX1) in patients with familial adenomatous polyposis (FAP)
Elham Afkhami*, Mohammad Mehdi Heidari, Mehri Khatami, F Ghadamyari, S Diantpour
Department of Biology, Faculty of science, Yazd University, Yazd, Iran. Background: Familial Adenomatous Polyposis (FAP) is an Autosomal dominant inherited disorder and a rare form of colorectal cancer (CRC) that represents the most common gastrointestinal polyposis syndrome. Generally, cancers start to develop a decade after the appearance of the polyps. It manifests equally in both sexes, and incidence of this disease is in the second decade of life. Cytochrome c oxidase subunit I (COX1) is one of three mitochondrial DNA (mtDNA) encoded subunits (MT-CO1, MT-CO2, MT-CO3) of respiratory complex IV. Alteration of the electron transport components by mutations in mtDNA may compromise the normal electron flow. This could lead to an increase of bifurcation and generation of super oxidase radicals and increase oxidative stress in various types of cancer cells. Methods: In this study, 21 Iranian patients and 20 healthy controls were investigated for presence of the mutations in mitochondrial coding gene (COXI) by PCR and sequencing analysis. FAP symptoms in our patients diagnosed by specialists from Khatamolanbia Hospital of Tehran, Iran. Then, the analysis of pathogenicity of the MT- COXI mutations was accomplished by the human mitochondrial genome database (Mitomap), PolyPhen, Sipred, Expacy and SIFT databases. Results: Our results showed that one patient has a heteroplasmic mutation which is located in MT- COXI gene, (C6041G) that cause change in amino acid (N → K) and according to the results of the in-silico analysis, it was assessed as pathogenic mutation. Also, this mutation is novel and has not previously been reported in any other disease. Furthermore, one homoplasmic variant (16362T>C) in COXI gene was also identified. The Bioinformatics’ predictions show that these mutations probably disturb the process of gene expression. Conclusion: This study is the most comprehensive study in the Iran and the results of this study can be used for genetic counseling and prenatal diagnosis and suggest that mutations in mitochondrial coding genes might lead to the production of defective proteins in the respiratory chains, so potentially lead to CRC in Iranian subjects. The prediction of the functional consequences of mutations, damaging effects and pathogenicity scores of missense and nonsense mutations performed by polymorphism phenotyping v2 (PolyPhen-2), scale-invariant feature transform (SIFT), I-Mutant online service and ENTPRISE-X. Finally to visualizing the 3D shapes and binding changes in the protein PyMol software was used. Keywords: Familial adenomatous polyposis, Mitochondria, MT- COXI gene, Mutations.
174
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 43643
Construction of Multi-Component Scaffold Consisting of DNA Building Blocks and Aptamer Base on DNA Self-Assembly
Sajad Rajabpour1, Hadi Ravan1, Mehrnaz Mehrabipour1, 1. Department of Biology, Shahid Bahonar University, Kerman, Iran
Abstract: Many studies have focused on DNA nanostructures for drug delivery; however, they have encountered many challenges and drawbacks including; destruction by nuclease, cell absorption problem and not being able for epithelial transmission. In spite of these problems, there are some techniques that can help us to address this problem. Therefore, we designed a tetrahedron which easily can be made by DNA self-assembly which has some beneficial characteristics like biocompatibility, ability for cell uptake and being programmable. Besides, in order to have higher rate of stability, we added four aptamer strands on the designed tetrahedron that can strengthen its functionality. Keywords: Dynamic DNA Nanotechnology, Self-Assembly, Tetrahedron
175 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 44358
The effect of licorice extract on prostate volume and its clinical symptoms in patients with benign prostatic hyperplasia (BPH)
Azam taheri1*, Farhad Tadayyon2, Mohammad Fazilati3 1- Payamnoor University of Isfahan, Isfahan, Iran 2- Departman of urology Isfahan University of Medical Sciences, Isfahan, Iran 3- Isfahan University of Sciences, Isfahan, Iran
Background: BPH (Benign Prostatic Hyperplasia) occurs in men over the age of 40, in which the prostate becomes so large that it is difficult to urinate. The symptoms of this disease are annoying for the patients and the treatment process should be done before the symptoms of the disease get worse and lead to surgery. Herbal medicines are effective in treating this disease and have very few side effects. In this study, the effect of licorice was investigated in the treatment of this disease. Materials and methods: The licorice extract was extracted in the laboratory as the main drug filled in 250 mg capsules and the placebo was pure chickpea flour filled in 250 mg capsules because it has no effect on this disease and does not cause side effects, both were given to patients who were divided into 2 groups of 30, to use two capsules per day for three months. Ultrasound and filling the AUA questionnaire were done by patients before and after taking the drugs. Results: In the group that took the drug, the prostate volume decreased and the results of the treatment of disease were evident, but in the group that took the placebo, a small increase in prostate volume and the usual symptoms of the disease was observed. The results of the AUA questionnaire also showed a clear improvement in patients' condition. Conclusion: Licorice extract by inhibiting the enzyme, 17hydroxy steroid dehydrogenase, prevents the conversion of 17hydroxyprogesterone to androsterone ion and so, testosterone and ultimately active testosterone will not be produced and the production of active testosterone, which causes hyperplasia, will be inhibited. Keywords: benign prostatic hyperplasia, licorice Extract, testosterone, prostate volume
176 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 44638
Epi-miRNAs: A Subclass of MiRNAs in Cancer
Somayeh Zeinalzadeh1, Behzad Narimani Eslami2*, Sajad Akbarnejad2
1. Department of Genetic Engineering, Faculty of Basic Sciences, Islamic Azad University, Kazerun, Iran 2. Student Research Committee, Factually of Medicine, AJA University of Medical Sciences, Tehran, Iran Introduction: Cancer is due to the accumulation of genetics and epigenetics changes in cells. Aberrant methylation of CpG rich sequences as an epigenetic change has been observed in almost every human cancer. A large part of malignancies causing genes regulate with microRNAs (miRNAs) that bind to 3΄ untranslated region (3΄UTR) of mRNAs. A subclass of miRNAs, named “epi-miRNAs”, which target epigenetic regulators such as DNA methyltransferases (DNMTs), HDACs or components of the polycomb repressor complexes, have proven to contribute to the epigenetic cellular landscape and represent novel tools to revert aberrant epigenetic alterations commonly found in neoplasm . Description: Comprehensive survey performed by searching in pathway analysis database such as KEGG and STRING to provide the list of genes that were involved in epigenetic especially in cancer. In second step we checked miRNA databases such as miRanda, TargetScan and miRTarBase to find miRNAs that target these genes. Finally, with perfect literature review multiple literature databases such as Google Scholar, Web of Science, Scopus and PubMed databases for reach to articles that reported expression of these miRNAs in cancers . Discussion and conclusion: Our survey demonstrates that DNA methylation is regulated by several genes including: family of DNMTs, HDAC and HAT genes family. In other hand we list several miRNAs that targeted these genes with two categories: predicted and validated. For example, some of validated miRNAs including: miR-148a, miR152, miR- 222 and miR-29 directly target the mRNA for DNMT1 at 3’UTR. In details miR-148a and miR-152 targeted DNMT1 and miR-29 directly targets DNMT3a and DNMT3b. another miRNAs including miR-101, miR-290 and …, that targeting EZH2, RBL2 and other genes that involve in epigenetic regulation. Finally, regard to importance of epigenetic change in cancers, we propose that in future research detection of these miRNAs, in tissue also in serum/plasma, may enhance the sensitivity and specificity of diagnostic and prognostic tests for early-stage many type of cancer. Keywords: Epi-miRNA, Cancer, epigenetic
177
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 45717
A novel dual thermo‑ and pH‑responsive supermagnetic iron oxide nanocomposite hydrogel as controlled release of anticancer drug
Nastaran Dianatnejad*, Masoome Babaei, Farzaneh Kashef, Mohammad Kazem Parastesh
Department of paramedics, University of Danesh Alborz,Qazvin, iran
Abstract: The new generation of therapeutic systems for cancers focusses on the concept of targeting and slowing the release of drugs to long-term the therapeutic efficiency and reduces the side effects which mainly originate from the high dosage of the drug in the blood. Today, there are many reports on the use of magnetic high surface area nanoparticles with the ability to respond to pH changes of environment. The use of these nanoparticles enables the targeted transfer of the drug to the target tissue. In this paper, a novel iron oxide nanocomposite hydrogel was prepared by simultaneous formation of superparamagnetic iron oxide nanoparticles and cross-linking of poly(acrylamid) grafted onto salep polysaccharide. The prepared optimized hydrogel was characterized by Fourier transform infrared spectroscop, scanning electron microscopy with energy-dispersive X-ray analysis, and transmission electron microscopy. Moreover, swelling capacity of the obtained hydrogel was measured at diferent temperatures, diferent pHs, absorption under load, and magnetic feld to assess the sensitivity of ION–PAAm-g-Salep hydrogel. This hydrogel was also examined as a controlled drug delivery system. Doxorubicin (as model drugs) release was investigated at diferent pHs, diferent temperatures, and magnetic feld. The release curves were nicely ftted by the Korsmeyer–Peppas equation, and the release is controlled by polymer relaxation process. Keywords: Magnetic iron oxide nanoparticles, Poly(acrylic amid),Salep-polysaccharide, Drug delivery
178 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 45781
Mutation of Caspase Cleavage Site in XIAP and its Effect on Interaction
R. Hamidi1, F. Ataei*1, S. Hosseinkhani1 1. Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Apoptosis as a form of programmed cell death includes a proteolytic cascade that dismantles damaged or unwanted cells. At the heart of the apoptosis pathway are caspases with clear role for cleaving their substrates. In general, there are two ways to stop caspases activity in both extrinsic and intrinsic pathways of apoptosis: first, remove it from the cell by proteasome degradation machinery; second, direct inhibition of its enzymatic activity. There is evidence that inhibitor of apoptosis protein (IAP) family members can terminate caspases activity at both ways. XIAP as a famous IAP binds amino-terminal of the small subunit of monomeric caspase-9 in apoptosome complex through its BIR3 and also binds caspase-3 and caspase-7 through its linker-BIR2 resulting in obliterating their enzymatic activity and inhibition of cell apoptosis. In this study, an effect of a mutant form of XIAP on interaction with caspase-9 has been investigated. METHODS: This investigation is based on protein complementation assay approach using split-luciferase to detect XIAP and caspase-9 interaction. In this method, caspase-9 and XIAP proteins have been fused to each amino and carboxy terminal fragments of firefly luciferase and a mutation was introduced on XIAP by site-directed mutagenesis. The co- transfected cells of these constructs have been assessed for luciferase activity with and without treatment using apoptosis-inducing agents. Protein expression level was also confirmed with immunoblotting. RESULT AND CONCLUSION: Split luciferase-activity showed that native form of XIAP interacts with caspase-9, however this interaction is affected by caspase-3 mediated cleavage of XIAP. Mutagenesis of caspase cleavage site on XIAP showed this mutant has inhibitory effect on caspase-3 activation but XIAP/caspase-9 interaction is preserved. Keywords: XIAP, Caspase, Mutation.
179 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 48987
Comparison of Two Methods of Ice-COLD HRM-PCR and Full-COLD HRM-PCR in Detection of E542K Mutation in PIK3CA gene in Breast Cancer
Sara Feizbakhshan1,2,3*, Farinaz Khosravian2,3, Mansoor Salehi2,3,4, Mohammad Kazemi,2,3,4, Ahmad Hamta1 1. Department of Biology, Arak University, Arak, Iran 2. Cellular, Molecular and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran 3. Medical Genetics Research Center of Genome,Isfahan University of Medical Sciences, Isfahan, Iran 4. Department of Genetics and Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran
Introduction & Objectives: The most common cancer through women is breast cancer. Genetic mutations play a major role in the development of breast cancer. About 5% to 10% of cases of breast cancer are inherited, and most cases are reported by somatic mutations. According to the COSMIC database, one of the most important mutations in PIK3CA gene is E542K located in exon 9. Materials & Methods: Co-amplification at lower denaturation temperature PCR (Cold PCR) is a technique that selectively enriches the mutated DNA in the background of healthy DNA. In this method, by using the critical denaturation temperature (TC) temperature in the polymerase chain reaction and choosing the WT-mutant type heteroduplex denaturation, enriches the rate of mutant alleles. The difference between Ice- COLD PCR and Full- COLD PCR is the use of a blocking oligonucleotide in Ice-COLD PCR that prevents healthy DNA strands from replicating. In this study, two methods of COLD-PCR and high-resolution melting (HRM) were combined for detection of mutation E542K in exon 9 of PIK3CA and the two methods Full- COLD PCR-HRM and Ice-COLD PCR-HRM are compared. Results: The ice Cold-PCR method, with selective DNA replication, is capable of enriching a small amount of mutated DNA in a mass of wild type DNA. Cell line experiments resulted in almost 4 fold augment in sensitivity by use of ice-COLD PCR/HRM. Conclusion: The results showed us that both methods have advantages and are useful in different applications .Our results clearly demonstrated that ice-COLD PCR/HRM could detect lower levels of mutations in wild-type background. as a sensitive method; therefore, it can prospectively be used in screening of patients with early-stage breast cancers. Keywords: PIK3CA, Breast cancer, COLD-PCR, Ice COLD-PCR
180 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 49428
Characterization and Development of Skin Allograft Scaffolds as a Biological Dermal Substitute for Wound Healing
1Reza Samanipour,2 Ayda Pourmostafa , 3AmirHossein Tavakoli 1. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 2. Department of research and development Iranian Tissue Product Co, Tehran, Iran. 3. Iranian Tissue Bank & Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Introduction & Objectives: Ideally, skin autograft represents a gold standards treatment; however, the biological origin of skin allografts makes an optimal result for massively infected wound and severely burned patients. The acellular dermal matrix contains collagen and elastin, which contribute tensile strength and elasticity. This scaffold performs as mimicking skin extracellular matrix for re-epithelialization, neovascularization, and fibroblast infiltration. Materials & Methods: Acellular dermis matrix (ADM) is derived from donated human skin supplied under the guidelines of the American Association of Tissue Banks (AATB) in GMP cleanroom of Iranian Tissue Product Co. Donor medical history and the results of serological testing were reviewed by the medical director. Epidermal and dermal cells were removed without damage to essential biochemical and structural components by a mild decellularization protocol, and grafts were lyophilized while maintaining the structure and sterilized by gamma irradiation. The morphology and cell attachment of the ADM studied by the Scanning electron microscope (SEM), structure and functional groups characterized by FT-IR spectroscopy. Cytocompatibility, cells viability was evaluated by MTT assay. Results: The SEM image presents the fibrous and porous structure of the matrix after processing and sterilization, and exhibited a good cell attachment. Furthermore, by analyzing the FTIR spectroscopy, the recovered graft protein backbone was confirmed by the presence of characteristic amide I, amide II and amide III peaks of the protein component and mainly collagen. The MTT assay indicated that cells cultured on ADM proliferated well, and the cell viability in 3 days reported 70%, which shows an increase in cell proliferation as increasing culture period. Conclusion: The characterization studies demonstrate the minimum structural and chemical change in properties of ADM after processing which indicates that it is the most appropriate skin substitute based on cell attachment and in vitro cell viability. Keywords: Acellular dermis matrix, Allograft, sterilization processing.
181 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 49805
Evaluation of the effect of ECM on poly caprolactone/Fibrin hybrid scaffold fabricated by 3D Printing method
Ali Valiani1, Ali Honarvar1*, Batool Hashemibeni1, sho,leh ghaedamini1, Saeed Karbasi2, Mohsen Setayeshmehr2
1. Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 2. Department of Biomaterials, Tissu Engineering and Nanotechnology, School of Advanced Medical Technologies, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract: Retinal degenerative diseases lead to blindness due to poorly regenerative potential of the retina. Recently, cell therapy is more considered for degenerative diseases. Autologous mesenchymal stem cells derived from adipose tissue are a suitable source for this purpose. Therefore, we conducted a stepwise efficient method to differentiate human adipose- derived stem cells (hADSCs) into retinal precursor-like cells in vitro. We compared two differentiation protocols, monolayer and hanging drop cultures. Through the defined medium and 3D hanging drop culture method, we could achieve up to 75%retinal precursor gene expression profile (PAX6, RAX, CHX10, and CRX) from hADSCs. By imitation of in vivo development, for direct conversion of stem cells into retinal cells, the suppression of theBMP, Nodal, and Wnt signaling pathways was carried out by using three small molecules. The hADSCs were primarily differentiated into anterior neuroectodermal cells by expression of OTX2, SIX3, and Β-TUB III and then the differentiated cells were propelled into the retinal cells. According to our data from real-time PCR, RT-PCR, immunocytochemistry, and functional assay, it seems that the hanging drop method improved retinal precursor differentiation yield which these precursor-like cells respond to glutamate neurotransmitter. Regarding the easy accessibility and immunosuppressive properties of hADSCs and more efficient hanging drop method, this study may be useful for future autologous cell therapy of retinal degenerative disorders.
182 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 49941
Retinal Precursor-Like Cells Differentiation of Human Adipose-Derived Stem Cells by Hanging Drop Culture
Hossein Salehi1 and Noushin Amirpour1 1- Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract: Retinal degenerative diseases lead to blindness due to poorly regenerative potential of the retina. Recently, cell therapy is more considered for degenerative diseases. Autologous mesenchymal stem cells derived from adipose tissue are a suitable source for this purpose. Therefore, we conducted a stepwise efficient method to differentiate human adipose- derived stem cells (hADSCs) into retinal precursor-like cells in vitro. We compared two differentiation protocols, monolayer and hanging drop cultures. Through the defined medium and 3D hanging drop culture method, we could achieve up to 75%retinal precursor gene expression profile (PAX6, RAX, CHX10, and CRX) from hADSCs. By imitation of in vivo development, for direct conversion of stem cells into retinal cells, the suppression of theBMP, Nodal, and Wnt signaling pathways was carried out by using three small molecules. The hADSCs were primarily differentiated into anterior neuroectodermal cells by expression of OTX2, SIX3, and Β-TUB III and then the differentiated cells were propelled into the retinal cells. According to our data from real-time PCR, RT-PCR, immunocytochemistry, and functional assay, it seems that the hanging drop method improved retinal precursor differentiation yield which these precursor-like cells respond to glutamate neurotransmitter. Regarding the easy accessibility and immunosuppressive properties of hADSCs and more efficient hanging drop method, this study may be useful for future autologous cell therapy of retinal degenerative disorders.
183 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 51075
Review on the main types of eukaryotic and prokaryotic promoters and a glimpse of promoter engineering
Tavalaei Zahra a, Khanahmad Hossein a
a Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Abstract: One of the most important components of a transcription system is the promoter. They are sites for RNA polymerase acceptance and then provoke initiate of transcription. promoters determine speed of RNA synthesis; therefore, the amount of produced protein is largely due to the nature of the promoter. Generally, there are several types of promoters according to different types of species and gene expression levels include of constitutive promoters, inducible promoters, tissue specific promoters and bidirectional promoters that each one has its own details. Also promoters have the central role to design the novel and distinct expression systems in order to alter gene expression level through creating appropriate expression vectors. Undoubtedly knowing about detailed features and function of different types of promoters can help to choose convenient promoter for specific biological aims such as manufacture of pharmaceutical drugs and antibiotics. In this review we discuss properties of diverse eukaryotic and prokaryotic promoters in terms of function and structural details to provide comprehensive information about them. Also we aim to describe how engineered promoters are synthesized by enumerating several common techniques with review on synthetic promoters features and their importance in producing desirable biological products. Keywords: RNA polymerase, Constitutive promoters, Inducible promoters, Tissue specific promoters, Bidirectional promoters, Synthetic promoters, Eukaryotes, Prokaryotes
184 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 51555
PBMCs: A New Source of Diagnostic and Prognostic Biomarkers
Mostafa Saghi1, Shima Rahimirad2, Nasim Vatandoost2, Sharifeh Khosravi2* 1. AJA Cancer Epidemiology Research and Treatment Center (AJA-CERTC), AJA university of medical sciences, Tehran, Iran. 2. Department of Genetics and Molecular biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Introduction: According to the application in different disease stages, biomarkers can be classified as diagnostic, prognostic, and pharmacological. There are various types of molecular biomarkers that are derived from distinct starting materials. Although many indirect biomarkers are found in blood, their detection remains a challenging issue because of the high degree of fragmentation, minute quantity and a vast amount of nonspecific background. Description: Multiple recent studies that have used PBMCs as a source of biomarkers reveal the alteration of mRNAs/microRNAs (miRNAs) signature and methylation profile in many kinds of disorders; for instance, dysregulation of mRNAs/miRNAs in schizophrenia, diabetes and different types of cancers and change in the methylation status of LINE-1 in neoplasms. The present review points out the sensitivity and specificity of peripheral blood mononuclear cells (PBMCs) as an intact source of biomarkers in a variety of diseases. Conclusion: With a strong probability, PBMCs mimic conditions of some tissues which are in contact with them like the tumor cells, hence providing a non-invasive and suitable source of biomarkers. Keyword: PBMCs, biomarker, diagnosis, prognosis
185 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 51619
Anti-tumor effects of NDV in a female Doberman with lymphoma cancer
Abdolreza Nabinejad*1 1-Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, (AREEO), Karadj, Iran Corresponding authors:[email protected]
Introduction & Objectives:Newcastle disease (ND) is a highly contagious and fatal disease of poultry caused by Newcastle disease virus (NDV) which is classified under the genus Avulavirus within the family paramyxoviridae . NDV is an enveloped, single stranded negative-sense RNA virus whose genome is approximately 15 kb, Its genome has six open reading frames (ORFs) which encode for six major structural proteins, namely, nucleoprotein (NP), phosphoprotein (P), matrix protein (M), fusion protein (F), hemagglutinin-neuraminidase (HN), and the RNA-dependent RNA polymerase (L), The cleavability of the fusion protein precursor (F0) and the presence of a number of basic residues in the fusion protein cleavage site are major determinants for NDV pathogenecity as lentogenic, mesogenic and vellogenic, The apoptosis induction and anti-metastatic of malignant cancer cells by NDV carried out by F0 protein .La Sota strain is a mesogenic NDV and genetically similar to wild NDV, RRQRRF motif isolated from Iranian farms. Materials & Methods: In current report a female 40 moths Doberman dog with some pathognomic signs of lymphoma and positive laboratory tests were candidate for NDV therapy as anti-tumor at 2019 April. The animal treated by supportive and antibiotics treatments and suitable nursing, the lyophilized La Sota NDV strain vial were prepared 6 from Razi Institute (RVSRI) and used in 5 to 10 times of vaccinal dose (10 EIDR50R ) in 5 ml PBS as parenteral injection with interval of 3 days for a period of 15 days. Conclusion: Regarding to laboratory exams and clinical sign the lymphoma were controlled and no any metastatic tissues detected in the studied Doberman. Key words: NDV, Anti tumor, Lymphoma, Dog
186 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 51913
Skin Mast Cell Promotion in Random Skin Flaps in Rats using Bone Marrow Mesenchymal Stem Cells and Amniotic Membrane
Farzaneh Chehelcheraghi1*, Leila Zarei2 1. Department of Anatomical Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran 2. Department of Medical Histology, Lorestan University of Medical Sciences, Khorramabad, Iran Background: Skin flap procedures are employed in plastic surgery, but failure can lead to necrosis of the flap. Studies have used bone marrow mesenchymal stem cells (BM-MSCs) to improve flap viability. BM-MSCs and acellular amniotic membrane (AAM) have been introduced as alternatives. The objective of this study was to evaluate the effect of BM- MSCs and AAM on mast cells of random skin flaps (RSF) in rats. Methods: RSFs (80 × 30 mm) were created on 40 rats that were randomly assigned to one of four groups, including (I) AAM, (II) BM-MSCs, (III) BM-MSCs/AAM, and (IV) saline (control). Transplantation was carried out during the procedure (zero day). Flap necrosis was observed on day 7, and skin samples were collected from the transition line of the flap to evaluate the total number and types of mast cells. The development and the total number of mast cells were related to the development of capillaries. Results: The results of one-way ANOVA indicated that there was no statistically significant difference between the mean numbers of mast cell types for different study groups. However, the difference between the total number of mast cells in the study groups was statistically significant (p = 0.001). Conclusion: The present study suggests that the use of AAM/BM-MSCs can improve the total number of mast cells and accelerate the growth of capillaries at the transient site in RSFs in rats.
187 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 51987
Recent advances on aptamer-based biosensors to detection of prostate specific antigen (PSA)
Fatemeh Farshchi 1,2* 1. Pharmaceutical Analysis research Center, Tabriz University of Medical Sciences, Tabriz, Iran 2. Department of Biochemistry, Higher Education Institute of Rab-Rashid, Tabriz, Iran Corresponding author: [email protected]
Introduction: Prostate cancer is the most widespread cancer in men for new cancer diagnoses and the third important reason of cancer demise in the United States in 2017. Early detection of cancer usually increases the chance of successful treatment and quality of life in cancer patients. The detection of cancer biomarkers in blood or serum samples has developed as a powerful medical tool for early diagnosis, prognosis, and monitoring of treatment response in these patients. Prostate-specific antigen (PSA) is a widely used biomarker in clinical screening for prostate cancer. Description: PSA also replaced as a biomarker in many noncancerous situations, such as inflammation, infection, trauma, and benign prostatic hyperplasia. Moreover, it is used as an ideal biomarker for diagnosis of other prostatic diseases, such as prostatitis and prostatic hyperplasia Recently, biosensors have developed a crucial state of the art technology in laboratory medicine, particularly in point of care testing. Biosensors are analytical devices that employ biological/biochemical reactions for detecting goal analytes and basically consist of a bio-element and a transducer. Aptamers, as a biocatalyst are reproduction single-stranded DNA or RNA molecules which are able of binding to small molecules, proteins and cells with high specificity and affinity. Unique features of aptamers over antibodies include shelf-life, flexibility in labeling, resistant to denaturation, stability and so on, it makes aptamers excellent candidates for biological analysis and biosensors Discussion and conclusion: In this review, our goal is considered about aptamers selected against PSA, after that we make an attempt to describe and compare the performances of recently developed PSA aptasensors with different detection methods. To the best of our information, there is a few review articles about aptamer-based biosensors (aptasensors) and PSA have been published as far as this but in this review we reported the most recent studies in this field.
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 52036
The CRISPR Approach to Molecular Systems Biology and Personalized Medicine
Zahra Baseri1, Mojtaba Mortazavi1*, Amir Savar Dashtaki2* 1. Department of Biotechnology, Institute of Science and High Technology and Environmental Science, Graduate University of Advanced Technology, Kerman, Iran 2. Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies Shiraz University of Medical Sciences Shiraz Iran
Introduction: Commonly employed in biological research, reductionism strives to study the cellular components individually. In contrast, holism aims at examining all the components of the cell together. The importance of the holistic approach to cell biology is better appreciated once we know that the cellular components do not function in isolation and that all the components are interconnected. The task of the systems biology is to examine all the components of a cell together. Recently, CRISPR-based genome-scale investigations have been employed to shed light on the genes that confer resistant on cells or make them prone to different chemotherapeutic drugs (drug vemurafenib used in the treatment of melanoma), infection by viruses (the influenza virus) and so forth. Vemurafenib works against BRAF protein which is constitutively active in melanoma cells. Method: Figuring out which genes are behind resistance and which ones behind susceptibility to Vemurafenib would allow the treatment to be tailored in a patient-specific manner. This is carried out using a CRISPR library called GeCKO which targets one gene per cell. A control group infected by lentiviral particles but not treated with vemurafenib is used as reference point so as to determine which gRNAs are depleted and which ones are overrepresented in cells infected and treated with Vemurafenib. Result: Target melanoma cells become susceptible to vemurafenib when genes making them resistant are perturbed. Therefore, when sequencing NGSs, the reads of gRNAs that act against these genes are reduced compared to the control. Genes making melanoma cells susceptible to Vemurafenib are conversely read in higher numbers as their ablation results in resistance. Therefore, by having a constellation of such genes we can assay each individual patient to see which mutations they harbor so as to tailor their treatment regimen based on their particular mutation index. Keyword: Systems Biology, Genome-wide CRISPR Investigation, Personalized Medicine
189 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 53097
The effects of Microbiota on cancer
Zeinab Abdollahi1, Mehrdad Zeinalian1 1. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Introduction: The interaction between host genetic elements and the environmental factors, impacts on cancer as a complex disease. One of the reciprocal factors is a group of 1014 bacteria, eukaryotes and viruses existing in the gastrointestinal tract and known as the gut microbiota. Although their main role is still unclear, they play crucial roles in many physiological processes, particularly in inflammatory and immune reactions. Recently studies have shown significant microbial differences between cancer and healthy patients. Description: Using Keywords including gut microbiota, cancer microbiome, through PubMed and Google scholar search engines, 78 articles were investigated between 2010 to the end of 2018. The results overviewed in the context of this article. Discussion and conclusion: Gut microbiota stimulate the production of cytokines against potential pathogens by their antigens and metabolic products. Helicobacter pylori, hepatitis B and C viruses, and human papillomaviruses that count as infectious agents, have been recognized as carcinogenic factors in 20% of all cancers. Dysbiosis _changes in gut microbiota profiles_ is associated with several mechanisms that are not explained yet. One of the most important mechanisms that are related to cancer development, is their effects on the secretion of inflammatory cytokines and immune system. Bacterial signals modify immunity response and simultaneously alert immune cells, to hit the right target. The gut microbiome influences the pathogenesis of stomach cancer by Helicobacter pylori, Colorectal Carcinoma by Escherichia coli, and Bladder Carcinoma by Salmonella enterica typhi. It seems that an active strategy to fight cancer is the microbiota as a holistic hub point for cancer development and improve therapeutic approach. It however, must be proven whether gut microbiota can be used as a biomarker to differentiate healthy and tumor patients?
190 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 53369
Study of Antidepressant Drugs Effect on Development of the Brain
Ranjbar Ramesh1, Ahadi Ali mohammad2, Zamanzade Zahra1*
1. Department of Biotechnology, faculty of Biological Sciencen and Technology, Shahid Ashrafi Esfahani University, Isfahan, Iran 2. Department of Genetics, Faculty of Science, Shahrekord University, Shahrekord, Iran Introduction: Depression, a mood disorder that causes a persistent feeling of sadness and loss of interest, is the most common mood disorder in the general population. This condition occurs twice as often in women as in men. The initial onset of depression peaks during a woman reproductive years. About 7% of pregnant women experience depression during pregnancy. Mothers using antidepressants during pregnancy may cause anxiety disorders in their children later in life because the drugs can interfere with the normal development of the fetal brain. Description: Human brain development is a protracted process that start in the third gestational week with the differentiation of the neural progenitor cells and continued for a few years after birth the brain continues to grow. The processes that guide brain development involve the ongoing interplay of genetic and environmental factors, like proper nutrition starting pregnancy, exposure to toxins or infections and the child experiences with other people and world. Brain tissue is characterized by high level of gene expressions and numerous anatomical changes during fetal and infancy development. Antidepressants have a similar mechanism of function and effects on the brain and may cause changes in brain structure. Some examples of antidepressant drugs are citalopram, fluoxetine, sertralin. Discussion and conclusion: Antidepressant drugs affects gene expression and brain development. Researches showed that mutation on genes that involved in neurogenesis like MCPH1, BDNF, ZNF335 or genes that have a role in Synaptogenesis such as SHANK3 can cause aberrant brain development. Mutation in centrosomal genes (CEP135, CEP152, CENPJ), tubbulin related genes (TUBB5,TUBA1,TUBB2B) and protein kinesin related genes (KIF14, KIF11) influences brain development and cause genetic brain disorders like primary microcephaly (MSPH), macrocephaly and autism.
191 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 53414
Circular RNAs as promising tools for cancer therapy
Homa Mollaei*, Maryam Moudi, Mahboubeh sadat Hosseinzadeh Department of Biology, Faculty of Sciences, University of Birjand, Birjand, Iran *Corresponding author E-mail: [email protected]
Abstract: Introduction: Recent progresses in deep sequencing technologies led to underlying more complexities of the human transcriptome. Circular RNAs (circRNAs) are a new group of regulatory non-coding RNAs that arise from back-splicing process with covalently closed ends. Up to now, different functions were proposed for circRNAs include detaching proteins from their original sub-cellular localization, regulating parental gene expression, RNA-protein interactions and serving as miRNA sponges. Description: In addition, many of the circRNAs have been involved in the pathogenesis of multi-factorial diseases, such as atherosclerosis, neural disorders and lots of cancers. As described above, deregulation of circRNAs may influence cancer- related processes such as proliferative signaling pathways, epithelial-to-mesenchymal transition, angiogenesis, apoptosis and drug resistance and play role in the progression of cancer. So, it would be possible to apply these novel regulators for cancer therapeutic targets through circrNA- miRNA- mRNA network regulating. A probable therapeutic manner may be to target the unique back-splice junction of oncogenic circRNAs by exogenous delivery of complementary small interfering RNA (si RNA). Also, to induce the expression of tumor- suppressor circRNA, exogenous expression can be applied through extracellular vesicles (EVs), such as exosomes and nanoparticles. On the other hands, high cellular stability and capacity of circRNA to sponge miRNA and proteins let us to serve circRNA as a proper vehicle for delivery of therapeutics. Up to now, several studies assessed these approaches to suppress tumor cells of gastric cancer, leukemia, thyroid cancer, bladder cancer, lung cancer and hepatocellular carcinoma. Discussion and conclusion: So far, circRNA therapy is in the beginning and distinct correlation between many differentially expressed circRNAs and tumorigenesis not fully understood. Furthermore, there has been no preclinical evidence where circRNAs alone have been used as targets or therapeutic vectors to treat cancers. So, deeper understanding of circRNA biology is required for future perspectives in terms of molecular therapy of cancer. Keywords: circRNAs, miRNA sponges, cancer therapy
192
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 53632
Tracking of stem cells
Mahdis Ameri ¹*, Dr. Rahmatollah Tavakoli 2, Dr. Behzad Nourian 3
Department of Medical Engineering, Faculty of Engineering, Maziar High Education Institute, Royan, Iran Introduction: A non-invasive method to track stem cells implanted is very important for stem cell transplantation in both animal experiments and clinical applications. With high sensitivity and specificity of imaging of many kinds of molecular of cells, nuclear medicine imaging technique should be able to monitor stem cells transplanted in vivo. Basing on presentation of recent developments of radionuclide imaging and stem cells investigations, possibilities of in vivo tracking of stem cells with various methods are discussed respectively. Receptor imaging maybe firstly utilized to solve the problem for its simplicity but with low stability and universality. Description: In the context of this overview, 24 articles were used between the years 2010 through the end of 2018 using the Keywords of PubMed, Google scholar search engines. Discussion and conclusion: Several preclinical and clinical trials have shown that stem cell therapy is safe and feasible and has the ability to promote cells functional recovery. However, the precise mechanisms underlying the benefits of stem cell transplantation have not yet been fully elucidated. To achieve optimal therapeutic effects and enhance our understanding of the mechanisms by which stem cells promote functional recovery in disorders, it is essential that we develop noninvasive, reproducible, and quantitative in vivo imaging approaches to track stem cell fate. Additionally, the combination of different labeling agents facilitates better and long-term stem cell tracking in vivo with appropriate safety and feasibility. Each imaging modality has advantages and disadvantages, and the combined use of different imaging modalities strengthens their respective advantages, allowing us to gain a better understanding of the homing, distribution, and differentiation of implanted cells in vivo.
193 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 53924
From antimicrobial peptide to anticancer peptide
Azadeh Teimury*, Zahra Rezvani
Department of Cell and Molecular Biology, Faculty of Chemistry, University of Kashan, Kashan, I.R. Iran
Introduction: The term antimicrobial peptides (AMPs) is used to describe small proteins that can kill or inhibit the growth of various microorganisms. These peptides have different origins such as insects, amphibians, mammals, fungi, etc. and have various activities such as antibacterial, antifungal, antiparasitic, anti-inflammatory, anti-cancer, etc. Their number has been steadily increasing since the 1980s. Description: Due to the increasing incidence of cancer worldwide, researchers are looking to replace biological treatments with chemotherapy, because such treatments reduce side effects in patients. In this review, we focus on peptides with anti-cancer activity and based on various studies and researches, first descriptions about these anti-cancer peptides, their origin and mechanism of action are described. We will also review the possibility of developing different drugs and treatments based on these peptides, vaccines and peptide hormones, and the advantages of using them compared to other treatments. Discussion and conclusion: Based on various studies and researches, it has been found that these peptides show a high level of specificity against cancer cells and much higher anti-cancer activity than chemotherapy drugs. Not all antimicrobial peptides have anticancer properties, but the properties that make peptides targets for anti-cancer therapies include differences such as net charge, hydrophobic and amphipathic properties, their secondary structure in membranes, their sequence length, and so on. In the future, biological anticancer therapies will replace therapies with side effects such as chemotherapy.
194 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 54450
Genetic variation in circadian rhythm gene CLOCK with susceptibility to multiple sclerosis
Maryam Saneipour1, Abbas Moridnia1* 1- Department of Genetics and Molecular Biology, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran
Introduction: Multiple Sclerosis (MS) is the most common disabling neurological disorder of young adults that cause inflammatory demyelinating disease of the central nervous system. Evidence from epidemiological studies indicates the prevalence of MS varies with geographic latitude, increasing with distance from the equator. The factors such as fluctuations in temperature day, length and light intensity during different seasons sustain the human circadian rhythms. Interior circadian rhythms are regulated by circadian rhythm genes. The discordance of circadian rhythms is associated with numerous disorders such as neurodegenerative diseases. The aim of this study was to determine a possibility of associating the rs6811520 T>C polymorphism in CLOCK gene with MS. Materials and Methods: 55 patients with MS referred to Ayatollah Kashani Hospital in Isfahan and 60 healthy individuals as control group was selected. DNA was extracted from whole blood and then all subjects were genotyped for the rs6811520 polymorphism in the CLOCK gene by high-resolution melting (HRM) real-time PCR technique. The HRM results confirmed by PCR-Sequencing. Statistical analyses were done with version 20 of SPSS software. Results: Our finding displayed a statistically significant difference in genotype and allele frequency between two groups concerning rs6811520 (P = 0.027, for C allele) polymorphism within the CLOCK gene. Conclusion: The results presented that CLOCK genetic polymorphism are associated with increased risk of MS in Iranian population. However, functional studies are required to explain how this SNP contributes to MS pathogenesis. Keywords: Multiple Sclerosis, CLOCK gene, Polymorphism
195 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 54755
Effect of Glaucium Flavum Extract on Bax and Bcl2 Genes Expression in HT- 29 Cancer Cells
Sima Orouei1*, Ebrahim Rahmani Moghdam2, Hossein Bordbar2,3, Sedigheh Saberifar4, Vahideh Zarrin5
1. MSc student, Department of Genetic Science, Medical Science Branch, Islamic Azad University, Tehran, Iran 2. Department of Anatomical sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. 3. Histomorphometry and stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 4. Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran. 5. Laboratory for Stem Cell Research, Shiraz University of Medical Sciences, Shiraz, Iran.
Introduction and Objectives: Cancer is still considered an issue in society; each year, 2.8 people die from this condition; therefore, the discovery or design of antineoplastic agents with little or no toxicity is necessary. Medicinal herbs are widely used today to treat cancer. The present study aimed at investigating the effect of Glaucium flavum extract on apoptosis induction in HT-29 cell line. Material and Methods: Different concentrations of Glaucium flavum extract (25, 50, 100, and 200 μg/ml) were added to HT-29 cells in the MTT assay. The metabolic activities of the treated cells were studied for 5 hours according to the ODs read by the Elisa-reader. QRT-PCR technique was used to evaluate the expression of Bax, Bcl2 gene. The statistical analysis carried out using SPSS software and One-way-ANOVA test. Results: Analysis of QRT-PCR data showed that the concentration of 50 (μg/ml) as IC50 during 4-hour treatment could increase Bax gene expression and decrease expression of Bcl2. Conclusion: This extract induces apoptosis and cell death as a result of the effects of its alkaloidal compounds. Keywords: Glaucium flavum, Beclin1, HT-29, Autophagy.
196 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 55609
Insight to nano-curcumin interaction with alpha crystallin: spectroscopy and molecular docking study
Mohammad hossein Shabahang1, Sattar Khashkhashi Moghadam1, Jamshidkhan Chamani*1 1. Department of biology, Faculty of Sciences, Mashhad branch, Islamic azad university, Mashhad, Iran [email protected], [email protected], [email protected]
Introduction: Alpha Crystallin is a major lens protein, comprising up to 40% of total lens proteins, where its structural function is to assist in maintaining the proper refractive index in the lens. Curcumin was identified as the principle biologically therapeutic compound of turmeric and was found to possess a wide range of beneficial pharmacological properties including anti-inflammatory, anti-oxidant, anti-microbial and anti-cancer activities. In this study we reveal the nano curcumin treatments features upon interaction with alpha crystallin the by using the biophysical method and molecular docking. Materials and methods: The measurement of fluorescence signals provides a sensitive method for monitoring the biochemical environment of a fluorophore and can give us important information about the molecular level of binding of small molecular substances to the protein, such as the binding mechanism, binding constants, and intermolecular distances.Synchronous fluorescence spectra provide information about changes in the molecular microenvironment in the vicinity of functional fluorophores and are used to investigate the conformational changes of proteins. Molecular docking was used to predict the binding site of nano curcumin to the protein by MOE 2015 software. Results and conclusion: Decreasing in the fluorescence intensity upon increasing the concentration of nano curcumin proved that the interaction happened and complex forming. Synchronous spectra showed that the microenvironment of the TRP and TRY for the protein has changed which induced complex forming. Molecular docking indicated that the 28VAL, 45GLN and 22SER were the most sensitive residue in this interaction Conclusion: In this manuscript we determined the binding mechanism and binding behavior of nano curcumin with the alpha crystallin through biophysical and molecular modeling methods. Keywords: Nano curcumin, alpha crystallin , spectroscopy , molecular docking
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 55702
Nutrigenomics studies and Evaluation of Irisin level Affected by the Expression of Skeletal Muscle Genes
Alieh abdolrezaie1*, Hashem Nayeri1, Ali Mohammad Ahadi2 1. * Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran. 2. Department of Genetic, School of Science, Shahr-e-kord University, Shahr-e-Kord, Iran
Introduction: The point view of nutrigenomics, foods have signals that can affect metabolic pathways, control of homeostasis, gene expression, and protein production. Irisin production is a metabolic pathway that results from the proteolytic breakdown of the FNDC5 (Fibronectin type III domain containing 5) which is secreted by the skeletal muscle as an exercise hormone during exercise and is dependent on PGC-1 gene expression. The aim of this study is to review the effects of diet on the expression of some genes and the production of skeletal muscle proteins. Description: In studies effects of diets on skeletal muscle genes expression, Rats were fed with high-carbohydrate diet (HCD), high-fat diet (HFD), and high-protein diet (HPD). They were evaluated of lipid profile, FNDC5 and PGC-1 expression in muscle and protein levels of Irisin. The results showed that HFD and HCD were associated with decreased of FNDC5, PGC-1 and Irisin level. HPD prevented reductions of FNDC5, PGC-1 expression and Irisin levels, compared to the control group. although no differences were observed for total cholesterol and HDL levels. Discussion and conclusion: The molecular structure of a food can have important effects on gene expression, protein, and metabolism and enable specific signaling pathways. so small changes in structure of food have a profound effect on the sensitive pathways that are activated. In conclusion, it was found that the HPD type of diet can effective on mRNA of muscle cells and change FNDC5, PGC-1 expression and Irisin levels. Therefore, HPD seems to be the most appropriate diet to achieve high levels of irisin and thermogenesis and today, using the HPD diet is a good strategy for treat obesity and as a protein supplements in athletes diet it improve their performance.
198 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 55861
SiRNA Bioinformatics Design Against mRNA of N, NSP12, NSP16, NSP5 Genes to Prevent mRNA Translation and Sars_cov_2 Virus Replication
Hanieh Mirzaei bachelor of nursing _ Islamic Azad University of Khorasgan , Isfahan , lran
Introduction & Objective: The novel coronavirus SARS-CoV2 causes COVID-19, a pandemic threatening millions it can proliferate, unhindered, in primarily infected tissues. Subsequent cell death results in the release of virus particles. interfering RNA (siRNA), sometimes known as short interfering RNA or silencing RNA, is a class of double-stranded non-coding RNA molecules, which is 20-25 base pairs in length. siRNAs can regulate the expression of genes, by a phenomenon known as RNA interference (RNAi). Based on the phenomenon, the siRNA based therapeutics have been developed and implemented for anticancer, antiviral, and genetic diseases, there are many drugs currently being tested which include antiviral (remdesivir, favipiravir, lopinavir, ritonavir, and arbidol), anti- malarial (hydroxychloroquine), and anticancer (interferon-alpha 2b) agents. These drug candidates are undergoing clinical trials, and their efficacy against SARS-CoV-2 has yet to be proven. Under such a situation, siRNA based treatment can provide an effective solution in combating COVID-19 .This is the function of the genes we have selected : NSP5 : Cleaves at 11 sites of (3C-like proteinase) NSP polyprotein ; NSP12 : Copies viral RNA (RNA polymerase) methylation (guanine) ; NSP16 : 5′-cap RNA (2′-O-ribose- methyltransferase_potential antiviral drug target) methylation (adenine) ; N protein : The nucleocapsid (N) protein of coronaviruses is a structural protein that binds directly to viral RNA and providing stability . Method: for design siRNA were used Genlink and siRNA wizard site And based on siRNA design guidelines that include Selected region, siRNA size,Thermodynamic properties , Starting nucleotide and GC content of the siRNA sequence The best siRNA were chosen and then , BLAST against mRNA databases and BLAST against miRNA SEED databases was done. Findings & conclusion: According to the obtained data, siRNA was designed. siRNA is a good choice to fight against viral diseases that can interfere with the replication of the virus, siRNA designed in the next steps will be tested in the laboratory. Keywords: bioinformatic _ siRNA _ sars-cov-2
199
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 56215
Molecular identification of Staphylococcus epidermidis and Staphylococcus aureus in children and adults suspected of sepsis in Rafsanjan city
Elnaz Hosseini Abbasabadi1, Mohammad Javad Soltani Banavandi*2, Shima Mirzaie Parsa3
1. Dep of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran 2. Faculty of Medicine, Kerman Branch, Islamic Azad University, Kerman, Iran 3. Dep of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran Introduction & Objectives: Septicemia due to numerous health risks is considered a medical emergency. bacterial infection is one of the most common infections. some bacterial agents that cause bloodstream infections include Staphylococcus aureus, Pseudomonas aeruginosa, klebsiella pneumoniae, Acinetobacter, Escherichia coli and Enterobacter. Blood infection caused by opportunistic bacteria such as Coagulase-negative staphylococci is one of the major problems of hospitalized and immunosuppressive patients. Since the 1980s, gram-positive microorganisms, especially Staphylococcus aureus, have persisted as a major cause of nosocomial infections. In this study, molecular identification of Staphylococcus epidermidis and Staphylococcus aureus in blood samples suspected of sepsis was performed by PCR method. Materials & Methods: A total of 209 venous blood samples were collected from suspected sepsis patients in Rafsanjan city Medical Laboratories. 100 µl of collected blood was used for culture in blood agar and then in nutrient broth. DNA was extracted by DNG-Plus kit and PCR test was used to detect Staphylococcus epidermidis and Staphylococcus aureus. Results: 55 samples of 209 were grown in liquid culture media. From PCR results, 44 samples were identified as infected with Staphylococcus epidermidis and 11 samples were infected with Staphylococcus aureus. Conclusion: PCR test is a fast and sensitive method that is preferable to conventional laboratory for definitive detection of bacterial sepsis, which is very valuable to both physicians and patients. This study showed that compared to other researches, the prevalence of these bacteria has increased in patients from Rafsanjan city. Keywords: Staphylococcus epidermidis, Staphylococcus aureus, Sepsis, PCR
200
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 56718
Anticancer activity of methanolic Cordia myxa extract on caspase-8 in breast cancer
Nasrin Hadi1,2*, Banafsheh Torkan1,2, Farinaz Khosraviani1,2,3, Mansoor Salehi1,2,3
1. Cellular, Molecular and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran. 2. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. 3. Medical Genetics Research Center of Genome, Isfahan University of Medical Sciences, Isfahan, Iran.
Introduction: Breast cancer is one of the most prevalent females and male cancer in the world. Persistent high mortality rates in breast cancer patients, in spite of the latest advances in diagnosis and therapy, confirms that the necessity of new developments in tumor prognostic [1-2]. natural therapies, such as the use of plant-derived products in cancer treatment, may reduce side effects [8]. Cordia myxa is a plant of Boraginaceae family and has a wide range of antioxidant compounds as well as trace elements including magnesium, copper, iron, zinc, and selenium. In this study the effects of Cordia myxa extract on expression level caspase-8 in breast cancer have been assessed. Materials & methods: In this experimental study, human breast cancer Michigan Cancer Foundation-7 (MCF-7) cell line treated with various concentrations of Cordia myxa extract (0, 10, 100, and 1000 µg/ml). The effects of Cordia myxa extract on cell proliferation were measured by MTT-assay. To investigate the impact of the Cordia myxa extract on the expression level of caspase-8 genes was used quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting was performed to evaluated the level of caspase- 3 protein. Results: Cell proliferation decreased significantly after treatment with methanolic extract of Cordia myxa for 24 and 48 h. It can be assumed the methanolic extract of Cordia myxa has anti-proliferative effects in MCF-7 cell line. Furthermore, Cordia myxa extract led to increase expression of caspase-8. Conclusion: In conclusion, it can be assumed that the methanolic extract of Cordia myxa can be a potential candidate for more investigations in the field of cancer. However, identifying the effective agent of the Cordia myxa extract is necessary for achieving the most successful results. Keywords: Breast cancer, Cordia myxa, Caspase-8
201 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 56746
A review on role and function of REST (restrictive element-1 silencing transcription factor) in neurodegenerative diseases
Amir hossein haji ali asgary 1*, Mahdieh khojasteh 1 1. Undergraduate Student of Cell and Molecular Biology, Faculty of Biological Sciences and Technology, University of Isfahan, Isfahan, Iran
Introduction: REST (restrictive element-1 silencing transcription factor) is a transcription repressor which plays a key role in the expression of neuronal genes. REST not only acts as a master transcriptional regulator for genes involved in neurogenesis and neuronal differentiation, but also contributes to normal aging and synaptic plasticity of the adult brain. Description: REST upregulation induces neuroprotection in the normal aging process by inhibiting genes involved in oxidative stress and -amyloid toxicity. Dysregulation of this repressor or its related epigenetic remodeling may cause neurodegeneration associated with neurodegenerative diseases. In this review, recent𝛽𝛽 findings on the status and function of REST in related neurodegenerative diseases and disorders are reviewed. Discussion and Conclusion: The findings suggest that REST dysfunction at a particular time may cause neurodegenerative disorders and diseases including brain ischemia, Alzheimer's disease, Parkinson's and Huntington's disease. According to the findings, activation of REST in response to ischemia is likely to lead to ineffective epigenetic regulation. REST accumulations are found in the nucleus of some neurons in Huntington’s disease. Also absence of REST, inability to import inside the nucleus and its presence inside the autophagosome are associated with Alzheimer’s and Parkinson’s disease. Therefore, REST may be used as a biomarker for the aforementioned diseases.
202 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 56980
Mechanisms of Ataxia Pathogenesis and Role of Genetic Factors
shafagh sharafi1*, Zahra Razvani1 1. Biotechnology Department, Faculty of Chemistry, University of Kashan, Kashan [email protected]
Abstract: Ataxia means the inability to coordinate movements, which is a cerebral neurological disorder that results from the loss of coordination between muscle movements and affects walking, seeing and talking. Genetic ataxia are very heterogeneous and have different causes. Ataxia can appear as a single symptom with multiple symptoms, indicating a more complex disease. The disease can be temporary or permanent, and may also be caused by environmental factors such as alcohol, trauma or toxic drugs, even some factors such as infection, tumors or vitamin deficiency can also cause the disease, though many ataxia have genetic causes. The prevalence of hereditary ataxia varies with the population, with approximately 1 to 9 out of every 100,000 estimated. One of the types of ataxia is spinocerebllar ataxia (SCA), the autosomal dominant mode of inheritance. These are progressive neurodegenerative diseases that affect the cerebellum but can also affect other areas, including the brainstem. Spinocerebllar ataxia has a variety of types that fall into three groups according to the classification of hardening according to clinical symptoms. Many SCAs are caused by alterations in the repetition of CAG nucleotide sequences that affect polyglutamine gene expression. The 5 main known mechanisms involved in SCA include: Toxic Ribonucleic acid (RNA) gain of function, Mitochondrial dysfunction, Channelopathies, Autophagy, Transcription dysregulation. In this article, it has been attempted to investigate the mechanisms that cause different types of SCAs while investigating ataxia spinocerebllar disease. Keywords: Ataxia, Spinocerebllar, Functional, RNA
203 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 57337
Glaucium Flavum Induction Autophagy in HT-29 Cancer Cells
Sima Orouei1*, Sedigheh Saberifar2, Hossein Bordbar3,4, Ebrahim Rahmani Moghdam4, Vahideh Zarrin5 1. MSc. Student, Department of Genetic Science, Medical Science Branch, Islamic Azad University, Tehran, Iran. Email:[email protected] 2. Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran. 3. Histomorphometry and stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 4. Department of Anatomical sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. 5. Laboratory for Stem Cell Research, Shiraz University of Medical Sciences, Shiraz, Iran.
Introduction & Objectives: Colon cancer is the third most common cancer in the world. In recent years due to the increased prevalence of deaths from cancer and failure of chemotherapy in advanced forms of cancer, there is a need to find new ways to control cancer. One of these methods is the use of herbs. The aim of this study was to investigate the effect of cytotoxicity and autophagy of Glaucium flavum in HT-29 cell line and Beclin1 gene expression analysis. Material and Methods: In this study, the cytotoxicity of the Glaucium flavum extract was evaluated by MTT at concentrations of 25, 50, 100, and 200 µg/ml, and the concentration of 50 µg/ml was used for the determination of IC50. After treatment with IC50, cDNA was synthesized from extracted RNA. The expression of Beclin1 gene relative to the GAPDH gene was evaluated by QRT-PCR. The statistical analysis carried out using SPSS software. Results: The results showed that the concentration of 50 (μg / ml) of Glaucium flavum extract decreased cell viability, and also Beclin1 gene expression in HT-29 cells induces autophagy. Conclusion: Given the cytotoxicity of this extract, and the ability to induce autophagy process in HT-29 cell line, it can be concluded that this extract could be a viable candidate for cancer research. Keywords: Glaucium flavum, Beclin1, HT-29, Autophagy.
204 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 57632
Association of rs4073 with IL-8 activation by transcription factor and its role in angiogenesis, diffusion of immature white blood cell and acute lymphoblastic leukemia by bioinformatics analysis
Marziye Bahrebar 1*, Massoud Houshmand2
1. Department of Genetics, Department of Biology, High Institute nurdanesh, Meymeh, Isfahan, Iran. 2. Medical Genetic Department, National Institute of Genetic Engineering and Biotechnology, Tehran,Iran. * [email protected]
Introduction & Objectives: Acute lymphoblastic leukemia (ALL) is the most common malignancy in children. Recent studies have identified the genetic susceptibility value of SNPs for cancer risk. IL-8 releases immature white blood cells into the blood. Overexpression of IL-8 also causes angiogenesis and metastasis in cancer tumor. Rs4073 is a polymorphism in the IL-8 promoter locus. Polymorphisms at the promoter site affect gene expression by interaction of SNP with transcription factors. The purpose of this study was to investigate the activation of IL-8 by GR transcription factors between individuals with T and A alleles at rs4073, when hsa-miR-let-7a-2-3p inhibits NR3C1 transcription factor. Materials & Methods: Firstly, the transcription factors of the gene was predicted by the PROMO promoter database. The miRWalk2 database was used to identify IL-8-related microRNAs and let-7a-2-3p hsa-miR- was selected. Information of hsa-miR-let-7a-2-3P was obtained from miRbase database. The target gene of this microRNA was identified by using miRNASNPV2 database. The associated signal pathway was identified using the DAVID and KEGG databases. Results: Results from the PROMO promoter databases showed that the presence of the T allele causes binding of the GR-bata transcription factor at this site and this transcription factor doesnt bind in the presence of the A allele. Prediction by the miRNASNP V2 database showed that the target of hsa-miR-let-7a-2-3p is the GR-beta gene (NR3C1). Conclusion: According to the data obtained, it was predicted that hsa-miR-let-7a-2-3p might decrease GR-beta expression and that the Interleukin-8 gene would be less activated by the GR-beta transcription factor. However, despite suppression of transcription factor, it is expected that in pepole with the T allele that have more binding site for the GR transcription factor, the GR transcription factor will activate the IL-8 gene. Finally, studies suggested that increased risk of angiogenesis as well as the release of immature white blood
cells into the bloods would increase IL-8 activation in people with T alleles. Keywords: acute lymphoblastic leukemia, SNP, IL-8, hsa-miR-let-7a-2-3p 205
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 57860
MBD1 Expression and its correlation with Clinicopathological Prognostic Factors
sho,leh ghaedamini1, Mitrasoleimani1* 1. Department of Anatomy and Cell Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Background: MBD1, named methyl-CpG binding protein 1, is a transcriptional repressor and belongs to one member of methyl-CpG binding domain (MBD) family. This protein can regulate both gene expression and methylation level and contribute to the tumor growth in colorectal cancer (CRC). The aim of this study was to examine MBD1expression in patients with CRC and evaluate its potential correlations with clinicopathological factors. Material and Methods: immunohistochemistry was performed to examined the MBD1 protein expression in 60 cases of CRC tissue samples and their corresponding neighboring normal tissues as control samples. In the next step, the correlation of MBD1 with clinicopathological features of patients including sex, gender, stage, location, type of the tumor, grade and metastasis were assessed. Results: The expression of MBD1 Protein downregulate significantly in cancer samples compared to normal control samples. This downregulation increased correspond to metastasis and grade of cancer, however no correlation was seen between the expression of MBD1 and sex, gender, stage, location and type of the tumor. Conclusion: Our data suggest that reduced expression of MBD1 may be contribute to tumor genesis. However, the use of this protein as a prognostic factor requires further research on larger volume samples. Keywords: MBD1, clinicopathological features, Colorectal Cancer
206 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 58253
The effect of Moringa olifera leaf extract on blood glucose in diabetic rats
Shabnam Karimian1, Mohammad Fazilati1, Habibollah Nazem1, Elham Bijad1 1- Department of Medical Informatics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Abstract: Diabetes is a metabolic disorder caused by a defect in insulin secretion or both. Insulin Deficiency Increases Blood Glucose by Disrupting Carbohydrate, Fat, and Protein Metabolism with Diabetes, Vascular or Tissue Damage Causes Severe Complications of Diabetes they give. Thirty male Wistar rats weighing 200 ± 20 were divided into 6 groups of five. Group 1 (control), group 2 (diabetic), group 3 (diabetic + 100 dose mg / kg WB extract), group 4 (diabetic + 200 dose mg / kg WB extract), group 5 (diabetic + 400 dose extract), group 6 (diabetic + drug Metformin at a dose of 100). Diabetes was induced in animals by a single intraperitoneal injection of streptozotocin at a dose of 55 mg / kg WB rat body weight. The mean glucose concentration in the first group was 322.0 mg / dl which was 682.0 in the second group and 166.2, 493.6 and 448.5 in the 3, 4, 5 and 6 treatment groups, respectively and is 572.4. According to analysis of variance, the mean serum glucose concentration in group 1 was significantly different from group 2 (P <0.001). Also, the mean value of this parameter between group 2 and group 3, 4 and 5 had a significant difference at the 0.001 level. In this experiment, we observed an increase in glucose in group 2 compared to group 1 with the effect of Moringa olifera extract on diabetic groups treated with glucose compared to group 2, with a dose of 100 having the greatest effect on reducing glucose. Keywords: Diabetes, Moringa olifera, Extract, Anti-diabetic effect
207 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 59685
Bioanformatic study of effective microRNAs on growth factors of cancer pathway
Negar Hosseini1, Marzieh Abolghasemi1, Fatemeh Kheiri1 1. Genetic Division, Department of Biology, Faculty of Basic Science, Shahrekord Univeristy, Shahrekord, I.R.Iran. *[email protected]
Introduction & Objective: cancer is abnormal proliferation of cells in the body that can lead to death. The crutial role of growth factors in cancer progression has been demonstrated. Using microRNA-related mechanisms is one of the main regulalting genetic processes. These molecules are responsibe for regulating the expression of many genes. The aim of this study is to investigate the microRNAs that target on cancer-promoting growth factor genes and select the most effective microRNAs as a molecular biomarker to cancer diagnosis and treatment. Meterials & Methods: firstly, according to studies taken from articles and NCBI database, the major genes of growth factors were considered and ten genes (EGF, VGFFA, HGF, TGFB, EGFR, ERBB2, ERBB3, ERBB4, IGF1, IGF2) selected as well. Additionally, KEGG database used to consider the cancer pathway and selected genes. Afterwards, miRWalk, miRTarBase and miRDB databases were used to find microRNA that affect candidate genes and select microRNAs targeting more genes. It is worth to mention that each of these databases has different predictive algorithms. The data obtained from all these databases ,for all genes, was recorded in the excel file and ordered by the name of microRNA. Finally, after selecting appropriate microRNAs, in the phenomiR database, the rate of expression and efficacy of microRNAs role in cancer were evaluated. Results: the microRNAs binding to 3’UTR of genes were predicted by using algorithms of above-mentioned databases. Then, from the gained microRNAs, some cases that identified more genes or had more binding sites on a single gene were selected. Conclusion: Considering the bioinformatics studies, has-miR-133a-3p, has-miR-125a-5p and has-miR-205-5p have the highest score therefore they are the most suitable options for further investigation in practical analyses leading to inhibit cell proliferation. Keywords: microRNA, Growth Factors, Biomarker
208 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 59805
Sulfur mustard gas and epigenetic modifications in skin cancer: A Review
Seyed-Naser Emadi (M.D.)1, Mahdi Hadian(M.D.)2, Bahareh Abtahi-Naeini (M.D.)3 1. Skin Research Center, Razi Hospital, Tehran University of Medical Sciences, Tehran, Iran. 2. Medical student research committee, Isfahan University of Medical Science, Isfahan, Iran 3. Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
Introduction: Cutaneous malignancy is caused by the accumulation of genetic and epigenetic alterations, which induce alterations in the expression of genes. The list of environmental factors implicated in cutaneous malignancy include chemical agents isgrowing.Sulfur mustard (SM), a major potent chemical warfare agent in Iran–Iraq warcan cause long-term carcinogenicity especially on skin and lung even years after a single exposure.However, theexact mechanisms of cutaneous cancer development by SM gas are poorly understood.Herein, we review available published data regardingrole of SM as an epigenetic factor in the restoration cutaneous cancer cells. Description: Keratinocyte differentiation is regulated by signal transduction events and transcription factors. In addition, epigenetic regulatory mechanisms have been demonstrated as a regulation of differentiation-related genes.Recent reports indicate that SM convert the normal epigenetic marks during carcinogenesis. After SM gas exposureDNA methylation and histone acetylation or histone di-methylation were documented in human skin sample. SM had only some minor effects on histone modifications. However, a significant and pronounced increase of DNA methylation was detected. So in one hand the main epigenetic mechanisms studied in epidermal cells exposed to SM gas are DNA methylation which finally resulting in changes of cellular phenotypes and in the other hand alterations in DNA methylation have been reported important key in aberrant keratinocyte differentiationand skincancerdevelopment.Some investigation indicate that SM does indeed cause epigenetic modifications that appear to persist over time. Conclusion: There are several epigenetics modificationsby SM in development of skin cancer and even in SM animal models. Since in skin cancer, diverse epigenetic alterations by SM in cancer-related genes occur in the early stages of tumor development, it can be promising targets for the development of novel drugs targeting in feature for provide a linking betweenclinical and molecular findings. Key-words: Mustard Gas; Epigenetic; Skin Neoplasms; DNA methylation
209
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 59845
The investigation of the interaction between piperine and human serum albumin compared with holo transferrin nutural protein by both spectroscopic and synchronous fluorescence technique for cancer prevention
, a Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic𝒂𝒂 Azad University, Mashhad,𝒂𝒂 Iran 𝐒𝐒𝐒𝐒𝐒𝐒𝐒𝐒𝐒𝐒𝐒𝐒 𝐀𝐀𝐀𝐀𝐀𝐀𝐀𝐀𝐀𝐀𝐀𝐀 − 𝐟𝐟𝐟𝐟𝐟𝐟𝐟𝐟𝐟𝐟𝐟𝐟𝐟𝐟𝐟𝐟𝐟𝐟𝐟𝐟𝐟𝐟 𝐉𝐉𝐉𝐉𝐉𝐉𝐉𝐉𝐉𝐉𝐉𝐉𝐉𝐉𝐉𝐉𝐉𝐉𝐉𝐉𝐉𝐉 𝐂𝐂𝐂𝐂𝐂𝐂𝐂𝐂𝐂𝐂𝐂𝐂𝐂𝐂 Abstract: The objective of this study was to effect of piperine on human serum albumin (HSA) and holo transferrin nutural protein(HTF).HSA and HTF have shown potential in the delivery of anticancer drugs into primarily proliferating cancer.Piperine is an active alkaloid with an excellent spectrum of therapeutic activities such as anti-oxidant, anti-inflammatory, immunomodulatory, anti-asthmatic, anti-convulsant, anti-mutagenic, antimycobacterial, anti-amoebic and anti-cancer activities.Since piperine is highly lipophilic,we hypothesize that it could also interact with HSA and HTF through binding displacement and influence their bioavailability.The interaction of piperine with HSA and HTF was investigated in aqueous solution by fluorescence quenching.The quenching mechanism and binding characteristics of piperine with HSA and HTF were obtained from the fluorescence measurement.The alteration in protein conformational stability was determined by fluorescence quenching data.In addition, the alterations of HSA and HTF secondary structure in the presence of piperine were investigated.RLS spectra (Resonance light scattering) were recorded by simultaneously scanning the excitation and emission monochromators from 220 to 660 nm with Δλ=0 nm and synchronous fluorescence from 300 to 600 nm with Δλ=15 nm.The result from synchronouse fluorescence spectroscopy showed that micro-enviroment around Tryptophan and Tyrosine were changed. Keywords: HAS ,HTF ,Piperine ,Spectroscopic ,Synchronous fluorescence ,RLS ,Resonance light scattering
210 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 60074
The role of BACE1-AS and BC200 long non coding RNAs in Alzheimer’s disease
Hannaneh Amirzehni1, *, Asiyeh Jebelli2 1. M.Sc. student of Genetics, Department of Biological Science, Faculty of Basic Science, Higher Education Institute of Rab- Rashid, Tabriz, Iran 2. Assistant professor, Department of Biological Science, Faculty of Basic Science, Higher Education Institute of Rab-Rashid, Tabriz, Iran
Introduction: Alzheimer’s disease (AD) is one of the most common neurodegenerative disease and accounts for >80% of dementia cases in people aged older than 65 years. Long noncoding RNAs (lncRNAs) comprise a subgroup of noncoding RNAs longer than 200 nucleotides, accounting for the largest proportion of the mammalian noncoding transcriptome. LncRNAs are implicated in the development of axonal and dendritic connections, and additionally, in synaptic modulation that is correlated with neural network plasticity and that may also participate in the generation of long-term potentiation that underlies learning and memory. Description: LncRNA BACE1-AS is highly expressed in AD patients and in amyloid precursor protein (APP) transgenic mice. This LncRNA is transcripted by the antisense strand of BACE1 (a protein coding gene) and plays the key role in increasing BACE1 mRNA stability by masking the binding site for miR-485-5p and preventing miRNA- induced translational repression of BACE1 mRNA. This mechanism leads to upregulating of BACE1 protein. BACE1 is a crucial enzyme that cleaves APP and generates amyloid β (Aβ) peptides, which form amyloid plaques on the neurons. In addition to BACE1-AS, the lncRNA BC1 (in mice) and BC200 (in humans) can control the expression of BACE1 mRNA. These lncRNAs modulate gene expression at translational level. BC200 levels is increased in brain regions that are preferentially affected in AD and exhibits abnormal subcellular localization. The primate BC200 is also associated with synapse plasticity. Discussion and conclusion: As dysregulation of lncRNAs involved in AD, these RNAs are desirable candidates as AD biomarkers and could help identify rational therapeutic strategies. An enhanced understanding of lncRNA biology could open more avenues to early AD diagnosis and treatment.
211 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 60122
Initial characterization of human skin stem cells
Neda Taheri, Faribe Esmaeili Department of Plant and Animal Biology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.
Objectives & Introduction: Since, there are different types of stem cells (SCs) in skin and its appendages; this tissue has considerable regenerative potential. Among all types of skin SCs, epidermal stem cells (EPSCs) are of particular interest, because they are numerous and easily accessible. In addition, there are no potential ethical and political limitations for EPSCs compared to embryonic stem cells. Similar to adipose-derived stem cells, EPSCs have been widely used in basic clinical studies and regenerative medicine. Materials & Methods: In this study, human skin stem cells isolated, cultured and expanded to passage three. Immunofluorescent staining carried out using anti β-integrin monoclonal antibody and FITC-conjugated secondary antibody. To evaluate the differential potential of the isolated EPSCs, osteogenic and adipogenic induction media were utilized and alizarin red and oil red staining were used to stain the treated cells. Furthermore, neural differentiation potential of the cells was examined using retinoic acid (RA) at the concentration of 5×10-7 mM. RA-treated cells evaluated by cresyl violet specific staining. Results: Initially, the expression of β-integrin in the isolated human skin stem cells proved by immunostaining. Osteogenic and adipogenic differentiation potential of the cells confirmed by alizarin red and oil red staining, respectively. In addition, immunofluorescence analysis showed the expression of nestin (a neuroepithelial marker) and synaptophysin (a marker for mature functional neurons) by RA-treated cells. The nuclei were counterstained by DAPI and primary antibody was removed as negative control for immunostaining. Conclusion: In the present study, the epidermal stem cells from human skin were successfully isolated, cultured and expanded to passage three. Furthermore, the cells were able to show osteogenic, adipogenic and neurogenic differentiation potential as confirmed by various methods. Keywords: Human stem cells; Epidermal stem cells; Stem cell chracterization; Beta integrin; Retinoic acid
212 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 61788
TEAD regulation and its impact on Cancer inhibition.
Fatemeh Shirazi, Zohreh Hojati
Department of Cell and Molecular Biology& Microbiology, Faculty of Biological Science and technology, University of Isfahan, Isfahan, Iran.
Abstract: In recent years, many studies have demonstrated that the dysregulation of Hippo pathway effectors is implicated in a wide range of cancers, including colon, prostate, lung, pancreatic, gastric, brain and liver cancer. The major transcription factors of this pathway are TEADs (TEA domain family). Many tumor types overexpress TEAD genes (TEAD 1- 4) and this modulation is directly correlated to the expression of pro-growth factors, such as CTGF, Cyr61, receptor tyrosine kinase AXL, Myc and survivin. It has also been shown that expression of TEAD has a direct correlation with mesothelin expression which is an important tumor marker. In gastric cancer, colorectal cancer and prostate cancer, inflated TEAD levels serve as a practical prognostic marker as TEAD expression levels correlate with poor clinical outcomes. Interestingly, in some cancers, TEAD expression can be downregulated, including renal and various breast cancers. TEAD has also an important role in cell migration in cancers like glioblastoma. Because of TEAD expression importance and the genes regulated by TEAD activity, TEAD proteins have become crucial mediators of tumorigenesis and malignancies and thus appealing therapeutic targets. Being able to simultaneously repress a variety of target genes by binding to their mRNA 3′untranslated regions (3′UTRs), microRNAs (miRNAs) have been found to play important roles in repressing tumorigenesis and malignant progression of human cancers. By these evidences targeting TEAD genes in cancers with specific microRNAs can be a confidential therapeutic strategy.
213 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 62671
The Effects of Mesenchymal Stem Cells on Tumors and Cancers
Mehrdad Ostadpoor 1*, Nasser Yazdani 1, Majid Gholami-Ahangaran 2, Seyyed Hossein Heidari 1, Pooria Rezaei 1
1. Doctor of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran 2. Department of Poultry Diseases, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran Introduction: Cancer is One of the Most Important Causes of Mortality in the Developed World. In General, an Uncontrolled Growth of Cells and the Absence of Apoptosis or the Gradual Death of Cells Cause Cancer. The Use of Stem Cells in Immuno-Modulation or Reconstitution is One of the Methods that has been Used for Decades in Cancer Therapy. Stem Cells have Self-Renewal Capacity with Highly Replicative Potential in Multilineage Differentiation Capacity and can be Divided into Three Main Categories, Including Embryonic, Germinal, and Somatic. But in this Way We have Different Problems Because Stem Cell have a Different Reactions. Description: In the Current Literature Review Keywords Including Cancer, Tumors, Apoptosis, Angiogenesis and Mesenchymal Stem Cell from the List of MeSH and other Credible Scientific Websites such as Science Direct, PubMed and Google Scholar were used to Compile the Effects of Stem Cell on Cancers and Tumors. Discussion and conclusion: Studies Show that MSCs has a Different Impact on Tumors and Cancer. MSCs can have an Impact on the Tumor Sites, Resulting in the Promotion of Tumor Growth and Angiogenesis through Differentiating into Cancer-Associated Myofibroblasts and Secretion of Proangiogenic and Enhanced Tumor Metastasis by Increasing Lysyl Oxidase. In addition, MSCs can Inhibit Natural killer Cell and Cytotoxic T Lymphocyte Functions in Breast Cancer. Studies have Demonstrated that Human Umbilical Cord MSCs can Promote the Growth of Lung Adenocarcinoma Cancer Cells by Transferring miRNAs. In Contrast, MSCs have Antitumor Effects in Different Animal Models of Cancers. MSCs can Suppress the Proliferation of Glioma, Melanoma, Hepatoma, and Breast Cancer Cells. It also has Some Antiglioma Effects through Inhibiting Vascular Growth in Glioma Cells. Moreover, Human Bone Marrow-Derived MSCs have the Potential to Suppress the Growth of Breast Cancer and Inhibit Lung Metastasis by Reducing their Proliferative Ability. Keywords: Cancer, Tumor, Apoptosis, Angiogenesis and Mesenchymal Stem Cell
214 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 63237
Unravel common comprising miRNAs in various types of cancers using comprehensive data-mining.
Samira Rahimirad1 1. Medical Genetic Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Introduction & Objectives: MicroRNAs (miRNA) are small non-coding RNAs that act as gene expression regulators in cells. Their crucial roles in the promotion of cancer have been established. MiRNAs could serve as potential biomarkers to accurately prognostic or providing a more efficient route for treatment. Materials & Methods: Literature mining was performed using the Keywords “miRNAs” and “Cancer” among articles deposited in Pubmed. The differential expression of various miRNAs in different cancers was collected. Then the common miRNAs among cancers were identified. MiRNA-Target gene regulatory network was constructed using Cytoscape. CytoHubba plugin was used to find hub miRNAs. Functional annotation analysis was performed using the DAVID tool. Results: Data-mining among 28 different kinds of cancer lead to collect 18 miRNAs that were deregulated in different cancer cells. Target genes of collected miRNAs were identified through constructing the regulatory network and miRNAs that had the most targets considered as hub including hsa-miR-17-5p, hsa-miR-320a, and hsa-miR-375 which had 319, 151, and 133 target genes respectively. Functional enrichment analysis of obtained miRNAs proved their role in cancer-related processes including apoptotic process, angiogenesis, and cell cycle. Based on evidence along with our obtained results, hsa-miR-320a, and hsa-miR-375 could consider as tumor-suppressor and hsa-miR-17-5p as oncomiR. Furthermore, the target discovery results revealed that these miRNAs play crucial roles through targeting genes involved in pathways such as Pancreatic cancer (map05212), Colorectal cancer (map05210), and Non-small cell lung cancer (map05223). Conclusion: Obtained results of this comprehensive data-mining lead to identifying the most important miRNAs that are common among various cancer and act as key elements in cancer progression. Functional analysis demonstrated that hsa-miR-17-5p, hsa-miR- 320a, and hsa-miR-375 are the most important miRNAs that could serve as accurate prognostic biomarkers for various cancers. KEYWORD: Cancer, microRNA, Biomarker, Data-mining.
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 63257
Coenzyme Q10 and its therapeutic potencies against COVID-19 and other similar infections: A molecular review
Mohammad Fakhrolmobasheri1, Mahnaz-Sadat Hosseini2, Seyedeh-Ghazal Shahrokh1, Zahra Mohammadi3, Mohammad-Javad Kahlani4, Seyed-Erfan Majidi1, Mehrdad Zeinalian1,3
1. Department of Genetics and Molecular biology, School of Medicine, Isfahan University of Medical sciences, Isfahan, Iran 2. School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences 3. Ala Cancer Control and Prevention Center, Isfahan, Iran 4. Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Sciences and Technologies, University of Isfahan, Isfahan, Iran
Abstract: New lethal Coronavirus infectious disease (COVID19), currently, has been converted to a disastrous pandemic worldwide. There is, now, no definite treatment for the disease and tens thousands of people have been died due it. The disturbance of renin-angiotensin system (RAS), a huge cytokine storm, altered metabolic pathways, severe mitochondrial dysfunction and huge oxidative stress are essential causes in molecular pathology of COVID19. Coenzyme-Q10 (CoQ10) is an essential cofactor in the electron transport chain of the phosphorylative oxidation system. CoQ10 is a powerful lipophilic antioxidant wih anti-apoptotic, immunomodulatory and anti- inflammatory properties which has been tested for the management and prevention of a variety of diseases specially diseases with inflammatory pathogenesis. CoQ10 as a free radical scavenger and a membrane stabilizer, prevents phospholipid peroxidation, and regenerates vitamin E (α- tocopherol) and vitamin C (ascorbate). although the effect of CoQ10 on gene expression still is not well understood but studies suggest that several genes specially genes contributing in inflammatory response, cell proliferation and mitochondrial function. The cardio-protective role of CoQ10 in improving viral myocarditis and drug induced cardiotoxicity has been determined in different studies. CoQ10 could also improve the interference in the RAS system caused by COVID19 infection through exerting anti-Angiotensin II effects and decreasing oxidative stress. CoQ10 raises the cellular metabolism and the patient’s response to oxygenation through improving the mitochondrial dysfunction via stabilizing the plasma membrane, sustaining the function of the + + NA /K ATPase, and regulating the oxidative phosphorylation system. CoQ10 passes easily through Brain Blood Barrier (BBB) and as a neuroprotective agent can reduce oxidative stress and modulate the immunologic reactions, which could decrease systemic inflammation, prevent BBB damage and neuronal apoptosis in COVID19 patients. Accordingly, CoQ10 supplementation could prevent the COVID19-induced morbidities and has a potential protective role against the deleterious consequences of the disease specially in patients under mechanical ventilation.
216 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 63473
Evaluation of immunomodulatory effect of Dracocephalum kotschyi extract on immune system in animal model
Hassan Sadraei1, Abbas Jafarian1, Gholamreza Asghari2, Mohamed Poladsanj1.
1. Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran. 2. Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
Introduction: Dracocephalum kotschyi is a native medicinal plant in Iran. D. kotschyi extract has been shown to inhibit lectin-induced cellular immune response in vitro. The objective of this research was to examine immunomodulatory action of D. kotschyi extract in animal model. Methods: To investigate the effect of D. kotschyi hydroalcoholic extract on innate immune system, mice left hind footpad thickness were measured and the D. kotschyi extract were administered via i.p. injection to the animals. An hour later, the animals were received an injection of sheep red blood cell (SRBC) into the left footpad (s.c). Footpad thickness was measured at 1, 2, 4, 6, 8, 24 and 48 hours intervals following antigen challenge. For investigation of effect of test drugs on acquired immunity, SRBC was injected (s.c) on the back of the animals and left in the cage for five successive days. On sixth day, SRBC was injecting into the left hind footpad and Footpad thickness was measured as above. Three groups of mice receiving hydroalcoholic extract (5, 10, 20 or 40 mg/kg). Betamethasone (4mg/kg, i.p.) and levamisol (4mg/kg, i.p) were used as positive control groups while normal saline was as negative control group. Data expressed as mean ± SEM (n=6) for each group of results. For statistical analysis one way analysis of variance (ANOVA) was used. This project was confirmed by the ethical committee of the university. Results: The results obtained indicated that levamizole potentiated the immune response while betamethasone inhibited both innate and adaptive immune responses. Hydroalcoholic extract of D. kotschyi markedly inhibited both innate and adaptive responses. The highest inhibitory effect was seen with extract dose of 20mg/kg (i.p.). Conclusion: This study confirms immunosuppressant effect of hydroalcoholic extract of D. kotschyi in inflammatory condition.
Keywords: Dracocephalum kotschyi, acquired immunity, innate immunity.
217 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 64298
Genomic instruments help to find Driver nodes in co-expression networks
Zoha Kamali1,2,3, Ahmad Vaez1,3* 1. Department of Bioinformatics, School of Advanced Medical Technologies, Isfahan University of Medical Sciences, Isfahan, Iran 2. Student Research Committee, School of Advanced Medical Technologies, Isfahan University of Medical Sciences, Isfahan, Iran 3. Department of Epidemiology, University of Groningen, University Medical Centre Groningen, Groningen, the Netherlands
Introduction & Objectives: One of the most important challenges in systems biology approaches has been finding driver nodes in network systems. The driver nodes are those who start the cascade of subsequent tiny changes to eventually reshape the whole system towards a new manner. These nodes can be used to engineer the system to yield significant and predictable impact and therefore are the best targets for drug design (1,2). Among different biological networks, co-expression networks have a special place as expression is almost always prior to other cellular changes. There are large-scale publicly available data sets from different tissues which are frequently used in network construction and analysis; however, focusing on the driver nodes is less studied in this field. Materials & Methods: Here we employed a Mendelian randomization (MR) approach to find genes whose expression changes the expression levels of a group of downstream genes. We applied the analysis on the largest expression data set generated to date (n ⁓32000) (3). We used the most significant single nucleotide polymorphism (SNP) associated with the expression level of each gene as instrumental variable (IV) and filtered out results with significant evidence of linkage confounding based on the heterogeneity test (HEIDI) in cis region of the gene (4). We tested each gene expression against the expression level of the nearby genes in cis region (1 Mb from each direction). We set the significance thresholds of both MR and HEIDI tests to Bonferroni corrected levels. Results: Our analysis returned 11162 expression quantitative trait genes (eQTGs) which cause changes in expression level of at least one downstream gene (p-value<2.61E-07 and HEIDI≥6.55E-07). Five eQTG hotspots, each affecting the expression level of more than 20 adjacent genes, are: E2F4, PRKAR2A, SLC12A4, RP11-96D1.5 and TRBV23-1. Driving nodes mainly consist of protein coding genes e.g. transcription factors (74%), regulatory RNAs e.g. antisense and lincRNAs (15%) and pseudogenes (7%). Conclusion: Our results help to find driver nodes in biological networks and hence to predict the resultant cellular and systemic phenotypes and also drug designs. As a next step,
our study can be expanded to also include trans co-expression associations to find even more and robust eQTGs.
218 Keywords: Driver nodes, network, systems biology, MR, co-expression
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 65011
Determination of Antioxidant Potential, Phenolic Content, Flavonoid Content and Antitumor Effect of Trachyspermum ammi seed Essential Oil on Breast Cancer Cell lines MDA-MB-231, MDA-MB-468, Mcf7
Alireza Molazadeh1, Amin Dakhili Ardestani2 1. Fasa Medical plants Research center 2. Student Research Committee of Fasa University of Medical Sciences Background: Cancer is one of the leading causes of mortality worldwide and breast cancer is the second most common cancer among people. The persistent presence of saturated free radicals in the natural cells of the mutant causes the cancer to weaken by weakening the antioxidant system. Plants contain antioxidants that reduce the incidence and progression of cancers by reviving free radicals. The aim of this study was to determine the phenolic content, flavonoid content, antioxidant power and antitumor effect of essential oil of Trachyspermum ammi seed. Materials and Methods: In this in vitro experimental study, phenolic content of essential oil of Trachyspermum ammi seed using Folin's reagent, flavonoid content of this extract using Aluminum Chloride (AlCl3), essential oil antioxidant activity The use of two methods (DPPH) of 2,2-diphenyl-1-picrylhydrazyl and Ferric reducing ability of plasma (FRAP) at different concentrations were evaluated, and the antitumor effects of this essential oil by MTT assay on MDA- cell lines. MDA-MB 231, MDA MB-468, Mcf7 were evaluated. Results: Phenolic content of the essential oil of Trachyspermum ammi seed 429/63±25/52 µgr GAE/mgr dw, Flavonoid content of this essential oil 467/17±10/75 µgr EQ/mgr dw, monofluorescence essential oil Adult seed was obtained 374/40±41/68 μMFe2+/gr And for inhibition of DPPH free radicals, IC50 of 4934 μg/ml was obtained.The mean IC50 was obtained for Mcf7 cell line 222.3,MDA-MB-468 334/03,MDA-MB-231 291/03µg/ml. Conclusion: Trachyspermum ammi seed with remarkable antioxidant power at high concentrations are likely to be able to inhibit free radicals and can maintain cancer balance by maintaining antioxidant system and cell homeostasis. Prevention. It is recommended that in future animal studies or clinical trials, the therapeutic effect of this plant on the reduction of tumor growth and proliferation of cancer cells be investigated. Keywords: Extract, Antioxidant, Phenol, Flavonoid, DPPH, FRAP, Breast Cancer, MTT
219
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 65661
Bioinformatics study of single nucleotide polymorphism rs3733890 and hsa- miR-185-5p related to it in Acute Lymphoblastic Leukemia
Samereh Gholizadeh1*, Massoud Houshmand2
1. Department of Genetics, Department of Biology, High Institute nurdanesh, Meymeh, Isfahan, Iran. 2. Medical Genetic Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Introduction & Objectives: Acute lymphoblastic leukemia accounts for 80% of childhood leukemia. BHMT is one of the important genes in this cancer. One of its important functions is the production of methionine. Rs3733890 is located on the BHMT gene and contains the G allele (dominant allele) and the A allele (recessive allele or SNP). The aim of this study was to evaluate the role of BHMT gene and its association with has-miR-185-5p in the pathway of acute lymphoblastic leukemia. Materials & Methods: At first, the TARGETSCAN database was used to identify BHMT- related microRNAs. After investigating the binding site of related microRNAs, hsa-miR- 185-5p was selected. This microRNA binds to the region that rs3733890 is located. The target genes hsa-miR-185-5p were obtained from the miRWALK2.0 database. The associated signal pathways were identified by using the DAVID and KEGG databases. Results: Based on the information obtained from the articles, the risk of SNP allele for this polymorphism was considered as a research hypothesis. Although according to the articles, hsa-miR-185-5p is a tumor suppressor that has been downregulated in cancer. Due to downregulated expression, microRNA less binds to the gene and has less inhibitory effect. In this case, gene expression is increased. Conclusion : When the SNP allele is present, BHMT have been overexpression due to less inhibition by hsa-mir-185-5p and leads to cancer pathway. As a result, this gene is an oncogene in the cancerous pathway. Increased gene expression leads to increased methionine production and feeding of tumor cells to methionine and promote cancer. Based on the research hypothesis, it is expected that by designing primer at rs3733890, the SNP allele will be in leukemia patients with in-vitro test. It is predicted that the presence of the SNP allele in the BHMT gene may increase the risk of cancer and can be used as a prognostic factor for acute lymphoblastic leukemia. Keywords: leukemia, BHMT, hsa-mir-185-5p, rs3733890
220 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 66112
Biological concepts and potential clinical implications of piRNA in cancer
Zahra Ghaseminezhada, Mohammadreza Sharifia* aDepartment of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Introduction: PIWI-interacting RNAs (piRNAs) are small non-coding RNAs with 24-35 nucleotides in length that were first identified in Drosophila testis. The similarity of piRNAs to other small non-coding RNAs, such as miRNA and siRNA, is that they all interact with members of a similar family of Argonaute proteins and have a regulatory role in gene expression, But the biogenesis pathway of piRNAs differs from the other two small non-coding RNAs. In the human genome, there are more than 20000 piRNAs that play important role in regulating gene expression. Many studies have shown the aberrant expression of piRNAs as a unique feature in different types of cancer. Description: Cancer is the second leading cause of death in the world that treatment available for it are often ineffective and have high rates of recurrence and metastasis, so we need new biomarkers to diagnose and treat cancer. The RNA sequencing technique shows that piRNA have aberrant expression in different cancers, so they can be used as biomarkers to diagnose different types of cancer, including breast cancer, gastric cancer, lung cancer and more, in which the article is reviewed. Discussion and conclusion: In recent years, with the development of next-generation sequencing technology, we have been able to compare the expression level of different small non-coding RNAs, including piRNAs, between normal and cancerous cells. Recently, many piRNAs have been shown to be highly expressed in blood samples, so they can be used as biomarkers for cancer detection without the need for invasive procedures. However, further research is needed to understand the molecular mechanism of piRNAs and its clinical applications.
221 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 66803
The role of lncRNAs in Hepatocellular carcinoma
Zahra Pourghorban Saghati1,*, Asiyeh Jebelli2
1. Student of Cellular and Molecular Biology, Department of Biological Science, Faculty of Basic Science, Higher Education Institute of Rab-Rashid, Tabriz, Iran 2. Assistant professor, Department of Biological Science, Faculty of Basic Science, Higher Education Institute of Rab-Rashid, Tabriz, Iran Introduction: Hepatocellular carcinoma (HCC) is one of the most common and aggressive human malignancies. The dismal clinical outcome of HCC is largely due to the high incidence of intrahepatic and extrahepatic metastasis in HCC patients. Long non-coding RNAs (lncRNAs) are a group of non-protein-coding RNAs that are greater than 200 nucleotides in length. They serve as either oncogenes or tumor suppressor genes and play a vital role in tumorigenesis. Description: Several different lncRNAs, including HULC, HOTAIR, MALAT1 and H19 were identified that are deregulated during HCC tumorigenesis and metastasis. HULC with 1.6 k nucleotide long contains two exons but not translated. Its upregulation deregulates lipid metabolism and promotes metastasis in HCC. Furthermore, HULC inhibits apoptosis mediated by miR-9. HOTAIR has been reported to be an oncogene that influences tumor cell development and correlates with prognosis in HCC by repressing RBM38. MALAT1 promotes the migration and invasion of HCC by mediating of miR-204 and miR-200a pathways. MALAT1 knockdown inhibits the proliferation, migration, and invasion, and induces apoptosis in hypoxia-challenged Hepatocells. These results indicate that MALAT1 plays an important role in tumor progression and could be a novel biomarker for predicting tumor prognosis. The H19 gene encodes a 2.3 k nucleotide lncRNA that is exclusively expressed from the maternal allele. H19 downregulates in HCC is correlated with its decreased promotor methylation. Discussion and conclusion: Abnormal expression of lncRNA might interrupt gene expression in genetic and epigenetic level and was associated with prognosis of hepatocellular. LncRNAs that are found in body fluids could detected by next generation technologies. This implied the possibility of lncRNA to become a diagnosis biomarker and therapeutic target of HCC.
222 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 67207
Preplasmic expression of a tandem scvf antibody for simultaneous targeting of two immune check-points
Rashti AR, AKbari V Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran.
Introduction: Immune checkpoints are regulators contributing in self-tolerance and helping immune homeostasis. However, some cancer cells activate them to prevent anti- tumor immune response. Immune check-points inhibitors have been proposed to help immune system identify andkill tumor cells with promising results in clinic practice. . Here, we aimed to produce a novel bispecific antibody (tandem scvf) originated from nivolumab and ipillimumab for simultaneous targeting CTLA-4 and PD-1 check-points. Methods: Thebispecific tandem scvf antibody gene was designed based on amino acid sequences of variable regions of parent antibodies and codon optimized for expression in bacterial system. Synthetic gene was ligated into suitable expression vector (pET22b) before pripaslmic signal peptide. E. coli BL21 (DE3) was used as a host cell for protein expression.Protein expression at different temperatures (23, 30, 37 ºC) and with different inducer concentrations (0.1, 0.5, 1 mM) was done. After periplasmic extraction, antibody was purified by affinity-chromatography. Results: Expression of tandem scvf antibody (60 kDa) was confirmed by SDS-page and western blot. The optimum expression condition was found to be induction with 0,5 mM IPTG and incubation at 30 ºC. Biological assay of purified antibody is is going to be performed by the research group. Conclusion: Optimization of expression condition could improve periplasmic expression of scfv antibody. This scvf could be a potential candidate to targeting immune check- points, although in vitro and in vivo biological function evaluations still need to be performed.
223 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 68513
Design and evaluation of two novel chimeric proteins, p28-IL-24 and p28-M4, targeted to breast cancer cells: An In-Silico approach
Reza Ghavimi 1,2, Elmira Mohammadi 1,2, Vajihe Akbari 1, Fatemeh Shafiee 1, Ali Jahanian-Najafabadi 1* 1. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran 2. Student Research Committee, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
Introduction & objectives: p28 is an anticancer peptide (ACP) shown to be cytotoxic against various cancer cells. Moreover, as a cell penetrating peptide (CPP), p28 can be applied as a targeting moiety in the structure of chimeric proteins. IL-24 (or its truncated sequence, M4) is a cytokine with anticancer activity against a wide range of malignant cells. We aimed at production of chimeric proteins composed of P28 and either IL-24 or M4 to target breast cancer cells. However, selection of a suitable linker to join the two moieties is an important factor in the design of chimeric proteins. In the current study, the effect of different linkers on construction of the two chimeric proteins (p28-IL-24 and p28- M4) were evaluated in silico. Materials & Methods: After selection of some linkers with different characteristics, a small library of the chimeric proteins were created and assessed by various online servers and bioinformatics software. Furthermore, following selection of the most appropriate linker, the three dimensional structures and dynamic behavior of both chimeric proteins were evaluated by homology modeling and molecular dynamic simulation (MD), respectively. Results: Based on our results, a rigid linker with AEAAAKEAAAKA sequence showed to provide the highest freedom of action for the both of proteins. Furthermore, according to MD parameters, between p28-IL-24 and p28-M4 chimeric proteins, the first one have better solubility as well as stability and might show stronger anticancer effects in vitro and in vivo, because its moieties showed to exert their activities more freely. Conclusion: Taken together our fining showed that p28-AEAAAKEAAAKA-IL-24 has better stability and solubility than the p28-M4 protein. Therefore this chimeric protein was anticipated to show better anti-cancer efficacy. However in vitro and in vivo studies are needed to assess their biological activity and cytotoxic effects. These studies are undergoing for the two chimeric proteins. Keywords: p28; IL-24; M4; Linker; Chimeric protein; Homology modeling; Molecular Dynamic Simulation, Breast cancer
224
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 68807
Modern dressings in the treatment of diabetic ulcer
Cheshmeh Habibi¹, Farzane Jabari², Reza Salarian¹
1. Department of Biomedical engineering (Biomaterial), Maziar HigherEducation Institute, Royan Iran 2. PhD Biomedical engineering (Biomaterial), Nanotechnology andAdvance Material Department, Materials and Energy Research Center (MERC), Alborz Iran 3. Department of Biomedical Engineering (Biomaterial), Maziar HigherEducation Institute, Royan Iran
Background and objective: Dressing is the biocompatible material covering the wound and injury caused by trauma, incision, contusion and burn. This type of dressing in the process of direct contact with the body tissue and in a reciprocal strain can improve wound healing and litter environment more quickly than standard or traditional dressing. In the past, in traditional dressing, it was believed that, keeping the wound dry would help regenerate it, but in recent years, the principles of dressing have changed, and today, the effort to maintain the injuries due to the rate of discharge and the exudate gets wet on a periphery. Modern dressings make the best of this. Search method: In the context of this overview, 13 articles were used between the years 2010 through the end of 2018 using the Keywords of PubMed, Google scholar search engines. Results: Modern dressings are proprietary, which means that different materials are used depending on the type of wound. Modern dressings make faster healing, have stronger germicidal properties and maintain moisture in the wound area. Also, they do not cause scarring in the wound area. This will accelerate wound healing. Conclusion: Modern dressing is a creative idea for treating chronic and infectious wounds and types of wounds that are large or complex. Unlike traditional dressing, which is used dry and alone and requires a knot or adhesive wound, the new dressing is usually self- adhesive. With the advancement of medical science, it has been found that controlled humidity not only does not worsen the wound, but is also very useful in the treatment of ulcers preventing chronic scarring. Today, dressing from a mere protective coating has become a treatment for a variety of wounds and scars. The special design and structure of the advanced dressings and compounds used in them, have provided many therapeutic benefits. Today, dressing is not just a bandage, it is produced in various forms, such as powder or gel for special purposes. Keywords: Wound dressing, Modern dressing, Treatme
225 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 69257
Silybum Marianum for Wound Healing
Elnaz Najafi¹*, farzaneh jabbari², Reza Salarian3
1. Department of Biomedical Engineering, Maziar Higher Education Institute. Royan, Iran 2. Ph.D of biomedical engineering,Nanotechnology and Advanced Materials Department, Materials and Energy Research Center (MERC), Alborz, Iran 3. Department of Medical Information, school of Medicine, isfahan University of Medical Sciences, Isfahan, Iran Introduction: Silybum marianum has an uncurled, pale green plant, with red to purple flowers. There are a lot of compounds in this plant, including such as saline, silymarin, apigenin, and et. Silymarin is a complex mixture of polyphenolic molecules, including seven closely related flavonolignans and one flavonoid. Poor water solubility and bioavailability of silymarin led to the development of enhanced formulations. Description: In the context of this overview, 12 articles were used between the years 2010 through the end of 2018 using the Keywords of PubMed, Google and Google scholar search engines. Discussion and conclusion: One of the goals of medical science is wound healing in shorter time and with less side effects. The results indicate that the extracts of Silybum Marianum accelerate the repair and restoring process of the wound;Silibinin affects collagen synthesis. Silymarin improves epithelization and it has no effect on the percentage of wound contraction. Several studies demonstrated that Silybum Marianum due to anti- inflammatory substances, has an important effect on the first phase of wound healing.However, the study of the effects of its active compounds on wound healing and tissue engineering can be important. Keywords: Skin, Wound healing, Silibinin, Silymarin
226 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 70053
Host-Directed Drug Therapies for leishmaniasis
Simindokht Soleimanifard Department of parasitology and mycology, school of medicine, Isfahan University of medical science
Introduction: Host-targeted drugs bypass many of the problems encountered by treatments that specifically target parasites, by acting directly on host molecules or pathways that are redundant for the host but critical for a pathogen. Such approaches are likely to have less chance of developing resistance as the host molecules and processes mutate at lower rates than most pathogens. Additionally, because these drugs act on the host, these treatments may be broad-spectrum and effective against several pathogens. Description: Different strategies have been employed to identify new host targets. The more general approaches are transcriptomic and proteomic analysis as well as the assessment of microRNA, small interfering RNA (siRNA) and short hairpin RNA expression profiles. Functional genomics has also been used to study gain or loss of function by over-expressing cDNA or iRNA respectively in mammalian cells to investigate the effects of different phenotypes on the pathogenesis of intracellular pathogens. Additionally, hybrid interaction screens can be used to study protein-protein interaction between the host and the pathogen and can help identify potential host targets for drug therapy. Another method used to identify protein-RNA interactions is affinity chromatography. Discussion and conclusion: Despite the high prevalence of leishmaniasis, a tropical parasitic infection, only a handful of treatments available against this parasite (Sbv, miltefosine and amphotericin B). Many of those exhibit high toxicity and increasing parasitic resistance. Recent studies have identified several host-targeted therapeutics. These approaches include the use of immuno-modulators, kinase inhibitors, and also natural compounds, which activate pro-inflammatory transcription factors like NF-Kb. In these treatments, immunomodulators are promising therapeutics not only used by themselves but also in combination with other drugs. Based on what has been said, some discovered drugs are: Keywords: Imatinib, Phosphoinositide 3-kinase γ (PI3Kγ),AS101 (ammonium trichloro [1,2-ethanediolato-O,O’]-tellurate), Ibrutinib, Berberine chloride, Statins , Naloxonazine, Pentalinonsterol , Oleuropeinis, Mahanine, Fucoidan, Artemisinin, Eugenol, Propolis.
227 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 70850
Bioinformatics evaluation of has-miR-514a-3p associated with single nucleotide polymorphism (rs143847362) TET2 gene and myeloid cancer.
Niloofar Ataee1.*, Niusha Ataee2.*, Massoud Houshmand3.*, Nasrin Fatahi4, Atefeh Zamani5
1. Islamic Azad University of Falavarjan , Isfahan ,Iran ([email protected]) 2. Biotechnology Division, Department of Biology, Shahi Ashrafi Esfahani Nonprofit University, Isfahan, Iran ([email protected]) 3. Medical Genetic Department, National Institute for Genetic Engineering and Biotechnology ([email protected]) 4. Gene RAZ BUALI, Genetic and Biotechnology Academy, Isfahan, Iran ([email protected]) 5. Gene RAZ BUALI, Genetic and Biotechnology Academy, Isfahan, Iran ([email protected])
Abstract: Myeloid acute leukemia is one of the types of leukemia this type of leukemia affects bone marrow or myelocyte cells and there is an acute process. With using different bioinformatics, we selected a tumor suppressor geneTET2, that is eliminated or mutated in patients with various myeloid cancer. TET2 expression of hematopoietic cells is the highest in granulocytes. healthy tissues, the expression of hematopoietic cells is the highest in granulocytes. Increased in accordance with the function in myelopoiesis. For this reason, NCBI, MiRWALK0.2, miRBase, miRNASNP, DAVID sites were used to find more bioinformatics information. Interaction between the target and the microRNA based on the 3`UTR binding region of the extracted microRNA site and in order to find the most relevant signal paths, DAVID was compiled and the paths associated with it were evaluated on the KEGG database. The Gibbs free energy for the interdiction between the has-miR-514a-3p and TET2 with allel A inregion of rs143847362 is -24.20 and Gibbs free energy for the interdiction between the has-miR-514a-3p and TET2 with allel G inregion of rs143847362 is -23.90 .Therefore, it is expected that at the specific site of rs143847362 in the presence of the G allele, hsa-miR-514-3p have less binding and inhibitory effect. Therefore, gene expression in these individuals with the G allele is predicted to increase and we expect the G allele to be more common in sick people. Therefore, given the oncogenic role of the gene and the KEGG pathway, it is expected to be less common frequent in patients with the A allele. Keywords: Myeloid cancer, TET2, rs143847362, Inhibitory effect.
228 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 71281
Expression changes of miR-29c-3p in glucose-affected mesenchymal stem cells
Mohammad Reza Mehrabi (PhD)*1, Reza Yari (PhD)2, Somayeh Mansournejad (MSc)2 1. *Department of Laboratory Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran. 2. Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Iran.
Introduction & Objectives: Mesenchymal stem cells are the essential support of the body tissues and are the appropriate choice for cell therapy for various diseases, including diabetes. miR-29c-3p is a type of microRNA that controls a network of effective genes in the tissue repair process and hyperglycemia in diabetics and seems to affect the expression of miR-29c-3p in mesenchymal stem cells and the ability of these cells to repair the tissue- related complications of diabetes. Evaluation of the effect of various concentrations of glucose on the miR-29c-3p expression in mesenchymal stem cells was aimed at this study. Materials & Methods: Mesenchymal stem cells were cultured in DMEM medium containing three different concentrations of glucose 250 mg/dl(chronic diabetes), 140 mg/dl(mild diabetes) and 100 mg/dl(normal). After 72 hours, RNA was extracted from these cells and miR-29c-3p expression was performed by Real-Time PCR technique and the results were analyzed using REST software. Results: The expression of miR-29c-3p in cultured cells in mild and chronic diabetic conditions was significantly reduced compared to normal conditions (P˂0.05). Conclusion: Considering the role of the increased expression of miR-29c-3p in various cellular processes affecting tissue repair including reduction of proliferation, differentiation, cell migration, extracellular matrix synthesis, angiogenesis and increased apoptosis, the reduction of miR-29c-3p expression in mild and chronic diabetic conditions in mesenchymal stem cells may reduce the ability of these cells to repair tissue damage caused by diabetic fibrosis and increases the ability of these cells to repair diabetic wounds. However, the explicit confirmation will require In vivo testing. Keywords: Diabetes, Mesenchymal Stem Cells, miR-29c-3p.
229 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 71562
The Effect of Decitabine on the Expression and Methylation of the PPP1CA in Association with Changes in miR-181b in NALM6 Cell Line
Asma Vafadar (1) (*), Niloufar Nazemoroaya (2), Gholamhossein Tamaddon (3) 1. Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran 2. Department of Basic Science, School of Medicine, Faculty of molecular Genetics, Science and Research Branch, Islamic Azad University, Tehran, Iran 3. Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract: Precursor B-cell acute lymphoblastic leukemia(B-ALL) is the most widespread pediatric malignancy and is a highly diverse disease characterized by cytogenetic and molecular abnormalities, including altered microRNA (miRNA) expression signatures. DNA methylation and modifications in the microRNAs expression are identified to be significant reasons for B-ALL. Decitabine as a DNA methyltransferase inhibitor agent is able to provoke hypomethylation in various tumor suppressor genes. Much proof has shown PPP1CA act as a tumor suppressor gene in numerous malignancies. In the current research, the mRNA expression of PPP1CA gene using qReal-time PCR in Nalm6 cell line and five patients suffer from ALL with mean age 5.6 years were determined in comparison with seven normal healthy donors age and sex-matched. Quantitative Real-time PCR investigation exhibited that the expression level of PPP1CA gene was significantly reduced in Nalm6 and according to the Methylation-specific PCR (MSP) analysis, this gene was hypermethylated in Nalm6. In the next step, the effects of decitabine treatment on the methylation and expression of this gene in association with changes in miR-181b expression levels were assessed in an optimal concentration of 2.5 µM of decitabine. Our data confirmed that decitabine is capable to restore the expression level of the mentioned gene and down-regulate expression level of oncomir 181b in the Nalm6 cell line. Consequently, it appears that decitabine can be utilized as a potential drug for the first-line treatment of B-ALL patients, but further in vivo study is required. Keywords: B-ALL, DNA methylation, MicroRNA, Decitabine, NALM6
230 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 71626
Losartan alleviates kidney fibrosis via downregulation of collagen IV in rats with unilateral ureteral obstruction
Sara Hosseinian1,2*, Abolfazl Khajavi Rad1,2
1. Assistant professor of physiology, Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran 2. Associate professor of physiology, Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Background and Objective: Unilateral ureteral obstruction (UUO) causes severe renal tubule-interstitial fibrosis. Renin-angiotensin system plays an important role in pathophysiology of UUO. Because of many in the present study, the effects of loeartan against kidney fibrosis were investigated in rats with UUO. Materials and methods: 30 male Wistar rats were divided into 3 groups: Sham operated, UUO, and the animals with UUO treated with losartan. At the 4th day of the experiment, the animals were anaesthetized and then, the abdomen was opened with a midline abdominal incision and the left ureter was ligated with 4-0 silk at two points. The administration of losartan was initiated since the first day and was continued for two weeks after UUO. At the last day of the experiment, the left kidneys were removed. The expression of Collagen IV and transforming growth factor (TGF)‐β1 were meausured by immunohistochemistry. Renal interstitial fibrosis was assessed by Masson's trichrome staining. Results: Renal expression of TGF‐β1 and collagen IV, as well as interstitial fibrosis significantly increased in UU rats. Losartan significantly downregulated the expression of these fibrotic markers and interstitial fibrosis. Conclusion: Losartan could be regarded as a therapeutic agent for treatment of UUO‐ induced kidney fibrosis. Keywords: Losartan, Unilateral ureteral obstruction, Fibrosis
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 71825
Cell-free DNA: Fast tool for diagnosis
Samira Nafar1 1. Department of medical genetics, Faculty of medicine, University of medical science, shiraz, Iran
Introduction: Diagnosis is the most important step in different diseases, especially in cancers. Recently, there are different methods in order to better diagnosis of diseases, but many of them are invasive and also, some of them are not useful for immediate diagnosis. Cell-free DNA (cfDNA) is one of the non-invasive prognostic biomarkers in different diseases such as solid tumors. In fact, amounts of cfDNA in serum or plasma can be used for diagnosis. Description: In this study review, we have used 100 articles published from 2015 to 2019 in the Google Scholar, PubMed, Web of Science and Update databases. we have considered some cancers such as hepatocellular carcinoma, lung cancer, breast cancer to compare the various methods of cfDNA diagnosis. Discussion and conclusion: In HCC patients, the level of serum cfDNA be used as biomarker. Increased amount of cfDNA in HCCs is associated with poor survival. Lung cancer is one of cancers which is diagnosed in advanced stage. Use of cfDNA can be as reliable and faster test than tissue-genotyping for eight guide line recommended biomarker (EGFR mutations, ALK fusions, ROS1 fusions, BRAF V600E mutation, RET fusions,
MET amplification and MET exon 14 skipping variants, and ERBB2 (HER2) mutations) . 38T There are several way to diagnosis of breast cancer contain: cancer antigen 15-3, gene alternations, changes in DNA methylation and cfDNA which has high concentration in blood of patients so has potential to be a good diagnosis tool. Among of them concentration
of cfDNA in blood of BC patients is very sensitive and informative. 38T There are different methods for diagnosis of these malignancies. Recently, measurement of circulating cell free DNA (cfDNA) in serum or plasma is used as a new diagnostic tool in different diseases such as cancers, sepsis, trauma, etc.
232 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 72145
Effect of flaxseed and chamomile extract on concentration of some biochemical serum factors in rats with experimental gastric ulcer
Seyed Mahdi Banan Khojasteh1, Saniya Akbary Dibavar2 1. Associated professor, Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran. 2. Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran.
Introduction and Objectives: Non-steroidal anti-inflammatory drugs (NSAIDS) are used in the treatment inflammatory diseases and these medications can sometimes have various side effects. It has been shown that some herbs can reduce or improve the side effects of these drugs. This study was performed to investigate the effects of chamomile and flaxseed extracts on biochemical factors (Glucose, Potassium, Urea, Calcium, Phosphorus, Sodium, Copper) after induction of indomethacin gastric ulcer in 5 groups of adult male rats. These groups were: negative control group (C), group with 50ml dose of the plant extract (5TE), group with 200ml dose (20TE), group with 500ml dose (50TE), positive control group (In). Materials and Methods: In this study, 30 male Wistar rats were used. The treated rats received extracts toward body weight by Gavage method for 14 days. At the end of treatment, gastric ulcer was induced by indomethacin at a dose of 30 mg for one day. Four hours after induction of gastric ulcer, rat’s blood was collected and biochemical factors of all groups were evaluated. Results: The results showed that Glucose, Copper and Potassium values of the 50TE group did not change significantly compared to the other groups. The amount of Urea in group 50TE is significantly reduced compared to group C. Calcium levels in the 5TE and 20TE groups did not change significantly, but in the 50TE group, there was a significant decrease compared to group C. Conclusion: The combination of Chamomile and flaxseed extract can have a protective effect against gastric ulcer and changes in serum biochemical factors with its antioxidant properties. Keywords: Chamomile, Flaxseed, experimental Gastric ulcer, Biochemical factors, Rat.
233 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 72202
Evaluation of single nucleotide mutation in exon 1 of myoc gene in patients with primary congenital glaucoma
Mansour Heidari (PhD)*1, Reza Yari (PhD)2, Ramin Nobari (MSc)2 *1Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran. 2Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Iran.
Introduction & Objectives: Vision is the most vital human feeling and glaucoma is one of the most important causes of blindness in the world and Iran. In glaucoma, progressive and persistent destruction of the optic nerve axons occurs due to non-genetic and genetic reasons, including a mutation in the myoc gene encoding the myocilin protein. The purpose of this study was to determine the presence of a point mutation in exon 1 of myoc gene. Materials & Methods: In this study, 30 families with PCG were sampled after obtaining the approval of a medical and genetic counselor, obtaining a consent, and observing a patients' rights convention. Also 100 control samples were used in the study. Arms-PCR was performed by Pfu enzyme with specific primer exon 1. Data were analyzed by Chromass and then Mega 6 programs at the significant level P˂0.05. Results: The results showed that G> A mutation was significant at position 227 of exon 1, which converts AAA to AGA code, so arginine replaces lysine. Conclusion: The presence of this gene, although not the cause of PCG alone, can be effective in improving clinical investigations and the effective gene network in PCG. By studying the changes in ocular pressure and the presence of mutations in different exons of this gene, the complete relationship between these two factors can be plotted. Keywords: Glaucoma, myoc gene, Arms-PCR, single nucleotide mutation.
234 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 72704
The role of LncRNAs in endometriosis
Gholamreza Rezaei Danbaran Student Research Committee, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Background: Endometriosis is a complex, heterogeneous, chronic inflammatory condition impacting 176 million women worldwide. However, the etiology of endometriosis is still unclear. Recently, some research has suggested that the occurrence and development of endometriosis may be related to genetics. Long non‐coding RNAs (lncRNAs), longer than 200 nucleotides in length, are mRNA‐like transcripts and cannot be translated into proteins. This class of non-coding RNA, have been shown to be essential in many diseases, like infertility. Scientists found that ectopic endometrial cells taken from women with endometriosis showed elevated levels of lncRNA-H19, with expression levels correlating to disease progression. Knockdown of H19 in ectopic endometrial cells inhibited cell proliferation and invasion. Coinciding with this change was an increase in microRNA-124-3p (miR-124-3p) and a decrease in integrin beta-3 (ITGB3) levels. The addition of a miR-124-3p inhibitor mitigated this decrease in ITGB3. Up-regulation of miR-124-3p markedly suppressed ITGB3 expression by binding to the 3' untranslated region (3' UTR), while inhibition of miR-124-3p had the opposite effect. ITGB3 overexpression potently counteracted the effects of miR-124-3p mimics on ectopic endometrial cells. From these results, scientists found that in endometriosis both miR-124- 3p and ITGB3 operate as downstream effector proteins in the H19-signaling pathway. Down-regulation of lncRNA-H19 could inhibit ectopic endometrial cell proliferation and invasion by modulating miR-124-3p and ITGB3. LncRNA MALAT1 showed upregulation in ectopic endometrial tissues. Transfection of MALAT1 siRNA suppressed its expression in endometrial cells, inhibited cell proliferation or invasion, enhanced caspase 3 activity, decreased NF-κB/iNOS or MMP-9 expression. also, LncRNA BANCR inhibitor can repress the development of ectopic endometrial tissues by inhibiting the generation of angiogenic factors in the EMs focus, and its mechanism may be related to the inhibition on the ERK/MAPK signaling pathway. In conclusion, endometriosis is a heterogeneous disease that can be diagnosed and treated by using of markers such as LncRNAs. Keywords: Endometriosis, LncRNA
235
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 73186
Bioinformatics study of single nucleotide polymorphism rs2279744 and hsa- miR-10a-3p related to it in Acute Lymphoblastic Leukemia
parvin sheybani*1, Massoud Houshmand2
1. Department of Genetics, Department of Genetics, Department of Biology, High Institute nurdanesh, Meymeh, Isfahan, Iran. 2- Medical Genetic Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran Introduction & Objectives: Leukemia is the fifth most common cancer in the world. Acute lymphoblastic leukemia is the most common type of leukemia in children. MDM2 is one of the important genes in this cancer. MDM2 plays an important role in P53 degradation. Increaseing levels of MDM2 protein, promotes tumor formation. Rs2279744 is located on the MDM2 gene and has T alleles (dominant allele) and G allele (recessive allele or SNP). The aim of this study was to evaluate the association of rs2279744 polymorphism and related hsa-miR-10a-3p, due to the role of MDM2 gene in leukemia cancer pathway. Materials & Methods: In the first step, TAEGETSCAN7.2 database and hsa-miR-10a-3p were used to identify microRNAs associated with MDM2 gene. This microRNA binds the region of rs2279744 single nucleotide polymorphism. By confirming the role of has-miR- 22-5p in leukemia via the phenomiR database, it was found that this microRNA is down- regulated in leukemia. The hsa-miR-10a-3p target genes were identified from the miRWALK2.0 database and the related signal pathways were analyzed by using the DAVID and KEGG databases. Results: According to the data obtained from paperes, the risk of SNP allele in cancer in the population was considered as a research hypothesis for this polymorphism. According to the pathway obtained from the KEGG database, being oncogeneic was confirmed for the MDM2. Due to the decreased expression of hsa-miR-10a-3p, less inhibited MDM2 will result in increased expression. Conclusion: In fact, in the presence of SNP allele, gene is overexpressed and overly degrades p53 (tumor suppressor protein). It can be predicted that the presence of the SNP allele in rs2279744 increases the possibilityof being prone to cancer. It is expected that by designing primer at rs2279744, the SNP allele will be in leukemia patients by in-vitro testing. On the other hand, this is a possibility that the presence of the SNP allele decrease the risk of cancer. Keywords: leukemia, MDM2, hsa-miR-10a-3p, rs2279744
236 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 74145
Study of cytotoxic of Glaucium Flavum Extract on HT-29 Cancer Cells
Sima Orouei1*, Ebrahim Rahmani Moghdam2, Shohre Alian Samakkhah3, Kiavash Hushmandi4
1. MSc. Student, Department of Genetic Science, Medical Science Branch, Islamic Azad University, Tehran, Iran. Email:[email protected] 2. Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. 3. Department of Food Hygiene, Faculty of Veteriary Medicine, Amol University of Special Modern Technologies, Amol, Iran. 4. Department of Food Hygiene and Quality Control, Division of Epidemiology & Zoonoses, Faculty of Veteriary Medicine, University of Tehran, Tehran, Iran.
Introduction and Objectives: Glaucium flavum is a plant from Papaveraceae family; this plant is rich in alkaloidal components including Aporphine, protoberberine, and Glaucine. Recent research has also added antioxidant, anti-cancer, and antiviral properties to potential uses of the plant. Therefore, this study aimed to evaluate the effect of this extract on the cellular cytotoxicity of HT-29 cell line. Material and Methods: In an experimental study, the inhibitory effect of the extract at concentrations of 25, 50, 100, and 200 (μg/ ml) on the viability of HT-29 cells was investigated. Giemsa staining technique was used to study cell morphology, and SPSS version 17 was used for statistical analysis. Results: MTT results showed dose and time dependency of this extract with a reduction in cell viability, and the concentration of 50 µg/ml was used for the determination of IC50. The morphology of the cells was characterized by cell flaccidity with damage to the cell membrane. Conclusion: This extract can lead to cell death in the HT-29 cell line as a result of the effects of its alkaloids. Keywords: Glaucium flavum, MTT-assay, HT-29.
237 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 74755
Insilico study of bioactive compounds having similar structure to cell death inducer natural products in Cancer
Marzieh Abolghasemi1*, Fatemeh Kheiri1, Negar Hosseini1 1. Genetic Division, Department of Biology, Faculty of Basic Science, Shahrekord Univeristy, Shahrekord, I.R.Iran *[email protected]
Introduction &Objectives: Vinblastine (by binding to the Tubulin protein and stopping the Microtubules formation) and Doxorubicin (by inhibiting of Topoisomerase II) induce 'programmed cell death' in cancer cells and lead to more effective treatment compare to other natural compounds in a wide range of cancers. The present study is a bioinformatics study of some other natural compounds having the structural similarity with the compounds mentioned above. Materials & Methods: Initially, natural compounds with similar structure to Vincristine and Doxorubicin were prepared from the ZINC database. After getting three-dimensional structure of tubulin (the target of similar structure ligands of Vincristine) and Topoisomerase II proteins (the target of the structurally similar ligand of Doxorubicin) with the access codes of … and 3QX3, respectively, from pdbbank, the binding affinity of the mentioned ligands with mentioned targets was determined by AutoDockTools-1.5.6 software. Finally, pharmacokinetic properties (ADMET) of ligands considered in this study were evaluated by the FAF-Drug4 server. Results: according to the results of docking studies for ligands with similar structure to Vincristine, ZINC000085537024 have the most negative binding energy (-8.18 kcal/mol) ,compared to ZINC000095616067 ،ZINC000085555528 and ZINC000085432544 (-5.25 -4.73 and -2.57 kcal/mol, respectively). Additionally, for the only ligand having the similar structure to doxorubicin (ZINC000003917708) the energy binding of -11.38 kcal/mol was reported. It is worth to mention that pharmacokinetic studies for all compounds showed promising results. Conclusion: regarding to the results of considered compounds, the ligand with similar structure to doxorubicin (ZINC000003917708) and Vincristine (ZINC000085537024) tend to bind with their targets more effectively. Considering the promising results of drug- likeness as well as absorption, distribution, metabolism, excretion and toxicity properties, obtained from pharmacokinetic analysis, they are the appropriate novel candidates for cancer treatment.
Keywords: Cancer, apoptosis, Natural Compounds, Docking, Pharmacokinetic Properties.
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 75348
The effect of piascledine and TGF β1 on chondrogenesis of human adipose- derived stem cells in fibrin and fibrin-alginate scaffolds
Batool Hashemibeni, Mojtaba Esmaeli, Farhad Golshan Iranpour Anatomical Sciences, Medical School, Isfahan University of Medical Sciences
Objective(s): The aim of this study was to compare the chondrogenic induction potential of Piascledine and TGF-β1 on adipose-derived stem cells (ADSCs) in fibrin and fibrin- alginate scaffolds. Materials and Methods: Human subcutaneous adipose tissues were harvested from three patients who were scheduled to undergo liposuction. Isolated ADSCs were proliferated in a culture medium. Then, the cells were seeded in fibrin or fibrin-alginate scaffolds and cultured for 14 days in a chondrogenic medium containing Piascledine, TGF-β1, or both. The rate of cell proliferation and survival was evaluated by using MTT [3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide] assay and the rate of the expression of type II collagen, aggrecan, and type X collagen genes was evaluated by real- time polymerase chain reaction (real-time PCR) method. Results: The MTT results showed that Piascledine is able to enhance the proliferation and survival of ADSCs in fibrin scaffolds in comparison to other groups (P<0.05). Real- time PCR evaluation revealed that the expression of type II collagen was higher in TGF- β1groups, but the expression of aggrecan was higher in TGF-β1 alone or along with Piascledine in fibrin-alginate scaffolds. Furthermore, the expression of type X collagen was lower in Piascledine alone or along with TGF-β1 in fibrin scaffold. Conclusion: Piascledine can enhance the proliferation and differentiation of ADSCs in fibrin scaffolds. Keywords: piascledine, stem cells, chondrogenesis
239 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 75577
Oral Administration of Probiotic Enterococcus Durans Ameliorates Experimental Autoimmune Encephalomyelitis in Mice
Seyed Abdollah Samani 1, kambiz Hassanzadeh 1, 2 *
1- Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran 2- Department of Medical Physiology and Pharmacology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
Introduction & Objectives: Multiple sclerosis (MS) is a myelin-degenerating autoimmune disease in the central nervous system (CNS). Experimental autoimmune encephalomyelitis (EAE), due to its clinical and pathologic features similar to multiple sclerosis, is widely used in many studies of this disease as an effective and similar model. Probiotics, including lactobacilli, are known to induce immunomodulatory activity with promising effects in inflammatory diseases. In this study, we tested the potential of Enterococcus durans and three various strains of lactobacilli (lacto-mix), Including L.rhamnosus, L.casei, and L.plantarum for prevention of EAE features. Methods: C57BL/6 female mice were inoculated with (MOG35-55) / (CFA) to induce EAE. Different groups (five groups: n = 6 in each group) of animals received saline or probiotics. Results: Histopathological studies showed an increase in infiltration of inflammatory cells and destruction of the myelin membrane in the EAE group but a decrease in the probiotic- treated animals. Pro-inflammatory cytokines (IL-17 and IFN-γ) concentration in the supernatant of the brain and spinal cord homogenized tissues showed a significant increase in the EAE compared with the normal saline group. While in the spinal cord tissue there was a decrease in IL-17 in those animals treated with the Lacto-mix and Edu + Lacto- mix and a significant decrease in IFN-γ in those animals that received Edu. In addition, Western blot analysis of MMP-9 and MBP proteins showed a decrease and increase in treatment and EAE groups, compared to the normal control group respectively. Conclusion: Collectively, our data suggest that probiotic Enterococcus durans and lacto- mix had a preventive effect against EAE but further studies are needed to clarify the exact mechanisms and their application in preclinical and clinical trials. KEYWORDS: Multiple sclerosis (MS), Experimental autoimmune encephalomyelitis (EAE), anti-inflammatory, antioxidant, Probiotic, Enterococcus durans
240 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 75644
Examination of the role of genetics in thyroid neoplasm
Roya Hajian*1, Seyed Morteza Javadirad2
*1- MS student of Genetics, Department of Cellular and Molecular Biology, Faculty of Biological Sciences and Technology, University of Isfahan, Isfahan, IR IRAN 2- Assistant Professor at Department of Cellular and Molecular Biology, Faculty of Biological Sciences and Technology, University of Isfahan, Isfahan, IR IRAN
Introduction: Thyroid neoplasm, like other cancers, associates with the lack of control over the growth and development of cells, and death is the outcome of the disease. Thyroid neoplasm is the fifth most common cancer among American women and also the most common endocrine cancer in Iran. Due to the increasing rate of thyroid neoplasm development in recent years, investigating the role of genetics in this neoplasm has been considered. Description: Sequencing analysis of genomic-DNA combined with family history exploring and twins’ studies, altogether implemented the importance of genetics in the incidence of the neoplasm. Mutations of MAPK signaling pathway has been observed in the most of thyroid neoplasm. Also over the past decades, genome-wide association studies (GWAS) have identified and introduced various genes associated with different phenotypes of the thyroid gland neoplasm. Discussion and conclusion: The most frequent mutation of non-medullary thyroid neoplasm is transversion of thymine 1799 to alanine resulting to a mutant kinase named BRAFV600E especially in the case of papillary thyroid neoplasm (PTC). Mutations in RAS oncogene family members are also frequently found in follicular thyroid cancer (FTC). Chromosomal translocations are occurred in thyroid neoplasm such as PPARɣ (30% of FTC) and RET (7% of PTC) translocation. On the other hand, RET proto-oncogene mutation has been observed in the most medullary thyroid neoplasm (MTC), but few RAS mutations occur in this type of thyroid cancer. RET mutations could be inherited somatically or via germ line, but they can emerge as sporadic with autosomal dominant inheritance. Therefore, evaluation of RET hereditary genetic mutation is recommended for all MTC patients regardless of their family history or age.
241 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 75704
Surface Modification of Polycaprolactone Scaffold via Immobilized Carboxymethyl Chitosan for Bone Tissue Engineering Application
Anita Kabirkoohian*1, Shiva Irani1, Hadi Bakhshi2, Behafarid Ghalandari3 1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran 2. Macromolecular Chemistry II, University of Bayreuth, Universitätsstraße 30, Bayreuth, Germany 3. Department of Medical Nanotechnology, Applied Biophotonics Research Center, Islamic Azad University, Tehran, Iran *Corresponding author: Anita Kabirkoohian, Email: [email protected]
Introduction & Objectives: Bone as a mineral tissue is involved in the mechanical protection of organs and the maintenance of mineral homeostasis. Following bone defects caused by degenerative diseases and trauma, many patients are not fully treated. For this purpose, bone tissue engineering with the aim of repairing damaged mineral tissue has been considered with offering new solutions. Synthetic polycaprolactone (PCL) polymer with properties such as biocompatibility, biodegradability can be a good choice as a scaffold manufacturer. However, due to the hydrophobic nature of PCL, cell adhesion is not well performed, which can be remedied by the use of carboxymethyl chitosan (CMC). In this study, CMC was immobilized by chemical method on electrospun PCL scaffolds for bone tissue engineering application. Materials & methods: First, the PCL scaffold was fabricated using electrospinning process. Then, the CMC was chemically immobilized on the PCL scaffold. To confirm the immobilization of CMC, Fourier Transform Infrared Spectroscopy (FTIR) was performed then a scanning electron microscope (SEM) image was taken from the PCL/CMC scaffold. To evaluate the biocompatibility of the scaffold, human mesenchymal stem cells were cultured on the scaffold for 24 h. Finally, Alizarin red staining was performed to evaluate the differentiation of hMSCs toward bone for 7 days. Results: The results of SEM images and FTIR test showed that the immobilized CMC was well on the PCL scaffold. The images of hMSCs next to the scaffold showed that the cells were adapted to the scaffold and had better growth and proliferation on the immobilized scaffolds. Alizarin staining results confirmed the differentiation of hMSCs toward bone. Conclusion: According to the results, this synthetic scaffold can be used as a suitable candidate for bone defects repair in bone tissue engineering. Keywords: Carboxymethyl chitosan; Polycaprolactone; Immobilization; Human mesenchymal stem cells; Bone tissue engineering.
242 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 75723
Reconstruction of the co-expression network for the hippo signaling pathway in glioblastoma
Akram Aminjafari1 1. Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
Introduction & Objectives: The Hippo signaling pathway (HSP), Last known signaling pathway has been identified as a Highly conserved, essential and complex signaling pathway for tumor suppression that coordinates proliferation, differentiation, cell death, cell growth and stemness. Glioblastoma (GBM) the most common malignant brain tumor with unique characteristics of highest invasion, tumor vascularization, heterogeneity, maintaining stem cell-like cells and malignancy rate, often resulting in poor prognoses and high resistance rates to combination therapies. Discovery of genes and signaling pathway associated with such unique hallmarks in GBM using system biology approach provide new targets and improve combination therapies and clinical care. Materials & Methods: In the present study, we conducted a genome-scale co-expression analysis to reconstruct the HSP in GBM. glioblastoma Microarray data analysis was performed using WGCNA package in R software. Results: In conduction of a genome-scale co-expression analysis to reconstruct the HSP in glioblastoma, four key modules were detected through network clustering, and a detailed discussion of two modules containing respectively 15 and 9 over and down-regulated members of HSP was provided. Conclusion: Our results suggest new potential regulatory factors in the HSP. The detected modules also suggest novel genes contributing to GBM. Moreover, differential expression analysis confirmed the differential expression pattern of HSP members and new suggested regulatory factors between tumor and normal samples. These findings can further reveal the importance of HSP in GBM. Keywords: Hippo signaling pathway, glioblastoma, gene co-expression network, WGCNA
243 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 75847
Bioinformatics study of the binding affinity and pharmacological properties of anticancer compounds targeting topoisomeraseI in cancer treatment.
Toktam sheidaeian1*, Massoud Houshmand2 1. Department of Genetics, Department of Biology, High Institute nurdanesh, Meymeh, Isfahan, Iran. 2. Medical Genetic Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
Introduction &Objectives: TopoisomeraseI is one of the important targets of anticancer drugs. This protein plays an important role in DNA repair and replication. Anti-cancer drugs with the ability to inhibit topoisomeraseI apoptosis in cancer cells. One of the important goals in cancer research is using the best and most effective drug to prevent topoisomeraseI function in cancer cells. The present insilico study compares the therapeutic potential anticancer ability of several anticancer drugs for targeting topoisomeraseI. Materials & Methods: Firstly, topoisomerase I-related drugs that were approved was searched in the Drugbank database. Then, swissADMET database was used to evaluate their pharmacokinetic properties. The chemical structures of all compounds were designed using the ChemBioDraw Ultra14.0 software. Then to maximize energy efficiency, they were exported into the HyperChem software package. Results: After analysing the Drugbank database, 3 approved drug combinations Topotecan, Lucanthone and Irinotecan were selected. According to the results of docking studies, Topotecan with Topotecan-TopoisomeraseI complex (with 4/16 kcal / mol energy) as well as better pharmacokinetic properties including higher lipophilicity and non-toxicity were identified as the best drugs for inhibiting topoisomeraseI in this article. Conclusion: Based on the results of the studies and comparison of the results, it can be concluded that among the investigated compounds, Topotecan exhibits better pharmacological properties and would act more effectively in inhibition of topoisomerase I protein and cancer treatment Keywords: Cancer, TopoisomeraseI, Pharmacokinetics, Molecular Docking, Anticancer Drugs
244 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 75853
A large deletion of the BRCA1 gene in a hereditary breast cancer: A case report
Zahra Sadri1*, Mehrdad Zeinalian1,2 1 Ala Cancer Prevention and Control Center, Isfahan, Iran 2 Department of Genetics and Molecular biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract: Approximately 5% to 10% of breast cancers are hereditary, and heterozygous germline BRCA1/2 mutations are responsible for the majority of hereditary breast and ovarian cancers, (especially at age before 50). Medullary carcinoma is a rare subgroup of breast cancers accounting for less than 5% of invasive breast carcinomas. They are most often negative for estrogen and progesterone receptors and HER2 (TNBC). The TNBCs are enriched for germline mutations in BRCA1/2, compared to other subtypes of breast carcinomas. It has been well known that invasive ductal carcinoma with medullary features are associated with germline BRCA1 mutations. About 35% to 60% of BRCA1-associated breast cancers show the medullary features. We report a 33-year-old female patient with triple negative invasive ductal carcinoma, medullary type breast cancer. The patient's mother was affected at age 49 with esophagus cancer and her grandfather had tumor in his liver at age 70. Two grandfather's brothers had stomach and larynx cancers at age 40 and 55 respectively. Although marriage of patient's parents was not consanguineous, her father and his two half-brothers were also affected by unknown primary site (61-year-old), unknown primary site (55-year-old) and stomach (60- year-old) cancers, respectively. MLPA analysis for the patient revealed heterozygote deletion in exons 13 to 15 of BRCA1. Her sister was carrier of this pathogenic mutation but the parents were dead. According to the NCCN guidelines the offer for women with a BRCA mutation who have breast cancer is screening of residual breast tissue with annual breast MRI and mammography should continue. Salpingo-oophorectomy can be offered 35 to 40 years, and upon completion of child bearing. Transvaginal ultrasound for ovarian cancer may be considered, starting at age 30–35 years. Serum CA-125 is also an additional ovarian screening test. Keywords: medullary carcinoma, BRCA1, exon deletion, TNBC
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 76787
Evaluation of the role of rs3789327 polymorphism in increased risk of multiple sclerosis
Zeynab Ghafelynezhad1, Abbas Moridnia2*, Fatemeh Bossaghzadeh1 1. Department of Biology, Dezful Branch, Islamic Azad University, Dezful, Iran. 2. Department of Genetics and Molecular Biology, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran Introduction: Multiple sclerosis (MS) is an inflammatory demyelinating and potentially disabling disease of the central nervous system with varied clinical appearances and heterogeneous histopathological features. Today, it has been found that alterations in single nucleotide sequences (polymorphisms) are associated with predisposing or resistance to diseases. The past studies have shown that genetic alterations in key regulators of circadian rhythm, such as the ARNTL gene, may increase the risk of MS. The aim of this study was to evaluate the frequency and association of rs3789327 polymorphism in ARNTL gene with multiple sclerosis in MS patients compared to healthy individuals in Khuzestan population. Materials and Methods: Sixty patients with multiple sclerosis referred to the MS Community Center of Isfahan as case group and 50 healthy subjects as control group was selected. Then, 3 cc whole blood were taken and shed in EDTA tubes and were stored in - 20 ° C until DNA extractions. The genotyping for rs3789327 polymorphism was evaluated by PCR-Sequencing. Results: In this study, AA genotype was 20% in patients and 27% in the control group. AG genotype in patients was 35% and in healthy subjects 40% and GG genotype of this polymorphism was 45% in patients and 33% in healthy controls. The prevalence none of the genotypes were statistically significant in the two groups (P> 0.05). Conclusion: The results showed that there is no significant relationship between rs3789327 polymorphism in the ARNTL gene and susceptibility to multiple sclerosis in studying population. Keywords: Multiple Sclerosis, ARNTL gene, Polymorphism
246 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 77654
Bioinformatics study of hsa-miR-99a and LNCRNA ANRIL targeting pathways in acute lymphoblastic leukemia
Marziyeh Akbari1*, Massoud Houshmand2
1. Department of Genetics, Department of Biology, High Institute nurdanesh, Meymeh, Isfahan, Iran. 2. Medical Genetic Department, National Institute of Genetic Engineering and Biotechnology, Tehran,Iran. Introduction & Objectives: Acute lymphoblastic leukemia is the most common cancer in children. Since the etiology of hematologic diseases is unknown, it has been attempted to investigate regulatory and functional genes such as LNCRNAs. ANRIL is one of the LNCRNAs that its expression, regulates its important adjacent genes, CDKN2A and CDKN2B (coding tumor suppressor proteins). The aim of this study was to analyze the bioinformatics pathway of LNCRNA ANRIL (CDKN2B-AS1) by targeting its specific microRNA in leukemia cancer. Materials & Methods: At first, studies of different articles revealed that ANRIL LNCRNA causes cancer by targeting different microRNAs in different cancers. hsa-miR- 99a is one of the microRNAs that causes tumor growth through suppression by ANRIL. By confirming the role of hsa-miR-99a in leukemia cancer through the phenomiR database, this microRNA was analyzed by the miRbase database. Then, a variety of hsa-miR-99a target genes were searched in the MiRWalk database and the most relevant signal pathways from the KEGG database to the target genes and hsa-miR-99a were analyzed by using the DAVID bioinformatics database. Results: Results from the KEGG and phenomiR database indicatied that hsa-miR-99a regulates tumor suppressor genes by targeting CDKN2A and CDKN2B genes. On the other hand, hsa-miR-99a has been shown to be downregulated in leukemia, and by less targeting these two genes, it can cause cancer. As a result, ANRIL indirectly causes cancer by suppressing aging by the action of CDKN2A and CDKN2B on metastatic development. Conclusion: Bioinformatics of cancer pathways indicatied that ANRIL LNCRNA inhibited tumor suppressor genes by suppressing hsa-miR-99a. As a result, ANRIL LNCRNA is an oncogene gene and hsa-miR-99a is a tumor suppressor mir in the leukemia cancer pathway. Keywords: leukemia, LNCRNA ANRIL, hsa-miR-99a , CDKN2A , CDKN2B
247 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 77659
S14161 as a novel small molecule promoted the apoptosis in A549 cancer cells
Sima Orouei1*, Maliheh Entezari2, Mehrdad Hashemi2 1. MSc. Student, Department of Genetic Science, Medical Science Branch, Islamic Azad University, Tehran, Iran. 2. Department of Genetic, Faculty of Advanced Sciences and Technology, Tehran Medical Science, Islamic Azad University, Tehran, Iran
Background and objectives: Lung cancer is the second most widely recognized cancer in the world that causes the highest cancer deaths in men. Since most modern cancer therapies are ineffective and have many side effects, effective treatment is therefore needed. Small molecular cases have been identified today in the treatment of cancer with little side effects. One of these small molecules is the S14161 that is PI3K inhibitor. Therefore, in the present study, the effect of apoptotic S14161 small molecule in A549 cell line was investigated. Material and Methods: Cell cytotoxicity of S14161 small molecule at concentrations of 2, 5, 10, 20, and 40 (mM) was investigated by MTT technique and Bcl2, Bax gene expression was measured by real-time PCR method. One-way ANOVA was used for statistical analysis. Results: In the MTT assay, the IC50 of S14161 small molecule was determined in the A549 cell line, 10 Mm, which showed a dose-dependent cytotoxic effect (p <0.05). The results of qRT-PCR showed an increase in Bax gene expression and a decrease in Bcl2 gene expression in A549 cells within 24-hour treatment with S14161 small molecule, which induces apoptosis. Conclusion: S14161 small molecule by inducing PI3K/AKT pathway can induce apoptosis in A549 cells and can be a good candidate for cancer treatment. Keywords: A549, PI3K, Apoptosis, S14161
248 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 78418
Evaluation of GSK3β gene expression alteration during adipogenesis in human mesenchymal stem cells
Marjan Nourigorji 1,2, Samaneh Palizban 1,2, Mehdi Dianatpour 2
1. Student research committee, Shiraz University of medical sciences, Shiraz, Iran. 2. Department of Medical Genetics, shiraz university of medical sciences, Shiraz, Iran. Objectives & introduction: Obesity is a risk factor responsible for the development of several metabolic diseases. Physiologically, obesity can cause by adipocyte hypertrophy and hyperplasia. Adipogenesis is the process resulting in adipose tissue hyperplasia. Different signaling pathways, such as wnt/βcatenin pathway, control the differentiation of pre-adipocytes into adipocytes. GSK3β plays a vital role in the canonical WNT signaling pathway. It was shown that GSK3β inactivation or -catenin activation block the adipogenesis inducing mechanical signals. It has not been known yet how GSK3β gene expression changes during adipogenesis, which in this case,𝛽𝛽 discovering it can help to find more effective inhibitors that are well capable of preventing and treating obesity. Materials & methods: In this study, human mesenchymal stem cells were isolated from abdominal fat and differentiated into adipocyte under adipogenic medium. Adipogenesis was confirmed by examining the expression of PPARγ and C/EBPα genes and oil red o staining. Then the expression of PPARγ, C/EBPα and GSK3β genes at 1 day, 5 days and 10 days after differentiation was evaluated in comparison to control (undifferentiated cells) by using quantitative real-time polymerase chain reaction(qRT-PCR). Gene expression data were analyzed by one-way ANOVA in graphpad prism 8 software and p-value 0.05 was considered significant. Results: Adipocyte differentiation has been confirmed by the results of oil red o staining≤ and PPARγ and C/EBPα genes expression evaluation. This study showed that GSK3β gene expression during adipogenesis was significantly increased by p-value 0.05. The highest GSK3β gene expression was observed at five days after differentiation. Conclusion: since GSK3β could be regarded as a potential therapeutic≤ target to modulate proliferation and differentiation of adipocyte precursors, the results of the present study can help to the discovery of more effective GSK3β inhibitors that can prevent and treat obesity. Keywords: Obesity, Adipogenesis, GSK3β, Gene Expression
249 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 78710
Stem cells treatment for HIV
Mohadeseh Ghorbani Masouleh1, farzaneh jabbari2, Reza Salarian1
1. Department of Biomedical engineering, Maziar Higher Education Institute, Royan, Iran 2. biomedical engineering, Materials and Energy Research Center
Introduction: HIV infects specific types of cells in the immune system. For infecting the cells this virus must bind to a specific receptor on the cell’s surface. Our bodies produce new immune cells every day from stem cell reservoirs in bone marrow and these cells play a specific role in curing bacterial and viral infections. Description: One area of research for HIV is to look at how stem cell therapies might help. Scientists found that a stem cell therapy could effectively arm the immune system with a special defense against HIV. It is also hoped that it can improve their life expectancy, particularly when antiviral drugs are not successful anymore. Discussion and conclusion: it is possible to derive patient-specific stem cells from HIV- infected individuals, differentiate them into bone marrow stem cells, and knock-out the CCR5 protein, rendering them resistant to HIV infection and these cells are available for transplant into the HIV infected individual. The new cells are so non-sensitive to HIV infection and would replace the individual’s HIV infected cells. Keywords: stem cells, HIV
250 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 79154
Synthesis and fabrication of quercetin-loaded electrospun nanofibrous mats with antioxidant activity for proliferation and stemness preservation of human mesenchymal stem cells
Sara Nourozi Dizach1, Mehdi Dadashpour2, Akram Mohammadi3, Nosratollah Zarghami*4 1. Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran 2. Department of Medical Biotechnology, Faculty of Advance Medical Science, Tabriz University of Medical Science, Tabriz, Iran 3. Department of Biology, Faculty of Science, Tabriz branch Islamic Azad University, Tabriz, Iran 4. Department of Clinical Biochemistry, faculty of Medicine, Tabriz University of Medical Science, Tabriz, Iran
Introduction: An ideal biomaterial in regenerative medicine should be able to regulate the stem cell proliferation without the loss of its pluripotency. quercetin is one of the most important flavone with a wide spectrum of biological functions including anti- inflammatory and anti-oxidant properties. The present study describes the influence of Que-loaded electrospun nanofibers on the regulation of proliferation and stemness preservation of Mesenchymal stem cells (MSCs). Methods: For this purpose, Que -loaded poly (e-caprolactone)/poly (ethylene glycol) (PCL/PEG) electrospun nanofibers were formed via electrospinning procedure and the effective fabrication of these bioactive nanofibers was confirmed by field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy. MSCs were seeded on the electrospun nanofibers and their morphology, viability, and stemness expression were analyzed using FE-SEM, MTT, and qPCR assays after one weeks of incubation, respectively. Results: The results show that MSCs display better adhesion and significantly increased viability on the Que -loaded PCL/PEG nanofibrous mats in relative to the PCL/PEG nanofibers and tissue culture polystyrene. The greater viability of MSCs on Que based electrospun nanofibers was further confirmed by higher expression levels of stemness markers Sox-2 and Nanog. Conclusions: These results reveal that Que-loaded PCL/PEG electrospun nanofibrous can be used to improve cell adhesion and proliferation while synchronously preserving the stemness of MSCs, thus representing a hopeful potential for application in stem cell therapy strategies. Keywords: quercetin, Electrospun nanofibers, Stem cells, Pluripotency
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 79232
Warton's jelly explant is a source of EGF and bFGF growth factors
kaveh khazaeel1, Fereshteh Nejaddehbashi2*, Mahmoud Orazizadeh2&3, fatemeh khademi moghadam 4 1. Department of basic sciences, Faculty of veterinary medicine, Shahid chamran university of Ahvaz, Iran 2. Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 3. Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 4. Graduated Student, Faculty of science, Shahid Chamran University of Ahvaz, Iran Background: Warton's jelly is a source of mesenchymal stem cells and growth factors, for this reason Wharton's Jelly-Mesenchymal stem cells and release medium from explant can be considered as an appropriate source for regenerative medicine. Materials and methods: Warton's jelly explant were kept in DMEM low glucose for 10 days. After 4, 6, 8 and 10 days’ medium around explant were collected and kept in -20c°. The assays of growth factors were carried out with the use of Quntikine human EGF and bFGF ELISA commercial kits, together with SDS, polyacrylamide gel electrophoresis of tissue extracts. Growth factor such as EGF and bFGF were detected in Wharton's jelly. The amounts of these factors per gram of tissue vary from about 900 pg bFGF, to about 90 ng in EGF. The effect of this culture medium were analyzed on proliferation of HUVEC cells in compared with DMEM low glucose with MTT procedure for 1,3 and 5 days. Results: The results of this study showed that Warton's jelly explant culture medium could release EGF and bFGF into medium after 4 days. This medium could increase proliferation of HUVEC cells. Conclusion: Wharton's jelly explant culture medium after 4 days can be a suitable medium for cell culture. This method is easy and cost consuming for cell culture in regenerative medicine. Keywords: Wharton's jelly, explant method, EGF, bFGF, cell culture medium
252 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 79996
In Silico Molecular Docking of HSPA2 with 4HNE in Human sperm
Sara Ahmadpour1, Zahra Rezvani1 1. Biotechnology Department, Faculty of Chemistry, University of Kashan, Kashan
Abstract: Polyunsaturated fatty acids are susceptible to peroxidation and they yield various degradation products, including the main α, β-unsaturated hydroxyalkenal, 4-hydroxy-2,3- trans-nonenal (HNE) in oxidative stress. The molecule is highly reactive and able to react readily with various cellular components, such as DNA, proteins and other molecules containing nucleophilic thiol or amino groups. Protein-HNE adduct formation can lead to alterations in the normal functioning and modified activity of various proteins. HSPA2 is a testis-enriched member of the HSP70 family of chaperones, which is primarily responsible for preventing the aggregation of misfolded proteins and for fulfilling secondary roles in the transmembrane transport of client proteins and the formation of multimeric protein complexes. HSPA2 was present in the acrosomal domain of human spermatozoa as a major component of molecular complexes, in close association with just two other proteins, sperm adhesion molecule 1 (SPAM1) and arylsulfatase A (ARSA), both of which that have previously been implicated in sperm-egg interaction. HSPA2 is key target for 4HNE adduction reactions and that its chaperoning activity is particularly susceptible to such an insult. 4HNE adduction directly leads to a loss of HSPA2 chaperone activity and a subsequent perturbation of zona pellucida protein complex assembly. Aim: This study is to understand Which region of HSPA2 is bound to the 4HNE? Method: Cristal structure of human HSPA2 was ducked against 4HNE in a run by hex 8 and the interactions between them were analyzed in ligplus v.1.4.5 Results: The results showed that HSPA2/4HNE complex number of hydrogen bondages is 3 with binding energy -4.2. According to results 4HNE interact with HSPA2 but in a region other than our selective area. Keywords: lipid peroxidation, HSPA2, 4HNE, docking, infertility
253 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 80185
Metformin- mesoporous silica nanoparticles loaded electrospun thermo- responsive copolymer for targeted ovarian cancer therapy
Shadi Samadzadeh1, Mehdi Dadashpour2,Yones Pilehvar-Soltanahmadi3, Hanieh Mousazadeh4, Nosratollah Zarghami5*
1. Department of Chemistry, Faculty of Science, Tabriz Branch- Islamic Azad University, Tabriz, Iran 2. Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran 3. Cellular and Molecular Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran 4. Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran 5. Department of Clinical Biochemistry and Laboratory Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Background: Breast cancer is one of the most common malignancies in women, and its incidence has increased in recent year. Although Metformin (Met) has good biocompatibility, its clinical applicability is limited by poor solubility in water, low stability, and a short half-life in the circulation. Nanocarriers have attracted broad attention in cancer therapy because of their ability to carry drugs preferentially into cancer tissue, but their application is still limited due to the systemic toxicity and low delivery efficacy of intravenously delivered chemotherapeutics. In this study, we develop a localized drug delivery device with combination of a mesoporous silica nanoparticles and implantable polymeric nanofibers. Methods: The fibers are produced by electrospinning copolymers of N-isopropylacrylamide (NIPAAm) and N-hydroxymethylacrylamide (HMAAm). The OH groups of HMAAm are subsequently crosslinked by thermal curing. The copolymers were successfully fabricated into a well- defined nanofibrous structure. Moreover, the ‘on–off’ switchable release of MET from the crosslinked NFs was observed. Cytotoxic assays were evaluated in SKOV3 cells treated with the MTT assay. Also, real-time polymerase chain reaction (Real-Time PCR) was used to determine the gene expression levels of hTERT. Results: In vitro cytotoxicity assay using MTT revealed that the MET and MET/MSN@P(NIPAM- co-MAA) nanofibers exhibited a dose- and time- dependent cytotoxic e ect against Skov3 cells. qPCR findings showed that the MET/MSN@P(NIPAM-co-MAA) nanofibers also down-regulated the expression levels of hTERT at all used concentrations compared with ffthe drugs used alone after 28 h treatment. Conclusion: Our results reveled that incorporation of smart properties into NFs takes advantage of their extremely large surface area and porosity and is expected to provide a simple platform for on–off drug delivery. Also, these results indicate that MET/MSN@P(NIPAM-co-MAA) nanofibers are biocompatible and effectively deliver drugs to the tumors and suppress tumor growth.
Keywords: Thermoresponsive nanofiber, N-isopropylacrylamide (NIPAAm), Drug delivery system, Electrospinning, Metformin, Mesoporous silica nanoparticles
254
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 80819
Investigation of the myelin recovery and expression changes of miR-219 and miR-155-3p following the administration of hydroxychloroquine in multiple sclerosis-induced model
Fatemeh Mazloumfard1*, Seyed-Mehdi Eftekhari 2, Mina Mirian3, and Mehdi Aliomrani1
1. Department of Toxicology and Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran. 2. Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran. 3. Department of Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
Introduction and Objectives: Multiple sclerosis (MS) is an autoimmune inflammatory disease of the central nervous system which causes demyelination and axonal loss. Based on the previous studies hydroxychloroquine (HCQ) has anti-inflammatory properties and can be a good candidate for the treatment of MS. Moreover, recent studies indicated the role of microRNAs as a biomarker in the diagnosis and treatment of MS. This study aimed to investigate the effect of HCQ on the expression of miR-219 and miR-155-3p involved in the myelination process by cuprizone-induced model. Materials & Methods: MS model was induced by the administration of cuprizone pellets for 5 weeks (demyelination phase) following normal feeding for one week (remyelination phase). In this experimental study, forty-six C57BL/6 mice were randomly divided into five groups and kept under circumstances in vivarium of Isfahan University of Medical Sciences, control group received normal food pellets for 5 weeks, cuprizone group received cuprizone pellets for 5 weeks, treated groups of mice that received cuprizone pellets and drinking water containing HCQ in three doses of 2.5, 10 and 100 mg/kg during the demyelination phase. In the sixth week, all groups were given normal food pellets and tap drinking water to initiate the remyelination phase. At the end of each phase, half of the mice in each group were perfused, and their brains were removed. The expression levels of miR-219 and miR-155-3p were evaluated by quantitative Real-Time PCR from the posterior part of the brain. Results: In this study, the reduction of miR-219 expression and increment of miR-155-3p expression was observed in the cuprizone group as compared to the control group (p < 0.001). A significant increment of miR-219 expression and reduction of miR-155-3p expression was observed in the animals treated with 100 mg/kg of HCQ when compared with the control group (p < 0.0001). There is no significant difference between the cuprizone group and the group treated with 2.5 mg/kg of HCQ. Conclusion: Based on the obtained results of this study, HCQ could enhance myelination process by targeting miR-155-3p down-regulation more than miR-219 up-regulation so it could be a suitable candidate to suppress the demyelination during MS. Keywords: miR-155-3p, miR-219, Hydroxychloroquine, Multiple sclerosis, Myelination, Cuprizone
255
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 81022
Cervical ectopic pregnancy: one cause of first trimester pregnancy bleeding (a case report)
Farahnaz Mardanian1, Somayeh Khanjani2, Farahnaz Moradgholi3 1. Department of Obstetrics and Gynecology, School of Medicine, Isfahan University of Medical Sciences. 2. Department of Obstetrics and Gynecology, School of Medicine, Isfahan University of Medical Sciences. 3. Beheshti Hospital,Isfahan,Iran.
Back ground: Cervical pregnancy is a rare type of ectopic pregnancy that can be associated with significant hemorrhage and loss of fertility. (1) Its sonographic findings can be difficult to distinguish from normal pregnancy or an abortion in progress. (3) Most of the cervical pregnancies have been reported in patients with a history of vacuum curettage or caesarean section.(2) Given its rarity, most effective treatment protocols are not well established.(1) Case: A 30-year-old woman (Gravida4, Para2 Abortion 1) presented with history of painless vaginal bleeding. Her obstetric history included tow cesarean section and one curettage after previous abortion. Transvaginal ultrasonography showed 11week gestational sac measuring 52mm with fetal pole without cardiac activity, and with diagnosis of missed abortion, misoprostol was prescribed. Because of continued vaginal bleeding and no response to misoprostol, D&C was performed and due to sever vaginal bleeding during D&C that wasn’t controlled with Foley catheter balloon compression, total abdominal hysterectomy was done. The diagnosis was clarified by pathological examination that showed a cervical ectopic pregnancy. Conclusion: Cervical pregnancy is a rare form of ectopic pregnancy in which the blastocyst implants below the internal cervical os. (4) Risk factors include previous dilatation and curettage, intrauterine device use, previous cesarean section, congenital structural anomalies, large uterine fibroids, infection and inflammation, in vitro fertilization and advanced maternal age. (3) Nowadays it is still difficult to diagnose and challenging to treat (4). Treatment options depend on gestational age and the woman’s hemodynamic stability, usually involving more than one approach to minimize risks of massive hemorrhage and maintain fertility. These include D&C followed by a Foley catheter balloon compression of placental bed, uterine artery embolization followed by, or methotrexate, with or without subsequent D&C. Most second and third trimester cervical pregnancies are treated with hysterectomy. The keys to successful management are
an early diagnosis, planned intervention and good control of bleeding.
256
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 82156
Copper compounds as anti-colorectal cancer agents
Fatemeh Maghool, Mohammad Hassan Emami Poursina Hakim Digestive Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
Introduction: One-third of cancer related deaths are due to the behavioral risk factors and dietary. The trace element copper (Cu) is vital for numerous biological processes. Recently, copper-related mechanisms have been suggested as preventive and therapeutic targets in cancer. Therefore, the aim of this review was to verify the therapeutic benefits of copper in colorectal cancer (CRC) treatment. Description: We searched relevant articles between 2000-2020, in electronic databases MEDLINE, COCHRANE and EMBASE. Only relevant English articles were included. It has reported that CRC patients with drug-resistant tumors have remarkably more serum copper respect to the drug-sensitive ones. Cu concentration has been proven extremely increased in cancer tissues for promoting cancer development. Angiogenesis and the neovascularization are the main processes in tumor growth and metastasis that require Cu as an essential co-factor. Proliferation and migration of endothelial cells are stimulated by this element. Some proangiogenic mediators can be decreased using the anti-copper drugs. Moreover, the role of Cu in BRAF signaling modulation is particularly relevant in CRC. Most of the BRAFV600E mutant CRC patients have respectively extremely more serum copper than healthy and do not respond to BRAF inhibition monotherapy. Therefore, as an adjuvant therapy, copper chelation therapy can possibly be proposed in BRAFV600E CRC patients. In addition, more sensitivity to proteasome inhibition has been reported in cancer cells respect to the normal cells. Since proteasome inhibition has lower toxicity, certain classes of organic compounds can be used to target copper in cancer cells that result in greatly effective and selective cancer killing that avoids toxicity. Discussion and conclusion: Taken together, optimize and develop more potent and specific anticancer copper ligands may represent a promising therapeutic strategy in colorectal cancer therapy.
257 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 82335
Association of Protease Enzymes with Cancer
Azadeh Teimury*, Zohreh Zahraei
Department of Cell and Molecular Biology, Faculty of Chemistry, University of Kashan, Kashan, I.R. Iran Introduction: Mortality due to cancer has increased, and much research has been done on this issue. Proteases are enzymes that break down peptide bonds in proteins, constitute about 3% of the vertebrate genome, and constitute a large group of enzymes that can be identified in all areas of life. These enzymes are classified into six groups: serine protease, cysteine protease, aspartic protease, threonine protease, metalloprotease, and glutamate protease. Description: The role of proteases is not limited to the breakdown of proteins and has various functions, including modulating enzyme activity, regulating membrane function, altering receptor channel properties. In this review, based on studies and research, we first introduce the types of protease enzymes, their general structure, types, and especially their inhibitors. We briefly discuss proteases' role in various diseases such as neurological, cardiovascular, pulmonary diseases. Furthermore, we individually examine the association of each of them, especially serine protease, cysteine protease, and metalloproteases with cancer. These three enzymes have been studied more than other proteases. Discussion and conclusion: Form various studies and research, it will be concluded that the progression of many cancers is associated with increased tumor growth, and invasion and proteases are directly related to this. It has been shown that some proteases' inhibitors can reduce the invasive and metastatic capabilities of tumor cells. Since some protease activity has been shown to allow cancer cells to metastasize to surrounding tissues, they can be used as therapeutic targets.
258 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 82529
Omega 3 acid and wound healing in diabetes
Cheshmeh Habibi Roudsary1*, Farzaneh Jabbari2, Reza Salarian3 1. Department of Biomedical engineering (Biomaterial), Maziar Higher Education Institute, Royan Iran 2. PhD Biomedical engineering (Biomaterial), Nanotechnology and Advance Material Department, Materials and Energy Research Center (MERC), Alborz Iran 3. Department of Biomedical Engineering (Biomaterial ), Maziar Higher Education Institute, Royan Iran
Background and objective: Omega-3 fatty acids are one of the fats in the human body that are abundant in fish or fish oil and have potential effects in modulating various diseases, especially diabetes mellitus. Wound healing failure is one of the most common chronic complications. Diabetes is a disease, which has many contributing factors in the onset of this failure. Due to the anti-inflammatory and vascular effects of fish oil, this oil may modulate many of the potential mechanisms involved in wound healing and accelerate wound healing. Search method: In the context of this overview, 24 articles were used between the years 2010 through the end of 2018 using the Keywords of PubMed, Google scholar search engines. Results: Replacing unsaturated fatty acids in the diet instead of saturated fatty acids improves insulin function. Many studies have also confirmed the positive effects of using unsaturated fatty acids such as omega-3 in the diet of patients with inflammatory diseases such as type 2 diabetes. Other studies have shown that the concentration of some fatty acids can regulate the body's inflammatory responses and is effective in preventing type 2 diabetes. Conclusion: Researchers have concluded that the proper use of unsaturated fatty acids and vitamin D can modulate the inflammatory effects in patients with diabetes and control the progression of the disease. Keywords: Omega-3, Fatty acids, Diabetes
259 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 82748
The effects of addition of vitamin B12 in semen cryopreservation medium on the post-thaw sperm function
Hosseinabadi F1, Jenabi M1, Ghafarizadeh AA1, Yazdanikhah S1
1. Arak Infertility Center, ACECR, Arak, Iran
Introduction & Objectives: Cryopreservation may lead to deleterious changes of sperm structure and function (such as decreasing Motility, viability and increasing DNA damage). Vitamin B12 has antioxidative properties and can protect DNA from free radicals. Vitamin B12 also plays important role in DNA methylation. Recent studies revealed that vitamin B12 may modulate oxidative stress responses through enhancing methionine synthase activity that is linked to glutathione synthesis—a major intracellular antioxidant. In addition, vitamin B12 might act directly as a scavenger of ROS. The objective of this study was to investigate the effects of vitamin B12 supplementation in human sperm quality during the freezing-thawing process. Materials & Methods: Thirty Semen samples were collected from men with normal sperm parameters and were assessed according to World Health Organization guidelines. Following semen liquefaction, each sample was diluted (v/v) with Cryosperm that contained different concentrations of vitamin B12 (.5, 1, 2, 2.5 mg/ml). The diluted semen samples were drawn into straws and froze. After thawing, all samples were evaluated for motility and viability. Based on results, 2 mg/ml was considered as the optimal concentration of vitamin B12 for evaluating sperm DNA fragmentation. The data analyzed by one-way ANOVA and Tukey post-test (P<0.05). Results: The results showed that 2 mg/ml vitamin B12 significantly increased motility and viability post-thawing compared to the control (p<0.05). Also the concentration of 2 mg/mL vitamin B12 decreased DNA fragmentation compared to the control. Conclusion: The increased motility, viability sperm were probably due to increasing antioxidant status by increasing of glutathione peroxidase activities, which would give protection against spontaneous lipid peroxidation. It also leads to decreasing of ROS and DNA fragmentation. The present study showed that vitamin B12 supplemented freezing media can improve sperm quality after freezing/thawing. Keywords: cryopreservation, vitamin B12, DNA fragmentation
260 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 82852
Investigating of cyanidin binding behavior upon interaction with human serum albumin and human transferrin: biophysical and molecular docking approaches
Sattar khashkhashi moghadam1 , jamshidkhan chamani*1
1. Department of biology , Faculty of Sciences , Mashhad branch , Islamic azad university , Mashhad , Iran Introduction: Cyanidin, a compound found in fruits, is the derivative from flavonoid. due to its antioxidant property, cyanidin help prevent a variety of fee oxygen radical related diseases, including cancer, diabetes, asthma and cardiovascular. Human serum albumin is the most abundant protein in the circulation system. Human transferrin is responsible for both exogenous and endogenous substance and donates iron to cells through interaction with a specific membrane receptor. The aims of this study is how cyanidin affected HSA and HTF by using biophysical methods. Materials and methods: The fluorescence measurements can provide some information about the binding constant, binding mechanism of drug protein interaction. RLS has been utilize to study of the shape, size and aggregation of biological and chemical species. Molecular docking was performed to predict the binding site location of CYA on HSA and HTF. Result and discussion: The fluorescence emission intensity of HSA and HTF reduced gradually upon interaction with cyanidin. This quenching induces information about complex forming and drug binding interaction. The RLS intensities of HSA_CYA and HTF _CYA increased with the increasing the concentration of the drug concentration. it can be deuced that the occurrence of an interaction between CYA and proteins has led to production of larger particles and consequently enhanced the RLS signals. The best molecular docking results for both HSA and HTF demonstrated that the 425GLU and 109ASN of HSA interacted with cyanidin through hydrogen bonds and 15GLU ,292ASP, 124ARG and 189SER of HTF were the most sensitive residue in this interface. Conclusion: In this study we determined the binding behavior of cyanidin upon interaction with HSA and HTF by using the biophysical methods and molecular docking. The calculated binding mechanism suggests a stable and possibly biologically relevant interaction. Keywords: Cyanidin, Human serum albumin, Human transferrin, Spectroscopy, molecular docking
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 82929
Peripheral Blood or Bone Marrow stem cells?
Mahdis Ameri ¹*, Dr. Rahmatollah Tavakoli 1, Dr. Behzad Nourian 1
1- Department of Medical Engineering, Faculty of Engineering, Maziar High Education Institute, Royan, Iran
Introduction: Over the past decade, the use of peripheral blood stem cells has increased, and now about 75% of unrelated living donor transplants are performed using peripheral blood stem cells without supportive data in the unrelated donor setting. This trend is called into question by results of a large phase III randomized multicenter trial showing similar outcomes of transplant procedures with bone marrow and peripheral blood stem cells from unrelated donors. Although use of peripheral blood stem cells resulted in better engraftment, the rates of chronic and more extensive graft-vs-host disease were higher. Description: In the context of this overview, 18articles were used between the years 2010 through the end of 2018 using the Keywords of PubMed, Google scholar search engines. Discussion and conclusion: Peripheral blood stem cells are currently being used by many centers as a source of adult stem cells because clinical trials of this strategy in related donors have shown lower relapse rates, better engraftment, and increased survival in patients with advanced leukemia and other blood diseases compared with the use of bone marrow stem cells. Peripheral blood stem cells (PBSC) collected after mobilization with granulocyte colony stimulating factor (G-CSF) contain a larger number of CD34-positive (CD34) progenitors and total cells than bone marrow. These observations led to the hypothesis that transplantation of PBSC would lead to lower mortality compared to transplantation of marrow. In addition, PBSC grafts have a higher T cell content, predicting a possibly more powerful anti-leukemia effect. However, the higher T cell content of PBSC may also lead to increased incidence and severity of acute and chronic graft-versus-host disease (GVHD).
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 83041
How arginine crosses across the prokaryotic and eukaryotic membranes: a mechanistic study by umbrella sampling approach
Maral Afshinpour and Hamid Mahdiuni Bioinformatics Lab., Department of Biology, School of Sciences, Razi University, Kermanshah, Iran
Abstract: Arginine, as a semiessential amino acid, plays a crucial role in various cellular functions. Both normal and cancerous cells are required arginine for their cellular physiology. A great deal of the arginine is transported via a type of membrane transporter called cationic amino acid transporter (CAT) from the extracellular to cytoplasmic side of the cells. This study is purposed at investigating the key amino acids during arginine transportation through prokaryotic and eukaryotic CAT proteins. The structure coordinates of p-CAT is obtained from RCSB (PDB Id 6F34). The e-CAT structure was modeled using MODELLER software based on p-CAT as template. Since the CATs are membrane proteins, appropriate lipid bilayers were built around the target proteins by using CHARMM-GUI web server. Thereafter, MD simulations were carried out for prokaryotic and eukaryotic CAT for 600 and 1000 ns, respectively. The umbrella sampling approach was utilized for calculation of free energy differences of arginine transport through each CAT. Based on the potential of mean force (PMF) analyses, arginine binds to the prokaryotic CAT more tightly than the eukaryotic one. Hence, it seems that arginine passes across the e-CAT more comfortable than the p-CAT. In addition, H-bond analysis showed that the residues Asp237, Glu115, Ser321, Glu245, Lys19 in p-CAT and the residues Asp270, Glu185, Ser22, Asp20, Arg27 in e-CAT are the main contributors in interaction with arginine. The findings obtained here at molecular level, might shed light on better understanding of the actual mechanism(s) by which arginine permeate through the membranes of the prokaryotic and eukaryotic cells. Keywords: Cationic amino acid transporter; Molecular dynamics simulation; Umbrella sampling
263 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 83117
Advanced targeted therapy in cancer: nanostructured carriers as innovative tools for cancer diagnosis and therapy
Maryam Zolghadrnasab1, Abbas Farmany2
1. Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Shahrake Pajouhesh, 15th km Tehran-Karaj Highway, Tehran, Iran. 2. Dental Research Center, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran Introduction: Cancer is the second leading reason of death worldwide. This disease is caused by damage or mutations induced by environmental or hereditary factors in the genetic material of cells. Normal cells damage by traditional chemotherapeutic drugs as well as the growth of multidrug resistance and cellular toxicity, reinforce the need to find new targeted effective treatments based on the changes in tumor cell molecular biology. Description: Here, we reviewed the most important nanomaterials currently used in clinical applications and introduced new cancer diagnosis and therapy. In continue, we explored active and passive nanocarriers targeting. Nowadays, nanomedicine is one of the most innovative and advanced areas of biomedical research in targeted therapies. Nanodrugs block biological transduction pathways and specific cancer proteins to cause cancer cell death via apoptosis and immune system activation or directly deliver chemotherapeutic agents to cancer cells which mitigating unwanted side effects. Discussion and conclusion: At this time, several materials and formulating procedures are used for nanodrug manufacturing such as inorganic and organic nanoparticles. Many nanodrugs have already been approved for cancer diagnosis and therapy by the FDA and the European Medicines Agency (EMA). Some of nanodrugs are currently undergoing clinical trial evaluation and are likely to enter the market in the near future.
264 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 83265
Effect of inhibition of miR-21 on lncRNA of tumor suppressor gene role in MCF-7 cells
Samaneh Nejaddehghan1*, Seyed Jalal Zargar1, Mana Oloomi 2 1. Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran 2. Molecular Biology Unit, Pasteur Institute of Iran, Tehran, Iran 3. National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
Abstract: MiR-21 upregulates and this molecule as oncomiR causes survival of cancerous cell and decrease of apoptosis. For miR-21, many macromolecules have been found as targets like components of signaling pathways, DNA repair system and BCL-2 family influencing on many processes such as proliferation, metastasis, and progress of cancer. Based on comprehensive bioinformatics studies: co-lncRNA, Enrichment analysis, Gene Ontology, KEGG, two lncRNAs with two different tumor suppressor and oncogenic roles were considered. In order to inhibition of miR-21, the cells were transfected by anti-miR-21 and due to more confirmation of induced apoptosis, expression of genes non-coding RNAs and expression of two important apoptotic molecules BAX, and P53 were scrutinized by RT- qPCR. Assessment of apoptosis using MTT was done as well. Results showed that after transfection, miR-21 was down-regulated while lncRNA of tumor suppressor role was up- regulated. In this case, negative relationship between miR-21 and lncRNA of tumor suppressor was confirmed. Keywords: MiR-21, lncRNA, apoptosis, breast Cancer
265 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 83267
Intravascular brachytherapy with Iridium 192 dosimetry
Karim Adinehvand (PhD)*1, Reza Yari (PhD)2 1. *Department of Radiotherapy, Borujerd Branch, Islamic Azad University, Borujerd, Iran. 2. Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Iran.
Introduction & Objectives: The most important cause of death in the world and Iran is coronary heart disease. Considering the importance of treating stroke in preventing myocardial infarction, nuclear medicine has suggested several methods, the most effective being coronary artery brachytherapy. Brachytherapy is a completely localized irradiation to a tumor tissue by one or more radioactive closed springs. It is important to calculate the dose received from cells and tissues to ensure unauthorized radiation is received. Therefore, the purpose of this study was to simulate the presence of electrons in adjacent tissues of the treated vessels. Materials & Methods: The MCNP or Monte-Carlo method was used to estimate the radiation dose distribution around the Iridium 192 radioactive source. The standard ORNL phantom and human vein were selected for this aim. The amount of dose received from the irradiation of a photon source with an average energy of about 0.38 MeV in the phantom and vessel simulation environment was investigated. Results: The findings indicate the significant presence of electrons due to the interaction of photons with matterand a high dose is absorbed outside the vascular tissue. Conclusion: According to the evaluations in the phantom environment for intravascular simulation, it was observed that the highest absorbed dose occurs outside the vein tissue. In evaluating absorbed dose, the importance of electrons in photon sources with biologically damaging effects should never be overlooked. Keywords: Brachytherapy, Iridium 192, MCNP method, Myocardial infarction
266 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 84414
An implantable smart electrospun nanofibers with switchable drug release for hyperthermic tumor cell treatment
Zahra Mohebian1, Mehdi Dadashpour2, Yones Pilehvar-Soltanahmadi3, Hanieh Mousazadeh4, Abbas Nobakht5, Nosratollah Zarghami6* 1. Department of Chemistry, Faculty of Science, Tabriz Branch- Islamic Azad University, Tabriz, Iran 2. Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran 3. Cellular and Molecular Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran 4. Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran 5. Research Institute for Fundamental Sciences (RIFS), University of Tabriz, Tabriz, Iran 6. Department of Clinical Biochemistry and Laboratory Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Abstarct: A smart polymeric electrospun nanofibers is described with concurrent heat generation and drug release in response to ‘on-off’ switching of alternating magnetic field (AMF) for induction of breast cancer apoptosis. The smart hyperthermia electrospun nanofibers are constructed via electrospinning temperature-responsive copolymer of NIPAAm and N- hydroxymethylacrylamide (HMAAm) (poly (NIPAAm-coHMAAm)) blended with magnetic nanoparticles (MNPs) and Cur. The prepared electrospun nanofibers characterized by Fourier transform infrared (FTIR) spectroscopy, 1H nuclear magnetic resonance (1H-NMR) spectroscopy and scanning electron microscopy (SEM). Correspondingly, the Cur loading efficiency and in vitro drug release of Cur from nanofibers were investigated by AMF and without magnetic field effect. The antitumor activity of synthesized nanofibers was also investigated on the breast cancer MCF7 cell lines. In vitro anti-tumor studies demonstrated that both MNP- and Cur-loaded nanofibers killed about 66% of cells, whereas only Cur-loaded nanofibers killed about 43% of cells. Therefore, an implantable smart magnetic nanofiber device can be used to both apply hyperthermia with AMF and to achieve cancer cell-specific drug release to enable synergistic cancer therapy. Taken together, our results not only provide an innovative smart magnetic nanofibers system as stimuli-responsive, synergistic chemo-thermal therapy platform for efficient reduction in the recurrence of breast cancer, but also achieve insight into the intricate interplay of the functional components in magnetic electrospun nanofibers. Keywords: Curcumin; Hyperthermia; Chemotherapy; Magnetic nanofiber
267
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 84521
Chronic myeloid leukemia and Klein Filters Syndrome
Negar Nouri1, Zahra Khalaj3, Maedeh Khosravi3, Seyyed Mehdi Kalantar2*, Fariborz Mokarian4,5, Vali Allah Mehrzad5, Mohammad Forat-Yazdi6, Fatemeh Shishebor7, Mansoor Salehi 3,8,9* 1. student research committee, Shahid Sadoughi university of Medical Sciences, Yazd, Iran. 2. Medical Genetic Research and Clinical Centre for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 3. Department of Genetics and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. 4. Cancer Prevention Research Center, Isfahan University of Medical Sciences, Isfahan, Iran. 5. Department of Medicine, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. 6. Department of Internal Medicine, Shahid Sadoughi Training Hospital, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran. 7. Pathology department, Shahid Sadoughi university of Medical Sciences, Yazd, Iran. 8. Cellular, Molecular and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran. 9. Medical Genetics Research Center of Genomw, Isfahan University of Medical Sciences, Isfahan, Iran.
Introduction: Chronic myeloid leukemia (CML) is a clonal abnormality of hematopoietic stem cells and the first human malignancy with an acquired genetic disorder known as Philadelphia chromosome (Ph). Symptoms of CML include high levels of white blood cell counts, splenomegaly, weight loss, lethargy and anemia. Recently, imatinib has been introduced as a conventional treatment for CML. Despite high efficacy of imatinib, some of patients show resistance. Here, imatinib dosage can be increased or other drugs may be prescribed. Klein Filters Syndrome is characterized as an endocrine disorder characterized by gynecomastia, hypogonadism, small testis and decreased follicle stimulating hormone (FSH) levels. The karyotype of patients is 47, XXY. The clinical features of infertility syndrome are delayed secondary sexual traits, gynecomastia, learning disabilities, decreased gonadotropin levels and low back hair. Description: Klein filter is associated with various hematologic malignancies such as AML, ALL, CML, CLL and lymphoma, however these malignancies are not common among patients. These patients are usually identified by chance during cytogenetic studies of neoplasms. Discussion and conclusion: Some oncogenes, such as those contribute to cell proliferation enhancement in PAR, should be considered in relation to Klein Filters syndrome and hematological disorders. Some studies have also suggested the association between Y chromosome abnormalities in Klein filter and CML and the formation of clones with abnormal chromosome t (9; 22). Healthy men and whom with Klein Filters, some others have reported no difference. Therefore, it is difficult to conclude Cases with acute, chronic leukemia and myelodysplasia concern about the increased susceptibility of chromosomal damage and formation of clones with abnormalities. A number of studies have been evaluated the severity and responsively of treatment to patients with Klein Filters syndrome who develop chronic myeloid
leukemia. Considering the contradictory results regarding the association between Klein Filters syndrome and chronic myeloid leukemia, further studies are needed.
268
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 84533
The Significance of GPC1+ circulating exosomes in early detection of Pancreatic Cancer
Moosa Rahimi1, Somaie kaveh Madavani2, Arezoo jokar1, Amir savardashtaki3 1. 1.. Laboratory of Common Basic Sciences, Mohammad Rasul Allah research center, Shiraz University of Medical Sciences, Shiraz, Iran 2. Laboratery of Zeinabieh hospital, Shiraz University of Medical Sciences, Shiraz, Iran 3. Department of Biotecnology, School of Advanced Medical Sciences and Technology, Shiraz University of Medical Sciences, Shiraz, Iran
Introduction: Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest types of cancer. The 5-year survival rate for PDAC is low because it is always diagnosed in late- stage and is resistant to treatment. Patients whose disease is diagnosed in its early stages have better outcomes. This is due to access to more treatment options, including surgery. Description: Early diagnosis of PDAC for better treatment is important. Liquid biopsy such as detecting the circulating tumor cells (CTCs), cell-free circulating nucleic acids (cfNAs), and microvesicles such as exosomes containing nucleic acids and proteins, which are released into the body fluid, usually into the blood, are methods that can diagnose cancer well. Bioinformatic analyses revealed 48 proteins in the cancer cell-derived exosomes. Glypican-1 (GPC1) is a membrane-anchored protein that overexpressed in a variety of cancers, including breast and pancreas cancer. Discussion and Conclusion: Analysis of liquid biopsy sample can be a good tool for early diagnosis, prognosis and for monitoring of responses to the primary tumor and metastatic disease, by taking multiple or serial biopsies, it allows us to monitor tumor facilitates characterization of Treatment allows selection of the optimal therapy according to changes in the therapeutic response. Several studies have shown that glypican-1 (GPC1), specifically enriched on cancer cell-derived exosomes is a good biomarker for early detection of pancreatic cancer. Today routine screening for PDAC in general population is MRI or CT that is very expensive and has a high false-positive rate. Several studies have shown that GPC1+ crExos is a reliable marker for the diagnosis of early PDAC. Keywords: Exosomes; Liquid biopsy; Pancreatic cancer; Glypican-1
269 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 84692
Circular RNAs (circRNAs) as novel diagnostic biomarkers for different diseases
Homa Mollaei*, Mahboubeh sadat Hosseinzadeh Department of Biology, Faculty of Sciences, University of Birjand, Birjand, Iran *Corresponding author E-mail: [email protected]
Introduction: Circular RNAs (circRNAs) are a novel class of endogenous noncoding RNAs and a field of much research interest and activity. In contrast linear RNAs, such as mRNAs, microRNAs (miRNAs) and long noncoding RNAs (lncRNAs), circRNAs are distinguished by a closed loop structure which is covalently formed by back-splicing process. Since 1970s, circRNA molecules were first discovered in RNA viruses, they have emerged as interesting biomolecules because of their high abundance, stability, specificity and conservation. Description: circRNAs have been reported to modulate gene expression post- transcriptionally by acting as miRNAs sponges or cooperating with RNA binding proteins (RBPs). Tissue-specific regulation of circRNAs expression has been found associated with initiation and progression of lots of diseases, including various kinds of cancers, cardiovascular diseases and neurological disorders. Up to now, circRNAs are candidate as biomarkers for different types of cancers, including colorectal, lung, breast, gastric, hepatocellular carcinoma liver and pancreatic cancers. Also, their potential utility as biomarkers for cardiovascular diseases, neurological disorders, diabetes, rheumatoid arthritis, pulmonary tuberculosis and different ocular diseases has been proposed. Discussion and conclusion: There are several advantages to employing circRNAs as disease biomarkers. Firstly, circRNAs structure with a covalently closed, continuous loop instead of polyadenylation tail makes them more resistant to exonucleases. Secondly, they can be easily detected in body fluid of patients as plasma, serum, saliva, urine and semen and they could serve as non-invasive biomarkers. Thirdly, as shown than circRNAs indicate stage-specific manner during cancer progression, compared to common carbohydrate antigens; their expression levels may be a better indicator of cancer dynamics. Despite recent advances in characterizing circRNAs in human diseases, their clinical application need to deeper understanding of molecular features. Developing bioinformatics and experimental methods including deep sequencing might lead to usage of circRNAs as novel powerful biomarkers in combination with current ones.
Keywords: circRNAs, biomarker, cancer
270
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 86261
Interaction between apolipoprotein B insertion/deletion polymorphisms and macronutrient intake with obesity risk, blood lipids, serum leptin and ghrelin in type 2 diabetes mellitus patients
Masoumeh Rafiee1*, Fariba Koohdani2, Reza Ghiasvand3 1- Department of Clinical Nutrition, School of Nutrition and food Sciences, Isfahan University of Medical Sciences, Isfahan, Iran 2- Department of cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran university of Medical Sciences, Tehran, Iran 3- Department of Community Nutrition, School of Nutrition and Food Science, Isfahan University of Medical Sciences, Isfahan, Iran
Objective: The aim of this study was to investigate the role of the association between the dietary intake of macronutrients with ApoB Insertion/Deletion variants on obesity risk, lipid profile, serum leptin and ghrelin in type 2 diabetes mellitus patients (T2DM). Methods: In this study, 700 T2DM patients were recruited. Anthropometric, biochemical and molecular data were collected, and Diet was assessed using FFQ. The interactions were tested using ANCOVA. Results: The carriers of the Del allele were associated with lower BMI and WC among individuals with high ω−3 intake (> 0.6% of energy), but not in those with low ω−3 intake (<0.6%). Also, when dietary ω−3 was <0.6%, general obesity risk in carriers of the Del allele was about 1.6 times higher than that of Ins/Ins homozygotes. But with high ω−3 intake (>0.6%), the risk was 0.5 times lower. Del-allele carriers with high MUFA and carbohydrate (≥ 12 and ≥ 54% of energy, respectively) had significantly higher TG and LDL-C compared to Ins/Ins, and these were not significant in subjects with low-MUFA and carbohydrate (< 12 and < 54%, respectively). Besides, Del-allele carriers with protein, SFA, MUFA and ω−3 of ≥ 14, 9, 12 and 0.6%, respectively, had a significant increase in their serum leptin than Ins/Ins homozygotes (P < 0.05). However, these associations were not significant between the two genetic groups in subjects with low intakes of protein, SFA, MUFA and ω−3. Moreover, Del-allele carriers with low carbohydrate (< 54%) had significantly higher leptin and ghrelin than Ins/Ins homozygotes (P < 0.05), however, in high- carbohydrate group, leptin and ghrelin were not significantly lower.
Conclusions: Apo B Ins/Del SNPs showed potential interaction with MUFA, SFA, ω−3. carbohydrate and protein to modulate serum levels of TG, LDL-C, leptin, ghrelin and obesity risk in T2DM patients.
271 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 86852
A review of Electrospun Nanofiber Poly L-Lactic Acid scaffold application for angiogenesis in biomedical researches
Sara Anajafi1*, Pegah Rahimi Zadeh2 1. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 2. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive
Introduction: Tissue engineering is one of the most practical fields of regenerative medicine that integrates engineering and biology sciences to substitute, restore, and cure injured tissues. However, tissue engineering is not yet a true clinical reality due to some complications. One of the main problems is insufficient vascularization among the construct. Scaffolds which are artificial extracellular matrices play a crucial role in the survival of tissues. Description: Electrospun Poly l-lactic acid (PLLA) scaffolds made of interconnected pores with suitable mechanical attributes can be manufactured in sheet or tubular form; also it would be easily coated by other compounds like hydroxyapatite and many other inductive agents. PLLA shows high capacity in terms of composition with vast range of materials. These features made it an interesting scaffold for many tissue engineering types of research. Discussion and conclusion: The exploration of an efficient strategy to significantly improve angiogenesis besides practical features of PLLA leads scientists to conduct many investigations. This review will provide an overview of PLLA roles and effects in angiogenesis researches, like bone tissue engineering and diabetic wound investigations. Keywords: Angiogenesis, Poly l-lactic acid, Tissue engineering, Scaffold.
272 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 87056
Bioinformatics study of fatty acid ester in fungus
Farzane Kargar, Mojtaba Mortazavia*, Masoud Torkzadeh Mahania, Amir Savardashtaki, Rana Jahanban, Pegah Hejazi a. Department of Biotechnology, Institute of Science and High Technology and Environmental Science, Graduate University of Advanced Technology, Kerman, Iran b. Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies Shiraz University of Medical Sciences Shiraz Iran c. Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies Tabriz University of Medical Sciences Tabriz Iran
Abstract: A fungus is any member of the group of eukaryotic organisms that includes microorganisms such as yeasts and molds, as well as the more familiar mushrooms. The fungal fatty acids have the critical properties as the antioxidant and anti-mutagenic activity that are very important. In this regard, the previous studies have led to the identification of the new fatty acids in the Rhizopogon luteolus fungus which was discovered in the early twentieth century. These fatty acids that have been beneficial for human. Phytochemical investigation of the Rhizopogon luteolus led to the isolation of one new fatty acid ester, 3- hydroxy-2,4-dimethylheptacosyl acetate together with two known compounds. The 2D structure of this fatty acid was saved and evaluated as pdb file. This three-dimensional structure was optimized using the Gaussian program with a base set of 6-31g * and b3lyp computational layers. The final model was introduced in the Marvin Sketch software for others properties. The similarity and proximity of the sexes was drawn which represents the low difference between the amino acid sequences. This fatty acid contains 31 carbon atoms and 62 hydrogen atoms and 3 oxygen atoms. As the result show, no isoelectric point was identified in this structure. Keywords: Rhizopogon luteolus, hepta-cocyl acetate, Phylogenetic tree, Bioinformatics
273 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 87296
Annexin A1: as novel biomarkers for screening, diagnosis, and monitoring of female reproductive system cancer
Houshang Nemati1, Mehri Nazeri2
1- Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran 2- Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
Abstract: Female reproductive system cancer is among the most common malignancies and the primary causes of cancer-related death in women worldwide. The clinical outcome of patients has a close relationship to early and accurate diagnosis of reproductive system carcinoma. Annexin A1 (ANXA1) known as an anti-inflammatory factor which plays a critical role in the proliferation, apoptosis, differentiation, angiogenesis, and metastasis. Previous studies suggested ANXA1 N-terminal peptide Ac2-26 as an anti- proliferation agent in cervical cancer cells by activating the MAPK cascade and targeting p53. Little is known about the clinical significance and diagnosis potential of Annexin A1 as biomarker to diagnosis of the female reproductive system cancer. The present paper aims to provide an overview of the research surrounding the ANXA1 potential as a therapeutic and diagnostic factor and its mechanism of action in female reproductive system cancer. Keywords : Female, Biomarkers, Annexin A1, reproductive system cancer, Peptides
274 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 88075
Application of 3D Bio-Printing in Regenerative medicine and Tissue Engineering
Mehdi Atari1, Sheyda Labbaf2, Shaghayegh Haghjooy Javanmard3 1,2. Biomatrials Research Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran 3. Department of Physiology, Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
Introduction: 3D printing technology is now recognized as a novel and advanced approach in regenerative medicine as it is capable of overcoming the limitations associated with other conventional processing methods. This technology enables the creation of a structure with cells, synthetic and natural biomaterials, growth factors and biomolecules in a 3D construct with the aim of mimicking natural tissue structure. Description: In tissue engineering, the use of advanced tools and technology with the ability to fabricate and manage layer by layer structures can be an effective approach in the success of tissue reconstruction and repair of the defected site. 3D printing technology, utilizing cells embedded in a hydrogel, are able to create an extracellular matrix structure similar to the host tissue. They are various parameters that contribute to printer’s biological ink (Bio-ink) such as type and concentration of the polymer and the solvent. All of the effective parameters in 3D bio-printing is thoroughly discussed. Discussion and Conclusion: One of the most recent advances in biomedicine is the application of 3D biomaterials processing method which can enable the creation of a patient-specific construct. At present, although the clinical applications of 3D printer face limitations and challenges, but such structures are capable of angiogenesis and differentiation and the direct growth in the near future. Keyword: 3D Bio printer, Regenerative medicine, Hydrogel, Scaffold.
275 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 88239
Co-Culture of mesenchymal stem cells with endothelial progenitor cells inside alginate-gelatin microspheres promoted angiogenic potential
Shirin Saberianpour1*, Reza Rahbarghazi1, Mahdi Ahmadi1, Mohammad Nouri1, Morteza heidarzadeh1, Abbas Karimi1, Souror Nemati1 1- Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Introduction: Here, we investigated the angiogenic potential of endothelial progenitor cells juxtaposed with mesenchymal stem cells inside alginate-gelatin microspheres . Methods: Three encapsulated groups were allocated including EPCs, MSCs, EPCs+MSCs. Cells were encapsulated with a mixture of 1% alginate and 2% gelatin hydrogel. Cell survival was examined by MTT assay. Endothelial differentiation was determined by flow cytometry and ELISA. Tubulogenesis assay and Ac-Dil-LDL uptake were used to detect functional activity. Cell migration was analyzed by Transwell insert and gelatin zymography analyses. By using real-time PCR, we measured the transcription of Akt and PK1. Results: We found an increase in cell viability in MSCs/SDF-1α microspheres compared to EPCs group (p<0.05). EPC/MSCs co-culture contributed to the increase of CD133+ cells while we found high CD31 levels in MSCs group (p<0.05). Juxtaposition of EPC with MSCs increased tubulogenesis compared to Culture of cells individually . Conclusions: Alginate-gelatin microspheres could alter the angiogenic potential of progenitor cells . Keywords: Endothelial Progenitor Cells; Mesenchymal Stem Cells; Alginate-gelatin Microspheres; Angiogenesis
276 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 88491
Application of Various Stem Cells for Nerve Tissue Engineering
Maliheh Jahromi, Shahnaz Razavi, Reihaneh Seyedebrahimi Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract: Peripheral nerve injury has remained a clinical hurdle despite its frequency and advances in treatment. Cell‐based therapy in peripheral nerve injuries has been interested by researchers in the field of nerve regenerative medicine. Cell transplantation by nerve conduits can be used effectively to promote axonal regeneration. Some factors in determining successful outcomes of cell therapy on nerve regeneration are production of ECM proteins and trophic factors creating a favorable environment for axonal outgrowth, stimulation of angiogenesis, differentiation into Schwann cell (SC) lineage, facilitation of myelination of regenerating axons, immunosupression in the damaged nerve, and stimulation of differentiation and proliferation of endogenous cells. Numerous cells have been applied inside nerve conduits such as SCs and different types of stem cells. In peripheral nerve regeneration, the most extensively applied stem cells are embryonic stem cells, dental pulp stem cells (DPSCs), adipose stem cells, bone marrow MSCs (BMMSCs), neural crest stem cells, and induced pluripotent stem cells (iPSCs). The purpose of this review is current points of view on the application of stem cell based strategy for peripheral nerve regeneration.
277 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 88717
The relationship between hot-wet temperament in Iranian Traditional Medicine (ITM) and Non-alcoholic Fatty Liver Disease (NAFLD) from systems biology point of view
Seyedeh Sadaf Behbahani Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran; Email: [email protected], Postal address: Science and Research Branch, Daneshgah Blvd, Simon Bulivar Blvd, Tehran
Introduction and Objectives: Iranian Traditional Medicine (ITM) has a holistic approach on disease and treatment and has always had a lack of molecular biology evidence; ITM has a common approach with systems biology which considers the life as a system consisting the components and their interactions. The aim of this study is to stablish a connection between ITM and conventional medicine through expressing ITM in the form of modern molecular biology. Materials and Methods: In this study, GEO2R software was used to find the genes for Non-alcoholic Fatty Liver Disease (NAFLD) and the genes for hot-wet temperament was obtained by a previous study using proteomics. Then the genes of NAFLD were compared with the genes of hot-wet temperament to find the potential common genes between them and the pathways related to these common genes were extracted. Results: Based on this comparison, three genes were common between NAFLD and hot- wet temperament. It was found that the pathways associated with these common genes are important in NAFLD and its progression. Conclusion: These results could explain why people with hot-wet temperament develop NAFLD.
Keywords Iranian Traditional Medicine (ITM), Systems biology, Non-Alcoholic Fatty Liver Disease (NAFLD), Temperament
278
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 88744
Bioinformatics evaluation of targetom has-miR-128-3p signaling pathways and related function of ALDH2, SNP (rs671) in patients withacute lymphoblastic leukemia
Negar Bahadorzehi1, Massoud Houshmand2, Nasrin Fattahi3, Atefeh Zamani3
1. Cellular-Molecular Division, Department of Biology, High Institute Nurdanesh, Meymeh, Isfahan, Iran 2. Medical Genetic Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran 3. Gene Raz Buali, Genetic and Biotechnology Academy, Isfahan, Iran
Abstract:
Hematologic malignancies, account for a significant percentage of cancers in worldwide. One of the most common types of malignancies, is acute lymphoblastic leukemia (ALL). ALL is One of the major blood cancers in humans. Different genetics and environment risk factors can cause this cancer. Therefor for finding more bioinformatic information we used NCBI,miRbase, miRWALK2.0, Target scan, DAVID database and KEGG pathway.ALDH2 is the second enzyme of the major oxidative pathway of alcohol metabolism. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. Expression of this gene is reduced in leukemia and bone marrow failure, leading to worsening leukemia.has-miR-128-3p is expected to target the ALDH2 gene in the pathway of cell proliferation that leads to leukemia. Because of the position of rs671 in the coding region of the ALDH2 gene, this microRNA is the precisely the part of the gene. It is likely to target this polymorphism.In the previous studyof has-miR-128-3p in people with acute lymphoblastic leukemia, they found that this microRNA is overexpressed and effective as an oncomiR in the development and progression of ALL.In this study, if bioinformatics predicted that the binding site of this microRNA is exactly the same as the rs671 allele and the risk allele is the mutant allele, and the expected expression of ALDH2 and the negative regulatory function of the microRNAs would be expected. has-miR-128-3p increase and then decrease the expression of its target gene. For this reason, the has-miR-128-3p is predicted to have a higher binding capacity to the rs671 mutant (A) allele than to the dominant (G) allele of this SNP and may therefore be a risky mutant allele and cause cancer.
Keyword: Acute lymphoblastic leukemia,Single nucleotide polymorphism, rs671, has-miR- 128-3p,ALDH2
279 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 88914
The Effect of Dracocephalum Kotschyi Alcoholic Extract on the BAX Expression in SKBR3 Cell Line
Afrooz Golestanian1,2, Farinaz Khosravian1,2,3, Nasrin Hadi4, Fatemeh ketabchi3, Mirza Mohammad Raisinia5, Mansoor Salehi1,2,3 1. Cellular, Molecular and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran. 2. Medical Genetics Research Center of Genome, Isfahan, Iran. 3. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. 4. Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran. 5. Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran.
Introduction and Objectives: Breast cancer is considered as the most common disease threatening women around the world which is the fifth cause of death in Iran. Over the past few decades, derived compounds from plants have been a major source of anticancer clinical substances. Therefore, the effect of Dracocephalum Katschyi alcoholic extract on proliferation and BCL2 genes expression level on SKBR3 cell line in breast cancer is investigated in this study. Dracocephalum Kotschyi consists of apprximately one hundred different compounds such as Terpenoids, Flavonoids, and Alkaloids which are antioxidants. To achieve our goals different samples were prepared and were tested. Materials and Methods: One hundred grams of powder was produced from the Dracocephalum leaves and aerial parts were solved in 250 ml ethanol. The mix ratios were chosen to obtain samples having 10, 50, 100, and 500µg/ml concentrations. In addition, SKBR3 cell lines were cultured under 5% CO2 at 37 °C in DMEM. The effects of an alcoholic agent on cell line as well as BCL2 expression was investigated through exposing them for 24 hours. cDNA was synthesized from the extracted RNAs by a QuantiTed Reverse TranscriptionKit. Next, the BCL2 expression was evaluated. Results: An opposite correlation between the concentration of Dracocephalum Kotschyi and the cell proliferation suggested the anticancer characteristic of this plant. SKBR3 cells IC50 value in 24hrs was 102.1 and in 500µg/ml the most cell viability decrease was observed. Conclusions: The following conclusion can be drawn from the results of this investigation. Since Dracocephalum Katschyi alcoholic extract results in BCL2 expression decrease, it can be used in producing new anticancer medicines. Keywords: BCL2, Breast Cancer, Dracocephalum Kotschyi
280
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 89346
In silico Analysis of caffeine effects on NMDA receptor in Alzheimer’s disease
Fazel Mohammadi1, Fariba Dehghanian1 1. Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.
Introduction: NMDAR is a glutamate receptor on the surface of neurons that acts as an ionic receptor. In the second phase of Alzheimer's disease (AD), the oligomer peptide of Beta-amyloid activates the downstream signaling pathway by binding to this receptor which leads to cell apoptosis. Studies have shown that inhibiting this receptor in AD patients can prevent the destruction of nerve cells. Caffeine in coffee and its effect on Alzheimer's disease has been known in the early stages of AD. We investigated the inhibitory effect of caffeine and its primary metabolites on NMDAR through computer simulated docking analysis. Materials and methods: Molecular docking is one of the structure-based computational methods used in drug design. In this study, we selected the structure of the NMDAR using PDB database. Caffeine, Paraxanthine, Theophylline, Theobromine, and a known NMDAR inhibitor called Memantine were used. The molecular docking process was performed using Molegro Virtual Docker V6.0 software. The results of docking based on bond free energy (Delta G) are investigated. Results: The comparative activity of caffeine, its primary metabolites and Memantine were analyzed by docking score and binding energy. The MolDock score of Caffeine, Paraxanthine, Theophylline, Theobromine and Memantine is -71.7622, -67.6633, -74.667, -72.6754 and -69.7672 kcal/mol respectively. Conclusion: Our results indicate the lower free binding energy and stronger binding affinity of caffeine and its primary metabolites to NMDAR compared to Memantine as a standard drug for AD. Therefore, it can be suggested that Caffeine has better inhibitory effects on NMDAR. In conclusion, this study shows the importance of natural small molecule screening and their use for an efficient drug discovery process. Keywords: Alzheimer's disease, NMDAR, Molecular docking, Molegro, Memantine, Caffeine
281 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 90666
A Bioinformatics approach to identify overlapped genes between SARS-COV- 2 and lung cancer
Faeze Ahmadi1,2, Mohammad Kazemi1, Reza Mousavi2 1. Genetics and Molecular Biology department, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 2. Cellular, Molecular and Genetic Research Center of Genome, Isfahan University of Medical Sciences, Isfahan, Iran
Introduction and Objectives: Today, Covid-19 is the most important agent of death and respiratory illnesses all around the world. Lung cancer is a disease with symptoms of SARS-CoV-2, and discovery of relationship between two diseases can be useful. This study aims to illuminate the overlapped genes between SARS-CoV-2 and lung cancer. Materials and method: A gene expression profile (GSE-1739) was obtained from GEO database. Analysis of dataset performed by GEO2R and differentially expressed genes (DEGs) with adjusted p value less than 0.05 were identified. A gene expression profile (GSE-21933) was reviewed and its analysis performed similar to SARS dataset. In the next step “venn diagram tool” used to obtain overlapped genes between SARS and lung cancer. Then, STRING database was used to construct a PPI network of overlapped genes (minimum required interaction score >0.7). The genes with the most interactions were selected and transferred to “g:profiler database” and “reactome.org” to determine their functional profiling. Results: 27 genes (ANXA3, ELANE, BIRC5, HP, PPID, CASP8AP2, NR3C2, PRKCH, STAM2, LIN7C, CEBPE, GINS2, TIA1, DEFA4, CCT2, CYP4F3, SLPI, CAMP, ITGAM, TCN1, KIF20A, TXN, CASP2, CENPF, HLX, BIRC3, MMP9) were found that were common between SARS and lung cancer. The 9 genes (ITGAM, MMP9, TCN1, CAMP, DEFA4, SLPI, HP, ELANE, CCT2) interact strongly with each other. These are the genes that have significant roles in immune system processes. Conclusion: The findings support the prediction that the most important genes in SARS- COV-2 and lung cancer have significant overlap to each other and have important rolls in immune system biological processes. SO, perhaps it can be concluded that the underlying mechanisms of SARS-COV-2 is similar to lung cancer and it may be possible to use common drugs and treats of lung cancer for reducing symptoms and unpleasant effects of SARS-COV-2. Keywords: SARS-COV-2, Lung cancer, Overlapped genes, Bioinformatics analysis
282
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 91203
An in silico approach to design a novel multi-epitope peptide vaccine against Helicobacter Pylori
Elham Jafari ª, Shirin Mahmoodi ª ª Department of Medical Biotechnology, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran
Introduction & Objectives: Helicobacter pylori, the most common human infectious agent, is a gram-negative bacterium that colonizes in the stomach and causes gastritis, peptic ulcers, adenocarcinoma, and MALT (mucosa-associated lymphoid tissue) lymphoma. This bacterium which has recognized as carcinogen by the World Health Organization (WHO), has infected 50% of the world population on average. Although because of its high prevalence rate and antibiotics resistance, development of a vaccine has been accepted as an efficient strategy in prevention of mentioned cancers, it has remained as a challenge so far and today immunoinformatics is a promising way that helps to solve this problem. Methods: In this study, three H. Pylori antigens, including UreB, HpaA, and NapA were selected which have an important role in its pathogenicity or colonization. Since the T CD4 cells are the most important of the immune system cells against H. Pylori, so their potential immunodominant T CD4 epitopes were selected by four relevant servers ⁺ including IEDB, MHC2Pred, MHCPred, and RANKPEP and after evaluation by IFNepitope server, were joined to each⁺ other by KK and GPGPG linkers. As adjuvant, flagellin D0 and D1 domains were added to each side of the construct by a flexible linker, EAAAK, to increase its immunity. Then the designed vaccine was evaluated in multiple aspects. Results: The final construct was estimated as antigenic (0.93) and nonallergenic with appropriate solubility (0.63), molecular weight (64581.40), isoelectric pH (9.37), and instability index (29.32). Conclusion: Based on the current study, this vaccine can serve as a promising anti-H. Pylori vaccine and gastric cancer-preventive candidate. Keywords: Helicobacter Pylori, Vaccine, UreB, Gastric cancer, Immunoinformatics, Design
283 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 91837
Co-transplantation of human neurotrophic factors secreting cells and adipose- derived stem cells in rat model of MS
Shahnaz Razavi1*, Nazem Ghasemi1 Department of Anatomical Science, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Objective: The transdifferentiation of human adipose-derived stem cells (ADSCs) into Schwann-like cells on biocomposite scaffolds may be a critical issue in nerve regeneration medicine. In this study, tissue-engineered scaffold with chitosan nano powders (CS) and poly (lactide-co-glycolide) (PLGA) was investigated for its potential Schwann cells (SCs) transdifferentiation. Materials and Methods: The differentiation of human ADSCs into S-like cells was induced with different CS content and direction of nanofibers on PLGA/CS scaffolds. Cell morphology and proliferation of differentiated cells were investigated by scanning electron microscopy and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay respectively. For assessment efficiency of transdifferentiation, the expression of SC markers (glial fibrillary acidic protein and S100), and myelinogenic marker (myelin basic protein) were investigated in different nanochitosan content and direction of nanofibers scaffolds, using immunocytochemistry technique. Results: The nanochitosan can significantly promote cell proliferation of differentiated cells (p< 0.05). The mean percentage of S-like cells on greater CS content nanofibers scaffold was significantly higher than others (p< 0.05). In addition, the align orientation of nanofibers in scaffolds guided the differentiation of ADSCs toward myelinating S-like cells on the constructs. Conclusion: Overall, we found that high CS content and aligned-orientation of nanofibers in biocomposite scaffold (70/30A) can promote differentiation and myelinogenic capacity of S-like cells induced from human ADSCs. Keywords: ADSCs, PLGA/CS, Nanocamposite, transdifferentiation, Schwann-like cell.
284 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 91963
The role of oral magnesium sulfate in the stimulation of PPAR-γ and inhibition of NFKB genes expression in improving hyperglycemia in the muscle of STZ-induced diabetic rat
Fatemeh Khosravi1, Fatemeh Kharazmi1, Mitra Kamran1, Nepton Soltani2* 1. Molecular Medicine Research Center and Hormozgan Health Institute, Hormozgan University of Medical Science, Bandar Abbas, Iran 2. Endocrinology and Metabolism Research Center, Hormozgan University of Medical Science, Bandar Abbas, Iran 3. Physiology Department, Faculty of medicine, Hormozgan University of Medical Science, Bandar Abbas, Iran 4. Clinical pathology Department, Faculty of medicine, Isfahan University of Medical Science, Isfahan, Iran
Introduction: The aim of this study was to investigate the possible role of magnesium (Mg2+) on activation of the peroxisome proliferator-activated receptor gamma (PPAR-γ) and inhibition of nuclear factor-KB (NFKB p65) in muscle to increase glucose transporter 4 (GLUT4) gene expression. Methods. Fifty rats were divided into five groups, namely non-diabetic control (NDC), Mg2+-treated non-diabetic control (Mg2+-NDC), chronic diabetic (CD), Mg2+-treated chronic diabetic (Mg2+-CD), and insulin-treated chronic diabetic (Ins-CD). Diabetes was induced with streptozotocin (STZ) injection. The Mg2+-CD and Mg2+-NDC groups
received 10g/l of magnesium sulfate (MgSOR4R) added to drinking water and Ins-CD group received 2.5 U/kg of insulin. The fasting blood glucose level and body weight were measured every week. After 16 weeks, intraperitoneal glucose tolerance test (IPGTT) was done and then animals were decapitated, blood samples were taken to determine the plasma levels of Mg2+ and gastrocnemius muscle legs were isolated for both PPAR-γ and NFKB (p65) genes and proteins expression. Results. Administration of MgSO4 improved IPGTT, lowered blood glucose levels and increased PPAR-γ gene and protein expression. Diabetes increased NFKB gene and protein expression. Although Mg2+ therapy could not decrease NFKB (p65) gene expression, the protein decreased by Mg2+ therapy. Insulin decreased NFKB (p65) gene and protein expression, without any effect on PPAR-γ gene and protein expression. Conclusion. Findings suggested that Mg2+ decreases blood glucose levels via suppressing NFKB (p65) protein synthesis and increases PPAR-γ gene and protein expression. But insulin could decrease blood glucose level through a decrease in NFKB (p65) gene and protein expression. Keywords: Diabetes, Insulin, Magnesium, PPAR-γ, NFKB (p65)
285
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 93017
Oral mucosal mesenchymal stem cells
Saba Bagheri1*, Farzane Jabari2, Reza Salarian3 1- Department of Biomedical Engineering, Maziar Higher Education Institute, Royan, Iran 2- Ph.D of Biornedical Engineering Nanotechnology and Advanced Materials Department, Materials and Energy Research Center (Merc), Alborz, Iran 3- Ph.D of Biomedical Engineering, Maziar Higher Education Institute, Royan, Iran
Introduction: Oral mucosal tissue is developmentally surrounded by the jawbone and masseter tissues from the neural crest-origin pharyngeal arches, and it also originates from the embryonic neural crest. Several recent studies have reported that oral mucosa-derived stem cells contain neural crest-origin cells that potentially differentiate into neurons. Description: In the context of this overview, 16 articles were used between the years 2010 through the end of 2018 using the Keywords of PubMed, Google scholar search engines. Discussion and conclusion: Many studies reported that neural crest and neural markers were expressed in hOMFs, which might contain both MSCs and neural crest-origin cells. Researchers reported that hOMFs are a more suitable alternative to 3T3 feeder cells than human limbal fibroblasts in the production of epithelial sheets. For clinical use, we are required to supply stable and well-characterized epithelial sheets and information on the preparation of the feeder cells. Feeder cells exhibit different characteristics based on their tissue of isolation, harvest site and the individual donors. Factors important for ocular surface reconstruction include low-affinity neurotrophin receptor p75, an early neural crest-related factor and receptor of NGF, brain-derived neurotropic factor and neurotrophin-3/4/5, expressed in human corneal epithelium, oral mucosa epithelium, skin and neurons as regulatory factors that mediate cell survival, differentiation, proliferation and plasticity. Recent studies have reported that the limbal stem/progenitor cells were preserved by culture conditions combining EGF and NGF, and human limbal epithelial cells on amniotic membranes were expanded through NGF signaling. In many studies, researchers isolated and cultured hOMFs containing neural crest-origin cells, and demonstrated their ability to differentiate into both mesenchymal and neural cell lineages, including corneal keratocytes, and their utility as a substitute for xenobiotic 3T3 feeder cells in corneal or oral mucosal-stratified epithelial cell sheets. By identifying the characteristics and abilities of hOMFs containing neural crest-derived cells, we anticipate their use as a new autologous cell source in refractory diseases of neural crest-derived tissues such as ocular surfaces.
Keywords: Mesenchymal,Stemcells, Oral Mucosal
286
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 93131
Resveratrol and Gliomas: Where are we standing?
Maryam Fallah1, Hamed Mirzaei2 1. Student Research Committee, Kashan University of Medical Sciences, Kashan, I.R. Iran 2. Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, I.R. Iran
Abstract: Gliomas are well-known brain and spinal cord tumors. These tumors are a heterogeneous group of brain tumors with different biological and clinical features. Surgery and chemotherapy are known as current therapeutic platform in the treatment of various types of gliomas. Despite different advances in the therapeutic approaches in the treatment of gliomas, several limitations are associated with them. Hence, finding and developing new therapeutic option are necessary. Plans medicine are new horizons which could be employed as new anti-cancer agents. Resveratrol is well known natural compounds that can be used as therapeutic agent alone or in combination with other chemotherapy drugs. Resveratrol shows pharmacology activities such as anti-cancer, anti-aging, anti- inflammatory, chemotherapeutic, cardio protective, and neuroprotective action. Several studies indicated that resveratrol exerts therapeutic effects on glioma cells. Given that this natural compound exerts its therapeutic impacts on gliomas via targeting different physiological and pathological events such as apoptosis, growth, angiogenesis, and metastasis. Herein, we summarized the anti-gliomas effects of resveratrol.
287 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 93216
The Impact of Environmental Contaminants on Telomere Length
Bahare Dehdashti1, Roya Kelishadi2, Mohammad Mehdi Amin3*
1- Student Research Committee and Department of Environmental Health Engineering, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran. 2- Child Growth and Development Research center andResearch Institute for Primordial Prevention of Non-communicable disease, Isfahan University of Medical Sciences, Isfahan, Iran. 3- Environment Research Center, Research Institute for Primordial Prevention of Non-communicable disease, and Department of Environmental Health Engineering, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran.
Introduction & Objective: Nowadays, numerous studies have been conducted to evaluate the different effects of environmental pollutants on fetal intrauterine conditions at birth and the results indicate that these factors have a negative effect. Heavy metals including arsenic, lead, cadmium, selenium etc. are distributed in the environment due to anthropogenic activities such as mining, coal burning, industrial gas emissions, household waste and the use and disposal of products. Polycyclic aromatic hydrocarbons (PAHs) caused by incomplete combustion of fossilsfuels, tobacco and other organic matters. Polychlorinated biphenyls (PCBs), and phthalates are used in plastics, solvents or goods such as cosmetics, toys, and food packaging and construction materials.Organophosphorus pesticides are used in agriculture, home and garden. These pollutants are produced in industry, commerce agriculture, municipal and household activities. Most of these pollutants have the ability to cross the placental barrier and thus have effects on the fetus. Therefore, exposure to them during pregnancy may eventually affect the baby. Telomeres are duplicate DNA sequences (TTAGGGs) located at the ends of eukaryotic chromosomes that, after each cell division, are shorter in normal somatic cells to prevent chromosomal fusion. When the telomere reaches a certain length, cell division stops and cell apoptosis occurs. Changes in telomere length among individuals largely depend on natural changes at birth, so early telomere length is important throughout life. It is considered as a marker of cellular aging and is associated with an increased risk of age-related diseases including cardiovascular disease as well as mortality. Because telomeres are highly sensitive to oxidative stress, exposure to environmental contaminants may be directly affected by oxidative stress.The purpose of this study is to review the effects of environmental pollutants on telomere length reduction. Materials & Methods: The search process was performed on valid electronic citation databases including Google Scholar, Scopus, PubMed and Web of Science using the keywords Telomere length, Environmental Contaminants, pregnancy, pregnancy outcome. Results: Results showed that exposure to phthalate metabolites in the first trimester of pregnancy was inversely related to telomere length. No difference observedbetween these metabolites in the first trimester of pregnancy and telomere length of cord blood in both sexes. Also, in relation to polycyclic aromatic hydrocarbons, there was an inverse relationship between PAHs metabolites and telomere length and neurobehavioral growth in children, with a decrease in telomere length and growth as metabolites increased. In another study on the effects of cadmium and a brief review of leukocyte telomere length in newborn infants in China, the results also showed that maternal exposure to cadmium decreased cord blood telomere length. Long and short-term exposure to PM2.5 and polychlorinated biphenyls, indicated an inverse relationship between telomere length and long-term exposure and a direct relationship to short-term exposure to contaminants and telomere length, respectively. Perfluoroalkyl and polyfluoroalkyl also as stable organic matters and constituents crossing the maternal and fetal barrier during pregnancy had an effect on telomere length, which resulted in a decrease in telomere length due to the reaction of these contaminants and the effects of neonatal sex. Reactive chemicals containing oxygen (ROS), increased oxidative stress. Conclusions: Given the adverse effects of environmental pollutants on the health of pregnant mothers and newborns as a vulnerable and vulnerable group of society, and the inadequacy of studies on some diseases and abnormalities, researchers are advised to pay more attention to this issue. They are focused. managers also plan and adopt health policies and applications to prevent the development of environmental pollutants and implement effective remedies.
Key Words: Telomere Shortening, Environmental Contaminants, Heavy Metals, phthalates
288 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 94358
Effects of Rheum ribes on paraoxonase1 activity in hyperlipidemia rats
Mahdi Rafiee1, Javad Saffari-Chaleshtori2, Gholamreza Mobini3 1. Student Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran 2. Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran 3. Medical plants, Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
Introduction & objectives: Paraoxonese1 (PON1) is an enzyme associated with high density lipoprotein (HDL) in serum, with aryl esterase and paraoxonase activity. The antioxidant properties of this enzyme protect the oxidation of low density lipoprotein (LDL). Certain antioxidant compounds and medical plants, such as Rheum ribes, can protect the LDL from oxidation. In this study, we evaluated the effects of Rheum ribes on the PON1 enzyme in hyperlipidemic and hypercholesterolemia rats and investigated the molecular dynamic effects of Cinamic , Glucogallin and Quercetin (the most compounds of Rheum ribes) On PON1 activity. Materials & Methods: We used hydro-alcoholic extract of Rheum ribes in hyperlipidemia and hypercholesterolemia rats for the demonstration of the PON1 enzyme activity by aryl esterase properties and the PON1 gene expression. Then, we demonstrated the molecular dynamic (MD) effects of their compounds (Cinamic , Glucogallin and Quercetin) on PON1 via simulation studies using the AutoDockv:4.0.0 and the Gromacs 2018 software. Results: Rheum ribes reduced the serum triglycerides (TG) and total cholesterol (TC), and increased the PON1 serum aryl esterase activity, however, had no significant effect on the PON1 gene expression. The simulation and the MD results proved that among all the compounds, Glucogallin and Quercetin have more tendencies to bind to the enzyme. However, the radius of gyration (RG), increased when three compounds bind to PON1. Conclusion: Rheum ribes can increase the PON1 activity in serum, without increasing the gene expression. According to simulation and MD studies, Glucogallin and Quercetin approximately had the most important for increasing the PON1 activity. Keywords: Rheum ribes; PON1 enzyme; Molecular dynam
289 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 95116
Role of trehalose on structural and functional stability of D-lactate dehydrogenase enzyme
Faranak Saeidnia1*, Maryam Zaboli2, Masoud Torkzadeh-Mahani2 1- Department of Biotechnology, Institute of Science, High Technology & Environmental Science, Graduate University of Advanced Technology, Kerman, Iran 2- Department of chemistry, faculty of science, University of Birjand, Birjand, Iran
Introduction & Objectives: Lactate dehydrogenase (LDH) belongs to the family of oxidoreductases and catalyzes the reduction of NADH and pyruvate to NAD+ and D- lactate in the final step of anaerobic glycolysis. D-Lactate dehydrogenase (D-LDH) is a marker of common injuries and diseases such as kidney stones, heart failure, some types of cancers and appendicitis. Accordingly, biosensor design and construction is very significant to measure blood lactate levels. The thermal sensitivity of D-LDH is limiting the usage of this protein in certain clinical and diagnostic applications. Trehalose has been known to be a superior stabilizer in providing protection to biological materials against dehydration and desiccation. In this work, trehalose was examined as a support for increasing the thermal stability of D-LDH. Materials & Methods: In this work, the effect of different concentrations of trehalose on the stabilization of recombinant D-LDH was studied. The Specific activity of free D-LDH and D-LDH/trehalose was calculated using the reduction of absorbance in 340 nm due to the oxidation of NADH. The kinetic and thermodynamic parameters, optimum temperature and pH, and the intrinsic fluorescence of free and treated enzymes were examined. Also, an attempt was made to investigate the effect of trehalose on the conformation of UOX in the atomistic level using molecular dynamics (MD) simulations. Results: Additives like trehalose protect the D-LDH conformation, increases its activity and also, improves its stability at high temperatures. Trehalose is a suitable additive for stabilization of the D-LDH. The activity of the enzyme was increased in the presence of trehalose. Also, the RMSD, RMSF and secondary structure analysis indicated that the trehalose protects the enzyme conformation. Conclusion: Results show that trehalose protects the D-LDH conformation and improves enzyme stability at high temperatures. Therefore, trehalose is a suitable additive for stabilization of the D-LDH.
Keywords: D-Lactate dehydrogenase, stabilization, trehalose, molecular dynamics simulation
290 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 95560
Introducing New Inhibitors for Kallikrein-related peptidase 6 (KLK6)
Fatemeh Mahmoodi1*, Hamid Bakherad2, Navid Mogharab1, Mohammad Rabbani2 1. Department of Cell and Molecullar Biology, College of Sciences, Shiraz University, Shiraz, Iran. 2. Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Introduction and Objectives: The human Kallikrein-related peptidase 6 (KLK6) gene belongs to the 15-member Kallikrein gene family mapping to chromosome 19q13.3. Encoding for an enzyme with trypsin-like properties. The observed highly upregulated expression in various cancers, neurodegenerative diseases and skin conditions has led to the discovery that KLK6 participates in other cellular pathways including inflammation, receptor activation and regulation of apoptosis. KLK6 can degrade components of the extracellular matrix and increased activity promotes cancer invasion and metastasis. This characteristic suggests KLK6 as an attractive target for therapeutic interventions. However, inhibitors that specifically target KLK6 have not yet been reported, possibly because KLK6 shares a high sequence homology and structural similarity with other serine proteases and resists inhibition by many polypeptide inhibitors. The purpose of this study was to design and engineering a novel inhibitor for KLK6. Material and Method: In this study, first, the inhibitors were designed and investigated by using bioinformatics and molecular modeling. At this moment, the in vitro phase is being performed. In this phase, the KLK6 enzyme and a variety of engineered inhibitors will first be cloned, expressed and purified. Next, the activity of the KLK6 enzyme in the presence of inhibitors with the aid of a fluorogenic substrate will be investigated. Result: The new inhibitor, by forming more effective bonds, we hope that it will be able to inhibit the KLK6 enzyme in a stronger and more stable manner. Conclusion: In summary, Given the important role of KLK6 in a variety of cancers and chronic inflammatory diseases, we hope that the new inhibitor can be of help in treating these diseases. Of course, this needs further study. Keyword: Kallikrein6, Cancer, Inflammatory Diseases, Inhibitor
291 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 95599
Interaction between nut consumption with TCF7L2 polymorphisms and weight gain on metabolic syndrome incidence
Somayeh Hosseinpour-Niazi 1, Parvin Mirmiran 1, Fereidoun Azizi 2 1. Nutrition and Endocrine Research center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2. Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Purpose: The aim of this study was to evaluate the association between nut consumption and its various types and metabolic syndrome risk and to investigate whether both weight change and TCF7L2 polymorphisms modulate the association between of nut consumption and the risk of metabolic syndrome (MetS). Methods: We prospectively studied 1915 participants of the Tehran Lipid and Glucose study aged 19-74 years who were follow-up for dietary assessment using a validated, semi-quantitative food frequency questionnaire. TCF7L2 rs7903146 and rs12255372 were genotyped in 1008 participants. Multivariable adjusted Cox regression was used to estimate hazard ratios (HRs) for MetS events. Results: Mean age and BMI of participants (n = 1915, 40.5% male) was 36.5 ± 13 years and 25.6 ± 4.5 kg/m2, respectively and 591 new cases of MetS were documented during follow-up. The median nut consumption was 2.0 g/week (IQR: 1-4 g/week). Nut consumption and its constituents including fiber, polyphenol and PUFA was inversely associated with MetS in multivariable-adjusted models. Among various types of nuts, the multivariable-adjusted HRs of MetS were 0.73 (0.60-0.90) for walnuts, and 0.80 (0.65-0.98) for pistachios, compared with low intake. Among participants with weight gain ≥ 7 % during follow-up, consumption of nuts and walnuts, ≥ median intake, reduce risk of Mets by 25 % (0.75; 0.60-0.94) and 21 % (0.79; 0.64- 0.98), respectively. Among participants with weight gain < 7%, consumption of both nuts and walnuts, ≥ or < of median, reduce risk of MetS, compared with subjects with lower intakes of nuts and with weight change of ≥ 7 %. No significant interaction was found between consumption of nuts and its various types (per se) and TCF7L2- rs7903146 polymorphisms in relation to MetS incidence. Conclusion: Incorporating of nuts into dietary patterns prevent the risk of MetS especially among obese individuals, regardless of their TCF7L2 polymorphisms.
292 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 95743
Replacement in animal researches, a review article
Reza Mardani Veterinary Student, University of Shahr-e-kord, Shahr-e-kord, Iran
Introduction: Replacement methods are a vital one of the three Rs of animal testing alternatives because they completely replace the animal model with a non-animal method of testing. Alternatives to animal experiments are procedures which can completely replace the need for animal experiments, reduce the numbers of animals required, or diminish the amount of pain or distress suffered by the animals in meeting the essential needs of man and other animals.In this study, using google scholar and reviewing numerous articles, different alternatives to animal researches are considered. Description: Here are some alternatives to animal in scientific researches: “Organ – on – chips”, containing human cells grown in a state-of-the-art system to mimic the structure and function of human organs and organ systems, MatTek’s epiderm Tissue Model—a 3- dimensional, human cell–derived skin model that replicates key traits of normal human skin to assess skin allergy, devices made by German-base manufacturer VITROCELL are used to expose human lung cells in a dish to chemicals in order to test the health effects of inhaled substances, different tests that use human blood cells to detect contaminants in drugs that cause a potentially dangerous fever response when they enter the body, a wide range of sophisticated computer models that simulate human biology and the progression of developing diseases, Quantitative structure-activity relationships (QSARs) which are computer-based techniques that can replace animal tests by making sophisticated estimates of a substance’s likelihood of being hazardous, researches With human volunteers such as “microdosing” and Advanced brain imaging and recording techniques such as “MRI”, human-patient simulators such as “Trauma man”. Discussion & Conclusion: Given the global approach to respect for animal rights in medical research, it seems that in our country, it is necessary to pay more attention to animal rights by trying new technologies. Keywords: Replacement, Alternatives, Animal research, Animal rights
293 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 96020
Evaluation of the effects of extremely low-frequency electromagnetic fields on the viability of human gastric cancer (AGS) cell line
Fatemeh Alami Zavareh*1 , Dr.Soheila Abdi2, Dr.Malihe Entezari3 1. Author*, BSc in genetics, MSc student of Cellular and Molecular Science, Biology Department, Faculty of Advanced Science and Technology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran ([email protected]). 2. Corresponding Author, Department of Physics, Safadasht Branch, Islamic Azad University, Tehran, Iran. ([email protected]) . 3. Biology Department, Faculty of Advanced Science and Technology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran Iran ([email protected]). Introduction & Objectives: Gastric cancer (GC) is the fourth common cancer, and the second deadliest cancer in the world. There are many environmental and genetic factors involved in the promotion and progression of GC. One of the important environmental factors associated with cancer is extremely low-frequency electromagnetic fields (ELF- EMF). Epidemiological studies have suggested extremely low-frequency EMF (ELF- EMF) can increase the risk of developing cancer, such as leukemia, breast, and brain cancers. ELF-EMF can also affect on the Cellular and molecular processes. In this study , weinvestigated the effects of ELF- EMF on the cell viability of gastric cancer cell lines (AGS). Materials & Methods: Gastric cancer cell line (AGS) was obtained from Iran Genetic Resources Center.AGS cells were cultured under the exposure of 0.2 and 2 mT EMF during the one doubling time of cells continuously and discontinuously. Cell viability was evaluated by MTT assay method at the end of incubation. Cell viability in each group of the experiment was compared with control cell lines. Results: Results indicated that the cell viability of AGS cells was decreased to 10% and 56% when exposed to 0.2 and 2 mT EMFs continuously, in comparison to the control group. The cell viability of AGS cells also decreases to 4% and 49% under the exposure of 0.2 and 2 mT EMFs discontinuously. Conclusion: The results of the investigation indicated the exposure of AGS cell line to ELF-EMF could increase cell death, and this effect is dependent on the intensity of ELF- EMF and time and type of exposure. This issue could be considered in a comprehensive study to reduce cancer progression. Keywords: Electromagnetic field, cell viability, AGS cell line.
294 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 96080
Association between cholesterol ester transfer protein polymorphisms and dyslipidemia in children and adolescents
Motahar Heidari-Beni 1,Shaghayegh Haghjooy Javanmard2, Roya Kelishadi 3* 1- Assistant Professor of Nutrition, Department of Nutrition, Child Growth and Development Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran 2- Professor of Physiology,Department of Physiology, Applied Physiology Research Center, Isfahan University of Medical Sciences, Iran 3- Professor of Pediatrics, Department of Pediatrics, Child Growth and Development Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran
Introduction& Objective:Adverse levels of serum lipoprotein cholesterols among children and adolescents are important risk factors for coronary artery and early stages of atherosclerosis. Both genetic and environmental factors might influence lipid metabolism.One of the main proteins involved in lipoprotein metabolism is cholesterol ester transfer protein (CETP). This study aims to investigate association between CETP polymorphisms and dyslipidemia in Iranian children and adolescents. Materials &Methods:This study was conducted as a sub-study of the “school-based nationwide health survey” (CASPIAN-III). We randomly selected 750 samples from the whole blood samples. Real-time PCR and high resolution melt (HRM) analysis were performed to determine Taq1B (rs708272) and A373P (rs5880) polymorphisms. Results:Higher levels of HDL-C, and total cholesterol (TC) and lower levels of triglyceride and LDL-C were showed among those with Taq1B polymorphism. CT/TT genotype in Taq1B polymorphism showed a protective effect on dyslipidemia (OR= 0.12, 95%CI: 0.07-0.20). A373P polymorphism increased LDL-C and triglyceride levels anddecreased HDL-C and TC levels. G allele of A373P polymorphism increased the risk of dyslipidemia (OR=4.10, 95%CI: 2.14, 7.83) after adjusting the confounders. Conclusion:It seems that Taq1B polymorphism may have beneficial effects and A373P polymorphism have deleterious effect on dyslipidemia in Iranian children and adolescents. Keywords: Cholesteryl ester transfer protein, Single nucleotide polymorphisms, Dyslipidemia, Children, Adolescents
295 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 96157
Design of Nano bio-sensor based on graphene oxide for detection of mutation F508del in CFTR gene, leading to Cystic fibrosis
Zahra Emami1 , Mahmoud reza Sohrabi1, Roghayeh Abbasalipourkabir2 1. Department of Chemistry, Islamic Azad University, North Tehran Branch, Tehran, Iran 2. Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Introduction and objectives: Cystic fibrosis (CF) is one of the most common autosomal recessive diseases of Caucasian population which is caused by mutations in the Cystic Fibrosis Trans membrane conductance Regulator (CFTR) gene. The most common mutation for CF is referred to ΔF508, with a frequency of 86.4%. Cystic fibrosis is a multiple organ disease, which was affected on many organs such as lung, liver, pancreas, intestine bones etc. In the present work, a new nano-biosensor based on graphene oxide (GO) and 5-carboxy fluorescein (FAM) labeled DNA introduced for detecting deletion mutation in cystic fibrosis. Materials and methods: The graphite flake was purchase from Merck. According
modified hummers method it was oxidized with H2SO4, HNO3 and KMnO4, under 55.0 °C for 12 h. Oligonucleotide sequences used in this study are as follows: 1) fDNA: 5- carboxy fluorescein fluorophore labeled at the 5′ terminal (the sequence of CFTR gene without any mutation located at codon 508). 2) cDNA the sequence of complementary target. 3) mDNA: the sequence of mismatch DNA possessing 21 bases deletion mutation at exon18 of CFTR gene. All nucleotides were purchased from BIONEER Co. (South Korea). Results: fDNA was quenched by GO, while adding cDNA caused double-stranded DNA formed and fluorescence (FL) emission was recovered again. By the optimum parameters in hand, the sensitivity of the designed probe for the detection of cDNA was investigated using various concentration of cDNA. It was deduced that, mDNA was unable to joint with fDNA and pick it up from GO surface so, FL emission was remain quenched. Conclusion: A new Nano bio-sensor was introduced for detection of Cystic fibrosis by studding on mutation located at codon 508, ΔF508. Key words: Cystic fibrosis, graphene oxide, mutation, Nano-biosensor
296 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 96256
Scar-free skin healing
Mohadeseh Ghorbani¹*, farzaneh jabbari², Reza Salarian1 1- Department of Biomedical Engineering, Maziar Higher Education Institute, Royan, Iran 2- Biomedical engineering, Materials and Energy Research Center
Introduction: Every scar tells a story — about the time you fell from your bike or the day you burnt your hand. Every scar also tells about the body’s amazing ability to heal itself. But it’s not perfect. Unfortunately, many times when our skin is wounded, the cut isn’t clean, the healing conditions are not ideal and we end up with a scar. The tissue will try to reorganize, and the scar may appear to soften, but the skin may never completely return to its original state — particularly if the cut extends beyond the epidermis, the skin’s outer layer. Skin cells regenerate from the bottom up, with a turnover time of roughly one month. We can think of the epidermis as a staircase, where the bottom stair is the base of the epidermis and the top stair is exposed to the air. New epidermal cells are formed by cell division at the bottom of the staircase. To make room for these new cells, the older epidermal cells are pushed upward toward the top of the staircase. The oldest cells die and fall off. Description: In the context of this overview, 25 articles were used between the years 2010 through the end of 2018 using the Keywords of PubMed, Google scholar search engines. Discussion and conclusion: A number of potential therapies have been developed to reduce scar formation in cutaneous wounds based on differences between the process of adult and fetal wound healing. The ideal approach to eliminate scar formation after skin injury is to use a pro-regenerative matrix along with growth factors and cell types that induce regeneration rather than repair. Keywords: Skin, Wound Healing, Scar
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 96854
Gene therapy
Nazanin Nasehi, Department of Cell and Molecular biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan
Introduction: Gene therapy is bringing new treatment options to multiple fields of medicine. Gene therapy involves identifying appropriate DNA sequence and cell type and then developing suitable ways in which to get enough of the DNA into these cells. This idea has many goals, including the treatment of both inherited and acquired diseases. In 1990 the first clinical trials for gene therapy for adenosine deaminase (ADA) deficiency were carried out on two young girls at the National Institutes of Health. Results from currently ongoing clinical trials will further improve our understanding of gene. Description: There is a wide range of gene therapy methods like immunotherapy, oncolytic virotherapy, and gene transfer. In gene transfer, a new gene can be sent into a cancerous cell or the surrounding tissue to cause cell death or slow the growth of the cancer. Genome editing can be performed on cells ex vivo or in vivo to modify cells. Genes can be inserted into cancer cells by carriers such as viruses to inhibit carcinogenic oncogenes, produce specific proteins, or stop tumor-stimulating genes. Currently, gene therapy is being used to create recombinant cancer vaccines. Oncolytic gene therapy vectors are generally viruses that have been genetically engineered to target and destroy cancer cells. Oncolytic vectors are designed to infect cancer cells and induce cell death through the propagation of the virus, expression of cytotoxic proteins, and cell lysis. This method can be used to treat somatic and germ cells. Discussion and conclusion: Gene therapy is one of the most complex drugs ever developed. The biggest challenge facing all viral vectors is the immune response of the host. Research is also underway to see if this treatment can reduce the side effects of various anticancer drugs by increasing stem cell resistance.
298 Page
International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 97595
Hanging drop culture enhances differentiation of human adipose-derived stem cells into anterior neuroectodermal cells using small molecules.
Noushin Amirpour1 and Hossein Salehi1 1. Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract: Inspired by in vivo developmental process, several studies were conducted to design a protocol for differentiating of mesenchymal stem cells into neural cells in vitro. Human adipose-derived stem cells (hADSCs)as mesenchymal stem cells are a promising source for this purpose. At current study, we applied a defined neural induction medium by using small molecules for direct differentiation of hADSCs into anterior neuroectodermal cells. Anterior neuroectodermal differentiation of hADSCs was performed by hanging drop and monolayer protocols. At these methods, three small molecules were used to suppress the BMP, Nodal, and Wnt signaling pathways in order to obtain anterior neuroectodermal (eye field) cells from hADSCs. After two and three weeks of induction, the differentiated cells with neural morphology expressed anterior neuroectodermal markers such as OTX2, SIX3, β-TUB III and PAX6. The protein expression of such markers was confirmed by real time, RT-PCR and immunocytochemistry methods According to our data, it seems that the hanging drop method is a proper approach for neuroectodermal induction of hADSCs. Considering wide availability and immunosuppressive properties of hADSCs, these cells may open a way for autologous cell therapy of neurodegenerative disorders.
299
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 99207
Medical Devices and Artificial Organs
Cheshmeh Habibi Roudsary1*, Farzaneh Jabbari2, Reza Salarian1 1. Department of Biomedical engineering (Biomaterial), Maziar Higher Education Institute, Royan Iran 2. PhD Biomedical engineering (Biomaterial), Nanotechnology and Advance Material Department, Materials and Energy Research Center (MERC), Alborz Iran
Background and objective: One important goal for Regenerative Medicine is to develop and define methods that will maintain, improve or even restore the function of diseased organs. The growing need for these technologies is substantial. Search method: In the context of this overview, 14 articles were used between the years 2010 through the end of 2018 using the Keywords of PubMed, Google scholar search engines. Results: Improved health care has resulted in an increased life span for the general population and, when coupled with a growing shortage of donor organs, makes it clear that organ assistance and substitution devices will play a larger role in managing patients with end-stage disease by providing a bridge to recovery or transplantation. The good news is that the field of medical device and artificial organ development is redefining what is believed to be possible for augmenting or replacing organ function. Once constructed only of synthetic components, these devices may now be either fully artificial or bio artificial- so-called “biohybrid organs” – a combination of biologic and synthetic components, often incorporating multiple technologies involving sensors, new biomaterials, and innovative delivery systems. Conclusion: Some devices can provide assistance while new therapies incorporating stem cells, gene therapy, or engineered tissues are employed to repair or replace the damaged organ. Until these interesting therapies can be introduced and generated, medical devices must play a crucial role in facilitating organ regenerating and, maybe, organ salvage through natural repair process. If organ regeneration is impossible, artificial organs can provide a substitute for natural organs. Keywords: Medical devices, Artificial organs, Tissue regeneration
300
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International Congress of Isfahan Biomedical Sciences ICIBS – 2020 26th September to1st October
Type of article present: Poster ID Code: 99742
Molecular docking of five colon cancer drugs with the erbb1 receptor
Fatemeh Karimi1, Rahman Emamzadeh1*, Mahboobeh Nazari2
1. Department of cell and molecular biology and Microbiology, Faculty of Biological Sciences and Technologies, University of Isfahan, Isfahan, Iran. * [email protected]
2. Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Abstract: Colon cancer (CC) is the third most common type of cancer among women and men. Most causes of CC are due to age and lifestyle, with only a small number of cases due to underlying genetic disorders. Signs and symptoms of CC include blood in the stool, weight loss and altered bowel movements. Studies have shown that kinase tyrosine receptors play an important role in CC. Among these receptors, the epidermal growth factor receptor (EGFR) plays a more important role in tumorigenesis, tumor processes in colon cancer and activation of intracellular signaling pathways. Anti-EGFR drugs are used to reduce and treat the symptoms and symptoms of colon cancer, such as afatinib, brigatinib, dacomitinib, erlotinib and dasatinib. In this study, the binding energy of drugs to the EGFR receptor has been investigated by in silico studies. The receptor pdb sequence (G719S) was obtained from the protein Data Bank (PDB) and the three-dimensional pdb sequence of the drug was obtained using the drug bank server, then evaluated using Autodock software for binding energy and ligand efficiency between the drug and the receptor. The results revealed that erlotinib had a more stable binding to the receptor because the binding energy was lower than the other drugs mentioned. Keywords: Colon cancer, EGFR, Erlotinib, Autodock software
301 Page
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Isfahan University of Medical Sciences was founded by Great Avicenna (Ibn_sina), under the patronage of the Kakouid ruler Alla-al-Dowleh in 1024/1025. It soon became one of the leading educational centers of the historic Muslim world. Isfahan University of Medical Sciences (IUMS), was established in 1946, as the Isfahan Higher School of Health Education. IUMS is one of the world's oldest universities. It is also one of the most prestigious Iranian medical schools.
Isfahan University of Medical Sciences is located on a 400-hectare campus adjacent to the University of Isfahan in the southwestern corner of the City of Isfahan. It lies on the heights of Mount Soffeh foothills. Hence, the University enjoys a panoramic view of the city. Faculty buildings with an overall area of about 102,000 square meters are located among the spectacularly landscaped green areas of the university campus, some of which with historical and architectural value. More than 30% of the University buildings are used for educational purposes, and the rest is in use by the administrative, recreational and general services sectors. Hezar-Jerib highway runs alongside the eastern border of the University campus and is the southern gateway to the city.