Proceedings, US Department of Agriculture Interagency Gypsy Moth
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(i,·~,�:;,,... United States Ll,.j)� Department of �} Agriculture Forest Service PROCEEDINGS Northeastern Forest Experiment Station u. s. Department of Agriculture General Technical Report NE-213 lnteragency Gypsy Moth l/ Research Forum 1995 �· {'{;;; . --,,,,, ;:�� W _ p I •I Most of the abstracts were submitted on floppy disk and were edited to achieve a uniform format and type face. Each contributor is responsible for the accuracy and content of his or her own paper. Statements of the contributors from outside the U. S. Department of Agriculture may not necessarily reflect the policy of the Department. Some participants did not submit abstracts, so they have not been included. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an officialendorsement or approval by the U. S. Department of Agriculture or the Forest Service of any product or service to the exclusion of others that may be suitable. Remarks about pesticides appear in some technical papers contained in these proceedings. Publicationof these statements does not constitute endorsement or recommendation of them by the conferencesponsors, nor does it imply that uses discussed have been registered. Use of most pesticides is regulated by State and Federal Law. Applicable regulations must be obtained from the appropriate regulatory agencies. CAUTION: Pesticidescan be injurious to humans, domestic animals, desirable plants, and fish and other wildlife--if they are not handled and applied properly. Use all pesticides selectively and carefully. Follow recommended practices given on the label foruse and disposal of pesticides and pesticide containers. ACKNOWLEDGMENTS Thanks to Dr. Mark J. Twery for providing the cover and title page design. Proceedings U.S. Department of Agriculture Interagency Gypsy Moth Research Forum 1995 January 17-20, 1995 Loews Annapolis Hotel Annapolis, Maryland Edited by Sandra L. C. Fosbroke and Kurt W. Gottschalk Sponsored by: Forest Service Research Forest Service State and Private Forestry Agricultural Research Service Animal and Plant Health Inspection Service Cooperative State Research Service �:.·.:.·.� 1z�1' FOREWORD This meeting was the sixth in a series of annual USDA Interagency Gypsy Moth Research Forums thatare sponsoredby the USDA Gypsy Moth Research and Development Coordinating Group. The Committee's original goal of fosteringcommun ication and an overview of ongoing research has been continuedand accomplished in this meeting. The proceedings document the effortsof many individuals: those who made the meeting possible, those who made presentations, and those who compiled and edited the proceedings. But more than that, the proceedings illustrate the depthand breadth of studies being supported by the agencies and it is satisfying,indeed, that all of this can be accomplished in a cooperative spirit. USDA Gypsy Moth Research and Development CoordinatingGroup R. Faust, Agricultural Research Service (ARS) N. Leppla, Animal and Plant Health Inspection Service (APHIS) R. Riley, Cooperative State Research Service (CSRS) T. Hofacker, Forest Service-State and Private Forestry (FS-S&PF) M. McFadden, Forest Service-Research (FS-R), Chairperson 1995USDA Interagency GypsyMoth ResearchForum ii USDA Interagency Gypsy Moth Research Forum January 17-20, 1995 Loews Annapolis Hotel Annapolis, Maryland AGENDA Tuesday Afternoon, January 17 REGISTRATION POSTER DISPLAY SESSION I WELCOME MIXER Wednesday Morning,January 18 PLENARY SESSION ......................... Moderator: M.McFadden, USDA-FS Changes in Funding and Direction of Science Welcome Michael McManus, USDA-FS Research and Service Programs of the USDA-ARS European Biological Control Laboratory in Montpellier, France Lloyd Knutson, USDA-ARS Agricultural Research Service Program Strategies and Priorities Edward Knipling, USDA-ARS Inside the Animal and Plant Health Inspection Service Charles Schwalbe, USDA-APHIS Changes in Forest Service Research: What Lies Ahead? Robert Lewis, Jr., USDA-FS Wednesday Afternoon, January 18 CONCURRENT SESSION A ................. Moderator: E.Delfosse, USDA-APHIS ConcernsAbout Biological Control Agents and Non-Target Lepidoptera Presenters: A. Hajek, Cornell University; L. Solter, Illinois Natural History Survey; J. M. Scriber, Michigan State University; J.Miller, Oregon State University; J.Maddox, Illinois Natural History Survey iii 1995 USDA Interagency Gypsy Moth ResearchForum CONCURRENT SESSION B .. .. .. .. .. .. Moderator: R.M.Muzika, USDA-PS Potpourri Presenters: D.Leonard, USDA-PS and A.Sharov, Virginia Polytechnic Institute & State University;-R.Hicks, Jr., West Virginia University; D.Gray, Virginia Polytechnic Institute & State University; A.Liebhold, USDA-PS; R.Whitmore, West Virginia University; R. A. Smith, Abbott Laboratories POSTER DISPLAY SESSION II Thursday Morning,January 19 GENERAL SESSION .. .. .. .. .. .. Moderator: V. Mastro, USDA-APHIS Asian Gypsy Moth Presenters: K.Gamer, D. Schreiber and J.Slavicek, USDA-PS; D.Frashe r, USDA-APHIS; M. Keena, USDA-PS; R. Carde, University of Massachusetts; W.Wall ner, USDA-PS; P. Schaefer,USDA-ARS; T. McGovern, USDA-APHIS Thursday Afternoon,January 19 GENERAL SESSION .......................... Moderator: K. Thorpe,USDA-ARS Entomophaga maimaiga: A Fungus Among Us Presenters: A.Hajek, CornellUniversity; R.Weseloh, Connecticut Agricultural Experiment Station; J.Elkinton, University of Massachusetts; L. Bauer, USDA-PS; S.Walsh, University of Toronto GENERAL SESSION ......................... Moderator: R.Fuester, USDA-ARS Gypsy Moth Biological Control Activitiesin Europe Presenters: E. A.Cameron, Pennsylvania State University; J. Novotny, Forest Research Institute, Slovak Republic;M. McManus, USDA-PS Friday Morning, January 20 GENERAL SESSION ........................ Moderator: N.Le ppla, USDA-APHIS The Increasing Significance of Biological Control and an Overview of Regulations Governing Biological Control Organisms Presenters: E. Delfosse,USDA-APHIS; K.Lakin, USDA-APHIS; L. Turner,US-EPA; J.Brooks, USFWS 1995USDA Interagency Gypsy Moth Research Forum iv GENERAL SESSION ........................... Moderator: R.Reardon, USDA-FS Future Directions in Virus Research Presenters: V.D'Amico, University of Massachusetts; J. Podgwaite, USDA-FS; J. Slavicek, USDA-FS; M.McFadden, USDA-FS CONTENTS PLENARY PRESENTATIONS Programs of the European Biological Control Laboratory, USDNARS, Montpellier, France .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 1 L. Knutson WORKSHOP SUMMARY Introductionto the Session: "The Increasing Significance of Biological Control and an Overview of Regulations Governing Biological Control Organisms" ............... 9 N. C.Leppla and E.S. Delfosse Guidelines for obtaining a plant pest permit fromthe U.S.Department of Agriculture .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 20 K. R.Lakin Obtaining EPA approval to test or commercialize microbial and/or biochemical pesticides .................................................... 28 M. L. Mendelsohn and P. 0.Hutton U.S.Fish and WildlifeService regulations governingthe collection, possession, and transportation of wildlifeand plants, as related to the scientific community ........... 31 U.S. Fish and Wildlife Service, Division of Law Enforcement ABSTRACTSAND PAPERS OF PRESENTATIONS AND POSTERS The Russian and Ukrainian literature on the gypsy moth .......................... 48 Y.N. Baranchikov, G.N. Nikitenko, and M. E. Montgomery Suitability of foreign tree species forLymantria mathura Moore .................... 49 Y.N. Baranchikov, T. Vshivkova, and M.E. Montgomery Studies on the transmission of an exotic microsporidium that infects the gypsy moth .... 50 L.S. Bauer, D.L. Miller, D.W. Onstad, J.V. Maddox, and M.L. McManus v 1995USDA Interagency Gypsy Moth ResearchForum Dynamics and impact of Entomophaga maimaiga introduced into gypsy moth populations in Michigan .................................................. 52 L. S.Bauer, D. R. Smitley, A. E. Hajek, F. J. Sapio, and R. A. Humber Status of mass-reared gypsy moths: protocol ................................... 54 G. L. Bernon Identification of the Lymantria dispar nuclear polyhedrosis virus 25K gene ........... 55 D. S. Bischoffand J.M. Slavicek Sequence characterization and temporal expression of an early gene in the Lymantria dispar nuclear polyhedrosis virus ........................................... 56 D. S. Bischoffand J.M. Slavicek Corsica, gypsy moth, and parasitoids: challenges and opportunities .................. 57 E.A. Cameron and F. Herard A fieldassessment of theeffects of Bacillus thuringiensis on non-target Lepidoptera: light trap sampling ...................................................... 59 J. L. Carter, J.W. Peacock, L. Neale, and S.E. Talley Gypsy moth management in non-forestsettings: 1994 fieldand laboratory studies ...... 60 S. P. Cook, R.E. Webb, and K. W. Thorpe A fieldtest of genetically engineered gypsy moth NPV ........· ..... .............. 61 V. D' Amico, J. S.Elkinton, H. A. Wood, J.D. Podgwaite, M. L.McManus, J. Slavicek, and J.P.Burand Gypsy moth defoliation in Coastal Plain pine-hardwood stands .................... 62 C. B.Davidson and J. E.Johnson Litterfall dynamics in gypsy moth defoliated pine-hardwood stands ................. 63 C. B. Davidson and J.E. Johnson Effectof repeated treatments of Bacillus thuringiensis against gypsy moth populations: initial survey ................................................. 64 N.R. Dubois, M.A. Keena, P. Huntley, and D. Newman