bioRxiv preprint doi: https://doi.org/10.1101/537464; this version posted January 31, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. “Multiple human adipocyte subtypes and mechanisms of their development.” So Yun Min1,2, Anand Desai1, Zinger Yang2, Agastya Sharma1, Ryan M.J. Genga1,2,4, Alper Kucukural3, Lawrence Lifshitz1, René Maehr1,4, Manuel Garber1,3, Silvia Corvera1,*. 1Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01655, USA 2Graduate School oF Biomedical Sciences, University of Massachusetts Medical School, Worcester, MA 01655, USA 3Program in BioinFormatics, University of Massachusetts Medical School, Worcester, MA 01655, USA 4Department of Medicine, Diabetes Center of Excellence, University of Massachusetts Medical School, Worcester, MA 01655, USA *Corresponding author:
[email protected] KEY WORDS: ADSC, mesenchymal stem cells, pre-adipocyte, adipocyte diFFerentiation, adipocyte development, leptin, adiponectin, iron, brown adipocyte, beige adipocyte, thermogenic Fat, obesity, diabetes SUMMARY Human adipose tissue depots perform numerous diverse physiological functions, and are diFFerentially linked to metabolic disease risk, yet only two major human adipocyte subtypes have been described, white and “brown/brite/beige.” The diversity and lineages oF adipocyte classes have been studied in mice using genetic methods that cannot be applied in humans. Here we circumvent this problem by studying the Fate oF single mesenchymal progenitor cells obtained From human adipose tissue. We report that a minimum oF Four human adipocyte subtypes can be distinguished by transcriptomic analysis, specialized for functionally distinct processes such as adipokine secretion and thermogenesis.