molecules Article 1,6-Dehydropinidine Is an Abundant Compound in Picea abies (Pinaceae) Sprouts and 1,6-Dehydropinidine Fraction Shows Antibacterial Activity against Streptococcus equi Subsp. equi Virpi Virjamo 1,2,*, Pia Fyhrquist 3, Akseli Koskinen 1, Anu Lavola 1, Katri Nissinen 1 and Riitta Julkunen-Tiitto 1 1 Natural Product Research Laboratory, Department of Environmental and Biological Sciences, University of Eastern Finland, P.O. Box 111 Joensuu, Finland;
[email protected] (A.K.); anu.lavola@uef.fi (A.L.); katri.nissinen@uef.fi (K.N.); riitta.julkunen-tiitto@uef.fi (R.J.-T.) 2 School of Forest Sciences, University of Eastern Finland, P.O. Box 111 Joensuu, Finland 3 Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, P.O. Box 56 Helsinki, Finland; pia.fyhrquist@helsinki.fi * Correspondence: virpi.virjamo@uef.fi Academic Editor: Natalizia Miceli Received: 11 September 2020; Accepted: 4 October 2020; Published: 6 October 2020 Abstract: Knowledge about the defensive chemistry of coniferous trees has increased in recent years regarding a number of alkaloid compounds; in addition to phenolics and terpenes. Here, we show that Norway spruce (Picea abies (L.) H. Karst.), an important boreal zone tree species; accumulates 1,6-dehydropinidine (2-methyl-6-(2-propenyl)-1,6-piperideine) in its needles and bark. We reanalyzed previously published GC-MS data to obtain a full picture of 1,6-dehydropinidine in P. abies. 1,6-dehydropinidine appeared to especially accumulate in developing spring shoots. We used solid-phase partitioning to collect the alkaloid fraction of the sprouts and thin-layer chromatography to purify 1,6-dehydropinidine.