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ISSN. 2087-2852 E-ISSN. 2338-1655 The Journal of Experimental Life Science Discovering Living System Concept through Nano, Molecular and Cellular Biology Editorial Board Chief Editor Dian Siswanto, S.Si., M.Sc., M.Si., Ph.D Editorial Board Aida Sartimbul, M.Sc. Ph.D - UB Sukoso, Prof. MSc. Ph.D-UB Adi Santoso, M.Sc. Ph.D - LIPI Etik Mardliyati, Dr. - BPPT Nurul Taufiq, M.Sc. Ph.D - BPPT Soemarno, Ir., MS., Dr., Prof. - UB Arifin Nur Sugiharto, M.Sc. Ph.D -UB M. Sasmito Djati, Ir., MS., Dr., Prof. - UB Reviewers Aris Soewondo, Drs., M.Si – UB Andi Kurniawan, S.Pi, M.Eng, D.Sc. - UB Muhaimin Rifai, Ph.D., Prof. – UB Suharjono, MS., Dr. - UB Sri Rahayu, M.Kes., Dr. – UB Attabik Mukhammad Amrillah, S.Pi., M.Si - UB Dina Wahyu Indriani, STP., MSc. – UB Indah Yanti, S.Si., M.Si. - UB Widodo, S.Si., M.Si., Ph.D MED Sc., Prof. – UB Yoga Dwi Jatmiko, S.Si., M.App.Sc., Ph.D. - UB Moch. Sasmito Djati, Ir., MS, Dr., Prof. – UB Catur Retnaningdyah, Dra,. M.Si., Dr. - UB Nia Kurniawan, S.Si.,MP.,D.Sc – UB Mohammad Amin, SPd., M.Si., Dr. Agr., Prof. - UM Retno Mastuti, Ir., M.Agr.Sc., D.Agr.Sc – UB Bagyo Yanuwiadi, Dr. – UB Sutiman Bambang S., Dr., Prof. - UB Muhamad Firdaus, Ir., MP., Dr. - UB Irfan Mustafa, S.Si.,M.Si.,Ph.D - UB Yuni Kilawati, S.Pi., M.Si., Dr. – UB Mufidah Afiyanti, Ph.D. – UB Amin Setyo Leksono, S.Si.,M.Si.,Ph.D - UB Estri Laras Arumingtyas, Dr., Prof. – UB Editorial Assistant Jehan Ramdani Haryati, S.S.i, M.Si. Illustrator M. Qomaruddin, S.Si., M.M. Address The Journal of Experimental Life Science Building B, 1st Floor, Postgraduate School, University of Brawijaya Jl. Mayor Jenderal Haryono 169, Malang, 65145 Telp: (0341) 571260 ; Fax: (0341) 580801 Email: [email protected] Web: http://www.jels.ub.ac.id J. Exp. Life Sci. Vol. 9 No. 2, 2019 pages. 65-146 ISSN. 2087-2852 E-ISSN. 2338-1655 The Journal of Experimental Life Science Table of Content Phytochemical Screening and Antimicrobial Activity of Roselle (Hibiscus sabdariffa L.) Flower Extract Against Aeromonas hydrophila (Sitti Khairul Bariyyah, Arief Prajitno, Ating Yuniarti) ....................................................................................... 65-69 DOI: http://dx.doi.org/10.21776/ub.jels.2019.009.02.01 Phytochemicals and The Ability of Plantago major Linn. Extract to Inhibit The growth of Aeromonas hydrophila (Annisa Farhana Dewi, Arief Prajitno, Ating Yuniarti) ....................................................................................... 70-75 DOI: http://dx.doi.org/10.21776/ub.jels.2019.009.02.02 Potential of Dayak Onion (Eleutherine palmifolia (L) Merr) Extract As Antibacterial Against Pseudomonas fluorescens (Immaria Fransira, Uun Yanuhar, Maftuch Maftuch) .......................................................................................... 76-80 DOI: http://dx.doi.org/10.21776/ub.jels.2019.009.02.03 Growth and Development of Rhyzopertha dominica Fabricius (Coleoptera: Bostrichidae) on White, Red and Black Rice (Dewi Fajarwati, Ludji Pantja Astuti, Toto Himawan) ........................................................................................ 81-89 DOI: http://dx.doi.org/10.21776/ub.jels.2019.009.02.04 The Effect of Biogas Waste Doses (Sludge) of Cow Manure on the Growth and the Yield of Eggplant (Solanum melongena L.) (Ach Fauzan Mas'udi, Hidayat Bambang Setyawan) ............................................................................................. 90-96 DOI: http://dx.doi.org/10.21776/ub.jels.2019.009.02.05 Optimization of Time and Temperature Gelatin Extraction from Pink Perch (Nemipterus bathybius) Head using Response Surface Methodology (RSM) (Ulfatul Mardiyah, Simon Bambang Widjanarko, Kiki Fibrianto) .................................................................. 97-104 DOI: http://dx.doi.org/10.21776/ub.jels.2019.009.02.06 Effects of Monosodium Glutamate Oral Administration on LH and Testosterone Levels in Serum of Adult Male Rats (Rattus norvegicus) (Riska Annisa, Moch. Sasmito Djati, Sri Rahayu) .................................................................................................... 105-109 DOI: http://dx.doi.org/10.21776/ub.jels.2019.009.02.07 Dietary Rice Bran Plays A Significant Role in the Hepatoprotective Effect in Hypercholesterolemic Rats (Yulianti Antula, Dolly Irnawati Neno, Arie Srihardyastutie, Chanif Mahdi) ............................................ 110-115 DOI: http://dx.doi.org/10.21776/ub.jels.2019.009.02.08 The Effect of Monosodium L-Glutamate (MSG) Treatment for Short and Long Terms to The Semen Quality of Adult Male Rats (Ivakhul Anzila, Agung Pramana Warih Marhendra, Sri Rahayu) .................................................................. 116-121 DOI: http://dx.doi.org/10.21776/ub.jels.2019.009.02.09 The Study of Growth and Its Polyembryonic Properties of Porang Seeds (Amorphophallus muelleri Blume) from Various Fruit Colors in Different Planting Media (Imaniah Bazlina Wardani, Nunung Harijati, Retno Mastuti) .......................................................................... 122-127 DOI: http://dx.doi.org/10.21776/ub.jels.2019.009.02.10 Alcohol Intake Investigation of Adult Rats Based on Sperm Parameters (Choirul Anam, Agung Pramana Warih Marhendra, Sri Rahayu) ................................................................... 128-132 DOI: http://dx.doi.org/10.21776/ub.jels.2019.009.02.11 J. Exp. Life Sci. Vol. 9 No. 2, 2019 pages. 65-146 Potential of Olive Oil Extract (Olea europaea) For Affecting Lipid Profile, Lipid Oxidative and Fatty Liver on Hiperlipemic Rats (Rattus norvegicus) (Annisa Hanifwati, Agung Pramana Warih Marhendra, Aulanni’am Aulanni’am) ................................. 133-138 DOI: http://dx.doi.org/10.21776/ub.jels.2019.009.02.12 Heavy Metal (Pb) and Its Bioaccumulation in Red Algae (Gracilaria sp.) At Kupang Village, Jabon Sub-District, Sidoarjo District (Yatris Rambu Tega, Endang Yuli Herawati, Yuni Kilawati) ............................................................................. 139-146 DOI: http://dx.doi.org/10.21776/ub.jels.2019.009.02.13 J. Exp. Life Sci. Vol. 9 No. 2, 2019 pages. 65-146 Phytochemical Screening & Antimicrobial Activity of Roselle 65 (Bariyyah, et al) Phytochemical Screening and Antimicrobial Activity of Roselle (Hibiscus sabdariffa L.) Flower Extract Against Aeromonas hydrophila Sitti Khairul Bariyyah*1, Arief Prajitno2, Ating Yuniarti2 1*Master Program of Aquaculture, Faculty of Fisheries and Marine Science, University of Brawijaya, Malang, Indonesia 2Faculty of Fisheries and Marine Science, University of Brawijaya, Malang, Indonesia Abstract Medicinal plants as an antimicrobial agent may provide an alternative way to replace the use of antibiotics to control disease agents in aquaculture. Roselle flowers (Hibiscus sabdariffa L.) has been used in many sectors as a source of functional food, natural coloring agents as well as antimicrobial agents. The objectives of this study were to evaluate the phytochemical compound in methanolic extract of roselle flower and their antimicrobial activity against Aeromonas hydrophila. The phytochemical composition of roselle flower was evaluated using phytochemical screening and FTIR. While the antimicrobial activity was performed by using the disc diffusion agar and co-culture with A. hydrophila. The results of phytochemical screening confirmed the presence of alkaloids, flavonoids, saponins, steroids, triterpenoids, and tannins. The results of FTIR revealed that Roselle flower extract had the main phenolic compounds. The result of disc diffusion and co-culture method indicated that the roselle flower extract had antibacterial activity against A. hydrophila. This antibacterial activity depended on the concentration applied. Keywords: antibiotics, Co-culture, Disk diffusion, flavonoid, Hibiscus sp. INTRODUCTION* several strain of bacteria such as Micrococcus Aeromonas hydrophila is a common bacteria luteus, Serratia mascences, Staphylococcus in freshwater habitats throughout the world [1]. aureus, Staphylococcus epidermidis, Salmonella It cause diseases both in human and animals enterica, Pseudomonas aeruginosa, Klebsiella including fish and shrimp [2]. Bacterial infections pneumonia, Proteus vulgaris, Escherichia coli, and with Aeromonas hydrophila as a disease agent Bacillus cereus [14,15]. Yet, no studies found to have resulted in heavy losses up to 80% and reveal the antimicrobial activities against A. economic loss to fish farmers [3,4]. hydrophila as a bacterial disease agent in Usually, many farmers have used antibiotics aquaculture. Therefore, this study was intended to control this disease. However, antibiotics have to evaluate the phytochemical screening and become the major factor for the emergence and antimicrobial activity of Roselle flower extract on dissemination of multi-drug-resistant strains of A. hydrophila indigenous aquatic. several groups of microorganisms [5]. An effort MATERIAL AND METHODS to overcome the negative effects of antibiotic Preparation of Roselle flower Extract application is the use of medicinal plants. Plants Roselle flower (H. sabdariffa L.) was are rich in a wide variety of secondary originated from house of medicinal plant, Batu, metabolites such as tannins, alkaloids, and East Java. The flowers were air-dried and flavonoids, which have been found in vitro to grounded into fine powder using an electric have antimicrobial properties [6]. There have blender. Extraction process was performed based been several reports on the antimicrobial activity on the methods of [16] with some modification. of different herbal extracts [7–9].