Pournaras, Spyros Date of Birth: November 4, 1969 Place: Larissa 1

SPYROS POURNARAS, SHORT CURRICULUM VITAE, OCTOBER 2010

Name: Pournaras, Spyros Date of Birth: November 4, 1969 Place: Larissa 1993: Graduate, Medical School, Aristotle University of Thessaloniki (A.U.T.) 1995-1999: Registrar, Department of Microbiology, Medical School, A.U.T. 1999: Specialty of Medical Microbiology 2000: PhD, Medical School, A.U.T. 2001: Lecturer, Department of Microbiology, Medical School, University of Thessaly 2007: Assistant Professor, Department of Microbiology, Medical School, University of Thessaly 2009: Director, Department of Clinical Chemistry, Medical School, University of Thessaly

PUBLICATIONS IN PEER-REVIEWED JOURNALS (SCI): 96 1st AUTHOR: 23 2nd AUTHOR: 18 3rd AUTHOR: 14 LAST AUTHOR: 11 OTHER POSITION: 30 CORRESPONDING AUTHOR: 18

SUM IMPACT FACTOR (IF): 379,350 MEAN IMPACT FACTOR (IF): 3,95 H-Index: 15 CITATIONS (ISI, SCOPUS 10/10/2010): 970

AUTHOR OF CHAPTERS IN INTERNATIONAL MEDICAL BOOKS: 2 Chapters

MEMBER OF THE EDITORIAL BOARD OF PEER-REVIEWED JOURNALS: 1. Journal of Antimicrobial Chemotherapy (IF 4,352) 2

2. Chemotherapy Research and Practice

REVIEWER OF PEER-REVIEWED JOURNALS: i) Journal of Antimicrobial Chemotherapy ii) Journal of Medical Microbiology iii) BMC Microbiology iv) BMC Infectious Diseases v) Indian Journal of Cancer vi) Joural of Medical Virology vii) Clinical Microbiology and Infection viii) Photomedicine and Laser Surgery ix) Recent Patents On Anti-Infective Drug Discovery x) Clinical Chemistry xi) Expert Opinion in Investigating Drugs xii) Scandinavian Journal of Infectious Diseases xiii) Current Drug Targets xiv) Applied and Environmental Microbiology xv) Antimicrobial Agents and Chemotherapy xvi) Journal of Clinical Microbiology xvii) Journal of Infection xviii) Microbiology

Impact Factors, IF (2009)

Journal IF Publications No Sum IF

J Antimicrob Chemother 4,352 25 108,800 J Clin Microbiol 4,162 15 62,430 Antimicrob Agents Chemother 4,802 8 38,416 Eur J Clin Microbiol Infect Dis 2,605 3 7,815 Diagn Microbiol Infect Dis 2,451 1 2,451 J Clin Virol 3,124 1 3,124 Gynecol Oncol 3,733 1 3,733 J Clin Lab Anal 0,855 1 0,855 Occup Med 1,124 1 1,124 Lett Appl Microbiol 1,640 2 3,280 Fertil Steril 3,970 4 15,880 Hum Reprod 3,859 1 3,859 Comp Immunol 2,986 2 5,972 Microbiol Infect Dis J Infect 3,060 1 3,060 Chemotherapy 2,028 1 2,028 Inf Control Hosp Epidemiol 2,768 2 5,536 Int J Antimicrob Agents 3,032 3 9,096 Journal of Medical Microbiology 2,272 1 2,272 Clin Microbiol Infect 4,014 5 20,070 Epidemiol Infect 2,362 2 4,724 Emerg Infect Dis 6,794 2 13,588 Lancet 30,758 1 30,758 Microb Drug Resist 1,989 2 3,978 Scand J Infect Dis 1,700 1 1,700 Biochemistry 3,226 1 3,226 FEMS Microbiol Lett 2,199 1 2,199 Infection 2,051 1 2,051 Food Microbiology 3,216 1 3,216 Seminars in Hematology 2,703 1 2,703 Eur J Clin Invest 2,643 1 2,643 Microb Pathog 2,324 1 2,324 J Hosp Infect 3,012 1 3,012 J Endocrinol Invest 1,347 1 1,347 Reprod Biol Endocrinol 2,08 1 2,08 96 379,350 Total

List of publications in peer-reviewed journals

1. Tsakris A, Pournaras S, Douboyas J. “Changes in antimicrobial resistance of enterococci isolated in Greece”. J Antimicrob Chemother 1997; 40:735-737. 2. Tsakris A, Woodford N, Pournaras S, Kaufmann M, Douboyas J. “Apparent increased prevalence of high-level aminoglycoside-resistant Enterococcus durans resulting from false identification by a semiautomated software system”. J Clin Microbiol 1998; 36:1419-1421. 3. Skoura L, Efstratiou A, Tsakris A, Pournaras S, George RC, Douboyas J. “Study on the use of an enzyme-linked immunosorbent assay in determining human antibodies to diphtheria toxin as compared with a reference toxin neutralization assay”. Comp Immunol Microbiol Infect Dis 1999; 22:181-186. 4. Tsakris A, Pournaras S, Hathi D, Douboyas J, Efstratiou A. “Outbreak of rare serotype of group A streptococcus pharyngitis in a boarding college”. Lancet 1999; 353:1585-1586. 5. Pournaras S, Tsakris A, Mandraveli K, Faitatzidou A, Douboyas J, Tourkantonis A. “Reported needlestick and sharp injuries among health care workers in a Greek general hospital”. Occup Med 1999; 49:423-426. 6. Pournaras S, Tsakris A, Hadjichristodoulou C, Skoura L, Souliou E, Antoniadis A, Douboyas J. “Diphtheria immunity of Albanian and other eastern European immigrants in Greece compared with the local population. The risk of reemergence in Greece”. Infection 1999; 27:361-364. 7. Tsakris A, Pournaras S, Woodford N, Palepou MFI, Babini GS, Douboyas J, Livermore DM. “Outbreak of infections caused by Pseudomonas aeruginosa producing VIM-1 carbapenemase in Greece”. J Clin Microbiol 2000; 38:1290-1292. 8. Polyzou A, Sofianou D, Pournaras S, Tsakris A. “RAPD-fingerprinting of Serratia marcescens after formaldeyde inactivation of DNAse activity”. Lett Appl Microbiol 2000; 30:419-421. 9. Tsakris A, Pantazi A, Pournaras S, Maniatis A, Polyzou A, Sofianou D. “Pseudo-outbreak of imipenem-resistant Acinetobacter baumannii resulting from false susceptibility testing by a rapid automated system”. J Clin Microbiol 2000; 38:3505-3507. 10. Pournaras S, Efstratiou A, Douboyas J, George RC, Tsakris A. “Genetic relatedness of group A streptococci of the newly designated serotype M90 causing a food-borne outbreak and sporadic infections”. Epidemiol Infect 2000; 125:299-301. 5

11. Pournaras S, Tsakris A, Kaufmann ME, Douboyas J, Antoniadis A. “Outbreak of infections in a Greek university hospital involving a single clone of high-level aminoglycoside-resistant Enterococcus faecalis”. Inf Control Hosp Epidemiol 2000; 21:786-789. 12. Pournaras S, Tsakris A, Palepou MFI, Papa A, Douboyas J, Antoniadis A, Woodford N. “Pheromone responses and high-level aminoglycoside resistance of conjugative plasmids of Enterococcus faecalis from Greece”. J Antimicrob Chemother 2000; 46:1013-1016. 13. Mavroidi A, Tsakris A, Tzelepi E, Pournaras S, Loukova V, Tzouvelekis LS. “Carbapenem- hydrolysing VIM-2 metallo--lactamase in Pseudomonas aeruginosa from Greece”. J Antimicrob Chemother 2000; 46:1041-1043. 14. Pournaras S, Slavakis A, Polyzou A, Sofianou D, Maniatis AN, Tsakris A. “Nosocomial spread of an unusual methicillin-resistant Staphylococcus aureus clone that is sensitive to all non--lactam antibiotics, including tobramycin”. J Clin Microbiol 2001; 39:779-781. 15. Tsakris A, Pournaras S, Maniatis AN, Douboyas J, Antoniadis A. “Increasing prevalence of high-level gentamicin-resistance among enterococci isolated in Greece”. Chemotherapy 2001; 47:86-89. 16. Petinaki E, Miriagou V, Tzouvelekis LS, Pournaras S, Hatzi F, Kontos F, Maniati M, Maniatis AN and the Bacterial resistance Study Group of Thessaly. “Methicillin-resistant Staphylococcus aureus in the hospitals of central Greece”. IntJ Antimicrob Agents 2001; 18:61-65. 17. Polyzou A, Slavakis A, Pournaras S, Maniatis AN, Sofianou D, Tsakris A. “Predominance of a susceptible to erythromycin and several other non--lactam antibiotics methicillin-resistant Staphylococcus aureus clone in a Greek hospital”. J Antimicrob Chemother 2001; 48:231-234. 18. Maniatis AN, Pournaras S, Kanellopoulou M, Kontos F, Dimitroulia E, Papafrangas E, Tsakris A. “Dissemination of clonally unrelated erythromycin- and glycopeptide-resistant Enterococcus faecium isolates in a tertiary greek hospital”. J Clin Microbiol 2001; 39:4571-4574. 19. Kartali G, Tzelepi E, Pournaras S, Kontopoulou C, Kontos F, Sofianou D, Maniatis AN, Tsakris A. “Outbreak of infections caused by Enterobacter cloacae producing the integron-associated β- lactamase IBC-1 in a neonatal intensive care unit of a greek hospital”. Antimicrob Agents Chemother 2002; 46:1577-1580. 20. Tsakris A, Hassapopoulou H, Skoura L, Pournaras S, Douboyas J. “ Leg ulcer due to Pseudomonas luteola in a patient with sickle cell disease.” Diagn Microbiol Infect Dis 2002; 42:141- 143. 6

21. Pournaras S, Tsakris A, Maniati M, Tzouvelekis LS, Maniatis AN. “Novel variant (blaVIM-4) of the metallo-β-lactamase gene blaVIM-1 in a clinical strain of Pseudomonas aeruginosa”. Antimicrob Agents Chemother 2002; 46:4026- 4028. 22. Pournaras S, Maniati M, Petinaki E, Tzouvelekis LS, Tsakris A, Legakis NJ, Maniatis AN. Hospital outbreak of multiple clones of Pseudomonas aeruginosa carrying the unrelated metallo-

{beta}-lactamase gene variants blaVIM-2 and blaVIM-4. J Antimicrob Chemother 2003; 51:1409-1414. 23. Maniatis AN, Pournaras S, Orkopoulou S, Tassios PT, Legakis NJ, and the Bacterial Resistance Study Group. Multi-resistant Acinetobacter baumannii isolates in intensive care units in Greece. Clin Microbiol Infect 2003; 9:547-553. 24. Tsakris A, Tsioni C, Pournaras S, Polyzos S, Maniatis AN, Sofianou D. Spread of low-level carbapenem-resistant Acinetobacter baumannii clones in a tertiary care Greek hospital. J Antimicrob Chemother 2003; 52:1046-1047. 25. Pournaras S, Malamou-Lada H, Maniati M, Mylona-Petropoulou D, Vagiakou-Voudris H, Tsakris A, Maniatis AN. Persistence of a clone of glycopeptide-resistant Enterococcus faecalis among patients in an intensive care unit of a Greek hospital. J Antimicrob Chemother 2004; 53:109-112. 26. Sofianou D, Avgoustinakis E, Dilopoulou A, Pournaras S, Tsirakidis G, Tsakris A. Soft-tissue abscess involving Actinomyces odontolyticus and two Prevotella species in an intravenous drug abuser. Comp Immunol Microbiol Infect Dis 2004; 27:75-79. 27. Kanellopoulou M, Pournaras S, Iglezos H, Papafrangas E, Maniatis AN. Persistent Colonization of Nine Cystic Fibrosis Patients with an Achromobacter (Alcaligenes) xylosoxidans clone. Eur J Clin Microbiol Infect Dis 2004; 23:336-339. 28. Pournaras S, Ikonomidis A, Sofianou D, Tsakris A, Maniatis AN. CTX-M-type beta-lactamases affect community Escherichia coli treatment, Greece. Emerg Infect Dis 2004; 10: 1163-1164. 29. Polyzou A, Pournaras S, Dafni U, Sofianou D, Christeli E, Patrinos S, Tsakris A. Seroepidemiology of Bordetella pertussis immune responses in a healthy population in Northern Greece. J Clin Lab Anal 2004; 18: 211-214. 30. Sofianou D, Pournaras S, Giosi M, Polyzou A, Maniatis AN, Tsakris A. Substantially increased faecal carriage of vancomycin-resistant enterococci in a tertiary Greek hospital after a 4 year time interval. J Antimicrob Chemother 2004; 54: 251-254. 31. Pournaras S, Ikonomidis A, Kristo I, Tsakris A, Maniatis AN. CTX-M enzymes are the most common extended-spectrum beta-lactamases among Escherichia coli in a tertiary Greek hospital. J Antimicrob Chemother 2004; 54: 574-575. 7

32. Pournaras S, Ikonomidis A, Markogiannakis A, Maniatis AN, Tsakris A. Heteroresistance to carbapenems in Acinetobacter baumannii. J Antimicrob Chemother 2005; 55: 1055-1056. 33. Stefos A, Georgiadou SP, Gioti C, Loukopoulos A, Ioannou M, Pournaras S, Dalekos GN. Leptospirosis and pancytopenia: two case reports and review of the literature. J Infect 2005; 51: e277-280. 34. Daponte A, Pournaras S, Zinzaras E, Kallitsaris A, Lialios G, Maniatis AN, Messinis IE. The value of a single combined measurement of VEGF, glycodelin, progesterone, PAPP-A, HPL and LIF for the differentiation between ectopic and abnormal intrauterine pregnancy. Hum Reprod 2005; 20: 3163-3166. 35. Pournaras S, Maniati M, Spanakis N, Ikonomidis A, Tassios PT, Tsakris A, Legakis NJ, Maniatis AN. Spread of efflux pump over-expressing, non-metallo--lactamase-producing, meropenem-resistant but ceftazidime-susceptible Pseudomonas aeruginosa in a region with blaVIM- endemicity. J Antimicrob Chemother 2005; 56: 761-764. 36. Ikonomidis A, Tokatlidou D, Kristo I, Sofianou D, Tsakris A, Mantzana P, Pournaras S, Maniatis AN. Outbreaks in distinct regions due to a single Klebsiella pneumoniae clone carrying blaVIM-1 metallo-b-lactamase gene. J Clin Microbiol 2005; 43: 5344-5347. 37. Karapavlidou P, Sofianou D, Manolis EN, Pournaras S, and Tsakris A. CTX-M-1 extended- spectrum β-lactamase-producing Proteus mirabilis in Greece. Microb Drug Resist 2005; 11: 351- 354. 38. Sofianou D, Markogiannakis A, Metzidie E, Pournaras S, Tsakris A. VIM-2 metallo-β- lactamase in Achromobacter xylosoxidans in Europe. Eur J Clin Microbiol Infect Dis 2005; 24: 854-855. 39. Pournaras S, Ikonomidis A, Tzouvelekis LS, Tokatlidou D, Spanakis N, Maniatis AN, Legakis NJ, Tsakris A. VIM-12, a novel plasmid-mediated metallo-{beta}-lactamase from Klebsiella pneumoniae that resembles a VIM-1/VIM-2 hybrid. Antimicrob Agents Chemother 2005; 49: 5153-5156. 40. Metzidie E, Manolis EN, Pournaras S, Sofianou D, Tsakris A. Spread of an unusual penicillin- and imipenem-resistant but ampicillin-susceptible phenotype among Enterococcus faecalis clinical isolates. J Antimicrob Chemother 2006; 57: 158-160. 41. Pournaras S, Markogiannakis A, Ikonomidis A, Kondyli L, Bethimouti K, Maniatis AN, Legakis NJ, Tsakris A. Outbreak of multiple clones of imipenem-resistant Acinetobacter baumannii isolates expressing OXA-58 carbapenemase in an intensive care unit. J Antimicrob Chemother 2006; 57: 557-561. 8

42. Pournaras S, Tsakris A, Ikonomidis A. Markogiannakis A, Kristo I, Maniatis AN. Detection of a novel variant blaCTX-M-3 extended spectrum β-lactamase gene in a community-acquired Escherichia coli isolate. Scand J Infect Dis 2006; 38: 213-216. 43. Daponte A, Pournaras S, Hadjichristodoulou C, Lialios G, Kallitsaris A, Maniatis AN, Messinis IE. Novel serum inflammatory markers in patients with adnexal mass who were operated on because of ovarian torsion. Fertil Steril 2006; 85: 1469-1472. 44. Daponte A, Pournaras S, Mademtzis I, Hadjichristodoulou C, Kostopoulou E, Maniatis AN, Messinis IE. Evaluation of HPV 16 PCR detection in self- compared with clinician-collected samples in women referred for colposcopy. Gynecol Oncol 2006; 103: 463-466. 45. Daponte A, Pournaras S, Mademtzis I, Hadjichristodoulou C, Kostopoulou E, Maniatis AN, Messinis IE. Evaluation of high-risk human papillomavirus types PCR detection in paired urine and cervical samples of women with abnormal cytology. J Clin Virol 2006; 36: 189-193. 46. Tsakris A, Ikonomidis A, Pournaras S, Tzouvelekis LS, Sofianou D, Legakis NJ, Maniatis AN. VIM-1 Metallo-β-lactamase in Acinetobacter baumannii. Emerg Infect Dis 2006; 12: 981-83. 47. Ikonomidis A, Pournaras S, Maniatis AN, Legakis NJ, Tsakris A. Discordance of meropenem versus imipenem activity aganist Acinetobacter baumannii. Int J Antimicrob Agents 2006; 28: 376-377 48. Tsakris A, Ikonomidis A, Pournaras S, Spanakis N, Markogiannakis A. Carriage of OXA-58 but not of OXA-51 correlates with carbapenem resistance in Acinetobacter baumannii. J Antimicrob Chemother 2006; 58: 1097-1099. 49. Pournaras S, Ikonomidis A, Markogiannakis A, Spanakis N, Maniatis AN, Tsakris A. “Characterisation of clinical isolates of Pseudomonas aeruginosa heterogeneously resistant to carbapenems”. J Med Microbiol. 2007; 56: 66-70 50. Tsakris A, Ikonomidis A, Spanakis N, Pournaras S, Bethimouti K. “Identification of a novel blaOXA-51 variant, blaOXA-92, from a clinical isolate of Acinetobacter baumannii”. Clin Microbiol Infect 2007; 13: 348-349. 51. Ikonomidis A, Pournaras S, Markogiannakis A, Maniatis AN, Manolis EN, Tsakris A. “Low genetic diversity of the intrinsic OXA-51-like class D carbapenemases among Acinetobacter baumannii clinical isolates in Greece”. Epidemiol Infect 2007; 135: 877-881. 52. Tsakris A, Ikonomidis A, Spanakis N, Poulou A, Pournaras S. “Characterization of In3Mor, a new integron carrying VIM-1 metallo-{beta}-lactamase and sat1 gene, from Morganella morganii.” J Antimicrob Chemother 2007; 59: 739-41. 53. Tsakris A, Ikonomidis A, Poulou A, Spanakis N, Pournaras S, Markou F. “Transmission in the community of clonal Proteus mirabilis carrying VIM-1 metallo-beta- lactamase”. J Antimicrob Chemother 2007; 60: 136-39.

54. Maniati M, Ikonomidis A, Mantzana P, Daponte A, Maniatis AN, Pournaras S. “A highly carbapenem resistant Pseudomonas aeruginosa isolate with a novel blaVIM-4/blaP1b integron over-expresses two efflux pumps and lacks OprD”. J Antimicrob Chemother 2007; 60: 132-5. 55. Ikonomidis A, Labrou M, Afkou Z, Sofianou D, Maniatis AN, Tsakris A, Pournaras S. “First Occurrence of an Escherichia coli Clinical Isolate Producing the VIM-1/VIM-2 Hybrid Metallo-β- Lactamase VIM-12”. Antimicrob Agents Chemother 2007; 51: 3038-9. 56. Meletiadis J, Antachopoulos C, Stergiopoulou T, Pournaras S, Roilides E, Walsh TJ. “Differential fungicidal activity of amphotericin B and voriconazole against Aspergillus species determined by microbroth methodology”. Antimicrob Agents Chemother 2007; 51: 3329-3337. 57. Konstantinidis A, Minas A, Pournaras S, Kansouzidou A, Papastergiou P, Maniatis A, Stathakis N, Hadjichristodoulou C. “Evaluation and comparison of fluorescence polarization assay with three of the currently used serological tests in diagnosis of human brucellosis”. Eur J Clin Microbiol Infect Dis. 2007; 26: 715-721. 58. Kontou Μ, Pournaras S, Kristo I, Ikonomidis A, Maniatis AN, Stathopoulos C. Molecular cloning and biochemical characterization of VIM-12, a novel hybrid VIM-1/VIM-2 metallo-beta- lactamase from a Klebsiella pneumoniae clinical isolate, reveals atypical substrate specificity. Biochemistry. 2007; 46: 13170-13178. 59. Ikonomidis A, Tsakris A, Kantzanou M, Spanakis N, Maniatis AN, Pournaras S. Efflux systems overexpression and decreased OprD contribute to the carbapenem heterogeneous growth in Pseudomonas aeruginosa. FEMS Microbiol Lett. 2008; 279: 36-39. 60. Ikonomidis A, Spanakis N, Poulou A, Pournaras S, Markou F, Tsakris A. Emergence of carbapenem-Resistant Enterobacter cloacae Carrying VIM-4 Metallo-β-Lactamase and SHV-2a Extended-Spectrum β-Lactamase in a Congugative Plasmid. Microb Drug Resist. 2007; 13: 221- 226. 61. Ikonomidis A, Ntokou E, Maniatis AN, Tsakris A, Pournaras S. “Hidden VIM-1 metallo-beta- lactamase phenotypes among Acinetobacter baumannii clinical isolates”. J Clin Microbiol 2008; 46: 346-349. 62. Tokatlidou D, Tsivitanidou M, Pournaras S, Ikonomidis A, Tsakris A, Sofianou D. *Outbreak

Caused by a Multidrug-Resistant Klebsiella pneumoniae Clone Carrying blaVIM-12 in a University Hospital. J Clin Microbiol 2008; 46: 1005-1008. 63. Pournaras S, Ikonomidis A, Neou E, Kantzanou M, Maniatis AN, Tsakris A. Piperacillin/tazobactam-heteroresistant Pseudomonas aeruginosa from urinary infection, successfully treated by piperacillin/tazobactam. J Antimicrob Chemother 2008; 61: 757-758. 10

64. Daponte A, Tsezou A, Oikonomou P, Hadjichristodoulou C, Maniatis A, Pournaras S, Messinis IE. Use of real-time PCR to detect human papillomavirus-16 viral loads in vaginal and urine self-sampled specimens. Clin Microbiol Infect. 2008; 14: 619-621. 65. Chalvatzas N, Dafopoulos K, Kosmas G, Kallitsaris A, Pournaras S, Messinis IE. Effect of ovarian hormones on serum adiponectin and resistin concentrations. Fertil Steril. 2008 Mar 4. [Epub ahead of print]. 66. Markogiannakis A, Fildisis G, Tsiplakou S, Ikonomidis A, Koutsoukou A, Pournaras S, Manolis EN, Baltopoulos G, Tsakris A. Cross-Transmission of Multidrug-Resistant Acinetobacter baumannii Clonal Strains Causing Episodes of Sepsis in a Trauma Intensive Care Unit. Infect. Control Hosp. Epidemiol. 2008; 29: 410-417. 67. Tsakris A, Ikonomidis A, Poulou A, Spanakis N, Vrizas D, Diomidous M, Pournaras S, Markou F. Clusters of imipenem-resistant Acinetobacter baumannii clones producing different carbapenemases in an intensive care unit. Clin Microbiol Infect. 2008; 14: 588-594. 68. Vasilaki O, Ntokou E, Ikonomidis A, Sofianou D, Frantzidou F, Alexiou-Daniel S, Maniatis AN, Pournaras S. Emergence of the plasmid-mediated quinolone resistance gene qnrS1 in Escherichia coli genotypes in Greece. Antimicrob Agents Chemother. 2008; 52:2996-2997. 69. Ikonomidis A, Michail G, Vasdeki A, Labrou M, Karavasilis V, Stathopoulos C, Maniatis AN, Pournaras S. In Vitro and In Vivo Evaluation of Oxacillin Efficiency on mecA-Positive Oxacillin- Susceptible Staphylococcus aureus. Antimicrob Agents Chemother. 2008: 52: 3905-3908. 70. Tsakris A, Kristo I, Poulou A, Markou F, Ikonomidis A, Pournaras S. First occurrence of KPC- 2-possessing Klebsiella pneumoniae in a Greek hospital and recommendation for detection with boronic acid disc tests. J Antimicrob Chemother. 2008: 62: 1257-1260. 71. Ikonomidis A, Tsakris A, Kanellopoulou M, Maniatis AN, Pournaras S. Effect of the proton motive force inhibitor carbonyl cyanide-m-chlorophenylhydrazone (CCCP) on Pseudomonas aeruginosa biofilm development. Lett Appl Microbiol. 2008: 47: 298-302. 72. Dafopoulos K, Sourlas D, Kallitsaris A, Pournaras S, Messinis IΕ. Blood ghrelin, resistin and adiponectin concentrations during the normal menstrual cycle. Fertil Steril 2009: 92: 1389-1394. 73. Tsakris A, Kristo I, Poulou A, Themeli-Digalaki K, Ikonomidis A, Petropoulou D, Pournaras S, Sofianou D. Evaluation of boronic acid disk tests for differentiating KPC-possessing Klebsiella pneumoniae isolates in the clinical laboratory. J Clin Microbiol 2009: 47: 362-367 74. Dafopoulos K, Venetis C, Pournaras S, Kallitsaris A, Messinis IΕ. Ovarian control of pituitary sensitivity of luteinizing hormone secretion to gonadotropin-releasing hormone in women with the polycystic ovary syndrome. Fertil Steril 2009: 92: 1378-1380 11

75. Pournaras S, Iosifidis E, Roilides E. Advances in antibacterial therapy against emerging bacterial pathogens. Seminars in Hematology 2009; in press 76. Pournaras S, Protonotariou E, Voulgari E, Kristo I, Dimitroulia E, Vitti D, Tsalidou M, Maniatis AΝ, Tsakris A, Sofianou D. Clonal spread of KPC-2 carbapenemase-producing Klebsiella pneumoniae strains in Greece. J Antimicrob Chemother 2009; in press 77. Solomakos Ν, Govaris Α, Angelidis As, Pournaras S, Rothi Burriel A, Kritas Sk And Papageorgiou Dk. Occurrence, virulence genes and antibiotic resistance of Escherichia coli O157 isolated from raw bovine, caprine and ovine milk in Greece. Food Microbiol. 2009 Dec;26(8):865- 71. 78. Ikonomidis A, Neou E, Gogou V, Vrioni G, Tsakris A, Pournaras S. Heteroresistance to meropenem in carbapenem-susceptible Acinetobacter baumannii. J Clin Microbiol. 2009; 47: 4055-9.

79. Ikonomidis A, Vasdeki A, Kristo I, Maniatis AN, Tsakris A, Malizos KN, Pournaras S. Association of biofilm formation and methicillin-resistance with accessory gene regulator (agr) loci in Greek Staphylococcus aureus clones. Microb Pathog. 2009; 47: 341-344. 80. Tsakris A, Poulou A, Themeli-Digalaki K, Voulgari E, Pittaras T, Sofianou D, Pournaras S, Petropoulou D. Use of boronic acid disk tests to detect extended- spectrum beta-lactamases in clinical isolates of KPC carbapenemase-possessing enterobacteriaceae. J Clin Microbiol. 2009; 47: 3420-3426. 81. Tsakris A, Poulou A, Kristo I, Pittaras T, Spanakis N, Pournaras S, Markou F. Large dissemination of VIM-2-metallo-beta-lactamase-producing pseudomonas aeruginosa strains causing health care-associated community-onset infections. J Clin Microbiol. 2009; 47: 3524-3529. 82. Papaioannou AI, Koutsokera A, Tanou K, Kiropoulos TS, Tsilioni I, Oikonomidi S, Liadaki K, Pournaras S, Gourgoulianis KI, Kostikas K. The acute effect of smoking in healthy and asthmatic smokers. Eur J Clin Invest. 2010: 40: 102-109. 83. Meletiadis J, Pournaras S, Roilides E, Walsh TJ. Defining Fractional Inhibitory Concentration Index Cutoffs for additive Interactions based on Self-Drug Additive Combinations, Monte Carlo Simulation Analysis and in vitro-in vivo Correlation Data for Antifungal Drug Combinations against Aspergillus fumigatus. Antimicrob. Agents Chemother. 2010: 54: 602-609.

84. Miriagou V, Cornaglia G, Edelstein M, Galani I, Giske Cg, Gniadkowski M, Malamou-Lada E, Martinez-Martinez L, Navarro F, Nordmann P, Peixe L, Pournaras S, Rossolini GM, Tsakris A, Vatopoulos A, Cantón R. Acquired carbapenemases in Gram-negative bacterial pathogens: detection and surveillance issues. Clin Microbiol Infect 2010; 16:112-122. 12

85. Giannouli S, Labrou M, Kyritsis A, Ikonomidis A, Pournaras S, Stathopoulos C, Tsakris A. Detection of mutations in the FemXAB protein family in oxacillin-susceptible mecA-positive Staphylococcus aureus clinical isolates. J Antimicrob Chemother. 2010; 65: 626-33. 86. Dafopoulos K, Chalvatzas N, Kosmas G, Kallitsaris A, Pournaras S, Messinis IE . The effect of estrogens on plasma ghrelin concentrations in women. J Endocrinol Invest. 2010; 33:109-12 87. Zarkotou O, Pournaras S, Voulgari E, Chrysos G, Prekates A, Voutsinas D, Themeli-Digalaki K, Tsakris A. Risk Factors and Outcomes Associated with Acquisition of Colistin-Resistant KPC- Producing Klebsiella pneumoniae: a Matched Case-Control Study. J Clin Microbiol. 2010: 48: 2271-2274. 88. Protonotariou E, Dimitroulia E, Pournaras S, Pitiriga V, Sofianou D, Tsakris A. Trends in antimicrobial resistance of clinical isolates of Enterococcus faecalis and Enterococcus faecium in Greece between 2002 and 2007. J Hosp Infect. 2010; 75: 225-227. 89. Pournaras S, Poulou A, Tsakris A. Inhibitor-based methods for the detection of KPC carbapenemase-producing Enterobacteriaceae in clinical practice by using boronic acid compounds. J Antimicrob Chemother. 2010; 65: 1319-1321.

90. Tsakris A, Voulgari E, Poulou A, Kimouli M, Pournaras S, Ranellou K, Kosmopoulou O,

Petropoulou D. In vivo acquisition of a plasmid-mediated blaKPC-2 gene among clonal isolates of Serratia marcescens. J Clin Microbiol. 2010 2010; 48: 2546-2549.

91. Pournaras S, Kristo I, Vrioni G, Ikonomidis A, Poulou A, Petropoulou D, Tsakris A. Characteristics of meropenem heteroresistance in KPC carbapenemase-producing Klebsiella pneumoniae. J Clin Microbiol. 2010; 48: 2601-2604.

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