Natural England Commissioned Report NECR258 An assessment of the fungal conservation value of Hardcastle Crags (Hebden Bridge, West Yorkshire) using NextGen DNA sequencing of soil samples First published February 2019 Month XXXX www.gov.uk/natural-england Foreword Natural England commission a range of reports from external contractors to provide evidence and advice to assist us in delivering our duties. The views in this report are those of the authors and do not necessarily represent those of Natural England. Background DNA based applications have the potential to significantly change how we monitor and assess This report should be cited as: biodiversity. These techniques may provide cheaper alternatives to existing species GRIFFITHS, G.W., CAVALLI, O. & monitoring or an ability to detect species that we DETHERIDGE, A.P. 2019. An assessment of the cannot currently detect reliably. fungal conservation value of Hardcastle Crags (Hebden Bridge, West Yorkshire) using NextGen Natural England has been supporting the DNA sequencing of soil samples. Natural development of DNA techniques for a number of England Commissioned Reports, Number 258. years and has funded exploratory projects looking at different taxonomic groups in a range of different ecosystems and habitats. This report presents the results of a survey of fungi of conservation importance at Hardcastle . Crags, West Yorkshire, using DNA based methods. These results are compared to fruiting body surveys which had been carried out in the preceding two years. Natural England Project Manager – Andy Nisbet, Principal Adviser and Evidence Programme Manager, Natural England
[email protected] Contractor – Gareth Griffith, Aberystwyth University
[email protected] Keywords – DNA, eDNA, sequencing, metabarcoding, monitoring, fungi, waxcaps, grassland Further information This report can be downloaded from the Natural England Access to Evidence Catalogue: http://publications.naturalengland.org.uk/ .