EIF4E Antibody Cat. No.: 60-600
EIF4E Antibody
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4E antibody (N-term), which was peroxidase- conjugated to the secondary antibody, followed by DAB staining.
Specifications
HOST SPECIES: Rabbit
SPECIES REACTIVITY: Human
Predicted species reactivity based on immunogen sequence: Zebrafish, Bovine, Mouse, HOMOLOGY: Rabbit, Rat, Xenopus
This EIF4E antibody is generated from rabbits immunized with a KLH conjugated synthetic IMMUNOGEN: peptide between 32-61 amino acids from the N-terminal region of human EIF4E.
TESTED APPLICATIONS: IHC-P, WB
For WB starting dilution is: 1:1000 APPLICATIONS: For IHC-P starting dilution is: 1:50~100
September 27, 2021 1 https://www.prosci-inc.com/eif4e-antibody-60-600.html PREDICTED MOLECULAR 25 kDa WEIGHT:
Properties
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by PURIFICATION: dialysis
CLONALITY: Polyclonal
ISOTYPE: Rabbit Ig
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
CONCENTRATION: batch dependent
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
OFFICIAL SYMBOL: EIF4E
Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap- ALTERNATE NAMES: binding protein, EIF4E, EIF4EL1, EIF4F
ACCESSION NO.: P06730
PROTEIN GI NO.: 1352435
GENE ID: 1977
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 BACKGROUND: and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP- kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. this gene also interacts mutually exclusive with EIF4A1 and EIF4A2.
REFERENCES: 1) Rychlik,W., J. Biol. Chem. 262 (22), 10434-10437 (1987)
September 27, 2021 2 https://www.prosci-inc.com/eif4e-antibody-60-600.html 2) Dorfman,J., Genomics(4), 785-788 (1991)
3) Pelletier,J., Genomics 10 (4), 1079-1082 (1991)
4) Whalen,S.G., J. Biol. Chem. 271 (20), 11831-11837 (1996)
ANTIBODIES FOR RESEARCH USE ONLY.
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September 27, 2021 3 https://www.prosci-inc.com/eif4e-antibody-60-600.html