DOCSLIB.ORG

A Synopsis of the Genera and Species in the Tylenchorhynchinae (Tylenchoidea, Nematoda)1

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
A Synopsis of the Genera and Species in the Tylenchorhynchinae (Tylenchoidea, Nematoda)1 OF WASHINGTON, VOLUME 40, NUMBER 1, JANUARY 1973 123 Speer, C. A., and D. M. Hammond. 1970. tured bovine cells. J. Protozool. 18 (Suppl.): Development of Eimeria larimerensis from the 11. Uinta ground squirrel in cell cultures. Ztschr. Vetterling, J. M., P. A. Madden, and N. S. Parasitenk. 35: 105-118. Dittemore. 1971. Scanning electron mi- , L. R. Davis, and D. M. Hammond. croscopy of poultry coccidia after in vitro 1971. Cinemicrographic observations on the excystation and penetration of cultured cells. development of Eimeria larimerensis in cul- Ztschr. Parasitenk. 37: 136-147. A Synopsis of the Genera and Species in the Tylenchorhynchinae (Tylenchoidea, Nematoda)1 A. C. TARJAN2 ABSTRACT: The genera Uliginotylenchus Siddiqi, 1971, Quinisulcius Siddiqi, 1971, Merlinius Siddiqi, 1970, Ttjlenchorhynchus Cobb, 1913, Tetylenchus Filipjev, 1936, Nagelus Thome and Malek, 1968, and Geocenamus Thorne and Malek, 1968 are discussed. Keys and diagnostic data are presented. The following new combinations are made: Tetylenchus aduncus (de Guiran, 1967), Merlinius al- boranensis (Tobar-Jimenez, 1970), Geocenamus arcticus (Mulvey, 1969), Merlinius brachycephalus (Litvinova, 1946), Merlinius gaudialis (Izatullaeva, 1967), Geocenamus longus (Wu, 1969), Merlinius parobscurus ( Mulvey, 1969), Merlinius polonicus (Szczygiel, 1970), Merlinius sobolevi (Mukhina, 1970), and Merlinius tatrensis (Sabova, 1967). Tylenchorhynchus galeatus Litvinova, 1946 is with- drawn from the genus Merlinius. The following synonymies are made: Merlinius berberidis (Sethi and Swarup, 1968) is synonymized to M. hexagrammus (Sturhan, 1966); Ttjlenchorhynchus chonai Sethi and Swarup, 1968 is synonymized to T. triglyphus Seinhorst, 1963; Quinisulcius nilgiriensis (Seshadri et al., 1967) is synonymized to Q. acti (Hopper, 1959); and Tylenchorhynchus tener Erzhanova, 1964 is regarded a synonym of T. clarus Allen, 1955. The following are regarded in spe- cies inquirendae: TetylencJius dimidius Kirjanova, 1951; Aphelenchus dubius Steiner, 1914; and Fratylenchoides gadeai Arias-D, Jimenez-M. and Lopez-P., 1965. Since my first compendium on Ttjlencho- proposed the new subfamily Merliniinae to ac- rhynchus (Tarjan, 1964), there have been a commodate Merlinius and formed two new number of new species proposed, two addi- genera within the Tylenchorhynchinae made tional keys prepared (Baqri and Jairajpuri, up of certain species formerly in Tylenchorhyn- 1970 and de Guiran, 1967), and new genera chus. The first of these is Uliginotylenchus proposed which are closely related to Tylen- with three incisures comprising an areolated chorhynchus (Siddiqi, 1970, 1971; Thorne and lateral field, female tail clavate to cylindroid Malek, 1968). In his first paper Siddiqi and with over 25 annules, and proximal end (1970) erected the new subfamily Tetylen- of gubernaculum bent dorsally. The second chinae to include the genus Tetylenchus and genus, Quinisulcius, has a nonareolated lateral proposed a new genus Merlinius to accommo- field with five incisures, distal flanges of date species of Tylenchorhynchus having six spicula are small-sized, and the proximal end incisures in the lateral field, a small trough- shaped nonprotrusible gubernaculum, and stout of gubernaculum directed dorsally. spicules with distal ends notched and without It is the object of the present study to large ventral flanges. Siddiqi's second paper evaluate the position of Tijlenchorhynchus and closely related genera, to determine member- 1 Florida Agricultural Experiment Stations Journal Series ship in such genera, and to present keys and a No. 4450. -Professor (Nematologist), University of Florida, IF AS, table of diagnostic data on species. The work Agricultural Research and Education Center, Lake Alfred, Florida 33850. presented was compiled only from publica- Copyright © 2011, The Helminthological Society of Washington 124 PROCEEDINGS OF THE HELMINTHOLOGICAL SOCIETY tions and not from actual examination of present status of Trichotylenchus Whitehead, specimens. 1959 and Telotylenchus Siddiqi, 1960. Jairajpuri (1969) stated that Trichotylenchus differed Subfamily considerations from Telotylenchus only by a slightly narrower The Tylenchorhynchinae are, and have been, head, delicate spear, and three-incisured lateral a relatively homogenous group readily recog- field. In a later paper (1971), he presented nized by their cuticular markings, labial struc- a more detailed account corroborating and ture, stylet characteristics, esophagus structure, illustrating these differences. Jairajpuri re- sexual system arrangement, and tail shape. I garded Telotylenchus a junior synonym of see no practical value in assigning such closely Trichotylenchus. Seinhorst (1971) did not related genera as Tetylenchus, Tylenchorhyn- refer to the Jairajpuri references and regarded chus, and Merlinius to three different sub- the two genera as distinct, separated by stylet families, e.g., Tetylenchinae, Tylenchorhyn- appearance, shape of female tail, lateral field, chinae, and Merliniinae, respectively (Siddiqi, and shape of gubernaculum. He included 1971). Accordingly, I prefer to follow the ar- Trichotylenchus falciformis Whitehead, 1959; rangement suggested by Golden (1971) in T. rectangularis Netscher and Germani, 1969; recognizing one subfamily, the Tylenchorhyn- T. rhopalocercus (Seinhorst, 1963); T. palustris chinae, in which the new genus Merlinius (Merny and Germani, 1968); T. uliginosus (Siddiqi, 1970) can also be conveniently (Siddiqi, 1970); and T. papyrus (Siddiqi, accommodated. 1970) in the genus. He justified inclusion of some of these species, which in their original Previous departures descriptions do not all have the same type of basal portion of the esophagus, by suggesting In addition to the accurate list given by that the original authors misinterpreted the Baker (1962), Table 2 cites departures from true structure of the esophagus. Seinhorst's Tylenchorhynchus as given by various authors placement of these species thus results in a during the past two decades. genus in which: (a) two species (falciformis and rectangularis) have pronounced esopha- The genus Uliginotylenchus Siddiqi, 1971 geal overlaps, while four species do not; (b) This genus was characterized essentially as five species have delicate or slender stylets having an areolated lateral field with three in- while one (palustris) does not; (c) five species cisures, moderately sclerotized cephalic frame- have three-incisured lateral fields while one work, labial region not offset, spermatheca (rectangularis) has four incisures; and (d) offset, spicules with large-sized distal flanges, four species have somewhat clavate tails while gubernaculum with proximal portion directed two species (rectangularis and palustris) have dorsally, female tail with 25 or more annules cylindrical tails. Then, too, no mention is and broadly rounded, and occurring in wet made of Whitehead's (1959) original char- soil. The characters above appear to be valid acterization of Trichotylenchus as having a except for the cephalic framework which is head divided into four lobes and a distinctly invariably lightly, not moderately, sclerotized forked stylet. Because of these discrepancies for the five species included and for the offset and because I feel that the distinct and pro- nature of the spermatheca which is unknown nounced overlap of the esophagus on the in- for four of the five species. Of the species testine is of cardinal importance in identifying included, U. bifasciatus (Andrassy, 1961) ap- species of Trichotylenchus, I regard Siddiqi's pears to deviate most from the generic criteria (1971) assignment of species as the most in that: (a) its lip region is weakly offset, not logical. continuous; (b) its males do not show spicules It would be convenient to include the four with large-sized distal flanges; and (c) its remaining three-incisured "Tylenchorhynchus" type locality was in garden soil, not marshy or species in Uliginotylenchus, i.e., T. chonai, T. wet soil. divittatus, T. sculptus, and T. triglyphus. This Uliginotylenchus cannot be evaluated with- is not feasible principally because: (a) none out referring to the current controversy on the of the species have areolated lateral fields, Copyright © 2011, The Helminthological Society of Washington OF WASHINGTON, VOLUME 40, NUMBER 1, JANUARY 1973 125 broadly rounded female tails with more than The generic diagnosis given by Golden (1971), 25 annules, or occur in wet soils, and (b) most patterned after previous diagnoses, is more of these species do not have gubernacula with inclusive. However, one of the principal char- proximal parts directed dorsally, spicules with acters of the genus, "cephalic framework large-sized distal flange, or lightly sclerotized present," becomes suspect when diagnostic cephalic frameworks. data on Tylenchorhynchus species is inspected (Table 1). Under the heading of "framework Key to Females of Uliginotylenchus* sclerotization" it can be seen that among 45 1. Tail with 24-35 annules, lip region with species, 13 have inconspicuous cephalic frame- 4-5 annules, tail terminus smooth ____ works, 6 have frameworks which were not ____ palustris (Merny and Germani, 1968) mentioned or depicted, while 15 have lightly Siddiqi, 1971 sclerotized frameworks. Tail with 42-56 annules, lip region with Siddiqi (1970) transferred Tylenchorynchus 5—8 annules, tail terminus annulated 2 galeatus Litvinova, 1946 to Merlinius. He later 2. T/ABW = 2.6-2.7, lip region 5-6 an- agreed (in litt.) that the species best be taken
Load more

Recommended publications
  • One New and Three Known Species of the Genus Helicotylenchus Steiner, 1945 Associated with Banana from West Bengal, India
    Rec. zool. Surv. India: 110(Part-3) : 7-12, 2010 ONE NEW AND THREE KNOWN SPECIES OF THE GENUS HELICOTYLENCHUS STEINER, 1945 ASSOCIATED WITH BANANA FROM WEST BENGAL, INDIA VISWA VENKAT GANTAITl *, TANMAY BHATTACHARYA2 AND AMALENDU CHATTERJEEl INemathelminthes Section, Zoological Survey of India, M-Block, New Alipur, Kolkata-700 053, West Bengal, India. 2Department of Zoology, Vzdyasagar University, Medinipur, 721102, Paschim Medinipur, West Bengal, India. * Corresponding author: [email protected] INTRODUCTION slow dehydration method (Seinhorst, 1959); mounted One new and three known species of plant parasitic on slides in anhydrous glycerin and sealed. nematodes belonging to the genus Helicotylenchus Measurements were taken with the help of an ocular Steiner, 1945, H. wasimi n. sp., H. crenacauda Sher, micrometer using BX 41 Olympus research microscope 1966, H. dihystera (Cobb, 1893) Sher, 1961 and H. with drawing tube attachment. Dimensions were hydrophilus Sher, 1966 are being described and tabulated in accordance with De Man's Formula (De illustrated. The species were collected from rhizospheric Man, 1884). Diagrams were drawn with the help of a soil of banana plantations (Musa paradisiaca L. cv camera lucida. Kanthali) in Paschim Medinipur district of West Bengal, Abbreviations used in the text as well as in the India, during a taxonomic survey from March 2004 to table are as follows : February 2006. H. wasimi n. sp. is characterized by its L body length rounded lip region without annulations, 26-27 ]lm long a body length / maximum body width spear with anteriorly indented knobs, posteriorly located b body length / oesophageal length (808-809 ]lm from head end) vulva, anterior genital c body length / tail length branch longer than posterior one, short hemispherical cl tail length / body width at anus tail with thick cuticular rounded terminus, tail with four v distance from head end to vulva x 100 / distinct striations and phasmids located opposite to body length anus.
    [Show full text]
  • Population Variability of Rotylenchulus Reniformis in Cotton Agroecosystems Megan Leach Clemson University, [email protected]
    Clemson University TigerPrints All Dissertations Dissertations 12-2010 Population Variability of Rotylenchulus reniformis in Cotton Agroecosystems Megan Leach Clemson University, [email protected] Follow this and additional works at: https://tigerprints.clemson.edu/all_dissertations Part of the Plant Pathology Commons Recommended Citation Leach, Megan, "Population Variability of Rotylenchulus reniformis in Cotton Agroecosystems" (2010). All Dissertations. 669. https://tigerprints.clemson.edu/all_dissertations/669 This Dissertation is brought to you for free and open access by the Dissertations at TigerPrints. It has been accepted for inclusion in All Dissertations by an authorized administrator of TigerPrints. For more information, please contact [email protected]. POPULATION VARIABILITY OF ROTYLENCHULUS RENIFORMIS IN COTTON AGROECOSYSTEMS A Dissertation Presented to the Graduate School of Clemson University In Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy Plant and Environmental Sciences by Megan Marie Leach December 2010 Accepted by: Dr. Paula Agudelo, Committee Chair Dr. Halina Knap Dr. John Mueller Dr. Amy Lawton-Rauh Dr. Emerson Shipe i ABSTRACT Rotylenchulus reniformis, reniform nematode, is a highly variable species and an economically important pest in many cotton fields across the southeast. Rotation to resistant or poor host crops is a prescribed method for management of reniform nematode. An increase in the incidence and prevalence of the nematode in the United States has been reported over the
    [Show full text]
  • Investigation of the Development of Root Lesion Nematodes, Pratylenchus Spp
    Türk. entomol. derg., 2021, 45 (1): 23-31 ISSN 1010-6960 DOI: http://dx.doi.org/10.16970/entoted.753614 E-ISSN 2536-491X Original article (Orijinal araştırma) Investigation of the development of root lesion nematodes, Pratylenchus spp. (Tylenchida: Pratylenchidae) in three chickpea cultivars Kök lezyon nematodlarının, Pratylenchus spp. (Tylenchida: Pratylenchidae) üç nohut çeşidinde gelişmesinin incelenmesi İrem AYAZ1 Ece B. KASAPOĞLU ULUDAMAR1* Tohid BEHMAND1 İbrahim Halil ELEKCİOĞLU1 Abstract In this study, penetration, population changes and reproduction rates of root lesion nematodes, Pratylenchus neglectus (Rensch, 1924), Pratylenchus penetrans (Cobb, 1917) and Pratylenchus thornei Sher & Allen, 1953 (Tylenchida: Pratylenchidae), at 3, 7, 14, 21, 28, 35, 42, 49 and 56 d after inoculation in chickpea Bari 2, Bari 3 (Cicer reticulatum Ladiz) and Cermi [Cicer echinospermum P.H.Davis (Fabales: Fabaceae)] were assessed in a controlled environment room in 2018-2019. No juveniles were observed in the roots in the first 3 d after inoculation. Although, population density of P. thornei reached the highest in Cermi (21 d), Bari 3 (42 d) and the lowest observed on Bari 2. Pratylenchus neglectus reached the highest population density in Bari 3 and Cermi on day 28. The population density of P. neglectus was the lowest in Bari 2. Also, population density of P. penetrans reached the highest in Bari 3 cultivar within 49 d, similar to P. thornei, whereas Bari 2 and Cermi had low population densities during the entire experimental period. Keywords:
    [Show full text]
  • Occurrence of Ditylenchus Destructorthorne, 1945 on a Sand
    Journal of Plant Protection Research ISSN 1427-4345 ORIGINAL ARTICLE Occurrence of Ditylenchus destructor Thorne, 1945 on a sand dune of the Baltic Sea Renata Dobosz1*, Katarzyna Rybarczyk-Mydłowska2, Grażyna Winiszewska2 1 Entomology and Animal Pests, Institute of Plant Protection – National Research Institute, Poznan, Poland 2 Nematological Diagnostic and Training Centre, Museum and Institute of Zoology Polish Academy of Sciences, Warsaw, Poland Vol. 60, No. 1: 31–40, 2020 Abstract DOI: 10.24425/jppr.2020.132206 Ditylenchus destructor is a serious pest of numerous economically important plants world- wide. The population of this nematode species was isolated from the root zone of Ammo- Received: July 11, 2019 phila arenaria on a Baltic Sea sand dune. This population’s morphological and morphomet- Accepted: September 27, 2019 rical characteristics corresponded to D. destructor data provided so far, except for the stylet knobs’ height (2.1–2.9 vs 1.3–1.8) and their arrangement (laterally vs slightly posteriorly *Corresponding address: sloping), the length of a hyaline part on the tail end (0.8–1.8 vs 1–2.9), the pharyngeal gland [email protected] arrangement in relation to the intestine (dorsal or ventral vs dorsal, ventral or lateral) and the appearance of vulval lips (smooth vs annulated). Ribosomal DNA sequence analysis confirmed the identity of D. destructor from a coastal dune. Keywords: Ammophila arenaria, internal transcribed spacer (ITS), potato rot nematode, 18S, 28S rDNA Introduction Nematodes from the genus Ditylenchus Filipjev, 1936, arachis Zhang et al., 2014, both of which are pests of are found in soil, in the root zone of arable and wild- peanut (Arachis hypogaea L.), Ditylenchus destruc- -growing plants, and occasionally in the tissues of un- tor Thorne, 1945 which feeds on potato (Solanum tu- derground or aboveground parts (Brzeski 1998).
    [Show full text]
  • The Complete Mitochondrial Genome of the Columbia Lance Nematode
    Ma et al. Parasites Vectors (2020) 13:321 https://doi.org/10.1186/s13071-020-04187-y Parasites & Vectors RESEARCH Open Access The complete mitochondrial genome of the Columbia lance nematode, Hoplolaimus columbus, a major agricultural pathogen in North America Xinyuan Ma1, Paula Agudelo1, Vincent P. Richards2 and J. Antonio Baeza2,3,4* Abstract Background: The plant-parasitic nematode Hoplolaimus columbus is a pathogen that uses a wide range of hosts and causes substantial yield loss in agricultural felds in North America. This study describes, for the frst time, the complete mitochondrial genome of H. columbus from South Carolina, USA. Methods: The mitogenome of H. columbus was assembled from Illumina 300 bp pair-end reads. It was annotated and compared to other published mitogenomes of plant-parasitic nematodes in the superfamily Tylenchoidea. The phylogenetic relationships between H. columbus and other 6 genera of plant-parasitic nematodes were examined using protein-coding genes (PCGs). Results: The mitogenome of H. columbus is a circular AT-rich DNA molecule 25,228 bp in length. The annotation result comprises 12 PCGs, 2 ribosomal RNA genes, and 19 transfer RNA genes. No atp8 gene was found in the mitog- enome of H. columbus but long non-coding regions were observed in agreement to that reported for other plant- parasitic nematodes. The mitogenomic phylogeny of plant-parasitic nematodes in the superfamily Tylenchoidea agreed with previous molecular phylogenies. Mitochondrial gene synteny in H. columbus was unique but similar to that reported for other closely related species. Conclusions: The mitogenome of H. columbus is unique within the superfamily Tylenchoidea but exhibits similarities in both gene content and synteny to other closely related nematodes.
    [Show full text]
  • Morphological and Molecular Characterization And
    Turkish Journal of Zoology Turk J Zool (2017) 41: 227-236 http://journals.tubitak.gov.tr/zoology/ © TÜBİTAK Research Article doi:10.3906/zoo-1506-47 Morphological and molecular characterization and phylogenetic position of a new record, Tylenchorhynchus zeae, and some known species of Telotylenchidae Siddiqi, 1960 and Merliniidae Siddiqi, 1971 from Iran 1 1, 1 2 Somaye ALVANI , Esmat MAHDIKHANI-MOGHADAM *, Hamid ROUHANI , Abbas MOHAMMADI 1 Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran 2 Department of Plant Protection, Faculty of Agriculture, University of Birjand, Birjand, Iran Received: 04.07.2015 Accepted/Published Online: 01.07.2016 Final Version: 04.04.2017 Abstract: In order to identify the plant-parasitic nematodes associated with Berberis vulgaris, Crocus sativus, and Ziziphus zizyphus, 360 soil samples were collected from the rhizosphere of these plants in the South Khorasan Province of Iran during 2012–2014. Among the identified species, Tylenchorhynchus zeae Sethi and Swarup, 1968 of Telotylenchidae Siddiqi, 1960 is a new record from Iran and the isolate is described and illustrated based on morphological, morphometric, and molecular characteristics. The phylogenetic tree inferred from partial sequences of the 28S rRNA (D2D3 segments) grouped the Iranian isolate with other T. zeae sequences, forming a strongly supported clade (Bayesian posterior probability: 100%). T. zeae is reported here for the first time on Ziziphus zizyphus for Iranian nematofauna. Additional morphological and molecular information is also provided for other species including Amplimerlinius globigerus, Merlinius brevidens, Pratylenchoides alkani, P. ritteri, Scutylenchus rugosus, S. tartuensis, and Trophurus impar from B. vulgaris, C. sativus, and Z.
    [Show full text]
  • Hirschmannia Ng Differentiated from Radopholusthorne, 1949
    I Nemaiologica 7 (1962) : 197-202. Leiden, E. J. Brill HZRSCHMANNZA N.G. DIFFERENTIATED FROM RADOPHOLUS THORNE, 1949 (NEMATODA : TYLENCHOIDEA ) BY MICHEL LUC AND BASIL GOODEY O.R.S.T.O.M., I.D.E.R.T., Abidjan, Côte d’Ivoire and Rothamsted Experimental Station, Harpenden, England respectively Re-examination of a specimen of Tylenchorhyyncbus spinicaitdattls Sch. Stek., 1944 showed it to be conspecific with Rudopholus 1avubr.i Luc, 1957. This is made the type of Hirschmannia n.g. which also contains H. gracilis n. comb, and H: oryzne n. comb. The lectotype of H. spizicaudata is redescribed and Rndopholur redefined. Schuurmans Stelthoven ( 1944) described two female nematodes, from material collected in the Albert National Park, former Belgian Congo, as Tylenchorhynchus spinicaadatas. The species has been overlooked by nematologists and subsequently not dealt with in either general or specialised papers, though Tarjan (1961) records it. One of us (M.L.) has recently examined one of the original specimens and found that, in two important respects, the original description was inadequate: 1 ) there is a considerable overlap of oesophagus and intestine instead of an abutted junction as figured by Schuurmans Stelchoven (Fig. 1 a, c); 2) the lateral field is 217 of the body-width and is areolated so that each of the four incisures is crenate (Schuurmans Stekhoven reported the lateral fields at 118 of the body- width and not crenate). These clarifications of the form of the oesophagus, combined with the shape of the head, spear and tail, indicate that the species should be transferred from Tylenchorhync,haJJO Radopholtu.
    [Show full text]
  • Australasian Nematology Newsletter
    ISSN 1327-2101 AUSTRALASIAN NEMATOLOGY NEWSLETTER Published by: Australasian Association of Nematologists VOLUME 15 NO. 1 JANUARY 2004 1 From the Editor Thank you to all those who made contributions to this newsletter. July Issue The deadline for the July issue will be June 15th. I will notify you a month in advance so please have your material ready once again. Contacts Dr Mike Hodda President, Australasian Association of Nematologists CSIRO Division of Entomology Tel: (02) 6246 4371 GPO Box 1700 Fax: (02) 6246 4000 CANBERRA ACT 2601 Email: [email protected] Dr Ian Riley Secretary, Australasian Association of Nematologists Department of Applied & Molecular Ecology University of Adelaide Tel: (08) 8303-7259 PMB 1 Fax: (08) 8379-4095 GLEN OSMOND SA 5064 Email: [email protected] Mr John Lewis Treasurer, Australasian Association of Nematologists SARDI, Plant Pathology Unit Tel: (08) 8303 9394 GPO Box 397 Fax: (08) 8303 9393 ADELAIDE SA 5100 Email: [email protected] Ms Jennifer Cobon Editor, Australasian Association of Nematologists Department of Primary Industries Tel: (07) 3896 9892 80 Meiers Road Fax: (07) 3896 9533 INDOOROOPILLY QLD 4068 Email: [email protected] 1 Association News WORKSHOP NOTICE Review of nematode resistance screening/breeding in Australasia A Workshop of the Australasian Association of Nematologists Friday, 6 February 2004 Plant Research Centre, Waite Campus, Urrbrae, South Australia The program will commence with a keynote presentation by Dr Roger Cook – a global overview on development of nematode resistant crops – followed by a series of short presentations by individuals actively involved in screening/breeding for nematode resistance in Australasia.
    [Show full text]
  • JOURNAL of NEMATOLOGY Morphological And
    JOURNAL OF NEMATOLOGY Article | DOI: 10.21307/jofnem-2020-098 e2020-98 | Vol. 52 Morphological and molecular characterization of Heterodera dunensis n. sp. (Nematoda: Heteroderidae) from Gran Canaria, Canary Islands Phougeishangbam Rolish Singh1,2,*, Gerrit Karssen1, 2, Marjolein Couvreur1 and Wim Bert1 Abstract 1Nematology Research Unit, Heterodera dunensis n. sp. from the coastal dunes of Gran Canaria, Department of Biology, Ghent Canary Islands, is described. This new species belongs to the University, K.L. Ledeganckstraat Schachtii group of Heterodera with ambifenestrate fenestration, 35, 9000, Ghent, Belgium. presence of prominent bullae, and a strong underbridge of cysts. It is characterized by vermiform second-stage juveniles having a slightly 2National Plant Protection offset, dome-shaped labial region with three annuli, four lateral lines, Organization, Wageningen a relatively long stylet (27-31 µm), short tail (35-45 µm), and 46 to 51% Nematode Collection, P.O. Box of tail as hyaline portion. Males were not found in the type population. 9102, 6700, HC, Wageningen, Phylogenetic trees inferred from D2-D3 of 28S, partial ITS, and 18S The Netherlands. of ribosomal DNA and COI of mitochondrial DNA sequences indicate *E-mail: PhougeishangbamRolish. a position in the ‘Schachtii clade’. [email protected] This paper was edited by Keywords Zafar Ahmad Handoo. 18S, 28S, Canary Islands, COI, Cyst nematode, ITS, Gran Canaria, Heterodera dunensis, Plant-parasitic nematodes, Schachtii, Received for publication Systematics, Taxonomy. September
    [Show full text]
  • 01F0aea77ca5bfffe05a43057a6a
    Journal of Nematology 49(3):233–235. 2017. Ó The Society of Nematologists 2017. Morphological and Molecular Identification of Longidorus euonymus and Helicotylenchus multicinctus from the Rhizosphere of Grapevine and Banana in Greece 1 2 2 2 EMMANUEL A. TZORTZAKAKIS, CAROLINA CANTALAPIEDRA-NAVARRETE, PABLO CASTILLO, JUAN E. PALOMARES-RIUS, 2 AND ANTONIO ARCHIDONA-YUSTE Abstract: Plant-parasitic nematodes such as Longidorus euonymus and Helicotylenchus multicintctus are species widely distributed in central Europe as well as in Mediterranean area. In Greece, both species have been previously reported but no morphometrics or molecular data were available for these species. Nematode surveys in the rhizosphere of grapevines in Athens carried out in 2016 and 2017, yielded a Longidorus species identified as Longidorus euonymus. Similarly, a population of Helicotylenchus multicinctus was detected infecting banana roots from an outdoor crop in Tertsa, Crete. For both species, morphometrics and molecular data of Greek populations were provided, resulting in the first integrative identification of both nematode species based on morphometric and molecular markers, confirming the occurrence of these two nematodes in Greece as had been stated in earlier reports. Key words: detection, needle and spiral nematodes, rDNA. Longidorus euonymus Mali and Hooper, 1974 is a spe- was found at a density of five nematodes per gram of cies widely distributed in central and south Europe root, after incubating macerated root pieces in modi- found in a wide range of hosts including perennial, fied Baerman funnels. woody, annual and herbaceous plants (Oro et al., Specimens to be observed under light microscopy 2005). In Greece, the only report of this nematode is were heat killed by adding hot 4% formaldehyde solu- from artichoke crop at Marathon area near Athens, but tion and processed to pure glycerin using De Grisse’s no molecular data are available for this population (1969) method.
    [Show full text]
  • Nematode Management in Lawns
    Agriculture and Natural Resources FSA6141 Nematode Management in Lawns Aaron Patton Nematodes are pests of lawns in Assistant Professor - Arkansas, particularly in sandy soils. Turfgrass Specialist Nematodes are microscopic, unseg­ mented roundworms, 1/300 to 1/3 inch David Moseley in length (8) (Figure 1), that live in County Extension Agent ­ the soil and can parasitize turfgrasses. Agriculture Ronnie Bateman Pest Management Anus Program Associate Figure 2. Parasitic nematode feeding on Head a plant root (Photo by U. Zunke, Nemapix Tail Vol. 2) Terry Kirkpatrick Stylet Professor - Nematologist There are six stages in the nematode life cycle including an egg stage and the adult. There are four Figure 1. Schematic drawing of a plant juvenile stages that allow the parasitic nematode (Adapted from N.A. nematode to increase in size and in Cobb, Nemapix Vol. 2) some species to change shape. These juvenile stages are similar to the Although most nematodes are larval stages found in insects. beneficial and feed on fungi, bacteria Nematodes are aquatic animals and, and insects or help in breaking down therefore, require water to survive. organic matter, there are a few Nematodes live and move in the water species that parasitize turfgrasses and film that surrounds soil particles. Soil cause damage, especially in sandy type, particularly sand content, has a soils. All parasitic nematodes have a major impact on the ability of nema­ stylet (Figure 1), a protruding, needle­ todes to move, infect roots and repro­ like mouthpart, which is used to punc­ duce. For most nematodes that are a ture the turfgrass root and feed problem in turf, well-drained sandy (Figure 2).
    [Show full text]
  • Transcriptome Profiling of the Root-Knot Nematode Meloidogyne Enterolobii During Parasitism and Identification of Novel Effector Proteins
    Ecole Doctorale de Sciences de la Vie et de la Santé Unité de recherche : UMR ISA INRA 1355-UNS-CNRS 7254 Thèse de doctorat Présentée en vue de l’obtention du grade de docteur en Biologie Moléculaire et Cellulaire de L’UNIVERSITE COTE D’AZUR par NGUYEN Chinh Nghia Etude de la régulation du transcriptome de nématodes parasites de plante, les nématodes à galles du genre Meloidogyne Dirigée par Dr. Bruno FAVERY Soutenance le 8 Décembre, 2016 Devant le jury composé de : Pr. Pierre FRENDO Professeur, INRA UNS CNRS Sophia-Antipolis Président Dr. Marc-Henri LEBRUN Directeur de Recherche, INRA AgroParis Tech Grignon Rapporteur Dr. Nemo PEETERS Directeur de Recherche, CNRS-INRA Castanet Tolosan Rapporteur Dr. Stéphane JOUANNIC Chargé de Recherche, IRD Montpellier Examinateur Dr. Bruno FAVERY Directeur de Recherche, UNS CNRS Sophia-Antipolis Directeur de thèse Doctoral School of Life and Health Sciences Research Unity: UMR ISA INRA 1355-UNS-CNRS 7254 PhD thesis Presented and defensed to obtain Doctor degree in Molecular and Cellular Biology from COTE D’AZUR UNIVERITY by NGUYEN Chinh Nghia Comprehensive Transcriptome Profiling of Root-knot Nematodes during Plant Infection and Characterisation of Species Specific Trait PhD directed by Dr Bruno FAVERY Defense on December 8th 2016 Jury composition : Pr. Pierre FRENDO Professeur, INRA UNS CNRS Sophia-Antipolis President Dr. Marc-Henri LEBRUN Directeur de Recherche, INRA AgroParis Tech Grignon Reporter Dr. Nemo PEETERS Directeur de Recherche, CNRS-INRA Castanet Tolosan Reporter Dr. Stéphane JOUANNIC Chargé de Recherche, IRD Montpellier Examinator Dr. Bruno FAVERY Directeur de Recherche, UNS CNRS Sophia-Antipolis PhD Director Résumé Les nématodes à galles du genre Meloidogyne spp.
    [Show full text]