DOCSLIB.ORG

Molecular Systematics of Mahseers (Cyprinidae) in Malaysia Inferred from Sequencing of a Mitochondrial Cytochrome C Oxidase I (COI) Gene

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Molecular Systematics of Mahseers (Cyprinidae) in Malaysia Inferred from Sequencing of a Mitochondrial Cytochrome C Oxidase I (COI) Gene Pertanika J. Trop. Agric. Sci. 31(2): 263 - 269 (2008) ISSN: 1511-3701 ©Universiti Putra Malaysia Press Molecular Systematics of Mahseers (Cyprinidae) in Malaysia Inferred from Sequencing of a Mitochondrial Cytochrome C Oxidase I (COI) Gene Yuzine B. Esa1,2*, Siti Shapor Siraj2, Siti Khalijah Daud2, Japning Jeffrine Rovie Ryan3, Khairul Adha A. Rahim1 and Soon Guan, Tan2 1Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia 2Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia 3Institute of Biodiversity, Bukit Rengit, 28500 Lanchang, Pahang, Malaysia *E-mail: [email protected] ABSTRACT This study examined the molecular systematics among three Mahseers (Tor douronensis, Tor tambroides and Neolissochilus stracheyi) using partial sequencing of a Cytochrome C Oxidase I (COI) mitochondrial DNA segment (466bp). The phylogenetic results using the Neighbour-Joining (NJ) method supported the monophyletic status (hence the taxonomic status) among the three putative Mahseer species. The close genetic relationships (0.1- 0.4%) found between T. tambroides samples from Peninsular Malaysia (kelah fish) and those from Sarawak (empurau fish) also supported their classification as belonging to the same species. The phylogenetic analysis also showed that the T. douronensis mtDNA consisted of three highly distinct lineages supported by high bootstrap values, with the Sabah samples forming its own cluster. Thus, this phylogenetic study, although based on a limited number of samples and only a single mtDNA gene managed to provide useful insights into the systematic status of the Mahseers found in Malaysia. Keywords: Freshwater fish, Mahseers, COI, molecular systematics INTRODUCTION decreasing rapidly due to environmental Freshwater fishes of the genus Tor Gray, degradation and increased fishing pressure (Ng, commonly known as the Mahseers, belong to 2004). the family Cyprinidae (subfamily Cyprininae) So far, very little taxonomic work to (Inger and Chin, 1962; Mohsin and Ambak, systematically sort out Malaysian Mahseer has 1983; Roberts, 1989; Kottelat et al., 1993). There been documented. The most cited work was by are currently 17 described species under the Mohsin and Ambak (1983) who described Tor genus Tor from all across Asia (Ng, 2004) but tambroides and Tor soro as two valid Mahseers only three species were reported in Malaysia: Tor found in Peninsular Malaysia while a more recent tambroides Bleeker, Tor tambra Valenciennes, and view by Ng (2004) suggested the occurrence of Tor douronensis Valenciennes (Kottelat et al., 1993; three species; T. tambroides, T. tambra and T. Kottelat and Whitten, 1996; Ng, 2004). The douronensis. Other taxonomic works recognized taxonomic status of Tor soro Valenciennes had T. douronensis and T. tambroides as two valid species been revised and it is currently re-classified as (Roberts, 1989; Kottelat et al., 1993; Rainboth, Neolissochilus stracheyi (Rainboth, 1996). Mahseers 1996; Zhou and Chu, 1996), although Roberts are important as food fish as well as ornamental (1999) classified them to be a single species, and and recreational fishes. However, recently the a junior synonym to T. tambra. The presence of population sizes of their natural stocks are the median lobe has been characterized as a Received: 12 July 2007 Accepted: 30 June 2008 * Corresponding Author 14. 25/2008-Yuzine 263 24/3/05, 9:56 AM Yuzine B. Esa et al. diagnostic morphological character distinguishing the present study also aimed to clarify the the genus Tor from the genus Neolissochillus phylogenetic relationships among Mahseer fishes (Rainboth, 1996; Ng, 2004), though it cannot be in Malaysia but with a few different approaches. used consistently as a marker to discriminate First, we utilized direct sequencing of the between fishes of the genus Tor. Thus, the Cytochrome C Oxidase I (COI) mtDNA gene region, application of molecular techniques (such as DNA a gene with a faster evolutionary rate compared sequencing) can provide new and better insights to the 16S rRNA (Simon et al., 1994), and thus into the unresolved taxonomy and phylogenetic capable of providing a better resolution at the relationships of all the putative Mahseers in interspecific level. Secondly, we included Malaysia (Nguyen et al., 2006). additional Tor samples from Peninsular Malaysia Nguyen et al. (2006) recently produced the (kelah fish) and Sabah (pelian fish), to compare first molecular work on Mahseers in Malaysia by with the T. douronensis (semah fish) and T. examining the genetic diversity and phylogenetic tambroides (empurau fish) of Sarawak. Thirdly, relationships of broodstocks of T. douronensis N. stracheyi representing the genus Neolissochilus and T. tambroides cultured in Sarawak (Borneo) were included in the phylogenetic study to through sequencing analysis of the mitochondrial quantify the genetic differences between the two DNA (mtDNA) 16S rRNA gene region. Thus, genera. Fig. 1: Map showing sampling locations and sample size (N) in Peninsular Malaysia, Sarawak and Sabah. TD= T. douronensis, TT= T. tambroides, NS= N. stracheyi 264 Pertanika J. Trop. Agric. Sci. Vol. 31(2) 2008 14. 25/2008-Yuzine 264 24/3/05, 9:56 AM Molecular Systematics of Mahseers in Malaysia Inferred from Sequencing of a Mitochondrial Cytochrome COI Gene MATERIALS AND METHODS between populations was calculated using the A total of 111 individuals of the three putative Tamura-Nei distance (Tamura and Nei, 1993), mahseers (T. douronensis, T. tambroides and N. based on unequal base frequencies and unequal stracheyi) were collected from several locations in ratios of transition to transversion (Ti:Tv) Peninsular Malaysia, Sarawak and Sabah (Fig. 1). implemented in MEGA. Total DNA was extracted using a modified CTAB method (Grewe et al., 1993) in the presence of RESULTS AND DISCUSSION Proteinase K. The isolated genomic DNA was The sequence analysis of the partial COI gene used for the mtDNA analysis. (466 base pairs) revealed a total of 24 haplotypes A 500 bp segment of the COI gene was in the nucleotide data set: 14 haplotypes amplified with the oligonucleotide primers COIf belonging to T. douronensis, six haplotypes (5’ CCTGCAGGAGGAGGAGAYCC 3’, forward) belonging to T. tambroides and four haplotypes and COIe (5’ CCAGAGATTAGA GGGAATC AG belonging to N. stracheyi (Fig. 2). The sequence TG 3’, reverse) (Palumbi et al. 1991). of each of the haplotypes was deposited in the Approximately, 50-100 ng of the template DNA GenBank (GeneBank Reference Numbers: was amplified in a 25 ml reaction mixture DQ532824-DQ532827 and EF192444-192463). In containing 50 mM 10X buffer, 2 mM MgCl2, 0.2 total, 74 (15.9%) variable sites were found, of mM of each dNTP (Promega), 0.1 mM of each which 56 (12.0%) were parsimony informative primer, and 0.5 units of Taq DNA Polymerase sites, while 392 (84.1%) were monomorphic sites. (Promega). The cycle parameters consisted of Transitional changes occurred more frequently 35 cycles of denaturation (95oC, 30 seconds), than transversional changes as is typical of animal annealing (45oC, 30 seconds), and extension mitochondrial genomes (Briolay et al., 1998). (72oC, 60 seconds). The amplified products were The phylogenetic results obtained by using visualized on 1% agarose gel containing ethidium the NJ method supported the monophyletic bromide, run for approximately 30 min at 90 V status among the three mahseers (Fig. 3), and photographed under UV light. The purified although the bootstrap support between T. PCR products were directly sequenced using the tambroides and N. stracheyi was low (58%). The BigDye® Terminator v3.0 Cycle Sequencing kit high genetic divergence separating T. douronensis (ACGT) on an ABI 377 automated sequencer and T. tambroides confirmed their status as distinct (PE Applied Biosystem) using only the forward species (Table 1). Likewise, the high genetic primer (COIf). Sequencing reaction using the divergence separating the N. stracheyi lineage reverse primer (COIe) was subsequently carried from the Tor lineages (7.7-8.7%) also supported out on some of the samples (haplotypes) to its recent reclassification from the genus Tor verify the polymorphism in the DNA sequence into the genus Neolissochilus (Rainboth, 1996). initially detected using the forward primer. The close genetic relationships (0.1-0.4%) The CHROMAS (Version 1.45) program was found between T. tambroides samples from used to display the fluorescence-based DNA Peninsular Malaysia (kelah fish) and those from sequencing analysis results. The multiple Sarawak (empurau fish) supported their sequence alignments were done using the taxonomic status as belonging to the same species CLUSTAL X program version 1.81 (Thompson (Table 1). However, the overall very low level of et al., 1997), and subsequently aligned by eye. genetic differentiation within and among T. The Molecular Evolutionary Genetics Analysis tambroides populations may have resulted from (MEGA) version 3.1 (Kumar et al., 2004) program the limited number of samples (2-17) analysed was used to construct a neighbour-joining (NJ) for each population, but was consistent with (Saitou and Nei, 1987) tree using two indigenous those found by Nguyen et al. (2006) in the cyprinids (Barbonymus gonionotus (Genbank Sarawak populations. accession number: DQ532806) and Barbonymus The phylogenetic analysis also revealed that schwanenfeldii (Genbank accession number: the T. douronensis mtDNA consisted of three DQ532805)) as outgroup
Load more

Recommended publications
  • Π·¡— Πà È”°Õ„Àà ‡°Â ¥§«“¡ ¡∫‘ √≥Ÿ ¢Õß™Πå ¥¢Õߪ≈“‘ Adaptations in River Fishes Facilitate Species Richness
    °“√ª√∫μ— «¢Õߪ≈“„π·¡— πà È”°Õ„Àà ‡°â ¥§«“¡ ¡∫‘ √≥Ÿ ¢Õß™πå ¥¢Õߪ≈“‘ Adaptations in River Fishes Facilitate Species Richness ‡ø¥‡¥Õ√§‘ «≈‡≈‘ ¬¡’ ‡Œπ√ ’ ∫¡’ ™‘ 1* æ™√“— π∏‘ ‚√®π‘ ¿å °¥— 2’ √ß∑ÿà 欑 å ‚æ≈߇»√…∞â 2’ ·≈– ‡æ¬ß„®’ ™ππ∑√¿‘ ¡Ÿ 2‘ 1«∑¬“»“ μ√‘ å ß·«¥≈‘Ë Õ¡â §≥–«∑¬“»“ μ√‘ å ¡À“«∑¬“≈‘ ¬∫— √æ“Ÿ 2‚§√ß°“√∫≥±— μ»‘ °…“÷ “¢“«∑¬“»“ μ√‘ å ß·«¥≈‘Ë Õ¡â §≥–«∑¬“»“ μ√‘ å ¡À“«∑¬“≈‘ ¬∫— √æ“Ÿ F.William H. Beamish1*, Patchara Nithirojpakdee2, Rungthip Plongsesthee2 and Peangchai Chanintarapoomi2 1Environmental Science Program, Faculty of Science, Burapha University, 2Graduate Program, Environmental Science , Faculty of Science, Burapha University. ∫∑§¥¬— Õà ·π«§«“¡§‘¥¢Õß ‘Ëß·«¥≈âÕ¡ (The cooncept of environmental) °“√ª√—∫μ—«∑“ߥâ“π°“√°‘πÕ“À“√ ‡ªìπ·π«§‘¥∑’Ë¡’ §«“¡ ¡æ— π∏— ‡°å ¬«‡π’Ë Õß°◊Ë π°— ∫≈— °…≥–∑“ßø— ï‚π‰∑ª á À√Õ◊ ®’‚π‰∑ª á ´ß∂÷Ë Õ‡ª◊ π ì ß ”§‘Ë ≠∑— ™’Ë «¬„Àà â ß¡‘Ë ™’ «’ μμ‘ “ßÊà “¡“√∂¥”√ß™«’ μ·≈–‘ √—∫¡◊Õ°—∫≈—°…≥–Õ—π®”‡æ“–¢Õß·μà≈–·À≈àß∑’ËÕ¬ŸàÕ“»—¬‰¥â¥’¢÷Èπ √«¡∑—Èß ‘Ëß¡’™’«‘μÕ◊ËπÊ ¥â«¬ ´÷Ëß®–¡’ª√–‚¬™πå„π«ß°«â“ß ”À√—∫°“√ ∑”§«“¡‡¢â“„®‡°’ˬ«°—∫§«“¡™ÿ°™ÿ¡¢Õß ‘Ëß¡’™’«‘μ°—∫°“√Õ¬Ÿà√à«¡°—π¢Õß ‘Ëß¡’™’«‘μ „π ¿“«–·«¥≈âÕ¡∑’Ë¡’°“√‡ª≈’ˬπ·ª≈߉ª¡“Õ¬Ÿà μ≈Õ¥‡«≈“¢Õß·À≈ßπà È”„πª√–‡∑»‰∑¬ Õπ‡ª— π·À≈ì ß∑à Õ¬’Ë Õ“»Ÿà ¬¢Õߪ≈“— ∑”„Àª≈“μâ Õߪ√â ∫μ— «‡æ— Õ„À◊Ë ‡¢â “°â ∫§— ≥≈ÿ °…≥–∑“߇§¡— °“¬¿“æ’ Õ“®°Õ„Àà ‡°â ¥§«“¡À≈“°À≈“¬¢Õß ‘ ß¡‘Ë ™’ «’ μ¡“°¢‘ π÷È °“√» °…“„π§√÷ ßπ—È ®’È ß¡÷ «’ μ∂— ª√– ߧÿ ‡æå Õ»◊Ë °…“≈÷ °…≥–∑“߇§¡— °“¬¿“æ„π·À≈’ ßπà È”μ“ßÊà „π¿“§°≈“ߢÕߪ√–‡∑»‰∑¬ ∑¡’Ë º≈μ’ Õª≈“ Õß«ß»à å ´ßÕ“»÷Ë ¬√— «¡°à π„π·À≈— ßπà È”‡¥¬«°’ π— §Õ◊ «ß»ª≈“μ–‡æå ¬π’ (Cyprinidae) ·≈– «ß»å ª≈“®ß®°‘È
    [Show full text]
  • Download Download
    :ŽƵƌŶĂůŽĨdŚƌĞĂƚĞŶĞĚdĂdžĂͮǁǁǁ͘ƚŚƌĞĂƚĞŶĞĚƚĂdžĂ͘ŽƌŐͮϮϲDĂLJϮϬϭϰͮϲ;ϱͿ͗ϱϲϴϲʹϱϲϵϵ ®ÝãÙ®çã®ÊÄ͕ã«ÙãÝÄÊÄÝÙòã®ÊÄÝããçÝʥ㫠ÊÃÃçÄ®ã®ÊÄ tùÄD«ÝÙ͕Eʽ®ÝÝÊ«®½çÝóùÄÄÝ®Ý;ù͕ϭϴϳϯͿ /^^E ;d½ÊÝã®͗ùÖٮĮͿ͗ÄÄî½Ù¦Ùʥ㫠KŶůŝŶĞϬϵϳϰʹϳϵϬϳ WƌŝŶƚϬϵϳϰʹϳϴϵϯ tÝãÙÄ'«ãÝ͕/Ä® KWE^^ ŶǀĂƌůŝϭ͕EĞĞůĞƐŚĂŚĂŶƵŬĂƌϮ͕^ŝďLJWŚŝůŝƉϯ͕<͘<ƌŝƐŚŶĂŬƵŵĂƌϰΘZĂũĞĞǀZĂŐŚĂǀĂŶϱ ϭ͕ϯ͕ϰ͕ϱŽŶƐĞƌǀĂƟŽŶZĞƐĞĂƌĐŚ'ƌŽƵƉ;Z'Ϳ͕^ƚ͘ůďĞƌƚ͛ƐŽůůĞŐĞ͕<ŽĐŚŝ͕<ĞƌĂůĂϲϴϮϬϭϴ͕/ŶĚŝĂ Ϯ/ŶĚŝĂŶ/ŶƐƟƚƵƚĞŽĨ^ĐŝĞŶĐĞĚƵĐĂƟŽŶĂŶĚZĞƐĞĂƌĐŚ;//^ZͿ͕ƌ͘,ŽŵŝŚĂďŚĂZŽĂĚ͕WĂƐŚĂŶ͕WƵŶĞ͕ DĂŚĂƌĂƐŚƚƌĂϰϭϭϬϬϴ͕/ŶĚŝĂ Ϯ͕ϱ^LJƐƚĞŵĂƟĐƐ͕ĐŽůŽŐLJΘŽŶƐĞƌǀĂƟŽŶ>ĂďŽƌĂƚŽƌLJ͕ŽŽKƵƚƌĞĂĐŚKƌŐĂŶŝnjĂƟŽŶ;KKͿ͕ϵϲ<ƵŵƵĚŚĂŵEĂŐĂƌ͕ sŝůĂŶŬƵƌŝĐŚŝZŽĂĚ͕ŽŝŵďĂƚŽƌĞ͕dĂŵŝůEĂĚƵϲϰϭϬϯϱ͕/ŶĚŝĂ ϯĞƉĂƌƚŵĞŶƚŽĨŽŽůŽŐLJ͕EŝƌŵĂůĂŐŝƌŝŽůůĞŐĞ͕<ŽŽƚŚƵƉĂƌĂŵďƵ͕<ĂŶŶƵƌ͕<ĞƌĂůĂϲϳϬϳϬϭ͕/ŶĚŝĂ ϱDĂŚƐĞĞƌdƌƵƐƚ͕ĐͬŽdŚĞ&ƌĞƐŚǁĂƚĞƌŝŽůŽŐŝĐĂůƐƐŽĐŝĂƟŽŶ͕ĂƐƚ^ƚŽŬĞZŝǀĞƌ>ĂďŽƌĂƚŽƌLJ͕tĂƌĞŚĂŵ͕ ŽƌƐĞƚ͕,ϮϬϲ͕hŶŝƚĞĚ<ŝŶŐĚŽŵ ϭĂŶǀĂƌĂůŝŝĨΛŐŵĂŝů͘ĐŽŵ͕ϮŶ͘ĚĂŚĂŶƵŬĂƌΛŝŝƐĞƌƉƵŶĞ͘ĂĐ͘ŝŶ͕ϯƉŚŝůŝƉƐŝďLJΛŐŵĂŝů͘ĐŽŵ͕ϰŬŬĂƋƵĂΛŐŵĂŝů͘ĐŽŵ͕ ϱƌĂũĞĞǀƌĂƋΛŚŽƚŵĂŝů͘ĐŽŵ;ĐŽƌƌĞƐƉŽŶĚŝŶŐĂƵƚŚŽƌͿ ďƐƚƌĂĐƚ͗dŚĞtĂLJĂŶĂĚDĂŚƐĞĞƌNeolissochilus wynaadensis ;ĂLJ͕ϭϴϳϯͿŝƐĂŶĞŶĚĞŵŝĐĐLJƉƌŝŶŝĚĮƐŚƚŚĂƚŽĐĐƵƌƐŝŶƚŚĞƵƉůĂŶĚƐƚƌĞĂŵƐ ĂŶĚƌŝǀĞƌƐŽĨƚŚĞƐŽƵƚŚĞƌŶƌĞŐŝŽŶŽĨƚŚĞtĞƐƚĞƌŶ'ŚĂƚƐ͘dŚŝƐƐƉĞĐŝĞƐŚĂƐďĞĞŶůŝƐƚĞĚĂƐ͚ƌŝƟĐĂůůLJŶĚĂŶŐĞƌĞĚ͛ŽŶƚŚĞ/hEZĞĚ>ŝƐƚŽĨ dŚƌĞĂƚĞŶĞĚ^ƉĞĐŝĞƐĚƵĞƚŽŝƚƐƌĞƐƚƌŝĐƚĞĚĚŝƐƚƌŝďƵƟŽŶĂŶĚŚĞĂǀLJĚĞĐůŝŶĞƐŝŶƉŽƉƵůĂƟŽŶƐ͘>ŝŬĞŵĂŶLJůĂƌŐĞĐLJƉƌŝŶŝĚƐŽĨƚŚĞtĞƐƚĞƌŶ'ŚĂƚƐ͕ N. wynaadensis ŝƐƉŽŽƌůLJŬŶŽǁŶĂŶĚĚŽĐƵŵĞŶƚĞĚ͕ǁŝƚŚǀĞƌLJĨĞǁǀĞƌŝĮĞĚƌĞĐŽƌĚƐĂŶĚǀŽƵĐŚĞƌƐƉĞĐŝŵĞŶƐ͘ĂƐĞĚŽŶƐƉĞĐŝŵĞŶƐƌĞĐĞŶƚůLJ ĐŽůůĞĐƚĞĚ ĨƌŽŵ tĂLJĂŶĂĚ͕ <ĞƌĂůĂ͕ ƚŚĞ ƚLJƉĞ ůŽĐĂůŝƚLJ͕ĂƐ ǁĞůů ĂƐ ƚǁŽ ĂĚĚŝƟŽŶĂů ůŽĐĂƟŽŶƐ ŝŶ ƚŚĞ <ŽĚĂŐƵ ŝƐƚƌŝĐƚ ŽĨ <ĂƌŶĂƚĂŬĂ͖ ǁĞ ƉƌŽǀŝĚĞ ŝŶĨŽƌŵĂƟŽŶŽŶƚŚĞĐƵƌƌĞŶƚĚŝƐƚƌŝďƵƟŽŶ͕ƉŚLJůŽŐĞŶĞƟĐƉŽƐŝƟŽŶ͕ƚŚƌĞĂƚƐĂŶĚĐŽŶƐĞƌǀĂƟŽŶ͘ŶƵƉĚĂƚĞĚĐŽŶƐĞƌǀĂƟŽŶĂƐƐĞƐƐŵĞŶƚŽĨƚŚŝƐ
    [Show full text]
  • National Report on the Fish Stocks and Habitats of Regional, Global
    United Nations UNEP/GEF South China Sea Global Environment Environment Programme Project Facility NATIONAL REPORT on The Fish Stocks and Habitats of Regional, Global, and Transboundary Significance in the South China Sea THAILAND Mr. Pirochana Saikliang Focal Point for Fisheries Chumphon Marine Fisheries Research and Development Center 408 Moo 8, Paknum Sub-District, Muang District, Chumphon 86120, Thailand NATIONAL REPORT ON FISHERIES – THAILAND Table of Contents 1. MARINE FISHERIES DEVELOPMENT........................................................................................2 / 1.1 OVERVIEW OF THE FISHERIES SECTOR ...................................................................................2 1.1.1 Total catch by fishing area, port of landing or province (by species/species group).7 1.1.2 Fishing effort by gear (no. of fishing days, or no. of boats) .......................................7 1.1.2.1 Trawl ...........................................................................................................10 1.1.2.2 Purse seine/ring net....................................................................................10 1.1.2.3 Gill net.........................................................................................................12 1.1.2.4 Other gears.................................................................................................12 1.1.3 Economic value of catch..........................................................................................14 1.1.4 Importance of the fisheries sector
    [Show full text]
  • Biodiversity Assessment of the Mekong River in Northern Lao PDR: a Follow up Study
    ���� ������������������ ������������������ Biodiversity Assessment of the Mekong River in Northern Lao PDR: A Follow Up Study October, 2004 WANI/REPORT - MWBP.L.W.2.10.05 Follow-Up Survey for Biodiversity Assessment of the Mekong River in Northern Lao PDR Edited by Pierre Dubeau October 2004 The World Conservation Union (IUCN), Water and Nature Initiative and Mekong Wetlands Biodiversity Conservation Programme Report Citation: Author: ed. Dubeau, P. (October 2004) Follow-up Survey for Biodiversity Assessment of the Mekong River in Northern Lao PDR, IUCN Water and Nature Initiative and Mekong Wetlands Biodiversity Conservation and Sustainable Use Programme, Bangkok. i The designation of geographical entities in the book, and the presentation of the material, do not imply the expression of any opinion whatsoever on the part of the Mekong Wetlands Biodiversity Conservation and Sustainable Use Programme (or other participating organisations, e.g. the Governments of Cambodia, Lao PDR, Thailand and Viet Nam, United Nations Development Programme (UNDP), The World Conservation Union (IUCN) and Mekong River Commission) concerning the legal status of any country, territory, or area, or of its authorities, or concerning the delimitation of its frontiers or boundaries. The views expressed in this publication do not necessarily reflect those of the Mekong Wetlands Biodiversity Programme (or other participating organisations, e.g. the Governments of Cambodia, Lao PDR, Thailand and Viet Nam, UNDP, The World Conservation Union (IUCN) and Mekong River
    [Show full text]
  • Schistura Aurantiaca , a New Species from the Mae Khlong Basin
    169 Ichthyol. Explor. Freshwaters, Vol. 22, No. 2, pp. 169-178, 7 figs., 2 tabs., June 2011 © 2011 by Verlag Dr. Friedrich Pfeil, München, Germany – ISSN 0936-9902 Schistura aurantiaca, a new species from the Mae Khlong basin, Thailand (Teleostei: Nemacheilidae) Rungthip Plongsesthee*, Lawrence M. Page** and William Beamish* Schistura aurantiaca, new species, is described from the Mae Khlong basin in western Thailand. It is distinguished from all other species of Schistura by a unique color pattern of 3-9 orange bars on the side of the body, with the 1st bar immediately behind the head and the 2nd bar near the dorsal-fin origin and widely separated so that most of the nape and the anterior side of the body are uniformly brown. The species reaches only 41 mm SL and in- habits shallow gravel and rubble riffles in small streams. Introduction information on their distribution and ecology. Subsequently, three species have been described Schistura is an exceptionally diverse genus con- from Thailand (Kottelat, 1990b; Vidthayanon, taining about 180 species. Oddly, the genus is 2003; Bohlen & Šlechtová, 2009), 50 species from present in Borneo (Roberts, 1989; reported as Laos (Kottelat, 1998, 2000; Vidthayanon & Jarutha- Nemacheilus maculiceps), but absent in Sumatra, nin, 2002), and 16 species from Vietnam (Freyhof Java and the southern Malay Peninsula (Bohlen & Serov, 2001; Kottelat, 2004; Nguyen, 2005; & Šlechtová, 2009). As phylogenetic information Nguyen & Nguyen, 2007). Recent collections from and more complete distributional data accumu- the Mae Khlong basin in western Thailand include late, the exceptional diversity and endemism of another new species of Schistura that is described Schistura will provide excellent material for the herein.
    [Show full text]
  • Deep Metazoan Phylogeny 2011 New Data, New Challenges
    1 Zitteliana An International Journal of Palaeontology and Geobiology Series B /Reihe B Abhandlung der Bayerischen Staatssammlung für Paläontologie und Geologie 30 Deep Metazoan Phylogeny 2011 New data, new challenges D M P München 11. – 14. October2011 2011 DeepProgramme Metazoan and Phylogeny Abstracts 2011 New data, new challenges Munich 11–14 October 2011 SPP 1174Munich "Deep Metazoan 2011 Phylogeny" www.palmuc.de/dmp2011 Paläontologie Bayerische GeoBio- & Geobiologie Staatssammlung Center LMU München für Paläontologie und Geologie LMU München 2 Editors-in-Chief: Gert Wörheide, Michael Krings Production and Layout: Martine Focke Bayerische Staatssammlung für Paläontologie und Geologie Editorial Board A. Altenbach, Munich, Germany B.J. Axsmith, Mobile, AL, USA F.T. Fürsich, Erlangen, Germany K. Heißig, Munich, Germany H. Kerp, Münster, Germany J. Kriwet, Vienna, Austria J.H. Lipps, Berkeley, CA, USA T. Litt, Bonn, Germany A. Nützel, Munich, Germany O.W.M. Rauhut, Munich, Germany B. Reichenbacher, Munich, Germany J.W. Schopf, Los Angeles, CA, USA G. Schweigert, Stuttgart, Germany F. Steininger, Eggenburg, Austria Bayerische Staatssammlung für Paläontologie und Geologie Richard-Wagner-Str. 10, D-80333 München, Deutschland http://www.palmuc.de email: [email protected] Authors are solely responsible for the contents of their articles. Copyright © 2011 Bayerische Staassammlung für Paläontologie und Geologie, München Articles published in Zitteliana are protected by copyright. Reprint and duplications via photochemical, electronical and other ways and production of translations or usage of the presentations for radio television broadcasting or internet remain – even in extracts – subject to the Bayerische Staatssammlung für Paläontologie und Geologie, Munich. A permission in written form is required in advance.
    [Show full text]
  • Fishes of the Xe Kong Drainage in Laos, Especially from the Xe Kaman
    1 Co-Management of freshwater biodiversity in the Sekong Basin Fishes of the Xe Kong drainage in Laos, especially from the Xe Kaman October 2011 Maurice Kottelat Route de la Baroche 12 2952 Cornol Switzerland [email protected] 2 Summary The fishes of the Xe Kaman drainage in Laos have been surveyed between 15 and 24 May 2011. Fourty-five fish species were observed, bringing to 175 the number of species recorded from the Xe Kong drainage in Laos, 9 of them new records for the drainage. Twenty-five species (14 %) have been observed from no other drainage and are potentially endemic to the Xe Kong drainage. Five species observed during the survey are new to science (unnamed); they belong to the genera Scaphiodonichthys, Annamia, Sewellia and Schistura (2 species). Three of them have been discovered during the survey, the others although still unnamed were already known for some time, under an erroneous name. In the Xekong drainage, a total of 19 (11 %) fish species are still unnamed or their identity is not yet cleared and they are potentially also new to science. The survey focused on Dakchung district. Eleven species were collected on Dakchung plateau and 3 are apparently new to science (and thus 27 % of the fish fauna of the plateau is endemic there). Most of the endemic species (and all the new species discovered by the survey) are from rapids and other high gradient habitats. This reflects the limited distribution range of rheophilic species, but may also partly result from a sampling bias. Acknowledgements The author wishes to thank the WWF – Co-management of Freshwater Biodiversity in the Sekong Basin Project funded by the Critical Ecosystem Partnership FUND (CEPF) for supporting and organising this survey, especially Dr Victor Cowling who originally developed the survey activity and Mr.
    [Show full text]
  • Cyprinid Fishes of the Genus Neolissochilus in Peninsular Malaysia
    Zootaxa 3962 (1): 139–157 ISSN 1175-5326 (print edition) www.mapress.com/zootaxa/ Article ZOOTAXA Copyright © 2015 Magnolia Press ISSN 1175-5334 (online edition) http://dx.doi.org/10.11646/zootaxa.3962.1.7 http://zoobank.org/urn:lsid:zoobank.org:pub:774A52BB-DD78-467E-ADA0-785B75E48558 Cyprinid fishes of the genus Neolissochilus in Peninsular Malaysia M. Z. KHAIRONIZAM1, M. ZAKARIA-ISMAIL & JONATHAN W. ARMBRUSTER2 1School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia. E-mail: [email protected] 2Department of Biological Sciences, 101 Life Sciences Building, Auburn University, Auburn, AL 36849, USA. E-mail: [email protected]. Email of Corresponding Author: [email protected] Abstract Meristic, morphometric and distributional patterns of cyprinid fishes of the genus Neolissochilus found in Peninsular Ma- laysia are presented. Based on the current concept of Neolissochilus, only two species are present: N. soroides and N. hen- dersoni. Neolissochilus hendersoni differs from N. soroides by having lower scale and gill raker counts. Neolissochilus soroides has three mouth types (normal with a rounded snout, snout with a truncate edge, and lobe with a comparatively thick lower lip). A PCA of log-transformed measurements did not reveal significant differences between N. hendersoni and N. soroides, or between any of the morphotypes of N. soroides; however, a CVA of log-transformed measurements successfully classified 87.1% of all specimens. Removing body size by running a CVA on all of the principal components except PC1 (which was correlated with length) only slightly decreased the successful classification rate to 86.1%. Differ- ences in morphometrics were as great between the three morphotypes of N.
    [Show full text]
  • Environmentasia
    EnvironmentAsia AvailableAvailable online online at www.tshe.org/EA at www.tshe.org/EA The international journal published by the Thai Society of Higher Education Institutes on Environment EnvironmentAsiaEnvironmentAsia 2 5(1)(2009) (2012) 50-54 1-16 Genotoxicity Assessment of Mercuric Chloride in the Marine Fish Therapon jaruba Why Thai River Fish Occur Where They are Found Nagarajan Nagarani, Arumugam Kuppusamy Kumaraguru, Velmurugan Janaki Devi F. William H. Beamish a,b, Punnatutand Chandrasekaran Archana Devi Kangrang b, Patchara Nithirojpakdee b and Rungthip Plongsesthee b a Center Department for Marine of and Integrative Coastal Biology, Studies, College School ofof Biological Energy, Environment Science, University and Natural of Guelph, Resources, Madurai KamarajGuelph, Ontario, University, Canada, Madurai-625021, N1G2W1 India b Environmental Science Program and Centre of Excellence on Environmental Health, Toxicology and Management of Chemicals (ETM-PERDO), Faculty of Science, Burapha University, Bangsaen, Chonburi 20131, Thailand. Abstract AbstractThe aim of the present study was to standardize and to assess the predictive value of the cytogenetic analysis by Micronucleus (MN) test in fish erythrocytes as a biomarker for marine environmental contamination. Micronucleus frequency Diversity baseline of freshwater in erythrocytes fishes is was spatially evaluated heterogeneous in and genotoxic but, for potentialthe most part, of a commonthe reasons chemical remain poorlywas determined understood inin fishThailand. experimentally This review exposed attempts in toaquarium promulgate under factors controlled linking conditions. freshwater Fishfishes (Therapon and habitats, jaruba particularly) were exposed morphological, for 96 hrsphysiological to a single and heavy ecological, metal (mercuric drawing chloride).on principles Chromosomal and information damage from was elsewhere determined but withas micronuclei the focus being frequency Thailand.
    [Show full text]
  • Habitat Characteristics of the Cyprinidae in Small Rivers in Central Thailand
    Habitat characteristics of the cyprinidae in small rivers in Central Thailand F. William H. Beamish Æ Phannee Sa-ardrit Æ Sumpun Tongnunui Received: 20 October 2005 / Accepted: 15 March 2006 / Published online: 11 May 2006 Ó Springer Science+Business Media B.V. 2006 Abstract Cyprinids were sampled, by electro- relation to environmental factors and ecological fishing, from 159 sites on small rivers in four adaptations. major watersheds across central Thailand. Total abundance, estimated by the depletion method, Keywords Abiotic factors Æ Conservation Æ varied directly with water velocity and, inversely Abundance Æ Occurrence Æ Habitat characteristics Æ with discharge while species richness varied in- Species richness versely with habitat width and, directly with ambient oxygen and alkalinity. Numerical abun- dance of cyprinids was well above that for any of Introduction the other 27 families of fishes represented in the catches. Incidence of occurrence was high only for Habitat provides the medium in which fish may a few cyprinid species in each watershed with fluorish or perish, as well as for the company they most species present in £10% of the sites. Across keep. The importance of habitat has long been all sites, cyprinids represented approximately recognized through studies on environmental 35% of all species captured and were absent from influences on individual species (Beamish 1964a, only two sites. Species richness was highest in the b; Brett 1964, 1979; Albaugh 1972) and assem- Maeklong watershed. Canonical correspondence blages (Felley and Hill 1983; Lobb and Orth 1988; analysis identified five significant habitat vari- Meffe and Sheldon 1988). These and other studies ables, temperature, habitat width, discharge, have identified important environmental vari- ambient oxygen and alkalinity, and the extent to ables for riverine and lake-dwelling fishes, par- which each influenced the distribution of the 41 ticularly in temperate regions of the world (Gee cyprinid species.
    [Show full text]
  • (Actinopterygii: Cyprinidae): DNA Barcoding in Clarifying the Riddle
    The Species Dilemma of Northeast Indian Mahseer (Actinopterygii: Cyprinidae): DNA Barcoding in Clarifying the Riddle Boni A. Laskar, Maloyjo J. Bhattacharjee, Bishal Dhar, Pradosh Mahadani, Shantanu Kundu, Sankar K. Ghosh* Department of Biotechnology, Assam University, Silchar, Assam, India Abstract Background: The taxonomic validity of Northeast Indian endemic Mahseer species, Tor progeneius and Neolissochilus hexastichus, has been argued repeatedly. This is mainly due to disagreements in recognizing the species based on morphological characters. Consequently, both the species have been concealed for many decades. DNA barcoding has become a promising and an independent technique for accurate species level identification. Therefore, utilization of such technique in association with the traditional morphotaxonomic description can resolve the species dilemma of this important group of sport fishes. Methodology/Principal Findings: Altogether, 28 mahseer specimens including paratypes were studied from different locations in Northeast India, and 24 morphometric characters were measured invariably. The Principal Component Analysis with morphometric data revealed five distinct groups of sample that were taxonomically categorized into 4 species, viz., Tor putitora, T. progeneius, Neolissochilus hexagonolepis and N. hexastichus. Analysis with a dataset of 76 DNA barcode sequences of different mahseer species exhibited that the queries of T. putitora and N. hexagonolepis clustered cohesively with the respective conspecific database sequences maintaining 0.8% maximum K2P divergence. The closest congeneric divergence was 3 times higher than the mean conspecific divergence and was considered as barcode gap. The maximum divergence among the samples of T. progeneius and T. putitora was 0.8% that was much below the barcode gap, indicating them being synonymous. The query sequences of N.
    [Show full text]
  • Fish Diversity of North East India, Inclusive of the Himalayan and Indo
    International Journal of Biodiversity and Conservation Vol. 4(15), pp. 592-613, December, 2012 Available online at http://www.academicjournals.org/IJBC DOI: 10.5897/IJBC11.228 ISSN 2141-243X ©2012 Academic Journals Full Length Research Paper Fish diversity of North East India, inclusive of the Himalayan and Indo Burma biodiversity hotspots zones: A checklist on their taxonomic status, economic importance, geographical distribution, present status and prevailing threats Umesh C. Goswami*, Sudip K. Basistha, Dilip Bora, Konthoujam Shyamkumar, Bishnupriya Saikia and Kimneilam Changsan Department of Zoology, Fish Biology and Fishery Sciences, DST-FIST and UGC-SAP Sponsored Department Centre for Aquaculture and Aquatic Resources Monitoring Centre, Biodiversity and Bioinformatics, Gauhati University, Guwahati 781014, Assam, India. Accepted 27 June, 2012 The paper lists 422 fish species from north east India, belonging to 133 genera and 38 families. The maximum diversity is observed in the family Cyprinidae, which is represented by 154 species. Families Anguillidae, Engraulidae, Chacidae, Aplocheilidae, Syngnathidae, Sciaenidae, Osphronemidae, Ophichthidae, Pristigastiridae and Tetraodontidae are represented by a single species each. The habitat, economic importance, distribution (within Northeast India) and threat criteria are listed. The names of the species are corrected after following the new nomenclature. The list includes plain, torrential and estuarine, as well as exotic species. The species are listed from findings of the documented references that have been known till December, 2011. The threats criteria have been finalised from the IUCN & NBFGR reports and physical verification of the habitat, in different seasons and the existing natural and anthropogenic hazards that prevail. It has been found that the fish diversity of these hotspots faces serious threats which would lead to a catastrophic loss of our biodiversity.
    [Show full text]