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Standard portfolio Eurofins Competence Centre

Mono, di- and Test Method Analyte / Component Matrix Technique Code Source Solid food & feed HEC54 Total after Luff Schoorl NEN 3571 Liquid food HEC62 Solid food & feed HEC65 Total reducing sugars (as ) Luff Schoorl In house method Liquid food HEC66 Solid food HEC53 Total reducing sugars (as ) Luff Schoorl In house method Liquid food HEC67 Solid food HEC51 Calculation In house method Liquid food HEC59 Solid food & feed HEC52 Total digestible carbohydrates Luff Schoorl In house method Liquid food HEC60 Sugars in coffee Coffee HEC1M HPAEC-PAD ISO 11292 Food* HEC2M HPAEC-PAD In house method All other matrices HEC2I Food* HEC2L Glucose HPAEC-PAD In house method All other matrices HEC2H (only with PHECB) Food & Feed HEC2F HPAEC-PAD In house method Solid food HEC83 HPAEC-PAD In house method Liquid food HEC84 Food (exc. dairy)* HEC2N Dairy* HEC36 HPAEC-PAD In house method Lactose All other matrices HEC2J Liquid food HEC61 Luff Schoorl In house method Lactose free Solid & liquid food HEC79 HPAEC-PAD In house method Food* HEC2P HPAEC-PAD In house method All other matrices HEC2G Food* HEC2Q HPAEC-PAD In house method All other matrices HEC2K Food HEC0C HPAEC-PAD In house method Solid food HEC49 HPAEC-PAD In house method Liquid food HEC50 Palatinose () Solid & liquid food HEC0U HPAEC-PAD In house method Kestose (GF2 from ) Solid & liquid food HEC0S HPAEC-PAD In house method Nystose (GF3 from inulin) Solid & liquid food HEC0T HPAEC-PAD In house method //Verbascose Solid & liquid food HEC1K HPAEC-PAD In house method Malto-oligosaccharides (DP1-DP7) Solid & liquid food HEC0I HPAEC-PAD In house method Ingredients Fructose oligomers (F2-F6) quant. For other matrices HEC0R HPAEC-PAD In house method contact us * : accredited test

Starch and Dietary Fibre (incl. prebiotics) Test Analyte / Component Matrix Technique Method Source Code Solid & liquid food* HEC57 ISO 15914 Feed* HEC81 total Enzymatic Meat & fatty HEC1R In house method products Starch (incl. ) Solid & liquid food HEC0L Enzymatic In house method Damaged Starch Food HEC42 Enzymatic AACC 76-31 Gelatinized Starch Feed HEC2T Enzymatic AACC 76-31 Resistant Starch Solid food & Feed HEC87 Enzymatic AOAC 2002.02 Food & Feed with & in starch HEC0J Titration In house method >10% starch Amylose & amylopectin in sample Food & Feed with HEC2X Calculation In house method (only with PHE02) >10% starch Starch/ and Feed HEC2R HPAEC-PAD / In house method Carbohydrate composition of sol. Colorimetric (contact us for more & insol. NSP excl. info) Solid food HEC56 Classical dietary fibres, total Liquid food HEC63 Gravimetric AOAC 985.29

Breakfast cereals* HEC55 Classical total dietary fibres Solid & liquid food HEC1Q Gravimetric AOAC 991.43 (TDF / IDF / SDF) Total dietary fibres (HMWDF Enzym./Gravim. Solid & liquid food HEC1A AOAC 2009.01 (incl. RS) / LMWDF) GPC-RI Total dietary fibres (HMWDF Food with high Enzym./Gravim. AOAC 2009.01 HEC1F (incl. RS) / LMWDF) starch content GPC-RI (revised)

Total dietary fibres (IHMWDF Enzym./Gravim. Solid & liquid food HEC1B AOAC 2011.25 (incl. RS) / SHMWDF / LMWDF) GPC-RI Ingredients with - (yeast & moulds) HEC1J Enzymatic In house method >40% -glucan Solid & liquid food -glucan (cereals) HEC1C Enzymatic AOAC 995.16 high Glc content Solid & liquid food -glucan (cereals) HEC2Z Enzymatic AOAC 995.16 Low Glc content (as total uronic acid) Solid & liquid food HEC2D Colorimetric In house method Total sialic acid HEC2W HPAEC-PAD In house method Solid food HEC26 (>10% lactose) Galacto-oligosaccharides Enzymatic / Solid food, GOS (GOS) HPAEC-PAD AOAC 2001.02 ingredients & HEC1H premixes (<10% lactose) * : accredited test

Starch and Dietary Fibre (incl. prebiotics) cont. Test Method Analyte / Component Matrix Technique Code Source All liquid samples Galacto-oligosaccharides (incl. syrups), GOS Enzymatic / HEC1I AOAC 2001.02 (GOS) ingredients and HPAEC-PAD premixes Degree of polymerization of GOS contact GPC-RI / Ingredients In house method (DP2-DP8) us HPAEC-PAD Dietary fibre containing contact Enzymatic / GPC- Solid & Liquid food AOAC 2001.03 supplemented res. maltodextrin us RI Ingredients HEC1G AOAC 997.08 Inulin / Fructo-oligosaccharides Enzymatic / Solid food HEC3D (FOS) HPAEC-PAD AOAC 999.03 Liquid food HEC3E Solid food HEC39 Enzymatic / Polydextrose AOAC 2000.11 Liquid food HEC38 HPAEC-PAD Solid food HEC71 (only in combination with red. Calculation In house method sugars and moisture tests) Liquid food HEC72

Fingerprint (qualitative tests) Test Method Analyte / Component Matrix Technique Code Source Ingredients Starch hydrolysate For other matrices HEC0N HPAEC-PAD In house method (maltodextrin) contact us Solid ingredients HEC30 Inulin & Fructo-oligosaccharides HPAEC-PAD In house method Liquid ingredients HEC31 Galacto-oligosaccharides Ingredients HEC0P HPAEC-PAD In house method For other matrices Polydextrose contact us HEC0Q HPAEC-PAD In house method

Sweeteners Test Method Analyte / Component Matrix Technique Code Source Solid & liquid food HEC85 HPAEC-PAD In house method Ingredients & HEG53 solid food HPAEC-PAD In house method Liquid food HEC74

Carbohydrate derivatives Test Method Analyte / Component Matrix Technique Code Source Total uronic acid Solid & liquid food HEC2D Colorimetric In house method contact Galacturonic acid Solid & liquid food HPAEC-PAD In house method us contact Glucuronic acid Solid & liquid food HPAEC-PAD In house method us

Packages Test Method Analyte / Component Matrix Technique Code Source Degree of gelatinization Solid food PHECA Calculation In house method (calc. damaged starch / starch) Pet food profile (Glc, Fru, Lac, Suc, Mal All matrices PHECB HPAEC-PAD In house method + Gal) Total sugar incl. Gal (to order only All matrices HEC3A Calculation together with PHECB) Sugar profile (Glc, Fru, Lac, Suc, Food* PHECC HPAEC-PAD In house method Mal) Total sugar (to order only in Food HEC3B Calculation combination with PHECC) Sugar profile (Glc, Fru, Lac, Suc, All matrices (except PHECD HPAEC-PAD In house method Mal) food) Total sugar (to order only in All matrices (except HEC3C Calculation combination with PHECD) food) Amylose / starch / amylopectin Starch & cereals PHE02 In house method * : accredited test

Nutritional Value Packages for products supplemented with low molecular weight dietary fibre (prebiotics, polydextrose, res. maltodextin etc.) Test Method Analyte / Component Matrix Technique Code Source Nutritional value model II incl. Solid food PHEP9 In house method Dietary Fibre AOAC 2009.1 Nutritional value model II incl. Solid sugary products PHEPA In house method Dietary Fibre AOAC 2009.1 Nutritional value model II incl. Liquid food PHEPB In house method Dietary Fibre AOAC 2009.1 Nutritional value model II incl. Liquid sugary PHEPC In house method Dietary Fibre AOAC 2009.1 products Nutritional value model II incl. Dietary supplements PHEP5 In house method Dietary Fibre AOAC 2009.1

Decision tree for dietary fibre determination

Contact: Eurofins Carbohydrate Competence Centre Leeuwarderstraatweg 129 8441 PK Heerenveen The Netherlands Tel. +31 88 8310000 Fax +31 88 8310010 Mail: [email protected] Web: www.eurofins.nl Web: www.carbohydratestesting.com version 01/2017