Probing the Nutritive and Therapeutic Potential of Pakistani Sesame Cultivars for School Going Children
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PROBING THE NUTRITIVE AND THERAPEUTIC POTENTIAL OF PAKISTANI SESAME CULTIVARS FOR SCHOOL GOING CHILDREN By Sabiha Abbas 2006-ag-1393 M.Sc. (Hons.) Food Technology A thesis submitted in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY IN FOOD TECHNOLOGY NATIONAL INSTITUTE OF FOOD SCIENCE & TECHNOLOGY FACULTY OF FOOD, NUTRITION AND HOME SCIENCES UNIVERSITY OF AGRICULTURE FAISALABAD PAKISTAN 2019 i ii iii iv v DEDICATION I dedicate this humble effort to HOLY PROPHET HAZRAT MUHAMMAD (PEACE BE UPON HIM) And My Father, Mother (Late), sisters and brothers, who never stop believing in me vi ACKNOWLEDGEMENTS I am proud to think that what words do justice to express my thanks to ALMIGHT ALLAH (The Omnipotent, The Omniscent, The Most Merciful and The Most Beneficient) who is the entire source of all knowleddge and wisdom to mankind. His bounteous blessing that flourished my thoughts and fulfilled my ambitions and my modest efforts in the form of this write up and made this material contribution towards the deep ocean of scientific knowledge already existing and which is a permanent source of benefit for His humanity and creatures. All praises be to the HOLY PROPHET MUHAMMAD (Peace be Upon Him), the city of knowledge, the illuminating torch and the rescuer of humanity from giving astray, Who’s teachings enlightened my heart and flourished my thoughts. The work presented in this manuscript was impossible to be accomplished without the sympathetic attitude, utmost care, observant pursuit, scholarly criticism, cheering perspective and enlightened supervision of my sweet teacher Dr. Mian Kamran Sharif (Assiociate Professor), National Institute of Food Science and Technology, University of Agriculture, Faisalabad. I am grateful to his ever-inspiring guidance and constructive suggestions throughout the course of this effort. I am thankful to the member of my supervisory committee, Prof. Dr. Masood Sadiq Butt, Dean, Faculty of Food, Nutrition and Home Sciences, University of Agriculture, Faisalabad, Prof. Dr. Nuzhat Huma, Director Geneal, National Institue of Food Science and Technology, University of Agriculture, Faisalabad Dr. Muhammad Shahid (Associate Professor), Department of Biochemistry, University of Agriculture, Faisalabad, for their consistent guidance to accomplish this study. Words are lacking to express my humble obligation to my “Sweet Father, Mother (Late), Sisters and Brothers” who always longed for my successful and happy life. Their endless efforts and best wishes sustained me at all stages of my life and encouraged me for achieving high ideas of life and their hands always remain raised in prayer for my success. O ALLAH! Shower your countless blessing on them. It’s my ever pray that Allah bless them with long, happy and healthy life. Extreme love, utmost sincerity and caring behavior of my senior fellows Dr. Faiz ul Hassan Shah, my junior fellows Mr. Ghulam Murtaza, Mr. Wahab Ali Khan and my sweet sister and friends Ms.Shamaila Abbas, Ms.Sana Sadaat, Ms. Farah Ahmad, Ms. Mehwish Liaquat. The sacrifices, they offered for me, will always shine in my mind like twinkling stars. O ALLAH! Bless them with all the happiness of life and highest status in the next coming permanent world. If pearls were words and flowers feelings, it would be easier to express my heart felt gratitude and indebtedness to all the PEOPLES and FRIENDS who HELPED me in any way throughout my life. May Allah Almighty infuse me with the energy to fulfill their noble inspiration and expectations and further modify my competence. May Allah bless us all with long, happy and peaceful lives. Sabiha Abbas vii viii 4.5 Biological Evaluation of Sesame Flour Supplemented 107 Doughnuts 4.6 Efficacy Studies in school aged children 113 5 Summary 160 Recommendations and future directions 165 Limitations of the study 166 Literature Cited 167 Annexures 188 ix List of Tables Sr. No. Contents Page # 3.1 Formulations used in study (g/100g) 40 3.2 Experimental diets 44 Mean squares for proximate composition of sesame flours of 4.1 49 different cultivars Proximate composition (%) of sesame flours of different 4.2 50 cultivars Mean squares for mineral contents of sesame flours of different 4.3 53 cultivars Mineral contents (mg/100g) of sesame flours of different 4.4 54 cultivars Mean squares for essential amino acids in sesame flours of 4.5 57 different cultivars Mean squares for non-essential amino acids in sesame flours of 4.6 58 different cultivars Essential amino acids composition (g/100g) of sesame flours of 4.7 59 different cultivars Non-essential amino acids composition (g/100g) of sesame 4.8 61 flours of different cultivars Mean squares for antioxidant potential of sesame flours of 4.9 64 different cultivars 4.10 Antioxidant potential of sesame flours of different cultivars 65 Mean squares for bioactive components in sesame flours of 4.11 68 different cultivars Bioactive components (ppm) in sesame flours of different 4.12 69 cultivars Mean squares for sensory evaluation of white and black sesame 4.13 71 flour supplemented doughnuts Effect of white and black sesame flour supplementation on the 4.14 72 color of doughnuts Effect of white and black sesame flour supplementation on the 4.15 74 aroma of doughnuts Effect of white and black sesame flour supplementation on the 4.16 75 taste of doughnuts Effect of white and black sesame flour supplementation on the 4.17 77 texture of doughnuts Effect of white and black sesame flour supplementation on the 4.18 78 chewability of doughnuts x 4.19 Effect of white and black sesame flour supplementation on the 80 mouthfeel of doughnuts Effect of white and black sesame flour supplementation on the 4.20 81 overall acceptability of doughnuts Mean squares for proximate composition of sesame flour 4.21 85 supplemented doughnuts Effect of treatments on the proximate composition (%) of 4.22 86 sesame flour supplemented doughnuts Mean squares for mineral contents of sesame flour 4.23 89 supplemented doughnuts Effect of treatments on the mineral contents (mg/100g) of 4.24 90 sesame flour supplemented doughnuts Mean squares for texture analysis of sesame flour supplemented 4.25 93 doughnuts Effect of treatments on the textural characteristics of sesame 4.26 95 flour supplemented doughnuts Mean squares for color characteristics and calorific value of 4.27 97 sesame flour supplemented doughnuts Effect of treatments on the color and calorific value of sesame 4.28 98 flour supplemented doughnuts Mean squares for the water activity, peroxide value, 4.29 thiobarbituric acid number and growth of sesame flour 100 supplemented doughnuts Effect of treatments and storage on the water activity of sesame 4.30 101 flour supplemented doughnuts Effect of treatments and storage on peroxide value (meq/Kg) of 4.31 103 sesame flour supplemented doughnuts Effect of treatments and storage on thiobarbituric acid number 4.32 105 (mg/kg) of sesame flour supplemented doughnuts Effect of treatments and storage on the mold count (×102 CFU/g) 4.33 106 of sesame flour supplemented doughnuts 4.34 Mean squares for PER and NPR of experimental diets 108 Effect of experimental diets on PER and NPR in Sprague Dawly 4.35 109 rats 4.36 Mean squares for TD, BV and NPU of experimental diets 111 Effect of experimental diets on the TD, BV and NPU in Sprague 4.37 112 Dawly rats Mean daily dietary intake of study groups (n=45) through 24-h 4.38 114 recall method xi 4.39 Mean squares for the anthropometric measurements of school 116 aged children 4.40 Effect of protein enriched sesame fortified doughnuts on height 117 of school aged children 4.41 Effect of protein enriched sesame fortified doughnuts on weight 119 of school aged children 4.42 Effect of protein enriched sesame fortified doughnuts on body 120 mass index (BMI) of school aged children 4.43 Effect of protein enriched sesame fortified doughnuts on head 122 circumference (HC) of school aged children 4.44 Effect of protein enriched sesame fortified doughnuts on mid 123 upper arm circumference (MUAC) of school aged children 4.45 Mean squares for the serum ferritin and serum zinc levels of 124 school aged children 4.46 Effect of protein enriched sesame fortified doughnuts on serum 126 ferritin of school aged children 4.47 Effect of protein enriched sesame fortified doughnuts on serum 128 zinc of school aged children 4.48 Mean squares for complete blood count (CBC) of school aged 132 children 4.49 Effect of protein enriched sesame fortified doughnuts on 133 hemoglobin (Hb) of school aged children 4.50 Effect of protein enriched sesame fortified doughnuts on red 135 blood cells (RBCs) of school aged children 4.51 Effect of protein enriched sesame fortified doughnuts on packed 138 cell volume (PCV) of school aged children 4.52 Effect of protein enriched sesame fortified doughnuts on mean 140 corpuscular volume (MCV) of school aged children 4.53 Effect of protein enriched sesame fortified doughnuts on mean 143 corpuscular hemoglobin (MCH) of school aged children 4.54 Effect of protein enriched sesame fortified doughnuts on mean 145 corpuscular hemoglobin concentration (MCHC) of school aged children 4.55 Effect of protein enriched sesame fortified doughnuts on 148 platelets and white blood cells (WBCs) of school aged children 4.56 Effect of protein enriched sesame fortified doughnuts on 149 neutrophils and lymphocytes of school aged children xii 4.57 Effect of protein enriched sesame fortified doughnuts on 151 monocytes and eosinophils of school aged children 4.58 Mean squares for serum electrolytes of school aged children 152 4.59 Effect of protein enriched sesame fortified doughnuts on blood 153 sodium, chloride and potassium of school aged children 4.60 Mean squares for the renal function test of school aged children 155 4.61 Effect of protein enriched sesame fortified doughnuts on blood 156 urea and serum creatinine of school aged children 4.62 Mean squares for liver function test of school aged children 158 4.63 Effect of protein enriched sesame fortified doughnuts on 159 bilirubin (total, conjugated and unconjugated) of school aged children xiii List of Figures Sr.