Histone H3 Rabbit Pab

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Leader in Biomolecular Solutions for Life Science Histone H3 Rabbit pAb Catalog No.: A7010 Basic Information Background Catalog No. Histones are basic nuclear proteins that are responsible for the nucleosome structure of A7010 the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is Observed MW wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with 15kDa linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent Calculated MW histone that is a member of the histone H3 family. Transcripts from this gene lack polyA 15kDa tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. Category Primary antibody Applications WB, IHC, IF Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 8351 P68431 IHC 1:50 - 1:200 Immunogen 1:50 - 1:100 IF Recombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human HIST1H3D (NP_003520.1). Synonyms HIST1H3D;H3/b;H3FB;histone H3.1;Histone H3;HIST1H3A Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using HIST1H3D antibody (A7010) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s. Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- embedded rat spleen using Histone H3 embedded human kidney cancer using embedded mouse cancer using Histone H3 antibody (A7010) at dilution of 1:100 (40x Histone H3 antibody (A7010) at dilution of antibody (A7010) at dilution of 1:100 (40x lens). 1:100 (40x lens). lens). Immunofluorescence analysis of MCF7 cells using Histone H3 antibody (A7010). Blue: DAPI for nuclear staining. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..

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HIST1H3D: a Promising Therapeutic Target for Lung Cancer
INTERNATIONAL JOURNAL OF ONCOLOGY 50: 815-822, 2017 HIST1H3D: A promising therapeutic target for lung cancer YAN RUI1*, WEN-JIA PENG2*, MING WANG1*, QIAN WANG1*, ZI-LI LIU1, YU-QING CHEN1 and LI-NIAN HUANG1 1Department of Respiration and Critical Care Medicine, The First Affiliated Hospital of Bengbu Medical College, Lung Cancer Diagnosis and Treatment Center of Anhui Province, Anhui Provincial Key Laboratory of Clinical Basic Research on Respiratory Disease, Bengbu, Anhui 233004; 2Department of Epidemiology and Health Statistics, Bengbu Medical College, Bengbu, Anhui 233000, P.R. China Received October 6, 2016; Accepted December 1, 2016 DOI: 10.3892/ijo.2017.3856 Abstract. HIST1H3D gene encodes histone H3.1 and is involved CDKN1 and CCNE2 genes. In conclusion, our results suggest in gene-silencing and heterochromatin formation. HIST1H3D that HIST1H3D is highly expressed in lung cancer cell lines expression is upregulated in primary gastric cancer tissue. In and tissues. Furthermore, HIST1H3D may be important in this study, we explored the effects of HIST1H3D expression cell proliferation, apoptosis and cell cycle progression, and is on lung cancer, and its mechanisms. HIST1H3D expression implicated as a potential therapeutic target for lung cancer. was measured by immunohistochemistry and RT-PCR in lung cancer tissues and human lung cancer cell lines. Cell prolif- Introduction eration was assessed by MTT assay. Flow cytometric analysis was used to determine cell cycle distribution and apoptosis. Lung cancer is one of the most common cancers and the Levels of related proteins were detected by western blotting. major cause of cancer deaths worldwide, with 1.6 million new Bioinformatics analysis was performed to investigate related lung cancer cases and 1.4 million lung cancer deaths each signaling pathways.
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Contrasting Expression Patterns of Histone Mrna and Microrna 760 in Patients with Gastric Cancer
Published OnlineFirst October 4, 2013; DOI: 10.1158/1078-0432.CCR-12-3186 Clinical Cancer Human Cancer Biology Research Contrasting Expression Patterns of Histone mRNA and microRNA 760 in Patients with Gastric Cancer Takeshi Iwaya1,2, Takeo Fukagawa3, Yutaka Suzuki4, Yusuke Takahashi1, Genta Sawada1, Masahisa Ishibashi1, Junji Kurashige1, Tomoya Sudo1, Fumiaki Tanaka1, Kohei Shibata1, Fumitaka Endo2, Hirokatsu Katagiri2, Kaoru Ishida2, Kohei Kume2, Satoshi Nishizuka2, Hisae Iinuma5, Go Wakabayashi2, Masaki Mori6, Mitsuru Sasako7, and Koshi Mimori1 Abstract Purpose: Recent studies revealed that both disseminated tumor cells and noncancerous cells contributed to cancer progression cooperatively in the bone marrow. Here, RNA-seq analysis of bone marrow from gastric cancer patients was performed to identify prognostic markers for gastric cancer. Experimental Design: Bone marrow samples from eight gastric cancer patients (stages I and IV: n ¼ 4 each) were used for RNA-seq analysis. Results were validated through quantitative real-time PCR (qRT-PCR) analysis of HIST1H3D expression in 175 bone marrow, 92 peripheral blood, and 115 primary tumor samples from gastric cancer patients. miR-760 expression was assayed using qRT-PCR in 105 bone marrow and 96 primary tumor samples. Luciferase reporter assays were performed to conﬁrm whether histone mRNAs were direct targets of miR-760. miR-760 expression was also evaluated in noncancerous cells from gastric cancer patients. Results: RNA-seq analysis of bone marrow samples from gastric cancer patients revealed higher expression of multiple histone mRNAs in stage IV patients. HIST1H3D expression in the bone marrow, peripheral blood, and primary tumor of stage IV patients was higher than that in stage I patients (P ¼ 0.0284, 0.0243, and 0.0006, respectively).
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Genome-Wide Screen of Cell-Cycle Regulators in Normal and Tumor Cells
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Characterizing Novel Interactions of Transcriptional Repressor Proteins BCL6 & BCL6B
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Supplemental Data.Pdf
Supplementary material -Table of content Supplementary Figures (Fig 1- Fig 6) Supplementary Tables (1-13) Lists of genes belonging to distinct biological processes identified by GREAT analyses to be significantly enriched with UBTF1/2-bound genes Supplementary Table 14 List of the common UBTF1/2 bound genes within +/- 2kb of their TSSs in NIH3T3 and HMECs. Supplementary Table 15 List of gene identified by microarray expression analysis to be differentially regulated following UBTF1/2 knockdown by siRNA Supplementary Table 16 List of UBTF1/2 binding regions overlapping with histone genes in NIH3T3 cells Supplementary Table 17 List of UBTF1/2 binding regions overlapping with histone genes in HMEC Supplementary Table 18 Sequences of short interfering RNA oligonucleotides Supplementary Table 19 qPCR primer sequences for qChIP experiments Supplementary Table 20 qPCR primer sequences for reverse transcription-qPCR Supplementary Table 21 Sequences of primers used in CHART-PCR Supplementary Methods Supplementary Fig 1. (A) ChIP-seq analysis of UBTF1/2 and Pol I (POLR1A) binding across mouse rDNA. UBTF1/2 is enriched at the enhancer and promoter regions and along the entire transcribed portions of rDNA with little if any enrichment in the intergenic spacer (IGS), which separates the rDNA repeats. This enrichment coincides with the distribution of the largest subunit of Pol I (POLR1A) across the rDNA. All sequencing reads were mapped to the published complete sequence of the mouse rDNA repeat (Gene bank accession number: BK000964). The graph represents the frequency of ribosomal sequences enriched in UBTF1/2 and Pol I-ChIPed DNA expressed as fold change over those of input genomic DNA.
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Robles JTO Supplemental Digital Content 1
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