Tumor Destruction and Kinetics of Tumor Cell Death in Two Experimental Mouse Tumors Following Photodynamic Therapy1
[CANCER RESEARCH 45, 572-576, February 1985] Tumor Destruction and Kinetics of Tumor Cell Death in Two Experimental Mouse Tumors following Photodynamic Therapy1 Barbara W. Henderson,2 Stephen M. Waldow, Thomas S. Mang, William R. Potter, Patrick B. Malone, and Thomas J. Dougherty Division ot Radiation Biology, Department of Radiation Medicine, Roswell Park Memorial Institute, Buffalo, New York 14263 ABSTRACT rapid tumor necrosis (8, 11). Preceding tumor necrosis are pronounced changes in the vascular system of the tumor (3, 5, The effect of photodynamic therapy (PDT) on tumor growth 23). as well as on tumor Å“il survival in vitro and in vivo was studied In vitro, dose-dependent (photosensitizer + light) photody in the EMT-6 and RIF experimental mouse tumor systems. In namic cell killing is characterized by cell lysis within 1 hr after a vitro, RIF cells were more sensitive towards PDT than were lethal PDT dose (1) and can be achieved in all cell types studied EMT-6 cells when incubated with porphyrin (25 u,g/m\, dihema- thus far, normal as well as malignant (2, 6,14). toporphyrin ether) and subsequently given graded doses of light. Despite extensive in vivo and in vitro studies, it has not yet In vivo, both tumor types responded to PDT (EMT-6, dihemato- been established whether tumor cell killing in vivo is governed porphyrin ether, 7.5 mg/kg; RIF, dihematoporphyrin ether, 10 by the same mechanism as is cell killing in vitro. To answer this mg/kg; both followed 24 hr later by 135 J of light at 630 nm/sq question, we studied the relationship between tumor destruction, cm) with severe vascular disruption and subsequent disappear tumor cure, and tumor cell survival kinetics in 2 experimental ance of tumor bulk.
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