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Home , Anhui University, Shandong University

KE ZHANG, PH.D.

Department of Chemistry and Chemical Biology Northeastern University 360 Huntington Ave, Boston 02115 Tel: (617) 373-4415 | Email: [email protected] Group website: www.northeastern.edu/polymer Google Scholar: https://scholar.google.com/citations?user=whWec_kAAAAJ&hl=en

EDUCATION University of Technology 2001-2005 B.S. in Applied Chemistry

Washington University in St. Louis 2005-2009 Ph.D. in Chemistry Advisor: Prof. Karen Wooley

Northwestern University 2010-2012 Postdoctoral fellow in the Department of Chemistry Advisor: Prof. Chad Mirkin

EMPLOYMENT Associate Professor Northeastern University 2017-present Department of Chemistry and Chemical Biology

Assistant Professor Northeastern University 2012-2017 Department of Chemistry and Chemical Biology

HONORS AND AWARDS ACS PMSE Young Investigator Award 2018 Nano Research Young Innovator Award 2017 NSF CAREER Award 2014 ACS PRF Doctoral New Investigator Award 2014 International Institute of Nanotechnology Outstanding Researcher Award 2012

PUBLICATIONS Peer-reviewed journal articles At Northeastern: Bolded names: undergraduate researchers 1. Tan, X.; Jia, F.; Miller, A. E.; Zhang, K.* “Nucleic acid-based drug delivery strategies”, submitted 2. Li, H.; Li, Y.; Xiao, Y.; Zhang, B.; Cheng, Z.; Shi, J.; Xiong, J.; Li, Z.; Zhang, K.* “Molecularly well-defined DNA-polymer miktoarm stars for enzyme-resistant nanoflares and carrier-free gene regulation”, Bioconjugate Chemistry, 2020, DOI: 10.1021/acs.bioconjchem.0c00017

1 3. Jia, F.; Song, J.; Kubiak, J.; Santos, P.; Sano, K.; Holten-Andersen, N.; Zhang, K.; Macfarlane, R. “Bottlebrush polymers as nanoscale building blocks for hydrogel synthesis”, submitted 4. Qin, X.; Si, Y.; Wu, Z.; Zhang, K.; Li, J.; Yin, Y. “Alkyne/Ruthenium(II) complex-based ratiometric SERS nanoprobe for in vitro and ex vivo tracking of carbon monoxide”, Analytical Chemistry, 2020, 92, 924 5. Yin, S.; Wang, N.; Liu, W.; Zhang, K.; Mirkin, C. A.; Li, J. “A metal oxide-MOF composite nanoenzyme for light-free photodynamic therapy”, submitted 6. Shi, X.; Meng, H.; Chen, J.; Gao, Y.; Qu, L.; Zhang, K.; Zhang, X.; Li, Z. “DNA amplifier- functionalized metal-organic frameworks for multiplexed detection and imaging of intracellular mRNA”, ACS Sensors, 2020, DOI: 10.1021/acssensors.9b01759 7. Wu, Y.; Meng, H.; Chen, J.; Jiang, K.; Yang, R.; Li, Y.; Tang, J.; Zhang, K.; Qu, L.; Li, Z. “Accelerated DNAzyme-based fluorescent nanoprobe for highly sensitive microRNA imaging in live cells”, Chemical Communications, 2020, 56, 470 8. Yin, X.; Sun, Y.; Geng, X.; Li, J.; Yang, R.; Zhang, K.; Qu, L.; Li, Z. “Spatiotemporally monitoring cell viability through programmable mitochondrial membrane potential transformation by using fluorescent carbon dots”, Advanced Biosystems, 2020, DOI: doi.org/10.1002/adbi.201900261 9. Xie, Y.; Wang, N.; Li, Y.; Li, J.; Zhang, K.; Yu, R. “Cyclodextrin supramolecular inclusion-enhanced pyrene excimer switching for highly selective detection of RNase H”, Analytica Chimica Acta, 2019, DOI: j.aca.2019.08.059 10. Xiao, Y.; Li, Y.; Zhang, B.; Li, H.; Cheng, Z.; Shi, J.; Xiong, J.; Bai, Y.; Zhang, K.* “Functionalizable, side chain-immolative poly(benzyl ether)s”, ACS Macro Letters, 2019, 8, 399 11. Jia, F.; Li, H.; Chen, R.; Zhang, K.* “Self-assembly of DNA-containing copolymers”, Bioconjugate Chemistry, 2019, DOI: 10.1021/acs.bioconjchem.9b00067 12. Wang, D.; Lin, J.; Jia, F.; Tan, X.; Wang, Y.; Sun, X.; Cao, X.; Che, F.; , H.; Gao, X.; Shimkonis, J. C.; Nyoni, Z.; Lu, X.; Zhang, K.* “Bottlebrush-architectured poly(ethylene glycol) as an efficient vector for RNA interference in vivo”, Science Advances, 2019, DOI: 10.1126/sciadv.aav9322 (highlighted in Northeastern News and ScienceTrends) 13. Tan, X.; Lu, H.; Sun, Y.; Chen, X.; Wang, D.; Jia, F.; Zhang, K.* “Expanding the materials space of DNA via organic-phase ring-opening metathesis polymerization”, Chem, 2019, DOI: 10.1016/j.chempr.2019.03.023 14. Gudipati, S.; Zhang, K.; Rouge, J. L. “Towards self-transfecting nucleic acid nanostructures for gene regulation”, Trends in Biotechnology, 2019, DOI: 10.1016/j.tibtech.2019.01.008 (cover article) 15. Wang, N.; Song, L.; Xing, H.; Zhang, K.; Li, J. “Spherical nucleic acid-based two-photon nanoprobe for RNase H activity assay in living cells and tissues”, Nanoscale, 2019, DOI: 10.1039/C9NR00880B 16. Li, M.; Wang, C.; Di, Z.; Li, H.; Zhang, J.; Xue, W.; Zhao, M.; Zhang, K.; Zhao, Y.; Li, L. “ multifunctional DNA hybrid nanospheres through coordination-driven self- assembly”, Angewandte Chemie International Edition, 2018, DOI: 10.1002/anie.201810735

2 17. Jia, F.; Wang, D.; Lu, X.; Tan, X.; Wang, Y.; Lu, H.; Zhang, K.* “Improving the enzymatic stability and pharmacokinetics of oligonucleotides via DNA-backboned brush polymers”, Nano Letters, 2018, 18, 7378 18. Lu, X.; Zhang, K.* “PEGylation of therapeutic oligonucleotides – From linear to highly branched PEG architectures”, Nano Research, 2018, DOI: 10.1007/s12274-018-2131-8 (invited article for the 45 under 45 Young Innovator Award special issue) 19. Xiao, Y.; Li, H.; Zhang, B.; Cheng, Z.; Li, Y.; Tan, X.; Zhang, K.* “Modulating the depolymerization of self-immolative brush polymers with poly(benzyl ether) backbones”, Macromolecules, 2018, 51, 2899 20. Li, H.; Zhang, B.; Lu, X.; Tan, X.; Jia, F.; Xiao, Y.; Cheng, Z.; Li, Y.; Zhang, K.*; Mirkin, C. A. “Molecular spherical nucleic acids”, Proceedings of the National Academy of Sciences, USA, 2018, 115, 4340-4344 21. Tan, X.; Burchfield, E.; Zhang, K.* “Light-responsive drug delivery systems” in Stimuli- Responsive and Trigger Drug Delivery Systems, Edited by Singh, A. and Amiji, M., RSC Publishing, London, UK, 2018, 163-191 22. Tan, X.; Zhang, K.* “Photolabile self-immolative DNA-drug nanostructures” in Biomedical Nanotechnology: Methods and Protocols 2nd Edition, Edited by Hurst, S. J., Springer, New York, USA, 2017, 209-221 23. Cressiot, B.; Greive, S. J.; Si, W.; Pascoa, T.; Mojtabavi, M.; Chechik, M.; Jenkins, H. T.; Lu, X.; Zhang, K.; Aksimentiev, A.; Antson, A. A.; Wanunu, M. “Porphyrin-assisted docking of a thermophage portal protein into lipid bilayers: nanopore engineering and characterization”; ACS Nano, 2017, 11, 11931-11945 24. Wang, D.; Lu, X.; Jia, F.; Tan, X.; Xiaoya, S.; Cao, X.; Wai, F.; Zhang, K.* “Precision tuning of DNA- and poly(ethylene glycol)-based nanoparticles via co-assembly for effective antisense gene regulation”, Chemistry of Materials, 2017, 29, 9882-9886 25. Li, Y.; Sun, S.; Fan, L.; Hu, S.; Huang, Y.; K. Zhang.; Nie, Z.; Yao, S. “Peptide logic circuits based on chemoenzymatic ligation for programmable cell apoptosis”, Angewandte Chemie International Edition, 2017, 56, 14888-14892 26. Antczak, N. M.; Packer, M. R.; Lu, X.; Zhang, K.; Beuning, P. J. “Human Y-family DNA polymerase kappa is more tolerant to changes in its active site loop than its ortholog E. coli DinB”, Chemical Research in Toxicology, 2017, doi: 10.1021/acs.chemrestox.7b00175 27. Wang, N.; Yu, X.; Zhang, K.*; Mirkin, C. A.; Li, J. “Upconversion nanoprobes for the ratiometric luminescent sensing of nitric oxide”, Journal of the American Chemical Society, 2017, 139, 12354-12357 28. Cao, X.; Lu, X.; Wang, D.; Jia, F.; Tan, X.; Corley, M.; Chen, X.; Zhang, K.* “Modulating the cellular immune response of oligonucleotides by brush polymer-assisted compaction”, Small, 2017, doi: 10.1002/smll.201701432 29. Jia F.; Lu, X.; Wang, D.; Cao, X.; Tan, X.; Zhang, K.* “Depth-profiling the nuclease stability and the gene silencing efficacy of brush-architectured poly(ethylene glycol)-DNA conjugates” Journal of the American Chemical Society, 2017, 139, 10605-10608 30. Jia, F.; Lu, X.; Tan, X.; Wang, D.; Zhang, K.* “Effect of PEG architecture on the hybridization thermodynamics and protein accessibility of PEGylated oligonucleotides”, Angewandte Chemie International Edition, 2017, 56, 1239-1243

3 31. Lu, X.; Jia, F.; Tan, X.; Wang, D.; Cao, X.; Zheng, J.; Zhang, K.* “Effective antisense gene regulation via non-cationic, polyethylene glycol brushes”, Journal of the American Chemical Society, 2016, 138, 9097-9100 32. Tan, X.; Lu, X.; Jia, F.; Liu, X.; Logan, J. K.; Sun, Y.; Zhang, K.* “Blurring the role of oligonucleotides: Spherical nucleic acids as a drug delivery vehicle”, Journal of the American Chemical Society, 2016, 138, 10834-10837 33. Lu, X.; Tran, T.-H.; Jia, F.; Tan, X.; Davis, S.; Amiji, M.; Zhang, K.* “Providing oligonucleotides with steric selectivity by brush polymer-assisted compaction”, Journal of the American Chemical Society, 2015, 137, 12466-12469 (selected for JACS Young Investigator virtual issue) 34. Tan, Xu; Li, B. B.; Lu, X.; Jia, F.; Santori, C.; Menon, P.; Li, H.; Zhang, B.; Zhao, J. J.; Zhang, K.* “Light-triggered, self-immolative nucleic acid-drug nanostructures”, Journal of the American Chemical Society, 2015, 137, 6112-6115 35. Chen, Y.; Zhang, K.; Wang, X.; Zhang, F.; Zhu, J.; Mays, J.; Wooley, K. L.; Pochan, D., “Multigeometry nanoparticles: Hybrid vesicle/cylinder nanoparticles constructed with block copolymer solution assembly and kinetic control”, Macromolecules, 2015, 48, 5621- 5631 36. Nevin, P.; Lu, X.; Zhang, K.; Engen, J. R.; Beuning, P. J. “Non-cognate DNA damage prevents formation of active conformation of Y-family polymerases DinB and Pol kappa”, FEBS, 2015, 29, 561.8 37. Jia, F.; Lu, X.; Tan, X.; Zhang, K.* “Facile synthesis of nucleic acid-polymer amphiphiles and their self-assembly”, Chemical Communications, 2015, 51, 7843-7846 38. Lu, X.; Watts, E.; Jia, F.; Tan, X.; Zhang, K.* “Polycondensation of polymer brushes via DNA hybridization”, Journal of the American Chemical Society, 2014, 136, 10214-10217 39. Zhang, K.*; Zhu, X.; Jia, F.; Auyeung, E.; Mirkin, C. A. “Temperature-activated nucleic acid nanostructures”, Journal of the American Chemical Society, 2013, 135, 14102-14015

Graduate and postdoctoral work: 40. Zhu, J.; Zhang, S.; Zhang, K.; Wang, X.; Mays, J. W.; Wooley, K. L.; Pochan, D. J. “Disk- cylinder and disk-sphere nanoparticles from block copolymer blend solution construction”, Nature Communications, 2013, doi:10.1038/ncomms3297 41. Wang, Z.; Zhang, K.; Shen, Y.; Smith, J.; Bloch, S.; Achilefu, S.; Wooley, K. L.; Taylor, J.-S. A. “Imaging mRNA expression levels in living cells with PNA∙DNA binary FRET probes delivered by cationic shell-crosslinked nanoparticles”, Organic and Biomolecular Chemistry, 2013, doi:10.1039/C3OB26923J 42. Ibricevic, A.; Gunsten, S. P.; Zhang, K.; Shrestha, R.; Liu, Y.; Sun, J.; Welch, M. J.; Taylor, J.-S. A.; Wooley, K. L.; Brody, S. L. “PEGylation of cationic, shell-crosslinked knedel-like nanoparticles modulates inflammation and enhances cellular uptake in the lung”, Nanomedicine: Nanotechnology, Biology and , 2013, doi:10.1016/j.nano.2013.02.006 43. Shen, Y.; Fang, H.; Zhang, K.; Shrestha, R.; Wooley, K. L.; Taylor, J.-S. A. “Efficient protection and transfection of small interfering RNA by cationic shell-crosslinked knedel- like nanoparticles”, Nucleic Acid Therapeutics, 2013, doi:10.1089/nat.2012.0390

4 44. Wang, Z.; Zhang, K.; Wooley, K. L.; Taylor, J.-S. A. “Imaging mRNA expression in live cells via peptide nucleic acid (PNA) strand-displacement activated probes”, Journal of Nucleic Acids, 2012, doi:10.1155/2012/962652 45. Zhang, K.; Hao, L.; Hurst, S. J.; Mirkin, C. A. “Antibody-linked spherical nucleic acids for cellular targeting”, Journal of the American Chemical Society, 2012, 134, 16488-16491 46. Auyeung, E.; Cutler, J. I.; Macfarlane, R. J.; Jones, M. R.; Zhang, K.; Mirkin, C. A. “Synthetically programmable nanoparticle superlattices: The hollow three-dimensional spacer approach”, Nature Nanotechnology, 2012, 7, 24-28 (cover article; highlighted by MRS Bulletin) 47. Zhang, K.; Zheng, D.; Hao, L.; Cutler, J. I.; Auyeung, E.; Mirkin, C. A. “ImmunoPods: Polymer shells with native antibody crosslinks”, Angewandte Chemie International Edition, 2012, 5, 1169-1172 (Hot Paper) 48. Pochan, D. J.; Zhu, J.; Zhang, K.; Wooley, K. L.; Miesch C.; Emrick, T. “Multicompartment and multigeometry nanoparticle assembly”, Soft Matter, 2011, 7, 2500- 2506 49. Cutler, J. I.; Zhang, K.; Zheng, D.; Auyeung, E.; Prigodich, A. E.; Mirkin, C. A. “Polyvalent nucleic acid nanostructures”, Journal of the American Chemical Society, 2011, 133, 9254-9257 (equal first author; highlighted by C&EN and WTTW Chicago Tonight TV program) 50. Zhang, K.; Cutler, J. I.; Zhang, J.; Zheng, D.; Auyeung, E.; Mirkin, C. A. “Nanopod formation through gold nanoparticle templated and catalyzed crosslinking of polymers bearing pendant propargyl ethers”, Journal of the American Chemical Society, 2010, 132, 15151-15153 51. Zhang, J.; Langille, M. R.; Personick, M. L.; Zhang, K.; Li, S.; Mirkin, C. A. “Concave cubic gold nanocrystals with high-index facets”, Journal of the American Chemical Society, 2010, 132, 14012-14014 52. Sun, G.; Berezin, M.; Fan, J.; Lee, H.; Ma, J. Zhang, K.; Wooley, K. L.; Achilefu, S. “Bright fluorescent nanoparticles for developing potential optical imaging contrast agents”, Nanoscale, 2010, 2, 548-558 53. Zhang, K.; Fang, H.; Wang, Z.; Li, Z.; Taylor, J.-S. A.; Wooley, K. L. “Structure-activity relationships of cationic shell-crosslinked knedel-like nanoparticles: Shell composition and transfection efficiency/cytotoxicity”, Biomaterials, 2010, 31, 1805-1813 54. Ma, J.; Bartels, J. W.; Li, Z.; Zhang, K.; Cheng, C.; Wooley, K. L. “Synthesis and solution- state assembly or bulk state thiol-ene crosslinking of pyrrolidinone- and alkene- functionalized amphiphilic block fluorocopolymers: from functional nanoparticles to anti- fouling coatings”, Australian Journal of Chemistry, 2010, 63, 1159-1163 55. Li, Z.; Ma, J.; Cheng, C.; Zhang, K.; Wooley, K. L. “Synthesis of hetero-grafted amphiphilic diblock molecular brushes and their self-assembly in aqueous medium”, Macromolecules, 2010, 43, 1182-1184 56. Li, Y.; Hindi, K.; Watts, K. M.; Taylor, J. B.; Zhang, K.; Li, Z.; Hunstad, D. A. Cannon, C. L.; Youngs, W. J.; Wooley, K. L. “Shell crosslinked nanoparticles carrying silver antimicrobials as therapeutics”, Chemical Communications, 2010, 46, 121-123

5 57. Zhang, K.; Fang, H.; Li, Z.; Ma, J.; Hohlbauch, S. V.; Taylor, J.-S. A.; Wooley, K. L. “Composite soft-matter nanoscale objects: nanocylinder-templated assembly of nanospheres”, Soft Matter, 2009, 5, 3585-3589 (cover article) 58. Zhang, K.; Fang, H.; Shen, G.; Taylor, J.-S. A.; Wooley, K. L. “Well-defined cationic shell crosslinked nanoparticles (cSCKs) for efficient delivery of DNA or PNA”, Proceedings of the American Thoracic Society, 2009, 6, 450-457 (cover article) 59. Fang, H.; Zhang, K.; Gang, S.; Wooley, K. L.; Taylor, J.-S. A. “Cationic shell-crosslinked knedel-like (cSCK) nanoparticles for highly efficient PNA delivery”, Molecular Pharmaceutics, 2009, 6, 615-626 (equal first author) 60. Li, Z.; Zhang, K.; Ma, J.; Cheng, C.; Wooley, K. L. “Facile synthesis of cylindrical molecular brushes by a sequential RAFT and ROM ‘grafting-through’ methodology”, Journal of Polymer Science, Part A: Polymer Chemistry, 2009, 47, 5557-5563 61. Zhang, K.; Fang, H.; Wang, Z.; Taylor, J.-S. A.; Wooley, K. L. “Cationic shell-crosslinked knedel-like nanoparticles (cSCKs) for highly efficient gene and oligonucleotide transfection of mammalian cells”, Biomaterials, 2009, 30, 968-977 62. Sun, G.; Fang, H.; Cheng, C.; Lu, P.; Zhang, K.; Walker, A. V.; Taylor, J.-S. A.; Wooley, K. L. “Benzaldehyde-functionalized polymer vesicles”, ACS Nano, 2009, 3, 673-681 63. Du, W.; Xu, Z.; Nystrom, A. M.; Zhang, K.; Leonard, J. R.; Wooley, K. L. “19F- and fluorescently labeled micelles as nanoscopic assemblies for chemotherapeutic delivery”, Bioconjugate Chemistry, 2008, 19, 2492-2498 64. Zhang, K.; Rossin, R.; Hagooly, A.; Chen, Z.; Welch, M. J.; Wooley, K. L. “Folate- mediated cell uptake of shell-crosslinked spheres and cylinders”, Journal of Polymer Science, Part A: Polymer Chemistry, 2008, 46, 7578-7583 (cover article) 65. Zhang, K.; Fang, H.; Chen, Z.; Taylor, J.-S. A.; Wooley, K. L. “Shape effects of nanoparticles conjugated with cell-penetrating peptides (HIV Tat PTD) on CHO cell uptake”, Bioconjugate Chemistry, 2008, 19, 1880-1887 Patents 1. Zhang, K.; Wang, D. “Poly(ethylene glycol) brushes for efficient RNA delivery”, Non- provisional US Application, Serial No. pending (based upon Provisional Application No. 62/646,542.) Assignee: Northeastern University 2. Zhang, K.; Tan, X. “Grafting-through polymerization of fully protected, hydrophobic oligonucleotides”, Non-provisional US Application, Serial No. pending (based upon Provisional Application No. Provisional Application 62/645,379.) Assignee: Northeastern University 3. Zhang, K.; Lu, X. “Brush polymer-assisted compaction of oligonucleotides”, International Application No. PCT/US16/45576 (Granted, Dec, 2019) Assignee: Northeastern University 4. Zhang, K.; Wang, D. “Amphiphilic polymer micelles and uses thereof”, Non-provisional US Application No.: 16/011,863 Assignee: Northeastern University

6 5. Zhang, K. “DNA nanostructures with improved biopharmaceutical properties and methods of making the same”, Provisional US Application No. 62/832,317 Assignee: Northeastern University 6. Mirkin, C. A.; Giljohann, D. A.; Daniel, W. L.; Cutler, J. I.; Zhang, K.; Zheng, D. “Templated nanoconjugates” EP Patent 2,494,075, 2012, US Patent 9,376,690, CN Patent ZL201080058178.1, JP Patent 5866119 Assignee: Northwestern University Published Presentation 1. Zhang, K. “Nanotechnology for intracellular nucleic acid delivery”, Henry Stewart Talks, Series: Nanomedicine, 2015, https://hstalks.com/bs/3028/

INVITED TALKS 1. “Polymer-assisted compaction of DNA – a new hope for antisense technology for oncology”, -US Society of Nanomedicine and Bionanotechnology Annual Meeting, July 2019, Hangzhou, China (invited) 2. “Targeting oncogenic KRAS in NSCLC using a novel polymer-antisense conjugate” ChinaNANO Conference, July 2019, , China (invited) 3. “Making oligonucleotides great again”, Department of Chemistry, University, July 2019, , China (Invited) 4. “Making oligonucleotides great again”, Department of Chemistry, National Normal University, April 2019, (invited) 5. “pacDNA for the treatment of mutant KRAS-driven lung cancer”, International Nanomedicine Conference, Sydney, Australia, June 2019 (keynote speaker) 6. “Systemic Antisense Oligonucleotide Delivery via Brush Polymers”, China-Europe Biomaterials Symposium, Guangzhou, China, May 2019 (keynote speaker) 7. “Making oligonucleotides great again”, Department of Chemistry, UMass Amherst, November 2018 8. “Making Oligonucleotides Better Biopharmaceuticals with Brush Polymers”, ChinaNANO, China, November 2018 (keynote lecturer) 9. “Making Oligonucleotides Better Biopharmaceuticals with Brush Polymers”, ChinaNanomedicine, China, November 2018 (keynote lecturer) 10. “Brush-architectured PEG as an effective gene regulation vector” ACS national meeting, August 2018 (PMSE Young Investigators' Symposium) 11. “Poly(oligonucleotide) - Bringing phosphodiester oligonucleotides to polymer chemistry”, ACS national meeting, August 2018 (invited TOSOH lectures) 12. “Making oligonucleotides great again”, IBS Symposium , Yonsei University, Korea, May 2018 (invited lecturer) 13. “Making oligonucleotides great again”, NanoKorea, Korea, May 2018 (invited lecturer) 14. “Making oligonucleotides great again”, World Polymer Congress, Australia, May 2018 (keynote lecturer) 15. “Making oligonucleotides great again”, Department of Chemistry, Boston College, May 2018 16. “Brush-architectured poly(ethylene glycol) for oligonucleotide delivery”, ACS national meeting, March 2018

7 17. “Self-assembled spherical nucleic acids from oligonucleotide-polymer conjugates for drug delivery and immunotherapy”, ACS national meeting, March 2018 18. “Making oligonucleotides great again”, Department of Chemistry, Rutgers University, January 2018 19. “Making oligonucleotides great again”, Department of Chemistry, South China University of Science and Technology, China, January 2018 20. “Making Oligonucleotides better drugs with brush polymer-assisted compaction”, Pacific Polymer Conference, Xiamen, China, December 2017 21. “Making oligonucleotides great again”, Department of Chemistry, Riverside, November 2017 22. “Making oligonucleotides great again”, Department of Materials Science, University of California Santa Barbara, November 2017 23. “Making oligonucleotides great again”, Department of Chemistry, University of California San Diego, November 2017 24. “Making oligonucleotides great again”, Department of Chemistry, University of California Los Angeles, November 2017 25. “Making oligonucleotides great again”, Department of Chemistry, , October 2017 26. “Making oligonucleotides great again”, Department of Chemistry, Texas A&M University, September 2017 27. “Making oligonucleotides great again”, The 6th International Conference on DNA Nanotechnology, Beijing, August 2017 28. “Spherical nucleic acids for stimuli-responsive delivery of dual payloads”, ChinaNano, Beijing, August 2017 29. “Antisense gene regulation with non-cationic polymer carriers”, ACS national meeting, April 2017 30. “Programmable brush polymer assembly via DNA hybridization”, ACS national meeting, April 2017 31. “Teaching oligonucleotides new tricks: A macromolecular approach”, Frontiers in Nanotechnology Seminar Series, Northwestern University, March 2017 32. “Teaching oligonucleotides new tricks: A macromolecular approach”, Program in Polymers and Soft Matter Seminar Series, MIT, March 2017 33. “Antisense gene regulation with non-cationic polymer carriers”, ACS national meeting, March 2017 34. “Programmable brush polymer self-assembly via DNA hybridization”, ACS national meeting, March 2017 35. “Biocompatible brush polymers for antisense gene regulation”, University of Massachusetts Medical School, March 2017 36. “Teaching oligonucleotides new tricks: A macromolecular approach”, Department of Chemistry, University of Connecticut, February 2017 37. “Overcoming the intrinsic biopharmaceutical difficulties of oligonucleotides and chemotherapeutics with their combination”, MRS national meeting, November 2016 38. “Teaching oligonucleotides new tricks: A macromolecular approach”, Department of Chemical Engineering, SUNY Buffalo, Buffalo, NY, October, 2016

8 39. “Teaching oligonucleotides new tricks: A macromolecular approach”, Department of Chemistry, Tufts University, Boston, MA, September 2016 40. “Teaching oligonucleotides new tricks: A macromolecular approach”, Department of Chemistry, University of Florida, Gainesville, FL, September 2016 41. “Utilizing the brush architecture for nanomedicine and materials self-assembly”, ACS national meeting, August 2016 42. “Making oligonucleotides better drugs with brush polymers”, International Forum on Biomedical Materials, Hangzhou, China, August 2016 (keynote) 43. “Teaching oligonucleotides new tricks: A macromolecular approach”, Institute of Functional Nano and Soft Materials, Soochow University, China, July 2016 44. “Teaching oligonucleotides new tricks: A macromolecular approach”, Department of Electrical Engineering, Chinese , Hong Kong, China, June 2016 45. “Non-cationic delivery of oligonucleotides using brush polymers”, Young Giants of Nanoscience, Hong Kong, China, June 2016 46. “Making oligonucleotides better drugs by brush polymers”, 7th International Symposium of Bioanalysis, Biomedical Engineering and Nanomedicine, Changsha, China, May 2016 47. “Teaching oligonucleotides new tricks: A macromolecular approach”, Department of Chemistry, University of Science and Technology in China, , China, May 2016 48. “Teaching oligonucleotides new tricks: A macromolecular approach”, Department of Chemistry, University, Hefei, China, May 2016 49. “Utilizing the steric congestion of brush polymers: From nucleic acid delivery to self- assembly”, ACS national meeting, March 2016 50. “Teaching oligonucleotides new tricks – A macromolecular approach”, Department of Chemistry, Mississippi State University, Starkville, MS, February 2016 51. “Sterically protected nucleic acids for supramolecular assembly and in vivo delivery”, Pacifichem, December, 2015 52. “Teaching oligonucleotides new tricks – A macromolecular approach”, Department of Chemistry, Jiaotong University, Shanghai, China, November 2015 53. “Brush polymers as non-toxic, biocompatible gene transfection carriers”, Department of Materials Science, Nanyang Technological University, Singapore, September 2015 54. “Self-assembly of nucleic acid amphiphiles”, ACS national meeting, August 2015 55. “Rational design of macromolecular superstructures”, ACS national meeting, August 2015 56. “Nucleic acid delivery – A tale of two Approaches”, Department of Materials Science, University, Zhenjiang, China, May 2015 57. “A carrier-free, light-activated nanoparticle system for the simultaneous delivery of nucleic acids and cancer drug”, ACS national meeting, March 2015 58. “Nucleic acid-mediated self-assembled nanostructures”, Department of Chemistry, Bridgewater State University, Bridgewater, MA, February 2015 59. “Gene delivery through structure design”, International Symposium on Bioanalysis, Biomedical Engineering and Nanotechnology, Changsha, China, May 2014

9 60. “Nucleic acid delivery – A tale of two approaches”, Department of Chemistry, University, Changsha, China, May 2014 61. “Nucleic acid delivery through structure design”, ACS national meeting, March 2014 (ACS Polymer Chemistry Award symposium) 62. “Supramolecular programming with nucleic acid-polymer conjugates”, Department of Applied Chemistry, of Technology, Nanjing, China, April 2014 63. “Supramolecular programming with nucleic acid-polymer conjugates”, Department of Chemistry, Nanjing University, Nanjing, China, April 2014 64. “Nucleic acid delivery through structure design”, Department of Materials Science, Shenzhen University, Shenzhen, China, April 2014 65. “Designing the connectivity between nanoscopic objects with DNA”, International Symposium on Bio, Organic, and Nanoelectronics, Nanjing, China, February 2014 66. “Nanopods – Hollow polymer and nucleic acid nanostructures”, World Polymer Congress, Blacksburg, VA, June 2012 67. “Teaching Oligonucleotides New Tricks – A Macromolecular Approach”, National Nanomedicine Seminar, February 2017 68. “Nucleic acid delivery through structure design”, Department of Pharmaceutical Science colloquium series, April 2014 69. “Nucleic acids as therapeutics: A nanotechnology approach”, IGERT nanomedicine course/seminar, November 2012 70. “What is nanotechnology?”, American Chemical Society Student Affiliates, Northeastern University seminar series, October 2012

RESEARCH SUPPORT External Support NIH NIGMS R01 (Active) “Making Oligonucleotides Better Biopharmaceuticals by Steric Protection” 11/2017-10/2022 1,560,693$ (Zhang sole PI) NSF CAREER Award (Active) “CAREER: Supramolecular Polycondensation of Polymer Brushes via DNA Hybridization” 03/2015-02/2020 525,000$ (Zhang sole PI) ACS PRF Doctoral New Investigator Award (Completed) “Controlling Polymerization Reactions at the Nanoscale” 10/2015-10/2018 110,000$ (Zhang sole PI) Rogers Corporation Collaborative Research Grant (Completed) “Novel thermosets for next-gen high-frequency applications” 05/2015-04/2016 140,500$ (Zhang sole PI) DOD Defense University Research Instrumentation Program (Completed) “Environmentally controlled infrared spectroscopy system for fundamental studies of polymer electrolyte membranes"

10 04/2014-03/2015 50,388$ (PI: Smotkin, NEU, Co-PI: Zhang)

Internal Support Northeastern University Tier 1 Research Development Award (Current) “Plasmonic Metamaterials: A Sustainable, Bottom-up Approach” 08/2018-07/2019 50,000$ (PI: Agar: 25,000$, co-PI: Zhang, NEU) Dana Farber Cancer Research Grant (Current) “Self-Delivering Adjuvant Nanoparticles for Cancer Immunotherapy” 08/2018-07/2019 50,000$ (PI: Zhang: 25,000$, Co-PI: Zhao, Harvard) Dana Farber Cancer Research Grant (Completed) “Making Oligonucleotides Better Cancer Biopharmaceuticals by Steric Protection” 08/2017-07/2018 50,000$ (PI: Zhang: 25,000$, Co-PI: Zhao, Harvard) Dana Farber Cancer Research Grant (Completed) “Making Oligonucleotides Better Cancer Biopharmaceuticals by Steric Protection” 08/2016-07/2017 50,000$ (PI: Zhang: 25,000$, Co-PI: Zhao, Harvard) Dana Farber Cancer Research Grant (Completed) “Development of a Novel Carrier-free Drug-DNA Nanoparticle System for Cancer Therapy” 08/2015-07/2016 50,000$ (PI: Zhang: 25,000$, Co-PI: Zhao, Harvard) Dana Farber Cancer Research Grant (Completed) “Development of a Novel Carrier-free Drug-DNA Nanoparticle System for Cancer Therapy” 08/2014-07/2015 50,000$ (Zhang: 25,000$, Co-PI: Zhao, Harvard) Northeastern University Tier 1 Research Development Award (Completed) “Plasmonic Metamaterials: A Sustainable, Bottom-up Approach” 08/2013-07/2014 50,000$ (PI: Zhang: 33,000$, co-PI: Liu, NEU) Northeastern University Start-up (Completed) 08/2012-07/2015 761,200$

TEACHING AND ADVISING New Course Developed Polymer Chemistry (Chem 5610) Course Description: Discusses the synthesis and analysis of polymer materials. Covers mechanisms and kinetics of condensation/chain-growth polymerization reactions and strategies leading to well-defined polymer architectures and compositions, including living polymerizations (free radical, cationic, anionic), catalytic approaches, and post- polymerization functionalization. Discusses correlation of chemical composition and structure to physical properties and applications. Courses Taught Fall 2019 Chem 2311 Organic Chemistry I Rating: 2.9 Enrollment: 150 Chem 5610 Polymer Chemistry Rating: 4.8 Enrollment: 6

11 Fall 2018 Chem 5610 Polymer Chemistry Rating 5.0 Enrollment: 6 Fall 2017 Chem 2311 Organic Chemistry I Rating: 3.5 Enrollment: 25 Chem 5610 Polymer Chemistry Rating: 4.8 Enrollment: 11 Chem 7730 Advanced Lab Techniques (seminars) Rating: 4.6 Enrollment: 8 Fall 2016 Chem 2311 Organic Chemistry I Rating: 4.3 Enrollment: 26 Chem 5610 Polymer Chemistry Rating: 4.9 Enrollment: 8 Spring 2016 Chem 2311 Organic Chemistry I Rating: 4.1 Enrollment: 39 Fall 2015 Chem 5610 Polymer Chemistry Rating: 4.3 Enrollment: 3 Summer 2015 Chem 7730 Advanced Lab Techniques (seminars) Rating 3.5 Enrollment: 22 S Spring 2015 Chem 5610 Polymer Chemistry Rating: 4.5 Enrollment: 11 Summer 2014 Chem 7730 Advanced Lab Techniques (seminars) Rating: 4.4 Enrollment: 21 Spring 2014 Chem 5610 Polymer Chemistry Rating: 4.3 Enrollment: 10 Summer 2013 Chem 7730 Advanced Lab Techniques (seminars) Rating: 3.8 Enrollment: 25

Postdoctoral Fellow Supervised Dr. Dali Wang (2016-2019) Shanghai Jiaotong University (China) Current position: postdoctoral fellow at MIT with Prof. Daniel Kohane/Robert Langer Dr. Zhang (2015-2016) Northeastern University Current position: Tencent Technologies (China) Dr. Hui Wang (2013-2013) University of Waterloo (Waterloo, Canada) Current position: Xerox Research Center (Canada)

Graduate Student Supervised Peiru Chen (Jan, 2019-present, PhD in Chemistry) Mengqi Ren (Jan, 2019-present, PhD in Chemistry) Jiansong Cai (Jan, 2019-present, PhD in Chemistry) Yang Fang (Jan, 2019-present, PhD in Chemistry) Yuyan (Audrey) Wang (Jan, 2018-present, Ph.D. in Chemistry)

12 Hao Lu (Mar, 2017-present, Ph.D. in Chemistry) Xueguang (Alex) Lu (Mar, 2013-July, 2017, Ph.D. in Chemistry) Thesis: “Nucleic acid-brush polymer conjugates for cellular gene regulation and programmable materials assembly”. Recipient of 2016 Dean’s Award for Graduate Student Excellence in Research and Provost’s Dissertation Award. Current position: postdoctoral fellow at MIT with Prof. Robert Langer. Fei Jia (Mar, 2013-Sep, 2017, Ph.D. in Chemistry) Thesis: “DNA-polymer conjugates: synthesis, physiochemical properties, and nanomedicine applications”. Current position: postdoctoral fellow at MIT with Prof. Robert MacFarlane. Xuyu (Travis) Tan (Mar, 2014-May, 2018, Ph.D. in Chemistry) Thesis: “Carrier-free nanoparticles systems for drug-DNA codelivery”. Current position: postdoctoral fellow at MIT with Prof. Bradley Pentelute Junjie Cao (Mar, 2015-May, 2016, non-thesis M.S. in Chemistry) Current position: Senior Chemist at Jordi Labs, LLC Yehui (Vi) Sun (Mar, 2018-May, 2019, M.S. in Chemistry) Thesis: “Poly(oligonucleotides)” Current position: Technical assistant at MIT Koch Institute; to join University of Washington as a PhD student Fall, 2019.

Visiting Scholars Hosted Dr. Lei Zhang Nanjing U. of Sci and Tech (Nanjing, China) 2020-present Xi Kang (Hefei, China) 2018-2020 Dr. Lanxia Liu Naikai University (, China) 2017-2018 Dr. Xueyan Cao (Shanghai, China) 2016-2017 Dr. Hui Li (Changsha, China) 2015-2016 Bohan Zhang Hunan University (Changsha, China) 2015-2016 Dr. Aysegul Oncul Bahcesehir University (Istanbul, Turkey) 2013, 2015

SERVICE AND PROFESSIONAL DEVELOPMENT Service to the Institution • Student Awards Committee Member (2012-present) • Graduate Recruitment Committee Member (2012-present) • Faculty Advisor for Majors (2016-present) • Faculty Marshal (2014) • Faculty Advisor for the student group Northeastern Taichi Association (2014-present) • Presenter in Northeastern University Student Affiliate of the ACS seminars (2012) • Presenter in Chemistry REU program seminars (2014-present) • Presenter in IGERT Nanomedicine program seminars (2012, 2019) • Reviewer for Provost Office’s Thesis/Dissertation Grant (2013, 2015-present), Outstanding Graduate Student Research Award (Life Sciences/Physical Sciences/Engineering) (2016-present)

13 • Member of the College Council (2019-present)

Service to the Discipline • Editorial Board member for the journal iScience (Cell Press, 2019-present) • Ad hoc panel reviewer for National Science Foundation (2013-2018) • Mail reviewer for National Science Foundation (2014-present) • Symposium organizer at Pacifichem 2020 (2020) • Ad hoc panel reviewer for National Institute of Health, BMBI study section (2013) • Referee for: Journal of the American Chemical Society, Biomacromolecules, Journal of Polymer Science: Part A Polymer Chemistry, Nanomedicine, Molecular Pharmaceutics, Journal of Controlled Release, ACS Nano, Nanoscale, Nanomedicine NBM, ACS Macro Letters, Advanced Materials, Chemical Reviews, Bioconjugate Chemistry, Scientific Reports, Advanced Functional Materials, Small, Nature Communications, Macromolecules, European Polymer Journal, Journal of American Water Resources Association, Macromolecular Rapid Communications, Current Pharmaceutical Design • Overseas reviewer, Nanyang Technological University Tier 1 Grants (2016) • Co-organizer of the international conference Young Giants of Nanoscience, Hong Kong (Summer 2016) • Chaired conference sessions: ACS national meetings (Fall 2015, Spring 2016), Young Giants of Nanoscience Conference, Hong Kong (Summer 2016), International Forum on Biomedical Materials, Hangzhou, China (Summer 2016), International Conference on DNA Nanotechnology, Beijing, China (Summer 2017), ChinaNano, Beijing, China (Summer 2017) • Professional Society Membership: ACS Division of Polymer Chemistry (2007-present), ACS Division of Polymeric Materials: Science and Engineering (2007-present), Materials Research Society (2015-present)

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