Boldly Going Where No Group Has Gone Before: Medical Genomics in the Wilds of Deepest Darkest North Queensland Alan G Baxter James Cook University, Townsville, Qld

NEWSLETTER PP 100000910 ISSN 1442-8725 June 2014

Boldly Going Where No Group Has Gone Before: Medical Genomics in the Wilds of Deepest Darkest North Queensland Alan G Baxter James Cook University, Townsville, Qld.

In 2003, my research group relocated from the Centenary would continue in my new the Centenary Institute, on the Royal Prince home. I had attributed these successes to my Alfred Hospital campus and adjacent to the own abilities to judge people, and, knowing University of Sydney, to the Townsville that JCU had a world class marine biology Campus of James Cook University (JCU). research endeavour, assumed that we would At that time, the University had not held have little trouble in recruiting skilled and NHMRC funding for over a decade. The enthusiastic staff. In retrospect, this was a last (and only previous) recipient had moved mistake. JCU had a strong “Tech College” there on a training fellowship and moved mentality (it was formed by a merger between away again within a year. The campus a teaching college and a school of the consisted of brutalist concrete bunkers University of Queensland) and undeniably adorned with concrete sheets protecting the tropics attracts, as Noel Coward used to windows from cyclone damage, and wide note, some odd sorts. I failed to follow the expanses of the area’s original fl ora – a dry, wisest advice I have ever received, that of Jon sparse woody scrub and native grassland Sedgwick when he said, “Only ever employ populated by wandering mobs of wallabies, nice people.” I learned my lesson, and after soaring cacophanies of cockatoos, and a many attempts to change the lab culture, vibrant collection of tropical butterfl ies. our original expeditionary force rebooted and repopulated the lab – this time carefully Our lab was carved out of the ground fl oor observing the Sedgwick rule. of the largely derelict, but still occupied, Alan Baxter Molecular Sciences Building. Various JCU has some of the best administrative staff physical restrictions crimped the amount of I have met anywhere: bright, enthusiastic offi ce space, but the lab design was practical and constructive. Of course, like anywhere, and safe, and far better than the rows of it also has some who are not so helpful, but Contents glass boxes architects seem to prefer. A unlike some larger institutions, none of these new animal facility was built specifi cally to are malevolent. In addition, we received Boldly Going ... 1 house the kind of work we do, which is the very strong support from the Faculty. I had Editorial 3 study of gene/environment interactions in been recruited to establish a viable medical President’s Column 11 autoimmunity. This required very fi ne levels research endeavour and to a considerable Honorary Secretary’s News 12 of environmental control and the resulting extent, I was given a free rein. The Research facility – the fi rst building on the campus to Offi ce was refocused and prioritised to act Upcoming Conferences 12 have an exterior colour scheme – remains as facilitators, rather than gate-keepers, of Visiting Speaker Program 13 one of the best small animal facilities in funding. Finance reporting was changed to Three to One! 16 Australia. provide year-by-year accounts, instead of whole-of-grant double entry book-keeping. DoI 2014, Victoria 20 In moving, most of our, and certainly all of A gradual acceptance grew that competitive Councillors’ News 25 my, attention was focussed on practical and research was hard and that teaching loads Travel Award Conference Reports 29 physical requirements. To a large extent, I had to be seriously lightened for productive had assumed that the decade-long run of researchers. Publications List 33 gifted staff and students I had enjoyed at cont.p4 ASI Inc. Newsletter June 2014

ASI Inc. COUNCIL Non-Voting Councillors: Newsletter Editor President Vice President Dr Simon Apte Professor Dale Godfrey Professor Christopher Goodnow Ph: 61 7 3362 0380 Dept Microbiology & Immunology Department of Immunology Email: [email protected] Peter Doherty Institute JCSMR, ANU University of Melbourne Parkville Vic 3010 PO Box 334, Canberra ACT 2601 Journal Editor Ph: 61 3 8344 6831 Ph: 61 2 6125 2394 Dr Gabrielle Belz Email: [email protected] Email: [email protected] Ph: 61 3 9345 2544 Fax: 61 3 9347 0852 Email: [email protected] Honorary Secretary Honorary Treasurer A/Prof. Stuart Berzins Dr John Stambas Visiting Speakers Co-ordinator CRN Section, School of Health Sciences AAHL, CSIRO Deakin Collaborative Lab. A/Prof. J. Alejandro Lopez Federation University Australia Private Bag 24 Ph: 61 7 3845 3794 Fax: 61 7 3845 3510 Mt Helen Vic 3352 East Geelong Vic 3220 Email: a.lopez@grifﬁ th.edu.au Ph: 61 3 5320 2039 Ph: 61 3 5227 5740 Email: [email protected] Email: [email protected] Day of Immunology Co-ordinator Dr Claerwen Jones State Councillors Ph: 61 3 8344 9595 Fax: 61 3 9347 1540 New South Wales Victoria & Tasmania Email: [email protected] Dr Marcel Batten Dr Daniel Gray Ph: 61 2 9295 8412 Ph: 61 3 9345 2497 Meeting Co-ordinator Email: [email protected] Email: [email protected] Dr Susanne Heinzel Ph: 61 3 9345 2609 Fax: 61 3 9347 0852 Queensland South Australia & Northern Territory Email: [email protected] Dr Kristen Radford Dr Cara Fraser Ph: 61 7 3443 7638 Ph: 0422 903 093 Council Member of IUIS Email: [email protected] Email: [email protected] Professor Alan Baxter Ph: 61 7 4781 6265 Western Australia Australian Capital Territory Email: [email protected] Dr Andrew Currie Dr Anselm Enders Ph: 61 8 9360 7426 Ph: 61 2 6125 7605 FIMSA Councillor Email: [email protected] Email: [email protected] Professor Alan Baxter Ph: 61 7 4781 6265 New Zealand Email: [email protected] Dr Roslyn Kemp Ph: 64 3 479 7708 Honorary Archivist: Email: [email protected] Dr Judith Greer Project Manager Ph: 61 7 3346 6018 Miss Sarah Fardy Email: [email protected] Ph: 61 3 5227 5794 / 0413 917 990 ICI2016 Councillor Email: [email protected] Professor Jose Villadangos Administrative Correspondence Ph: 61 3 9035 7684 Ms Judi Anderson Email: [email protected] ASI Inc. Secretariat PO Box 7108 Upper Ferntree Gully Vic 3156 Ph: 61 3 9756 0128 Fax: 61 3 9753 6372 Email: [email protected]

The New ASI Website The ASI web site (www.immunology.org.au) has been fully remodelled and updated. New services include: Links providing members with free access to Immunology & Cell Biology, Nature Immunology, Nature Reviews Immunology Special offers for ASI members Download and upload forms for ASI awards Positions vacant page Online membership renewal Upcoming conference listings Women's initiative Twitter feed as well as many links to sites of immunological interest at home and abroad. If you would like to advertise a job or conference, or if you have an immunology news story, or a favourite immunology-related site that you would like to see linked to the ASI website, please email Sarah Fardy at [email protected]

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EDITORIAL Confusing Times for Medical and children under 16 will have to pay for the Thanks to Alan Baxter for his entertaining Researchers fi rst 10 visits per year – now I know some article, it’s a great perspective on a very people go to their GP more than once every interesting career. It really is fantastic It was only three years ago we were fi ghting 5.2 weeks, but many of those people will be that contributors like Alan and his team against proposed cuts to the NHMRC concessional patients and won’t contribute fi nd the time to put an article together for budget that would have more-than halved more than $50 p/a to the fund. Now my maths the Newsletter. I like his ideas about only the available funding. Now, without even a ain’t so good, but, best-case scenario: if every employing nice people, unfortunately that’d fi ght, it seems we are in for the sort of support woman, man and child goes to the GP 10 probably rule me out of a job in his lab – I we wouldn’t have dared dream of with the times/year – that gives us $50 x 22,680,000 guess I wouldn’t make it past the ice bucket Government’s proposed Medical Research = $1.3bn p/a into the fund, x 8 years (+ a anyway! Future Fund. This proposed fund is predicted kickstart of $1bn of uncommitted funds from to reach $20bn by 2023 and will act as an the existing Health and Hospitals Fund) gives Thanks also to other contributors in this endowment – where revenue from the fund us a fund of $10.7bn. Revenue from the fund edition, including Claerwen Jones and will act as a revenue stream to the NHMRC. will apparently add to the NHMRC revenue Susan Christo. Their reports on the Day That’s great news for medical research but stream from 2015-16 (so not compounding); of Immunology in Victoria and the Tri- does come at a loss to other areas of scientifi c that means that more than $9bn will have to University Dinner in Adelaide are great research. As my kids would say – too bad, so be made up by other health reforms. This examples of ASI reaching out and sharing sad; but hang on, before you exercise your leaves me in a confused state and more than the marvel of immunology. Schadenfreude, something doesn’t seem to a little concerned for those areas of health add up. The fund will be paid for by the $7 that will be losing $9bn– anyway let’s see Simon Apte co-contribution for visiting the GP, with $5 of what gets through the Senate – I’m sure it that going to the fund. Concessional patients will all be clear then.

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Boldly Going Where No Group Has Gone Before ... (cont.)

One of the biggest challenges we faced was trying to embed a culture of excellence within an institution that has a dominant role in remedial education. Everyone paces themselves against the people surrounding them, and students struggle to see the point in hard work if the same qualifi cation is being achieved by others without it. Central to our strategy for addressing this was to establish a research centre, the Comparative Genomics Centre (CGC), with which we hoped to create a microculture to nurture the scientifi c endeavour. The evolutionary biologists Ross Crozier and David Miller played a major role in the early days of the Centre as both had already established molecular labs at JCU prior to our arrival. Given the relative scarcity of molecular biology laboratories at JCU, the Centre was formed by an aggregation of labs from a wide range of sub-disciplines. Our seminar series was, Medical Genomics Group LtoR Back Row: Adrian Gemiarto, Ben Crowley, Alan Baxter, Laura Kolter and remains, an extraordinary mix of talks Middle Row: Xuyen Dinh Thi, Tiange Liu, Tammy Dougan on biochemistry, coral biology, crop plants, Front Row: Margaret Jordan, Zizi Molaee, Letitia Smith molecular technologies, human disease, fl y models of chromosome disorders, mouse expanded the University’s operations and mouth has recruited a team of undergraduate models, in vitro work … in fact, almost any footprint. The new buildings bring colour students who prefer laboratory work over bar molecular analysis of health and disease in and activity to the campus. Some older work. Some we lose to the medical course; almost any species. buildings have been renovated, cafes and others we hope will stay for Honours. This other services have been established within part-time, casual workforce supplements our The CGC currently has 92 members across them, pavements laid to connect them, and postgraduate research students and full-time ten research groups with an annual research the surrounding grounds more carefully staff, greatly contributing to a wonderful budget exceeding $9M. It has become an tended. laboratory environment characterised by administrative and support structure that cheerful productivity. prioritises research and is able to balance, to The latitude in approach allowed us by the an extent, the sometimes competing needs University turned out to be necessary. The Our laboratory studies the immunogenetics of undergraduate teaching as represented by nature of human resources available for of autoimmunity, with a particular focus on the Schools. It provided for the University building a viable laboratory in the tropics gene/environment interactions and innate a template by which some of the research differs greatly from that available in major (or innate-like) immunity. We rely heavily needs of both research fellows and mixed population centres. I very quickly found on the ability to model the effects of genetic duty academics could be met with reasonable that relatively few local students were variation in mice. effi ciency and economy. In the last few interested in biomedical research and that years, JCU has taken this template and some postdocs and research assistants who For example, in collaboration with the applied it across the whole university, so applied were not predominantly interested laboratory of A/Prof. Helmut Butzkueven that we now have twelve research Centres in the work. We had to develop a new style at the Royal Melbourne Hospital, we are covering everything from Language and of interview; one that now includes the examining gene expression of fi ve peripheral Culture Research to Sustainable Fisheries memorable line, “Having noted that you list blood leukocyte subsets from patients with and Aquaculture. PCR amongst your skills, we have prepared Multiple Sclerosis (MS) and healthy controls. an ice bucket of reagents in the lab for you This data set is being correlated with the Over the last ten years, the campus has …” In the end, the solutions to many of these subjects’ genotypes at previously identifi ed changed enormously. We are currently problems found us, rather than the other way susceptibility loci in order to identify working on the plans of a new teaching and round. Our web presence now attracts an expression quantitative trait loci associated research building for the (mostly) biological excellent calibre of internationally funded with the disease. This data set of over 730 sciences – the fi fth I have been involved students. We host a procession of backpackers microarrays also provides an opportunity to in planning since we arrived. The Medical and students on internships who leave reports identify differences in gene network usage School, and the affi liated adjunct health of their experiences on notice boards, both and active genetic pathways between patients training programs that were launched around virtual and real, and encourage others to and healthy controls, both systemically and the time of our relocation, have greatly follow in their footsteps. Similarly, word of within individual leukocyte subsets. These

4 ASI Inc. Newsletter June 2014

Dr Margaret Jordan, Betty Cuthbert NHMRC/MSRA Early Career Research Fellow

Histology Group:

LtoR: Karinne Law, Thea Buenviaje, Jenna Stewart

I obtained my PhD in 2011 from JCU, Townsville under the supervision of Prof. Alan Baxter. I was born in Scotland, but data provide key information about specifi c For example, some of these NKT cell control spent my formative years in South Africa. MS candidate genes: the associated protein’s genes affect lipid catabolism, and we are now It was here that I attained a BSc degree, coding region, its direction of differential dissecting how differences in the lipidome at the University of the Witwatersrand. I expression, and the cell type that expresses affect immunity. subsequently worked as a Medical Scientist in this difference. With this information, we Cytogenetics at the South African Institute for can produce transgenic or targeted gene A third example stems from our previous Medical Research for 11½ years, managing defi cient mice to model the human disease- work on the effects of bacterial constituents the laboratory and performing the culture, associated genotype in a mouse model of on type 1 diabetes (T1D), lupus and MS. analysis and karyotyping of human blood, MS. These experiments will produce not After having identifi ed by chance bacterial bone-marrow, amniotic fl uid and chorionic only formal validation of these genetic products that could exacerbate and inhibit villus samples. Over that period, I set up the candidates but also a platform for testing autoimmune disease (depending on the Fluorescence in situ Hybridisation (FISH) potential therapeutics. product and the disease), we attempted a node of the Cytogenetic Unit which permitted more systematic study of the role of Toll- further research into chromosome breakage In another project, we have taken the like receptors (TLR) in autoimmunity. This and translocations as well as interphase same approach of correlating genetic and project is now focussed on the effects of analyses of numerical anomalies. I cloned expression data, to study the genetic control TLR deletion on T1D. Some TLR-defi cient and prepared centromeric probes myself, of the innate-like lymphocyte population, mutants are protected; in some, disease is while also using commercially obtained NKT cells. We conducted a genetic linkage exacerbated. Remarkably, the strains that chromosome paints. While working as a study to identify two regions associated show worse disease mimic three intestinal Medical Scientist I studied Psychology and with differences in NKT cell numbers in phenotypes identifi ed in children at the onset subsequently completed an Honours degree mice. These data were then used to guide of T1D; changes in microbiome, changes in in Human Cytogenetics on Uniparental the production of congenic mouse lines for intestinal immunity and structural defects in Disomy in Silver Russell Dwarfi sm. each locus, and then expression microarray the mucosal barrier. These three phenotypes studies identifi ed, within each congenic were originally thought to be independent In 1998 I moved to Australia on a work visa interval, the relatively small numbers of risk factors which, if they co-occurred, and worked in Cytogenetics for nine months differentially expressed genes. Again, could generate the “perfect storm” leading before successfully applying for a position this information was suffi cient for us to to pancreatic beta cell destruction. Our new in the Autoimmunity Research Group at the construct mutant mice to formally test data show them to be, not independent but, Centenary Institute in Sydney. There I worked the role of each candidate. Studies of this parts of a causal chain. We are currently as a Research Assistant on genetic linkage nature are really quite unpredictable. While studying the nature and direction of these projects in mouse models of autoimmune we originally assumed we would primarily causal links. disease, to identify genes involved in SLE, fi nd “immune” genes, our experience has Type 1 diabetes and Gastritis as well as the been that we depend more and more on the Please let me introduce the members of the immunoregulatory cell subset, NKT cells. biochemical and cell biological expertise of team: On obtaining Australian residency and our collaborators to understand the molecular citizenship, I applied for and was granted mechanisms by which these genes operate.

5 ASI Inc. Newsletter June 2014 a scholarship to undertake a PhD in the developed. I carried out my PhD studies in that I established mouse islet isolation and Medical Genomics Group when the group the Vascular Biology Unit at JCU, where my transplantation techniques, which provided relocated to Townsville. My PhD project research interests focussed on understanding required tools to investigate how immune used mouse models of autoimmune diseases, the cellular mechanisms of infl ammation cells destroy insulin-producing beta cells congenic dissection of gene linkage regions, within abdominal aortic aneurysms, by which of pancreas. gene expression microarray analyses, qPCR innate immune responses are initiated and and sequencing as tools to identify genes regulated. I am now working as a Postdoctoral I joined the Medical Genomics Lab in 2013, controlling NKT cells, as well as genes Research Fellow in the laboratory of Prof. as I had skills in performing the specialised contributing to experimental autoimmune Alan Baxter at JCU Townsville focusing mouse islet procedures and analysis. I am gastritis. The function of two of these genes on NKT cell biology, and the associations currently working on TNF-related apoptosis- was subsequently confi rmed through my between NKT cells and autoimmune diseases inducing ligand (TRAIL/TNFSF10), which production of transgenic and knockout mice. like T1D. My current project focuses on is a typical member of the structurally My research on genes controlling NKT cell the metabolic control of NKT cells and related TNF superfamily and Fas-FasL numbers has progressed from the initial the role of lipid metabolism in innate-like mediated beta cell apoptosis in islet cells. localisation by genetic linkage analysis, lymphocytes. I am attempting to identify the role TRAIL to the identity of likely candidate genes plays in the inhibition of cytokine secretion by congenesis, to the confi rmation of the Dr Tharun Mysore, Postdoctoral Research and amelioration of insulitis in the NOD candidate by transgenic complementation. Associate mouse model of T1D. I will be dissecting the signalling pathways activated when TRAIL My most recent research dissects the on the surface of islet beta cells is ligated, molecular mechanisms of MS susceptibility. using Genomics and protein techniques. In This project provides a vehicle for formally the future, I’d like to identify circulating testing three candidate genes that are very pre-T1D biomarkers suitable for a high strongly associated with MS in humans, throughput-screening platform. and will fundamentally advance our understanding of the aetiology of MS Mr Benjamin Crowley, Executive and by testing not only these gene/disease Research Assistant associations, but also allele/cellular function associations in both mice and humans.

Ms Tammy Dougan, Research Fellow

Upon completion of my MAppSc (Microbiology & Biotechnology) at Royal Melbourne Institute of Technology (RMIT) in Melbourne, I researched for over two years in Molecular Genomics in Syngene International Pvt Ltd, which is a leading contract research biotechnology company in India. In this position, I was involved in developing high-throughput assays for screening combinatorial compounds I joined the Medical Genomics Group in for treating human diseases. During 2010, initially to provide administrative this time, I developed a keen interest in assistance to Prof. Alan Baxter, while at the diabetes as this slow debilitating disease same time studying my BSc in Biochemistry, I originally came from Kings College, affected several members of my family. Molecular Biology and Botany at JCU. London, UK, where I completed an MSc in On joining the Immunology Research Since 2012, I’ve had a greater role within Immunology while I worked as a Research Centre (IRC), University of Melbourne, the lab, working at the bench. Currently, Assistant at Imperial College. The projects I as an RA in 2001, I gained insights into together with the post docs and research contributed to were focused on the analysis Transplantation Immunology and applied students, I am working on several projects, of the afﬁ nity of autoantibodies in sera from these skills in my PhD there to develop including investigating the effects of TLR patients with Goodpastures’ disease and transgenic mouse models for studying human on the intestinal microbiome, and how they autoimmune Vasculitis. It was at Imperial diseases, including diabetic nephropathy inﬂ uence intestinal permeability. In other College that my interest in autoimmunity and cardiomyopathy. It was in the IRC work, I am helping Margaret Jordan and

6 ASI Inc. Newsletter June 2014

Tammy Dougan dissect the mechanisms I graduated with a BPharm in 2006 from by which innate-like T cells are affected by the Widya Mandala Catholic University in lipid metabolism. Indonesia. Then I completed a Masters degree in Biotechnology at UCSI University in Ms Letitia Smith, Research Assistant Kuala Lumpur, focused on the identifi cation of an anti-pathogenic compounds derived from propolis (a mixture of fl ower resins and bee enzymes used to line the beehive, making it sterile) from manuka fl owers. In 2013, I received a JCU Postgraduate Research Scholarship to pursue my PhD degree in the Medical Genomics group led by Prof. Alan Baxter.

My PhD is focused on MS susceptibility genes, which have been identified by combining genomic data from association studies and microarray analyses. I am investigating how changes in the expression Xuyen Dinh Thi of these genes affect the functions of immune cells, especially monocytes and NK cells, and Medical Technical University and taught into lead to the development of MS. To answer the BMedLabSci degree in the Department this question, I am using gene-silencing of Biomedical Science. and lentiviral transduction techniques to modulate the target genes’ expression in human primary cells and also in-vivo I completed my BSc (Hons) at the University In 2011, I was awarded an Australian Development Scholarship from the Australian using knock-out mice in Experimental of Melbourne in 2002, majoring in Genetics Autoimmune Encephalomyelitis (EAE). and Immunology. My honours year involved Government to study for a PhD. I joined the Medical Genomics Group and am interested mutation detection in one of the major breast Mark Wilkes, Off-Campus Postgraduate cancer-predisposing genes, BRCA1, in a in NKT cell development. I am using fl ow cytometry to investigate the factors affecting Research Student studying at the Mayo study of Australian women with early onset Clinic, Rochester, Minnesota, USA breast cancer and a strong family history. For the numbers and differentiation of NKT several years I worked at the University of cells using a mouse model transgenic for the Melbourne under the supervision of Prof. NKT cell T cell receptor. During my study Melissa Southey, investigating the genetic under the supervision of Prof. Alan Baxter, causes of breast, colorectal and prostate I have learned a lot about immunology and cancers within various population- and clinic- immunological techniques, which will be based studies. My work primarily involved very useful for my future career. detection of genetic variants such as SNPs and large alterations (deletions/insertions). Adrian Gemiarto, Postgraduate Research Student After a few years away from science during which I travelled and lived overseas, I returned to Australia and joined the Molecular Genomics Group at JCU. Currently I work with Dr Margaret Jordan on a project investigating the gene expression of immune cells in Multiple Sclerosis, using the Affymetrix microarray platform. I also contribute data to other gene expression projects within the laboratory. When I began my undergraduate studies I Xuyen Dinh Thi, Postgraduate Research was intent on pursuing marine biology at Student JCU. It had been my desire since being eight I come from Vietnam. In 2007, the cloning years old. However, fi rst through a classical of Dolly the Sheep directed my interest genetics class, and then a section on basic into biotechnology. I studied a BBiochem biochemistry in a zoology class I discovered at Vietnamese National University, Hanoi. a passion for biochemistry and molecular After graduating with a Masters, I worked biology. During my last 18 months as an as a lecturer and researcher at Hai Duong 7 ASI Inc. Newsletter June 2014 undergraduate I volunteered in the labs of Subhash Vasudevan and Jim Burnell with independent research projects. Before my fi nal year I took six months away from JCU to visit family, where I worked as a Special Research Associate in the laboratory of Edward Leof at the Mayo Clinic. The time spent here galvanized my interest in biochemistry and I returned to JCU highly motivated.

Upon completing my bachelor degree I returned to Edward Leof’s lab for what I intended to be a short stint before going on to graduate school. My research blossomed into a broad new direction and I found myself unable to leave it for graduate school. After 12 years, I was fi nally convinced to get my PhD and enrolled at JCU. Although my thesis project is in the fi eld of TGF-beta signaling Tiange Liu Karinne Law (as it was for the previous 12 years), we opted for a completely new direction for my thesis, Baxter’s laboratory at JCU. whose research coincides with the interest or a direction that has begun to yield fruit after major of the student) in their fi rst year. I was substantial efforts. My project is to build a better mouse model for fortunate enough to be assigned to Professor MS. To examine the putative role of Epstein- Alan Baxter at JCU’s CGC. Prof. Baxter As a student studying remotely, my direct Barr Virus (EBV) in MS, I’m generating presented me with a tremendous opportunity contact with my lab colleagues is limited. My the genetically modified mouse strains in my fi rst year, whereby he offered me a work focuses on the Transforming Growth where the effects that EBV has on human B casual position in the lab to learn important Factor β and the mechanisms of how this cells are mimicked by two transgenes. The practical skills, and immerse myself in the non cell-permeable cytokine transmits the transgenic mice will be monitored clinically research culture of the CGC. Now in my third intracellular signal from the cell surface into and studied by histopathology and fl ow year of full-time undergrad, I co-ordinate the nucleus where gross genetic modifi cations cytometry. In particular, I’m focusing on the histology components of a couple of ongoing occur. Interestingly, the cellular responses to pathogenic role of B cells in the initiation and projects in the lab, and am in the process of TGFβ vary considerably depending on the development of MS. I’m also working on an training a new student, Jenna Stewart. cell type, yet all cells signal through the same additional project, which is to examine the surface receptors and many of the signalling relationship between NKT cell numbers and Ms Jenna Stewart, Undergraduate Casual molecules are shared. While new signalling function and susceptibility to EAE. I induce Research Technician molecules continue to be identifi ed, our active EAE on Vα14 transgenic and CD1d understanding of how and where currently knockout mice that have different numbers identifi ed factors act, as well as how the of NKT cells. Recently, I have found varying cell regulates these processes, remains severity of paralysis in these mouse strains, incomplete. To that end, we have focused and a higher level of NKT cells appears on known TGFβ signalling proteins and to protect mice. I have gained experience pathways and examined how cells regulate in presenting my research nationally and entry of these messengers into the nucleus. most recently received a student poster A number of novel proteins and mechanisms prize in the Day of Immunology Event 2014 have been identifi ed, some of which appear to (Townsville). Medical genomics is a very play roles in deregulation of TGFβ signalling dynamic group with many fantastic projects during disease progression, while others and superior scientifi c staff to work with. further defi ne how the cell propagates the TGFβ signal to regulate the genome during Ms Karinne Law, Undergraduate Casual homeostasis of various cell types. Research Technician I was originally from a small regional town Tiange (Susan) Liu, Postgraduate in South East Queensland, where I graduated Research Student high school in 2011. I was then accepted In 2012, I received my bachelor degree in into the BSc (Advanced) program at JCU in animal science from Northwest Agriculture Townsville, and I commenced my studies in and Forestry University in Yangling, China, February 2012. The nature of the JCU BSc I am from Rockhampton and decided to move and was then awarded a JCU-CSC Joint Advanced program is unique in that students to Townsville to study science at JCU. I am Scholarship for my PhD studies in Prof. are assigned to a mentor (a senior academic currently in third year studying in the BSc

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Advanced program majoring in Genetics Ms Laura Kolter, Casual Research Ms Zeynab Molaee, Visiting Scientist and Genomics. I have recently joined the Technician/Visiting Scientist Baxter lab and am working with Karinne Law to provide histology services, including quantitative analyses, for the laboratory.

Ms Athea Buenviaje, Undergraduate Casual Research Technician

I come from Iran and received my BSc with a major in genetics there. I then moved to lovely I have only just arrived, so I am not quite sure New Zealand and updated myself in graduate what I will be working on! I studied Pharmacy level of molecular genetics in Massey at the Friedrich-Wilhelms-University University. Recently, I moved to Townsville in Bonn, Germany. During my studies and connected with the Immunonogenetics I completed a course in Pharmaceutical research program at JCU. I'm very proud of I was originally from Edmonton, Alberta, being in Alan Baxter's team and working Canada, where I graduated high school Biology and an introduction to Molecular and Cellular Biology. After the second with great people. I am currently genotypying at Archbishop O’Leary High school in several different mice strains of mice in the 2007. I then moved to Ontario in Guelph state examination I wanted to gain more experience in the lab and Alan Baxter has lab and I enjoy doing it. With falling price where I was accepted to the Biomedical of sequencing, this is a great time for me to Science program at the Ontario Veterinary given me the opportunity to work for six months at the CGC. learn about the genetics of complex diseases. College, University of Guelph. I graduated In near future, I would like to learn about with Honors in 2011. I recently moved to newly identiﬁ ed genetic associations which Australia to enrol as a medical student at JCU can provide deeper insights into the aetiology in Townsville. Throughout my biomedical of medical conditions. science career I have been involved in a series of volunteer and research assistantships and have had the opportunity to work in several research labs. I became interested in studying human diseases and conditions with an eye towards improving human health. I also enjoy the atmosphere of the laboratory and the co-operative spirit of researchers. My undergraduate research experiences ingrained in me the systematic approach that is required to effectively direct research and solve problems. In particular, I became ICB & CTI Online Manuscript Submission interested in the area of Molecular Biology Online manuscript submission for Immunology & Cell Biology and and Genetics and was recently fortunate to Clinical & Translational Immunology now available via: become part of the Medical Genomics Group http://mts-icb.nature.com/ under Prof. Alan Baxter at JCU’s CGC. http://mts-cti.nature.com/ Though research presents many intellectual challenges, I wish to be involved both in All manuscript submissions to ICB and CTI should in future be made online via these websites to speed up the reviewing and acceptance of manuscripts. traditional primary care and in investigative research that I hope will lead to more effective Gabrielle Belz, Editor-in-Chief treatments of human diseases. Immunology & Cell Biology Clinical & Translational Immunology

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10 ASI Inc. Newsletter June 2014

PRESIDENT’S COLUMN

Dear ASI members, this part of the website, and take advantage of this important opportunity to help progress Firstly, I am pleased to say that as I am the careers of females in our fi eld. Please approaching the end of my term as President also see our website for more details on of ASI, the Society appears to be happy and this program http://www.immunology. healthy. Membership numbers continue to org/womens-iniative/ and to register for the climb and are up from this time last year, our mentorship program or to be listed on the annual meeting last year was a great success database of female immunologists, please both scientifi cally and it also returned some contact Roslyn Kemp (roslyn.kemp@otago. funds to the Society – again, many thanks ac.nz). to Anne La Flamme and the LOC in New Zealand for a terrifi c job with this meeting. We have seen another highly successful We are tracking toward another great meeting Day of Immunology this year and I want to In response to the survey we conducted last this year in Wollongong (a joint meeting thank Claerwen Jones, DOI co-ordinator, year, ASI has been getting more involved in with the Human Leucocyte Differentiation and the many people involved in organising public advocacy associated with immunology Antigen (HLDA) workshop), with progress these events in our various branches. By related issues. Twice this year, I have teamed reports looking very encouraging. I am all accounts, they were well attended and a up with representatives of other scientifi c also looking forward to an exciting new great success and I have observed fi rst hand societies including Associate Prof. Damian development that we hope to introduce in the the enthusiasm of people in the Melbourne Purcell and Dr Peter Speck (President and 2015 Annual Scientifi c meeting in Canberra area as they planned and participated in Secretary, Australasian Virology Society), (still a secret, but stay tuned …). the Melbourne-based event. Please see the Prof. Paul Young (President, Australian Day of Immunology report in this issue for Society for Microbiology), Associate Prof. Our website http://www.immunology.org.au details. It is a credit to all people involved David Looke (President, Australasian continues to develop and a slightly restyled over the last few years that this has become Society for Infectious Diseases), Clinical version will be launched by the time you read such a popular and successful event that helps Associate Prof. Richard Loh (President, this. I know there have been some teething members get involved in public forum, while Australasian Society of Clinical Immunology problems with this website and as it has simultaneously promoting Immunology to and Allergy), as well as Emeritus Prof. evolved, it was beginning to look a little the general community … well done! Gustav Nossal, to write letters in support of cluttered. This has now been fi xed and I think scientifi cally driven decisions underpinning it is looking very sharp! We will be offering ASI’s journals (Immunology and Cell important public health issues. The fi rst was members the long-promised opportunity to Biology and Clinical and Translational a press release to encourage the Federal enter into a website-based competition to be Immunology, http://www.immunology.org. Government to stand by their promise in the running for an iPad mini, very soon. au/about-asi/journals/) are both doing well. made last year to remove the conscientious Details will follow in an email. I encourage ICB has just received its 2012 impact factor objection option used by up to 7% of families all of our members to visit our new website, of 3.95, maintaining this impressive position in some regions of Australia to bypass the look around, use it to promote stories of while many other Immunology journals vaccination requirements underlying the interest to the Immunology community, are on the decline. Keep submitting your Family Tax Benefi t Part A. The second was a upcoming conferences, job advertisements, articles there and citing studies from there letter to NHMRC applauding their decision to and access websites of our sustaining and we should see this journal climb above investigate the scientifi c evidence underlying members (BD Biosciences, Elisakit.com, an impact factor of 4 next time around. CTI the use of homeopathy for treating important Jomar Bioscience, Miltenyi Biotec). Our is gaining momentum with 16 published health conditions, an investigation that found sustaining members are very valuable to articles and submissions increasing strongly there to be no reliable evidence to support the Society, so please click through to their in 2014. Application for listing in PubMed is the effectiveness of homeopathy. We also websites and look around at what they have underway and article deposition in PubMed proposed that these fi ndings should call into to offer, by clicking on their logos on the Central will be beginning soon, both of question the value of further support for this ASI website. We also encourage you all which should enhance its international profi le particular form of therapy. to use the online renewal process to renew considerably. Please visit the CTI website your membership from now on, although it via the links from the ASI website and We feel that we have more infl uence as is still possible to do this the old way if you have a look at some of the great papers and a collective, representing roughly 4000 prefer. Please contact our Project Manager, reviews being published there. A reminder members of Australasian scientifi c societies, Sarah Fardy, [email protected], with any to all members interested in playing a more and we will continue to represent the members comments or queries regarding the website formal role with these journals – there are of our respective societies on important or online membership renewal. new editorial positions opening up very soon public issues associated with immunology and I will be sending an email with details. and related medical concerns. Please remember that we have the new ASI If you are interested in this, please contact women’s initiative and we are ready to start Gabrielle Belz, [email protected]. Dale Godfrey linking up mentors with mentees, so check out

11 ASI Inc. Newsletter June 2014

HONORARY SECRETARY’S NEWS UPCOMING

The fi rst round of ASI travel awards have institute who are starting out in this fi eld. CONFERENCES been assessed, with congratulations going to Although new members won’t be eligible for the following winners in each category: awards for 2014, they will still get heavily 16th Biennial Meeting of the European discounted registration to the ASI Annual Society for Immunodefi ciencies (ESID Post graduate (up to $3000): Scientifi c Meeting and access to all the 2014) Jason Lynch member-only resources on the redesigned October 29–November 1, 2014 Rachel Bartlett ASI website. Prague, Czech Republic Kylie James www.kenes.com/esid We hope that most of you have now had Post doctoral (up to $3000): a chance to visit the ASI website. The site 44th ASI Annual Meeting Cyril Seillet is funded through your membership and December 1–5, 2014 Alison West provides a great resource for ASI members to Wollongong, NSW, Australia get information about upcoming events and www.asi2014.org Jacques Miller Award for Senior scientists benefi ts such as mentoring programs, travel (up to $6000): awards and a variety of scientifi c resources. If you want to contribute items to the website David Tarlinton that are relevant to ASI members (e.g. news items, notices of upcoming events, etc.), The Walter and Eliza Hall The awards are always highly competitive, please contact Sarah Fardy at fardy.s@wehi. Institute of Medical Research so we encourage ASI members to keep edu.au. Sarah is the ASI Project Manager and WEHI Seminars on the Web: an eye out for the second round of travel is keen to help members post material that www.wehi.edu/seminars/ awards later in the year. The exceptions are keeps the website interesting and relevant so the annual Jacques Miller and Gordon Ada feel free to contact her with suggestions. If awards where members will need to wait until you haven’t seen the website lately, please the start of 2015 before applying. have a fresh look at www.immunology.org. au. And … you will soon see a signifi cant As of May, ASI has 885 fi nancial members. redesign to the website to further improve This is slightly up from the same time last its appearance and functionality, so keep an year and that’s great news given there were eye out for when it goes live (expected to be some early issues with signing up the new the week of June 16) and let us know what The key to understanding the immune system website. Membership is the foundation of the you think of it. is to determine how its proteins, cells and other Society and we ask you to please encourage components interact, at a molecular level, fellow Immunologists to join ASI – especially Stuart Berzins in health and disease. This is what the ARC the junior members of your laboratory or Centre for Advanced Molecular Imaging is all about – developing and using innovative imaging techniques to observe the details of how the immune system functions at the molecular level.

The Centre brings together biologists, physicists and chemists from 5 Australian universities – La Contributions sought for the ASI Newsletter Trobe, Melbourne, Monash, New South Wales and Queensland; the University of Warwick in You could win $200 !! the UK; the Australian Nuclear Science and Technology Organization; synchrotrons in Australia and Germany; and several high-tech Deadline for the next issue: 1st August 2014 companies. Please email your contributions to the Secretariat For more information about the Centre by the above date. participants, research and employment [email protected] opportunities within the Centre, please visit our website and subscribe to our newsletter.

www.imagingcoe.org www.imagingcoe.org/news/category/new sletters

12 ASI Inc. Newsletter June 2014

THE ASI VISITING SPEAKER PROGRAM

The very busy commitments of the high Work of his laboratory aims at understanding Frampton, KJ Liu, F Geissmann. A lineage calibre speakers have affected the VSP the development and physiological functions of myeloid cells independent of Myb and program this year and we had a couple of of monocyte/macrophages in vivo. His hematopoietic stem cells. Science, 2012. unfortunate cancellations: approach is to combine developmental 10.1126. 4. Hambleton S, Salem S, Bustamante J, Bigley studies, lineage tracing, genetics, and intra- John O’Shea MD V, Boisson-Dupuis S, Azevedo J, Fortin vital microscopy to characterize the laws that National Institute of Arthritis and A, Haniffa M, Ceron-Gutierrez L, Bacon govern the development and maintenance of Musculoskeletal and Skin, Molecular CM, Menon G, Trouillet C, McDonald D, phagocytes and interaction with specialized Immunology and Infl ammation Branch, NIH, Carey P, Ginhoux F, Alsina L, Zumwalt tissue cells, within living organisms in mice TJ, Kong XF, Kumararatne D, Butler K, Bethesda, MD. USA. and – more recently – in Drosophila. Hubeau M, Feinberg J, Al-Muhsen S, Cant Hosted by Stuart Tangye, Garvan Institute A, Abel L, Chaussabel D, Doffinger R, of Medical Research, Sydney Frederic Geissmann has made several seminal Talesnik E, Grumach A, Duarte A, Abarca K, Cancelled due to NIH restrictions contributions in this fi eld. He identifi ed and Moraes-Vasconcelos D, Burk D, Berghuis A, Geissmann F*, Collin M, Casanova JL, Gros described the two main functional subsets Jason Cyster, PhD P. IRF8 mutations and human dendritic-cell of monocytes in mice (infl ammatory and Howard Hughes Medical Institute, University immunodefi ciency. (2011) N Engl J Med. patrolling) and their human counterparts of California, San Francisco, CA. USA 365:127-38. (*co-senior author) (Geissmann, Jung & Littman Immunity Hosted by Claudine Bonder, Centre for 5. F. Geissmann, MG. Manz, S Jung, MH. 2003, Auffray et al Science 2007, Cros et al Sieweke, M Merad, K Ley. Development of Cancer Biology, Adelaide Immunity 2010, Carlin, Stamatiades et al monocytes, macrophages and dendritic cells. Postponed for family reasons Cell 2013), identifi ed a common clonogenic Review. Science, 2010. 327:656-61. progenitor for monocyte/macrophages and 6. Cros J, Cagnard N, Woollard K, Patey N, Planned visits Zhang SY, Senechal B, Puel A, Biswas classical dendritic cells (Fogg et al Science We expect to confi rm details for two more SK, Moshous D, Picard C, Jais JP, D’Cruz 2006), and more recently characterised visitors for 2014 D, Casanova JL, Trouillet C, Geissmann an embryonic progenitor independent of F. Human CD14dim monocytes patrol and Myb and hematopoietic stem cells for adult sense nucleic acids and viruses via TLR7 September/October resident macrophages such as Kupffer cells, and TLR8 receptors. (2010) Immunity. Professor Frederic Geissmann 33:375-86. Langerhans cells, microglia, etc. (Schulz et al King’s College, London, UK 7. Chorro L, Sarde A Li M, Chambon P, Malissen Science2012; Gomez Perdiguero et al. Cold Prof. Geissmann will visit Melbourne, B, Kissenpfennig A, Barbaroux JB, Groves R, Spring Harb Symp Quant Biol. 2013; Gomez Sydney, Canberra and Sydney. Geissmann F. Langerhans Cell proliferation Perdiguero et al, In preparation). mediates neonatal development, homeostasis, Details to be fi nalised. and infl ammation-associated expansion of the Hosted by Gabrielle Belz, WEHI Frederic Geissmann was the recipient of a epidermal LC network. J. Exp. Med. 2009. number of prestigious awards, including the 206:3089-100. European Young Investigator (Euryi) award, 8. Auffray C, Sieweke MH, & Geissmann F. Blood Monocytes: Development, the European Research Council Investigator Heterogeneity, and Relationship with award and the Wellcome Trust Senior Dendritic Cells. Annu Rev Immunol.Review. Investigator award. He is currently Professor, 2009. 27:669-92. ARUK chair of Infl ammation biology, and 9. C Auffray, D Fogg, M Garfa, G Elain, O Join- Director of the Centre for Cellular and Lambert, S Kayal, SSarnacki, A Cumano, Molecular Biology of Infl ammation Biology, G Lauvau, & F Geissmann. Monitoring of at King’s College London, UK. blood vessels and tissues by a population of monocytes with patrolling behavior. Science Selected key papers 2007. 317:666-670. 1. Carlin LM, Stamatiades EG, Auffray C, Hanna 10. Fogg DK, Sibon C, Miled C, Jung S, RN, Glover L, Vizcay-Barrena G, Hedrick Aucouturier P, Littman DR, Cumano A, CC, Cook HT, Diebold S, Geissmann F. Geissmann F. A clonogenic bone marrow Nr4a1-dependent Ly6C(low) monocytes progenitor specific for macrophages and monitor endothelial cells and orchestrate their dendritic cells. Science. 2006. 311:83-7. 11. Geissmann, S Jung, DR Littman. Blood disposal. Cell. 2013 Apr 11:153(2)362-75. monocytes consist of two principal subsets 2. Carlin LM, Stamatiades EG, Auffray C, with distinct migratory properties. F (2003) Hanna RN, Glover L, Vizcay-Barrena Immunity 19, 71-82. G, Hedrick CC, Cook HT, Diebold S & Geissmann F. Nr4a1-dependent Ly6Clow Professor Frederic Geissmann gained a monocytes monitor endothelial cells and A/Prof. Anand Goldrath medical degree in clinical haematology in orchestrate their disposal in presence of Section of Molecular Biology, University of 1996 and a PhD in Immunology in 1999, nucleic acids. (2013) Cell. 153: 362–375. California, San Diego, CA. USA. both from the University of Paris (France), 3. C Schulz, E Gomez Perdiguero, L Chorro, (Detailed schedule to be confi rmed) and was a Post-doctoral Fellow of Dr Dan H Szabo-Rogers, N Cagnard, K Kierdoff, M Hosted by Roslyn Kemp, University of Prinz, B Wu, SE W Jacobsen, JW Pollard, J Littman in New York from 2000 to 2003. Otago.

13 ASI Inc. Newsletter June 2014

2015 effector-like resident memory T cells in tissues. We have two speakers confi rmed for the fi rst 2012. J Immunol. 188:4866-75. semester 2015: 33 citations 9. Anderson KG, Sung H, Skon CN, Lefrancois L, April Deisinger A, Vezys V, Masopust D. Cutting Edge: Associate Professor David Masopust Intravascular staining redefi nes lung CD8 T cell University of Minnesota, Department of responses. 2012. J Immunol. 189:2702-6. Microbiology, Minneapolis, Minnesota, 10. Schenkel, JM, Fraser KA, Vezys V, Masopust USA D. Sensing and alarm function of resident memory CD8+ T cells. 2013. Nature Immunology. 14:509-13. A/Prof. Masopust has confi rmed he will visit 11. Masopust D, Schenkel JM. 2013. The NSW, NZ, Qld and Vic. integration of T cell migration, differentiation Hosted by Thomas Gebhardt, Department of and function. Nature Reviews Immunology. Microbiology and Immunology, University 13:309-20. of Melbourne 12. Fraser KA, Schenkel JM, Jameson SC, Vezys V, Masopust D. Preexisting high frequencies David Masopust helped to defi ne a new of memory CD8+ T cells favor rapid memory paradigm in immunology with high impact differentiation and preservation of proliferative potential upon boosting. 2013. Immunity. 39:171- for developing vaccines against important 83. pathogens such as HIV-1 and TB: Tissue resident memory T cells, positioned in April/May 2015 time and space, and suffi cient in number, to Assoc. Professor David Masopust Professor Daniel Altmann prevent or control infections from their onset. Imperial College, London, UK Dr Masopust’s initial discovery underpinning Masopust D, Kaech SM, Antia R, von Andrian Hosted by Nattkunam Ketheesan, James this concept was that memory T cells actively UH, Ahmed R. Lineage relationship and protective Cook University, Townsville survey tissues and mucosal surfaces of the immunity of memory CD8 T cell subsets. 2003. Prof. Altman has confi rmed he will visit Nat Immunol. 4:225-34. body where they are most likely to encounter Sydney, Brisbane and Townsville. pathogens. His technological expertise and >1250 citations 3. Masopust D, Vezys,V, Usherwood EJ, Cauley innovativeness provided the supporting and Full details of Professor Altman’s visit will convincing rigorous scientifi c evidence for LS, Olson S, Marzo AL, Ward RL, Woodland DL, and Lefrancois L. Activated primary and be provided in the September newsletter. this tissue resident host defense system and, memory CD8 T cells migrate to nonlymphoid in work of fundamental importance, he has tissues regardless of site of activation or tissue of explored interactions between the tissue origin. 2004. J Immunol. 172:4875-82. microenvironment and resident T cells to >200 citations help defi ne a new fi eld in immunology. 4. Barber DL, Wherry EJ, Masopust D, Zhu In work with potentially great impact, he B, Allison JP, Sharpe AH, Freeman GJ, Ahmed has discovered a way to vaccinate that R. Restoring function in exhausted CD8 T cells during chronic viral infection. 2006. Nature. elicits extraordinary numbers of these An invitation and a request resident tissue T cells, located where they 439:682-87. >1500 citations to all ASI members fi rst encounter a pathogen, at a time when 5. Masopust D, Vezys V, Wherry EJ, Barber DL, to contribute copy that they think the small size of the infected population Ahmed R. Cutting edge: gut microenvironment might be interesting, useful, historical, provides the most favorable odds to prevent promotes differentiation of a unique memory CD8 humorous or thought provoking. transmission and systemic infection. In a T cell population. 2006. J Immunol. 176:2079- proof-of-principle experiment in the SIV- 83. We invite our student membership monkey model of HIV-1 transmission to >125 citations to voice their views on issues women, he has shown that this approach 6. Vezys V, Yates A, Casey KA, Lanier G, Ahmed that interest or directly concern can prevent transmission or greatly attenuate R, Antia R, Masopust D. Size of memory CD8 them. systemic infection, a result without precedent T cell compartment grows with immunological experience. 2009. Nature. 457:196-9. in the HIV-1 vaccine fi eld, and one that holds It’s our newsletter, so let’s >100 citations great promise for developing an effective support it and strive to make it 7. Masopust D, Choo D, Vezys V, Wherry EJ, even better. vaccine. Duraiswamy J, Akondy RS, Wang J, Casey KA, Barber DL, Fraser KA, Kawamura KS, Webby RJ, The ASI newsletter comes out Selected key publications: Brinkmann V, Butcher EC, Newell KA, Ahmed 4 times a year and we welcome 1. Masopust D, Vezys V, Marzo AL, Lefrancois R. Dynamic T cell migration program establishes L. Preferential localization of effector memory resident memory within intestinal epithelium. your contributions. cells in nonlymphoid tissue. 2001. Science. 2010. J Exp Med, 207:553-64. 291:2413-7. 96 citations AND YOU COULD WIN >1350 citations. Recently designated a “Pillar 8. Casey KA, Fraser KA, Schenkel JM, Moran A, $200 FOR THE BEST of Immunology” by the American Association Abt MC, Beura LK, Lucas PJ, Artis D, Wherry ARTICLE PUBLISHED IN of Immunologists. EJ, Hogquist K, Vezys V, Masopust D. Antigen THE NEWSLETTER! 2. Wherry EJ, Teichgraber V, Becker TC, independent differentiation and maintenance of

14 ASI Inc. Newsletter June 2014

Treg Cells

Regulatory T Cells Keeping the Immune System in Check

Antibodies pSTAT5 (h, m) New CD304 (Neuropilin-1) (h, m) New AHR (h, m) New c-Maf (h, m) New Helios (h, m) New ICOS (h, m, r) New Eos (m) New GARP (h, m) New

ELISA Kits IL-10 ELISA Ready-SET-Go!® (h, m) New ProcartaPlex™ Multiplex Immunoassays IL-2 Simplex (h, m, r, nhp, c) New IL-10 Simplex (h, m, r, nhp, c, p) New TGFβ1 Simplex (h, m, r, nhp, c, p) New Th9/Th17/Th22/Treg Cytokine Panels (h, m) New Th1/Th2/Th9/Th17/Th22/Treg Chemokine Panels (h, m) New Recombinant Proteins TGFβ1 Recombinant Protein (m) New SAFE™ IL-2 Recombinant Protein (h) New

h=human, m=mouse, r=rat, nhp= non-human primate, c= canine, p=porcine

ϭϯϬϬϲϲϱϮϳϯ T regulatory (Treg) cells are CD4+ T cells that are critical to the ũŽŵĂƌΛĂĚĞůĂŝĚĞ͘ŽŶ͘ŶĞƚ maintenance of immune cell homeostasis as evidenced by the catastrophic consequences of genetic or physical ablation of ǁǁǁ͘ĞďŝŽƐĐŝĞŶĐĞ͘ĐŽŵ the Treg population. Specifically, Treg cells maintain order in the immune system by enforcing a dominant negative regulation over other immune cells. Treg cells are characterized by expression of the Foxp3 transcription factor that specifies differentiation of the lineage.

15 ASI Inc. Newsletter June 2014

Three To One! Susan Christo, University of South Australia

On 12th February, all three South Australian universities participated in the inaugural Tri-University Distinguished Guest Speaker Evening; a night that brought PhD students together for networking and career advice. The sold-out event attracted 160 delegates from The University of Adelaide, The University of South Australia and Flinders University.

The idea The premise was simple. As my lab friend Heather and I sat at a science Gala Dinner, she turns to me and says: “I was thinking, we should have a career’s night for the students, something like [this]. We could have a sit-down event with food, have some Nobel Laureate Professor Peter Doherty, AC! Truly offering a fantastic, inspirational and speakers”. Her vision was to bring together entertaining presentation about his remarkable career, juxtaposing advice and the truths PhD students regardless of what university about working in scientiﬁ c research or ﬁ eld of health research they were in. his humble account of his exceedingly stellar Advanced Sensing), Professor Gary Wittert That was all good, but we had $0. So with career. The evening also consisted of a Panel (Director, Freemasons Foundation Centre a six-woman team, we began sourcing Discussion which followed very closely to for Men’s Health), Professor Lynne Cobiac sponsorship, and the original idea began the ABC’s Q&A program, allowing audience (Director, Preventative Health Flagship, to develop. participation and a broad range of feedback CSIRO) and A/Professor Janna Morrison from the panellists. (Head, Early Origins of Adult Health On the night Research Group, UniSA). The highlight of the evening was the keynote We were privileged with outstanding Panellists address given by Laureate Professor Peter including Professor Doherty, Professor The discussion was perfectly complemented Doherty, AC. His presentation was inspiring, Graeme Young (Professor of Global GI with Dr Paul Willis (Director, RiAUS) as entertaining, honest and passionate; yet his Health, Flinders University), Professor Tanya host, who was able to extract sentimental true dedication to the science was evident in Monro (Director, Institute for Photonics and and thought-provoking comments from

Thank you once again to our The Tri-University Panel Speakers (LtoR) Prof. Lynne Cobiac, Prof. Graeme Young, A/Prof. Janna Morrison, Prof. Tanya Monro, Prof. Gary Wittert, Nobel Laureate Peter Doherty, AC, Dr Paul Willis 16 ASI Inc. Newsletter June 2014

Panel Discussion generating plenty of discussion! LtoR: Prof. Gary Wittert, Prof. Lynne Cobiac, Prof. Tanya Monro, Dr Paul Willis, A/Prof. Janna Morrison, Prof. Graeme Young, Prof. Peter Doherty answers and questions raised by the students the need to collaborate and network was often, that naïve question is the very best themselves. The most incidental and, in echoed by all panellists. This was further question”. He makes it sound so easy! But fact, coalescing aspect of the evening was highlighted by Prof. Wittert when discussing certainly not impossible. The message was the overriding theme of collaboration and post-doctoral opportunities: “Networking, clear and simple. networking, which was consistently discussed showing ethusasiam and making friends in as a key characteristic of a successful career. the right places will get you a very long way”. Another popular issue of discussion was The evening was concluded by a fantastic Prof. Young also added to the conversation, the notion of mentors. It was unanimously presentation from Professor Wittert who detailing the valuable experiences gained in agreed that mentors are benefi cial, however elaborated on various aspects of being a overseas trips: “I got to know very quickly only when it’s the right person. Prof. Cobiac successful researcher and offered perspective the people about my age, doing good stuff. pointed out that not all mentors are good of various science career options beyond that Now we’re writing papers together… you’re scientists, and that students should approach of working in a lab. investing for the future”. “mentors that have integrity” in their work. In addition, Prof. Young suggested that “a Don’t worry Peter, we’ll take your advice! But for students, the idea of talking to people good mentor is someone who cares for you To unexpected ears, a presentation by you don’t know and networking can be as a person”. Prof. Doherty is far from orthodox. Prof. frightening. It was refreshing to hear that Doherty has a unique ability to juxtapose the panellists did, at times, feel ‘shy’ and Naturally, the conversation was directed thought-provoking, deep, soulful accounts ‘introverted’. In fact, Prof. Monro outright towards the importance of work/life balance, of immunology with humorous, honest and stated: “I’m a classic introvert, but you’d particularly focussed on maintaining high humble recollections of his life. Prof. Doherty never guess it. You learn”. One comment that scientifi c standard and having a family. played to his vast experience, describing the resonated well with the audience came from This issue is of personal interest to women solid advice he gives his students wanting A/Prof. Morrison, who revealed that the idea considering children during their careers, a career in research: “Find yourself a nice of networking is a very pro-active motion: “I to which Prof. Monro realistically said, accountant, or corporate lawyer, or banker. am very shy. [But when at conferences] my “There’s no magic bullet. [During maternity And marry them! They’ll be able to support options were to stand in the corner, or go talk leave] my PhD students would give me a you. Not a single one of my graduate students to people. I decided, my ‘hat’ that’s now on chapter of their thesis in exchange for a have taken my advice. They’ve always is “I’m talking to people” and that’s what I little bit of babysitting. There are ways you married for love!” have to do”. As students, we often neglect the can make it work”. A/Prof. Morrison also fact the most successful scientists all started contributed, highlighting the advantage of What I really enjoyed about Prof. Doherty’s off at our level, and may, at times, have the research careers: “I tailor the way I work. presentation, and in fact all of the panellists, same uncertainties and doubts. The fl exibility is amazing”. were their genuine care for students. Their advice was real, open, and could be applied in But when attending conferences alone, how Another issue that is often overlooked is the all areas of science. The students particularly do I approach someone? Will I say the wrong need to communicate science to the public, appreciated their honesty about careers in thing? Prof. Doherty’s advice: “Don’t be not only to fellow scientists. I found this research, Prof. Monro admitting, “It can afraid to ask questions and don’t be afraid particularly interesting because we often be a bit overwhelming, at an early career to make a fool of yourself. I’ve been making forget that science is ultimately for the benefi t stage, the things you need to get on your a fool of myself for years! Don’t be afraid of the greater community, regardless of how track record to be competitive”. However to ask the simple naïve question because specifi c and deep into the fi eld the work is.

17 ASI Inc. Newsletter June 2014

The evening’s ambience at the Adelaide Pavilion; Prof. Doherty presenting with our sponsors proudly displayed

However, the issue may be that the public Of course, a big thank you to the hard- university society, highlighting the power aren’t aware of the translational implications working women on the committee who of collaboration for translating a simple idea of research. “We no longer need journalists. tirelessly helped organise the event. Thank into an inspirational evening. Everyone has the capacity to communicate you to Dannielle Post (co-representative science on a broad basis,” Prof. Wittert states. from UniSA), Heather Armstrong and For more information and photos, please visit It certainly made the audience consider the Emma Stewart (University of Adelaide), our website http://triuni2014.weebly.com/ social responsibility of a scientist, as Prof. Karen Patterson and Nuy Chau (Flinders or please feel free to contact me at susan. Cobiac perfectly states: “We need to have a Unviersity). This event was organised [email protected]. convincing story of how we can demonstrate and fully funded independent of any impact”. This was a particularly motivating comment for me because, like many students, I lose sight of how the work I do on a day-today basis will integrate into society. I hope the Tri-University Dinner was able to inspire the delegates to appreciate the impact their work will have.

The panellists are model examples of how hard work and exceptional science can be recognised in the greater community. We thank all the guests of the evening for their time, experiences, and for sharing their advice to inspire the future scientists of this generation.

Onwards and upwards The union between the University of South Australia, University of Adelaide and Flinders University, has not been previously achieved in a manner such as the Tri- University Dinner. It lacked the formality and heavy scientiﬁ c content of conferences, and brought with it an enjoyable night for students to learn about all aspects of ‘being Tri-University Committee members (LtoR) Karen Patterson (Flinders University), Nuy Chau a researcher’, not just ‘doing research’. (Flinders University), Susan Christo (University of South Australia), Heather Armstrong (University of Adelaide) and Emma Stewart (University of Adelaide). \ Absent: Dannielle Post (University of South Australia co-representative)

18 ASI Inc. Newsletter June 2014

19 ASI Inc. Newsletter June 2014

Day of Immunology 2014 in Victoria Claerwen Jones Chair, Day of Immunology Melbourne Organising Committee Over 600 members of the general public and to Marvel Comics characters!), and how Award-winning Australian documentary secondary students participated in another vaccines have developed over the years fi lm maker gave a fabulous and passionate excellent series of Day of Immunology (DoI) through to the modern age. Did you know that talk about her fi lm Jabbed: Love, Fear and events in Victoria in 2014. Many thanks to one of the Incredible Hulk’s super powers is Vaccines that explores the benefi ts and risks the sponsors, the fabulous DoI committee, immunity to diseases and viruses? of vaccination. We were very fortunate to Communications staff at WEHI and Monash work with Wendy Reid from Inner North University, Medicare Local staff, GTAC Dr Krystal Evans (Walter and Eliza Hall West Melbourne Medicare Local, who staff, and speakers, tour guides and helpers Institute of Medical Research) gave a great performed the vaccinations and Anne for making DoI 2014 such a success. talk on the very old problem of malaria, McGlashan from University of Melbourne diffi culties in developing a vaccine to this Health Service who organized the vaccines Public Lecture: Visions for a disease-free pathogen and three exciting new vaccine and equipment. Thanks also to Thomas world: Vaccinations against infectious strategies developed at WEHI that are Gebhardt and Sammy Bedoui (UniMelb) and diseases currently in clinical development. Su Heinzel (WEHI) for expert advice and all An appreciative audience enjoyed an the researchers who presented fabulous lay excellent series of lectures by four Melbourne Prof. Nigel Curtis (University of Melbourne posters about their work (Nick Collins, Marie scientists on how vaccination helps to protect and Royal Children’s Hospital Melbourne) Greyer, Jyh Liang Hor, Alex Kato, Jane Li, us from infectious diseases. gave a very entertaining presentation about Kylie Quinn, Ali Zaid). Congratulations to whether “to BCG or not to BCG”, and how poster winner Dr Daniel Fernandez-Ruiz Dr Irina Caminschi (Chairperson, Burnet childhood BCG vaccination is showing (UniMelb). Institute) gave a wonderful opening potential to reduce allergies and boost the presentation on vaccination from the immune response to other infections. Discovery tours perspective of an immunologist and a Over 200 people (including members of the mother, dispelling several commonly held Vaccination Café general public, nursing, uni or school students misconceptions on vaccination safety. This was a new activity for DoI Victoria. and teachers) attended a Discovery Tour Over 30 members of the general public came held at either the Monash Health Translation Prof. Stephen Turner (University of to get their infl uenza vaccination for only Precinct, Ludwig Institute for Cancer Melbourne and Doherty Institute) spoke $10 and/or chat with research scientists over Research, Burnet Institute, Peter MacCallum about the history of vaccination, how tea, coffee and biscuits about the science of Cancer Centre or CSIRO’s Australian vaccination actually works to protect against vaccination and how the body fi ghts illness Animal Health Laboratory. Tours included disease (relating immune cell functions and disease. Sonya Pemberton, 2012 Emmy talks by PhD students and postdocs, poster viewings, tours of laboratories and facilities and the chance to talk with researchers over refreshments.

Participants and organizers alike had a really positive experience and there was heaps of great feedback, including “Thank you so much for your time yesterday. I found the day very informative and fascinating; how far research has come over the last few years is amazing. What a delightful group of young researchers.”

Thanks to Jim Harris, Paul Hertzog, Eric Morand, Marcel Nold, Ashley Mansell, Alison Browning, Camden Lo, Ina Rudloff and Vivian Vasic, and other volunteers from MHTP; Katherine Woods and volunteers from Ludwig Institute; Kerry Ko, Rachel DoI 2014 Committee Lundie, Stephanie Luketic, Bruce Loveland, LtoR Front row: Dimmy Zotos, Tim Johanson, Claerwen Jones, Laura Mackay, Aislin Meehan. Ben Fancke, Xi Zen Yap, Tom Angelovich, Back row: Alison West, Nicole Messina, Wy Ching Ng, Connie Duong, Jay Rautela, Rachel Brendan Elsworth, Jun Gu, Elisha de Valle, Lundie. Jess Li, Jess Anania and Andrew Guy from (Photo: Gayle Davey, UniMelb) Burnet Institute; Connie Duong, Jessica Absent: Ann Cornish, Erika Duan, Jim Harris, Gabi Khoury, Kerry Ko, Julia Marchingo, Scott Salmon, Nicole Milenkovski and Caroline Mueller, Kim Pham, Azad Rahimpour, Lucie Rankin, Louise Rowntree, Linda Wakim, Clare Owen from Peter Mac; and Daniel Layton, Westhorpe, Wendy Winnall, Katherine Woods. 20 ASI Inc. Newsletter June 2014

Sonya Pemberton speaking at the Vaccination Café (Photo: Jeremy Kennett, Inner North West Melbourne Medicare Local)

Public lecture presenters . LtoR: Steve Turner, Irene Caminschi, Krystal Evans & Nigel Curtis Adam Karpala and Lenny Izzard and (Photo: Susanne Heinzel, WEHI) volunteers from AAHL/CSIRO.

Immunology workshops Nearly 300 VCE Biology students and their teachers from 30 Victorian secondary schools attended one of three full day workshops at either the Gene Technology Access Centre (GTAC) in Parkville or Federation University, Ballarat. The workshop began with talks by eminent immunologists, including Sir Gustav Nossal, Prof Phil Hodgkin (WEHI) and Prof Paul Hertzog (Monash University) and was followed by various activities (microscopy, ELISA and a GTAC-developed online immunology game) to give the students a strong foundation in the Immunology component of their Biology course. The program ﬁ nished with inspiring careers talks by scientists, including A/Prof. Eugene Maraskovsky (CSL), Dr Julianne Bayliss (Victorian Infectious Diseases Reference Laboratory), Dr Jason Tye-Din (WEHI), Dr Misty Jenkins (Peter Mac) and Prof. George Kannourakis (Ballarat Oncology & Haematology). Feedback comments from students and teachers were, once again, extremely positive.

Many thanks also to Dr Tony Chiovitti, Nicole Webster and all the support staff at GTAC and A/Prof. Stuart Berzins and his Dimmy Zotos (WEHI) inviting passers by to get their ﬂ u shot at the Vaccination Café. support staff at Federation University. (Photo: Jeremy Kennett, Inner North West Melbourne Medicare Local) The Committee would like to gratefully acknowledge the generous support from the following organizations: Australasian

21 ASI Inc. Newsletter June 2014

Society for Immunology, Australian Academy of Science, Australian Biosearch, BD, Bioline, Bio-Rad, Burnet Institute, CSIRO, CSL, Federation University, Gene Technology Access Centre, Immunology Group of Victoria, Interpath Services, Ludwig Institute, Medicare Local Inner North West Melbourne, Miltenyi Biotec, Monash Health Translation Precinct, Monash University, Peter MacCallum Cancer Centre, Sigma-Aldrich, Stemcell Technologies, Taltarni Vineyards, The University of Melbourne, The University of Melbourne Faculty of Medicine, Dentistry and Health Sciences Infection & Immunity Research Domain, and the Walter and Eliza Hall Institute of Medical Research. Professor Sir Gustav Nossal with students from Clonard College and their teacher, Sam Whitton (Photo: Monash University)

Prof. Phil Hodgkin (WEHI) speaking about the Careers speakers Eugene Maraskovsky (CSL), Julianne Bayliss (Victorian Infectious Diseases history of immunology research in Australia Reference Laboratory) & Jason Tye-Din (WEHI) (Photo: Monash University) (Photo: Monash University)

PhD student Erika Duan (Monash University) demonstrating to secondary students in the microscopy workshop (Photo: Monash University)

22 ASI Inc. Newsletter June 2014

Local VCE students from the Ballarat region attending the DoI Immunology workshop at Federation University (Photo: Federation University)

PhD student Jason Lao, Dir Ina Rudloff and nursing students looking at cells at the Monash Health Translation Precinct Discovery Tour (Photo: Monash University)

An aspiring young scientist counting live cells on a haemocytometer, during the Burnet Institute Discovery Tour (Photo: Burnet Institute)

23 ASI Inc. Newsletter June 2014

DIAMANTINA INSTITUTE “turning scientific discoveries into better treatments”

Advanced Immunology Course at The University of Queensland Diamantina Institute Win a $3,200 stipend towards your Honours degree

The University of Queensland Diamantina Institute is pleased to offer a Winter course in Advanced Immunology at the new Translational Research Institute in Brisbane.

Five day course from > All-day attendees are eligible to win 14th - 18th of July, 2014 $3200 Honours scholarship stipend, registration to BIG (Brisbane The Advanced Immunology Course will Immunology Group) and a year offer students: subscription to ASI > Advanced instruction in immunology Costs > Advanced practical training in > All-day attendees (interactive lecture immunological techniques series + Practicals) is $210 + GST > Meet and hear from international leaders (numbers limited) in the ﬁeld of immunology, including > Interactive lecture attendees Professor Robert Brink (Garvan Institute (morning session only) is $60 + GST for Medical Research), Professor Christian Engwerda (QIMR), Associate Professor Five bursaries will be offered based on Kristen Radford (MRI-UQ) and Associate academic record, and a 150 word essay Professor Ray Steptoe (UQDI) on your interest in immunology.

Applications Please submit an application form and pay for your course by Friday June 20, 2014. Applications can be found at - www.di.uq.edu.au/advanced-immunology-course

For further information, please contact Dr Bruce Wyse on (07) 3443 7020 or email [email protected]

Conditions: A busary of $3,200 will be awarded to the winning student who successfully completes the Advanced Immunology Course. The winner will be determined by the course leader. The stipend must go towards an Honours degree at UQDI and cannot be used for any other purposes.

24 ASI Inc. Newsletter June 2014

ASI COUNCILLORS’ NEWS

N.Z. News the bugs had NOT won and the immune Meanwhile, preparations continue for the system was doing its job (but it was very NZASI meeting in July in Palmerston North This year, we have been fortunate to host close!). The debate was held on 5 May 2014 (chair: Jo Roberts). We have three excellent Peter Andersen (as part of the Visiting at Rutherford House, Victoria University of invited speakers to support our unifying Speaker Program) who gave talks in both Wellington. Our courageous debating team theme of ‘One Health’: Dunedin and Palmerston North, as well as members were: meeting with staff and postgraduate students. Leon Knippels, Utrecht University, Students in particular had time for stimulating Afﬁ rmative Team: Dr Laura Green (captain), Netherlands: Immune Modulation by Dietary discussions around their science and their Varun Venkatesh, Nonny, Darian, and Tom Compounds future careers. Negative Team: Dr Lieke van den Elsen, Amy Jane Oliaro, Peter MacCallum Cancer Shepherd, and Gillian McNaughton Centre, Australia: Lymphocyte Signaling, We also held two Day of Immunology events Immune Cell Differentiation – a quiz night in Dunedin, open to the public, Dunedin (Jo Kirman): In Dunedin, we Martin Vordermeier, Animal Health and and a debate in Wellington. celebrated International Day of Immunology Vet. Lab. Agency: Veterinary Immunology with a ‘Plagues and Pestilence’ public quiz and TB Research. Wellington (Anne LaFlamme): In night. We had 12 teams participate and they Wellington, the World Day of Immunology were presented with a multitude of facts about Below are some photos from the Dunedin was celebrated by rigorous debating of immunology and immunology research at quiz night and Wellington debate for Day the moot: “The bugs have won!” The two Otago University before they were tested on of Immunology. debating teams were comprised of senior loosely related general knowledge questions. scientists, PhD students and secondary A highlight was the explanation of the Y shape Roslyn Kemp school students, who spent the month of of antibodies followed by a question about Councillor April preparing to battle for the supremacy the origin of Y-front undies, as well as the of bacteria or the immune system. While bonus round listing different types of tractors both teams fought valiantly, in the end, (it’s New Zealand!). The joint winners of the the audience, who ranged from primary best team names were: “Ring O’ Roses” and school students to pensioners, decided that “Pocket full of Posies”.

Dunedin Quiz Night Top pic shows quizmasters Liam Brennan & Jo Kirman (partially hidden)

Wellington Debate Left: The two teams with Anne LaFlamme

Below: The Afﬁ rmative Team

25 ASI Inc. Newsletter June 2014

Queensland News Dr McElrath also led discussion amongst Victorian News interested PhD students from south east The Queensland ASI Branch has had a Queensland and met one-on-one with some busy month. World Day of Immunology local immunologists. She left us with a was celebrated in style and my thanks go standing invitation to visit her in Seattle, In Autumn, IgV presented the Annual to Danielle Stanisic and Jennifer Reiman while commenting that she was sure that none Master Class in Immunology drawing on a for organising a fantastic series of public of us would be able to tear ourselves away fantastic line-up of local experts covering a lectures in Brisbane, and to Margaret Jordan from the pleasures of Brisbane in general and range of immunological research topics and and Tammy Dougan who organised a highly the new TRI building speciﬁ cally. breakthrough new technologies. Professor successful event in Townsville. Thanks also Frederica Sallusto’s presentation on human to Ian Frazer and TRI who hosted Dr Juliane Report on Brisbane Day of Immunology TCR diversity in effector T cell populations McElrath as part of the ASI Visiting Speaker Event capped off a great day that was very well Program. More details on these events can Danielle Stanisic attended by students and postdoctoral be found below. fellows. A number of events were organised On Sunday 4th May 2014, iQ held its second to celebrate the Day of Immunology in The 15th Brisbane Immunology Group Day of Immunology event at the Queensland Melbourne, Ballarat and Geelong. The new Retreat will be held at Mantra Legends Hotel, Museum. Entitled “Our Amazing Immune Vaccination Café and public lectures were Surfers Paradise from 21–22 August. We System”, it was a public event designed to extremely successful and an article from Dr have an exciting line-up of interstate speakers educate and fascinate people about different Claewen Jones in this newsletter provides including Prof. Liz Hartland and A/Prof. aspects of the immune system with six more details of the Day. Congratulations Katherine Kedzierska from the University engaging sessions from local speakers on to everyone involved in organising these of Melbourne, Prof. Carola Vinuesa and topics including: Diabetes, Rheumatoid events. A/Prof. Ian Cockburn from ANU, Dr Cindy Arthritis, Multiple Sclerosis, Cancer, Ma from Garvan and A/Prof. John Silke Vaccination and cells of the Innate Immune Another ﬁ xture on the IgV calendar looms from WEHI. See the BIG website www.big. System. We would like to thank Prof. on July 25 – the Annual IgV Winter Seminar. qimrberghofer.edu.au/page/Annual_Retreat John Aaskov, Prof. Mike McGuckin, Prof. This year, Professor Antonio Lanzavecchia to register and submit your abstract before Ranjeny Thomas, Dr Gary Allen, Dr Adam delivers the seminar at The Castle Hotel, July 11 2014. Wall and Dr Chris Schmidt for presenting with the support of long-term sponsors and discussing their research and experiences Miltenyi Biotec helping to ensure a great The Queensland Branch is sponsoring and generating a lot of interest and discussion night. The salubrious setting is sure to make an Advanced Immunology Course being amongst the attendees and speakers. for a stimulating seminar. Please feel free to organised by the University of Queensland contact me about these events, or any other Diamantina Institute at the Translational matters relating to Victorian and Tasmanian Research Institute from July 14–18, 2014. ASI activities. More details can be found at www.di.uq.edu. au/advanced-immunology-course Daniel Gray Councillor Kristen Radford Councillor

Report on visit by Dr Juliane McElrath Ian Frazer

Queensland ASI and the new Translational Research Institute on the Princess Alexandra Sustaining Hospital campus were delighted to host Dr Membership Julie McElrath from the Hutch in Seattle as our ASI visitor in May. Dr McElrath, during ASI Inc acknowledges the a 20 hour visit, had dinner with several local support of the following immunologists, and then spent a day at the sustaining members: Institute where she gave an informative Speaker Dr Gary Allen and thought provoking lecture on the many • BD Biosciences reasons why we can’t assess the efﬁ cacy of Report on Townsville Day of Immunology * Centre for Advanced HIV vaccines except through clinical trials, Event Molecular Imaging and an update on the state of those trials Margaret Jordan and Tammy Dougan • ELISAkit.com worldwide. There was heated discussion • Jomar Bioscience amongst the immunologists and vaccine The Day of Immunology was celebrated on • Miltenyi Biotec Australia developers who were present amongst 1st May in Townsville, organised by two of the ~100 audience about the implications the Queensland branch committee members, for other vaccines under development. Dr Margaret Jordan and Ms Tammy Dougan. The event was well attended by 26 ASI Inc. Newsletter June 2014

Two views of the Townsville DoI event

Brisbane DoI Speaker Dr Adam Wall approximately 120 members of the general public. Participants enjoyed a glass of wine or bubbly or a beer or soft drink accompanied by nibbles of cheese, biscuits and fruit, while browsing the many posters showing aspects of the immune system and its functions. An interactive ITC was available for those wishing to “Learn immunology in 5 mins”, and the CDs and booklets on “Our incredible immune system” and “Vaccinations” went down a treat.

This open session was followed by four A.C.T. News S.A./N.T. News informative talks covering different topics To celebrate World Day of Immunology we On Saturday, May 3rd, the SA/NT branch of of how incredible our immune system is. organized public lectures by Professor Carola ASI teamed up the South Australian Health Tammy Dougan opened the session by giving Vinuesa and Professor Mathew Cook about and Medical Research Institute (SAHMRI) a short overview of immunology, which the application of new genomics technologies to celebrate the Day of Immunology. The was then followed by topics on allergies to diagnose and investigate immune mediated state-of-the-art new SAHMRI building and autoimmunity, addressing “Can Your diseases. At the same time, a new Centre for made a stunning venue for this year’s event, Food Kill You?” (A/Prof. Andreas Lopata) Personalised Immunology (jcsmr.anu.edu. “Immunology: Improving Modern Society”. and “Worms! A Cure For Infl ammatory au/research/cpi) was launched by Assistant Members of the public were invited into Diseases?” (Dr Paul Giacomin). A/Prof. Health Minister Fiona Nash. The event was the foyer for an interactive display which Patrick Schaeffer wrapped up the session with very well attended with more than 180 people featured posters, a microscope station as well a talk on how to “Help fi ght Melioidosis”. coming to the lectures and also received a as immunology-based educational games and lot of media attention through an ABC story activities for all ages. The floor was then opened to general and a media release from the Department questions that were fi elded by our experts. of Health. We were lucky enough to host three The talks were most enjoyable and there was engaging speakers to deliver public great cohesion due to the talent of our MC, Together with the NSW branch, we are lectures on their areas of interest, Prof. Prof. Alan Baxter. In order to get our students looking to another 2-day retreat on Thursday Eric Gowans (Vaccines), Assoc. Prof. involved, we had a poster prize for the best 28th and Friday 29th August 2014. As in the Bob Heddle (Allergy) and Prof. Sarah student poster of the evening. This was won previous four years, the retreat will be held Robertson (Reproductive Immunology). The by Tiange {Susan) Lui, a PhD student from at Peppers Craigieburn Conference Centre audience was then given the opportunity to the Medical Genomics Group at JCU. and Resort in Bowral. We are very excited ask questions in panel discussion with the to have Lynn Corcoran (WEHI), Wolfgang speakers. Following the panel discussion the Due to the enormous success of this event, we Weninger (Centenary Institute), Bernadette interactive display was open for the public will endeavour to have a repeat performance Saunders (Centenary Institute) and Thomas to wander around and ask questions. A well- next year. We welcome any interested parties Gebhard (University of Melbourne) as received attraction of the display was an iPad who would like to participate in giving a invited keynote speakers. station with the ‘POX’ game. The aim of this presentation to contact us (Margaret.Jrdan@ educational game is to stop the spread of a jcu.edu.au; [email protected]). Anselm Enders deadly infectious disease and it encourages Councillor players to learn the importance of group immunity and the need to vaccinate. The event was well attended by the public who

27 ASI Inc. Newsletter June 2014 were interested to ask questions and learn SA/NT Branch DoI event, “Immunology: Improving Modern Society”, more about the immune system. held at the new SAHMRI building

A big thankyou to our invited speakers, Prof. Eric Gowans, Assoc. Prof. Bob Heddle and Prof. Sarah Robertson who gave up their Saturday to come along and share their passion for immunology and to our sponsors SA Pathology and UniSA. I would also like to thank the organising committee, who helped in the lead up to the event as well as on the day, I really appreciate all of your help in making the event a success: Susan Christo, Erin Lousberg, Natalie Stevens, Anita Kral, Natalie Aboustate, Iain Comerford, Pallave Dasari, Lisa Ebert, Tessa Gargett, Natasha Kolesnikoff, Shamika Moore, Gaurav Singhal, Peter Speck, Houng Taing. Poster Display

Our next big ASI event for SA/NT will be the 10th Annual Adelaide Immunology Retreat (AIR-10). It has been great to watch the success of the event grow over the past 10 years and hopefully this year we can continue that trend! The retreat, which is aimed at giving PhD students, Honours students and Research Assistants the opportunity to present their work and interact in a relaxed environment, will be held in August this year. An advertisement of the exact date and call for abstracts will be sent out by email to all SA/NT ASI members in June. Please support this event if you are a supervisor Pox game at the vaccination station in the interactive display by encouraging your students and staff to attend as it is a great opportunity for them to give an oral presentation to their peers in a relaxed environment. Another incentive is that there are prizes for the Best Presentations. For queries or more information, I can be contacted by email at Cara.Fraser@sahmri. com. We look forward to seeing you there!

Cara Fraser Councillor

Prof. Eric Gowans delivering his public lecture on vaccination

Panel Discussion

28 ASI Inc. Newsletter June 2014

TRAVEL AWARD CONFERENCE REPORTS

The Non-Coding Genome Symposium, EMBL 9-12 October, 2013, Heidelberg, Germany Monika Srivastava The John Curtin School of Medical Research, ANU, Canberra

While working on microRNAs during my (lncRNAs) and microRNAs (miRNAs). I have had the opportunity to make some PhD project, I always wished that one day I This was very helpful as the fi eld of non- great friends there and hope to remain in would be able to meet some of the pioneers in coding RNA is still in its naive state with a contact with them in some way, shape or this fi eld. This wish came true when I received lot of potential for exciting discoveries. The form. The conference also provided an the Postgraduate International Travel Award major problems faced by researchers today opportunity for some fruitful networking for from ASI to attend the non-coding genome in the fi eld of microRNA is the availability scientifi c collaboration. I am very hopeful symposium this year in Germany. Being my of suffi cient techniques and this workshop that these collaborations will not only benefi t fi rst international conference, I was very provided us with some of the options which the ongoing projects but also for upcoming excited to travel abroad and equally thrilled to was quite reassuring. After the workshop, projects on miRNAs. The conference also meet and talk to some of the eminent scientists the symposium started with the opening showcased the use of advance technologies in the fi eld of microRNA. The conference was talk by Prof. Elisa Izaurralde. She told that like RIP-Seq, deep sequencing, ribosomal held in EMBL, Heidelberg which itself is a the symposium had 477 registrants and 300 profi ling in studying expression, function very popular destination among molecular posters from all across the world, highlighting and homeostasis of non-coding RNAs. biologists. The interior of the building was the success of the symposium. The next talk quite interesting with the staircase running was from emeritus scientist Prof. Witold Although the weather was not very tourist in a double helix of DNA. The two sides of Flipowicz, and it was such a pleasure to friendly, still I took advantage of being in the the stairs, or the two strands we should say, hear him and know more about the Ccr4-Not city and looked around the beautiful town. were interconnected just like a DNA strand protein that forms the core components of It was a pity that due to family reasons, I that often created lot of confusion between post-transcriptional regulation of mRNA. could not take some extra days other than the outsiders. In fact, I ended up twice on the The four days of comprehensive coverage the conference to visit some more countries wrong fl oor due to the complicated, rather of non-coding genome was very informative of Europe, but was extremely happy with my interesting design of the stairs. and exciting. This meeting not only provided overall experience. a chance to listen to some wonderful work Prior to the start of the symposium, there but also gave me the opportunity to meet one- Once again I would like to thank the ASI for was a short workshop hosted by Exiqon to-one with some of the eminent scientists in providing me this travel grant and giving that told us about the recent advancement the fi eld of microRNA such as David Bartel, me the opportunity to explore the outside and latest techniques applied to study non V Narry Kim, Witold Flipowicz, and Elisa scientifi c world, with may be some potential coding RNAs such as long non coding RNAs Izaurralde. postdoc employers.

29 ASI Inc. Newsletter June 2014

15th International Congress of Immunology August 22-27, 2013, Milan, Italy Susan Christo Hanson Institute, Adelaide, SA

Fashion is to Milan as immunology was to ICI 2013. The Pradas, the Guccis and the Versaces of the immunology world were welcomed into Milan’s congress centre, united by a common passion of science. It was overwhelming to be immersed in an environment so concentrated with the world’s top researchers. The intellectual experience was invaluable; I had the opportunity to listen to presentations by Peter Doherty, Mark Davis, Rino Rappuoli, Alberto Mantovani, Vishva Dixit and many more.

What seemed to be a somewhat minuscule contribution to the conference (it was like walking through a real-life hall of fame!!), my poster presentation was one of the most rewarding experiences of my career to date. It was exciting to present my work to an international audience gathered around, and in particular, voice my struggles/ troubleshooting adventures with students who had the same experiences! Susan visiting the ICI 2016 booth at ICI 2013, with an unexpected visit from Australian wildlife! The European experience also accommodated a lab visit with Professor Ed Palmer in Basel, IGSIC conference (Marseille, France) aimed I want to express my gratitude to ASI Switzerland, who generously donated his for international immunology students. for the ICI2013 award that supported my time and knowledge with me. I really enjoyed Amongst the world-wide representation at European endeavours. gaining more insight into how scientists from IGSIC, the most enjoyable aspect was the different cultures and environments think and networking and connections established with shape their research. The sense of community the Australian students that were from Perth was further strengthened by attending the and Melbourne.

AASISI iiss nownow onon FacebookFacebook andand TwitterTwitter For up-to-date information on all things ASI, including conferences, travel scholarships, prizes, visiting speakers and general immunology news.

Follow at: https://twitter.com/ASImmunology https://www.facebook.com/ASImmunology And for even more immunology news, https://twitter.com/DayofImmunology

Accounts managed by ASI member, Gabriela Khoury

30 ASI Inc. Newsletter June 2014

9th International Congress on Autoimmunity 26-30 March 2014, Nice, France Stephen Scally Monash University, Victoria The view of Nice from le château Firstly, I would like to thank the ASI for awarding me an International Travel Grant, which allowed me to attend the 9th International Congress on Autoimmunity held in Nice, France from March 26 to 30. This was a great meeting with over 2500 participants, and an excellent chance to present my research to an international audience.

The meeting began with a brush up on my immunology, thanks to a great Basic Immunology Course run by Prof Abul Abbas. The conference held six parallel sessions running over four days ensuring that there I was lucky enough to present my PhD leaders in the ﬁ eld, have in-depth discussions was always something of interest for me to work during the parallel session “Genetics about the project and it will hopefully lead see. Some of the highlights included Professor in Autoimmunity” and received some great to a collaboration in the future. Kazuhiko Yamamoto’s talk investigating questions and feedback from the audience. antigen-specific T cells in rheumatoid In addition, Dr Nathalie Lambert invited me Overall, this was a fantastic opportunity arthritis and Professor Guy Serre’s talk, to give a talk for her and Prof Jean Roudier’s to present my research at an international where he described a pathophysiological link group at INSERM U639, Parc Scientiﬁ que de conference and I gained some valuable between the presence of anti citrullinated Luminy in Marseille the following week. This feedback and experience. I’d like to thank antibodies and IgM rheumatoid factor in was a great experience to present my work to ASI once again for their generous support. rheumatoid arthritis.

SPF MICE AND RATS CUSTOMISED BREEDING MAINTENANCE OF STRAINS IMPORT AND EXPORT CRYOPRESERVATION

PO Box 1180 Canning Vale DC, Western Australia 6970 Telephone: (08) 9332 5033 Fax: (08) 9310 2839 Email: [email protected] Web site: www.arc.wa.gov.au

31 ASI Inc. Newsletter June 2014 FIMSA “Frontiers in Immune Modulation”

FIMSA2015 aims to facilitate interactions between members of its societies and to exchange knowledge in basic and clinical immunology to advance the science of immunology in the Asia-Paciﬁc region. The congress will bring together scientists from the region for this purpose.

6th Congress of the FIMSA 30 June - 3July 2015 (Federation of Immunological Societies of Asia Oceania) Sands Expo and Convention Centre, Singapore

Keynote Speaker: Tasuku HONJO, Japan

Conﬁrmed Speakers: Gabrielle BELZ, Australia Stefan KAUFMANN, Germany Su BING, China Bernard MALISSEN, France Xuetao CAO, China Diane MATHIS, United Kingdom Shubhada CHIPLUNKAR, India James McCLUSKEY, Australia Gennaro DE LIBERO, Singapore Caetano REIS e SOUSA, United Kingdom Sidonia FAGARASAN, Japan Koyasu SHIGEO, Japan Nick GASCOIGNE, Singapore Charles D SURH, South Korea Florent GINHOUX, Singapore Zhigang TIAN, China William (Bill) HEATH, Australia Carola VINUESA, Australia

Organised by: For more information, please visit www.sgsi.org.sg or email to [email protected]

32 ASI Inc. Newsletter June 2014

Publications List Congratulations to ASI members who have published their following work in the last three months

Afshar-Sterle S, Zotos D, Bernard NJ, Scherger AK, Bird CH, Christensen ME, Mangan MS, Prakash MD, producing leukocytes promote acute kidney injury Rodling L, Alsop AE, Walker J, Masson F, Belz GT, Sedelies KA, Smyth MJ, Harper I, Waterhouse NJ, Bird via infl ammasome and Toll-like receptor activation. Corcoran LM et al. Fas ligand-mediated immune PI. The granzyme B-Serpinb9 axis controls the fate The American journal of pathology 2014; 184(5): surveillance by T cells is essential for the control of lymphocytes after lysosomal stress. Cell death and 1411. of spontaneous B cell lymphomas. Nature medicine differentiation 2014; 21(6): 876. Chan CJ, Martinet L, Gilfi llan S, Souza-Fonseca- 2014; 20(3): 283. Birkinshaw RW, Kjer-Nielsen L, Eckle SB, McCluskey Guimaraes F, Chow MT, Town L, Ritchie DS, Colonna Alaidarous M, Ve T, Casey LW, Valkov E, Ericsson DJ, J, Rossjohn J. MAITs, MR1 and vitamin B metabolites. M, Andrews DM, Smyth MJ. The receptors CD96 Ullah MO, Schembri MA, Mansell A, Sweet MJ, Kobe Current opinion in immunology 2014; 26: 7. and CD226 oppose each other in the regulation B. Mechanism of bacterial interference with TLR4 of natural killer cell functions. Nature immunology Bisht K, Wegiel B, Tampe J, Neubauer O, Wagner KH, signaling by Brucella Toll/interleukin-1 receptor 2014; 15(5): 431. Otterbein LE, Bulmer AC. Biliverdin modulates the domain-containing protein TcpB. The Journal of expression of C5aR in response to endotoxin in part Chevrier S, Corcoran LM. BTB-ZF transcription biological chemistry 2014; 289(2): 654. via mTOR signaling. Biochemical and biophysical factors, a growing family of regulators of early Altin JA, Daley SR, Howitt J, Rickards HJ, Batkin AK, research communications 2014. and late B-cell development. Immunology and cell Horikawa K, Prasad SJ, Nelms KA, Kumar S, Wu LC biology 2014. Bonder CS, Ebert LM. Fos-icking for control of et al. 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Skin Develops an Enhanced Infl ammatory Response Vincent FB, Morand EF, Schneider P, Mackay F. The system. Oncoimmunology 2014; 3(1): e27414. to 2,4-Dinitrochlorobenzene by an Arginase-1- BAFF/APRIL system in SLE pathogenesis. Nature Wolf P, Gruber-Wackernagel A, Bambach I, Schmidbauer Dependent Mechanism. The Journal of investigative reviews. Rheumatology 2014. U, Mayer G, Absenger M, Frohlich E, Byrne SN. dermatology 2014. Vogelzang A, McGuire HM, Liu SM, Gloss B, Mercado Photohardening of polymorphic light eruption Uldrich AP, Le Nours J, Pellicci DG, Gherardin NA, K, Earls P, Dinger ME, Batten M, Sprent J, King C. patients decreases baseline epidermal Langerhans McPherson KG, Lim RT, Patel O, Beddoe T, Gras S, IL-21 contributes to fatal infl ammatory disease in cell density while increasing mast cell numbers in the Rossjohn J et al. CD1d-lipid antigen recognition by the absence of Foxp3+ T regulatory cells. Journal of papillary dermis. Experimental dermatology 2014. the gammadelta TCR. Nature immunology 2013; immunology 2014; 192(4): 1404. Yang Z, Robinson MJ, Allen CD. Regulatory 14(11): 1137. von Scheidt B, Leung PS, Yong MC, Zhang Y, Towne JE, constraints in the generation and differentiation of Van Rhijn I, Gherardin NA, Kasmar A, de Jager W, Smyth MJ, Teng MW. Combined Anti-CD40 and Anti- IgE-expressing B cells. Current opinion in immunology Pellicci DG, Kostenko L, Tan LL, Bhati M, Gras S, IL-23 Monoclonal Antibody Therapy Effectively 2014; 28C: 64. Godfrey DI et al. TCR bias and affi nity defi ne two Suppresses Tumor Growth and Metastases. Cancer Zaki MH, Man SM, Vogel P, Lamkanfi M, Kanneganti compartments of the CD1b-glycolipid-specifi c T research 2014; 74(9): 2412. TD. Salmonella exploits NLRP12-dependent innate Cell repertoire. Journal of immunology 2014; 192(9): West AC, Mattarollo SR, Shortt J, Cluse LA, immune signaling to suppress host defenses during 4054. Christiansen AJ, Smyth MJ, Johnstone RW. An intact infection. Proceedings of the National Academy Vella LJ, Pasam A, Dimopoulos N, Andrews M, Knights immune system is required for the anticancer of Sciences of the United States of America 2014; A, Puaux AL, Louahed J, Chen W, Woods K, Cebon JS. activities of histone deacetylase inhibitors. Cancer 111(1): 385. MEK inhibition, alone or in combination with BRAF research 2013; 73(24): 7265. Zhang J, Yan S, Sluyter R, Li W, Alici G, Nguyen inhibition, affects multiple functions of isolated West AC, Smyth MJ, Johnstone RW. The anticancer NT. Inertial particle separation by differential normal human lymphocytes and dendritic cells. effects of HDAC inhibitors require the immune equilibrium positions in a symmetrical serpentine Cancer immunology research 2014; 2(4): 351. micro-channel. Scientifi c reports 2014; 4: 4527.