cancers Article The Constitutive Extracellular Protein Release by Acute Myeloid Leukemia Cells—A Proteomic Study of Patient Heterogeneity and Its Modulation by Mesenchymal Stromal Cells Elise Aasebø 1,2 , Annette K. Brenner 1, Even Birkeland 2, Tor Henrik Anderson Tvedt 3, Frode Selheim 2, Frode S. Berven 2 and Øystein Bruserud 2,3,* 1 Department of Clinical Science, University of Bergen, 5021 Bergen, Norway;
[email protected] (E.A.);
[email protected] (A.K.B.) 2 The Proteomics Facility of the University of Bergen (PROBE), University of Bergen, 5009 Bergen, Norway;
[email protected] (E.B.);
[email protected] (F.S.);
[email protected] (F.S.B.) 3 Department of Medicine, Haukeland University Hospital, 5021 Bergen, Norway;
[email protected] * Correspondence:
[email protected] or
[email protected] Simple Summary: The formation of normal blood cells in the bone marrow is supported by a network of non-hematopoietic cells including connective tissue cells, blood vessel cells and bone-forming cells. These cell types support and regulate the growth of acute myeloid leukemia (AML) cells Citation: Aasebø, E.; Brenner, A.K.; and communicate with leukemic cells through the release of proteins to their common extracellular Birkeland, E.; Tvedt, T.H.A.; Selheim, F.; Berven, F.S.; Bruserud, Ø. The microenvironment. One of the AML-supporting normal cell types is a subset of connective tissue Constitutive Extracellular Protein cells called mesenchymal stem cells. In the present study, we observed that AML cells release a wide Release by Acute Myeloid Leukemia range of diverse proteins into their microenvironment, but patients differ both with regard to the Cells—A Proteomic Study of Patient number and amount of released proteins.