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IMMUNE RESPONSE 2 Antibodies for Immune Response ptglab.com 1 ANTIBODIES FOR IMMUNE RESPONSE www.ptglab.com 2 Antibodies For Immune Response Front Cover: Immunohistochemistry of paraffin-embedded human oesophagus tissue slide using VEGFA antibody (19003-1-AP) at a dilution of 1:200 (10x objective). ptglab.com 3 WELCOME The human immune system contains a collection of different cell types and molecules that help to protect the body from toxins, viral infections, bacteria, and parasites. Although immunology covers all aspects of this complex network, the field is united by the immune cell. The central points of immunology are: understanding, identifying, and distinguishing the many different immune cells that are essential for diagnosis and therapy. This includes research on both the innate and adaptive immune response and processes such as pathogen recognition, immune cell activation and recruitment, cytokine secretion, inflammation, and antibody production. Cell markers are helpful tools for immunophenotyping diverse types of human immune cells. This catalog offers a detailed choice of the most commonly used cell markers and an extensive selection of antibodies used in immunology. What’s Inside 4–5 Product Focus – Natural killer (NK) cells and NCAM1/CD56 – CD20 and B cells – A keystone cytokine – the many faces of Interleukin-6 (IL-6) – Multiple roles for Tumor Necrosis Factor-Alpha (TFNA/TNFSF2) 6–17 Antibodies: AGER ZC3HAV1 18 Contact Us Please Note: All products featured in this catalog are for research use only. *Proteintech® and the Proteintech logo are trademarks of Proteintech Group registered in the US Patent and Trademark Office. 4 Antibodies For Immune Response PRODUCT FOCUS Natural killer (NK) cells Natural killer (NK) cells are the most is also expressed on the surface of human responsive immune cells to acute exercise. neurons, glial cells, and skeletal muscle cells. Their sensitivity to physiological stress It is also involved in the expansion of CD4+ and NCAM1/CD56 combined with their role in innate immune and CD8+ T cells and dendritic cells, which defenses shows that there is a link between play an important role in immune surveillance. Focus Antibody regular physical activity and overall health There are three main forms of human NCAM-1 NCAM1/CD56 status. NK cells are a heterogeneous that arise by alternative splicing: 120kDa (761 population consisting of at least two distinct amino acids (aa) (glycosylphosphatidylinositol subsets based on the expression intensity (GPI) anchored)), 140kDa (848 aa, short Catalog Number of neural cell adhesion molecule 1 (NCAM1, cytoplasmic domain), and 180kDa (1120 14255-1-AP also known as CD56). aa, long cytoplasmic domain). NCAM1/CD56 is a cell adhesion molecule NCAM1/CD56 is already broadly Type involved in neuron-neuron adhesion, neurite used as a sensitive and diagnostically fasciculation, an outgrowth of neurites, useful immunohistochemical marker for Rabbit Polyclonal synaptic plasticity, neurodevelopment, problematical small cell lung carcinomas and neurogenesis. Besides NK cells, NCAM1 or neuroendocrine tumors. Applications ELISA, FC, IF, IHC, WB 6 Publications Immunohistochemical staining of paraffin-embedded human colon slide using NCAM1 antibody (14255-1-AP) at a dilution of 1:400. CD20 and B cells CD20 is a 33-37 kDa transmembrane CD20 serves as a useful target for antibody- phosphoprotein. B-lymphocyte antigen mediated therapeutic depletion of B cells. CD20 is also known as B-lymphocyte surface It is the target of the monoclonal antibodies Focus Antibody antigen B1, Leukocyte surface antigen Leu-16, (mAb) rituximab, Ibritumomab tiuxetan, and CD20 Membrane-spanning 4-domains subfamily tositumomab, which are all active agents in A member 1, and MS4A1. It is an activated- the treatment of all B-cell lymphomas Catalog Number glycosylated protein expressed on the and leukemias. surface of all B cells beginning at the pro-B 24828-1-AP phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. Type CD20 is expressed in all stages of B cell development except the first and last. Rabbit Polyclonal Applications ELISA, FC, IHC, IP, WB 1X10^6 Raji cells were stained with 0.2 ug Immunofluorescent staining of 4% PFA fixed MS4A1/CD20 antibody (24828-1-AP, red) human tonsillitis tissue using CD20 antibody and control antibody (blue). Fixed with 4% (24828-1-AP) at a dilution of 1:50 and Alexa PFA blocked with 3% BSA (30 min). Alexa Fluor Fluor 488-conjugated AffiniPure Goat Anti- 488-Goat anti-Rabbit IgG at a dilution of 1:100. Rabbit IgG (H+L) (10 x objective). ptglab.com 5 A keystone cytokine – Interleukin-6 (IL-6) acts as both a pro- IL-6 is a molecule with multiple forms inflammatory and an anti-inflammatory and functions depending on where it is cytokine. It is an endogenous chemical, secreted. IL-6 helps T cells to differentiate the many faces of active in inflammation and in B cell maturation. in their development. It is required for Interleukin-6 (IL-6) In the early phase of infectious progenitor cell development and helps B cells inflammation, IL-6 is produced by differentiate and proliferate, and it promotes monocytes and macrophages immediately the formation of plasma cells from B cells. Focus Antibody after the stimulation of Toll-like receptors IL-6 also plays a vital role in the development IL-6 (TLRs) with distinct pathogen-associated of blood cells, whether white cells, red cells, molecular patterns (PAMPs). In non-infectious or platelets. IL-6 also leads to the activation Catalog Number inflammations, such as a burn or traumatic of osteoclasts and osteoporosis. It also injury, damage-associated molecular patterns induces the secretion of vascular endothelium 21865-1-AP (DAMPs) from damaged or dying cells growth factor (VEGF), which leads to the stimulate TLRs to produce IL-6. This acute increased growth of blood vessels and Type IL-6 expression plays a significant role in vascular permeability in inflammation. host defense by stimulating various cell Genetic variations of IL6 are associated with Rabbit Polyclonal populations. IL-6 is released by monocytes rheumatoid arthritis systemic juvenile (RASJ), and macrophages in response to other and polymorphism of IL6 promoter can be an Applications inflammatory cytokines, which include indicator for Kaposi sarcoma development in interleukin-11 and tumor necrosis HIV-infected men. ELISA, IHC, WB factor (TNF)-beta. 28 Publications KD/KO Validated Immunohistochemical staining of paraffin-embedded human tonsillitis slide using IL6 antibody (21865-1-AP) at a dilution of 1:50. Multiple roles for Tumor Tumor necrosis factor-alpha (TNFA/TNFSF2) leads to the expression of a large number is an important multifunctional cytokine of genes. TNF plays a significant role in that regulates inflammation, host defense, innate immunity and host defense against Necrosis Factor-Alpha immune responses, and apoptosis. TNFA/ bacterial, fungal, and parasitic pathogens TNFSF2 signals through two distinct cell and is particularly important for host defense (TNFA/TNFSF2) surface receptors, TNFR1 (TNFRSF1A, against intracellular organisms such as CD120a, p55) and TNFR2 (TNFRSF1B, Mycobacterium tuberculosis. Dysregulation Focus Antibody CD120b, p75). TNFR1 is widely expressed, of TNFA/TNFSF2 has been found to be involved TNFR2 whereas TNFR2 exhibits more restricted in tumorigenesis, tumor metastasis, viral expression, being found on CD4 and CD8 replication, septic shock, fever, inflammation, T lymphocytes, endothelial cells, microglia, autoimmune diseases including Crohn’s Catalog Number oligodendrocytes, neuron subtypes, disease, in addition to rheumatoid arthritis 19272-1-AP cardiac myocytes, thymocytes, and human and graft-versus-host disease. mesenchymal stem cells. TNFA/TNFSF2 Recently, growing evidence has shown that Type is mainly secreted by macrophages. a blocking of TNFA/TNFSF2 action in patients The main biologic function is to induce and experimental animal models increases Rabbit Polyclonal inflammation via the upregulation of gene disease activity and severity in some T-cell- transcription, primarily through the NF- dependent autoimmune diseases (e.g., Applications kB and AP-1 signaling pathways, which multiple sclerosis or Type 1 diabetes). ELISA, FC, IF, IHC, IP, WB 4 Publications KD/KO Validated 1X10^6 HeLa cells were stained with 0.5 ug IP result of anti-TNFR2 (IP: 19272-1-AP, 4 ug; TNFR2 antibody (19272-1-AP, red) and control Detection: 19272-1-AP 1:500) with HEK-293 antibody (blue). Fixed with 4% PFA blocked cells lysate 1200 ug. with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100. 6 Antibodies For Immune Response AGER Caspase 8 ANTIBODY PRODUCT LIST Antibody Name Cat. No. Type Applications Antibody Name Cat. No. Type Applications AGER 1 16346-1-AP Rabbit Poly ELISA, IHC, WB C3/C3b/C3c 66157-1-Ig Mouse Mono ELISA, IF, IHC, WB ALCAM 21972-1-AP Rabbit Poly ELISA, IHC, WB C3/C3b/C3c 1 21337-1-AP Rabbit Poly ELISA, IHC, IP, WB C4 AMIGO2 14076-1-AP Rabbit Poly ELISA, WB 22233-1-AP Rabbit Poly ELISA, IHC, IP, WB (alpha chain) AMIGO3 21278-1-AP Rabbit Poly ELISA, IF, WB C4 66242-1-Ig Mouse Mono ELISA, WB (gamma chain) AP1, JUN, P39 11 10024-2-AP Rabbit Poly ELISA, IF, IHC, IP, WB C4BPA 1 11819-1-AP Rabbit Poly ELISA, IHC, WB AP1, JUN, P39 22114-1-AP Rabbit Poly ELISA, IF, WB C4BPB 15837-1-AP Rabbit Poly ELISA, IHC, WB ASGR1 11739-1-AP Rabbit Poly ELISA, FC, IHC, IP, WB ELISA, FC, IF, IHC, IP, C5aR 2 21316-1-AP Rabbit Poly WB ASGR2 11501-2-AP Rabbit Poly ELISA, FC, IP, WB C6 17239-1-AP Rabbit Poly ELISA, IHC, IP, WB ATG12 3 11122-1-AP
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