Quick viewing(Text Mode)

Supplementary Materials

Supplementary Materials

Supplementary Materials

Supplementary Material

1 List of Resources used in the Identification and Assigning of Trophic Role of Invertebrate Specimens

1.1 Peer Reviewed Articles Barber, B. J., J. S. Fajans, S. M. Baker, and P. Baker. 2005. Gametogenesis in the non-native green mussel, Perna viridis, and the native scorched mussel, exustus, in Tampa Bay, Florida. Journal of Shellfish Research 24:1087-1095. Bergquist, D. C., J. A. Hale, P. Baker, and S. M. Baker. 2006. Development of ecosystem indicators for the Suwannee River : oyster reef habitat quality along a salinity gradient. and Coasts 29:353-360. Boudreaux, M. L., J. L. Stiner, and L. J. Walters. 2006. of sessile and motile macrofauna on intertidal oyster reefs in Mosquito , Florida. Journal of Shellfish Research 25:1079-1089. Bueno, M., S. A. Dena-Silva, A. A. V. Flores, and F. P. P. Leite. 2016. Effects of wave exposure on the abundance and composition of amphipod and Tanaidacean assemblages inhabiting intertidal coralline . Journal of the Marine Biological Association of the United Kingdom FirstView:1-7. Coleman, C. O. 2011. Batea aldebaranae sp nov (Crustacea: Amphipoda: Bateidae) from the coast of Belize with redescriptions of two related . Journal of the Marine Biological Association of the United Kingdom 91:455-469. Collin, R. 2002. Another last word on convexa with a description of C. ustulatulina n. sp ( : ) from the Gulf of Mexico and southern Florida. Bulletin of Marine Science 70:177-184. Collin, R. 2000. Phylogeny of the (Gastropoda : Calyptraeidae) cryptic species complex in . Canadian Journal of Zoology-Revue Canadienne De Zoologie 78:1500- 1514. Collin, R. 2001. The effects of mode of development on phylogeography and population structure of North Atlantic Crepidula (Gastropoda: Calyptraeidae). Molecular ecology 10:2249-2262. Cote, J., C. F. Rakocinski, and T. A. Randall. 2001. Feeding efficiency by juvenile blue crabs on two common species of micrograzer snails. Journal of Experimental Marine Biology and Ecology 264:189-208. Defreese, D. E. 1987. Ecology and Burrowing Behavior of ulla (, Ascoglossa). 30:40-45. deMaintenon, M. J., and P. M. Mikkelsen. 2001. Late reproductive system development in two Cephalaspideans (Gastropoda : Opisthobranchia): Bulla striata Bruguiere, 1792, and atrata Mikkelsen & Mikkelsen, 1984. Veliger 44:237-260.

1 Supplementary Materials

Douglass, J. G., J. E. Duffy, and E. A. Canuel. 2011. structure in a Chesapeake Bay eelgrass bed as determined through gut contents and 13C and 15N isotope analysis. Estuaries and Coasts 34:701-711. Drumm, D. T. 2005. Comparison of feeding mechanisms, respiration, and cleaning behavior in two Kalliapseudids, Kalliapseudes macsweenyi and Psammokalliapseudes granulosus ( : ). Journal of Biology 25:203-211. Drumm, D. T., and B. Kreiser. 2012. Population genetic structure and phylogeography of Mesokalliapseudes macsweenyi (Crustacea: Tanaidacea) in the northwestern Atlantic and Gulf of Mexico. Journal of Experimental Marine Biology and Ecology 412:58-65. Ewald, J. J. 1969. Observations on biology of Tozeuma carolinense (, Hippolytidae) from Florida, with special reference to larval development. Bulletin of Marine Science 19:510-549. Franz, D. R., and J. T. Tanacredi. 1992. Secondary production of the amphipod Ampelisca abdita mills and its importance in the diet of juvenile winter flounder (Pleuronectes americanus) in Jamaica Bay, New-York. Estuaries 15:193-203. Frick, M. G., K. L. Williams, D. Veljacic, L. Pierrard, J. A. Jackson, and S. E. Knight. 2000. Newly documented epibiont species from nesting loggerhead sea turtles (Caretta caretta) in Georgia, USA. Marine Turtle Newsletter 88:3-5. Greening, H. S., and R. J. Livingston. 1982. Diel variation in the structure of seagrass-associated epibenthic macroinvertebrate communities. Marine Ecology Progress Series 7:147-156. Grizzle, R. E., J. R. Adams, and L. J. Walters. 2002. Historical changes in intertidal oyster (Crassostrea virginica) reefs in a Florida lagoon potentially related to boating activities. Journal of Shellfish Research 21:749-756. Hays, C. 2005. Effect of nutrient availability, grazer assemblage and seagrass source population on the interaction between Thalassia testudinum (turtle grass) and its algal epiphytes. Journal of Experimental Marine Biology and Ecology 314:53-68. Hoagland, K. E. 1977. Systematic review of fossil and recent Crepidula and discussion of evolution of Calyptraeidae. Malacologia 16:353-420. Houbrick, R. S. 1993. Phylogenetic relationships and generic review of the Bittiinae (, ). Malacologia 35:261-313. Houbrick, R. S. 1980. Observations on the and life history of Modulus modulus (Prosobranchia, Modulidae). Malacologia 20:117-142. Jensen, K. R. 1994. Behavioral adaptations and diet specificity of Sacoglossan Opisthobranchs. Ethology Ecology & Evolution 6:87-101. Lefcheck, J. S., S. R. Marion, and R. J. Orth. in press. Actively restored ecosystems as a refuge for biological diversity: a case study from eelgrass (Zostera marina L.). PeerJ.

2 Supplementary Materials

Lefcheck, J. S., and J. E. Duffy. 2015. Multitrophic functional diversity predicts ecosystem functioning in experimental assemblages of estuarine consumers. Ecology 96:2973-2983.

Magalhaes, H. 1948. An ecological study of snails of the Busycon at Beaufort, North Carolina. Ecological Monographs 18:377-409. Mangum, C. P., S. L. Santos, and W. R. Rhodes. 1968. Distribution and feeding in onuphid cuprea (Bosc). Marine Biology 2:33-40. Manning, R. B., and D. L. Felder. 1989. The Pinnixa cristata complex in the western Atlantic with a description of two new species (Crustacea: Decapoda: Pinnotheridae). Smithsonian Contributions to Zoology 473:1-26. Marin, A., L. A. Alvarez, G. Cimino, and A. Spinella. 1999. Chemical defence in Cephalaspidean gastropods: Origin, anatomical location and ecological roles. Journal of Molluscan Studies 65:121- 131. Marsh, G. A. 1976. Ecology of the gastropod epifauna of eelgrass in a Virginia, USA Estuary. Chesapeake Science 17:182-187. Martin, T. H., R. A. Wright, and L. B. Crowder. 1989. Non-additive impact of blue crabs and spot on their prey assemblages. Ecology 70:1935-1942. Malaquias, M. A. E., E. Berecibar, and D. G. Reid. 2009. Reassessment of the trophic position of Bullidae (Gastropoda: ) and the importance of diet in the evolution of cephalaspidean gastropods. Journal of Zoology 277:88-97. McMahon, R. F., and W. D. Russellhunter. 1977. Temperature relations of aerial and aquatic respiration in 6 littoral snails in relation to their vertical zonation. Biological Bulletin 152:182-198. Miller, A. W., G. M. Ruiz, M. S. Minton, and R. F. Ambrose. 2007. Differentiating successful and failed molluscan invaders in estuarine ecosystems. Marine Ecology Progress Series 332:41-51. Moore, H. B., and N. N. Lopez. 1972. Contribution to ecology of Lamellibranch alba. Bulletin of Marine Science 22:381-390. Murray, J. B., and G. L. Wingard. 2006. Salinity and temperature tolerance experiments on selected Florida Bay mollusks. US Geological Survey Open-File Report 2006-1026:1-17. Newell, R. I. E., and S. J. Jordan. 1983. Preferential ingestion of organic material by the American oyster Crassostrea virginica. Marine Ecology Progress Series 13:47-53. -Rivera, J. J. 2003. The amphipod of Banco Chinchorro, Quintana Roo, Mexico with ecological notes. Bulletin of Marine Science 73:77-89. Osman, R. W., and R. B. Whitlatch. 1995. on early ontogenic life stages and its effect on recruitment into a marine epifaunal community. Marine Ecology Progress Series 117:111-126. Osman, R. W., R. B. Whitlatch, and R. J. Malatesta. 1992. Potential role of micropredators in determining recruitment into a marine community. Marine Ecology Progress Series 83:35-43.

3 Supplementary Materials

Paz-Ríos, C. E., N. Simões, and P. Ardisson. 2013. Intertidal and shallow water amphipods (Amphipoda: Gammaridea and Corophiidea) from Isla Pérez, Alacranes Reef, southern Gulf of Mexico. Nauplius 21:179-194. Peiro, D. F., and F. L. Mantelatto. 2011. Population dynamics of the pea crab Austinixa aidae (Brachyura, Pinnotheridae): a symbiotic of the ghost (Thalassinidea, ) from the southwestern Atlantic. Iheringia Serie Zoologia 101:5-14. Posey, M. H. 1986. Changes in a benthic community associated with dense beds of a burrowing deposit feeder, Callianassa californiensis. Marine Ecology Progress Series 31:15-22. Prager, E. J., and R. B. Halley. 1999. The influence of seagrass on shell layers and Florida Bay mudbanks. Journal of Coastal Research 15:1151-1162. Radabaugh, K. 2013. Light-environment controls and basal resource use of planktonic and benthic primary production. PhD dissertation. University of South Florida, Saint Petersburg, Florida, USA.

Ray, B. R., M. W. Johnson, K. Cammarata, and D. L. Smee. 2014. Changes in seagrass species composition in northwestern Gulf of Mexico estuaries: effects on associated seagrass fauna. Plos One 9:e107751.

Reid, D. G., and M. Claremont. 2014. The genus Cerithideopsis Thiele, 1929 (Gastropoda: Potamididae) in the Indo-West Pacific region. Zootaxa 3779:61-80.

Riisgard, H. U. 1988. Efficiency of Particle Retention and Filtration-Rate in 6 Species of Northeast American Bivalves. Marine Ecology Progress Series 45:217-223.

Rudershausen, P. J., J. V. Locascio, and L. M. Rojas. 2003. A survey of epifauna among macrophytes in a southwest Florida estuary. Florida Scientist 66:168-183

Stoner, A. W., and H. S. Greening. 1984. Geographic variation in the macrofaunal associates of pelagic Sargassum and some biogeographic implications. Marine Ecology Progress Series 20:185- 192.

Taylor, J. D., N. J. Morris, C. N. Taylor. 1980. Food specialization and the evolution of predatory prosobranch gastropods. Palaentology 23:375–409 Tunberg, B. G., W. G. Nelson, and G. Smith. 1994. Population ecology of Pagurus maclaughlinae Garcia-Gomez (Decapoda, Anomura, Paguridae) in the Indian River Lagoon, Florida. Journal of Crustacean Biology 14:686-699. Vann, C. 1980. The ecology of the Caridean dominated shrimp community in seagrass beds off Cedar Key, Florida. PhD Dissertation. University of Florida, Gainesville, Florida, USA. van Montfrans, J., R. J. Orth, and S. A. Vay. 1982. Preliminary studies of grazing by varium on eelgrass periphyton. Aquatic Botany 14:75-89. Walker, S. E., K. Parsons-Hubbard, E. Powell, and C. E. Brett. 2002. Predation on experimentally deployed molluscan shells from shelf to slope depths in a tropical carbonate environment. Palaios 17:147-170.

4 Supplementary Materials

Weisbord, N. E. 1962. Late Cenozoic gastropods from northern Venezuela. Bulletins of American Paleontology 42:1-672. Welch, J. J. 2010. The "island rule" and deep-sea gastropods: re-examining the evidence. Plos One 5:e8776. Wise, J. B. 2000. Anatomy and systematic placement of Sayella laevigata (Gastropoda : Pyramidellidae : Sayellinae). Journal of Molluscan Studies 66:119-124. Zimmerman, R., R. Gibson, and J. Harrington. 1979. Herbivory and detritivory among Gammaridean amphipods from a Florida seagrass community. Marine Biology 54:41-47. Zupo, V., and W. G. Nelson. 1999. Factors influencing the association patterns of Hippolyte zostericola and intermedius (Decapoda : Natantia) with seagrasses of the Indian River Lagoon, Florida. Marine Biology 134:181-190.

1.2 Books, Book Chapters, and Taxonomic Keys Abbot, R. T., and P. A. Morris. 1995. Shells of the Atlantic and Gulf coasts and the West Indies. Peterson Field Guides. Houghton Mifflin Company, New York, New York, USA. Abbot, R. T., and P. A. Morris. 2001. Shells of the Atlantic and Gulf Coasts and the West Indies. Peterson Field Guides. Houghton Mifflin Company, New York, New York, USA. Andrews, J. 1977. Shells and shores of Texas. University of Texas Press, Austin, Texas, USA. Andrews, J. 1994. A field guide to shells of the Florida coast. Gulf Publishing. Houston, Texas, USA. Bellan-Santini, D., and M. J. Costello. 2001. Amphipoda. Pages 295-308 in M. J. Costello, C. Emblow, and R. J. White, editors. European register of marine species: a check-list of the marine species in and a bibliography of guides to their identification. Collection Patrimoines Naturels 50 Britton, J. C., and B. Morton. 2014. Shore ecology of the Gulf of Mexico. University of Texas Press, Austin, Texas, USA. Burbanck, W. D., and M. P. Burbanck. 1979. Cyathura (Arthropoda: Crustacea: : Anthuridae). Pages 293-323 in C. W. Hart and L. H. Fuller, editors. Pollution ecology of estuarine invertebrates. Academic Press, New York, New York, USA. Donovan, E. 1804. The natural history of British shells, including figures and descriptions of all the species hitherto discovered in Great Britain, systemically arranged in the Linnean manner, with scientific and general observations on each. E. Donovan, F. C. Rivington, and J. Rivington, London, UK. Felder, D. L., and D. K. Camp. 2009. Gulf of Mexico origin, waters, and biota. Volume 1: Biodiversity. Texas A&M University Press, College Station, Texas, USA. Gmelin, J. F. 1791. Systema naturae per regna tria naturae. Systema naturae, 13th editioin. Impensis Georg Emanuel Beer, Leipzig, Germany.

5 Supplementary Materials

Harasewych, M. G., and F. Moretzsohn. 2014. The book of shells: a life-size guide to identifying and classifying six hundred seashells. University of Chicago Press, Chicago, Illinois, USA. Heard, R. W., T. Hansknecht, and K. Larsen. 2003. An illustrated identification guide to Florida Tanaidacea (Crustacea: Peracarida) occurring in depths of less than 200 m. Annual Report. Department of Environmental Protection, Tallahassee, Florida, USA. Hendler, G. 1982. The Feeding Biology of brevispinum Ophiuroidea Echinodermata. Pages 21-27 in J. M. Lawrence, J. M., editor. : proceedings of the international conference, Tampa Bay, September 1981. CRC Press, Boca Raton, Florida, USA. Hoagland, K.E. and W.F. Ponder. 1998. Superfamily Calyptraeoidea. Pages 772-774 in P. L. Beesley, G. J. B. Ross, and A. Wells, editors. : the southern synthesis. Part B. Fauna of Australia Vol. 5. CSIRO Publishing, Melbourne, Australia. Jangoux, M., and J. M. Lawrence. 1982. nutrition. CRC Press, Boca Raton, Florida, USA. Kaplan EH. 1988. A field guide to southeastern and Caribbean seashores: Cape Hattaras to the Gulf Coast, Florida, and the Caribbean. Houghton Mifflin Company, New York, New York, USA. Lawrence, J. M. 1982. Echinoderms: proceedings of the international conference, Tampa Bay, September 1981. CRC Press, Boca Raton, Florida, USA. LeCroy. S. E. 2000. An illustrated identification guide to the nearshore marine and estuarine Gammaridean amphipoda of Florida. Volumes 1-5. Annual Report. Florida Department of Environmental Protection, Tallahassee, Florida, USA. Lippson, R. L., and A. J. Lippson. 2012. Life along the inner coast - a naturalist's guide to the sounds, inlets, rivers, and intracoastal waterways from Norfolk to Key West. Southern Gateways Guides. University of North Carolina Press, Chapel Hill, North Carolina, USA. Livingston, R. J. 2002. Trophic organization in coastal systems. CRC Press, Boca Raton, Florida, USA. Mikkelsen, P. M., and R. Bieler. 2008. Seashells of southern Florida. Princeton University Press, Princeton, New Jersey, USA. Petuch, E.J. and R.F. Myers. Molluscan communities of the Florida Keys and adjacent areas: their ecology and biodiversity. CRC Press, Boca Raton, Florida, USA. Rehder, H.A. 1981. The Audobon society field guide to North American seashells. Chanticleer Press, New York, New York, USA. Reid, R. G. B, and S.M. Slack-Smith. 1998. Superfamily Lucinoidea. Pages 309-315 in P. L. Beesley, G. J. B. Ross, and A. Wells, editors. Mollusca: the southern synthesis. Part A. Fauna of Australia Vol. 5. CSIRO Publishing, Melbourne, Australia. Richardson, H. 1905. A monograph on the isopods of North America. Smithsonian Institution, Washington, DC, USA.

6 Supplementary Materials

Rothschild, S. B. 2004. Beachcomber's guide to Gulf coast marine life: Texas, Louisiana, Mississippi, Alabama, and Florida. Taylor Trade Publications, Lanham, Maryland, USA. Ruppert, E. E., and R. S. Fox. 1988. Seashore of the southeast: a guide to common shallow- water invertebrates of the southeastern Atlantic coast. University of South Carolina Press, Columbia, South Carolina, USA. Thiel, M., and L. Watling. 2015. The natural history of Crustacea: lifestyles and feeding biology. Oxford University Press, Oxford, UK. Tunnell, J. W., Jr., J. Andrews, N. C. Barrera, and F. Moretzsohn. 2010. The encyclopedia of Texas seashells. Texans A&M University Press, College Station, Texas, USA. Vermeij, G. J. 1980. Biogeography and adaptation: patterns of marine life. Harvard University Press, Cambridge, Massachusetts, USA.

1.3 Internet Databases 1.3.1 Smithsonian Bocas del Toro Species Database. varium species profile. Smithsonian Tropical Research Institute. Page undated. Accessed Jan 2, 2016. Chesapeake Bay Database. Monocorophium acherusicum. Smithsonian Environmental Research Center. Page last updated Sept 21, 2005. Accessed Jan 2, 2016. Collin, R. Crepidula atrasol. Smithsonian Marine Station. Page last updated Jan 2, 2005. Accessed Oct 16, 2014. Collin, R. Crepidula depressa. Smithsonian Marine Station. Page last updated Jan, 2 2005. Accessed Oct 16, 2014. Collin, R. Crepidula ustulatulina. Smithsonian Marine Station. Page last updated Jan 2, 2005. Accessed Oct 25, 2014. Field guide to the Indian River Lagoon. Palaemon floridanus. Smithsonian Marine Station. Page undated. Accessed Jan 2, 2016. Hill, K. Penaeus duorarum. Smithsonian Marine Station. Page last updated July 9, 2002. Accessed Jan 2, 2016. Masterson, J. Alpheus heterochaelis. Smithsonian Marine Station. Page last updated Sept 1, 2008. Accessed Jan 2, 2016. Masterson, J. . Smithsonian Marine Station. Page last updated Oct 1, 2008. Accessed Jan 2, 2016. Masterson, J. Bulla striata. Smithsonian Marine Station. Page last updated Oct 1 2008. Accessed Oct 25 2014.

7 Supplementary Materials

Masterson, J. Cymadusa compta. Smithsonian Marine Station. Page last updated Oct 1, 2008. Accessed Jan 2, 2016. Masterson, J. Hargeria rapax. Smithsonian Marine Station. Page last updated Oct 1, 2008. Accessed Jan 2, 2016. Masterson, J. Modulus modulus. Smithsonian Marine Station. Page last updated Oct, 1 2008. Accessed Oct 25, 2014. Puglisi, M. P. Brachidontes exustus. Smithsonian Marine Station. Page last updated Oct 1, 2008. Accessed Jan 2, 2016. Puglisi, M. P. Diodora cayensis. Smithsonian Marine Station. Page last updated Oct, 1 2008. Accessed Sept 23, 2015. Puglisi, M. P. Dyspanopeus sayi. Smithsonian Marine Station. Page last updated Oct 1, 2008. Accessed Jan 2, 2016. Puglisi, MP. lunata. Smithsonian Marine Station. Page last updated Oct 1 2008. Accessed Oct 25 2014. Sweat, L. H. Anodontia alba. Smithsonian Marine Station. Page last updated Sept 28, 2010. Accessed Oct 13, 2014. Sweat, L. H. apicinum. Smithsonian Marine Station. Page last updated Sept, 28 2010. Accessed Sept 23, 2015 1.3.2 Fossilworks Fossilworks. Agathotoma Cossmann 1899 (). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 30, 2015. Fossilworks. Anachis (Costoanachis) Sacco 1890 (snail). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 30, 2015. Fossilworks. Arcopsis adamsi Dall 1886 (clam). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 24 2015. Fossilworks. Astyris lunata Say 1826 (snail). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 30, 2015. Fossilworks. Carditamera floridana Conrad 1838 (clam). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 30, 2015. Fossilworks. Columbella rusticoides Heilprin 1887 (snail). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 30, 2015.

8 Supplementary Materials

Fossilworks. Conus mindanus Hwass 1792 (cone shell). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 30, 2015. Fossilworks. Crepidula Lamarck 1799 (slipper shell). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 30, 2015. Fossilworks. Cyclostremiscus (Cyclostremiscus) Pilsbry and Olsson 1945 (snail). Fossilworks: Gateway to the Paleobiology Database Page undated. Accessed Sept 30, 2015. Fossilworks. Family Costellariidae MacDonald 1860 (ribbed mitre). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 30, 2015. Fossilworks. Family Rafinesque 1815 (murex snail). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 30 2015. Fossilworks. Family Turridae Swainson 1840 (turrid). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 30, 2015. Fossilworks. Modulus modulus Linnaeus 1758 (snail). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 30, 2015. Fossilworks. Duméril 1806 (snail). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 30, 2015. Fossilworks. Swainson 1831 (). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 30, 2015. Fossilworks. Bartsch 1950 (turrid). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 30, 2015. Fossilworks. Menke 1828 (margin shell). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 30, 2015. Fossilworks. plicosa Adams 1850 (turrid). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 24 2015. Fossilworks. Rissoina (Phosinella) Mörch 1876 (snail). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 30, 2015.

9 Supplementary Materials

Fossilworks. Rissoina () Nevill 1881 (snail). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 30, 2015. Fossilworks. Rubellatoma Bartsch and Rehder 1939 (turrid). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 30, 2015. Fossilworks. Terebra protexta Conrad 1845 (auger snail). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 30, 2015. Fossilworks. Hinds 1844 (margin shell). Fossilworks: Gateway to the Paleobiology Database. Page undated. Accessed Sept 30 2015. 1.3.3 Jaxshells Krusberg, M. F. The Tampa Drill - Urosalpinx tampaensis (Conrad, 1846). Adapted from an article published in The Capsule, Vol. 30, 1998. Accessed Sept 30, 2015. 1.3.4 Gastropods.com Nassarius (Nassarius) consensus. Last updated Sept 21, 2015. Accessed Oct 1, 2015. 1.3.5 Encyclopedia of Life Encyclopedia of Life. Costoanachis semiplicata. Page undated. Accessed Oct 1, 2015. 1.3.6 World Register of Marine Species WoRMS Editorial Board. 2016. World Register of Marine Species. Accessed Jan 2, 2016. at VLIZ. 1.3.7 Let’s Talk Seashells Let’s Talk Seashells. Discussions/presentation at the species level – univalves. Page undated. Accessed Jan 2, 2016. Let’s Talk Seashells. Discussions/presentation at the species level – bivalves. Page undated. Accessed Jan 2, 2016. 1.3.8 Malacolog Rosenberg, G. 2009. Malacolog 4.1.1: A database of western Atlantic marine mollusca. [WWW database (version 4.1.1)] 1.3.9 Bailey-Mathews National Shell Museum Leal, J.H. Southwest Florida Shells with emphasis on Sanibel & Captiva: Family Muricidae. Bailey- Mathews National Shell Museum. Page undated. Accessed Sept 30 2015.

10 Supplementary Materials

1.3.10 Molluscan Life Habits Databases Todd, J. A. Introduction to molluscan life habits databases. Page updated on March 27, 2001. Accessed Jan 19, 2016.

11 Supplementary Materials

2 Table S1. Summary of macroinvertebrate specimens collected from estuarine systems along a latitudinal gradient in August 2014.

Total System Phylum: Class: Order: Genus: Species Abundance Weeki Wachee Annelida Polychaeta -- -- Polychaete spp. 114 Amphinomida Amphinomidae Amphinomidae sp. 3 Eunicidae

Eunicidae sp. A 157 Eunicidae sp. B 148 Lumbrineridae Lumbrineridae sp. A 27 Diopatra sp. 2 Phyllodocida Polynoidae Polynoidae sp. A 1 Arthropoda Amphipoda -- Gammaridea spp. 37 Ampeliscidae Ampelisca abdita 51 Ampithoidae Ampithoe longimana 19 Cymadusa compta 207 Aoridae Globosolembos smithi 2 Grandidierella bonnieroides 209 Bateidae Batea cuspidata 11

12 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Weeki Wachee Corophiidae Apocorophium simile 1 Monocorophium acherusicum 8 Gammaridae Gammarus mucronatus 1 Ischyroceridae Ericthonius punctatus 2 Lysianassidae Lysianopsis alba 513 Maeridae Elasmopus levis 79 Melitidae Melita nitida 1 Decapoda Alpheidae Alpheus heterochaelis 49 Alpheus packardii 26 Alpheus sp. 3 Epialtidae Epialtus dilatatus 7 Libinia dubia 7 Pelia mutica 1 Pitho spp. 13 Hippolytidae Hippolyte pleuracantha 154 Hippolyte sp. 13 Hippolyte zostericola 68 Thor dobkini 57 Tozeuma carolinense 17 Inachidae Metoporhaphis calcarata 1 Paguridae Pagurus maclaughlinae 1280 Palaemon floridanus 4 Palaemonetes pugio 3 Panopeidae Dyspanopeus sayi 1 Neopanope packardii 181 Penaeidae Penaeus duorarum 5 Pinnotheridae Pinnixa cylindrica 27 Portunidae

13 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Weeki Wachee Callinectes sapidus 1 Isopoda -- Idotea sp. A 5 Idotea sp. B 4 Anthuridae Cyathura polita 15 Idoteidae Edotia triloba 4 Erichsonella attenuata 52 Erichsonella filiformis/floridana 9 Harrieta faxoni 57 Paracerceis caudata 18 Tanaidacea Kalliapseudidae Mesokalliapseudes macsweenyi 4 Leptocheliidae Leptochelia forresti 1 Leptochelia rapax 3 Echinodermata Asteroidea Echinaster serpentarius 1 Ophiuroidea Amphioplus abditus 72 Ophioderma brevispina 13 Mollusca Lyonsiidae Lyonsia floridana 18 Lucinoida Lucinidae Anodontia alba 1 Codakia orbicularis 31 Mytiloida Brachidontes exustus 176 Veneroida

14 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Weeki Wachee Cardiidae Laevicardium mortoni 86 Montacutidae Mysella planulata 6 Semelidae Cumingia lamellosa 1 Tellinidae Ameritella versicolor 4 Psammotreta intastriata 1 Tellina texana 2 Veneridae Chione elevata 4 Transennella conradina 45 Transennella stimpsoni 12 Gastropoda -- Pyramidellidae Sayella laevigata 9 Turbonilla sp. 3 Bittiolum varium 36 atratum 7 Cerithium muscarum 173 Cerithiopsidae Cerithiopsis sp. 8 Modulidae Modulus modulus 197 Cephalaspidea Acteocinidae Acteocina candei 8 Bullidae Bulla occidentalis 393 imbricatum 166 585 nitidum 19 Calyptraeidae Crepidula plana complex 1 Crepidula spp. 275 Vitreolina conica 5

15 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Weeki Wachee Phosinella cancellata complex 1 2920 Cyclostremiscus suppressus 1 Vitrinella floridana 17 Busyconidae Fulguropsis spirata 1 Astyris lunata 410 Columbella rusticoides 3 Costoanachis semiplicata 61 Conasprella spp. 1 Drilliidae Cerodrillia spp. 4 Cinctura hunteria 2 Pyrgocythara filosa 10 Pyrgocythara hemphilli 16 Rubellatoma diomedea 1 eburneolus 30 Granulina hadria 3 Prunum apicinum 198 Muricidae Urosalpinx tampaensis 2 Nassarius albus/concensus 60 Nassarius vibex 109 Olivellidae Olivella spp. 33 113 Terebridae Terebra protexta 6 Volvatellidae Ascobulla ulla 7 Polyplacophora Ischnochitonidae papillosus 74

16 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Weeki Wachee Sipuncula Sipunculidae Golfingiida Phascolionidae Phascolion sp. 90

17 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Chassahowitzka Annelida Polychaeta -- -- Polychaete spp. 38 Amphinomida Amphinomidae Amphinomidae sp. 1 Eunicida Eunicidae Eunicidae sp. A 94 Eunicidae sp. B 112 Lumbrineridae Lumbrineridae sp. A 62 Onuphidae Diopatra sp. 1 Arthropoda Malacostraca Amphipoda -- Gammaridea spp. 42 Ampeliscidae Ampelisca abdita 81 Ampithoidae Ampithoe longimana 9 Cymadusa compta 242 Aoridae Bemlos spinicarpus 2 Grandidierella bonnieroides 25 Bateidae Batea cuspidata 129 Corophiidae Apocorophium simile 5 Laticorophium baconi 1 Monocorophium acherusicum 7 Gammaridae Gammarus mucronatus 32 Ischyroceridae Ericthonius punctatus 2 Liljeborgiidae Idunella cf barnardi 3 Lysianassidae Lysianopsis alba 325 Maeridae

18 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Chassahowitzka Elasmopus levis 56 Melitidae Dulichiella appendiculata 1 Phoxocephalidae Phoxocephalidae sp. 7 Platyischnopidae Eudevenopus honduranus 3 Decapoda Alpheidae Alpheus heterochaelis 151 Alpheus sp. 5 Epialtidae Libinia dubia 6 Pitho spp. 5 Hippolytidae Hippolyte pleuracantha 239 Hippolyte sp. 43 Hippolyte zostericola 146 Thor dobkini 60 Tozeuma carolinense 12 Paguridae Pagurus maclaughlinae 718 Palaemonidae Palaemon floridanus 46 Palaemonetes intermedius 2 Panopeidae Neopanope packardii 63 Penaeidae Penaeus duorarum 7 Pinnotheridae Pinnixa cylindrica 22 Portunidae Callinectes sapidus 3 Isopoda -- Idotea sp. A 1 Anthuridae Cyathura polita 18 Arcturidae Arcturidae sp. A 5 Idoteidae Edotia triloba 1 Erichsonella attenuata 51 Erichsonella filiformis/floridana 26

19 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Chassahowitzka Sphaeromatidae Cassidinidea ovalis 1 Harrieta faxoni 46 Isopoda sp. B 1 Paracerceis caudata 93 Tanaidacea Kalliapseudidae Mesokalliapseudes macsweenyi 1 Leptocheliidae Leptochelia forresti 2 Leptochelia rapax 12 Metapseudidae Hoplomachus propinquus 3 Echinodermata Asteroidea Spinulosida Echinasteridae Echinaster serpentarius 2 Ophiuroidea Ophiurida Amphiuridae Amphioplus abditus 95 Mollusca Bivalvia Anomalodesmata Lyonsiidae Lyonsia floridana 3 Carditoida Carditidae Cardites floridanus 21 Lucinoida Lucinidae Codakia orbicularis 24 Lucinisca nassula 14 Mytiloida Mytilidae Brachidontes exustus 14 Veneroida Cardiidae Laevicardium mortoni 1 Montacutidae Mysella planulata 73 Veneridae Chione elevata 58

20 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Chassahowitzka Transennella conradina 4 Gastropoda -- Pyramidellidae Longchaeus suturalis 18 Turbonilla sp. 1 Caenogastropoda Cerithiidae Bittiolum varium 1 Cerithium atratum 5 Cerithium muscarum 475 Modulidae Modulus modulus 800 Cephalaspidea Acteocinidae Acteocina candei 5 Bullidae Bulla occidentalis 396 Haminoeidae Haminoea sp. A 6 Littorinimorpha Caecidae Caecum pulchellum 32 Calyptraeidae Crepidula plana complex 1 Crepidula spp. 458 Eulimidae Vitreolina conica 2 Rissoinidae Schwartziella catesbyana 759 Tornidae Teinostoma cocolitoris 1 Vitrinella floridana 7 Neogastropoda Columbellidae Astyris lunata 24 Columbella rusticoides 1 Costoanachis semiplicata 108 Drilliidae Cerodrillia spp. 3 Fasciolariidae Cinctura hunteria 2 Mangeliidae Pyrgocythara filosa 56

21 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Chassahowitzka Pyrgocythara hemphilli 16 Rubellatoma diomedea 6 Marginellidae Dentimargo eburneolus 14 Granulina hadria 35 Prunum apicinum 270 Prunum succineum 4 Muricidae Urosalpinx tampaensis 2 Nassariidae Nassarius albus/concensus 1 Nassarius vibex 24 Olivellidae Olivella spp. 79 Pseudomelatomidae Pilsbryspira leucocyma 128 Terebridae Terebra protexta 2 Nudibranchia -- Nudibranchia sp. A 34 Sacoglossa Volvatellidae Ascobulla ulla 1 Polyplacophora Chitonida Ischnochitonidae Ischnochiton papillosus 438 Sipuncula Sipunculidae Golfingiida Phascolionidae Phascolion sp. 211

22 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Homosassa Annelida Polychaeta -- -- Polychaete spp. 35 Amphinomida Amphinomidae Amphinomidae sp. 3 Eunicida Eunicidae Eunicidae sp. A 60 Eunicidae sp. B 176 Lumbrineridae Lumbrineridae sp. A 11 Arthropoda Malacostraca Amphipoda -- Gammaridea spp. 27 Ampeliscidae Ampelisca abdita 30 Ampithoidae Ampithoe longimana 32 Cymadusa compta 388 Aoridae Bemlos spinicarpus 7 Bateidae Batea cuspidata 219 Caprellidae Paracaprella tenuis 1 Gammaridae Gammarus mucronatus 67 Ischyroceridae Ericthonius punctatus 4 Lysianassidae Lysianopsis alba 1215 Maeridae Elasmopus levis 120 Melitidae Dulichiella appendiculata 62 Phoxocephalidae Phoxocephalidae sp. 1 Decapoda Alpheidae

23 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Homosassa Alpheus heterochaelis 140 Alpheus packardii 16 Epialtidae Libinia dubia 4 Hippolytidae Hippolyte pleuracantha 443 Hippolyte sp. 91 Hippolyte zostericola 344 Thor dobkini 91 Tozeuma carolinense 31 Paguridae Pagurus maclaughlinae 287 Palaemonidae Palaemon floridanus 77 Palaemonetes pugio 2 Panopeidae Neopanope packardii 168 Penaeidae Penaeus duorarum 12 Pinnotheridae Pinnixa cylindrica 20 Isopoda -- Idotea sp. A 3 Anthuridae Cyathura polita 8 Idoteidae Erichsonella attenuata 29 Erichsonella filiformis/floridana 4 Sphaeromatidae Harrieta faxoni 3 Isopoda sp. B 65 Paracerceis caudata 254 Tanaidacea Leptocheliidae Leptochelia forresti 4 Leptochelia rapax 48 Metapseudidae Hoplomachus propinquus 28 Pycnogonida -- -- Pycnogonida 1 Echinodermata

24 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Homosassa Asteroidea Spinulosida Echinasteridae Echinaster serpentarius 42 Holothuroidea Aspidochirotida Holothuriidae Holothuriidae sp. 4 Ophiuroidea Ophiurida Amphiuridae Amphioplus abditus 40 Ophiodermatidae Ophioderma brevispina 20 Mollusca Bivalvia Arcoida Noetiidae Arcopsis adamsi 6 Carditoida Carditidae Cardites floridanus 363 Lucinoida Lucinidae Codakia orbicularis 11 Lucinisca nassula 9 Mytiloida Mytilidae Brachidontes exustus 3 Modiolus squamosus 1 Musculus lateralis 1 Nuculida Nuculidae Nucula proxima 4 Pterioida Pteriidae Pteria colymbus 8 Veneroida Montacutidae Mysella planulata 45 Tellinidae Tellina texana 5 Veneridae Chione elevata 36

25 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Homosassa Gastropoda -- 1 Pyramidellidae Sayella laevigata 25 Turbonilla sp. 15 Turbinidae Turbo castanea 21 Amphipoda Phasianellidae Eulithidium thalassicola 9 Caenogastropoda Cerithiidae Bittiolum varium 2 Cerithium atratum 12 Cerithium muscarum 15 Modulidae Modulus modulus 142 Turritellidae Vermicularia knorrii 5 Cephalaspidea Bullidae Bulla occidentalis 136 Haminoeidae Haminoea sp. A 13 Littorinimorpha Caecidae Caecum pulchellum 6 32 Calyptraeidae Crepidula spp. 61 Eulimidae Vitreolina conica 38 Rissoinidae Phosinella cancellata complex 135 Schwartziella catesbyana 718 Tornidae Cyclostremiscus suppressus 3 Teinostoma cocolitoris 1 Vitrinella floridana 12 Neogastropoda Columbellidae Astyris lunata 64

26 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Homosassa Columbella rusticoides 15 Costoanachis semiplicata 125 Conidae Conasprella spp. 4 Costellariidae Mitromica foveata 9 exiguum 11 Fasciolariidae Cinctura hunteria 5 tulipa 1 Mangeliidae Pyrgocythara filosa 54 Pyrgocythara hemphilli 11 14 Rubellatoma diomedea 2 Marginellidae Dentimargo eburneolus 73 Granulina hadria 52 Prunum apicinum 357 Prunum succineum 7 Muricidae 3 2 Nassariidae Nassarius albus/concensus 17 Nassarius vibex 20 Olivellidae Olivella spp. 22 Pseudomelatomidae Pilsbryspira leucocyma 133 tampaensis 4 Nudibranchia -- Nudibranchia sp. A 12 Sacoglossa Volvatellidae Ascobulla ulla 15 Polyplacophora Chitonida Chaetopleuridae Chaetopleura apiculata 3 Ischnochitonidae Ischnochiton papillosus 1521 Sipuncula

27 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Homosassa Sipunculidae Golfingiida Phascolionidae Phascolion sp. 107

28 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Crystal Annelida Polychaeta -- -- Polychaete spp. 26 Amphinomida Amphinomidae Amphinomidae sp. 2 Eunicida Eunicidae Eunicidae sp. A 30 Eunicidae sp. B 90 Lumbrineridae Lumbrineridae sp. A 4 Onuphidae Diopatra sp. 7 Arthropoda Malacostraca Amphipoda -- Gammaridea spp. 48 Ampeliscidae Ampelisca abdita 50 Ampithoidae Ampithoe longimana 9 Cymadusa compta 353 Aoridae Bemlos spinicarpus 3 Globosolembos smithi 14 Grandidierella bonnieroides 9 Bateidae Batea cuspidata 23 Caprellidae Paracaprella tenuis 8 Corophiidae Apocorophium simile 3 Gammaridae Gammarus mucronatus 6 Ischyroceridae Ericthonius punctatus 6 Lysianassidae Lysianopsis alba 182 Maeridae Elasmopus levis 340

29 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Crystal Melitidae Dulichiella appendiculata 12 Phoxocephalidae Eobrolgus spinosus 4 Phoxocephalidae sp. 2 Decapoda Alpheidae Alpheus heterochaelis 14 Alpheus packardii 134 Alpheus sp. 2 Epialtidae Epialtus dilatatus 1 Libinia dubia 16 Pelia mutica 15 Pitho spp. 2 Hippolytidae Hippolyte pleuracantha 337 Hippolyte sp. 71 Hippolyte zostericola 314 Latreutes fucorum 4 Thor dobkini 66 Tozeuma carolinense 25 Paguridae Pagurus maclaughlinae 454 Palaemonidae Palaemon floridanus 136 Palaemonetes pugio 11 Panopeidae Neopanope packardii 414 Penaeidae Penaeus duorarum 18 Pinnotheridae Pinnixa cylindrica 12 Portunidae Callinectes sapidus 1 Isopoda Anthuridae Cyathura polita 8 Idoteidae Edotia triloba 1 Erichsonella attenuata 17 Erichsonella filiformis/floridana 3 Sphaeromatidae Harrieta faxoni 3

30 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Crystal Isopoda sp. A 1 Paracerceis caudata 49 Tanaidacea Leptocheliidae Leptochelia rapax 6 Metapseudidae Hoplomachus propinquus 1 Echinodermata Asteroidea Spinulosida Echinasteridae Echinaster serpentarius 23 Holothuroidea Aspidochirotida Holothuriidae Holothuriidae sp. 8 Ophiuroidea Ophiurida Amphiuridae Amphioplus abditus 15 Ophiodermatidae Ophioderma brevispina 21 Mollusca Bivalvia Arcoida Noetiidae Arcopsis adamsi 1 Carditoida Carditidae Cardites floridanus 133 Lucinoida Lucinidae Codakia orbicularis 19 Lucinisca nassula 32 Mytiloida Mytilidae Brachidontes exustus 32 Modiolus squamosus 1 Musculus lateralis 7 Nuculida Nuculidae Nucula proxima 24 Veneroida Cardiidae

31 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Crystal Laevicardium mortoni 3 Montacutidae Mysella planulata 37 Tellinidae Ameritella versicolor 14 Tellina texana 14 Veneridae Chione elevata 32 Transennella conradina 1 Transennella stimpsoni 1 Gastropoda -- Pyramidellidae Longchaeus suturalis 37 Sayella laevigata 15 Turbonilla sp. 36 Amphipoda Phasianellidae Eulithidium thalassicola 12 Caenogastropoda Cerithiidae Bittiolum varium 44 Cerithium muscarum 7 Cerithiopsidae Cerithiopsis sp. 2 Modulidae Modulus modulus 152 Cephalaspidea Acteocinidae Acteocina candei 52 Bullidae Bulla occidentalis 82 Haminoeidae Haminoea sp. A 10 Littorinimorpha Caecidae Caecum pulchellum 12 Meioceras nitidum 1739 Calyptraeidae Crepidula spp. 170 Eulimidae Melanella sp. 2 Vitreolina conica 2 Rissoinidae

32 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Crystal Schwartziella catesbyana 961 Tornidae Cyclostremiscus suppressus 32 Vitrinella floridana 12 Neogastropoda Columbellidae Astyris lunata 766 Columbella rusticoides 21 Costoanachis semiplicata 475 Conidae Conasprella spp. 15 Costellariidae Mitromica foveata 3 Drilliidae Cerodrillia spp. 3 Fasciolariidae Cinctura hunteria 3 Mangeliidae Pyrgocythara filosa 26 Pyrgocythara hemphilli 51 Rubellatoma diomedea 2 Marginellidae Dentimargo eburneolus 99 Granulina hadria 8 Prunum apicinum 571 Prunum succineum 7 Muricidae Eupleura sulcidentata 1 Urosalpinx perrugata 1 Urosalpinx tampaensis 2 Nassariidae Nassarius albus/concensus 1 Nassarius vibex 32 Olivellidae Olivella spp. 48 Pseudomelatomidae Pilsbryspira leucocyma 157 Pyrgospira tampaensis 2 Terebridae Terebra protexta 2 Nudibranchia -- Nudibranchia sp. A 19 Sacoglossa

33 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Crystal Volvatellidae Ascobulla ulla 13 Polyplacophora Chitonida Ischnochitonidae Ischnochiton papillosus 1191 Sipuncula Sipunculidae Golfingiida Phascolionidae Phascolion sp. 703

34 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Waccasassa Annelida Polychaeta -- -- Polychaete spp. 23 Amphinomida Amphinomidae Amphinomidae sp. 2 Eunicida Eunicidae Eunicidae sp. A 37 Eunicidae sp. B 50 Lumbrineridae Lumbrineridae sp. A 38 Onuphidae Diopatra sp. 26 Phyllodocida Polynoidae Polynoidae sp. A 4 Arthropoda Malacostraca Amphipoda -- Gammaridea spp. 22 Ampeliscidae Ampelisca abdita 19 Ampithoidae Ampithoe longimana 13 Cymadusa compta 271 Aoridae Bemlos spinicarpus 1 Globosolembos smithi 16 Caprellidae Caprella penantis 1 Paracaprella tenuis 6 Corophiidae Apocorophium simile 1 Monocorophium acherusicum 1 Gammaridae Gammarus mucronatus 1 Ischyroceridae Ericthonius punctatus 4 Lysianassidae Lysianopsis alba 23

35 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Waccasassa Maeridae Elasmopus levis 81 Phoxocephalidae Eobrolgus spinosus 44 Phoxocephalidae sp. 1 Decapoda Alpheidae Alpheus heterochaelis 18 Alpheus packardii 61 Alpheus sp. 6 Epialtidae Libinia dubia 11 Pelia mutica 5 Hippolytidae Hippolyte pleuracantha 198 Hippolyte sp. 39 Hippolyte zostericola 262 Latreutes fucorum 1 Thor dobkini 3 Tozeuma carolinense 50 Inachidae Metoporhaphis calcarata 1 Paguridae Pagurus maclaughlinae 1203 Palaemonidae Palaemon floridanus 24 Palaemonetes pugio 4 Panopeidae Neopanope packardii 150 Penaeidae Penaeus duorarum 28 Pinnotheridae Pinnixa cylindrica 3 Isopoda Anthuridae Cyathura polita 26 Idoteidae Edotia triloba 6 Erichsonella attenuata 33 Sphaeromatidae Harrieta faxoni 9 Paracerceis caudata 107 Tanaidacea Leptocheliidae

36 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Waccasassa Leptochelia rapax 4 Echinodermata Asteroidea Spinulosida Echinasteridae Echinaster serpentarius 17 Ophiuroidea Ophiurida Amphiuridae Amphioplus abditus 42 Ophiodermatidae Ophioderma brevispina 25 Mollusca Bivalvia Carditoida Carditidae Cardites floridanus 189 Lucinoida Lucinidae Codakia orbicularis 13 Lucinisca nassula 1 Mytiloida Mytilidae Brachidontes exustus 11 Musculus lateralis 1 Nuculida Nuculidae Nucula proxima 4 Veneroida Cardiidae Laevicardium mortoni 22 Montacutidae Mysella planulata 28 Tellinidae Ameritella versicolor 3 Psammotreta intastriata 3 Tellina texana 8 Veneridae Chione elevata 36 Transennella conradina 56 Transennella stimpsoni 1 Gastropoda -- Pyramidellidae

37 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Waccasassa Boonea impressa 5 Longchaeus suturalis 4 Sayella laevigata 5 Turbonilla sp. 126 Caenogastropoda Cerithiidae Bittiolum varium 174 Cerithium muscarum 50 Cerithiopsidae Cerithiopsis sp. 19 Modulidae Modulus modulus 216 Cephalaspidea Acteocinidae Acteocina candei 523 Bullidae Bulla occidentalis 141 Haminoeidae Haminoea elegans 22 Haminoea sp. A 11 Littorinimorpha Caecidae Caecum pulchellum 79 Meioceras nitidum 2 Calyptraeidae Crepidula plana complex 2 Crepidula spp. 965 Eulimidae Melanella sp. 2 Vitreolina conica 3 Rissoinidae Schwartziella catesbyana 728 Tornidae Cyclostremiscus suppressus 43 Vitrinella floridana 4 Neogastropoda Columbellidae Astyris lunata 3635 Columbella rusticoides 21 Costoanachis semiplicata 877 Parvanachis ostreicola 1 Conidae Conasprella spp. 13 Mangeliidae

38 Supplementary Materials

Total System Phylum: Class: Order: Genus: Species Abundance Waccasassa Pyrgocythara filosa 1 Pyrgocythara hemphilli 195 Marginellidae Dentimargo eburneolus 197 Granulina hadria 15 Prunum apicinum 638 Muricidae Urosalpinx cinerea 3 Urosalpinx perrugata 2 Nassariidae Nassarius vibex 151 Olivellidae Olivella spp. 232 Pseudomelatomidae Pilsbryspira leucocyma 3 Pyrgospira tampaensis 1 Sacoglossa Volvatellidae Ascobulla ulla 1 Polyplacophora Chitonida Ischnochitonidae Ischnochiton papillosus 5 Sipuncula Sipunculidae Golfingiida Phascolionidae Phascolion sp. 741

39 Supplementary Materials

3 Table S2 PERMDISP Results

3.1 Table S2.1 Species-level PERMDISP for system and stations within systems. Bold italics indicate significance at the 0.05 level or below.

df SS MS F p System 4 0.073 0.018 4.478 0.028 Residuals 70 0.286 0.004

df SS MS F p Stations within WEE 4 0.039 0.010 1.130 0.396 Residuals 10 0.087 0.009

df SS MS F p Stations within CHA 4 0.020 0.005 1.171 0.380 Residuals 10 0.043 0.004

df SS MS F p Stations within HOM 4 0.007 0.002 0.384 0.816 Residuals 10 0.044 0.004

df SS MS F p Stations within CRY 4 0.012 0.003 1.019 0.443 Residuals 10 0.029 0.003

df SS MS F p Stations within WAC 4 0.005 0.001 0.262 0.896 Residuals 10 0.046 0.004

3.2 Table S2.2 Trophic role PERMDISP for system and stations within systems. Bold italics indicate significance at the 0.05 level or below.

df SS MS F p System 4 0.063 0.016 2.626 0.042 Residuals 70 0.419 0.006

df SS MS F p Stations within WEE 4 0.005 0.001 0.334 0.849 Residuals 10 0.041 0.004

df SS MS F p Stations within CHA 4 0.014 0.003 0.588 0.679 Residuals 10 0.058 0.006

40 Supplementary Materials

df SS MS F p Stations within HOM 4 0.016 0.004 0.381 0.818 Residuals 10 0.105 0.011

df SS MS F p Stations within CRY 4 0.013 0.003 1.063 0.424 Residuals 10 0.030 0.003

df SS MS F p Stations within WAC 4 0.013 0.003 0.990 0.456 Residuals 10 0.034 0.003

3.3 Table S2.3 Species-level Tukey's multiple pairwise comparisons adjusted to achieve a 95% family-wise confidence level. Italics indicate significance at the 0.05 level or below. System Comparison Difference Lower CL Upper CL Adjusted p CRY-CHA 0.011 -0.054 0.077 0.988 HOM-CHA -0.032 -0.097 0.033 0.646 WAC-CHA -0.074 -0.140 -0.009 0.018 WEE-CHA 0.000 -0.065 0.066 1.000 HOM-CRY -0.044 -0.109 0.022 0.345 WAC-CRY -0.086 -0.151 -0.020 0.004 WEE-CRY -0.011 -0.077 0.054 0.989 WAC-HOM -0.042 -0.107 0.023 0.380 WEE-HOM 0.032 -0.033 0.098 0.639 WEE-WAC 0.074 0.009 0.140 0.018

3.4 Table S2.4 Trophic role Tukey's multiple pairwise comparisons adjusted to achieve a 95% family-wise confidence level. Italics indicate significance at the 0.05 level or below. System Comparison Difference Lower CL Upper CL Adjusted p CRY-CHA 0.038 -0.041 0.118 0.654 HOM-CHA -0.013 -0.092 0.067 0.992 WAC-CHA -0.047 -0.126 0.032 0.462 WEE-CHA -0.029 -0.108 0.050 0.840 HOM-CRY -0.051 -0.130 0.028 0.379 WAC-CRY -0.086 -0.165 -0.006 0.028 WEE-CRY -0.068 -0.147 0.012 0.129 WAC-HOM -0.035 -0.114 0.045 0.739 WEE-HOM -0.017 -0.096 0.063 0.976 WEE-WAC 0.018 -0.061 0.097 0.969

41 Supplementary Materials

4 Table S3.1 Species-level SIMPER results. Comparisons were made across adjacent estuarine systems, distal systems, and between each distal system and the central system. Comparisons were also made across aboveground biomass categories (high vs. low). Abundances are double-Wisconsin-standardized values. Asterisks indicate significance level: <0.001 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1.

4.1 Weeki Wachee (WEE) vs. Chassahowitzka (CHA)

Average Average Contribution Abundance Abundance Cumulative WEE vs. CHA % WEE CHA % p Caecum pulchellum 0.018 0.038 0.002 0.026 0.001 *** Laevicardium mortoni 0.016 0.033 0.000 0.050 0.001 *** Amphioplus abditus 0.015 0.033 0.041 0.072 0.019 * Polychaete spp. 0.015 0.029 0.009 0.094 0.003 ** Alpheus heterochaelis 0.015 0.013 0.043 0.116 0.006 ** Modulus modulus 0.015 0.009 0.036 0.137 0.001 *** Nassarius vibex 0.015 0.035 0.007 0.159 0.002 ** Bulla occidentalis 0.014 0.035 0.035 0.179 0.021 * Erichsonella attenuata 0.013 0.019 0.020 0.198 0.001 *** Lumbrineridae sp. A 0.013 0.012 0.027 0.217 0.001 *** Nassarius albus concensus 0.013 0.026 0.001 0.235 0.001 *** Schwartziella catesbyana 0.012 0.027 0.007 0.252 0.002 ** Ampelisca abdita 0.012 0.022 0.034 0.268 0.142 Cerithium muscarum 0.011 0.011 0.030 0.285 0.001 *** Grandidierella bonnieroides 0.011 0.025 0.003 0.301 0.001 *** Callinectes sapidus 0.011 0.006 0.019 0.317 0.033 * Mysella planulata 0.011 0.001 0.022 0.333 0.015 * Batea cuspidata 0.011 0.002 0.022 0.348 0.137 Cyathura polita 0.011 0.018 0.023 0.364 0.250 Pyrgocythara filosa 0.011 0.004 0.023 0.379 0.054 . Eunicidae sp. A 0.011 0.022 0.012 0.394 0.004 ** Pilsbryspira leucocyma 0.010 0.019 0.024 0.409 0.205 Cerithium atratum 0.010 0.015 0.008 0.424 0.109 Libinia dubia 0.009 0.013 0.013 0.437 0.756 Gammaridea spp. 0.009 0.010 0.014 0.450 0.174 Codakia orbicularis 0.009 0.014 0.012 0.463 0.004 ** Pinnixa cylindrica 0.009 0.013 0.011 0.476 0.012 * Monocorophium acherusicum 0.009 0.011 0.011 0.488 0.002 ** Eunicidae sp. B 0.009 0.025 0.020 0.501 0.067 . Chione elevata 0.008 0.001 0.018 0.513 0.001 *** Brachidontes exustus 0.008 0.018 0.001 0.525 0.001 *** Erichsonella filiformis floridana 0.008 0.005 0.016 0.536 0.001 *** Edotia triloba 0.008 0.014 0.004 0.548 0.134 Hippolyte pleuracantha 0.008 0.013 0.021 0.560 0.572 Ampithoe longimana 0.008 0.014 0.006 0.571 0.322

42 Supplementary Materials

Pagurus maclaughlinae 0.008 0.029 0.017 0.582 0.022 * Tozeuma carolinense 0.008 0.013 0.009 0.593 0.955 Thor dobkini 0.008 0.010 0.010 0.604 0.350 Prunum apicinum 0.007 0.015 0.021 0.615 0.935 Vitrinella floridana 0.007 0.012 0.005 0.625 0.155 Nudibranchia sp. A 0.007 0.000 0.014 0.636 0.028 * Paracerceis caudata 0.007 0.002 0.014 0.646 0.754 Lysianopsis alba 0.007 0.014 0.009 0.656 0.678 Neopanope packardii 0.007 0.019 0.006 0.665 0.690 Mesokalliapseudes macsweenyi 0.007 0.011 0.003 0.675 0.013 * Olivella spp. 0.006 0.006 0.013 0.684 0.713 Lucinisca nassula 0.006 0.000 0.013 0.693 0.382 Cymadusa compta 0.006 0.018 0.022 0.703 0.784 Harrieta faxoni 0.006 0.010 0.010 0.712 0.002 ** Lyonsia floridana 0.006 0.011 0.002 0.721 0.002 ** Ischnochiton papillosus 0.006 0.002 0.013 0.729 0.997 Transennella stimpsoni 0.006 0.012 0.000 0.738 0.010 ** Crepidula plana complex 0.006 0.006 0.006 0.746 0.261 Alpheus sp. 0.006 0.005 0.008 0.754 0.210 Amphinomidae sp. 0.006 0.010 0.002 0.762 0.236 Hippolyte zostericola 0.006 0.007 0.016 0.770 0.992 Penaeus duorarum 0.005 0.006 0.008 0.778 0.996 Cerodrillia spp. 0.005 0.006 0.006 0.786 0.032 * Rubellatoma diomedea 0.005 0.001 0.009 0.793 0.155 Pitho spp. 0.005 0.009 0.004 0.800 0.001 *** Longchaeus suturalis 0.005 0.000 0.010 0.807 0.219 0.005 0.010 0.000 0.814 0.001 *** Apocorophium simile 0.005 0.001 0.009 0.821 0.156 Crepidula spp. 0.005 0.008 0.013 0.828 0.721 Haminoea sp. A 0.005 0.000 0.009 0.834 0.931 Granulina hadria 0.005 0.001 0.009 0.841 0.345 Transennella conradina 0.005 0.009 0.001 0.848 0.116 Urosalpinx tampaensis 0.005 0.005 0.006 0.854 0.062 . Ascobulla ulla 0.005 0.009 0.001 0.861 0.792 Phoxocephalidae sp. 0.004 0.000 0.009 0.867 0.111 Leptochelia forresti 0.004 0.003 0.006 0.874 0.338 Gammarus mucronatus 0.004 0.000 0.009 0.880 0.343 Hippolyte sp. 0.004 0.002 0.008 0.886 0.831 Leptochelia rapax 0.004 0.002 0.008 0.892 0.857 Cinctura hunteria 0.004 0.005 0.005 0.898 0.628 Dentimargo eburneolus 0.004 0.008 0.004 0.904 1.000 Alpheus packardii 0.004 0.008 0.000 0.910 0.996 Idotea sp. A 0.004 0.007 0.002 0.916 0.150 Prunum succineum 0.004 0.000 0.008 0.922 0.667 Palaemon floridanus 0.004 0.001 0.008 0.927 0.937

43 Supplementary Materials

Ericthonius punctatus 0.004 0.004 0.004 0.932 0.771 Costoanachis semiplicata 0.003 0.004 0.006 0.937 1.000 Ophioderma brevispina 0.003 0.007 0.000 0.942 0.956 Terebra protexta 0.003 0.005 0.002 0.947 0.060 . Ameritella versicolor 0.003 0.006 0.000 0.952 0.649 Sayella laevigata 0.003 0.006 0.000 0.957 0.963 Cerithiopsis sp. 0.003 0.006 0.000 0.961 0.661 Bittiolum varium 0.003 0.006 0.000 0.965 0.953 Elasmopus levis 0.003 0.008 0.006 0.969 0.998 Astyris lunata 0.003 0.006 0.000 0.973 1.000 Phascolion sp. 0.002 0.002 0.005 0.977 0.984 Pyrgocythara hemphilli 0.002 0.004 0.004 0.980 1.000 Palaemonetes pugio 0.002 0.003 0.000 0.983 0.857 Vitreolina conica 0.002 0.003 0.001 0.985 0.937 Bemlos spinicarpus 0.002 0.000 0.003 0.987 0.880 Columbella rusticoides 0.001 0.002 0.001 0.989 1.000 Diopatra sp. 0.001 0.002 0.001 0.991 1.000 Globosolembos smithi 0.001 0.002 0.000 0.993 0.975 Tellina texana 0.001 0.002 0.000 0.994 0.996 Cardites floridanus 0.001 0.000 0.002 0.995 1.000 Echinaster serpentarius 0.001 0.000 0.001 0.996 1.000 Hoplomachus propinquus 0.001 0.000 0.001 0.997 0.960 Turbonilla sp. 0.000 0.001 0.000 0.998 1.000 Acteocina candei 0.000 0.001 0.000 0.998 1.000 Pelia mutica 0.000 0.001 0.000 0.999 0.985 Conasprella spp. 0.000 0.001 0.000 0.999 1.000 Cyclostremiscus suppressus 0.000 0.000 0.000 0.999 0.998 Dulichiella appendiculata 0.000 0.000 0.000 1.000 0.998 Isopoda sp. B 0.000 0.000 0.000 1.000 0.892 Meioceras nitidum 0.000 0.000 0.000 1.000 0.999 Boonea impressa 0.000 0.000 0.000 1.000 1.000 Eobrolgus spinosus 0.000 0.000 0.000 1.000 1.000 Eulithidium thalassicola 0.000 0.000 0.000 1.000 1.000 Haminoea elegans 0.000 0.000 0.000 1.000 1.000 Holothuriidae sp. 0.000 0.000 0.000 1.000 1.000 Latreutes fucorum 0.000 0.000 0.000 1.000 1.000 Melanella sp. 0.000 0.000 0.000 1.000 1.000 Mitromica foveata 0.000 0.000 0.000 1.000 1.000 Musculus lateralis 0.000 0.000 0.000 1.000 1.000 Nucula proxima 0.000 0.000 0.000 1.000 1.000 Paracaprella tenuis 0.000 0.000 0.000 1.000 1.000 Phosinella cancellata complex 0.000 0.000 0.000 1.000 0.988 Pteria colymbus 0.000 0.000 0.000 1.000 1.000 Pyrgocythara plicosa 0.000 0.000 0.000 1.000 1.000 Pyrgospira tampaensis 0.000 0.000 0.000 1.000 1.000

44 Supplementary Materials

Turbo castanea 0.000 0.000 0.000 1.000 1.000 Urosalpinx cinerea 0.000 0.000 0.000 1.000 1.000 Urosalpinx perrugata 0.000 0.000 0.000 1.000 1.000 Vermicularia knorrii 0.000 0.000 0.000 1.000 1.000 Vexillum exiguum 0.000 0.000 0.000 1.000 1.000

4.2 Chassahowitzka (CHA) vs. Homosassa (HOM)

Average Average Contribution Abundance Abundance Cumulative CHA vs. HOM % CHA HOM % p Modulus modulus 0.016 0.036 0.005 0.023 0.001 *** Ampelisca abdita 0.015 0.034 0.009 0.045 0.001 *** Cerithium muscarum 0.015 0.030 0.001 0.067 0.001 *** Amphioplus abditus 0.014 0.041 0.013 0.088 0.081 . Bulla occidentalis 0.013 0.035 0.009 0.108 0.055 . Alpheus heterochaelis 0.013 0.043 0.031 0.127 0.111 Batea cuspidata 0.013 0.022 0.032 0.147 0.001 *** Ischnochiton papillosus 0.013 0.013 0.036 0.166 0.004 ** Paracerceis caudata 0.013 0.014 0.033 0.185 0.001 *** Lumbrineridae sp. A 0.013 0.027 0.004 0.203 0.001 *** Lysianopsis alba 0.011 0.009 0.030 0.220 0.008 ** Pyrgocythara filosa 0.011 0.023 0.018 0.237 0.018 * Pyrgocythara plicosa 0.011 0.000 0.022 0.253 0.001 *** Echinaster serpentarius 0.011 0.001 0.023 0.269 0.001 *** Mysella planulata 0.011 0.022 0.010 0.286 0.011 * Leptochelia rapax 0.011 0.008 0.026 0.302 0.002 ** Pilsbryspira leucocyma 0.011 0.024 0.019 0.318 0.139 Cyathura polita 0.010 0.023 0.007 0.333 0.340 Haminoea sp. A 0.010 0.009 0.016 0.347 0.162 Erichsonella attenuata 0.010 0.020 0.008 0.362 0.332 Callinectes sapidus 0.009 0.019 0.000 0.375 0.085 Cerithium atratum 0.009 0.008 0.016 0.389 0.141 Cardites floridanus 0.009 0.002 0.020 0.403 0.001 *** Hippolyte pleuracantha 0.009 0.021 0.030 0.417 0.224 Thor dobkini 0.009 0.010 0.014 0.430 0.094 . Tozeuma carolinense 0.009 0.009 0.016 0.443 0.637 Gammarus mucronatus 0.008 0.009 0.011 0.455 0.001 *** Erichsonella filiformis floridana 0.008 0.016 0.002 0.467 0.001 *** Ampithoe longimana 0.008 0.006 0.017 0.478 0.321 Libinia dubia 0.008 0.013 0.006 0.489 0.955 Gammaridea spp. 0.008 0.014 0.006 0.501 0.479 Cymadusa compta 0.008 0.022 0.027 0.512 0.361 Nudibranchia sp. A 0.007 0.014 0.004 0.523 0.010 ** Prunum apicinum 0.007 0.021 0.021 0.533 0.971

45 Supplementary Materials

Lucinisca nassula 0.007 0.013 0.005 0.544 0.232 Granulina hadria 0.007 0.009 0.010 0.555 0.003 ** Eunicidae sp. B 0.007 0.020 0.024 0.565 0.577 Hippolyte zostericola 0.007 0.016 0.028 0.576 0.860 Prunum succineum 0.007 0.008 0.010 0.586 0.022 * Leptochelia forresti 0.007 0.006 0.009 0.596 0.044 * Sayella laevigata 0.007 0.000 0.014 0.606 0.020 * Dentimargo eburneolus 0.007 0.004 0.017 0.616 0.995 Polychaete spp. 0.007 0.009 0.006 0.626 0.736 Vitreolina conica 0.007 0.001 0.013 0.636 0.001 *** Dulichiella appendiculata 0.007 0.000 0.013 0.646 0.001 *** Ascobulla ulla 0.007 0.001 0.013 0.656 0.267 Pinnixa cylindrica 0.007 0.011 0.008 0.665 0.294 Chione elevata 0.006 0.018 0.008 0.675 0.029 * Olivella spp. 0.006 0.013 0.002 0.684 0.751 Penaeus duorarum 0.006 0.008 0.010 0.694 0.990 Hippolyte sp. 0.006 0.008 0.011 0.703 0.392 Cinctura hunteria 0.006 0.005 0.010 0.712 0.195 Vermicularia knorrii 0.006 0.000 0.012 0.721 0.004 ** Pagurus maclaughlinae 0.006 0.017 0.005 0.730 0.620 Crepidula spp. 0.006 0.013 0.001 0.738 0.390 Hoplomachus propinquus 0.006 0.001 0.011 0.746 0.001 *** Pteria colymbus 0.005 0.000 0.011 0.754 0.001 *** Rubellatoma diomedea 0.005 0.009 0.003 0.762 0.107 Monocorophium acherusicum 0.005 0.011 0.000 0.770 0.294 Vexillum exiguum 0.005 0.000 0.010 0.778 0.001 *** Eunicidae sp. A 0.005 0.012 0.006 0.785 0.758 Bemlos spinicarpus 0.005 0.003 0.008 0.793 0.073 . Harrieta faxoni 0.005 0.010 0.000 0.800 0.111 Longchaeus suturalis 0.005 0.010 0.000 0.808 0.198 Columbella rusticoides 0.005 0.001 0.009 0.815 0.916 Palaemon floridanus 0.005 0.008 0.010 0.822 0.858 Phoxocephalidae sp. 0.005 0.009 0.001 0.828 0.070 . Vitrinella floridana 0.005 0.005 0.007 0.835 0.683 Codakia orbicularis 0.005 0.012 0.004 0.842 0.667 Urosalpinx perrugata 0.004 0.000 0.009 0.848 0.358 Apocorophium simile 0.004 0.009 0.000 0.855 0.279 Ophioderma brevispina 0.004 0.000 0.008 0.861 0.829 Neopanope packardii 0.004 0.006 0.013 0.867 0.991 Eulithidium thalassicola 0.004 0.000 0.008 0.873 0.169 Costoanachis semiplicata 0.004 0.006 0.005 0.879 1.000 Ericthonius punctatus 0.004 0.004 0.005 0.884 0.722 Alpheus sp. 0.004 0.008 0.000 0.890 0.498 Mitromica foveata 0.004 0.000 0.008 0.895 0.033 * Nassarius vibex 0.004 0.007 0.005 0.901 1.000

46 Supplementary Materials

Isopoda sp. B 0.004 0.000 0.007 0.906 0.001 *** Pyrgospira tampaensis 0.003 0.000 0.007 0.911 0.138 Schwartziella catesbyana 0.003 0.007 0.004 0.916 0.989 Elasmopus levis 0.003 0.006 0.009 0.921 0.985 Idotea sp. A 0.003 0.002 0.005 0.926 0.346 Nassarius albus concensus 0.003 0.001 0.006 0.930 0.933 Cerodrillia spp. 0.003 0.006 0.000 0.935 0.425 Crepidula plana complex 0.003 0.006 0.000 0.939 0.710 Urosalpinx tampaensis 0.003 0.006 0.000 0.943 0.329 Amphinomidae sp. 0.003 0.002 0.004 0.947 0.827 Turbo castanea 0.003 0.000 0.006 0.952 0.001 *** Urosalpinx cinerea 0.002 0.000 0.005 0.955 0.352 Phosinella cancellata complex 0.002 0.000 0.005 0.959 0.001 *** Phascolion sp. 0.002 0.005 0.002 0.962 0.989 Holothuriidae sp. 0.002 0.000 0.005 0.966 0.528 Tellina texana 0.002 0.000 0.005 0.969 0.901 Pyrgocythara hemphilli 0.002 0.004 0.002 0.972 1.000 Pitho spp. 0.002 0.004 0.000 0.975 0.624 Edotia triloba 0.002 0.004 0.000 0.978 0.987 Mesokalliapseudes macsweenyi 0.002 0.003 0.000 0.980 0.698 Alpheus packardii 0.002 0.000 0.003 0.982 1.000 Grandidierella bonnieroides 0.002 0.003 0.000 0.985 0.981 Conasprella spp. 0.001 0.000 0.003 0.987 0.974 Terebra protexta 0.001 0.002 0.000 0.989 0.621 Caecum pulchellum 0.001 0.002 0.000 0.990 1.000 Turbonilla sp. 0.001 0.000 0.002 0.992 1.000 Lyonsia floridana 0.001 0.002 0.000 0.993 0.680 Palaemonetes pugio 0.001 0.000 0.001 0.994 0.986 Brachidontes exustus 0.001 0.001 0.000 0.995 1.000 Diopatra sp. 0.001 0.001 0.000 0.996 1.000 Transennella conradina 0.000 0.001 0.000 0.996 1.000 Nucula proxima 0.000 0.000 0.001 0.997 0.908 Musculus lateralis 0.000 0.000 0.001 0.998 0.951 Astyris lunata 0.000 0.000 0.001 0.998 1.000 Paracaprella tenuis 0.000 0.000 0.001 0.999 0.966 Cyclostremiscus suppressus 0.000 0.000 0.000 0.999 0.989 Bittiolum varium 0.000 0.000 0.000 0.999 1.000 Laevicardium mortoni 0.000 0.000 0.000 1.000 1.000 Acteocina candei 0.000 0.000 0.000 1.000 1.000 Meioceras nitidum 0.000 0.000 0.000 1.000 0.997 Ameritella versicolor 0.000 0.000 0.000 1.000 1.000 Boonea impressa 0.000 0.000 0.000 1.000 1.000 Caecum imbricatum 0.000 0.000 0.000 1.000 1.000 Cerithiopsis sp. 0.000 0.000 0.000 1.000 1.000 Eobrolgus spinosus 0.000 0.000 0.000 1.000 1.000

47 Supplementary Materials

Globosolembos smithi 0.000 0.000 0.000 1.000 1.000 Haminoea elegans 0.000 0.000 0.000 1.000 1.000 Latreutes fucorum 0.000 0.000 0.000 1.000 1.000 Melanella sp. 0.000 0.000 0.000 1.000 1.000 Pelia mutica 0.000 0.000 0.000 1.000 1.000 Transennella stimpsoni 0.000 0.000 0.000 1.000 1.000

4.3 Homosassa (HOM) vs. Crystal (CRY)

Average Average Contribution Abundance Abundance Cumulative HOM vs. CRY % HOM CRY % p Alpheus packardii 0.017 0.003 0.036 0.024 0.001 *** Batea cuspidata 0.015 0.032 0.003 0.047 0.001 *** Alpheus heterochaelis 0.015 0.031 0.003 0.068 0.021 * Elasmopus levis 0.014 0.009 0.034 0.089 0.001 *** Paracerceis caudata 0.014 0.033 0.006 0.109 0.001 *** Neopanope packardii 0.013 0.013 0.037 0.128 0.001 *** Lysianopsis alba 0.013 0.030 0.004 0.147 0.001 *** Libinia dubia 0.013 0.006 0.026 0.166 0.069 . Leptochelia rapax 0.012 0.026 0.003 0.183 0.001 *** Haminoea sp. A 0.011 0.016 0.013 0.199 0.069 . Pyrgocythara plicosa 0.011 0.022 0.000 0.216 0.001 *** Costoanachis semiplicata 0.011 0.005 0.025 0.232 0.281 Prunum apicinum 0.011 0.021 0.038 0.248 0.183 Penaeus duorarum 0.011 0.010 0.020 0.264 0.314 Hippolyte zostericola 0.011 0.028 0.030 0.279 0.049 * Palaemon floridanus 0.010 0.010 0.021 0.294 0.001 *** Echinaster serpentarius 0.010 0.023 0.011 0.308 0.006 ** Tozeuma carolinense 0.009 0.016 0.014 0.322 0.484 Lucinisca nassula 0.009 0.005 0.019 0.336 0.004 ** Pyrgocythara filosa 0.009 0.018 0.009 0.349 0.228 Ischnochiton papillosus 0.009 0.036 0.031 0.362 0.831 Ascobulla ulla 0.009 0.013 0.011 0.376 0.011 * Cymadusa compta 0.009 0.027 0.027 0.389 0.034 * Hippolyte sp. 0.009 0.011 0.013 0.402 0.033 * Thor dobkini 0.009 0.014 0.010 0.414 0.161 Columbella rusticoides 0.008 0.009 0.017 0.427 0.013 * Hippolyte pleuracantha 0.008 0.030 0.025 0.439 0.538 Ampelisca abdita 0.008 0.009 0.017 0.451 0.836 Cerithium atratum 0.008 0.016 0.000 0.463 0.319 Pilsbryspira leucocyma 0.008 0.019 0.020 0.474 0.788 Ampithoe longimana 0.008 0.017 0.005 0.485 0.353 Gammaridea spp. 0.007 0.006 0.014 0.496 0.521 Dentimargo eburneolus 0.007 0.017 0.023 0.507 0.979

48 Supplementary Materials

Sayella laevigata 0.007 0.014 0.009 0.518 0.013 * Cinctura hunteria 0.007 0.010 0.006 0.528 0.086 . Cardites floridanus 0.007 0.020 0.008 0.538 0.110 Prunum succineum 0.007 0.010 0.008 0.549 0.035 * Ericthonius punctatus 0.007 0.005 0.011 0.559 0.184 Eunicidae sp. B 0.007 0.024 0.015 0.569 0.784 Vitreolina conica 0.007 0.013 0.001 0.579 0.001 *** Dulichiella appendiculata 0.007 0.013 0.003 0.588 0.001 *** Longchaeus suturalis 0.007 0.000 0.013 0.598 0.006 ** Conasprella spp. 0.006 0.003 0.012 0.608 0.163 Eulithidium thalassicola 0.006 0.008 0.008 0.617 0.001 Vermicularia knorrii 0.006 0.012 0.000 0.626 0.005 ** Tellina texana 0.006 0.005 0.010 0.634 0.113 Gammarus mucronatus 0.006 0.011 0.002 0.643 0.125 Amphioplus abditus 0.006 0.013 0.004 0.651 1.000 Holothuriidae sp. 0.006 0.005 0.009 0.660 0.002 ** Vitrinella floridana 0.006 0.007 0.007 0.668 0.498 Ophioderma brevispina 0.006 0.008 0.010 0.676 0.448 Pteria colymbus 0.005 0.011 0.000 0.684 0.001 *** Urosalpinx perrugata 0.005 0.009 0.003 0.692 0.206 Hoplomachus propinquus 0.005 0.011 0.001 0.700 0.001 *** Palaemonetes pugio 0.005 0.001 0.010 0.708 0.071 . Ameritella versicolor 0.005 0.000 0.011 0.716 0.197 Bemlos spinicarpus 0.005 0.008 0.004 0.724 0.042 * Vexillum exiguum 0.005 0.010 0.000 0.731 0.001 *** Cyathura polita 0.005 0.007 0.007 0.739 0.999 Granulina hadria 0.005 0.010 0.002 0.747 0.260 Meioceras nitidum 0.005 0.000 0.010 0.754 0.001 *** Phascolion sp. 0.005 0.002 0.010 0.761 0.166 Melanella sp. 0.005 0.000 0.010 0.768 0.367 Astyris lunata 0.005 0.001 0.010 0.776 0.968 Mysella planulata 0.005 0.010 0.007 0.783 0.804 Pinnixa cylindrica 0.005 0.008 0.003 0.790 0.744 Chione elevata 0.005 0.008 0.006 0.797 0.647 Erichsonella attenuata 0.005 0.008 0.006 0.803 0.987 Pyrgospira tampaensis 0.005 0.007 0.003 0.810 0.011 * Polychaete spp. 0.005 0.006 0.005 0.817 0.940 Mitromica foveata 0.005 0.008 0.002 0.824 0.003 ** Pyrgocythara hemphilli 0.005 0.002 0.010 0.830 0.988 Musculus lateralis 0.004 0.001 0.008 0.837 0.006 ** Leptochelia forresti 0.004 0.009 0.000 0.843 0.331 Schwartziella catesbyana 0.004 0.004 0.007 0.849 0.958 Pelia mutica 0.004 0.000 0.008 0.856 0.012 * Nudibranchia sp. A 0.004 0.004 0.007 0.862 0.530 Nucula proxima 0.004 0.001 0.007 0.867 0.015 *

49 Supplementary Materials

Cyclostremiscus suppressus 0.004 0.000 0.007 0.873 0.376 Codakia orbicularis 0.004 0.004 0.008 0.879 0.833 Globosolembos smithi 0.004 0.000 0.007 0.884 0.518 Amphinomidae sp. 0.004 0.004 0.003 0.889 0.707 Isopoda sp. B 0.003 0.007 0.000 0.894 0.006 ** Nassarius vibex 0.003 0.005 0.007 0.899 1.000 Latreutes fucorum 0.003 0.000 0.007 0.904 0.066 . Olivella spp. 0.003 0.002 0.007 0.909 0.999 Bulla occidentalis 0.003 0.009 0.006 0.914 1.000 Paracaprella tenuis 0.003 0.001 0.006 0.919 0.207 Nassarius albus concensus 0.003 0.006 0.000 0.923 0.936 Bittiolum varium 0.003 0.000 0.006 0.928 0.957 Callinectes sapidus 0.003 0.000 0.006 0.932 0.811 Eunicidae sp. A 0.003 0.006 0.003 0.937 0.978 Turbo castanea 0.003 0.006 0.000 0.941 0.001 *** Turbonilla sp. 0.003 0.002 0.004 0.945 0.943 Diopatra sp. 0.003 0.000 0.005 0.949 0.913 Idotea sp. A 0.003 0.005 0.000 0.952 0.517 Rubellatoma diomedea 0.002 0.003 0.003 0.956 0.560 Urosalpinx cinerea 0.002 0.005 0.000 0.960 0.345 Phosinella cancellata complex 0.002 0.005 0.000 0.963 0.001 *** Apocorophium simile 0.002 0.000 0.005 0.967 0.674 Pagurus maclaughlinae 0.002 0.005 0.009 0.970 1.000 Crepidula spp. 0.002 0.001 0.004 0.972 1.000 Lumbrineridae sp. A 0.002 0.004 0.001 0.975 1.000 Cerodrillia spp. 0.002 0.000 0.003 0.978 0.814 Modulus modulus 0.002 0.005 0.005 0.980 1.000 Edotia triloba 0.002 0.000 0.003 0.982 0.984 Urosalpinx tampaensis 0.002 0.000 0.003 0.984 0.761 Erichsonella filiformis floridana 0.002 0.002 0.001 0.987 0.971 Alpheus sp. 0.001 0.000 0.003 0.989 0.924 Acteocina candei 0.001 0.000 0.002 0.990 0.999 Brachidontes exustus 0.001 0.000 0.002 0.992 0.989 Phoxocephalidae sp. 0.001 0.001 0.001 0.993 0.775 Cerithiopsis sp. 0.001 0.000 0.001 0.994 0.990 Terebra protexta 0.001 0.000 0.001 0.995 0.780 Eobrolgus spinosus 0.001 0.000 0.001 0.996 0.993 Harrieta faxoni 0.000 0.000 0.001 0.997 1.000 Pitho spp. 0.000 0.000 0.001 0.997 0.991 Grandidierella bonnieroides 0.000 0.000 0.001 0.998 1.000 Cerithium muscarum 0.000 0.001 0.000 0.999 1.000 Laevicardium mortoni 0.000 0.000 0.001 0.999 1.000 Caecum pulchellum 0.000 0.000 0.001 0.999 1.000 Transennella stimpsoni 0.000 0.000 0.001 1.000 0.958 Transennella conradina 0.000 0.000 0.000 1.000 1.000

50 Supplementary Materials

Boonea impressa 0.000 0.000 0.000 1.000 1.000 Caecum imbricatum 0.000 0.000 0.000 1.000 1.000 Crepidula plana complex 0.000 0.000 0.000 1.000 1.000 Haminoea elegans 0.000 0.000 0.000 1.000 1.000 Lyonsia floridana 0.000 0.000 0.000 1.000 1.000 Mesokalliapseudes macsweenyi 0.000 0.000 0.000 1.000 1.000 Monocorophium acherusicum 0.000 0.000 0.000 1.000 1.000

4.4 Crystal (CRY) vs. Waccasassa (WAC)

Average Average Contribution Abundance Abundance Cumulative CRY vs. WAC % CRY WAC % p Pyrgocythara hemphilli 0.016 0.010 0.040 0.023 0.001 *** Dentimargo eburneolus 0.016 0.023 0.049 0.046 0.004 ** Ischnochiton papillosus 0.015 0.031 0.000 0.069 0.001 *** Elasmopus levis 0.015 0.034 0.007 0.092 0.001 *** Acteocina candei 0.015 0.002 0.032 0.114 0.001 *** Nassarius vibex 0.014 0.007 0.036 0.135 0.001 *** Penaeus duorarum 0.014 0.020 0.025 0.156 0.002 ** Neopanope packardii 0.013 0.037 0.012 0.175 0.001 *** Astyris lunata 0.013 0.010 0.035 0.195 0.001 *** Alpheus packardii 0.013 0.036 0.015 0.214 0.004 ** Libinia dubia 0.013 0.026 0.016 0.233 0.049 * Olivella spp. 0.012 0.007 0.029 0.251 0.004 ** Costoanachis semiplicata 0.012 0.025 0.041 0.268 0.080 . Tozeuma carolinense 0.011 0.014 0.027 0.285 0.017 * Hippolyte zostericola 0.011 0.030 0.020 0.302 0.020 * Prunum apicinum 0.011 0.038 0.038 0.318 0.166 Palaemon floridanus 0.010 0.021 0.003 0.333 0.001 *** Cyathura polita 0.010 0.007 0.021 0.348 0.345 Haminoea elegans 0.010 0.000 0.020 0.363 0.001 *** Pilsbryspira leucocyma 0.010 0.020 0.000 0.378 0.312 Turbonilla sp. 0.009 0.004 0.021 0.392 0.001 *** Lucinisca nassula 0.009 0.019 0.001 0.406 0.009 ** Crepidula spp. 0.009 0.004 0.023 0.420 0.001 *** Melanella sp. 0.009 0.010 0.011 0.433 0.025 * Diopatra sp. 0.009 0.005 0.020 0.447 0.001 *** Haminoea sp. A 0.009 0.013 0.009 0.460 0.388 Conasprella spp. 0.009 0.012 0.012 0.473 0.006 ** Hippolyte pleuracantha 0.009 0.025 0.012 0.486 0.402 Bittiolum varium 0.009 0.006 0.022 0.499 0.002 ** Ampelisca abdita 0.008 0.017 0.005 0.511 0.899 Cymadusa compta 0.008 0.027 0.019 0.522 0.337 Columbella rusticoides 0.008 0.017 0.014 0.534 0.098 .

51 Supplementary Materials

Gammaridea spp. 0.007 0.014 0.004 0.544 0.489 Hippolyte sp. 0.007 0.013 0.005 0.555 0.238 Globosolembos smithi 0.007 0.007 0.011 0.566 0.004 ** Ericthonius punctatus 0.007 0.011 0.005 0.576 0.172 Cyclostremiscus suppressus 0.007 0.007 0.009 0.586 0.002 ** Tellina texana 0.007 0.010 0.007 0.597 0.013 * Eobrolgus spinosus 0.007 0.001 0.014 0.607 0.001 *** Cerithiopsis sp. 0.007 0.001 0.013 0.616 0.013 * Edotia triloba 0.007 0.003 0.011 0.626 0.359 Pagurus maclaughlinae 0.006 0.009 0.021 0.636 0.357 Longchaeus suturalis 0.006 0.013 0.001 0.645 0.008 ** Ophioderma brevispina 0.006 0.010 0.009 0.655 0.178 Phascolion sp. 0.006 0.010 0.013 0.664 0.001 *** Ameritella versicolor 0.006 0.011 0.003 0.673 0.106 Amphioplus abditus 0.006 0.004 0.013 0.682 1.000 Palaemonetes pugio 0.006 0.010 0.003 0.691 0.038 * Lumbrineridae sp. A 0.006 0.001 0.012 0.700 0.894 Cardites floridanus 0.006 0.008 0.010 0.708 0.512 Ascobulla ulla 0.006 0.011 0.001 0.716 0.546 Boonea impressa 0.005 0.000 0.011 0.724 0.004 ** Urosalpinx perrugata 0.005 0.003 0.008 0.732 0.287 Transennella conradina 0.005 0.000 0.011 0.740 0.012 * Eunicidae sp. B 0.005 0.015 0.007 0.747 0.999 Meioceras nitidum 0.005 0.010 0.000 0.755 0.001 *** Echinaster serpentarius 0.005 0.011 0.009 0.762 0.827 Sayella laevigata 0.005 0.009 0.003 0.770 0.533 Ampithoe longimana 0.005 0.005 0.009 0.777 0.950 Thor dobkini 0.005 0.010 0.000 0.784 0.878 Erichsonella attenuata 0.005 0.006 0.009 0.792 0.983 Schwartziella catesbyana 0.005 0.007 0.005 0.799 0.939 Pelia mutica 0.005 0.008 0.003 0.806 0.001 *** Paracaprella tenuis 0.005 0.006 0.005 0.812 0.008 ** Pyrgocythara filosa 0.005 0.009 0.000 0.819 0.979 Holothuriidae sp. 0.005 0.009 0.000 0.826 0.092 . Crepidula plana complex 0.005 0.000 0.009 0.833 0.455 Chione elevata 0.004 0.006 0.008 0.839 0.724 Musculus lateralis 0.004 0.008 0.001 0.846 0.003 ** Latreutes fucorum 0.004 0.007 0.002 0.852 0.012 * Nucula proxima 0.004 0.007 0.002 0.858 0.009 Prunum succineum 0.004 0.008 0.000 0.864 0.683 Paracerceis caudata 0.004 0.006 0.013 0.870 0.999 Vitrinella floridana 0.004 0.007 0.002 0.876 0.827 Mysella planulata 0.004 0.007 0.005 0.882 0.869 Bulla occidentalis 0.004 0.006 0.011 0.888 1.000 Codakia orbicularis 0.004 0.008 0.004 0.894 0.808

52 Supplementary Materials

Alpheus sp. 0.004 0.003 0.006 0.899 0.456 Eulithidium thalassicola 0.004 0.008 0.000 0.905 0.216 Amphinomidae sp. 0.004 0.003 0.004 0.910 0.658 Nudibranchia sp. A 0.003 0.007 0.000 0.915 0.746 Cinctura hunteria 0.003 0.006 0.000 0.920 0.812 Callinectes sapidus 0.003 0.006 0.000 0.924 0.811 Laevicardium mortoni 0.003 0.001 0.006 0.929 0.988 Apocorophium simile 0.003 0.005 0.001 0.933 0.532 Polychaete spp. 0.003 0.005 0.004 0.937 0.988 Urosalpinx cinerea 0.003 0.000 0.005 0.941 0.313 Granulina hadria 0.002 0.002 0.004 0.945 0.941 Bemlos spinicarpus 0.002 0.004 0.001 0.948 0.653 Pyrgospira tampaensis 0.002 0.003 0.002 0.952 0.501 Alpheus heterochaelis 0.002 0.003 0.003 0.955 1.000 Eunicidae sp. A 0.002 0.003 0.003 0.958 0.999 Leptochelia rapax 0.002 0.003 0.002 0.961 1.000 Pinnixa cylindrica 0.002 0.003 0.001 0.964 0.998 Lysianopsis alba 0.002 0.004 0.000 0.967 1.000 Modulus modulus 0.002 0.005 0.008 0.970 1.000 Caecum pulchellum 0.002 0.001 0.004 0.973 0.990 Dulichiella appendiculata 0.002 0.003 0.000 0.975 0.908 Cerodrillia spp. 0.002 0.003 0.000 0.978 0.809 Urosalpinx tampaensis 0.002 0.003 0.000 0.980 0.796 Rubellatoma diomedea 0.001 0.003 0.000 0.982 0.760 Cerithium muscarum 0.001 0.000 0.003 0.984 1.000 Batea cuspidata 0.001 0.003 0.000 0.986 1.000 Mitromica foveata 0.001 0.002 0.000 0.988 0.719 Phoxocephalidae sp. 0.001 0.001 0.001 0.989 0.724 Brachidontes exustus 0.001 0.002 0.001 0.991 0.993 Gammarus mucronatus 0.001 0.002 0.000 0.992 0.987 Vitreolina conica 0.001 0.001 0.001 0.993 0.992 Harrieta faxoni 0.001 0.001 0.001 0.995 1.000 Terebra protexta 0.001 0.001 0.000 0.996 0.787 Transennella stimpsoni 0.001 0.001 0.001 0.997 0.853 Erichsonella filiformis floridana 0.001 0.001 0.000 0.997 0.999 Pitho spp. 0.000 0.001 0.000 0.998 0.996 Monocorophium acherusicum 0.000 0.000 0.001 0.999 0.999 Grandidierella bonnieroides 0.000 0.001 0.000 0.999 1.000 Hoplomachus propinquus 0.000 0.001 0.000 1.000 0.991 Nassarius albus concensus 0.000 0.000 0.000 1.000 1.000 Caecum imbricatum 0.000 0.000 0.000 1.000 1.000 Cerithium atratum 0.000 0.000 0.000 1.000 1.000 Idotea sp. A 0.000 0.000 0.000 1.000 1.000 Isopoda sp. B 0.000 0.000 0.000 1.000 1.000 Leptochelia forresti 0.000 0.000 0.000 1.000 1.000

53 Supplementary Materials

Lyonsia floridana 0.000 0.000 0.000 1.000 1.000 Mesokalliapseudes macsweenyi 0.000 0.000 0.000 1.000 1.000 Phosinella cancellata complex 0.000 0.000 0.000 1.000 1.000 Pteria colymbus 0.000 0.000 0.000 1.000 1.000 Pyrgocythara plicosa 0.000 0.000 0.000 1.000 1.000 Turbo castanea 0.000 0.000 0.000 1.000 1.000 Vermicularia knorrii 0.000 0.000 0.000 1.000 1.000 Vexillum exiguum 0.000 0.000 0.000 1.000 1.000

4.5 Weeki Wachee (WEE) vs. Homosassa (HOM)

Average Average Contribution Abundance Abundance Cumulative WEE vs. HOM % WEE HOM % p Caecum pulchellum 0.019 0.038 0.000 0.025 0.001 *** Ischnochiton papillosus 0.017 0.002 0.036 0.047 0.001 *** Laevicardium mortoni 0.017 0.033 0.000 0.070 0.001 *** Paracerceis caudata 0.016 0.002 0.033 0.091 0.001 *** Nassarius vibex 0.016 0.035 0.005 0.111 0.001 *** Batea cuspidata 0.015 0.002 0.032 0.132 0.001 *** Amphioplus abditus 0.015 0.033 0.013 0.152 0.038 * Bulla occidentalis 0.015 0.035 0.009 0.172 0.003 Polychaete spp. 0.014 0.029 0.006 0.191 0.004 ** Schwartziella catesbyana 0.012 0.027 0.004 0.208 0.002 ** Grandidierella bonnieroides 0.012 0.025 0.000 0.224 0.001 *** Leptochelia rapax 0.012 0.002 0.026 0.240 0.001 *** Alpheus heterochaelis 0.012 0.013 0.031 0.257 0.297 Lysianopsis alba 0.012 0.014 0.030 0.273 0.001 *** Cerithium atratum 0.012 0.015 0.016 0.289 0.013 * Pagurus maclaughlinae 0.012 0.029 0.005 0.305 0.001 *** Nassarius albus concensus 0.012 0.026 0.006 0.320 0.001 *** Echinaster serpentarius 0.011 0.000 0.023 0.335 0.001 *** Pyrgocythara plicosa 0.011 0.000 0.022 0.350 0.001 *** Erichsonella attenuata 0.010 0.019 0.008 0.364 0.164 Hippolyte zostericola 0.010 0.007 0.028 0.377 0.072 . Cardites floridanus 0.010 0.000 0.020 0.391 0.001 *** Ampithoe longimana 0.010 0.014 0.017 0.404 0.006 ** Hippolyte pleuracantha 0.010 0.013 0.030 0.417 0.079 . Eunicidae sp. A 0.009 0.022 0.006 0.430 0.039 * Ampelisca abdita 0.009 0.022 0.009 0.442 0.608 Brachidontes exustus 0.009 0.018 0.000 0.454 0.001 *** Pyrgocythara filosa 0.008 0.004 0.018 0.465 0.361 Pilsbryspira leucocyma 0.008 0.019 0.019 0.476 0.729 Thor dobkini 0.008 0.010 0.014 0.487 0.238 Haminoea sp. A 0.008 0.000 0.016 0.497 0.507

54 Supplementary Materials

Libinia dubia 0.008 0.013 0.006 0.508 0.935 Cyathura polita 0.008 0.018 0.007 0.519 0.817 Tozeuma carolinense 0.008 0.013 0.016 0.529 0.917 Cymadusa compta 0.008 0.018 0.027 0.539 0.301 Vitrinella floridana 0.008 0.012 0.007 0.550 0.144 Ascobulla ulla 0.008 0.009 0.013 0.560 0.097 . Codakia orbicularis 0.007 0.014 0.004 0.569 0.151 Edotia triloba 0.007 0.014 0.000 0.579 0.244 Pinnixa cylindrica 0.007 0.013 0.008 0.588 0.224 Sayella laevigata 0.007 0.006 0.014 0.597 0.029 * Dentimargo eburneolus 0.007 0.008 0.017 0.606 0.996 Eunicidae sp. B 0.007 0.025 0.024 0.615 0.793 Vitreolina conica 0.007 0.003 0.013 0.624 0.001 *** Dulichiella appendiculata 0.007 0.000 0.013 0.633 0.001 *** Amphinomidae sp. 0.006 0.010 0.004 0.641 0.124 Cinctura hunteria 0.006 0.005 0.010 0.650 0.173 Gammaridea spp. 0.006 0.010 0.006 0.658 0.782 Vermicularia knorrii 0.006 0.000 0.012 0.666 0.003 ** Lumbrineridae sp. A 0.006 0.012 0.004 0.674 0.862 Penaeus duorarum 0.006 0.006 0.010 0.682 0.990 Transennella stimpsoni 0.006 0.012 0.000 0.689 0.009 ** Gammarus mucronatus 0.006 0.000 0.011 0.697 0.116 Monocorophium acherusicum 0.006 0.011 0.000 0.704 0.230 Ophioderma brevispina 0.006 0.007 0.008 0.712 0.379 Lyonsia floridana 0.006 0.011 0.000 0.720 0.045 * Pteria colymbus 0.005 0.000 0.011 0.727 0.002 ** Mesokalliapseudes macsweenyi 0.005 0.011 0.000 0.734 0.086 . Hoplomachus propinquus 0.005 0.000 0.011 0.741 0.001 *** Leptochelia forresti 0.005 0.003 0.009 0.748 0.189 Idotea sp. A 0.005 0.007 0.005 0.756 0.025 * Hippolyte sp. 0.005 0.002 0.011 0.763 0.651 Cerithium muscarum 0.005 0.011 0.001 0.770 0.761 Prunum apicinum 0.005 0.015 0.021 0.777 1.000 Vexillum exiguum 0.005 0.000 0.010 0.783 0.001 *** Columbella rusticoides 0.005 0.002 0.009 0.790 0.813 Prunum succineum 0.005 0.000 0.010 0.797 0.345 Granulina hadria 0.005 0.001 0.010 0.804 0.273 Harrieta faxoni 0.005 0.010 0.000 0.810 0.141 Palaemon floridanus 0.005 0.001 0.010 0.817 0.787 Caecum imbricatum 0.005 0.010 0.000 0.823 0.001 *** Pitho spp. 0.005 0.009 0.000 0.829 0.003 ** Transennella conradina 0.005 0.009 0.000 0.836 0.114 Mysella planulata 0.005 0.001 0.010 0.842 0.826 Urosalpinx perrugata 0.004 0.000 0.009 0.848 0.368 Neopanope packardii 0.004 0.019 0.013 0.853 0.982

55 Supplementary Materials

Bemlos spinicarpus 0.004 0.000 0.008 0.859 0.236 Chione elevata 0.004 0.001 0.008 0.864 0.888 Eulithidium thalassicola 0.004 0.000 0.008 0.869 0.167 Ericthonius punctatus 0.004 0.004 0.005 0.875 0.712 Mitromica foveata 0.004 0.000 0.008 0.880 0.027 * Elasmopus levis 0.004 0.008 0.009 0.885 0.975 Modulus modulus 0.004 0.009 0.005 0.890 0.973 Alpheus packardii 0.004 0.008 0.003 0.894 1.000 Isopoda sp. B 0.003 0.000 0.007 0.899 0.002 ** Pyrgospira tampaensis 0.003 0.000 0.007 0.904 0.151 Crepidula spp. 0.003 0.008 0.001 0.908 0.987 Ameritella versicolor 0.003 0.006 0.000 0.912 0.621 Crepidula plana complex 0.003 0.006 0.000 0.917 0.630 Cerithiopsis sp. 0.003 0.006 0.000 0.921 0.674 Bittiolum varium 0.003 0.006 0.000 0.925 0.946 Olivella spp. 0.003 0.006 0.002 0.929 1.000 Cerodrillia spp. 0.003 0.006 0.000 0.933 0.453 Erichsonella filiformis floridana 0.003 0.005 0.002 0.936 0.797 Callinectes sapidus 0.003 0.006 0.000 0.940 0.944 Tellina texana 0.003 0.002 0.005 0.944 0.812 Turbo castanea 0.003 0.000 0.006 0.948 0.001 *** Costoanachis semiplicata 0.003 0.004 0.005 0.951 1.000 Terebra protexta 0.003 0.005 0.000 0.955 0.244 Astyris lunata 0.003 0.006 0.001 0.958 1.000 Lucinisca nassula 0.003 0.000 0.005 0.961 0.992 Urosalpinx cinerea 0.002 0.000 0.005 0.965 0.357 Phosinella cancellata complex 0.002 0.000 0.005 0.968 0.001 *** Alpheus sp. 0.002 0.005 0.000 0.971 0.777 Holothuriidae sp. 0.002 0.000 0.005 0.974 0.513 Urosalpinx tampaensis 0.002 0.005 0.000 0.977 0.624 Palaemonetes pugio 0.002 0.003 0.001 0.980 0.768 Pyrgocythara hemphilli 0.002 0.004 0.002 0.983 1.000 Nudibranchia sp. A 0.002 0.000 0.004 0.986 0.954 Rubellatoma diomedea 0.002 0.001 0.003 0.988 0.723 Conasprella spp. 0.002 0.001 0.003 0.990 0.977 Globosolembos smithi 0.001 0.002 0.000 0.992 0.979 Turbonilla sp. 0.001 0.001 0.002 0.993 1.000 Diopatra sp. 0.001 0.002 0.000 0.994 1.000 Phascolion sp. 0.001 0.002 0.002 0.995 1.000 Apocorophium simile 0.001 0.001 0.000 0.996 0.886 Nucula proxima 0.000 0.000 0.001 0.997 0.889 Cyclostremiscus suppressus 0.000 0.000 0.000 0.997 0.978 Pelia mutica 0.000 0.001 0.000 0.998 0.980 Musculus lateralis 0.000 0.000 0.001 0.998 0.953 Paracaprella tenuis 0.000 0.000 0.001 0.999 0.956

56 Supplementary Materials

Phoxocephalidae sp. 0.000 0.000 0.001 0.999 0.958 Acteocina candei 0.000 0.001 0.000 1.000 1.000 Meioceras nitidum 0.000 0.000 0.000 1.000 0.990 Boonea impressa 0.000 0.000 0.000 1.000 1.000 Eobrolgus spinosus 0.000 0.000 0.000 1.000 1.000 Haminoea elegans 0.000 0.000 0.000 1.000 1.000 Latreutes fucorum 0.000 0.000 0.000 1.000 1.000 Longchaeus suturalis 0.000 0.000 0.000 1.000 1.000 Melanella sp. 0.000 0.000 0.000 1.000 1.000

4.6 Homosassa (HOM) vs. Waccasassa (WAC)

Average Average Contribution Abundance Abundance Cumulative HOM vs. WAC % HOM WAC % p Pyrgocythara hemphilli 0.019 0.002 0.040 0.026 0.001 *** Costoanachis semiplicata 0.018 0.005 0.041 0.050 0.001 *** Ischnochiton papillosus 0.018 0.036 0.000 0.073 0.001 *** Dentimargo eburneolus 0.017 0.017 0.049 0.097 0.001 *** Astyris lunata 0.017 0.001 0.035 0.120 0.001 *** Batea cuspidata 0.016 0.032 0.000 0.141 0.001 *** Acteocina candei 0.016 0.000 0.032 0.163 0.001 *** Nassarius vibex 0.016 0.005 0.036 0.184 0.001 *** Lysianopsis alba 0.015 0.030 0.000 0.204 0.001 *** Alpheus heterochaelis 0.014 0.031 0.003 0.223 0.032 * Olivella spp. 0.014 0.002 0.029 0.241 0.001 *** Leptochelia rapax 0.012 0.026 0.002 0.257 0.001 *** Penaeus duorarum 0.012 0.010 0.025 0.273 0.088 . Paracerceis caudata 0.011 0.033 0.013 0.288 0.013 * Pyrgocythara plicosa 0.011 0.022 0.000 0.303 0.001 *** Bittiolum varium 0.011 0.000 0.022 0.318 0.001 *** Crepidula spp. 0.011 0.001 0.023 0.332 0.001 *** Tozeuma carolinense 0.010 0.016 0.027 0.346 0.189 Diopatra sp. 0.010 0.000 0.020 0.359 0.001 *** Hippolyte pleuracantha 0.010 0.030 0.012 0.373 0.051 . Haminoea elegans 0.010 0.000 0.020 0.386 0.001 *** Haminoea sp. A 0.010 0.016 0.009 0.399 0.208 Turbonilla sp. 0.010 0.002 0.021 0.412 0.001 *** Cyathura polita 0.010 0.007 0.021 0.425 0.423 Echinaster serpentarius 0.010 0.023 0.009 0.438 0.001 *** Pilsbryspira leucocyma 0.009 0.019 0.000 0.451 0.419 Prunum apicinum 0.009 0.021 0.038 0.463 0.600 Libinia dubia 0.009 0.006 0.016 0.476 0.786 Pyrgocythara filosa 0.009 0.018 0.000 0.488 0.250 Eunicidae sp. B 0.009 0.024 0.007 0.499 0.017 *

57 Supplementary Materials

Cardites floridanus 0.009 0.020 0.010 0.511 0.001 *** Ampithoe longimana 0.008 0.017 0.009 0.522 0.198 Pagurus maclaughlinae 0.008 0.005 0.021 0.533 0.004 Cerithium atratum 0.008 0.016 0.000 0.544 0.316 Hippolyte zostericola 0.008 0.028 0.020 0.554 0.762 Urosalpinx perrugata 0.007 0.009 0.008 0.564 0.044 * Sayella laevigata 0.007 0.014 0.003 0.573 0.020 * Columbella rusticoides 0.007 0.009 0.014 0.583 0.284 Eobrolgus spinosus 0.007 0.000 0.014 0.592 0.001 *** Thor dobkini 0.007 0.014 0.000 0.601 0.547 Cymadusa compta 0.007 0.027 0.019 0.610 0.725 Dulichiella appendiculata 0.007 0.013 0.000 0.619 0.001 *** Vitreolina conica 0.007 0.013 0.001 0.628 0.001 *** Amphioplus abditus 0.007 0.013 0.013 0.636 1.000 Cerithiopsis sp. 0.007 0.000 0.013 0.645 0.013 * Ascobulla ulla 0.007 0.013 0.001 0.654 0.298 Alpheus packardii 0.006 0.003 0.015 0.662 0.866 Conasprella spp. 0.006 0.003 0.012 0.671 0.199 Vermicularia knorrii 0.006 0.012 0.000 0.679 0.002 ** Ophioderma brevispina 0.006 0.008 0.009 0.687 0.272 Granulina hadria 0.006 0.010 0.004 0.695 0.112 Gammarus mucronatus 0.006 0.011 0.000 0.702 0.117 Edotia triloba 0.006 0.000 0.011 0.710 0.512 Globosolembos smithi 0.006 0.000 0.011 0.717 0.080 . Pteria colymbus 0.005 0.011 0.000 0.725 0.001 *** Melanella sp. 0.005 0.000 0.011 0.732 0.355 Hippolyte sp. 0.005 0.011 0.005 0.739 0.645 Hoplomachus propinquus 0.005 0.011 0.000 0.746 0.001 *** Phascolion sp. 0.005 0.002 0.013 0.754 0.071 . Lumbrineridae sp. A 0.005 0.004 0.012 0.761 0.930 Transennella conradina 0.005 0.000 0.011 0.768 0.007 ** Boonea impressa 0.005 0.000 0.011 0.775 0.003 ** Vexillum exiguum 0.005 0.010 0.000 0.782 0.001 *** Prunum succineum 0.005 0.010 0.000 0.789 0.362 Palaemon floridanus 0.005 0.010 0.003 0.795 0.803 Cinctura hunteria 0.005 0.010 0.000 0.802 0.442 Ampelisca abdita 0.005 0.009 0.005 0.809 1.000 Cyclostremiscus suppressus 0.005 0.000 0.009 0.815 0.150 Tellina texana 0.005 0.005 0.007 0.822 0.315 Bemlos spinicarpus 0.005 0.008 0.001 0.828 0.183 Crepidula plana complex 0.005 0.000 0.009 0.834 0.479 Mysella planulata 0.004 0.010 0.005 0.840 0.833 Urosalpinx cinerea 0.004 0.005 0.005 0.846 0.006 ** Erichsonella attenuata 0.004 0.008 0.009 0.851 0.996 Leptochelia forresti 0.004 0.009 0.000 0.857 0.333

58 Supplementary Materials

Ericthonius punctatus 0.004 0.005 0.005 0.863 0.641 Elasmopus levis 0.004 0.009 0.007 0.868 0.949 Polychaete spp. 0.004 0.006 0.004 0.874 0.966 Bulla occidentalis 0.004 0.009 0.011 0.879 1.000 Eulithidium thalassicola 0.004 0.008 0.000 0.884 0.179 Pyrgospira tampaensis 0.004 0.007 0.002 0.889 0.094 . Mitromica foveata 0.004 0.008 0.000 0.894 0.033 * Amphinomidae sp. 0.004 0.004 0.004 0.900 0.629 Gammaridea spp. 0.004 0.006 0.004 0.904 0.986 Chione elevata 0.004 0.008 0.008 0.909 0.951 Vitrinella floridana 0.004 0.007 0.002 0.914 0.855 Pinnixa cylindrica 0.004 0.008 0.001 0.919 0.929 Isopoda sp. B 0.003 0.007 0.000 0.924 0.003 ** Schwartziella catesbyana 0.003 0.004 0.005 0.928 0.993 Nassarius albus concensus 0.003 0.006 0.000 0.932 0.941 Laevicardium mortoni 0.003 0.000 0.006 0.936 0.989 Alpheus sp. 0.003 0.000 0.006 0.940 0.663 Turbo castanea 0.003 0.006 0.000 0.944 0.001 *** Neopanope packardii 0.003 0.013 0.012 0.947 1.000 Lucinisca nassula 0.003 0.005 0.001 0.951 0.996 Paracaprella tenuis 0.003 0.001 0.005 0.954 0.362 Idotea sp. A 0.003 0.005 0.000 0.958 0.519 Eunicidae sp. A 0.002 0.006 0.003 0.961 0.993 Phosinella cancellata complex 0.002 0.005 0.000 0.964 0.001 *** Holothuriidae sp. 0.002 0.005 0.000 0.967 0.525 Codakia orbicularis 0.002 0.004 0.004 0.970 0.999 Nudibranchia sp. A 0.002 0.004 0.000 0.973 0.930 Modulus modulus 0.002 0.005 0.008 0.976 1.000 Palaemonetes pugio 0.002 0.001 0.003 0.978 0.780 Caecum pulchellum 0.002 0.000 0.004 0.981 0.996 Ameritella versicolor 0.002 0.000 0.003 0.983 0.888 Pelia mutica 0.001 0.000 0.003 0.985 0.755 Rubellatoma diomedea 0.001 0.003 0.000 0.987 0.852 Cerithium muscarum 0.001 0.001 0.003 0.988 1.000 Latreutes fucorum 0.001 0.000 0.002 0.990 0.812 Erichsonella filiformis floridana 0.001 0.002 0.000 0.991 0.988 Nucula proxima 0.001 0.001 0.002 0.993 0.736 Musculus lateralis 0.001 0.001 0.001 0.994 0.849 Phoxocephalidae sp. 0.001 0.001 0.001 0.995 0.809 Harrieta faxoni 0.001 0.000 0.001 0.996 1.000 Longchaeus suturalis 0.001 0.000 0.001 0.997 1.000 Apocorophium simile 0.001 0.000 0.001 0.998 0.941 Brachidontes exustus 0.000 0.000 0.001 0.999 1.000 Monocorophium acherusicum 0.000 0.000 0.001 0.999 0.997 Transennella stimpsoni 0.000 0.000 0.001 1.000 0.924

59 Supplementary Materials

Meioceras nitidum 0.000 0.000 0.000 1.000 0.996 Caecum imbricatum 0.000 0.000 0.000 1.000 1.000 Callinectes sapidus 0.000 0.000 0.000 1.000 1.000 Cerodrillia spp. 0.000 0.000 0.000 1.000 1.000 Grandidierella bonnieroides 0.000 0.000 0.000 1.000 1.000 Lyonsia floridana 0.000 0.000 0.000 1.000 1.000 Mesokalliapseudes macsweenyi 0.000 0.000 0.000 1.000 1.000 Pitho spp. 0.000 0.000 0.000 1.000 1.000 Terebra protexta 0.000 0.000 0.000 1.000 1.000 Urosalpinx tampaensis 0.000 0.000 0.000 1.000 1.000

4.7 Weeki Wachee (WEE) vs. Waccasassa (WAC)

Average Average Contribution Abundance Abundance Cumulative WEE vs. WAC % WEE WAC % p Dentimargo eburneolus 0.021 0.008 0.049 0.029 0.001 *** Costoanachis semiplicata 0.018 0.004 0.041 0.054 0.001 *** Pyrgocythara hemphilli 0.018 0.004 0.040 0.079 0.001 *** Caecum pulchellum 0.018 0.038 0.004 0.103 0.001 *** Acteocina candei 0.016 0.001 0.032 0.125 0.001 *** Amphioplus abditus 0.015 0.033 0.013 0.145 0.028 * Astyris lunata 0.015 0.006 0.035 0.166 0.001 *** Bulla occidentalis 0.015 0.035 0.011 0.186 0.005 ** Polychaete spp. 0.014 0.029 0.004 0.205 0.007 ** Laevicardium mortoni 0.014 0.033 0.006 0.224 0.001 *** Nassarius albus concensus 0.013 0.026 0.000 0.241 0.001 *** Schwartziella catesbyana 0.013 0.027 0.005 0.259 0.001 *** Grandidierella bonnieroides 0.012 0.025 0.000 0.275 0.001 *** Penaeus duorarum 0.012 0.006 0.025 0.292 0.037 * Olivella spp. 0.012 0.006 0.029 0.309 0.001 Prunum apicinum 0.012 0.015 0.038 0.325 0.040 * Libinia dubia 0.011 0.013 0.016 0.340 0.427 Nassarius vibex 0.010 0.035 0.036 0.354 0.456 Erichsonella attenuata 0.010 0.019 0.009 0.369 0.180 Turbonilla sp. 0.010 0.001 0.021 0.383 0.001 *** Eunicidae sp. A 0.010 0.022 0.003 0.397 0.010 ** Edotia triloba 0.010 0.014 0.011 0.410 0.007 ** Tozeuma carolinense 0.010 0.013 0.027 0.424 0.295 Bittiolum varium 0.010 0.006 0.022 0.438 0.001 *** Cyathura polita 0.010 0.018 0.021 0.451 0.383 Haminoea elegans 0.010 0.000 0.020 0.465 0.001 *** Diopatra sp. 0.010 0.002 0.020 0.478 0.001 *** Eunicidae sp. B 0.010 0.025 0.007 0.491 0.002 ** Ampelisca abdita 0.009 0.022 0.005 0.504 0.598

60 Supplementary Materials

Pilsbryspira leucocyma 0.009 0.019 0.000 0.517 0.461 Brachidontes exustus 0.009 0.018 0.001 0.528 0.001 *** Ampithoe longimana 0.009 0.014 0.009 0.540 0.160 Crepidula spp. 0.008 0.008 0.023 0.551 0.019 * Cerithiopsis sp. 0.008 0.006 0.013 0.561 0.001 *** Cerithium atratum 0.008 0.015 0.000 0.572 0.380 Codakia orbicularis 0.007 0.014 0.004 0.582 0.151 Hippolyte zostericola 0.007 0.007 0.020 0.592 0.820 Lumbrineridae sp. A 0.007 0.012 0.012 0.602 0.657 Crepidula plana complex 0.007 0.006 0.009 0.611 0.151 Columbella rusticoides 0.007 0.002 0.014 0.621 0.275 Eobrolgus spinosus 0.007 0.000 0.014 0.630 0.001 *** Lysianopsis alba 0.007 0.014 0.000 0.639 0.716 Pagurus maclaughlinae 0.007 0.029 0.021 0.649 0.273 Pinnixa cylindrica 0.006 0.013 0.001 0.658 0.313 Vitrinella floridana 0.006 0.012 0.002 0.666 0.314 Amphinomidae sp. 0.006 0.010 0.004 0.675 0.117 Ophioderma brevispina 0.006 0.007 0.009 0.684 0.194 Paracerceis caudata 0.006 0.002 0.013 0.692 0.906 Globosolembos smithi 0.006 0.002 0.011 0.700 0.035 * Transennella stimpsoni 0.006 0.012 0.001 0.708 0.002 ** Monocorophium acherusicum 0.006 0.011 0.001 0.717 0.212 Gammaridea spp. 0.006 0.010 0.004 0.725 0.781 Conasprella spp. 0.006 0.001 0.012 0.733 0.276 Transennella conradina 0.006 0.009 0.011 0.740 0.003 ** Lyonsia floridana 0.006 0.011 0.000 0.748 0.044 * Alpheus heterochaelis 0.006 0.013 0.003 0.756 0.999 Melanella sp. 0.005 0.000 0.011 0.763 0.332 Mesokalliapseudes macsweenyi 0.005 0.011 0.000 0.771 0.081 . Hippolyte pleuracantha 0.005 0.013 0.012 0.778 1.000 Phascolion sp. 0.005 0.002 0.013 0.786 0.058 Boonea impressa 0.005 0.000 0.011 0.793 0.003 ** Alpheus packardii 0.005 0.008 0.015 0.800 0.958 Cardites floridanus 0.005 0.000 0.010 0.807 0.632 Cymadusa compta 0.005 0.018 0.019 0.814 0.990 Thor dobkini 0.005 0.010 0.000 0.821 0.863 Cyclostremiscus suppressus 0.005 0.000 0.009 0.828 0.164 Caecum imbricatum 0.005 0.010 0.000 0.834 0.001 *** Harrieta faxoni 0.005 0.010 0.001 0.841 0.194 Haminoea sp. A 0.005 0.000 0.009 0.847 0.934 Pitho spp. 0.005 0.009 0.000 0.854 0.004 ** Cerithium muscarum 0.005 0.011 0.003 0.860 0.875 Alpheus sp. 0.005 0.005 0.006 0.866 0.382 Ascobulla ulla 0.004 0.009 0.001 0.872 0.833 Neopanope packardii 0.004 0.019 0.012 0.878 0.985

61 Supplementary Materials

Ameritella versicolor 0.004 0.006 0.003 0.884 0.376 Echinaster serpentarius 0.004 0.000 0.009 0.890 0.931 Urosalpinx perrugata 0.004 0.000 0.008 0.896 0.428 Sayella laevigata 0.004 0.006 0.003 0.901 0.838 Tellina texana 0.004 0.002 0.007 0.907 0.525 Chione elevata 0.004 0.001 0.008 0.912 0.915 Ericthonius punctatus 0.004 0.004 0.005 0.917 0.733 Modulus modulus 0.004 0.009 0.008 0.922 0.974 Elasmopus levis 0.004 0.008 0.007 0.927 0.988 Idotea sp. A 0.003 0.007 0.000 0.932 0.268 Cerodrillia spp. 0.003 0.006 0.000 0.936 0.473 Callinectes sapidus 0.003 0.006 0.000 0.940 0.931 Hippolyte sp. 0.003 0.002 0.005 0.944 0.987 Palaemonetes pugio 0.003 0.003 0.003 0.947 0.644 Mysella planulata 0.003 0.001 0.005 0.951 0.991 Urosalpinx cinerea 0.003 0.000 0.005 0.954 0.342 Terebra protexta 0.003 0.005 0.000 0.958 0.260 Cinctura hunteria 0.002 0.005 0.000 0.961 0.896 Paracaprella tenuis 0.002 0.000 0.005 0.964 0.398 Granulina hadria 0.002 0.001 0.004 0.968 0.946 Urosalpinx tampaensis 0.002 0.005 0.000 0.971 0.640 Erichsonella filiformis floridana 0.002 0.005 0.000 0.974 0.889 Pyrgocythara filosa 0.002 0.004 0.000 0.976 1.000 Pelia mutica 0.002 0.001 0.003 0.979 0.717 Vitreolina conica 0.002 0.003 0.001 0.981 0.916 Leptochelia rapax 0.002 0.002 0.002 0.983 1.000 Palaemon floridanus 0.001 0.001 0.003 0.985 1.000 Leptochelia forresti 0.001 0.003 0.000 0.987 0.829 Latreutes fucorum 0.001 0.000 0.002 0.989 0.811 Ischnochiton papillosus 0.001 0.002 0.000 0.990 1.000 Apocorophium simile 0.001 0.001 0.001 0.992 0.837 Batea cuspidata 0.001 0.002 0.000 0.993 1.000 Pyrgospira tampaensis 0.001 0.000 0.002 0.994 0.933 Nucula proxima 0.001 0.000 0.002 0.995 0.831 Longchaeus suturalis 0.001 0.000 0.001 0.996 1.000 Rubellatoma diomedea 0.001 0.001 0.000 0.997 0.928 Musculus lateralis 0.001 0.000 0.001 0.998 0.897 Bemlos spinicarpus 0.001 0.000 0.001 0.998 0.983 Phoxocephalidae sp. 0.001 0.000 0.001 0.999 0.928 Lucinisca nassula 0.000 0.000 0.001 1.000 1.000 Gammarus mucronatus 0.000 0.000 0.000 1.000 1.000 Meioceras nitidum 0.000 0.000 0.000 1.000 0.999 Phosinella cancellata complex 0.000 0.000 0.000 1.000 0.988 Dulichiella appendiculata 0.000 0.000 0.000 1.000 1.000 Eulithidium thalassicola 0.000 0.000 0.000 1.000 1.000

62 Supplementary Materials

Holothuriidae sp. 0.000 0.000 0.000 1.000 1.000 Hoplomachus propinquus 0.000 0.000 0.000 1.000 1.000 Isopoda sp. B 0.000 0.000 0.000 1.000 1.000 Mitromica foveata 0.000 0.000 0.000 1.000 1.000 Nudibranchia sp. A 0.000 0.000 0.000 1.000 1.000 Prunum succineum 0.000 0.000 0.000 1.000 1.000 Pteria colymbus 0.000 0.000 0.000 1.000 1.000 Pyrgocythara plicosa 0.000 0.000 0.000 1.000 1.000 Turbo castanea 0.000 0.000 0.000 1.000 1.000 Vermicularia knorrii 0.000 0.000 0.000 1.000 1.000 Vexillum exiguum 0.000 0.000 0.000 1.000 1.000

4.8 Low vs. High Aboveground Biomass Categories

Average Average Low vs. High Aboveground Contribution Abundance Abundance Cumulative Biomass % Low High % p Amphioplus abditus 0.014 0.033 0.009 0.020 0.001 *** Bulla occidentalis 0.012 0.028 0.010 0.037 0.001 *** Dentimargo eburneolus 0.012 0.017 0.024 0.054 0.005 ** Ischnochiton papillosus 0.012 0.008 0.026 0.070 0.001 *** Alpheus heterochaelis 0.011 0.020 0.017 0.086 0.129 Nassarius vibex 0.011 0.023 0.012 0.101 0.024 * Costoanachis semiplicata 0.010 0.013 0.020 0.115 0.040 * Libinia dubia 0.010 0.015 0.015 0.130 0.822 Ampelisca abdita 0.010 0.023 0.012 0.144 0.007 ** Penaeus duorarum 0.010 0.009 0.019 0.158 0.028 * Prunum apicinum 0.010 0.022 0.032 0.172 0.013 * Cyathura polita 0.010 0.018 0.013 0.186 0.099 . Tozeuma carolinense 0.009 0.016 0.016 0.199 0.757 Hippolyte zostericola 0.009 0.014 0.026 0.211 0.003 ** Pilsbryspira leucocyma 0.009 0.016 0.017 0.224 0.851 Erichsonella attenuata 0.009 0.017 0.008 0.237 0.038 * Batea cuspidata 0.009 0.009 0.014 0.249 0.358 Polychaete spp. 0.009 0.015 0.006 0.261 0.092 . Alpheus packardii 0.009 0.007 0.018 0.273 0.019 * Hippolyte pleuracantha 0.009 0.016 0.024 0.285 0.015 * Haminoea sp. A 0.008 0.005 0.015 0.297 0.011 * Lumbrineridae sp. A 0.008 0.018 0.005 0.309 0.005 ** Paracerceis caudata 0.008 0.010 0.018 0.321 0.025 * Pyrgocythara hemphilli 0.008 0.011 0.013 0.332 0.458 Caecum pulchellum 0.008 0.016 0.001 0.344 0.002 ** Cerithium muscarum 0.008 0.016 0.001 0.355 0.001 *** Modulus modulus 0.008 0.019 0.006 0.366 0.001 *** Pyrgocythara filosa 0.008 0.012 0.009 0.377 0.828

63 Supplementary Materials

Neopanope packardii 0.008 0.013 0.022 0.388 0.021 * Olivella spp. 0.008 0.014 0.009 0.398 0.524 Echinaster serpentarius 0.008 0.002 0.016 0.409 0.001 *** Elasmopus levis 0.008 0.009 0.016 0.420 0.047 * Lysianopsis alba 0.008 0.010 0.014 0.430 0.499 Schwartziella catesbyana 0.007 0.014 0.006 0.441 0.043 * Gammaridea spp. 0.007 0.010 0.009 0.451 0.634 Astyris lunata 0.007 0.008 0.013 0.461 0.136 Eunicidae sp. B 0.007 0.019 0.017 0.471 0.935 Cymadusa compta 0.007 0.020 0.025 0.481 0.310 Pagurus maclaughlinae 0.007 0.022 0.010 0.491 0.001 *** Laevicardium mortoni 0.007 0.014 0.002 0.501 0.001 *** Ampithoe longimana 0.007 0.010 0.010 0.511 0.756 Thor dobkini 0.007 0.009 0.009 0.521 0.787 Cerithium atratum 0.007 0.010 0.006 0.530 0.732 Palaemon floridanus 0.007 0.004 0.014 0.540 0.002 Mysella planulata 0.007 0.008 0.010 0.549 0.297 Eunicidae sp. A 0.007 0.014 0.005 0.558 0.008 ** Columbella rusticoides 0.007 0.005 0.012 0.567 0.010 ** Cardites floridanus 0.006 0.003 0.014 0.576 0.001 *** Leptochelia rapax 0.006 0.005 0.012 0.585 0.033 * Hippolyte sp. 0.006 0.006 0.009 0.594 0.455 Lucinisca nassula 0.006 0.007 0.008 0.602 0.488 Acteocina candei 0.006 0.007 0.007 0.610 0.641 Ascobulla ulla 0.006 0.004 0.010 0.618 0.135 Callinectes sapidus 0.006 0.010 0.002 0.627 0.334 Edotia triloba 0.006 0.009 0.004 0.635 0.250 Pinnixa cylindrica 0.006 0.010 0.005 0.643 0.170 Crepidula spp. 0.006 0.011 0.008 0.651 0.023 * Nassarius albus concensus 0.006 0.011 0.002 0.659 0.017 * Ophioderma brevispina 0.006 0.004 0.010 0.667 0.004 ** Codakia orbicularis 0.006 0.011 0.006 0.675 0.357 Vitrinella floridana 0.006 0.007 0.006 0.682 0.820 Grandidierella bonnieroides 0.005 0.011 0.000 0.690 0.001 *** Bittiolum varium 0.005 0.005 0.009 0.697 0.209 Sayella laevigata 0.005 0.004 0.009 0.705 0.024 * Ericthonius punctatus 0.005 0.003 0.008 0.712 0.141 Chione elevata 0.005 0.009 0.008 0.719 0.542 Conasprella spp. 0.005 0.005 0.006 0.726 0.268 Diopatra sp. 0.005 0.005 0.006 0.732 0.310 Turbonilla sp. 0.005 0.004 0.007 0.739 0.091 . Cinctura hunteria 0.005 0.004 0.007 0.745 0.373 Prunum succineum 0.005 0.003 0.007 0.752 0.100 . Pyrgocythara plicosa 0.005 0.000 0.009 0.758 0.004 ** Monocorophium acherusicum 0.004 0.009 0.000 0.764 0.003 **

64 Supplementary Materials

Amphinomidae sp. 0.004 0.006 0.004 0.770 0.866 Nudibranchia sp. A 0.004 0.007 0.003 0.777 0.474 Erichsonella filiformis floridana 0.004 0.008 0.001 0.783 0.042 * Tellina texana 0.004 0.002 0.008 0.789 0.008 ** Granulina hadria 0.004 0.006 0.004 0.794 0.650 Melanella sp. 0.004 0.000 0.008 0.800 0.053 . Crepidula plana complex 0.004 0.008 0.000 0.806 0.107 Phascolion sp. 0.004 0.006 0.007 0.812 0.527 Gammarus mucronatus 0.004 0.003 0.006 0.818 0.051 . Harrieta faxoni 0.004 0.008 0.001 0.823 0.001 *** Longchaeus suturalis 0.004 0.004 0.006 0.829 0.461 Urosalpinx perrugata 0.004 0.001 0.007 0.834 0.123 Alpheus sp. 0.004 0.005 0.003 0.840 0.970 Brachidontes exustus 0.004 0.008 0.001 0.845 0.001 *** Ameritella versicolor 0.004 0.006 0.002 0.850 0.618 Haminoea elegans 0.004 0.006 0.001 0.856 0.229 Cerithiopsis sp. 0.004 0.004 0.004 0.861 0.792 Globosolembos smithi 0.004 0.003 0.005 0.866 0.295 Transennella conradina 0.003 0.007 0.002 0.871 0.008 ** Cyclostremiscus suppressus 0.003 0.001 0.006 0.876 0.008 ** Leptochelia forresti 0.003 0.004 0.004 0.880 0.877 Vitreolina conica 0.003 0.002 0.006 0.885 0.097 . Dulichiella appendiculata 0.003 0.001 0.006 0.890 0.002 ** Palaemonetes pugio 0.003 0.003 0.004 0.894 0.862 Eulithidium thalassicola 0.003 0.000 0.006 0.899 0.002 ** Bemlos spinicarpus 0.003 0.002 0.005 0.903 0.171 Rubellatoma diomedea 0.003 0.004 0.002 0.907 0.978 Apocorophium simile 0.003 0.004 0.002 0.911 0.974 Cerodrillia spp. 0.003 0.005 0.001 0.915 0.328 Mesokalliapseudes macsweenyi 0.003 0.006 0.000 0.919 0.168 Pitho spp. 0.003 0.005 0.000 0.923 0.004 ** Holothuriidae sp. 0.003 0.001 0.005 0.927 0.021 * Eobrolgus spinosus 0.003 0.003 0.003 0.930 0.364 Idotea sp. A 0.003 0.005 0.001 0.934 0.176 Lyonsia floridana 0.003 0.005 0.000 0.938 0.260 Urosalpinx tampaensis 0.003 0.004 0.001 0.941 0.384 Transennella stimpsoni 0.003 0.005 0.001 0.945 0.349 Vermicularia knorrii 0.002 0.000 0.005 0.948 0.063 . Pyrgospira tampaensis 0.002 0.000 0.005 0.952 0.017 * Hoplomachus propinquus 0.002 0.001 0.004 0.955 0.032 * Phoxocephalidae sp. 0.002 0.004 0.001 0.958 0.723 Pelia mutica 0.002 0.001 0.004 0.961 0.089 . Pteria colymbus 0.002 0.000 0.004 0.964 0.030 * Paracaprella tenuis 0.002 0.001 0.003 0.967 0.106 Meioceras nitidum 0.002 0.000 0.004 0.970 0.001 ***

65 Supplementary Materials

Vexillum exiguum 0.002 0.000 0.004 0.973 0.011 * Boonea impressa 0.002 0.002 0.003 0.976 0.420 Mitromica foveata 0.002 0.000 0.004 0.979 0.032 * Musculus lateralis 0.002 0.000 0.004 0.982 0.070 . Urosalpinx cinerea 0.002 0.001 0.003 0.985 0.173 Nucula proxima 0.002 0.000 0.004 0.987 0.025 * Caecum imbricatum 0.002 0.004 0.000 0.990 0.001 ***

66 Supplementary Materials

5 Table S3.2 Tropic role SIMPER results. Comparisons were made across adjacent estuarine systems, distal systems, and between each distal system and the central system. Abundances are double-Wisconsin-standardized values. Asterisks indicate significance level: <0.001 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1.

Average Average Contribution Abundance Abundance Cumulative WEE vs. CHA % WEE CHA % p 0.102 0.312 0.116 0.346 0.001 *** Epiphyte/Algal Grazer 0.059 0.255 0.278 0.546 0.730 0.054 0.233 0.319 0.730 0.157 Detritivore 0.029 0.105 0.125 0.829 0.938 Parasite 0.028 0.026 0.058 0.924 1.000 Predator 0.022 0.070 0.103 1.000 1.000

Average Average Contribution Abundance Abundance Cumulative CHA vs. HOM % CHA HOM % p Filter Feeder 0.059 0.319 0.227 0.229 0.024 * Parasite 0.059 0.058 0.125 0.457 0.169 Epiphyte/Algal Grazer 0.045 0.278 0.280 0.631 1.000 Detritivore 0.037 0.125 0.145 0.773 0.656 Omnivore 0.037 0.116 0.120 0.915 1.000 Predator 0.022 0.103 0.101 1.000 1.000

Average Average Contribution Abundance Abundance Cumulative HOM vs. CRY % HOM CRY % p Predator 0.062 0.101 0.210 0.208 0.106 Parasite 0.061 0.125 0.119 0.414 0.093 . Epiphyte/Algal Grazer 0.050 0.280 0.247 0.581 0.994 Detritivore 0.047 0.145 0.142 0.739 0.099 . Filter Feeder 0.045 0.227 0.156 0.889 0.694 Omnivore 0.033 0.120 0.126 1.000 1.000

Average Average Contribution Abundance Abundance Cumulative CRY vs. WAC % CRY WAC % p Epiphyte/Algal Grazer 0.079 0.247 0.102 0.236 0.004 ** Omnivore 0.062 0.126 0.240 0.420 0.509 Predator 0.058 0.210 0.281 0.592 0.323 Parasite 0.054 0.119 0.149 0.753 0.507 Detritivore 0.051 0.142 0.040 0.906 0.028 *

67 Supplementary Materials

Filter Feeder 0.032 0.156 0.188 1.000 1.000

Average Average Contribution Abundance Abundance Cumulative WEE vs. HOM % WEE HOM % p Omnivore 0.099 0.312 0.120 0.341 0.001 *** Parasite 0.057 0.026 0.125 0.538 0.281 Epiphyte/Algal Grazer 0.052 0.255 0.280 0.715 0.980 Filter Feeder 0.035 0.233 0.227 0.835 0.995 Detritivore 0.030 0.105 0.145 0.938 0.929 Predator 0.018 0.070 0.101 1.000 1.000

Average Average Contribution Abundance Abundance Cumulative HOM vs. WAC % HOM WAC % p Predator 0.090 0.101 0.281 0.227 0.001 *** Epiphyte/Algal Grazer 0.089 0.280 0.102 0.452 0.001 *** Omnivore 0.063 0.120 0.240 0.610 0.438 Parasite 0.059 0.125 0.149 0.760 0.165 Detritivore 0.053 0.145 0.040 0.893 0.009 ** Filter Feeder 0.042 0.227 0.188 1.000 0.838

Average Average Contribution Abundance Abundance Cumulative WEE vs. WAC % WEE WAC % p Predator 0.105 0.070 0.281 0.278 0.001 *** Epiphyte/Algal Grazer 0.077 0.255 0.102 0.481 0.003 ** Parasite 0.063 0.026 0.149 0.647 0.069 . Omnivore 0.062 0.312 0.240 0.811 0.454 Filter Feeder 0.039 0.233 0.188 0.914 0.954 Detritivore 0.032 0.105 0.040 1.000 0.855

68 Supplementary Materials

6 Supplementary Figures

Supplementary Figure 1. Concentrations of a. chlorophyll-a, b. total phosphorus, and c. total nitrogen in the water column of sites across the latitudinal gradient sampled. Points represent means of 24 months of monthly point samples taken during a concurrent seagrass and water quality monitoring effort that spanned four years (2010-2014) and overlapped temporally with the present effort (2014). Dashed lines represent the moving average concentration across latitude. Note the different y-axis scales.

Supplementary Figure 2. Selected metrics of vegetation complexity collected from sites across the latitudinal gradient sampled. Points represent means of sub-samples collected during the present effort (2014) and during previous monitoring efforts at the same sites (2010-2013). Biomass values are total amounts of vegetative material collected per sample (0.29 m2 sampling area). Dashed lines represent the moving average across latitude. Shoot heights, leaf widths, and shoot density are for Thalassia testudinum. Note the different y-axis scales.

Supplementary Figure 3. Plot of two-dimensional non-metric multidimensional scaling based on taxonomic data summarized at the species level. Colors of points represent the latitude of the sampling location, with darker colors representing higher latitude.

Supplementary Figure 4. Plots of two-dimensional non-metric multidimensional scaling based on taxonomic data summarized at the family level. WEE = Weeki Wachee, CHA = Chassahowitzka, HOM = Homosassa, CRY = Crystal River, WAC = Waccasassa. Colors in indicate relative levels of long-term total phosphorus (TP) concentrations in the water column, with lighter colors indicating lower TP. Sizes of points represent the Shannon-Weaver diversity of samples, with larger sizes indicating a higher diversity value.

Supplementary Figure 5. Plots of two-dimensional non-metric multidimensional scaling based on taxonomic data summarized at the family level. WEE = Weeki Wachee, CHA = Chassahowitzka, HOM = Homosassa, CRY = Crystal River, WAC = Waccasassa. Colors of points in represent the latitude of the sampling location, with darker colors representing higher latitude. Sizes of points represent the Shannon-Weaver diversity of samples, with larger sizes indicating a higher diversity value.

69 ) 1 − 7 a.

6

5

4

3

2

1 Chlorophyll−a Concentration (µg L Concentration Chlorophyll−a ) 1 − 30 b.

25

20

15

10

5 Total Phosphorus (µg L Concentration Total ) 1 − 550 c.

500

450

400

350 Total Nitrogen Concentration (µg L Nitrogen Concentration Total

28.5 28.6 28.7 28.8 28.9 29.0 29.1 Latitude (Degrees) Aboveground Biomass (g DW) Belowground Biomass (g DW) T. testudinum Biomass (g DW) S. filiforme Biomass (g DW) Macroalgal Biomass (g DW)

80 25

40 80 80 20 60 30 60 60 15

40 20

40 40 10

10 20 20 5 20

0 0 0

2 T. testudinum % Cover Vegetative Diversity (H') Shoot Height (mm) Leaf Width (mm) Shoot Density (shoots/m ) 700 1000 100

1.0 6

600 800 0.8 80 500 5

600 0.6 400 60

4 0.4 400 300

40 0.2 200 200 3

0.0 100 0

28.5 28.6 28.7 28.8 28.9 29.0 29.1 28.5 28.6 28.7 28.8 28.9 29.0 29.1 28.5 28.6 28.7 28.8 28.9 29.0 29.1 28.5 28.6 28.7 28.8 28.9 29.0 28.5 28.6 28.7 28.8 28.9 29.0 29.1

Latitude (Degrees) 1.0 Latitude k = 2 stress = 0.21 distance = bray 28.5 28.6 28.7 28.8 28.9 0.5 29.1

0.0 NMDS2

−0.5

−1.0

−1.0 −0.5 0.0 0.5 1.0

NMDS1 1.0 Estuary k = 2 stress = 0.235 distance = bray WEE CHA HOM CRY WAC 0.5

0.0 NMDS2

−0.5

Shannon 3.1 −1.0 2

−1.0 −0.5 0.0 0.5 1.0

NMDS1 1.0 Latitude k = 2 stress = 0.235 distance = bray 28.5 28.6 28.7 28.8 28.9 0.5 29.1

0.0 NMDS2

−0.5

−1.0

−1.0 −0.5 0.0 0.5 1.0

NMDS1

Top View