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THURSDAY , MAY 3 1, 2 0 18 C-07 Thematic Poster S206 Vol. 49 No. 5 Supplement MEDICINE & SCIENCE IN SPORTS & EXERCISE® C-07 Thematic Poster - Carbohydrate and Fat similar between men and women. Perception of effort and heart rate increased over time, but did not differ between men and women [time x sex p≥0.26, effect of sex, Metabolism p≥0.62]. Thursday, May 31, 2018, 8:00 AM - 10:00 AM CONCLUSIONS: The notion that women rely more upon lipids during exercise was Room: CC-Lower level L100C not supported by our data in fed subjects. Men and women who perform prolonged exercise after a light meal, as is typical, are likely to use similar amounts of fats 1068 Chair: Sara Campbell, FACSM. Rutgers University, New and carbohydrates. This underscores the importance of nutritional status in studies comparing men and women. Brunswick, NJ. (No relevant relationships reported) 1069 Board #1 May 31 8:00 AM - 10:00 AM Postprandial Lipemic Responses After A High-fat Meal And Low- Or High-intensity Interval Exercise Racine R. Emmons, Michael A. Figueroa, Toni T. LaSala. William Paterson University, Wayne, NJ. (Sponsor: Gordon Schmidt, FACSM) (No relevant relationships reported) Evidence suggests that high-intensity interval training can be as beneficial, or superior to traditional low to moderate intensity, long duration exercise in clearing postprandial triglycerides (TG).PURPOSE: To compare the effect of exercise intensity on postprandial lipemic responses after a high fat meal. METHODS: Maximal oxygen consumption (VO2) was measured in 13 participants (9 male, 4 female). There were no differences between males and females among age (males: 24±2 years; females 23±2 years), height (1.74 ± 0.04 meters vs. 1.69 ± 0.09 meters, respectively), or VO2max 1071 Board #3 May 31 8:00 AM - 10:00 AM (41.42 ± 3.87 mL/kg/min vs. 36.97 ± 1.39 mL/kg/min, respectively.) Males weighed No Lipolytic Suppression With Pre Exercise heavier than females (78.9 ± 11.2 kg vs. 65.3 ±2.1 kg, p = 0.04). In a randomized crossover design, participants returned on two separate occasions where they arrived Carbohydrate Regardless of its Glycemic Index fasting and consumed a high-fat milkshake made from premium chocolate ice cream Daniel A. Baur1, Brandon D. Willingham2, Smith M. Kyle2, and heavy whipping cream, delivering 1.3 g/kg bodyweight of fat. Participants Kisiolek N. Jacob2, Morrissey C. Margaret2, Ragland J. Tristan2, performed either low intensity exercise (LE) on a treadmill at a self-selected pace Saracino Patrick2, Ormsbee J. Michael, FACSM2. 1Elon to elicit a heart rate (HR) of 40-60% of heart rate reserve (HRR) for 30 minutes or University, Elon, NC. 2Florida State University, Tallahassee, FL. perform high-intensity interval exercise (HE) of 8 repetitions of 30 second sprints (Sponsor: Michael Ormsbee, FACSM) on a treadmill with 90 second active rest. Participants returned on a non-consecutive (No relevant relationships reported) test day to perform the opposite test. Blood was sampled via fingerstick forTG at baseline (T0), 1 hour (1H), 3 hours (3H), and 5 hours (5H) postprandially. Area under the curve (AUC) determined the postprandial TG response via the trapezoid INTRODUCTION: It is well-documented that ingesting carbohydrate prior to exercise method. Comparisons among TG at each time point and AUC were determined via reduces fat oxidation, and that this effect is attenuated with low glycemic index paired samples t-tests. Significance was set atp <0.05. RESULTS: No differences carbohydrates. However, it is yet to be established whether these metabolic effects are were found among baseline, 1H, or 3H TG values between the exercise conditions primarily the result of alterations in the mobilization of free fatty acids (FFA) from (p >0.05). 5H TG and AUC were lower in LE compared to HE (5H: 149.54 ± 113.18 adipose tissue (i.e. lipolysis) or whether these effects impact exercise performance. mg/dL vs. 195.85 ± 117.14 mg/dL, respectively, t(13)= -3.384, p=0.005, d=0.402; PURPOSE: To determine the impact of pre-exercise carbohydrate of different glycemic AUC: 430.39 ± 269.64 mg/dL vs. 508.12 ± 256.97 mg/dL, respectively, t(13)=-2.212, indices on subcutaneous abdominal adipose tissue (SCAAT) metabolism and running p=0.04,d=0.295). Normalized peak TG values were not significantly different across performance. METHODS: Ten trained male runners completed three experimental trials consisting of 30 min at 60% VO , 30 min at 75% VO , and a 5-km time trial groups. CONCLUSIONS: LE resulted in lower postprandial lipemic responses and 2max 2max lower peak compared to HE. The relatively short HE session may have not been (TT). Thirty min prior to exercise, participants consumed one of three beverages: 1) sufficient in duration to clear postprandial TG. 75 g low glycemic index modified starch supplement (UCAN), 2) 75 g high glycemic index glucose-based supplement (G), or 3) a non-caloric placebo (PL). SCAAT lipolysis was assessed via microdialysis. RESULTS: Prior to exercise, blood glucose 1070 Board #2 May 31 8:00 AM - 10:00 AM and insulin were elevated with G vs. PL (+53.0 ± 21.3 mg∙dL-1 [SD]; p = 0.000; +33.9 Similar Substrate Use During Prolonged Cycling in ± 11.0 µU∙mL-1; p = 0.000) and G vs. UCAN (+36.6 ± 24.9 mg∙dL-1; p = 0.00007; Men and Women +25.2 ± 11.0 µU∙mL-1; p = 0.000), respectively. Fat oxidation was attenuated, and carbohydrate oxidation increased prior to exercise with G vs. PL (-0.06 ± 0.06 g∙min-1; Beth W. Glace, Ian J. Kremenic, Malachy P. McHugh, FACSM. p = 0.005; +0.18 ± 0.07 g∙min-1; p < 0.0001) and G vs. UCAN (-0.06 ± 0.05 g∙min-1; THURSDAY, MAY 31, 2018 31, MAY THURSDAY, Lenox Hill Hospital, New York, NY. (Sponsor: Malachy McHugh, p = 0.004; +0.18 ± 0.14 g∙min-1; p < 0.0001). There were no differences in SCAAT FACSM) lipolysis or running performance. CONCLUSIONS: Pre-exercise carbohydrate results (No relevant relationships reported) in metabolic effects favoring carbohydrate utilization, and these effects are attenuated with low glycemic index carbohydrate. However, these effects are not the result of PURPOSE: It is generally accepted that, compared to men, women rely more upon alterations in SCAAT lipolysis, nor do they affect exercise performance. lipids as a substrate during moderate intensity exercise. However, most studies were conducted in a fasting condition, which is not how prolonged exercise is typically 1072 Board #4 May 31 8:00 AM - 10:00 AM performed. The purpose of this study was to compare substrate use between sexes in well-trained cyclists during prolonged exercise after a standardized breakfast. Overload Alters Skeletal Muscle Glucose Utilization METHODS: Well-trained cyclists [18 women, 16 men] were recruited. Subjects but not Glucose Uptake or Hypertrophy in Insulin- reported to our lab twice. On the first day they performed a VO2max test. At 7:30 a.m. Resistant Mice on a subsequent day, they drank a standardized breakfast of a liquid meal replacement Luke Weyrauch1, Shawna McMillin1, Kristen Turner2, Carol which provided 6 kcals/kg. At 9 a.m. they pedaled on their own bikes for 2 hours at Witczak1. 1East Carolina University, Greenville, NC. 2University their ventilatory threshold [~65% VO ] with 5,1-min sprints interspersed throughout. 2max of Iowa, Iowa City, IA. Water was provided at a rate of 1% body mass/h. Ventilatory gas exchange was measured every 20 min to calculate RER. Descriptive data were compared using (No relevant relationships reported) independent t-tests, ventilatory data were compared using repeated measures ANOVA. Resistance exercise training/chronic muscle loading induces skeletal muscle RESULTS: Men and women were of similar age [38 yrs, p=0.92] and VO2max was typical of well-trained, recreational cyclists: 56 ml/kg/min for men, and 47 ml/kg/min hypertrophy and improves systemic glucose homeostasis in individuals with type 2 for women. Body mass declined by 1% and did not differ between sexes [p=0.98]. diabetes. Surprisingly, it is presently unknown if the improvement in glycemic control RER declined during exercise [p<0.001] but there was no difference between sexes is due to an increase in muscle glucose uptake. PURPOSE: To determine if chronic [p=0.38] and no time x sex interaction [p=0.78], indicating that substrate usage was muscle loading (overload)-induced muscle glucose uptake is impaired by insulin resistance. METHODS & RESULTS: Male C57BL/6J mice (6 wks old) were fed ACSM May 29 – June 2, 2018 Minneapolis, Minnesota Official Journal of the American College of Sports Medicine Vol. 49 No. 5 Supplement S207 a low fat diet (LFD) or a 60%kcal high fat diet (HFD) for 12 wks to induce insulin accelerated cellular ageing. Long-term physical activity promotes healthy metabolic resistance. Plantaris muscle overload was elicited by unilateral ablation of the distal and oxidative profiles. The effects of prolonged physical activity however, are 2/3 of the gastrocnemius and soleus. The contralateral leg was sham-operated. Muscles unknown. were weighed 5 days later. Overload increased muscle mass ~40% in both LFD and Purpose HFD mice. To assess glucose uptake, muscles were incubated in [3H]-2-deoxyglucose. This study investigated the effects of prolonged physical activity on oxidative and Overload increased muscle glucose uptake ~80% in both LFD [Sham: 0.52±0.02; metabolic stress in healthy adults participating in a 260-km wilderness canoeing Overload: 0.91±0.04 (µmol/g muscle/h)] and HFD mice [Sham: 0.49±0.04; Overload: expedition. 0.86±0.09 (µmol/g muscle/h)], showing that overload stimulates glucose uptake Methods independent of insulin resistance.
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