Polish Journal of Natural Sciences
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
First Records of a Powdery Mildew Fungus Sawadaea Bicornis (Wallr.)
УДК 582.282.112(477) В.П. ГЕЛЮТА 1, В.В. ДЖАГАН 2, О.О. СЕНЧИЛО 2 1 Інститут ботаніки імені М.Г. Холодного НАН України Україна, 01601 м. Київ, вул. Терещенківська, 2 2 Навчально-науковий центр «Інститут біології», Київський національний університет імені Тараса Шевченка Україна, 01601 м. Київ, вул. Володимирська, 64 ПЕРШІ ЗНАХІДКИ БОРОШНИСТОРОСЯНОГО ГРИБА SAWADAEA BICORNIS (WALLR.) HOMMA НА ACER VELUTINUM BOISS. В УКРАЇНІ Наведено інформацію про перші знахідки в Україні борошнисторосяного гриба Sawadaea bicornis (Wallr.) Homma на інтродукованому декоративному клені (Acer velutinum Boiss.). Уперше гриб виявлено у 2014 р. на території Ботаніч- ного саду імені акад. О.В. Фоміна (Київ). Його розвиток спостерігали тут і наступного року. Ураження A. velutinum борошнистою росою не було значним, міцелій гриба у вигляді дифузних сіруватих плям був добре помітний з верхнього боку листків. Відзначено утворення плодових тіл. Знахідка S. bicornis на A. velutinum є новою не лише для території України, а й для Європи. Очевидно, ця знахідка є свідченням того, що інтродуковані рослини можуть уражатися місцевими расами борошнисторосяних грибів, які розвиваються на споріднених аборигенних видах рослин-жи ви те лів. Ключові слова: Ascomycota, Erysiphales, Sawadaea tulasnei, декоративна рослина, Sapindaceae, Ботанічний сад імені акад. О.В. Фоміна, Крим, Нікітський ботанічний сад. Борошнисторосяні гриби (Erysiphales, Ascomy- на кінському каштані та ще п’яти видах роду cota) є облігатними паразитами судинних рос- Aesculus L. [3], E. elevata (Burrill) U. Braun et лин, переважно дводольних. За останніми да- S. Takam. на катальпі [13], E. magnifica (U. Braun) ними [11], вони уражують понад 10 тис. видів U. Braun et S. Takam. на 11 видах магнолій [9], рослин, однак кожен рік у світі реєструють E. -
PCR Detection of Pseudoperonospora Humuli and Podosphaera Macularis in Humulus Lupulus
Plant Protect. Sci. Vol. 41, No. 4: 141–149 PCR Detection of Pseudoperonospora humuli and Podosphaera macularis in Humulus lupulus JOSEF PATZAK Hop Research Institute Co., Ltd., Žatec, Czech Republic Abstract PATZAK J. (2005): PCR detection of Pseudoperonospora humuli and Podosphaera macularis in Humulus lupulus. Plant Protect. Sci., 41: 141–149. Hop downy mildew (Pseudoperonospora humuli) and hop powdery mildew (Podosphaera macularis) are the most important pathogens of hop (Humulus lupulus). The early detection and identification of these pathogens are often made difficult by symptomless or combined infection with another pathogens. Molecular analysis of internal transcribed spacer (ITS) regions of rDNA is a novel and very effective method of species determina- tion. Therefore, specific PCR assays were developed to detect the pathogens Pseudoperonospora humuli and Podosphaera macularis in naturally infected hop plants. The specific PCR primer combinations P1 + P2 and S1 + S2 amplified specific fragments from Pseudoperonospora humuli and Podosphaera macularis, respectively, and did not cross-react with hop DNA nor with DNA from other fungi. PCR primer combinations R1 + R2 and R3 + R4 could be used in multiplex PCR detection of Pseudoperonospora humuli, Podosphaera macularis, Verticillium albo-atrum and Fusarium sambucinum. Phylogenetic relationships were inferred for 42 species of the Erysiphales from nuclear rDNA (ITS1, 5.8S, ITS2). The molecular characterisation and phylogenetic analy- ses confirmed the species identification of hop powdery mildew. The PCR assays used in this study proved to be accurate and sensitive for detection, identification, classification and disease-monitoring of the major hop pathogens. Keywords: hop powdery mildew; hop downy mildew; internal transcribed spacers (ITS); PCR detection; phylo- genetic analysis Hop (Humulus lupulus L.) is a dioecious, peren- disease occurring worldwide. -
Preliminary Classification of Leotiomycetes
Mycosphere 10(1): 310–489 (2019) www.mycosphere.org ISSN 2077 7019 Article Doi 10.5943/mycosphere/10/1/7 Preliminary classification of Leotiomycetes Ekanayaka AH1,2, Hyde KD1,2, Gentekaki E2,3, McKenzie EHC4, Zhao Q1,*, Bulgakov TS5, Camporesi E6,7 1Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China 2Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand 3School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand 4Landcare Research Manaaki Whenua, Private Bag 92170, Auckland, New Zealand 5Russian Research Institute of Floriculture and Subtropical Crops, 2/28 Yana Fabritsiusa Street, Sochi 354002, Krasnodar region, Russia 6A.M.B. Gruppo Micologico Forlivese “Antonio Cicognani”, Via Roma 18, Forlì, Italy. 7A.M.B. Circolo Micologico “Giovanni Carini”, C.P. 314 Brescia, Italy. Ekanayaka AH, Hyde KD, Gentekaki E, McKenzie EHC, Zhao Q, Bulgakov TS, Camporesi E 2019 – Preliminary classification of Leotiomycetes. Mycosphere 10(1), 310–489, Doi 10.5943/mycosphere/10/1/7 Abstract Leotiomycetes is regarded as the inoperculate class of discomycetes within the phylum Ascomycota. Taxa are mainly characterized by asci with a simple pore blueing in Melzer’s reagent, although some taxa have lost this character. The monophyly of this class has been verified in several recent molecular studies. However, circumscription of the orders, families and generic level delimitation are still unsettled. This paper provides a modified backbone tree for the class Leotiomycetes based on phylogenetic analysis of combined ITS, LSU, SSU, TEF, and RPB2 loci. In the phylogenetic analysis, Leotiomycetes separates into 19 clades, which can be recognized as orders and order-level clades. -
Hyperparasites of Erysiphales Fungi in the Urban Environment
POLISH JOURNAL OF NATURAL SCIENCES Abbrev.: Pol. J. Natur. Sc., Vol 27(3): 289–299, Y. 2012 HYPERPARASITES OF ERYSIPHALES FUNGI IN THE URBAN ENVIRONMENT Ewa Sucharzewska, Maria Dynowska, Elżbieta Ejdys, Anna Biedunkiewicz, Dariusz Kubiak Department of Mycology University of Warmia and Mazury in Olsztyn Key words: Ampelomyces, hyperparasites, fungicolous fungi, powdery mildew, transport pollu- tion effects, anthropopressure. Abstract This manuscript presents data on the occurrence of hyperparasitic fungi colonizing the mycelium of selected species of Erysiphales: Erysiphe alphitoides, E. hypophylla, E. palczewskii, Golovinomyces sordidus, Podosphaera fusca and Sawadaea tulasnei in the urban environment. In the paper the effect of hyperparasites on the development of fungal hosts at diversified level of transport pollution is emphasized. Over a three-years experiment, the presence of hyperparasites was confirmed on all analyzed Erysiphales species, with prevailing species from the genus Ampelomyces. The representa- tives of other genera: Alternaria, Aureobasidium, Cladosporium, Stemphylium and Tripospermum were also observed on mycelium of E. alphitoides and E. palczewskii. The hyperparasites occurred only on stations situated at the main roads were found not to affect the extent of plant infection by fungi of the order Erysiphales, but reduced the number of chasmothecia. NADPASOŻYTY GRZYBÓW Z RZĘDU ERYSIPHALES W ŚRODOWISKU MIEJSKIM Ewa Sucharzewska, Maria Dynowska, Elżbieta Ejdys, Anna Biedunkiewicz, Dariusz Kubiak Katedra Mykologii Uniwersytet Warmińsko-Mazurski w Olsztynie Słowa kluczowe: Ampelomyces, nadpasożyty, mączniaki prawdziwe, zanieczyszczenia komunikacyjne, antropopresja. Address: Ewa Sucharzewska, University of Warmia and Mazury, ul. Michała Oczapowskiego 1A, 10-719 Olsztyn, Poland, phone: +48 (89) 523 42 98, e-mail: [email protected] 290 Ewa Sucharzewska et al. -
Shropshire Fungus Checklist 2010
THE CHECKLIST OF SHROPSHIRE FUNGI 2011 Contents Page Introduction 2 Name changes 3 Taxonomic Arrangement (with page numbers) 19 Checklist 25 Indicator species 229 Rare and endangered fungi in /Shropshire (Excluding BAP species) 230 Important sites for fungi in Shropshire 232 A List of BAP species and their status in Shropshire 233 Acknowledgements and References 234 1 CHECKLIST OF SHROPSHIRE FUNGI Introduction The county of Shropshire (VC40) is large and landlocked and contains all major habitats, apart from coast and dune. These include the uplands of the Clees, Stiperstones and Long Mynd with their associated heath land, forested land such as the Forest of Wyre and the Mortimer Forest, the lowland bogs and meres in the north of the county, and agricultural land scattered with small woodlands and copses. This diversity makes Shropshire unique. The Shropshire Fungus Group has been in existence for 18 years. (Inaugural meeting 6th December 1992. The aim was to produce a fungus flora for the county. This aim has not yet been realised for a number of reasons, chief amongst these are manpower and cost. The group has however collected many records by trawling the archives, contributions from interested individuals/groups, and by field meetings. It is these records that are published here. The first Shropshire checklist was published in 1997. Many more records have now been added and nearly 40,000 of these have now been added to the national British Mycological Society’s database, the Fungus Record Database for Britain and Ireland (FRDBI). During this ten year period molecular biology, i.e. DNA analysis has been applied to fungal classification. -
Fungi Determined in Ankara University Tandoğan Campus Area (Ankara-Turkey)
http://dergipark.gov.tr/trkjnat Trakya University Journal of Natural Sciences, 20(1): 47-55, 2019 ISSN 2147-0294, e-ISSN 2528-9691 Research Article DOI: 10.23902/trkjnat.521256 FUNGI DETERMINED IN ANKARA UNIVERSITY TANDOĞAN CAMPUS AREA (ANKARA-TURKEY) Ilgaz AKATA1*, Deniz ALTUNTAŞ1, Şanlı KABAKTEPE2 1Ankara University, Faculty of Science, Department of Biology, Ankara, TURKEY 2Turgut Ozal University, Battalgazi Vocational School, Battalgazi, Malatya, TURKEY *Corresponding author: ORCID ID: orcid.org/0000-0002-1731-1302, e-mail: [email protected] Cite this article as: Akata I., Altuntaş D., Kabaktepe Ş. 2019. Fungi Determined in Ankara University Tandoğan Campus Area (Ankara-Turkey). Trakya Univ J Nat Sci, 20(1): 47-55, DOI: 10.23902/trkjnat.521256 Received: 02 February 2019, Accepted: 14 March 2019, Online First: 15 March 2019, Published: 15 April 2019 Abstract: The current study is based on fungi and infected host plant samples collected from Ankara University Tandoğan Campus (Ankara) between 2017 and 2019. As a result of the field and laboratory studies, 148 fungal species were identified. With the addition of formerly recorded 14 species in the study area, a total of 162 species belonging to 87 genera, 49 families, and 17 orders were listed. Key words: Ascomycota, Basidiomycota, Ankara, Turkey. Özet: Bu çalışma, Ankara Üniversitesi Tandoğan Kampüsü'nden (Ankara) 2017 ve 2019 yılları arasında toplanan mantar ve enfekte olmuş konukçu bitki örneklerine dayanmaktadır. Arazi ve laboratuvar çalışmaları sonucunda 148 mantar türü tespit edilmiştir. Daha önce bildirilen 14 tür dahil olmak üzere 17 ordo, 49 familya, 87 cinse mensup 162 tür listelenmiştir. Introduction Ankara, the capital city of Turkey, is situated in the compiled literature data were published as checklists in center of Anatolia, surrounded by Çankırı in the north, different times (Bahçecioğlu & Kabaktepe 2012, Doğan Bolu in the northwest, Kırşehir, and Kırıkkale in the east, et al. -
New Records and New Host Plants of Powdery Mildews (Erysiphales) from Idaho and Oregon (USA)
Schlechtendalia 27 (2013) New records and new host plants of powdery mildews (Erysiphales) from Idaho and Oregon (USA) Uwe BRAUN & S. Krishna MOHAN Abstract: Braun, U. & Mohan, S. K. 2013: New records and new host plants of powdery mildews (Erysiphales) from Idaho and Oregon (USA). Schlechtendalia 27: 7–10. In the course of routine examinations of powdery mildews collected in Idaho and Oregon, USA, some of the identified species proved to be new to North America, in some cases on new host plants. Leveillula papilionacearum and L. picridis are first records from the USA. Astragalus filipes, Dalea ornata and D. searlsiae are new hosts for Leveillula papilionacearum. Enceliopsis, Heliomeris and Machaeranthera are new host genera for L. picridis. Additional records refer to powdery mildew species which have been rarely reported from North America or first records on certain hosts. Zusammenfassung: Braun, U. & Mohan, S. K. 2013: Neufunde und neue Wirtsarten von Mehltaupilzen (Erysiphales) aus Idaho und Oregon (USA). Schlechtendalia 27: 7–10. Im Verlauf von Routineuntersuchungen an in Idaho und Oregon, USA, gesammelten Mehltaupilzen erwiesen sich einige der identifizierten Arten als Neunachweise für Nordamerika, in einigen Fällen auf neuen Wirtsarten. Erstnachweise für die USA umfassen Leveillula papilionacearum und L. picridis; Astragalus filipes, Dalea ornata und D. searlsiae sind neue Wirtsarten für Leveillula papilionacearum, und Enceliopsis, Heliomeris und Machaeranthera sind neu nachgewiesene Wirtsgattungen für L. picridis. Weitere Funde beziehen sich auf Arten, die selten aus Nordamerika angegeben worden sind, bzw. auf Neuangaben auf bestimmten Wirten. Key words: Erysiphaceae, North America, novelties, new hosts. Published online 6 Dec. 2013 Powdery mildews (Erysiphales) are an important group of plant pathogenic fungi, worldwide in distribution, and occur on a wide range of hosts including numerous economically important cultivated plants. -
Occurrence of the Fungi from the Genus Ampelomyces – Hyperparasites of Powdery Mildews (Erysiphales) Infesting Trees and Bushes in the Municipal Environment
Vol. 80, No. 2: 169-174 , 2011 ACTA SOCIETATIS BOTANICORUM POLONIAE 169 OCCURRENCE OF THE FUNGI FROM THE GENUS AMPELOMYCES – HYPERPARASITES OF POWDERY MILDEWS (ERYSIPHALES) INFESTING TREES AND BUSHES IN THE MUNICIPAL ENVIRONMENT EWA SUCHARZEWSKA , M ARIA DYNOWSKA , A NETA BOŻENA KEMPA Department of Mycology, University of Warmia and Mazury in Olsztyn Oczapowskiego 1A,10-719 Olsztyn-Kortowo, Poland e-mail: [email protected] (Received: March 23, 2010. Accepted: July 19, 2010) ABSTRACT The studies refer to the phenomenon of hyperparasitism in the municipal environment. The paper presents the occurrence of fungi of the genus Ampelomyces on Erysiphales – important group of phytopathogenic fungi. For the first time in Poland analyzed degree of infestation of Erysiphales mycelium by Ampelomyces and effect of the hyperparsites on the degree of infestation plants by Erysiphales . The high participation of the Ampelomyces was noted in each year of the study. Substantial differences were noted in the occurrence of Ampelomyces depending on the developmental stage of the host fungi and considerable differences in the prevalence of the hyperparasites on particular Erysiphales species. In all cases examined , the mean index of infestation of host plants by Erysiphales was higher than the mean degree of infestation of powdery mildew mycelium by Ampelomyces . The results indicate that under natural conditions they do not play any significant role in the reduction of the degree of infestation of host plants by Erysiphales and do not disturb drastically their life cycle. KEY WORDS: Ampelomyces , Erysiphales, hyperparasites, municipal environment. INTRODUCTION Kiss at al. 2004). In turn , still little attention is paid to the ecology of those parasites , their occurrence and effect on Fungi from the genus Ampelomyces (Ces. -
Erysiphe Azaleae (Erysiphales) – a New Species of Powdery Mildew for Slovakia and Further Records from the Czech Republic
CZECH MYCOL. 58(3–4): 189–199, 2006 Erysiphe azaleae (Erysiphales) – a new species of powdery mildew for Slovakia and further records from the Czech Republic 1 2 KAMILA BACIGÁLOVÁ and JAROSLAVA MARKOVÁ 1Institute of Botany of the Slovak Academy of Sciences, Department of Non-vascular Plants, Dúbravská cesta 14, SK – 845 23 Bratislava, Slovakia [email protected] 2 Department of Botany, Charles University, Benátská 2, CZ –128 01 Praha 2, Czech Republic [email protected] Bacigálová K. and Marková J. (2006): Erysiphe azaleae (Erysiphales) – a new species of powdery mildew for Slovakia and further records from the Czech Re- public. – Czech Mycol. 58(3–4): 189–199. Erysiphe azaleae, a powdery mildew growing on deciduous Rhododendron species is reported from the Slovak and Czech Republics. A detailed description is given and its distribution in Europe is discussed. Key words: Erysiphaceae, powdery mildew, Rhododendron, Azalea, Central Europe Bacigálová K. a Marková J. (2006): Erysiphe azaleae (Erysiphales) – nový druh padlí pro Slovensko a další nálezy v České republice. – Czech Mycol. 58(3–4): 189–199. Erysiphe azaleae, padlí na opadavých druzích rodu Rhododendron, bylo nalezeno na území Slo- venska a České republiky. Je uveden popis druhu a údaje o jeho rozšíření v Evropě. INTRODUCTION Recently an increasing number of reports on the appearance of new powdery mil- dew species (Erysiphales) in various parts of Europe have been published, and the distribution areas have been extended: e. g. Erysiphe vanbruntiana (W.R.Gerard) U. Braun et S. Takam. on Sambucus racemosa and Sambucus nigra, Erysiphe palczewskii (Jacz.) U. Braun et S. -
Full Text [PDF]
® The European Journal of Plant Science and Biotechnology ©2011 Global Science Books Powdery Mildews on Ornamental Trees and Shrubs in Norway Venche Talgø1* • Leif Sundheim1 • Halvor B. Gjærum1 • Maria Luz Herrero1 • Aruppillai Suthaparan2 • Brita Toppe1 • Arne Stensvand1 1 Norwegian Institute for Agricultural and Environmental Research, Plant Health and Plant Protection Division, 1432 Ås, Norway 2 Norwegian University of Life Sciences, Department of Plant and Environmental Sciences, 1432 Ås, Norway Corresponding author : * [email protected] ABSTRACT This paper presents powdery mildew species recorded on woody ornamentals, with special emphasis on the latest arrivals; Erysiphe flexuosa on horse chestnut (Aesculus hippocastanum), Erysiphe syringae-japonicae on lilac (Syringa vulgaris) and Podosphaera spiraeae on white spirea (Spiraea betulifolia). The two former were found in 2006, while the latter was first detected in 2008. Chasmothecia (formerly named cleistothecia) were not found on white spirea until 2010. Several locations seemed to have optimal conditions for development of powdery mildew diseases in 2006. That year the long established Sawadaea bicornis on sycamore maple (Acer pseudo- platanus), was found for the first time on tatarian maple (Acer tataricum ssp. ginnala) and one cultivar from hedge maple (Acer campestre ‘Red Shine’). Also several species and cultivars of Rhododendron had massive attacks of powdery mildew in 2006. In 2010, chasmothecia of E. azaleae were found on severely affected R. ‘Magnifica’ in western -
New Collections of Powdery Mildews (Erysiphales) in Poland
Polish Botanical Journal 47(2): 215–222, 2002 NEW COLLECTIONS OF POWDERY MILDEWS (ERYSIPHALES) IN POLAND AGATA WOŁCZAN´ SKA & WIESŁAW MUŁENKO Abstract: New hosts and records of powdery mildews (Erysiphales) in Poland are discussed. Erysiphe adunca (Wallr.) Fr. was found on a host new for this fungus: Salix repens L. subsp. arenaria (L.) Hiitonen. Two other species – Golovinomyces cichoracea- rum (DC.) V. P. Gelyuta var. cichoracearum (on Chondrilla juncea L.) and Erysiphe syringae Schwein. (on Ligustrum vulgare L.) – were collected on host plants new for Poland. The anamorphic as well as teleomorphic (cleistothecia with ascospores) state of Erysiphe syringae was observed on Ligustrum vulgare and Syringa vulgaris L.; hitherto this fungus has formed mature cleistothecia only in North America. Erysiphe flexuosa (Peck) U. Braun & S. Takamatsu has been introduced from North America to Europe recently. Aesculus pavia L. and A. × carnea Hayne are new hosts for this fungus in Poland. Golovinomyces cichoracearum var. cichoracearum [on Cirsium canum (L.) All.] and Podosphaera spiraeae (Sawada) U. Braun & S. Takamatsu (on Spiraea cf. japonica L.) are recorded on rare host plants. Key words: taxonomy, ecology, parasitic fungi, Ascomycota, Erysiphales, Erysiphe, Golovinomyces, Podosphaera Agata Wołczan´ska & Wiesław Mułenko, Department of General Botany, Maria Curie-Skłodowska University, Akademicka 19, PL-20-033 Lublin, Poland; e-mail: [email protected]; [email protected] INTRODUCTION In recent years an increasing number of reports on observed for two other species: Erysiphe syringae the appearance of new powdery mildew species Schwein. (Braun 1987, 1995) and Erysiphe flexu- (Erysiphales) in Poland and other parts of Europe osa (Peck) U. -
Plant Introduction МІЖНАРОДНИЙ НАУКОВИЙ ЖУРНАЛ • ЗАСНОВАНИЙ У 1999 Р
НАЦІОНАЛЬНА АКАДЕМІЯ НАУК УКРАЇНИ • НАЦІОНАЛЬНИЙ БОТАНІЧНИЙ САД ім. М.М. ГРИШКА 1(69)/2016 Plant introduction МІЖНАРОДНИЙ НАУКОВИЙ ЖУРНАЛ • ЗАСНОВАНИЙ У 1999 р. • ВИХОДИТЬ 4 РАЗИ НА РІК • КИЇВ ЗМІСТ CONTENTS Теорія, методи і практичні аспекти Theory, Methods and Practical Aspects інтродукції рослин of Plant Introduction РАХМЕТОВ Д.Б., ЛЕВЧИК Н.Я., ШПАК Л.М., 3 RAKHMETOV D.B., LEVCHYK N.Ya., SHPAK L.M., ГРАХОВ В.П. , БОЙКО О.М., ЛЮБІНСЬКА А.В., HRAKHOV V.P. , BOIKO O.M. , LYUBINSKA A.V., РАХМЕТОВА С.О., ЗАВГОРОДНІЙ В.М. Інтро- RAKHMETOVA S.O., ZAVHORODNIY V.M. In- дукція рослин Stevia rebaudiana (Bert.) Bertoni в troduction of Stevia rebaudiana (Bert.) Bertoni Національному ботанічному саду ім. М.М. Гриш- plants in M.M. Gryshko National Botanical Garden ка НАН України of the NAS of Ukraine Збереження різноманіття рослин Conservation of Plant Diversity ГОРЄЛОВ О.М., ЧОРНОМАЗ Н.М. Деревна та ча- 18 GORELOV O.M., CHORNOMAZ N.M. Trees and гарникова рослинність наддніпрянських схилів у ме- shrubs vegetation of Dnieper slopes of M.M. Grysh- жах Національного ботанічного саду ім. М.М. Гриш- ko National Botanical Garden of the NAS of Uk- ка НАН України raine Біологічні особливості інтродукованих рослин Biological Peculiarities of Introduced Plants ХАРЧЕНКО І.І. Морфологічні особливості бу- 26 KHARCHENKO I.I. Morphological peculiarities of дови вегетативних органів Camellia oleifera Abel structure of Camellia oleifera Abel vegetative organs ЖИЛА А.І., ТИМЧЕНКО О.Д. Будова коренів 33 ZHILA A.I., TIMCHENKO O.D. The structure та особливості їх росту в Oxalis tetraphylla Cav. and growth characteristics of Oxalis tetraphylla Cav.