Quick viewing(Text Mode)

Study of Some Oxidative Parameters Induced by Drought Stress in Nigella

Study of Some Oxidative Parameters Induced by Drought Stress in Nigella

1392 1 2

3 *2 1 3 2 1 (1392 01/ 28/ : 1391 07/ 10/ : )

: . . . . . . . . Downloaded from jispp.iut.ac.ir at 8:31 IRST on Tuesday September 28th 2021 . .

:

: 250 .( Kocheki and Nasiri Mahalati, 1994 ) .

* [email protected] :

139 2 1 2 12

.( Salem, 2005 ) . .( Jongdee et al ., 2002 ) . . . . .( Fu and Huang, 2001 ) pH ( CAT ) (SOD ) ( APX ) (POX ) ( GR ) SOD .

( H2O2) . . . POX CAT APX : Sundhakcar et al ., 2001 Downloaded from jispp.iut.ac.ir at 8:31 IRST on Tuesday September 28th 2021 ) .( ) . ( sativa . . ( Hoagland and Arnon, 1950 ) Nigella sativa 4 . 2/1 ) . .( 1387 . 13 13 ...

. 4 : . MDA-TBA 600 . MDA . -1 -1 155 mM cm 24 . 24 . . : .

( PEG 6000 ) 6000 5/0 . ( KI ) . 6/0 4/0 2/0 . 1/0 TCA . . 12000 g 15 48 500 500 2 ( pH= )7 100 . 80 1 1 . 1 : . 390 . .( Alexieva et al ., 2001 ) Packer ( MDA ) : : 2/0 . (1969 ) Heath 50 5/0 5 ( ) 1 ( pH= )7 . (1/0 TCA )

Downloaded from jispp.iut.ac.ir at 8:31 IRST on Tuesday September 28th 2021 . EDTA 10000 g 5 20 10000 g . 4 .( .( Gapinska et al ., 2008 ) 5/0 20 ( TCA ) :(EC 1.11.1.6) (CAT ) 30 . ( TBA ) 95 30 . 240 . 10000 g 10 15 (pH= )7 50 . 100 532 ( ) Varian Cary50

139 2 1 2 14

1 1 . 200 30 H2O2 . 30 . 234 40 mM -1 cm -1 -1 -1 M cm .( Velikova et al ., 2000 ) . . 25000 : : (EC1.11.1.7) ( GPX) . (1976 ) Bradford . 1/0 2/77 25 100 ( pH= )7 50 . 100 1 595 . 4 3 470 . : 26 6/ mM -1 cm -1 02/0 . Bates ( 1973 ) .( Plewa et al ., 1991 ) 3 10 ( ) 5 5 :(EC 1.11.1.11) ( APX ) 2 . 10000 g 2 2 . 5/0 (pH= )7 50 100 150 1/0 APX .

Downloaded from jispp.iut.ac.ir at 8:31 IRST on Tuesday September 28th 2021 4 . 1 . . 290 . 20 15 .( Nakano and Asada, 1981 ) 8/2 mM -1 cm -1 520 (EC (LOX ) :1.13.11.12) . . ( 1993 ) Minguez-Mosquera : 25 . 775/2 100 SAS ( pH= 5/5 ) 100 15 15 ...

(B) (A) 1 P≤≤≤ /0 05 .. ( SE ) . LSD . 2/0 4/0 4/0 2/0 D3 D2 D1) . .( 6/0

( B 1 ) % 93 8/ (A 1 ) % 65 8/ . LSD . . Excel : PEG H2O2 . 0 /2 GPX APX . .( A, D 2 ) Downloaded from jispp.iut.ac.ir at 8:31 IRST on Tuesday September 28th 2021 2 GPX CAT . . . GPX CAT APX ) % 91 4/ ( B 2 ) % 81 2/ ( A 2 ) % 24 5/ .( 1 ) MDA . ( D 2 ) % 61 3/ (C 2 6/0 MDA

139 2 1 2 16

( C) (B) (A) 2 < /0 05 . ( SE ) . LSD . ( D) D3 D2 D1) . P .( .( 6/0 4/0 2/0

(2 A ) 6/0 . % 73 8/ Downloaded from jispp.iut.ac.ir at 8:31 IRST on Tuesday September 28th 2021 .( 3 ) Halliwel and ) . .( Gutteridge, 1984 : .( Scandadalius, 1993 ) .( Iturbe-ormaetxe et al ., 1998 ) Ohkawa et al ., 1979; ) .( .( Shulaev and Oliver, 2006 . 17 17 ...

. LSD . 3 . P≤≤≤ /0 05 . ( SE ) .( .( 6/0 4/0 2/0 D3 D2 D1)

.(Ohkawa et al ., 1979; Shulaev and Oliver, 2006 ) Egert and Tevini 2002 Babar Ali et al ., 2005 ) .( .( Tian and Li, 2006 Kubis, 2006 Juan et al ., 2005 1 .( Bandeoglu et al ., 2004 ) MDA . ( )

Downloaded from jispp.iut.ac.ir at 8:31 IRST on Tuesday September 28th 2021 . . . . .( Babar Ali et al ., 2005; Egert and Tevini, 2002 ) MDA . . SOD

H2O2 Phalaenopsis .( Laspina et al., 2005 )

139 2 1 2 18

)

( DNA Sundhacar et al ., ) H2O2 . NADP +/NADPH pH .( 2001 ( )

.( Matysik et al ., 2002; Verbruggen and Hermans, 2008 ) H2O2 .( A 2 B 1 ) .( Chaitanya et al ., 2009 ) .( 3 ) 3 .

H2O2 PEG .( Delauney and Verma, 1993 ) ( Demiral and Turkan, 2005 ) (Abd El Samad and Shaddad, 1997 ) . ( Juan et al ., 2005 ) .

Downloaded from jispp.iut.ac.ir at 8:31 IRST on Tuesday September 28th 2021 ROS . .

tion on growth and stress markers in pea and : wheat. Cell and Environment 24:1337-1344. Babar Ali, M., Hahn, E. G. and Paek, K. Y. (2005) ( 1387 .) . Effects of temperature on oxidative stress defense systems, lipid peroxidation and lipoxygenase ac- . tivity in Phalaenopsis . Plant Physiology and Bio- chemistry 43: 213-223. . Bandeoglu, E., Egidogan, F., Yucel, M. and Avni Abd El Samad, H. M. and Shaddad, M. A. K. (1997) Oktem, H. (2004) Antioxidant responses of shoots tolerance of soybean cultivars. Plant Biology and roots of lentil to NaCl- salinity stress. Plant 39: 263-269. Growth Regulation 42: 69- 77. Alexieva, V., Sergiev, I., Mapelli, S. and Karanov, E. (2001) The effect of drought and ultraviolet radia- 19 19 ...

Kocheki, A. and Nasiri Mahalati, M. (1994) Ecology Bates, L. S. (1973) Rapid determination of free pro- of . 2nd Ed. Jahad Daneshgahi, Mashad. line for water stress studies. Plant Soil 39: 205-207. Kubis, J. (2006) Exogenous spermidine alters in Bradford, M. M. (1976) A rapid and Sensitive Me- different way membrane permeability and lipid thod for Quantitation of Microgram Quantities of peroxidation in water stressed barley . Acta Protein Utilizing the Principle of Protein-dye Physiology Plantarum 28:27- 33. Binding. Analytical Biochemistry 72: 248-254. Laspina, N. V., Groppa, M. D., Tomaro, M. L. and Chaitanya, K. V., Rasineni, G. K. and Reddy, A. R. Benavides, M.P. (2005) Nitric oxide protects sun- (2009) Biochemical responses to drought stress in leaves against Cd-induced oxidative stress. mulberry ( Morus alba L.): evaluation of proline, Plant Science 169:323-330. glycine betaine and abscisic acid accumulation in Matysik, J., Alia, A., Bhalu, B. and Mohanty, P. five cultivars. Acta Physiology Plantarum 31: (2002) Molecular mechanisms of quenching of 437-447. reactive oxygen species by proline under stress in Delauney, A. and Verma, D. (1993). Proline biosyn- plants. Current Science 82:525-532. thesis and degradation in plants. Plant Journal 4: Minguez-Mosquera, M. I., Jaren-Galen, M. and Gar- 215-223. rido-Fernandez, J. (1993) Lipoxygenase activity Demiral, T. and Turkan, I. (2005) Comparative lipid during pepper ripening and processing of . peroxidation, antioxidant defense system and pro- Phytochemistry 32:1103-1108. line content in roots of two rice cultivars differing Nakano, Y. and Asada, K. (1981) Hydrogen peroxide in salt tolerance. Environment and Experimental is scavenged by ascorbate-specific peroxidase in Botany 53: 247-257. spinach choloroplast. Plant and Cell Physiology Egert, M. and Tevini, M. (2002) Influence of drought 22: 867-880. on some physiological parameters symptomatic Ohkawa, H., Ohishi, N. and Yaqi, K. (1979) Assay for oxidative stress in leaves of ( Allium for lipid peroxides in animal tissue by thiobarbi- schoenoprasum ). Environment and Experimental turic acid reaction. Annals of Biochemistry 95: Botany 48: 43-49. 351-358. Fu, J. and Huang, B. (2001) Involvement of Antioxi- Plewa, M. J., Smith, S. R. and Wanger, E. D. (1991) dant and Lipid Peroxidation in the Adaptation of Diethyldithiocarbamate suppresses the plant acti- Two Cool-season Grasses to Localized Drought vation of aromatic amines into mutagens by inhibiting Stress. Environment and Experimental Botany tobacco cell peroxidase. Mutation Research 247:57-64 . 45:105-114. Gapinska, M., Sklodowska, M. and Gabara, B. Salem, M. L. (2005) Immunomodulatory and thera- (2008) Effect of short- and Long-term salinity on peutic properties of the Nigella sativa L. . Inter- the activities of antioxidative enzymes and lipid national Immunopharmacology 5:1749-1770. peroxidation in tomato roots. Acta Physiologu Scandadalius, J. G. (1993) Oxygen stress and supe- Plantarum 30:11-18. roxide dismutase. Plant Physiology 101:7- 12. Halliwel, B. and Gutteridge, J. M. (1984) Oxygen Shulaev, V. and Oliver, D. J. (2006) Metabolic and toxicity, oxygen radicals, transition metal and dis- proteomic markers for oxidative stress, new tools ease. Journal of Biological Chemistry 219: 1-14. for reactive oxygen species research. Plant Physi- Heath, R. L. and Packer, L. (1968) Photoperoxidation

Downloaded from jispp.iut.ac.ir at 8:31 IRST on Tuesday September 28th 2021 ology 141:367-372. in isolated chloroplast, kinetics and stoichiometry Sundhacar, C., Lakshmi, A. and Giridarakumar, S. of fatty acid peroxidation. Archives of Biochemi- (2001) Changes in the Antioxidant Enzyme Effi- stry and Biophysics 125:189-198. cacy in two High Yielding Genotypes of Mulber- Hoagland, D. R. and Arnon, D. J. (1950) The water ry ( Morus alba L.) under NaCl Salinity. Plant culture method for growing plants without soil. Science 161:613-619. 2nd Ed. California Agricultural Experimental Sta- Tian, X. and Li, Y. (2006) Nitric oxide treatment tion, Berkeley. alleviates drought stress in wheat seedlings. Bi- Iturbe-ormaetxe, I., Escuredo, P.R., Arrese-Igor, C. ologia Plantarum 50:775-778. and Becana, M. (1998) Oxidative damage in pea Velikova, V., Yordanov, I. and Edreva, A. (2000) plant exposed to water deficit or paraquat. Plant Oxidative stress and some antioxidant systems in Physiology 116:173-181. acid rain treated bean plants. Protective role of Jongdee B., Fukai, S. and Cooper, M. (2002) exogenous polyamines. Plant Science 151:59-66. water potential and osmotic adjustment as physio- Verbruggen, N. and Hermans, C. (2008) Proline- logical traits to improve drought tolerance in rice. accumulation in plants: a review. Amino acids Field Crops Research 76:153-163. 35:753-759. Juan, M., Rivero, R. M., Romero, L. and Ruiz, J. M. (2005) Evaluation of some nutritional and bio- chemical indicators in selecting salt-resistant to- mato cultivars. Environment and Experimental Botany 54:193-201.