SigmaPlot 14.5 Designed Specifically to Meet the Needs of Scientists, Professional Researchers and Engineers

With an award-winning interface and intuitive wizard technology that guides users step-by-step through the graph creation and data analysis process, SigmaPlot provides the flexibility to create compelling graphs and statistical analysis you simply can’t achieve with basic spreadsheet .

Import Statistical Analysis Macros Help Import multiple sheets Expanded model selection criteria for polynomial Enhancements to polar plots from the Improved search in Help from Excel files. regression, new assumption checking criteria and Polar and Parametric Equations macro. The system. table formatting for correlation tests, numerical Histogram Plus Kernel Density macro added and graph results added to contingency tables to Toolbox

Transforms System Memory Add Value Symbols New functions in the Transform Language for Status bar shows remaining memory Value symbols can now be easily added to symbol matrix computations. Transform Samples file and bar graphs update with new transforms for orthogonal Histograms regression, regression with equality constraints, User Interface Improvements Passing-Bablok regression, and chi-square Scaling options for counts and options for either goodness-of-fit test for category data. left-edge or right-edge binning are available for Plots can be deleted from Graph Properties, the histograms from the Histogram Wizard and from Add Axis default has been changed to the Y Axis, statistical procedures. Align Objects is now available from the right mouse Subsections In Notebooks menu, and mini-toolbars have been improved Notebooks expanded to include one or more Smoothers when drawing arrows subsections under each section to better organize Reports available for 2D and 3D Smoothers to save content. Statistical Graphs settings for future reference. New statistical graphs in the Create Graph Wizard to visually compare datasets. These include Quantile-Quantile plots, Confidence and Prediction Ellipses, and Jitter plots.

New Statistical Analysis Features & Graphs User Interface Enhancements Transform Language Enhancements New Miscellaneous Features

• Additional numerical and graph results for • Easily add value symbols to symbol and bar • New transform language functions for • Import multiple sheets from Excel files contingency tables graphs with a new dialog available from the matrix computations including eigenvalues/ ribbon eigenvectors of real symmetric matrices, and • A notebook section can now contain one • Assumption checking and table formatting added solutions of systems of linear equations and or more subsections to correlation tests • Mini toolbar positions have been moved to regression problems. make editing objects easier • The Histogram Plus Kernel Density macro • Expanded model selection criteria in Polynomial • Restructured transform library for easier has been added to the toolbox Regression • Plots can be deleted directly from the Plot navigation panel in Graph Properties • A system memory indicator on the status • Easier access for setting population mean and • New transforms added to transform library: bar showing the amount of remaining median for one-sample • Changed the Add Axis default to be Y Axis. memory • Multilinear Orthogonal Regression and • Quantile-Quantile Plot, Confidence and Prediction • Align objects now available from the right Regression with Equality Constraints • Improved search in Help ellipses, and Jitter plots mouse menu • Passing-Bablok Regression • Greater access from a report or a result graph to the statistics interface on • Chi-Square goodness of fit test for category the ribbon data • Improvements in the random number generators when using a random seed • Statistical procedures may now use data from over 4000 worksheet columns SigmaPlot 14.5 Features GRAPHING FEATURES Multi-line Text Editor Legends • Control font, size, style, color, Greek, multiple levels • Horizontal, vertical and rectangular legend shapes of superscript and subscript, 360 degree rotation, • Simple direct labels that move with the graph 2D left, right & center justification, and line spacing • Automatic or manually created; lines and symbols • Forest Graphs (2 Types) on or off • Kernel Density (5 Types) • Legends for regressions, confidence, and prediction • Radar (5 Types) Function Plotter intervals • Automatic or manually created; lines and symbols • Dot Density (4 Types) • Place line and symbol before or after text • Vector (2 Types) on or off • Area Plots (4 Types) • Plot functions on new or existing graphs • Scatter (14 Types) Line (4 Types), Scatter and Line (10 • Plot multiple different parameter values DATA ANALYSIS FEATURES Types) simultaneously • Equation Solver: solve equations or functions • Step (8 Types) Enzyme Kinetics Module • Vertical and Horizontal Bar (4 Types), Grouped Bar containing a single independent variable and any • Analyze multiple models and rank by best fit (4 Types) number of parameters • Create multiple enzyme kinetics graphs: • Vertical and Horizontal Stacked Bar Michaelis- Menten,Lineweaver-Burk, Eadie-Hofstee, • Box (2 Types) Scatchard, Hanes- Woolf, Hill,and Dixon • Polar (3 Types) USER INTERFACE FEATURES • Contour, line and filled (2 Types) • Histograms (6 Types) • New ribbon layouts for efficient use Regression Wizard • Ternary (3 Types) • Rearrange Notebook items by dragging • 24 probability function models • Bubble, Pie, Control Charts, Needle, High-low-close, • New SigmaPlot tutorial PDF file • 7 built-in weighting functions for each model Range • Line widths from a worksheet column • Akaike information criterion computation • Quartile, Quadrant, Population • New SVG, SWF and vector PDF graphics export file • Linear and nonlinear regressions formats • 2, 3, 4, and 5-segment piecewise-linear models • Over 150 built-in, graphically-illustrated equations 3D • File import and export support is added for V13/V14 of , V9 of SAS, and V19 of SPSS • Marquardt-Levenberg algorithm with up to 50 • Multiple, intersecting plots with hidden line removal, independent variables and 500 parameters. Resizable smooth or dis- crete shading, transparent or opaque equation edit dialog which can be saved. fills, and light source shading 3D rotation, Perspective Microsoft Office Style Ribbons • Define tolerance, step size and iterations preview, Scatter, Bar, 3D line - trajectory, Mesh - with • Context sensitive feature grouping for enhanced • Define linear constraints and fix parameters to a functionality and ease of use. or without contour projections Contour, Waterfall constant value • Expanded tooltips that include hot keys. • Automatically determines your initial parameters • Alt Key Tips provide visual display of keyboard • Writes a complete statistical report to your SigmaPlot Create Graphs Easily shortcuts • Graph Style Gallery: Save any graph with all graph Notebook properties and add a bitmap image to the gallery to • Automatically graphs your results on new or existing recreate complex graphs Graph Property Redesign graphs • Graph Ribbons: select ribbon icon of the graph • New graph object and property browser. Object • Option to add 95 or 99% confidence and prediction type and style you want to create a graph or to add selection in the graph is displayed in the object bands to a results graph additional curves to an existing window and the object’s proper-ties displayed in the • Optional Reduced Chi-Square Regression weighting • Graph Wizard: easy to use, step-by-step wizard for property window. • Customize the SigmaPlot fit library or create your sing you select a graph type and pick data • Dynamic Update provides immediate graph redraw own fit functions • Default graph settings: set preferences for graph with property change. No Ok or apply button press • Generalized weight variables: predicted values, options to create favorite graphs more easily required robust regression • Templates: create custom graph page templates to • Property group styles may be copied and applied • Parameter covariance matrix and confidence store for easy accessibility and future use across like groups thus simplifying property intervals in report specification • Predicted values defined implicitly by the fit model • New window show/hide buttons for window real- Technical Axis estate management Dynamic Fit Wizard • Reciprocal (including Arrhenius), Weibull, Linear, • Solves difficult curve fitting problems by finding local Log10, Natural log, Probit, Logit, Probability, Ternary User Interface Improvements and global fit solutions percentage, unary Polar (both clockwise and counter • New tabbed window views enable quick window • Selects many initial maximally-distant starting clockwise) Category (text data automatically binned management (selection, closing, and grouping) parameter sets and ranks the resulting fits into groups) Time and date, User-defined custom • New docking panel guides providing simple • Shows the local minima with a Dynamic Fit Profile axis to create almost any scale window placement. This includes the Graph Gallery, graph Templates, Layouts, Object and Properties windows. Axis Features • Group open windows into tabbed groups Global Fit Wizard either vertically or horizontally. This collects like • Control of display, thickness, color, range, and axis • Fit multiple data sets using shared parameters offset windows for easy comparison and better window • Multiple axes: Add Axis Wizard for creating multiple management. axes easily • Object-specific mini toolbars are displayed adjacent Automatic Plot Regressions • Up to 10th order with confidence and prediction • Ticks: customize major & minor intervals, in & out to selected graph, worksheet and report objects. This intervals and regression statistics orientation, top & bottom location, length, thickness, allows rapid editing of object properties. Left mouse • Additional Plot Regression statistics color and tick mark selection from column to create selection may be turned off. custom axes • Customizable Quick Access Toolbar for frequently • Tick labels: customize prefix, suffix, numeric, and used user specific commands Column Descriptive Statistics Generated time & date Automatically • Axis breaks: customize symbol, thickness, color, Grids and Fills • Choose which statistics to display and in desired length, gap width, and post break interval • Control of color, line type, thickness, and display of order major & minor grids in X, Y, & Z direction Symbol Line and Fill Types • Control of pattern and edge color, pattern style, STATISTICS • Over 80 symbol types pattern density and edge thickness Symbols • More line types for line plots and line scatter plots, free-form lines, boxes and ellipses Reference Lines • One-way Frequency Tables • Principal Components Analysis (PCA) • 50 fill patterns for bar charts, box plots, pie charts, • Control mean, standard deviation, standard • Analysis of Covariance (ANCOVA) area plots and free-form boxes and ellipses error, user- defined constants, and 95% and 99% • Over 50 of the most frequently used statistical tests confidence intervals to analyze sci- entific data. Use data from up to 4000 • Up to 5 horizontal or vertical lines Error Bars worksheet columns • Mean, Median, First and last values for symbols • Control of color, line type, and thickness • Advisor Wizard guides you through the process of • Standard deviation, Standard error, Min and Max, choosing the appropriate statistical test First and Last entry Drop Lines • Report generation that translates the statistics into • 10th, 25th, 75th and 90% or 99% confidence • Display in any or all X, Y and Z directions plain and simple English • Calculate error bars from replicate values across • Descriptive statistics rows Antialiasing for Smooth Lines • Non-parameter tests: t-tests, ANOVA • User-defined upper and lower error bar values • Button in Format Group, Graph Page tab • Welch’s t-test assuming unequal variances • One-way, two-way and asymmetric error bars • One-way, two-way, three-way ANOVA • Percentile method: 2 types

SigmaPlot 14.5 Features • Repeated measures • Change the font type and grid colors “Picking From Column” Option • Rates and proportions • Change font for worksheet • Enter colors, patterns, symbols, line styles, tick • Regression, including Linear, Stepwise, Best Subsets, • Multiple Undo mark intervals, tick labels and more directly into Polynomial and Logistic • Format empty cells - formatted selected columns your worksheet to customize your graph the way • Correlation even if they do not contain data you want. Transforms and “picking from column” • Survival analysis (Kaplan-Meir, Cox Regression) • More flexible column titles allow for duplicates and allow you to create data dependent color gradients, numeric only titles symbols and sizes. Power and Sample Size Analysis • Enhanced data/time recognition and more formats • One sample signed rank test • Arrow-key functionality is similar to that of Microsoft Automate Routine and Complete Tasks Excel • Deming regression for errors in both x and y • Visual Basic compatible programming using built-in • Freeze Panes and print preview • Normal distribution comparison for QC analysis macro language interface • Multi-line editing - text wraps to fit the column while • Parallel line analysis • Macro recorder to save and play-back operations the row height automatically adjusts • Bland-Altman analysis for method comparison • Macro recorder to save and play-back operations • Find and replace data • Full automation object support - use Visual Basic to • Mini tool bar for worksheet cell editing create your own SigmaPlot - based applications Results Graphs From Statistics • Zoom enabled worksheet • Run built-in macros or create and add your own • Multiple graphs may be generated simultaneously • Quick worksheet window scrolling with middle scripts • Obtained from right click on statistical report or mouse wheel • Add menu commands and create dialog other Results Graph • Formatted text in worksheet cells. Text in worksheet • Export graph to PowerPoint Slide • Scatter Matrix now with 40 variables can contain super and subscripts, Greek letters, etc. • Insert Graph to Microsoft Word’ Toolbox macro • Improved default appearance of Result Graphs and this can be used in tick labels • New keyboard shortcuts in the Graph Properties and most keyboard shortcuts in the SMOOTHING Sigmaplot Notebook Manager worksheet • Holds SigmaPlot worksheets, Excel worksheets, • Macro language graph page measurement units reports, regression wizard equations, graph pages, 2D & 3D Smoothing Routines specification transforms and macros. • Macro language automatic legend state specification • Negative exponential, Running average, Loess, • Direct-editing of notebook summary information Running median, Bisquare, Inverse square, Inverse distance (for 3D smoothing) Windows Applications Sigmaplot Report Editor • Excel, Word and PowerPoint for Office 2007, Office • Insert tables with pre-defined styles or customize 2010, Office 2016 and Windows 7, TRANSFORMS completely support • Copy/Paste tabular data both ways between the • Tips and Tricks at startup SigmaPlot report and Excel worksheet • OLE 2 container and server Quick Transforms • Zoom enabled report • Use Excel worksheets inside SigmaPlot • Perform quick mathematical transforms provided in • Vertical and horizontal rulers • Right mouse button property editing a function palette • Ability to change the report background color • Selection of objects on graph page • Automatic Updating of multiple transforms in • Enhanced PDF export • Full precision and date/time Microsoft Excel copy and worksheets • Drag and Drop Word 2007/2010/2016 content paste • Improved User Interface for the Quick Transforms directly to the report • User settings are preserved from current to next dialog • Cut and paste or use OLE to combine all the SigmaPlot version important aspects of your analysis into one Mathematical Transforms document. • Set worksheet row and column titles • Choose from a wide range of styles, sizes, and colors IMPORT AND EXPORT OPTIONS • Root() and Implicit() functions from any system font. • 36 probability density and cumulative transforms • Export to most word processors Import • Histogram • Add decimal tabs, tab leader, true date/time fields • Directly import SAS data sets (.sd2 and .sas7bdat • Normalize ternary data • Auto-numbering files) Directly import Minitab data sets (.mtw & .mpj • Interpolate 3D mesh files) • Sorting Page Layout and Annotation Options • Open Axon Binary, Axon Text, ASCII Plain, Comma • Fast Fourier transforms with filters • 10 new Color Schemes and general import filter, 1-2-3 , Symphony , Quattro , • Lowess smoothing • Zoom, pan, +/- controls and zoom dialog & slider Excel, dBASE E, DIF, all • Differential equations always available • SigmaPlot files through SigmaPlot 12, SigmaStat • Data to RGB-color transform • Ctrl-3 zoom toggle between current zoom and 100% files (all versions), SYSTAT, SigmaScan Pro, Sigma • Trigonometric and algebraic functions • Drag graph with “hand” mouse cursor Scan, SigmaScan Image, Mocha • Random and Gaussian random number generation • Quick window scrolling with middle mouse wheel • Import any ODBC compliant database • Range, accumulation, precision, and more • Direct graphic file import into graph page • Run SQLqueries on tables and selectively import • Transforms are now kept in notebook files as JNB • Real-time mouse-over XY and page coordinate information files for easier organization of transforms; create feedback transform libraries; associate transforms with data • Click-through accessibility to select and edit graph Export sets objects buried under other items • SAS files (.sd2 and .sas7bdat files) • Protect and track changes to transforms for 21 CFR • OLE 2 container and server • Minitab files (.mtw & .mpj files) Part 11 • Automatic or manual legends • ASCII Text, Tabbed, Comma, 1-2-3 , Excel, DIF, all • User-defined dialog is resizable and can be saved • True WYSIWYG SigmaPlot files though SigmaPlot 12, SigmaScan Pro • Multiline text editor • PDF, PSD and HTMLexport of graphs and reports GENERAL FEATURES • Multiple curves and plots on one graph • Multiple axes on one graph Import Graphics Options • Arrange graphs with built-in templates • Load popular graphic file formats directly into Unicode • Multiple levels of zooming and custom zooming SigmaPlot graphic page • Text and symbols in different languages may be • Easily change the size and position of multiple • Import BMP, JPEG, GIF, TIFF, Cursor and Icon Files & placed on the graph selected objects simultaneously to layout and many more • Broad support for Unicode in worksheets, graphs, arrange graphs and other objects quickly and reports. • Scale graph to any size • Resize graphic elements proportionally with resizing Graph Export Options graph • Export an individual graph, a group of graphs and objects or an entire page Large, Scientific Worksheets • Alignment and position tools • Sample files in Help for graphs, statistics, macros, • Different levels of resolution and color depths: PDF, • Draw lines, ellipses, boxes, arrows with solid transforms, nonlinear regression arrowheads EPS, TIFF, JPEG, EMF, BMP, PNG • 32,000 columns by 32,000,000 rows • True color EPS vector and CMYK EPS export • Layering options • Handles numeric, text (categorical), and date & time • Compressed CMYK TIFF • Inset graphs inside one another data • Selection of graph objects • Multiple key column data sorting • Right-click property editing Publish As Web Page • Insert color, symbols, line styles and bar patterns • Color schemes • Export graphs as high-resolution Web objects • Independent graphically adjustable row height and • Paste graphic objects from other programs • WebViewer: free browser plug-in to view data used column widths • Graph page rulers to create graph or print, pan and zoom in on graph • Missing data handling • Adjustable snap-to grids without losing resolution • Data point sampling • The WebViewer supports IE 4.01 or higher. A screen • Graphical feedback of current curve and datapoint resolution JPEG file is automatically displayed for • Text support of up to 256 characters browser applications and operating systems SigmaPlot 14.5 Features Sales and Support Regional Offices

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