SigmaPlot® 11

Analyze and Graph your Data with Unparalleled Ease and Precision

Exact Graphs and Data Analysis

www.sigmaplot.com SigmaPlot® 11

The Premier Data Analysis and Scientific Graphing Package

SigmaPlot® is a scientific data analysis and graphing package with an intuitive interface and wizard technology that is designed to guide users through their analysis and graphing needs. SigmaPlot’s graphing capabilities provide the flexibility to easily customise every graph detail and create publication- quality graphs. SigmaPlot’s analytical features include advanced curve fitting capabilities and step-by-step guidance in performing over 50 frequently used statistical tests.

ROC Curve Analysis Find the exact graph for your SigmaPlot now offers Complete Module demanding research in the Advisory Statistics Methods Graph Toolbar Manage and analyse your data efficiently Determine which clinical test SigmaPlot provides more than 50 and accurately is best by creating ROC curves SigmaPlot provides more than 100 different statistical analysis method and comparing their areas using different 2D and 3D graph types so types.Users will always achieve Manipulate millions of data points in SigmaPlot’s paired and unpaired data. Even you can always find the best visual meaningful statistical results without powerful scientific data worksheet. SigmaPlot handles missing values representation of your data. having to be a statistician provides all the tools needed to analyse data, from basic statistics to advanced mathematical calculations

Create a preformatted worksheet Select the type of graph desired with possible detailed error bars and a worksheet with the correct data format Graph Style Gallery will be created. Data Save time by quickly plotting your entered into the data using previously saved work-sheet is styles or templates. immediately graphed

Global Curve Fit Analyse multiple data sets simultaneously with parameters Quickly and easily customise every Interactive Graph shared across data families. Shared parameter variability is detail of your graphs Wizard Ligand Binding Module typically reduced in this way. Double-click and edit any element to your exact SigmaPlot leads the user requirements. Create professional quality Automatically fit radioligand and step-by-step through graphs for presentations, publications or the dose response studies for multiple graph creation web with a wide range of export options like compounds with replicate data. TIFF, CMYK, PDF, HTML and more. Get More from SigmaPlot with Add-On Modules

Enzyme Kinetics Module ROC Curve Analysis Module Electrophysiology Module Ligand Binding Module The automatic choice for analyzing (Part of SigmaPlot) Directly read your electrophysiology (Part of SigmaPlot) enzyme kinetics data. Determine which clinical test data into SigmaPlot. Automatically fit radioligand and is best by creating ROC Curves This module guides you through This module saves you the time dose response studies for multiple and comparing their areas using data entry, analysis and graphing and effort of manually entering compounds with replicate data paired and unpaired data. Even of your enzyme kinetics data. data into SigmaPlot handles missing values.

Trial downloads available at www.sigmaplot.com SigmaPlot® 11

The Simplest and most Effective way to Analyze and Graph your Data

Choose from a wide range of graph types to best Create standard or asymmetric error bars. Insert present your results axis breaks and add technical detail with the SigmaPlot provides more than 100 different 2-D and scientific text editor. Paste equations, symbols, 3-D graph types. From simple 2-D scatter plots to maps and other images into your presentation. compelling contour plots, SigmaPlot gives you the exact SigmaPlot’s technical graph type you need for your demanding toolbar palettes research. With so many options, you can always find the make editing best visual representation of your data. your graphs faster than ever Instantly access SigmaPlot from Excel and toolbars are Combine two now fully powerful software customizable. packages. Launch Set properties SigmaPlot’s Graph like line thickness, Wizard directly tick lengths and from Microsoft® fonts, then apply your changes to multiple Excel® to create selections. Save properties of your favorite graphs your exact technical in the Graph Style Gallery to quickly recreate the graph. SigmaPlot’s same graphs using new data. direct access from Excel eliminates tedious cut-and Clearly present your exact scientific ideas paste data preparation steps. Compare and contrast trends in your data by Use Pre formatted Worksheets to easily creating multiple axes per graph, multiple graphs create your graphs per page and multiple pages per worksheet. Arrange Select the type multiple graphs accurately in no time using your of graph desired own page layouts with SigmaPlot’s WYSIWYG with possible layout and zoom features. detailed error Publish your SigmaPlot graphs anywhere bars and a Create stunning presentations, high-quality graphics worksheet with for journals or detailed charts for your reports with the correct data SigmaPlot’s wide range of export options. Export format will be your graphs as PDF, True CMYK EPS, TIF CMYK, created. Data JPEG, GIF, HTML, EMF, PSD and even vector EPS- entered in the CMYK - the preferred format for publication. The worksheet is immediately graphed. Submission Assistant stores an extensible list of publication requirements that is used to double Customize every element of your graphs check whether exported figures meet the SigmaPlot gives you the flexibility to customize every publication standards. The new enhanced metafile detail of your graph. Double-click on any element to format (EMF) extends the 16 bit windows metafile launch the Graph Properties box and begin editing. format (WMF) to 32 bits which provides enhanced Choose from very clearly illustrated options. Specify graph representation in the graph gallery and different colors, sizes and symbols for each data point. extended paste and paste special features.

Trial downloads available at www.sigmaplot.com SigmaPlot ® 11

Use SigmaPlot to Gain more Insight into your Data and Research

Placing SigmaPlot graphs into Microsoft You can even add your own equations to the PowerPoint slides and Microsoft Word Regression Wizard. For more complicated models documents is a breeze! with problematic data, SigmaPlot’s new Dynamic Fit Wizard searches even harder to find the best solution to your difficult curve fitting problems. The Dynamic Fit Wizard is a powerful analytical tool giving you the ability to automatically try as many initial starting conditions as necessary to assure the best fit is found. Analyze multiple data sets simultaneously with parameters shared across data families using Global Now you can incorporate clear and exact graphs in Curve Fitting. your reports and presentations. Just select the “Paste Over 130 to PowerPoint” or “Insert into MS Word” commands built-in from the Toolbox drop-down menu and your graphs equations can be fit to your data. are automatically placed in the file Manage your work efficiently Statistical Analysis is no longer a daunting task Manipulate SigmaPlot now offers almost 50 of the most millions of data frequently used statistical tests in scientific research. points in Suggestion of the most appropriate SigmaPlot’s statistical test is offered. Raw and indexed powerful data formats are accepted to avoid data scientific reformatting. Violation of data data worksheet. assumptions is checked in the With new Audit background. Reports with descriptive Trails, laboratories interpretations are generated and graphs Organize graph pages, SigmaPlot or can easily track Excel data worksheets, curve fit results, specific to each test may be created. transforms and reportseffectively in the changes to note- SigmaPlot Notebook manager books, simplifying Fit your data easily and accurately 21 CFR Part 11 compliance by logging changes. Run simple mathematical transforms effortlessly The Regression Wizard automatically determines your using SigmaPlot’s Quick Transforms feature or initial parameters, writes a statistical report, saves your create powerful mathematical routines to equation to the SigmaPlot Notebook, and adds your perform complex analysis with SigmaPlot’s results to existing graphs or creates a new one! The transform language. Elect to have Quick Regression Wizard fits nearly any equation – piecewise Transforms automatically recalculated on your continuous, multifunctional, weighted, Boolean functions worksheet. Save time by writing a transform and more – up to 10 variables and 25 parameters. once and saving it for future use. Access built-in transforms or create your own.

Trial downloads available at www.sigmaplot.com SigmaPlot® 11

The Standard for Data Analysis and Technical Graphing

Designed specifically to meet the needs of professional researchers Many scientists have struggled to create the exact technical graph to best represent their research results using spreadsheet and data analysis programs. Scientists like you designed the exact solution – SigmaPlot. It delivers a full range of graphing options and over 100 2-D and 3-D graph types. And flexible editing options give you more control over the look and design of your graph than other graphing packages Analyze your data quickly and easily SigmaPlot provides all the fundamental tools you need to analyze your data - from basic statistics to advanced mathematical calculations. Run one of the many statistical tests with ease, fit a curve or plot a function and get a detailed report of the results in seconds. Apply built-in transforms to perform calculations in one easy step and create special graph types. Solve equations or functions containing a single independent variable and any number of functions. With SigmaPlot it’s all so simple!

Share SigmaPlot graphs on the Internet The SigmaPlot WebViewer allows you to automatically generate active Web pages from your graphs or embed the objects within other Web pages. Viewers can explore the data used in your graphs and zoom, pan or print images at full resolution directly from their Web browser.

Save time and effort by automating complex and repetitive tasks

Create macros in no time with SigmaPlot’s easy-to-use VBA-based I’ve owned and used SigmaPlot macro language. Not a programmer? No problem - record macros by for more than a decade and I love it. point and click with the macro recorder. Use macros to acquire your We do both clinical and data, execute powerful analytical methods and create industry-specific pharmacological research and SigmaPlot enables us to analyze and or field-specific graphs. Use built-in macros as provided or use these sort through large amounts of data macros as a base to create your own macros. Share the power of to create and publish graphs that SigmaPlot with less experienced users by tailoring the SigmaPlot can be transposed to manuscripts interface to your application with macros. Create custom dialog boxes, and directly submitted to publications menu choices and forms to help guide novice users through a session. - the product is that user friendly and great! I can’t wait to upgrade to Tap into SigmaPlot’s capabilities from external sources that have Visual SigmaPlot 11. Basic embedded, including Microsoft Word and Excel or custom software - Carmen Cuffari, applications. Run a macro script in Microsoft Word or Excel that calls Pediatric Gastroenterologist, on SigmaPlot to access data, generate a graph and embed your report. Baltimore, MD With SigmaPlot’s OLE automation, you get unlimited flexibility.

Trial downloads available at www.sigmaplot.com SigmaPlot 11 Features Error bars SMOOTHING "Picking from Column" Option • Mean, Median, First and Last values for symbols 2D & 3D Smoothing Routines • Enter colours, patterns, symbols, line styles, tick mark • Standard deviation, Standard error intervals, tick labels and more directly into your worksheet GRAPHING FEATURES • Negative exponential • 10th, 25th, 75th, and 90th Percentile • Running average to customise your graph the way you want. Transforms 2D • Min and Max • Loess and "picking from column" allow you to create data • Vector - 2 Types * • 95% or 99% confidence • Running median dependent colour gradients, symbols and sizes. • Area Plots - 4 Types • Calculate error bars from replicate values across rows • Bisquare Automate Routine and Complex Tasks • Scatter - 14 types • User-defined upper and lower error bar values • Inverse square • Visual Basic compatible programming using built-in • Line - 4 types • One way, two way and asymmetric error bars • Inverse distance (for 3D smoothing) macro language interface • Scatter and Line - 10 types • Percentile method: 2-types • Macro recorder to save and play-back operations • Step - 8 types TRANSFORMATIONS Multiline Text Editor • Full automation object support - use Visual Basic to • Vertical Bar - 2 types, Vertical Grouped Bar - 2 types • Control font, size, style, colour, Greek, multiple levels of Quick Transforms create your own SigmaPlot-based applications • Horizontal Bar - 2 types, Horizontal Grouped Bar - 2 types superscript & subscript, 360 degree rotation, left, right • Perform quick mathematical transforms provided in a • Run built-in macros or create and add your own scripts • Vertical, Stacked Bar & center justification, and line spacing function palette • Add menu commands and create dialog boxes • Horizontal, Stacked Bar • Automatic Updating of multiple transforms in worksheets * • Toolbox menu: helpful macros appear as a separate • Box - 2 types Grids and Fills Mathematical Transforms menu item • Polar - 3 types • Control of colour, line type, thickness, and display of major & • Export graph to PowerPoint Slide • Contour, line and filled - 2 types minor grids in X, Y, & Z direction • 36 probability density and cumulative transforms * • Histogram • New 'Insert Graph to Microsoft Word' Toolbox macro • Histograms - 6 types • Control of pattern and edge colour, pattern style, pattern • New keyboard shortcuts in the Graph Properties and most • Ternary - 3 types density, and edge thickness Symbols • Normalise ternary data • Interpolate 3D mesh keyboard shortcuts in the worksheet • Bubble Reference Lines • Pie • Sorting Windows Application • Control mean, standard deviation, standard error, user-defined • Fast Fourier transforms with filters • Excel, Word and PowerPoint for Office 2000 and • Control Charts constants, 95% and 99% confidence intervals • Needle • Lowess smoothing support ToolTips • Up to 5 horizontal or vertical lines • Differential equations • Tips and Tricks at startup • High-low-close, Range, Quartile • Control of colour, line type, and thickness • Quadrant • Data to RGB-colour transform • Full 32-bit implementation • Population Drop lines • Trigonometric and algebraic functions • OLE 2 container and server • Display in any or all X, Y, and Z directions • Random and Gaussian random number generation • Use Excel worksheets inside SigmaPlot 3D • Range, accumulation, precision, and more • Controls have bitmaps to give feedback about selections • Select any object to make precise modifications * Legends • Transforms are now kept in notebook files as JNB files for • Right mouse button property editing • Automatically interpolate unordered 3D data • Automatic or manually created easier organisation of transforms; create transform libraries; • Selection of objects on graph page • Multiple, intersecting plots with hidden line removal, • Legends for regressions, confidence, and prediction intervals associate Transforms with data sets • Full precision and date/time Microsoft Excel copy and paste smooth or discrete shading, transparent or opaque fills, • Turn on and off lines and symbols • Protect and track changes to transforms for 21 CFR Part 11 and light source shading • Place line and symbol before or after text Microsoft Office Integration • 3D rotation • You can open Excel spreadsheets directly inside Function Plotter GENERAL FEATURES SigmaPlot, allowing you to use the many features • Perspective preview • Plot 2D and 3D functions • Scatter Large, Scientific Worksheets Excel offers. Use in-cell formulas and other Excel data • Over 100 2D and 3D built-in, graphically-illustrated analysis tools on your data. • Bar equations • 32,000 columns by 32,000,000 rows • 3D line - trajectory • Handles numeric, text (categorical), and date & time data • One-click access to directly launch SigmaPlot from • User-defined parameters, scale and range Microsoft Excel • Mesh - with or without contour projections • Customise the SigmaPlot library of functions or create your own • Data sorting • Contour • Plot functions on new or existing graphs • Rename, insert and delete rows and columns IMPORT & EXPORT OPTIONS • Waterfall • Plot multiple different parameter values simultaneously • Insert colour, symbols, line styles and bar patterns Import Create Graphs Easily • Select line properties for each function • Independent graphically adjustable row height and • Directly import SAS data sets (.sd2 and .sas7bdat files) • Pre-formatted Worksheets: Use graph and worksheet dis • Equation Solver: solve equations or functions containing a column widths • Directly import Minitab data sets (.mtw & .mpj files) plays to design the graph. Entering new data immediately single independent variable and any number of parameters • Missing data handling • Open Axon Binary, Axon Text, ASCII Plain, Comma and displays the results * • Data point sampling general import filter, 1-2-3T, Symphony T, Quattro T, Excel, Graphs Created Through Transforms • Program Startup Screen: helps both first time users or • Graphical feedback of current curve and datapoint dBASE E, DIF, all SigmaPlot files through SigmaPlot 9.01, • Gaussian cumulative distribution power users start their workflow with easy access to previously • Text support of up to 256 characters SigmaStat files (all versions), SYSTAT, SigmaScan Pro, • Area - shading under and between curves used documents, quick Excel and Access database import; • Change the font type and grid colours Sigma Scan, SigmaScan Image, Mocha • Z plane design use older documents as templates for new work • Change font for worksheet • Import any ODBC compliant database • Kaplan-Meier survival • Graph Style Gallery: Save any graph with all graph • Multiple Undo • Run SQL queries on tables and selectively import • Frequency properties and add a bitmap image to the gallery to recreate • Format empty cells - formatted selected columns even if they information • Complex Control charts complex graphs do not contain data Export • Graph Toolbar: select toolbar icon of the graph type and style DATA ANALYSIS FEATURES • More flexible column titles allow for duplicates and • SAS files (.sd2 and .sas7bdat files) you want to create a graph or to add additional curves to an numeric only titles • Minitab files (.mtw & .mpj files) Regression Wizard existing graph • Enhanced data/time recognition and more formats • ASCII Text, Tabbed, Comma, 1-2-3T, Excel, DIF, all • Linear and nonlinear regressions • Graph Wizard: easy to use, step-by-step wizard for helping • Arrow-key functionality is similar to that of Microsoft Excel SigmaPlot files though SigmaPlot 9.01, SigmaScan Pro • 2, 3, 4, and 5-segment piecewise-linear models you select a graph type and pick data • Freeze Panes and print preview • PDF, PSD and HTML export of graphs and reports • Over 100 built-in, graphically-illustrated equations • Default graph settings: set preferences for graph options to • Multi-line editing - text wraps to fit the column while the • Marquardt-Levenberg algorithm with up to 10 Import Graphics Options create favorite graphs more easily row height automatically adjusts independent variables and 25 parameters • Load popular graphic file formats directly into SigmaPlot • Templates: create custom graph page templates to store for • Find and replace data • Define constraints, tolerance, step size and iterations graphic page easy accessibility and future use • Automatically determines your initial parameters SigmaPlot Notebook Manager • Import BMP, JPEG, GIF, TIFF, Cursor and Icon Files & many more • "Intersections" now supported for area fills • Writes a complete statistical report to your SigmaPlot Notebook • Dialog-bar-based notebook manager has several states: • Automatically graphs your results on new or existing graphs docks, resizable, hide-able, summary information mode, Graph Publication Export Options Technical Axes • Option to add 95 or 99% confidence and prediction bands to a etc. • Submission Assistant stores an extensible list of • Reciprocal (including Arrhenius) results graph • Can hold SigmaPlot worksheets, Excel worksheets, publication requirements and is used to double check • Weibull • Optional Reduced Chi-Square Regression weighting reports, regression wizard equations, graph whether exported figures meet standards of publication • Linear • Customise the SigmaPlot fit library or create your own fit pages, transforms and macros. and allows creation of customised output profiles • Log10 functions • Direct-editing of notebook summary information • Export an individual graph, a group of graphs and objects, • Natural log SigmaPlot Report Editor or an entire page • Probit Dynamic Fit Wizard • Cut and paste or use OLE to combine all the important aspects • Different levels of resolution and colour depths: PDF, EPS, • Logit • Solves difficult curve fitting problems by finding local and global of your analysis into one document. Choose from a wide TIFF, JPEG, EMF, BMP • Probability fit solutions range of styles, sizes, and colours from any system font. • True colour EPS vector and CMYK EPS export • Reverse • Selects many initial maximally-distant starting parameter sets • Export to most word processors • Compressed CMYK TIFF • Ternary percentage and ranks the resulting fits • Add decimal tabs, tab leader, true date/time fields • Publication Help: guides user through the complexities • Ternary unary • Shows the local minima with a Dynamic Fit Profile graph • Auto-numbering selecting the correct DPI, image size, file export format • Polar (both clockwise and counter clockwise) • Improved formatting ruler Publish as Web Page • Category (text data automatically binned into groups) Global Fit Wizard * Page Layout and Annotation Options • Export graphs as high-resolution Web objects • Time and date • Fit multiple data sets using shared parameters * • Direct graphic file import into graph page • WebViewer: free browser plug-in to view data used to create • User-defined custom axis to create almost any scale • Real-time mouse-over XY and page coordinate feedback graph or print, pan and zoom in on graph without Axis Features Automatic Linear Regressions • Click-through accessibility to select and edit graph losing resolution • Control of display, thickness, colour, range, and axis breaks • Up to 10th order with confidence and prediction intervals objects buried under other items • The WebViewer supports IE 4.01 or higher. A screen resolution • Offset axes and regression statistics • OLE 2 container and server JPEG file is automatically displayed for browser applications • Automatic titles • Automatic or manual legends and operating systems Column Descriptive Statistics Generated • Multiple axes: Add Axis Wizard for creating multiple axes easily • True WYSIWYG Automatically • Ticks: customize major & minor intervals, in & out orientation, • Multiline text editor SYSTEM REQUIREMENTS top & bottom location, length, thickness, colour, and tick mark • Multiple curves and plots on one graph selection from column to create custom axes STATISTICS • Multiple axes on one graph • Windows Vista: • Labels: customise prefix, suffix, numeric, and time & date • Over 50 of the most frequently used statistical tests to • Arrange graphs with built-in templates - 1 GHz 32-bit (x86) or 64-bit (x64) processor • Axis breaks: customize symbol, thickness, colour, length, analyse scientific data * • Multiple levels of zooming and custom zooming - 1 GB of system memory gap width, and post break interval • Easily change the size and position of multiple selected • Advisor Wizard guides you through the process of choosing - 100 MB of available hard disk space * objects simultaneously to lay out and arrange graphs Symbol Types the appropriate statistical test - CD-ROM drive • Report generation that translates the statistics into plain and and other objects quickly • Over 80 symbol types - 800x600 SVGA/256 colour display or better • More line types for line plots and line scatter plots, simple English * • Scale graph to any size free-form lines, boxes and ellipses • Descriptive statistics * • Resize graphic elements proportionally with resizing graph - Internet Explorer 6 or higher • 50 fill patterns for bar charts, box plots, pie charts, • Non-parameter tests: t-tests, ANOVA * • Alignment and position tools * • Draw lines, ellipses, boxes, arrows area plots and free-form boxes and ellipses • One-way, two-way, three-way ANOVA • Windows XP and below: • Edit font when using text as symbol • Repeated measures * • Layering options • Over 16 million custom colours • Access new lines, fills, and symbols directly from graph prop- • Rates and proportions * - 300 megahertz (MHz) processor, erties dialog, toolbar, legend page, and the symbol dialog box • Inset graphs inside one another - 256 MB of system memory, • Correlation * • Selection of graph objects - 100 MB of available hard disk space • Survival analysis (Kaplan-Meir, Cox Regression) * • Right-click property editing • Power and sample size analysis * • Colour schemes - CD-ROM drive, • Paste graphic objects from other programs - 800x600 SVGA/256 colour display or better * New Features added in SigmaPlot 11 • Graph page rulers - Internet Explorer 6 or higher • Adjustable snap-to grids

For more information, visit us at www.sigmaplot.com Exact Graphs and Data Analysis

North, Central & South Germany and Austria: UK and Ireland: India: Australia and New Zealand : America: Systat Software GmbH Systat Software, Inc. Cranes Software International Ltd. Cranes Software International Pty Ltd. Systat Software, Inc. Schimmelbuschstr 25 UK Branch Office No 52, Bannerghatta Road, Level 6, College House 1735 Technology Drive, Suite 430 D-40699 Erkrath Germany 24, Vista Centre, 50 Salisbury Road, BTM Layout, I Stage, I Phase, 552 Lonsdale Street, Melbourne, 3000 San Jose, CA 95110 USA Phone: +49.2104.9540 Hounslow, - TW4 6JQ, London, UK. Bangalore, 560076, Karnataka, India Phone: 800-797-7401 Fax: +49.2104.95410 Australia +44-(0)208-538 0128 Fax: 800-797-7406 E-mail: [email protected] Phone: Phone: +91-80-4128 1111 Phone: +61 3 9670 8997 Email: [email protected] Fax: + 44-(0)208-538 0273 Fax: +91-80-4128 0203 Fax: +61 3 9602 5050 Email: [email protected] Email: [email protected] Email: [email protected]

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