Exact Graphs for Exact Science SigmaPlot 10 SigmaPlot® 10 Designed Specifically to Meet the Needs of the Professional Researcher SigmaPlot is the scientific graphing and data analysis package with an award-winning interface and intuitive wizard technology that guides users step-by-step through the graph creation and data analysis process. SigmaPlot provides the flexibility to easily customize every graphic detail and create publication-quality graphs you simply can’t get from a basic spreadsheet package. Join the more than 100,000 scientists and engineers who have used SigmaPlot to show meaningful discoveries in their research data for technical publications,presentations or the web. The Most Effective Way to Analyze and Graph Research Data Find the exact graph for your demanding research Manage and analyze your data efficiently and accurately SigmaPlot provides more than 80 different 2D and 3D graph types. Manipulate millions of data points in SigmaPlot's powerful scientific data With so many options, you can always find the best visual worksheet. SigmaPlot provides all the fundamental tools needed to analyze representation of your data. data, from basic statistics to advanced mathematical calculations.

Graph Style Gallery Customize every Save time by quickly plotting element of your graphs your data using previously SigmaPlot gives you the saved styles or templates. flexibility to customize every detail of your graph by double-clicking and The Regression Wizard editing any element to guides you through the your exact specifications, curve fitting process even if it is buried Dynamic Fit Wizard compli- under other elements. ments the Regression Wizard by automatically searching Publish & Share even harder to find the best solution to your most difficult Your Work Anywhere curve fitting problems. Create professional-quality graphs for presentations, publications, or the web with Interactive Graph Wizard a wide range of export options. Leads you step-by-step through graph creation.

GET MORE POWER FROM SIGMAPLOT WITH ADD-ON MODULES SigmaStat 3.5 Enzyme Kinetics Module ROC Curve Analysis Module Ligand Binding Module Advisory Statistical Software The automatic choice for Determine which clinical test is Quickly analyze and plot ligand/ This easy-to-use statistical software analyzing enzyme kinetics data best by creating ROC Curves and receptor and dose response data package guides users through every This module guides you through comparing their areas using paired Automatically fit radioligand and step of the analysis, allowing them data entry, analysis and graphing and unpaired data. Even handles dose response equations for missing values. to perform powerful statistical of your enzyme kinetics data. multiple compounds with replicate analysis without being a statistical Select the study type in the Electrophysiology Module data. Select the type of study in expert. Access SigmaStat’s expert customized Data Entry Wizard, Directly read your electrophysiology the customized dialog and specify choose from 56 built-in equations, data into SigmaPlot the number of replicate values to statistical analysis within SigmaPlot’s then discover the best fit to instantly plot and analyze your Statistics menu. SigmaStat is characterize the reaction mechanism. The Electrophysiology Module data. All graphs and analysis tailored to the areas of life science Automatically display a series of provides a preview of your data appear as notebook items, to and is offered as a stand alone graphs to quickly determine the so you can select and import the efficiently manage your work. product or a powerful companion type of inhibition. relevant information for your to SigmaPlot. analysis. The Technical Graphing Standard SigmaPlot 10 Create Your Exact Technical Graph and Publish Your Work Anywhere

Choose from a wide range of graph types Share your SigmaPlot graphs with just to best present your results about anyone, anywhere SigmaPlot provides more than 80 different 2-D and 3-D Create stunning presentations, high-quality graphics graph types. From simple 2-D scatter plots to compelling for journals or detailed charts for your reports with contour plots, SigmaPlot gives you the exact technical SigmaPlot’s wide range of export options. Export your graph type you need for your demanding research. graphs as PDF, True CMYK EPS, TIF CMYK, JPEG, With so many options, you can always find the best HTML and even vector EPS-CMYK - the preferred format visual representation of your data. for publication. Incorporate your graphs into Microsoft Word documents or Microsoft PowerPoint presentations Customize every element of your graphs with one simple step. SigmaPlot gives you the flexibility to customize every Instantly access SigmaPlot from Excel detail of your graph. Double-click on any element to launch the Graph Properties box and begin editing. Combine two powerful Choose from unmistakably clear, illustrated options. software packages. Specify different colors, sizes and symbols for each data Launch SigmaPlot’s point. Create standard or asymmetric error bars. Insert Graph Wizard directly axis breaks and add technical detail with the scientific text from Microsoft® editor. Paste equations, symbols, maps and other images into Excel® to create your your presentation. exact technical graph. SigmaPlot’s direct SigmaPlot’s toolbar access from Excel palettes make editing eliminates tedious Launch the SigmaPlot Graph Wizard from your graphs faster ’s toolbar or menu, then cut-and-paste data select the row and column references in than ever. Set Excel worksheet format. preparation steps. properties like line thickness, tick Placing SigmaPlot graphs into Microsoft lengths and fonts, PowerPoint slides and Microsoft Word then apply your documents is a breeze! changes to multiple selections. Save Placing your SigmaPlot graphs into Microsoft® PowerPoint® properties of your slides and Microsoft® Word® documents is simple. Now favorite graphs in the you can incorporate clear and exact graphs in your reports Graph Style Gallery and presentations. Just select the “Paste to PowerPoint” to quickly recreate or “Insert into MS Word” commands from the Toolbox drop-down menu and your graphs are automatically placed the same graphs With so many choices for customizing the using new data. plot area, SigmaPlot is flexible enough to in the file. meet the needs of almost any researcher.

Clearly present your exact scientific ideas Compare and contrast trends in your data by creating multiple axes per graph, multiple graphs per page and multiple pages per worksheet. Arrange multiple graphs accurately in no time using your own page layouts with

SigmaPlot’s WYSIWYG layout and zoom features. Edit graphs to your exact specifications and easily place them in Microsoft PowerPoint presentations. Exact Graphs for Exact Science SigmaPlot 10

Our lab studies the visual signaling pathway in retinal rod and cone photoreceptors. We use Sigmaplot exclusively for all of our data analysis needs. It has the power to carry out complex curve fitting routines to model the experimental results in terms of the underlying biochemical reaction mechanism. Sigmaplot provides our lab a user-friendly path for creating visual representations of our results, and allows us to easily prepare figures that are compatible with electronic publication in scientific journals. - Rick H. Cote, Ph.D “ Professor of Biochemistry and Molecular Biology, University New Hampshire Fit your data easily and accurately Manage your work efficiently ” Fit your data easily and accurately with the SigmaPlot Manipulate millions of data points in SigmaPlot’s powerful Regression Wizard. The Regression Wizard automatically scientific data worksheet. Organize graph pages, SigmaPlot determines your initial parameters, writes a statistical or Microsoft Excel data worksheets, curve fit results and report, saves your equation to the SigmaPlot Notebook, reports effectively in the SigmaPlot Notebook Manager. and adds your results to existing graphs or creates a new one! Run simple mathematical transforms effortlessly using SigmaPlot’s quick transforms feature or create powerful mathematical routines to perform complex analyses with SigmaPlot’s transform language. Save time by writing a transform once Fit your data with a few mouse clicks as the wizard guides you through the curve fitting process. and saving it for future use. Access With a number of new worksheet improvements The Regression Wizard fits nearly any equation - piecewise and the new Notebook Manager, you will instantly built-in transforms witness increased workflow efficiency in your continuous, multifunctional, weighted, Boolean functions or create your own. research and analysis. and more - up to 10 variables and 25 parameters. You can even add your own equations to the Regression Wizard. Save time and effort by automating complex and repetitive tasks For more complicated models with problematic data, SigmaPlot’s new Dynamic Fit Wizard searches even harder Create macros in no time with SigmaPlot’s easy-to-use to find the best solution to your difficult curve fitting VBA-based macro language. Not a programmer? problems. The Dynamic Fit Wizard is a powerful analytical No problem - record macros by point and click with the tool to solve curve fitting problems that have multiple macro recorder. Use macros to acquire your data, execute local minima even when inaccurate initial parameter powerful analytical methods and create industry- specific estimates are supplied. It works by giving you the ability or field-specific graphs. Use built-in macros as provided to automatically try as many initial starting conditions as or use these macros as a base to create your own macros. necessary to assure the best fit is found. Share the power of SigmaPlot with less experienced users Plot any mathematical function by tailoring the SigmaPlot interface to your application with macros. Create custom dialog boxes, menu choices Use the Function and forms to help guide novice users through a session. Plotter to plot user-defined and Tap into SigmaPlot’s capabilities from external sources parameterized that have Visual Basic embedded, including Microsoft equations with Word and Excel or custom software applications. Run a single mouse a macro script in Microsoft Word or Excel that calls on click. Just type SigmaPlot to access data, generate a graph and embed the function or your report. With SigmaPlot’s OLE automation, you select one from the built-in library and specify the param- get unlimited flexibility. eters and the range. It’s that easy! Create your own built-in functions and save for future use. Plot functions on new or existing graphs or plot multiple functions simultaneously using different parameter values. SigmaPlot 10 Features Error bars TRANSFORMATIONS Automate Routine and Complex Tasks • Mean, Median, First and Last values for symbols Quick Transforms • Visual Basic compatible programming using built-in • Standard deviation, Standard error macro language interface GRAPHING FEATURES • Perform quick mathematical transforms provided in a • 10th, 25th, 75th, and 90th Percentiles function palette • Macro recorder to save and play-back operations 2D • Min and Max • Full automation object support - use Visual Basic to • Area Plots - 4 Types • 95% or 99% confidence Mathematical Transforms create your own SigmaPlot-based applications • Scatter - 14 types • Calculate error bars from replicate values across rows • 12 New Probability Transforms* • Run built-in macros or create and add your own scripts • Line - 4 types • User-defined upper and lower error bar values • Histogram • Add menu commands and create dialog boxes • Scatter and Line - 10 types • One way, two way and asymmetric error bars • Normalize ternary data • Toolbox menu: helpful macros appear as a separate • Step - 8 types • Percentile method: choose between nearest integer • Interpolate 3D mesh menu item • Vertical Bar - 2 types (graphical) or value dependent (numerical) algorithms • Sorting • Export graph to PowerPoint Slide (macro) • Horizontal Bar - 2 types • Fast fourier transforms with filters • New 'Insert Graph to Microsoft Word' Toolbox macro Multiline Text Editor • Vertical, Grouped Bar - 2 types • Lowess smoothing • New keyboard shortcuts in the Graph Properties and most • Control font, size, style, color, Greek, multiple levels of • Horizontal, Grouped Bar - 2 types • Differential equations Microsoft Excel keyboard shortcuts in the worksheet superscript & subscript, 360 degree rotation, left, right • Vertical, Stacked Bar • Data to RGB-color transform & center justification, and line spacing Windows Application • Horizontal, Stacked Bar • Trigonometric and algebraic functions • Statistics including 95% and 99% confidence, t-tests • Excel, Word and PowerPoint for Office 2000 and • Box - 2 types Grids and Fills support ToolTips • Polar - 3 types • Control of color, line type, thickness, and display of major & • Random and gaussian random number generation • Range, accumulation, precision, and more • Tips and Tricks at startup • Contour, line and filled - 2 types minor grids in X, Y, & Z direction • Full 32-bit implementation • Histograms - 6 types • Control of pattern and edge color, pattern style, pattern • Transforms are now kept in notebook files as JNB files for easier organization of transforms; create transform libraries; • OLE 2 container and server • Ternary - 3 types Time-Series density, and edge thickness Symbols • Use Excel worksheets inside SigmaPlot • Bubble associate Transforms with data sets* Reference Lines • Protect and track changes to transforms for 21 CFR Part 11* • Uninstaller • Pie • Control mean, standard deviation, standard error, user-defined • Controls have bitmaps to give feedback about selections • Control Charts constants, 95% and 99% confidence intervals GENERAL FEATURES • Right mouse button property editing • Needle • Up to 5 horizontal or vertical lines Large, Scientific Worksheets • Selection of objects on graph page • High-low-close, Range, Quartile • Control of color, line type, and thickness • Full precision and date/time Microsoft Excel copy and paste • Quadrant • Up to 32,000 columns by millions of rows • Population Drop lines • Handles numeric, text (categorical), and date & time data Microsoft Office Integration • Display in any or all X, Y, and Z directions • Automatically generate column statistics • You can open Excel spreadsheets directly inside 3D • Data sorting SigmaPlot, allowing you to use the many features • Automatically interpolate and unordered 3D data* Legends • Rename, insert and delete rows and columns Excel offers. Use in-cell formulas and other Excel data • Multiple, intersecting plots with hidden line removal, • Automatic or manually created • Insert color, symbols, line styles and bar patterns analysis tools on your data. smooth or discrete shading, transparent or opaque fills, • Legends for regressions, confidence, and prediction intervals • Independent graphically adjustable row height and • One-click access to directly launch SigmaPlot from and light source shading • Turn on and off lines and symbols column widths Microsoft Excel • 3D rotation • Place line and symbol before or after text • Missing data handling • Perspective preview SigmaStat 3.5 Integration* Function Plotter • Data point sampling • SigmaStat's statistical capabilities are directly accessible • Scatter • Plot 2D and 3D functions • Graphical feedback of current curve and datapoint • Bar in SigmaPlot through the statistics menu. • Over 100 2D and 3D built-in, graphically-illustrated • Text support of up to 256 characters • Over 30 of the most frequently used statistical tests to • 3D line - trajectory equations • Change the font type and grid colors • Mesh - with or without contour projections* analyze scientific research • User-defined parameters, scale and range • Change font for worksheet • Advisor Wizard guides you through the process of • Contour • Customize the SigmaPlot library of functions or create your own • Multiple Undo • Waterfall choosing the appropriate statistical test • Plot functions on new or existing graphs • Format empty cells - formatted selected columns even if they • Report generation that translates the statistics into plain Create Graphs Easily • Plot multiple different parameter values simultaneously do not contain data and simple English • NEW Program Startup Screen: helps both first time users or • Select line properties for each function • More flexible column titles allow for duplicates and • Descriptive statistics power users start their workflow with easy access to previously • Equation Solver: solve equations or functions containing a numeric only titles • Non-parametric tests: t-tests, ANOVA used documents, quick Excel and Access database import; single independent variable and any number of parameters • Enhanced data/time recognition and more formats • One-way, two-way, three-way ANOVA use older documents as templates for new work* • Arrow-key functionality is similar to that of Microsoft Excel • Repeated measures • Graph Style Gallery: Save any graph with all graph Graphs Created Through Transforms • Freeze Panes and print preview • Gaussian cumulative distribution • Rates and proportions properties and add a bitmap image to the gallery to recreate • Multi-line editing - text wraps to fit the column while the • Area - shading under and between curves • Correlation complex graphs row height automatically adjusts • Z plane design • Survival analysis (Kaplan-Meir) • Graph Toolbar: select toolbar icon of the graph type and style • Find and replace data • Vector • Power and sample size analysis you want to create a graph or to add additional curves to an • Kaplan-Meier survival SigmaPlot Notebook Manager existing graph IMPORT & EXPORT OPTIONS • Frequency • Can hold SigmaPlot worksheets, Excel worksheets, • Graph Wizard: easy to use, step-by-step wizard for helping • Complex Control charts reports, documents, regression wizard equations, graph Import you select a graph type and pick data pages, transforms and macros. • Directly import SAS data sets (.sd2 and .sas7bdat files)* • Default graph settings: set preferences for graph options to DATA ANALYSIS FEATURES • New dialog-bar-based notebook that has several states: • Directly import Mintab data sets (.mtw & .mpj files)* create favorite graphs more easily Regression Wizard docks, resizable, hide-able, summary information mode, etc. • Axon Binary, Axon Text, ASCII Plain, Comma and general • Templates: create custom graph page templates to store for • Linear and nonlinear regressions • Direct-editing of notebook summary information import filter, 1-2-3T, Symphony T, Quattro T, Excel, easy accessibility and future use • New piecewise-linear models for regression include 2, 3, 4, dBASE E, DIF, all SigmaPlot files through SigmaPlot 8.02, • "Intersections" now supported for area fills SigmaPlot Report Editor and 5-segment piecewise-linear models with automatic SigmaStat files (all versions), SYSTAT, SigmaScan Pro, • Improved Graph Wizard: retains last settings, you can click • Cut and paste or use OLE to combine all the important aspects parameter estimation* Sigma Scan, SigmaScan Image, Mocha 'Finish Early,' Gallery Graphs are listed of your analysis into one document. Choose from a wide • Over 100 built-in, graphically-illustrated equations range of styles, sizes, and colors from any system font. • Import any ODBC compliant database Technical Axes • Marquardt-Levenberg algorithm with up to 10 • Export to most word processors • Run SQL queries on tables and selectively import • Reciprocal (including Arrhenius)* independent variables and 25 parameters • Add decimal tabs, tab leader, true date/time fields information • Weibul* • Define constraints, tolerance, step size and iterations • Auto-numbering Export • Linear • Automatically determines your initial parameters • Improved formatting ruler • SAS files (.sd2 and .sas7bdat files)* • Log10 • Writes a complete statistical report to your SigmaPlot Notebook • Minitab files (.mtw & .mpj files)* Page Layout and Annotation Options • Natural log • Automatically graphs your results on new or existing graphs • ASCII Text, Tabbed, Comma, 1-2-3T, Excel, DIF, all • Direct graphic file import into graph page* • Probit • Option to add 99% confidence and prediction bands to a SigmaPlot files though SigmaPlot 9.01, SigmaScan Pro • Real-time mouse-over XY and page coordinate feedback* • Logit results graph* • PDF and HTML export of graphs and reports • Click-through accessibility to select and edit graph • Probability • Optional Chi-Square Regression Weighting* objects buried under other items* Import Graphics Options* • Reverse • Edit code so you can customize the SigmaPlot library of • OLE 2 container and server • Load popular graphic file formats directly into SigmaPlot • Ternary percentage functions or create your own • Automatic or manual legends graphic page • Ternary unary • Specify the range for the predicted values output by curve-fitter • Import BMP, JPEG, GIF, TIFF, Cursor and Icon Files & many more • Polar (both clockwise and counter clockwise)* • True WYSIWYG • Category (text data automatically binned into groups) Dynamic Fit Wizard* • Multiline text editor Graph Publication Export Options • Time and date • Compliments the Regression Wizard by providing • Multiple curves and plots on one graph • New Submission Assistant Stores an extensible list of • User-defined custom axis to create almost any scale hundreds of initial parameter estimates to minimize the • Multiple axes on one graph publication requirements and is used to double check squared residual errors, produce individual curve fits for • Arrange graphs with built-in templates whether exported figures meet standards of publication Axis Features each, and chose the best in a matter of seconds • Multiple levels of zooming and custom zooming and allows creation of customized output profiles* • Control of display, thickness, color, range, and axis breaks • Achieves the best fit by automatically choosing and trying • Easily change the size and position of multiple selected • Export an individual graph, a group of graphs and objects, • Offset axes numerous initial parameter estimates to find the global minimum, objects simultaneously to lay out and arrange graphs or an entire page • Automatic titles even if you supply a complicated model with problematic data and other objects quickly* • Different levels of resolution and color depths: PDF,EPS, • Multiple axes: Add Axis Wizard for creating multiple axes easily • The initial fitting parameters can be generated automatically or • Scale graph to any size TIFF, JPEG, WMF, BMP • Ticks: customize major & minor intervals, in & out orientation, can be user specified • Resize graphic elements proportionally with resizing graph • True color EPS vector and CMYK EPS export top & bottom location, length, thickness, color, and tick mark • Alignment and position tools • Compressed CMYK TIFF selection from column to create custom axes Automatic Linear Regressions • Up to 10th order with confidence and prediction intervals • Draw lines, ellipses, boxes, arrows • Publication Help: guides user through the complexities • Labels: customize prefix, suffix, numeric, and time & date • Layering options selecting the correct DPI, image size, file export format • Axis breaks: customize symbol, thickness, color, length, and regression statistics • Over 16 million custom colors Publish as Web Page gap width, and post break interval Column Statistics Generated Automatically • Inset graphs inside one another • Export graphs as high-resolution Web objects • Size, sum, mean, minimum, maximum, standard deviation, Symbol Types • Selection of graph objects • WebViewer: free browser plug-in to view data used to create standard error, skewness, minimum positive, number of • Over 80 symbol types • Right-click property editing graph or print, pan and zoom in on graph without missing values, and 95% & 99% confidence intervals • More line types types for line plots and line scatter plots, • Color schemes losing resolution free-form lines, boxes and ellipses* • Paste graphic objects from other programs SMOOTHING • The WebViewer supports IE 4.01 or higher. A screen resolution • More fill patterns for bar charts, box plots, pie charts, • Graph page rulers JPEG file is automatically displayed for browser applications 2D & 3D Smoothing Routines area plots and free-form boxes and ellipses* • Adjustable snap-to grids and operating systems • Negative exponential • Edit font when using text as symbol "Picking from Column" Option • Running average • Access new lines, fills, and symbols directly from graph prop- • Enter colors, patterns, symbols, line styles, tick mark SYSTEM REQUIREMENTS • Loess erties dialog, toolbar, legend page, and the symbol dialog box intervals, tick labels and more directly into your worksheet • Windows XP, 2000 or NT 4.0 • Running median to customize your graph the way you want. Transforms • Pentium 200 or clone • Bisquare and "picking from column" allow you to create data • 64MB or more of RAM • Inverse square dependent color gradients, symbols and sizes. • 48MB of disk space • Inverse distance (for 3D smoothing) • CD-ROM Drive • SVGA/256 color graphics adapter (800 x 600, High Color recommended) Learn more about SigmaPlot and other Systat products • To use HTML Help, Internet Explorer 4.01 or later required contact your nearest Systat office or visit us at www.systat.com * New Features added in SigmaPlot 10

North, Central & South America: Europe: UK and Ireland: Other Countries: Systat Software, Inc. Systat Software GmbH Systat Software UK Limited. Cranes Software International Ltd. 501 Canal Blvd, Suite E Schimmelbuschstr 25 23, Vista Centre, Shankar Narayan Building, 4th Floor, Block - I, Richmond, CA 94804-2028 D-40699 Erkrath 50, Salisbury Road, # 25 M.G. Road, USA Germany Hounslow, - TW4 6JQ, Bangalore - 560001, Phone: 800-797-7401 Phone: +49.2104.9540 London, UK. India. Fax: 800-797-7406 Phone (France only): 0800 90 37 55 Phone: +44-(0)208-538 0128 Phone: +91-80-4112 0000 Email: [email protected] Fax: +49.2104.95410 Fax: + 44-(0)208-538 0273 Fax: +91-80-4123 1274 Support: [email protected] E-mail: [email protected] Email: [email protected] Email: [email protected] Support: [email protected] Support: [email protected] Support: [email protected] © 2006 Systat Software, Inc. SigmaPlot, SigmaStat, SYSTAT, SigmaScan and SigmaScan Pro are registered trademarks of Systat Software, Inc. All other product or brand names are trademarks or registered trademarks of their respective holders.