SSH3 Antibody Cat. No.: 55-994
SSH3 Antibody
SSH3 Antibody immunohistochemistry analysis in formalin Flow cytometric analysis of Hela cells (right histogram) fixed and paraffin embedded human breast carcinoma compared to a negative control cell (left histogram).FITC- followed by peroxidase conjugation of the secondary conjugated goat-anti-rabbit secondary antibodies were antibody and DAB staining. used for the analysis.
Confocal immunofluorescent analysis of SSH3 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Specifications
October 1, 2021 1 https://www.prosci-inc.com/ssh3-antibody-55-994.html HOST SPECIES: Rabbit
SPECIES REACTIVITY: Human
This SSH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic IMMUNOGEN: peptide between 575-602 amino acids from the C-terminal region of human SSH3.
TESTED APPLICATIONS: Flow, IF, IHC-P, WB
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100 APPLICATIONS: For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
PREDICTED MOLECULAR 73 kDa WEIGHT:
Properties
This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification.
CLONALITY: Polyclonal
ISOTYPE: Rabbit Ig
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
CONCENTRATION: batch dependent
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
OFFICIAL SYMBOL: SSH3
Protein phosphatase Slingshot homolog 3, SSH-like protein 3, SSH-3L, hSSH-3L, SSH3, ALTERNATE NAMES: SSH3L
ACCESSION NO.: Q8TE77
PROTEIN GI NO.: 82582268
GENE ID: 54961
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
October 1, 2021 2 https://www.prosci-inc.com/ssh3-antibody-55-994.html Background and References
The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by BACKGROUND: kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).
REFERENCES: 1) Kousaka, K., et al. Genesis 46(5):246-255(2008)
2) Kligys, K., et al. J. Biol. Chem. 282(44):32520-32528(2007)
3) Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
4) Soosairajah, J., et al. EMBO J. 24(3):473-486(2005)
ANTIBODIES FOR RESEARCH USE ONLY.
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