SSH3 Antibody Cat. No.: 55-994 SSH3 Antibody SSH3 Antibody immunohistochemistry analysis in formalin Flow cytometric analysis of Hela cells (right histogram) fixed and paraffin embedded human breast carcinoma compared to a negative control cell (left histogram).FITC- followed by peroxidase conjugation of the secondary conjugated goat-anti-rabbit secondary antibodies were antibody and DAB staining. used for the analysis. Confocal immunofluorescent analysis of SSH3 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue). Specifications October 1, 2021 1 https://www.prosci-inc.com/ssh3-antibody-55-994.html HOST SPECIES: Rabbit SPECIES REACTIVITY: Human This SSH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic IMMUNOGEN: peptide between 575-602 amino acids from the C-terminal region of human SSH3. TESTED APPLICATIONS: Flow, IF, IHC-P, WB For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 APPLICATIONS: For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 PREDICTED MOLECULAR 73 kDa WEIGHT: Properties This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification. CLONALITY: Polyclonal ISOTYPE: Rabbit Ig CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide. CONCENTRATION: batch dependent Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: SSH3 Protein phosphatase Slingshot homolog 3, SSH-like protein 3, SSH-3L, hSSH-3L, SSH3, ALTERNATE NAMES: SSH3L ACCESSION NO.: Q8TE77 PROTEIN GI NO.: 82582268 GENE ID: 54961 USER NOTE: Optimal dilutions for each application to be determined by the researcher. October 1, 2021 2 https://www.prosci-inc.com/ssh3-antibody-55-994.html Background and References The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by BACKGROUND: kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]). REFERENCES: 1) Kousaka, K., et al. Genesis 46(5):246-255(2008) 2) Kligys, K., et al. J. Biol. Chem. 282(44):32520-32528(2007) 3) Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006) 4) Soosairajah, J., et al. EMBO J. 24(3):473-486(2005) ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. October 1, 2021 3 https://www.prosci-inc.com/ssh3-antibody-55-994.html.