Histology Staining - Reagents

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Histology Staining - Reagents Staining reagents, eosin, Thermo Scientific Shandon Gram’s staining solutions, ready to use Instant Eosin 04 LE A range of convenient ready prepared stains, which can be used immediately. • Stable and precipitate free – no Stain concentrates are 10 times concentrated to give a ready to use stain on dilution. filtering Catalogue No Description Volume, mL Price • Produces consistent results P/L500/08 Crystal violet 500 8.35 • pH-adjusted for maximum stain P/L500/15 Crystal violet 1,000 11.58 uptake P/L500/90 Crystal violet 2,000 15.30 • Produces brilliant staining P/L510/08 Gram’s iodine 500 9.38 P/L510/15 Gram’s iodine 1,000 15.43 • Compatible with all alum haematoxylin stains P/L510/90 Gram’s iodine 2,000 22.68 P/L520/08 Gram’s differentiator 500 9.91 • Economical powder, stores P/L520/15 Gram’s differentiator 1,000 16.72 indefinitely P/L520/90 Gram’s differentiator 2,000 25.85 • Available in either alcoholic or P/L530/08 Neutral red 500 9.64 aqueous formation P/L530/15 Neutral red 1,000 14.40 P/L530/90 Neutral red 2,000 18.13 Catalogue No Alt. No Description Pack qty Price P/L540/08 Safranin 500 11.70 SD6765040 6765040 Instant eosin, alcoholic, 6 88.95 P/L540/15 Safranin 1,000 15.56 makes 6L P/L540/90 Safranin 2,000 23.07 SD6765540 6765540 Instant eosin, aqueous, 6 88.95 P/L550/08 Dilute carbol fuchsin 500 8.10 makes 6L P/L550/15 Dilute carbol fuchsin 1,000 12.47 P/L550/90 Dilute carbol fuchsin 2,000 17.36 P/L560/08 Lugol’s iodine 500 10.94 Staining reagents, eosin, Thermo Scientific Shandon P/L560/15 Lugol’s iodine 1,000 18.25 Eosin Y P/L560/90 Lugol’s iodine 2,000 32.50 LE Staining reagent, haematoxylin, Thermo Scientific Shandon Instant Haematoxylin LE • No mucoid staining • Stains from blue-black to light blue depending on preparation • Protocol – progressive or regressive • Requires filtration depending on formula • Suitable for frozen sections, plastics, immuno histochemistry, special stains and cytology • May be used as a substitute for Harris or Gill stain Catalogue No Alt. No Description Pack qty Price • Stable and precipitate free – no filtering SD6765015 6765015 Instant haematoxylin, 6 109.66 makes 6 x 1L • Produces consistent results • pH-adjusted for maximum stain uptake • Produces brilliant staining • Compatible with all alum haematoxylin stains • Available in alcoholic or aqueous formulation • Ready to use • Fast acting cytoplasmic counter stain • Biological Stain Commission certified dye Catalogue No Alt. No Description Quantity, L Pack qty Price SD6766007 6766007 Eosin Y alcoholic 1 2 45.39 SD6766008 6766008 Eosin Y alcoholic 4 1 66.04 SD6766009 6766009 Eosin Y aqueous 1 2 45.09 SD6766010 6766010 Eosin Y aqueous 4 1 51.86 All products are for research use only, unless otherwise indicated. 1115.

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Eosin Staining
Science of H & E Andrew Lisowski, M.S., HTL (A.S.C.P.) 1 Hematoxylin and Eosin Staining “The desired end result of a tissue stained with hematoxylin and eosin is based upon what seems to be almost infinite factors. Pathologists have individual preferences for section thickness, intensities, and shades. The choice of which reagents to use must take into consideration: cost, method of staining, option of purchasing commercially-prepared or technician-prepared reagents, safety, administration policies, convenience, availability, quality, technical limitations, as well as personal preference.” Guidelines for Hematoxylin and Eosin Staining National Society for Histotechnology 2 Why Do We Stain? In order to deliver a medical diagnosis, tissues must be examined under a microscope. Once a tissue specimen has been processed by a histology lab and transferred onto a glass slide, it needs to be appropriately stained for microscopic evaluation. This is because unstained tissue lacks contrast: when viewed under the microscope, everything appears in uniform dull grey color. Unstained tissue H&E stained tissue 3 What Does "Staining" Do? . Contrasts different cells . Highlights particular features of interest . Illustrates different cell structures . Detects infiltrations or deposits in the tissue . Detect pathogens Superbly contrasted GI cells Placenta’s large blood H&E stain showing extensive vessels iron deposits There are different staining techniques to reveal different structures of the cell 4 What is H&E Staining? As its name suggests, H&E stain makes use of a combination of two dyes – hematoxylin and eosin. It is often termed as “routine staining” as it is the most common way of coloring otherwise transparent tissue specimen.
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Solarbio Science & Technology Co., Ltd Tel: 010-56371207 Solarbio Fax: 010-56371281/82
Beijing Solarbio Science & Technology Co., Ltd Tel: 010-56371207 Fax: 010-56371281/82 Solarbio Http://www.solarbio.cn Neutral Red CAS Number: 553-24-2 Storage Temperature: 2-8 °C Product Description : Appearance: Fine dark green-black powder Molecular Formula: C15H17ClN4 Molecular Weight: 288.78 Synonyms: toluylene red, basic red 5 Neutral Red is a weak cationic azine dye that is used extensively as a nuclear stain in a variety of biological stain applications. It is a pH indicator as well, changing color from red to yellow over the pH range 6.8-8.0. It is also incorporated into bacteriological growth media. This product is often used for supravital staining of fresh peripheral blood. It can also be used for staining Nissl granules of neuroglial cells. However, this stain is not as permanent as another dye, Cresyl Violet acetate, for this application. Buffered 0.5% Neutral Red solutions are used as a counterstain for Naphthol AS acetate esterase, peroxidase and iron stains. Solutions can also be used to stain plankton for viability. Using 1 part Neutral Red to 10,000 parts sea water, dead cells were stained red and live cells remained unchanged. In addition, aqueous solutions of Neutral Red (0.1% in saline, pH 6.5) can be used as a fluorescent stain for lipids. Lipids will fluoresce blue-geen or yellow, depending on their composition. It has been used also as a Twort's stain for parasites in combination with Light Green SF, as a general histological stain for embryonic tissue in combination with Janus green,and for demostrating hydrolysis of fats.
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STUDIES of MITOCHONDRIAL STAINING with PINACYANOLE, EMPLOYI}.TG YOSHIDA Ascittss SARCOMA CEI-L
247 STUDIES OF MITOCHONDRIAL STAINING WITH PINACYANOLE, EMPLOYI}.TG YOSHIDA ASCITtsS SARCOMA CEI-L KryosARU TexrrAwA, Kyoreno Aen, Krsuro Kero, Tnnuo Yosnloa AND Kyorcnr MesurANr Department of Internal Meclictne, I(omatsujima RerI Cross Hospital (Chief : Dr. Riyoharu Takikau)a) 7st Departntent of Internal Medicine, Nagoya Uniuersity School of Medicine (Director : Prof . Susumu Hibino) Because of potent activities of respiratory enzymes found in isolated mito- chondria, morphological changes in mitochondria have again attracted attention as indicative of cell's functional potentialities. Mitochondria in the cell can be visualized by employing, 1) Altmann's stain- ing method or Heidenhain's iron hematoxylin stain on fixed preparations, 2) the supravital staining method using Janus green, and recently 3) the supravital observation by means of the phase contrast microscope. Among these, the supravital method with Janus green is widely employed because of its simplicity and high specificity. But this Janus green method is not free from faults : namely difficulty in differentiating the types of cells and quick fading of the stained mitochondria. In 1936 Hetheringtonl) introduced a dyestuff named pinacyanole into the su- pravital staining method of mitochondria, and this method has been investigated by J. L. Schwind,2) showing that nuclei are stained supravitally and the types of cells are easily differentiated, stainability of neutral red vacuoleg is not dis- turbed and the colored mitochondria do not fade away for several hours. This pinacyanole (Consolidated Midland Corporation) and vital neutral red have been obtained lately, and we are discussing the usefulness of the former dyestuff in the study of mitochondria, comparing it with the above-mentioned various mitochondrial methods, and the nature of its staining mechanism.
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Basics of Hematology and Patho-Histology
Basics of Hematology and Patho-histology Practical Course in Molecular Pathology Winter Term 2015 Ernst Müllner MFPL (Max F Perutz Laboratories) Department of Medical Biochemistry Medical University of Vienna [email protected] www.mfpl.ac.at/mfpl-group/group/muellner.html (Müllner homepage / research) E. coli + macrophages medicalschool.tumblr.com/post/43914024728/sem-image-of-e-coli-bacteria-and-macrophages medicalschool.tumblr.com/post/18256087351/r ed-blood-cells-erythrocytes-trapped-by-fibrin Overview on main white blood cell (WBC) types – (Wikipedia) Mature white blood cell types I White Blood cells (WBCs) are frequently also referred to as peripheral blood mononuclear cells (PBMCs). Granulocytes in general are part of the innate immune system. Names derive from staining with hematoxylin and eosin. Whereas basophils stain dark blue and eosinophils are bright red, neutrophils stain neutral to pink. Basophil granulocytes Eosinophil granulocytes Neutrophil granulocytes Least common granulocyte type About 1-6% of WBCs; component Most abundant WBC type (40- (0.01- 0.3% of WBCs. Large of innate immune system to com- 75%) and essential part of the cytoplasmic granules obscure the bat parasites and certain infec- innate immune system. A patho- nucleus under the microscope. tions; also associated with allergy gen is likely to first encounter a When unstained, the nucleus is and asthma. Following activation, neutrophil. Normally contain a nu- visible and usually has 2 lobes. eosinophils effector functions in- cleus of 2-5 lobes. Neutrophils Basophils appear in inflammatory clude production and release (de- quickly congregate at a infection reactions, particularly those granulation) of cytotoxic substan- site, attracted by cytokines from causing allergies, mainly via the ces (granule proteins, reactive activated endothelium, mast cells, vasodilator histamine (antihistami- oxygen species …) and production or macrophages.
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||||||||||||III USO0575109A United States Patent (19) 11) Patent Number: 5,175,109 Sakata Et Al
||||||||||||III USO0575109A United States Patent (19) 11) Patent Number: 5,175,109 Sakata et al. (45) Date of Patent: " Dec. 29, 1992 54 REAGENT FOR CLASSIFYING 4,666.82 5/1987 Wiedemann et al. ................. 430/78 LEUKOCYTES BY FLOW CYTOMETRY 4,751,179 6/1988 Ledis et al. ........... ... 424/3 X 4,751,188 6/1988 Valet ............. 436/10 X 4.760.006 7/1988 Pawlowski ............................ 430/78 75) Inventors: Takashi Sakata; Tomoyuki Kuroda, both of Kakogawa, Japan 4.882,284 1 1/1989 Kirchanski et al. .. ... 436/63 73 Assignee: Toa Medical Electronics Co., Ltd., 4,933,293 6/1990 Kuroda et al. ........................ 436/63 Kobe, Japan FOREIGN PATENT DOCUMENTS *) Notice: The portion of the term of this patent O086951 8/1983 European Pat. Off. subsequent to Jun. 12, 2007 has been 1560729 2/1980 France . disclaimed. 55-18860 5/1980 Japan . (21) Appl. No.: 663,090 OTHER PUBLICATIONS Kamentsky, Blood Cells, 6, 121-140 (1980). (22 Filed: Feb. 28, 1991 Shapiro et al., J. Histochem. Cytochem., 24, 396-41 1, Related U.S. Application Data (1976). Shapiro et al., J. Histochem. Cytochem., 25, 976–989 63 Continuation of Ser. No. 91.663, Sep. 1, 1987, aban (1977). doned. Colour Index, vol. 4, published by The Society of Dyers (30) Foreign Application Priority Data and Colourists, pp. 4417-4459 (1971). Sep. 10, 1986 JP Japan ................................ 6-21376 Steinkamp, "Flow Cytometry," Rey. Sci. Instrum... pp. Nov. 27, 1986 JP Japan ................................ 6.-28.2697 1375-1400 (1974). W. Groner & D. Tycko, "Characterizing Blooc Cells 51) Int. Cl. .............................................. C09K11/06 52) U.S.
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STAINING TECHNIQUES Staining Is an Auxiliary Technique Used in Microscopy to Enhance Contrast in the Microscopic Image
STAINING TECHNIQUES Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains or dyes are used in biology and medicine to highlight structures in biological tissues for viewing with microscope. Cell staining is a technique that can be used to better visualize cells and cell components under a microscope. Using different stains, it is possible to stain preferentially certain cell components, such as a nucleus or a cell wall, or the entire cell. Most stains can be used on fixed, or non-living cells, while only some can be used on living cells; some stains can be used on either living or non-living cells. In biochemistry, staining involves adding a class specific (DNA, lipids, proteins or carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescence tagging can serve similar purposes Purposes of Staining The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope. Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a sample. Cells may also be enumerated by staining cells to determine biomass in an environment of interest. Stains may be used to define and examine bulk tissues (e.g. muscle fibers or connective tissues), cell populations (different blood cells) or organelles within individual cells. Biological staining is also used to mark cells in flow cytometry, flag proteins or nucleic acids on gel electrophoresis Staining is not limited to biological materials, it can also be used to study the morphology (form) of other materials e.g.
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Induced Osteogenesis in Plants Decellularized Scaffolds
www.nature.com/scientificreports OPEN Induced Osteogenesis in Plants Decellularized Scafolds Jennifer Lee1,4, Hyerin Jung2,3,4, Narae Park2,3, Sung-Hwan Park1 & Ji Hyeon Ju1,2,3* A three-dimensional (3D) culture system that closely replicates the in vivo microenvironment of calcifying osteoid is essential for in vitro cultivation of bone-like material. In this regard, the 3D cellulose constructs of plants may well serve as scafolds to promote growth and diferentiation of osteoblasts in culture. Our aim in this study was to generate bone-like tissue by seeding pluripotent stem cells (hiPSCs), stimulated to diferentiate as osteoblasts in culture, onto the decellularised scafolds of various plants. We then assessed expression levels of pertinent cellular markers and degrees of calcium- specifc staining to gauge technical success. Apple scafolding bearing regular pores of 300 μm seemed to provide the best construct. The bone-like tissue thus generated was implantable in a rat calvarial defect model where if helped form calcifed tissue. Depending on the regularity and sizing of scafold pores, this approach readily facilitates production of mineralized bone. It is well established that three-dimensional (3D), rather than two-dimensional (2D), culture ofers a microenvi- ronment closer to in vivo conditions, better enabling in vitro development of organoids. Similar with other organs, there is a growing clinical need for bone organoids, which may be particularly suitable substitutes for less available autologous bone grafs, helping to repair critical bony injuries or congenital defects. Unfortunately, current engi- neering techniques involving bone are largely restricted to 3D culture of osteoblasts. Hence, the term ‘bone-like tissue’ seems more apt than ‘bone organoid’.
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Product Name Here Routine Stains & Special Stains
® A Division of General Data Healthcare Histology Innovation for a NEW Generation Ready-To-Use Reagents, Dyes & Stains RoutineProduct Stains Name & SpecialHere Stains Single Source For Your Histology Reagents. Protecting Every Life Story In Your Lab. Each tissue specimen your lab processes has a life story behind it. Your mission is to ensure all your specimens receive optimal processing in order to deliver the best possible care. Our product line of histological reagents, dyes and ready-to-use stains enable your lab to deliver increased productivity, advanced specimen safety and provide highly accurate processing and staining results. ROutINE stAIns Eosin Y, 1.0% alcoholic solution, Non-Acidic Cytoplasmic counterstain useful in immuno-histochemistry or treatment of tissue sections with hematoxylin. Contains no acetic acid. Ready-to-use with any automated stainer. Cat. # Description E-1Y1P Eosin Y, 1%, 16 oz/ea; See MSDS for HAZ-MAT handling requirements under transportation on Air Shipments; Store at 25° C E-YP50GR Eosin Y Certified Powder, 50gm/ea. See MSDS for HAZ-MAT handling requirements under transportation on Air Shipments; Store at 25°C Gill’s #2, double strength for Histology & Cytology Used when a stronger or darker nuclear stain is required for cytology or immunohistochemistry (IHC) counterstaining. Gill’s #2 formulation is a double strength mixture, stains darker and more quickly than Gill’s #1. General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining. Cat. # Description H-G21L Gills 2 Hematoxylin, 1L/ea. See MSDS for HAZ-MAT handling requirements under transportation on Air Shipments; Store at room temp.
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Developing Fluorogenic Reagents for Detecting and Enhancing Bloody Fingerprints
The author(s) shown below used Federal funds provided by the U.S. Department of Justice and prepared the following final report: Document Title: Developing Fluorogenic Reagents for Detecting and Enhancing Bloody Fingerprints Author: Robert M. Strongin, Dr.Martha Sibrian-Vazquez Document No.: 227841 Date Received: August 2009 Award Number: 2007-DN-BX-K171 This report has not been published by the U.S. Department of Justice. To provide better customer service, NCJRS has made this Federally- funded grant final report available electronically in addition to traditional paper copies. Opinions or points of view expressed are those of the author(s) and do not necessarily reflect the official position or policies of the U.S. Department of Justice. Developing Fluorogenic Reagents for Detecting and Enhancing Bloody Fingerprints Award 2007-DN-BX-K171 Authors Prof. Robert M. Strongin Dr.Martha Sibrian-Vazquez 1 This document is a research report submitted to the U.S. Department of Justice. This report has not been published by the Department. Opinions or points of view expressed are those of the author(s) and do not necessarily reflect the official position or policies of the U.S. Department of Justice. Abstract Fingerprints are the most common and useful physical evidence for the apprehension and conviction of crime perpetrators. Fluorogenic reagents for detecting and enhancing fingerprints in blood, however, have several associated challenges. For instance, they are generally unsuitable for dark and multi-colored substrates. Luminol and fluorescin and other chemilumigens and fluorigens can be used with dark and often multi-colored substrates, but are not compatible with fixatives and their oxidation products are not insoluble.
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Morphological Assessment of Epididymal Sperm in Wistar Rats Using Different Histological Stains
Short Communication Acta Vet Eurasia 2020; 46: 132-136 Morphological Assessment of Epididymal Sperm in Wistar Rats Using Different Histological Stains Nathan Isaac DIBAL , Sani Hyedima GARBA , Tamunotonye Watson JACKS Department of Human Anatomy, University of Maiduguri, Maiduguri, Nigeria Cite this article as: Dibal, N.I., Garba, S.H., Jacks, T.W., 2020. Morphological Assessment of Epididymal Sperm in Wistar Rats Using Different Histological Stains. Acta Vet Eurasia 2020; 46: 132-136. ORCID IDs of the authors: N.I.D. 0000-0002-1805-7553; S.H.G. 0000-0001-6165-0064; T.W.J. 0000-0002-1096-2997 Abstract Spermatozoa are unique and highly specialized cells operat- blue, Alcian blue, Harris hematoxylin, and eosin stains. The re- ing at the microscopic level in a complex environment and sult showed that the head and the proximal and distal parts have various sizes, shapes, and forms. The sizes, shapes, and of the tail were stained excellently with crystal violet and H&E forms of the spermatozoa are dependent on the species but stains. Giemsa and Harris hematoxylin were also good but may be different even within the same species. Five Wistar rats could not stain the distal part of the tail. In conclusion, crystal were euthanized using ketamine injection. The epididymis of violet is the best stain for sperm morphology in Wistar rats fol- each rat was dissected, teased and diluted in normal saline. lowed by H&E stain. Several smears were prepared and stained with Giemsa, Leish- Keywords: Crystal violet, eosin, hematoxylin, morphology, man, hematoxylin and eosin (H&E), crystal violet, methylene sperm Introduction copy (Sousa et al., 2013; Villaverde et al., 2008).
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GHS,Hematoxylin Stains Procedure
HEMATOXYLIN STAINS 4. If eosin staining is excessive, nuclear staining may be masked. Proper eosin staining will (Procedure No. GHS) demonstrate a 3-tone effect. To increase dif feren ti a tion of eosin, extend time in alcohols or use a first alcohol with a higher water content. The times in the alcohols may be _______________________________________________ adjusted to obtain the proper degree of eosin staining. 5. Positive control slides should be included in each run. INTENDED USE 6. The data obtained from this procedure serves only as an aid to diagnosis and should be _______________________________________________ reviewed in conjunction with other clinical diagnostic tests or information. Gill Hematoxylin solutions are nuclear stains intended for use in Histology and Cytology. Hematoxylin Solutions, Gill Nos. 1, 2 and 3 are for “In Vitro Diagnostic Use”. PROCEDURE 1: Hematoxylin, a common nuclear stain, is isolated from an extract of logwood (Staining Exfoliative Cytology Preparations Using Hematoxylin Solution, Gill No. 1 or Gill No. 2) (Haematoxylon campechianum).1 The first successful bi o log ic application of hematoxylin 1. Fix cytologic smears in 95% ethanol..................................................................15 minutes was described by Bohmer1 in 1865. Since then numerous formulations have appeared. Of 2. Rinse in gently running tap water.......................................................................30 seconds these, Harris’, Gill’s, Mayer’s and Weigert’s have retained popularity. Before hematoxylin can 3. Stain in Hematoxylin Solution, Gill No. 1 or Gill No. 2.....................................1.5-3 minutes be used as a nuclear stain, it must be oxidized to hematein and combined with a metallic ion 4. Rinse in tap water. (mordant). Most successful mordants have been salts of aluminum or iron.
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Article Text Additional Text Cinr Schulznr Casnr Item Number
The whole world of dyes and dye solutions Chroma-products – subdivided in dyes, dye solutions, indicators and auxiliaries article text additional text item number CINr SchulzNr CASNr hazardous Acid blue 119 1B-555 42765 1324-76-1 Carbolic Gentian Violet 2E-028K Y China Blue 1B-507 42755 816 discoloration solution acetone/Ethanol 1:1 E333 Y nuclear fast red solution (0,1%) aqueous 2C-337 6409-77-4 rhodamine b, ethanolic (1%) 2C-339 64-17-5 Y Safranin solution 2C-333K Y shipping cost chroma solvent green 3 1B-553 128-80-3 staining reagent eppendahl II 1A-652 Water Blue TR, Unna 1B-517 42755 816 Dienstag, 28. September 2021 SEITE 1 VON 21 The whole world of dyes and dye solutions Chroma-products – subdivided in dyes, dye solutions, indicators and auxiliaries article text additional text item number CINr SchulzNr CASNr hazardous dyes Acid Alizarine Blue B 1A-252 16680 1058-92-0 Acid Black 12 B 1A-598 20470 299 1064-48-8 acid brilliant flavine 7g 1F-562 61968-07-8 Acid Fuchsine-Orange 1F-347 Acid Green G 1B-215 42095 765 5141-20-8 Acid Rhodamine 1A-004 45100 863 3520-42-1 Acridine Orange 3 R zinc chloride double salt 1B-307 46005 10127-02-3 Acridine Yellow 1B-331 46025 135-49-9 Acriflavine 5A-406 46000 906 Y Alcian Green 2 GX 1F-555 Alcian Green 3 BX 1F-551 Alcian Yellow GXS 1F-597 12840 61968-76-1 Alizarine Blue B 1A-246 16680 Alizarine Brilliant Violet R 1B-077 60730 1196 4430-18-6 Alizarine Carmine 1F-581 58005 1145 130-22-3 Alizarine Pure 1A-020 58000 1141 72-48-0 Alizarine Purple RS 1B-079 60730 1196 4430-18-6 Alizarine Red S 1F-583 58005
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