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A Study of Rawitz's 'Inversion Staining' by ALEKSANDRA PRZEL^CKA
231 A Study of Rawitz's 'Inversion Staining' By ALEKSANDRA PRZEL^CKA {From the Cytological Laboratory, Department of Zoology, University Museum, Oxford, and the Nencki Institute, 3 Pasteur St., Warsaw 22; present address, Nencki Institute) SUMMAHY The Rawitz method involves mordanting with tannic acid and potassium antimony tartrate, and staining with basic fuchsine. The mordanting causes basic fuchsine to act as though it were an acid dye ('inversion staining'). A modification of the method is described in the present paper. This modification makes it possible to obtain the same results in a shorter time. The chief substances stained by Rawitz's method are phospholipids, certain pro- teins, and certain polysaccharides. Although the method cannot be regarded as a cytochemical test in the strict sense, yet it gives useful indications of chemical composition and in addition is valuable to the morphological cytologist as a technique for showing certain cytoplasmic inclusions (mitotic spindle, acrosome, mitochondria, 'Golgi apparatus' of certain cells). INTRODUCTION T is well known that the so-called 'Golgi apparatus' is extremely difficult to I reveal by any staining method. Baker, in the course of his investigation on this organelle in the epididymis of the mouse, found that it can be stained by basic fuchsin after a special mordanting process (1957). The method was taken from Rawitz (1895), who found that basic fuchsin, if mordanted with tannic acid and potassium antimony tartrate, loses the character of a dye for chro- matin and colours the cytoplasm instead. Rawitz called this effect 'inversion staining'. Since this technique, when applied to various kinds of cytological material, gave good selectivity in visualizing certain delicate cell structures, it seemed interesting to investigate the nature of the chemical compounds which are responsible for positive Rawitz staining. -
Lysochrome Dyes Sudan Dyes, Oil Red Fat Soluble Dyes Used for Biochemical Staining of Triglycerides, Fatty Acids, and Lipoproteins Product Description
FT-N13862 Lysochrome dyes Sudan dyes, Oil red Fat soluble dyes used for biochemical staining of triglycerides, fatty acids, and lipoproteins Product Description Name : Sudan IV Other names: Sudan R, C.I. Solvent Red 24, C.I. 26105, Lipid Crimson, Oil Red, Oil Red BB, Fat Red B, Oil Red IV, Scarlet Red, Scarlet Red N.F, Scarlet Red Scharlach, Scarlet R Catalog Number : N13862, 100g Structure : CAS: [85-83-6] Molecular Weight : MW: 380.45 λabs = 513-529 nm (red); Sol(EtOH): 0.09%abs =513-529nm(red);Sol(EtOH):0.09% S:22/23/24/25 Name : Sudan III Other names: Rouge Sudan ; rouge Ceresin ; CI 26100; CI Solvent Red 23 Catalog Number : 08002A, 25g Structure : CAS:[85-86-9] Molecular Weight : MW: 352.40 λabs = 513-529 nm (red); Sol(EtOH): 0.09%abs =503-510nm(red);Sol(EtOH):0.15% S:24/25 Name : Sudan Black B Other names: Sudan Black; Fat Black HB; Solvent Black 3; C.I. 26150 Catalog Number : 279042, 50g AR7910, 100tests stain for lipids granules Structure : CAS: [4197-25-5] S:22/23/24/25 Molecular Weight : MW: 456.54 λabs = 513-529 nm (red); Sol(EtOH): 0.09%abs=596-605nm(blue-black) Name : Oil Red O Other names: Solvent Red 27, Sudan Red 5B, C.I. 26125 Catalog Number : N13002, 100g Structure : CAS: [1320-06-5 ] Molecular Weight : MW: 408.51 λabs = 513-529 nm (red); Sol(EtOH): 0.09%abs =518(359)nm(red);Sol(EtOH): moderate; Sol(water): Insoluble S:22/23/24/25 Storage: Room temperature (Z) P.1 FT-N13862 Technical information & Directions for use A lysochrome is a fat soluble dye that have high affinity to fats, therefore are used for biochemical staining of triglycerides, fatty acids, and lipoproteins. -
Revisions Inserts Rev from Rev to JOB
BALTSO0191 Version 11.0 Template 4 Revisions Inserts Rev from Rev to JOB # 06 07 52-17 Notes: 1. BD Catalog Number: 212525, 212526, 212527, 212528, 212531, 212532, 212539, 212542, 212543, 212544, 212545 2. Blank (Sheet) Size: Length: 25.5” Width: 22” 3. Number of Pages: 28 Number of Sheets: 1 4. Page Size: Length: 8.5” Width: 5.5” Final Folded Size: 4.25” x 5.5” 5. Ink Colors: No. of Colors: 2 PMS#: 032 Red; Standard Black 6. Printed two sides: Yes X No 7. Style (see illustrations below): # 5 W W W W W W W 8. Vendor Printed X Online/In House Printed Web 9. See specication control no. N/A for material information. 10. Graphics are approved by Becton, Dickinson and Company. Supplier has the responsibility for using the most current approved revision level. Label Design COMPANY CONFIDENTIAL. THIS DOCUMENT IS THE PROPERTY OF BECTON, DICKINSON AND Becton, Dickinson and Company Proofer COMPANY AND IS NOT TO BE USED OUTSIDE THE COMPANY WITHOUT WRITTEN PERMISSION. 7 Loveton Circle Sparks, MD 21152 USA Checked By Category and Description Sheet: 1 of 29 Part Number: Package Insert, 8820191JAA Gram Stain Kits and Reagents Scale: N/A A B Gram Stain Kits and Reagents English: pages 1 – 5 Italiano: pagine 14 – 18 8820191JAA(07) Français : pages 5 – 9 Español: páginas 19 – 23 2017-09 Deutsch: Seiten 10 – 14 Contact your local BD representative for instructions. / Свържете се с местния представител на BD за инструкзии. / Pokyny vám poskytne místní zástupce společnosti BD. / Kontakt den lokale BD repræsentant for at få instruktioner. -
Digitally Reinforced Polarization of Hematoxylin-Eosin in the Diagnosis
Özgün Araştırma/Original Article doi: 10.5146/tjpath.2012.01126 Digitally Reinforced Polarization of Hematoxylin-Eosin in the Diagnosis of Renal Amyloidosis Renal Amiloidoz Tanısında Dijital Güçlendirilmiş Hematoksilen Eozin Polarizasyonu Sait ŞEN, Banu SARSIK KumbaraCI Department of Medical Pathology, Ege University, Faculty of Medicine, İZMİR, TURKEY The summary of this study was presented at 24th Congress of Pathology held in Prague on 8-12 September 2012 ABSTRACT ÖZ Objective: Systemic amyloidosis is a rare disorder, characterized by Amaç: Sistemik amiloidozlar, hematoksilen-eozin boyamada amorf extracellular accumulation of Congo red positive fibrillar amyloid eozinofilik görülen, Kongo kırmızısı ile boyanan fibriller amiloid protein deposits that have an amorphous, eosinophilic appearance proteinlerin ekstrasellüler birikimiyle karakterize nadir hastalıklardır. on hematoxylin-eosin stained preparations. The kidney is the Böbrekler sistemik amiloidozlardan en sık etkilenen organdır. Kongo most commonly affected organ by systemic amyloidosis. Congo kırmızısı, zayıf birefrenjant boyanmamış amiloidin birefranjansını red staining increases the positive birefringence of the weakly artırır. Bu çalışmada, böbrek biopsilerinin rutin hematoksilen eozin birefringent unstained amyloid. In this study, we investigated the kesitlerinde dijital güçlendirilmiş birefrenjansın potansiyel tanısal potential diagnostic power of digitally reinforced birefringence of gücünü araştırdık. routine hematoxylin-eosin stained slides from renal biopsies. Gereç ve Yöntem: Hematoksilen-eozin boyalı 130 preparat Material and Method: We reviewed 130 hematoxylin-eosin stained polarizasyon için değerlendirildi. Altmış beş yeni amiloidoz olgusuna slides for polarization. Sixty-five new amyloidosis cases were böbrek biyopsisi ile tanı konuldu. Tüm böbrek biopsileri ışık ve diagnosed by renal biopsy. All renal biopsies were evaluated by light immünflöresan mikroskop ile değerlendirildi. Preparatlar kör olarak, microscopy and immunofluorescence. -
Eosin Staining
Science of H & E Andrew Lisowski, M.S., HTL (A.S.C.P.) 1 Hematoxylin and Eosin Staining “The desired end result of a tissue stained with hematoxylin and eosin is based upon what seems to be almost infinite factors. Pathologists have individual preferences for section thickness, intensities, and shades. The choice of which reagents to use must take into consideration: cost, method of staining, option of purchasing commercially-prepared or technician-prepared reagents, safety, administration policies, convenience, availability, quality, technical limitations, as well as personal preference.” Guidelines for Hematoxylin and Eosin Staining National Society for Histotechnology 2 Why Do We Stain? In order to deliver a medical diagnosis, tissues must be examined under a microscope. Once a tissue specimen has been processed by a histology lab and transferred onto a glass slide, it needs to be appropriately stained for microscopic evaluation. This is because unstained tissue lacks contrast: when viewed under the microscope, everything appears in uniform dull grey color. Unstained tissue H&E stained tissue 3 What Does "Staining" Do? . Contrasts different cells . Highlights particular features of interest . Illustrates different cell structures . Detects infiltrations or deposits in the tissue . Detect pathogens Superbly contrasted GI cells Placenta’s large blood H&E stain showing extensive vessels iron deposits There are different staining techniques to reveal different structures of the cell 4 What is H&E Staining? As its name suggests, H&E stain makes use of a combination of two dyes – hematoxylin and eosin. It is often termed as “routine staining” as it is the most common way of coloring otherwise transparent tissue specimen. -
Factors Affecting the Adsorption of Some Ionic Dyes on the Surface of Modify Cao from Eggshell
Asian Journal of Applied Sciences (ISSN: 2321 – 0893) Volume 07 – Issue 01, February 2019 Factors Affecting the Adsorption of Some Ionic Dyes on the Surface of Modify CaO from Eggshell Ibtighaa K. Radhi, Mouayed A. Hussein, Zaki N. Kadhim* Department of Chemistry, College of Science, University of Basrah Basrah, Iraq *Corresponding author’s emails: zekinasser99 [AT] yahoo.com ________________________________________________________________________________________________ ABSTRACT--- In this paper, calcium oxide (CaO) was produced by the thermal treatment of eggshell. The doping process with silver iodide (AgI), oxygen (O), sulfur(S) and nitrogen (N) was achieved by adsorbents. The adsorption of Acid fuchsine (AF), Indigo Carmine (IC), Nigrosine (NG) and Alizarine Red S (AR) on the surface of these particles was studied. The different conditions affecting the adsorption process, such as the time of equilibrium, the primary concentration of the studied dyes, the amount of the adsorbent, the acidic function, the speed of the pruning motion and the temperature were studied. The pH stability time (5-10 minutes), IC and NG (30 minutes) and AR were (90 minutes). The effect of temperature was also studied within the range (25-45 ° C). The results showed that the adsorption capacity increased by increasing the temperature, ie the reaction is endothermic. The study showed the effect of the acidic function on the percentage of pigmentation. The percentage was increased by increasing the acidic function in the basal circles on the surfaces except for the AR dye. It decreased the percentage by increasing the acidic function. The effect of the weight of the adsorbent was studied on the percentage of adsorption. -
Solarbio Science & Technology Co., Ltd Tel: 010-56371207 Solarbio Fax: 010-56371281/82
Beijing Solarbio Science & Technology Co., Ltd Tel: 010-56371207 Fax: 010-56371281/82 Solarbio Http://www.solarbio.cn Neutral Red CAS Number: 553-24-2 Storage Temperature: 2-8 °C Product Description : Appearance: Fine dark green-black powder Molecular Formula: C15H17ClN4 Molecular Weight: 288.78 Synonyms: toluylene red, basic red 5 Neutral Red is a weak cationic azine dye that is used extensively as a nuclear stain in a variety of biological stain applications. It is a pH indicator as well, changing color from red to yellow over the pH range 6.8-8.0. It is also incorporated into bacteriological growth media. This product is often used for supravital staining of fresh peripheral blood. It can also be used for staining Nissl granules of neuroglial cells. However, this stain is not as permanent as another dye, Cresyl Violet acetate, for this application. Buffered 0.5% Neutral Red solutions are used as a counterstain for Naphthol AS acetate esterase, peroxidase and iron stains. Solutions can also be used to stain plankton for viability. Using 1 part Neutral Red to 10,000 parts sea water, dead cells were stained red and live cells remained unchanged. In addition, aqueous solutions of Neutral Red (0.1% in saline, pH 6.5) can be used as a fluorescent stain for lipids. Lipids will fluoresce blue-geen or yellow, depending on their composition. It has been used also as a Twort's stain for parasites in combination with Light Green SF, as a general histological stain for embryonic tissue in combination with Janus green,and for demostrating hydrolysis of fats. -
STUDIES of MITOCHONDRIAL STAINING with PINACYANOLE, EMPLOYI}.TG YOSHIDA Ascittss SARCOMA CEI-L
247 STUDIES OF MITOCHONDRIAL STAINING WITH PINACYANOLE, EMPLOYI}.TG YOSHIDA ASCITtsS SARCOMA CEI-L KryosARU TexrrAwA, Kyoreno Aen, Krsuro Kero, Tnnuo Yosnloa AND Kyorcnr MesurANr Department of Internal Meclictne, I(omatsujima RerI Cross Hospital (Chief : Dr. Riyoharu Takikau)a) 7st Departntent of Internal Medicine, Nagoya Uniuersity School of Medicine (Director : Prof . Susumu Hibino) Because of potent activities of respiratory enzymes found in isolated mito- chondria, morphological changes in mitochondria have again attracted attention as indicative of cell's functional potentialities. Mitochondria in the cell can be visualized by employing, 1) Altmann's stain- ing method or Heidenhain's iron hematoxylin stain on fixed preparations, 2) the supravital staining method using Janus green, and recently 3) the supravital observation by means of the phase contrast microscope. Among these, the supravital method with Janus green is widely employed because of its simplicity and high specificity. But this Janus green method is not free from faults : namely difficulty in differentiating the types of cells and quick fading of the stained mitochondria. In 1936 Hetheringtonl) introduced a dyestuff named pinacyanole into the su- pravital staining method of mitochondria, and this method has been investigated by J. L. Schwind,2) showing that nuclei are stained supravitally and the types of cells are easily differentiated, stainability of neutral red vacuoleg is not dis- turbed and the colored mitochondria do not fade away for several hours. This pinacyanole (Consolidated Midland Corporation) and vital neutral red have been obtained lately, and we are discussing the usefulness of the former dyestuff in the study of mitochondria, comparing it with the above-mentioned various mitochondrial methods, and the nature of its staining mechanism. -
Basics of Hematology and Patho-Histology
Basics of Hematology and Patho-histology Practical Course in Molecular Pathology Winter Term 2015 Ernst Müllner MFPL (Max F Perutz Laboratories) Department of Medical Biochemistry Medical University of Vienna [email protected] www.mfpl.ac.at/mfpl-group/group/muellner.html (Müllner homepage / research) E. coli + macrophages medicalschool.tumblr.com/post/43914024728/sem-image-of-e-coli-bacteria-and-macrophages medicalschool.tumblr.com/post/18256087351/r ed-blood-cells-erythrocytes-trapped-by-fibrin Overview on main white blood cell (WBC) types – (Wikipedia) Mature white blood cell types I White Blood cells (WBCs) are frequently also referred to as peripheral blood mononuclear cells (PBMCs). Granulocytes in general are part of the innate immune system. Names derive from staining with hematoxylin and eosin. Whereas basophils stain dark blue and eosinophils are bright red, neutrophils stain neutral to pink. Basophil granulocytes Eosinophil granulocytes Neutrophil granulocytes Least common granulocyte type About 1-6% of WBCs; component Most abundant WBC type (40- (0.01- 0.3% of WBCs. Large of innate immune system to com- 75%) and essential part of the cytoplasmic granules obscure the bat parasites and certain infec- innate immune system. A patho- nucleus under the microscope. tions; also associated with allergy gen is likely to first encounter a When unstained, the nucleus is and asthma. Following activation, neutrophil. Normally contain a nu- visible and usually has 2 lobes. eosinophils effector functions in- cleus of 2-5 lobes. Neutrophils Basophils appear in inflammatory clude production and release (de- quickly congregate at a infection reactions, particularly those granulation) of cytotoxic substan- site, attracted by cytokines from causing allergies, mainly via the ces (granule proteins, reactive activated endothelium, mast cells, vasodilator histamine (antihistami- oxygen species …) and production or macrophages. -
LAB 3: Morphological Characteristics of Bacteria Protocols for Endospore Stain, Capsule Stain, Motility Stab and Wet Mount
LAB 3: Morphological Characteristics of Bacteria Protocols for Endospore Stain, Capsule Stain, Motility Stab and Wet Mount. INTRODUCTION Bacteria are characterized by the presence or absence of a number of different structures. Endospores, capsules and flagella are three such examples. Each of these structures is visible with light microscopy if the correct staining procedure is employed. ENDOSPORES are survival structures. In poor growth conditions some genera may sporulate. Rather than dying, endospores survive in a dormant state. Endospores are unique to Bacteria and are formed by a limited number of bacterial genera. The soil bacteria within the genera Bacillus and Clostridium are the most familiar. The stepwise process of sporulation is triggered by poor growth conditions ( see the discussion of the process of sporulation in your text). The transition from vegetative cell to endopsore requires an environmental signal and then a series of steps. The The endospore forms within the vegetative cell. A wall forms around a copy of the bacterial chromosome, capturing some ribosomes, proteints and DNA. The endospore forming within the cell can be visualized using the light microscope. As the sporulation process continues, layers form within the spore making it very dense. Exterior to the spore, the vegetative cell dies. At the completion of sporulation, oval spores are visible using light microscopy. Endospores cannot replicate. However they allow survival in lean times. In fact they are resistant to extreme environmental conditions such as high temperatures, dryness, toxic chemicals, and UV radiation. The dormant structure allows cell survival until conditions favorable to cell growth returns. Favorable growth conditions signal the process of endospore germination. -
||||||||||||III USO0575109A United States Patent (19) 11) Patent Number: 5,175,109 Sakata Et Al
||||||||||||III USO0575109A United States Patent (19) 11) Patent Number: 5,175,109 Sakata et al. (45) Date of Patent: " Dec. 29, 1992 54 REAGENT FOR CLASSIFYING 4,666.82 5/1987 Wiedemann et al. ................. 430/78 LEUKOCYTES BY FLOW CYTOMETRY 4,751,179 6/1988 Ledis et al. ........... ... 424/3 X 4,751,188 6/1988 Valet ............. 436/10 X 4.760.006 7/1988 Pawlowski ............................ 430/78 75) Inventors: Takashi Sakata; Tomoyuki Kuroda, both of Kakogawa, Japan 4.882,284 1 1/1989 Kirchanski et al. .. ... 436/63 73 Assignee: Toa Medical Electronics Co., Ltd., 4,933,293 6/1990 Kuroda et al. ........................ 436/63 Kobe, Japan FOREIGN PATENT DOCUMENTS *) Notice: The portion of the term of this patent O086951 8/1983 European Pat. Off. subsequent to Jun. 12, 2007 has been 1560729 2/1980 France . disclaimed. 55-18860 5/1980 Japan . (21) Appl. No.: 663,090 OTHER PUBLICATIONS Kamentsky, Blood Cells, 6, 121-140 (1980). (22 Filed: Feb. 28, 1991 Shapiro et al., J. Histochem. Cytochem., 24, 396-41 1, Related U.S. Application Data (1976). Shapiro et al., J. Histochem. Cytochem., 25, 976–989 63 Continuation of Ser. No. 91.663, Sep. 1, 1987, aban (1977). doned. Colour Index, vol. 4, published by The Society of Dyers (30) Foreign Application Priority Data and Colourists, pp. 4417-4459 (1971). Sep. 10, 1986 JP Japan ................................ 6-21376 Steinkamp, "Flow Cytometry," Rey. Sci. Instrum... pp. Nov. 27, 1986 JP Japan ................................ 6.-28.2697 1375-1400 (1974). W. Groner & D. Tycko, "Characterizing Blooc Cells 51) Int. Cl. .............................................. C09K11/06 52) U.S. -
Colloid Milium: a Histochemical Study* James H
CORE Metadata, citation and similar papers at core.ac.uk Provided by Elsevier - Publisher Connector THE JOURNAL OF INVESTIOATIVE DERMATOLOOY vol. 49, No. 5 Copyright 1567 by The Williams & Wilkins Co. Printed in U.S.A. COLLOID MILIUM: A HISTOCHEMICAL STUDY* JAMES H. GRAHAM, M.D. AND ANTONIO S. MARQUES, M.D. Wagner (1), in 1866, first reported colloidreaction, with and without diastase digestion; milium in a 54 year old woman who showedcolloidal iron reaction, with and without bovine testicular hyaluronidase digestion for 1 hour at lesions on the forehead, cheeks and nose. In37 C; Movat's pentachrome I stain (2); alcian patients with colloid milium, the involvedblue pH 2.5 and 0.4 (3, 4); aldehyde-fuchsin pH skin is usually hyperpigmented, thickened,1.7 and 0.4 (4), with and without elastase digestion furrowed, nnd covered with multiple 0.5—5(5); Snook's reticulum stain; phosphotungstic acid hematoxylin stain (PTAH); Prussian blue re- mm dome-shaped, discrete papules. The shiny,action for iron; Fontana-Masson stain for ar- pink or orange to yellowish white translucentgentaffin granules; thiofiavine T fluorescent stain lesions have been likened to vesieles, but are(6, 7); Congo red; alkaline Congo red method firm and only after considerable pressure can(8); crystal violet amyloid stain; methyl violet a clear to yellow mueoid substance be ex-stain for amyloid (9, 5); toluidine blue (4); and Giemsa stain. The crystal violet and methyl vio- pressed from the papules. The lesions involvelet stained sections were mounted in Highman's sun exposed sites including the dorsum of theApathy gum syrup (5) which tends to prevent hands, web between the thumb and indexbleeding and gives a more permanent preparation.