Field-Based Evaluation of Two Herbaceous Plant Community Sampling Methods for Long-Term Monitoring in Northern Great Plains National Parks
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In cooperation with the National Park Service Field-Based Evaluation of Two Herbaceous Plant Community Sampling Methods for Long-Term Monitoring in Northern Great Plains National Parks By Amy J. Symstad, Cody L. Wienk, and Andy Thorstenson Open-File Report 2006-1282 U.S. Department of the Interior U.S. Geological Survey 1 U.S. Department of the Interior Gale A. Norton, Secretary U.S. Geological Survey P. Patrick Leahy, Acting Director U.S. Geological Survey, Reston, Virginia 2006 For product and ordering information: World Wide Web: http://www.usgs.gov/pubprod Telephone: 1-888-ASK-USGS For more information on the USGS—the Federal source for science about the Earth, its natural and living resources, natural hazards, and the environment: World Wide Web: http://www.usgs.gov Telephone: 1-888-ASK-USGS Suggested citation: Symstad, A.J., Wienk, C.L., and Thorstenson, Andy, 2006, Field-based evaluation of two herbaceous plant community sampling methods for long-term monitoring in northern Great Plains national parks: Helena, MT, U.S. Geological Survey Open-File Report 2006-1282, 38 pages + 3 appendices. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. Although this report is in the public domain, permission must be secured from the individual copyright owners to reproduce any copyrighted material contained within this report. 2 Contents Contents ...............................................................................................................................................................................3 Introduction .........................................................................................................................................................................5 Vegetation in Northern Great Plains Parks................................................................................................................5 Potential Methods for Sampling Plant Community Composition ...........................................................................6 Study Objectives .............................................................................................................................................................8 Methods ...............................................................................................................................................................................8 Study Areas .....................................................................................................................................................................8 Sample Sites....................................................................................................................................................................9 Sampling.........................................................................................................................................................................11 Data Analysis.................................................................................................................................................................12 Results ................................................................................................................................................................................14 Between-Method Comparison of Values Obtained................................................................................................16 Repeatability..................................................................................................................................................................19 Number of Subsamples ...............................................................................................................................................20 Efficiency........................................................................................................................................................................27 Discussion..........................................................................................................................................................................29 Comparison of Values Obtained by Each Method..................................................................................................30 Repeatability among Observers.................................................................................................................................30 Precision and Number of Subsamples .....................................................................................................................32 Efficiency........................................................................................................................................................................33 Impacts of Sampling on Vegetation ..........................................................................................................................34 Conclusions and Recommendations.............................................................................................................................34 References Cited ..............................................................................................................................................................37 Figures Figure 1. Locations of NPS units in the Northern Great Plains I&M Network. The parks used in this study are underlined in red.................................................................................................................................. 9 Figure 2. Rank (x-axis)-abundance (y-axis) curves for the 46 plots sampled, by vegetation type. Each line-symbol combination within a graph represents a single plot. ............................................................ 15 Figure 3. Relationship between community-level response variable values obtained by the two sampling methods. Dashed lines are y=x....................................................................................................... 17 Figure 4. Least squares means and standard errors for log-transformed coefficients of variation among subsamples within plots for six response variables, by vegetation type. Different letters above symbols within a graph indicate significant (P < 0.05) differences between vegetation types. Vegetation type abbreviations follow those in Table 1.................................................................................21 Figure 5. Species-sample number curves for plots with (A) the lowest and (B) the highest species richness from each vegetation type. Solid lines are for the ocular method, dashed lines for the point-intercept method. (C) Difference between ocular and point-intercept methods in the number 3 of species added with an additional subsample. Open symbols are for plots with high species richness, closed symbols for plots with low species richness...................................................................25 Figure 6. Relationship between time to complete each sampling method (ocular = black circles and solid line; point-intercept = red triangles and dashed line) and (A) plot species richness or (B) total plant cover as measured by the point-intercept method............................................................................. 27 Figure 7. Number of times a method (point-intercept = black bars; ocular = red bars) did not detect a species that the other method did, for all 46 plots. ....................................................................................... 28 Figure 8. Photos of plots showing variability in terrain and obstructions to transect tapes. (A) TPCM03, (B) WPCM09, (C) WPCM12. .............................................................................................................. 32 Figure 9. Suggested modification of transect layout for point-intercept sampling. Red “X” indicates permanent marker. Black dots are approximate locations of points, spaced at 1-m intervals. Blue boxes are 1-m2 species-occurrence subplots................................................................................................ 35 Tables Table 1. Number of sample locations in each vegetation type for each park. ........................................ 10 Table 2. Mean and range (in parentheses) of variables descriptive of vegetation in each vegetation type sampled in this study.................................................................................................................................. 14 Table 3. Paired t-test and correlation results for point-intercept vs. ocular sampling methods for six community response variables......................................................................................................................... 16 Table 4.Comparison of repeatability for two methods of vegetation sampling based on correlation coefficients and paired t-tests.......................................................................................................................... 18 Table 5. Similarity indices and species turnover between first and second sampling for each method and double-sampled plot.................................................................................................................................... 19 Table 6. Results of mixed model analysis for effects of sampling method, vegetation type, response variable, and their interactions on log-transformed coefficient of variation. .......................................... 20 Tables 7 & 8. Means, standard deviations, coefficients