The Sensitizing and Indicator Action of Victoria Blue and Janus Green on the Flocculation Reaction for Syphilis
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Toxicological Evaluation of Certain Veterinary Drug Residues in Food
WHO FOOD ADDITIVES SERIES: 69 Prepared by the Seventy-eighth meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA) GENTIAN VIOLET page 3-34 Toxicological evaluation of certain veterinary drug residues in food , 2 7 The summaries and evaluations contained in this book are, in most cases, based on o. N unpublished proprietary data submitted for the purpose of the JECFA assessment. A registration es authority should not grant a registration on the basis of an evaluation unless it has first received i r e authorization for such use from the owner who submitted the data for JECFA review or has received the data on which the summaries are based, either from the owner of the data or from es es S a second party that has obtained permission from the owner of the data for this purpose. v i t i Add d World Health Organization, Geneva, 2014 oo F O O 6 1 H 0 W 2 GENTIAN VIOLET First draft prepared by Mr John Reeve 1 and Dr Susan Barlow 2 1 Science and Risk Assessment Branch, Ministry for Primary Industries, Wellington, New Zealand 2 Consultant, Brighton, East Sussex, England, United Kingdom 1. Explanation ........................................................................................... 4 2. Biological data ...................................................................................... 4 2.1 Biochemical aspects ....................................................................... 4 2.1.1 Absorption, distribution and excretion ................................... 4 (a) Mice ................................................................................ -
Identification of Typewriter Ribbons Charlotte L
Journal of Criminal Law and Criminology Volume 46 | Issue 6 Article 15 1956 Identification of Typewriter Ribbons Charlotte L. Brown Paul L. Kirk Follow this and additional works at: https://scholarlycommons.law.northwestern.edu/jclc Part of the Criminal Law Commons, Criminology Commons, and the Criminology and Criminal Justice Commons Recommended Citation Charlotte L. Brown, Paul L. Kirk, Identification of Typewriter Ribbons, 46 J. Crim. L. Criminology & Police Sci. 882 (1955-1956) This Criminology is brought to you for free and open access by Northwestern University School of Law Scholarly Commons. It has been accepted for inclusion in Journal of Criminal Law and Criminology by an authorized editor of Northwestern University School of Law Scholarly Commons. IDENTIFICATION OF TYPEWRITER RIBBONS* CHARLOTTE L. BROWN AND PAUL L. KIRK Mrs. Charlotte L. Brown, a member of the staff of the School of Criminology, Univer- sity of California, has collaborated with Dr. Kirk in the research and presentation of several articles that have appeared in this Journal during the last few years. Two of these, which appeared in volume 45, dealt with methods of identifying various types of writing inks. Paul L. Kirk is Professor of Criminology at the University of California and has con- tributed periodically to this Journal during the last fifteen years. He is the author of "Crime Investigation" and numerous articles on various laboratory techniques in several branches of criminalistics.-EDITOR. In the examination of typewritten documents it is frequently desirable to determine that a particular ribbon was used, that two or more documents were prepared with the same ribbon, or that more than one ribbon was used in preparing a single docu- ment. -
(12) United States Patent (10) Patent No.: US 6,905,539 B2 Patel Et Al
USOO6905539B2 (12) United States Patent (10) Patent No.: US 6,905,539 B2 Patel et al. (45) Date of Patent: Jun. 14, 2005 (54) BLACK ERADICABLE INK, METHODS OF 5,478,382. A 12/1995 Miller et al............... 106/22 B ERADICATION OF THE SAME, 5,486.228 A 1/1996 Miller et al. ..... ... 106/22 B ERADICABLE INK KIT, AND ERADICATED 5,489,331 A 2/1996 Miller et al. .............. 106/22 B INK COMPLEX 5,492,558 A 2/1996 Miller et al. .............. 106/22 B 5,498.282 A 3/1996 Miller et al. .. ... 106/22 B (75) Inventors: Sanjay Patel, Cypress, CA (US); David 5,498,285 A 3/1996 Hooykaas ................... 106/486 Godbout, Westmont, IL (US); Wing 5,499,881. A 3/1996 Chang........................ 401/17 Sum Vincent Kwan, Chicago, IL (US) E. A to: Eli - - - t; (73) Assignee: Sanford L.P., Freeport, IL (US) SEA GE Air O..."; (*) Notice: Subject to any disclaimer, the term of this SCA : R. S. G.O.". patent is extended or adjusted under 35 5.877234. A 3/1999 Xuetal... 523/161 U.S.C. 154(b) by 0 days. 5,916,357. A 6/1999 Wang et al. ............. 106/31.23 5,964,931 A 10/1999 Korper .................... 106/31.93 (21) Appl. No.: 10/619,706 5,977.211 A 11/1999 Koyama ..................... 523/161 5.997,891 A 12/1999 Fuerst et al. ................ 424/401 (22) Filed: Jul. 15, 2003 6,037,391 A 3/2000 Iida ............................ 523/161 6,048.914 A 4/2000 Goto et al. -
Student Safety Sheets Dyes, Stains & Indicators
Student safety sheets 70 Dyes, stains & indicators Substance Hazard Comment Solid dyes, stains & indicators including: DANGER: May include one or more of the following Acridine orange, Congo Red (Direct dye 28), Crystal violet statements: fatal/toxic if swallowed/in contact (methyl violet, Gentian Violet, Gram’s stain), Ethidium TOXIC HEALTH with skin/ if inhaled; causes severe skin burns & bromide, Malachite green (solvent green 1), Methyl eye damage/ serious eye damage; may cause orange, Nigrosin, Phenolphthalein, Rosaniline, Safranin allergy or asthma symptoms or breathing CORR. IRRIT. difficulties if inhaled; may cause genetic defects/ cancer/damage fertility or the unborn child; causes damages to organs/through prolonged or ENVIRONMENT repeated exposure. Solid dyes, stains & indicators including Alizarin (1,2- WARNING: May include one or more of the dihydroxyanthraquinone), Alizarin Red S, Aluminon (tri- following statements: harmful if swallowed/in ammonium aurine tricarboxylate), Aniline Blue (cotton / contact with skin/if inhaled; causes skin/serious spirit blue), Brilliant yellow, Cresol Red, DCPIP (2,6-dichl- eye irritation; may cause allergic skin reaction; orophenolindophenol, phenolindo-2,6-dichlorophenol, HEALTH suspected of causing genetic PIDCP), Direct Red 23, Disperse Yellow 7, Dithizone (di- defects/cancer/damaging fertility or the unborn phenylthiocarbazone), Eosin (Eosin Y), Eriochrome Black T child; may cause damage to organs/respiratory (Solochrome black), Fluorescein (& disodium salt), Haem- HARMFUL irritation/drowsiness or dizziness/damage to atoxylin, HHSNNA (Patton & Reeder’s indicator), Indigo, organs through prolonged or repeated exposure. Magenta (basic Fuchsin), May-Grunwald stain, Methyl- ene blue, Methyl green, Orcein, Phenol Red, Procion ENVIRON. dyes, Pyronin, Resazurin, Sudan I/II/IV dyes, Sudan black (Solvent Black 3), Thymol blue, Xylene cyanol FF Solid dyes, stains & indicators including Some dyes may contain hazardous impurities and Acid blue 40, Blue dextran, Bromocresol green, many have not been well researched. -
Gentian Violet S010
Gentian Violet S010 Gentian Violet is used as staining solution for monochrome staining of microbes. Composition** Ingredients Gentian violet 0.500 gm Distilled water 100.000 ml **Formula adjusted, standardized to suit performance parameters Directions 1) Prepare a smear on a clear, dry glass slide. 2) Allow it to air dry and fix with gentle heat. 3) Flood the slide with Gentian Violet (S010). 4) Allow the stain to be in contact with the smear for 1-2 minutes. 5) Wash in slow-running water, just enough to remove excess of dye. 6) Flood the smear with Iodine, drain and flood again with Iodine for 1 minute. 7) Wash with decolourizer (alcohol) for about 5-15 seconds. Wash the slide to stop the action of decolourizer. 8) Flood with safranin for 1 minute, wash very lightly. 9) Blot dry and examine under oil immersion objective. Principle And Interpretation Gentian Violet is used as a simple stain where it can render the organisms violet. Besides this it can also be used in the Gram staining for distinguishing between gram-positive and gram-negative organisms. Earlier, Gentian violet was used as the primary stain in Grams staining method, subsequently crystal violet has replaced gentian violet because of the defined chemical nature of crystal violet. Quality Control Appearance Dark purple coloured solution. Clarity Clear without any particles. Microscopic Examination Gram staining is carried out where Gentian Violet is used as one of the stains and staining characteristics of organisms are observed under microscope using oil immersion lens. Results Fq`l,onrhshudnqf`mhrlr9Uhnkds Fq`l,mdf`shudnqf`mhrlr9 Qdc Nsgdqdkdldmsr9U`qhntrrg`cdrneqdcsnotqokd Storage and Shelf Life Store below 30°C in tightly closed container and away from bright light. -
New Tetrachromic VOF Stain (Type III-G.S) for Normal and Pathological Fish Tissues C
ORIGINAL PAPER New Tetrachromic VOF Stain (Type III-G.S) for Normal and Pathological Fish Tissues C. Sarasquete,* M. Gutiérrez Instituto de Ciencias Marinas de Andalucía, CSIC Polígono Río San Pedro, Apdo oficial, Puerto Real, Cádiz, Spain richrome methods invariably use dyes in acid ©2005, European Journal of Histochemistry pH solvents, usually diluted in aqueous acetic Tacid, and the concentration of this acid A new VOF Type III-G.S stain was applied to histological sec- matches the concentration of dye. Staining depends tions of different organs and tissues of healthy and pathologi- largely on the attachment of dyes to proteins. The cal larvae, juvenile and adult fish species (Solea senegalensis; acid pH itself is necessary to maximise the amount Sparus aurata; Diplodus sargo; Pagrus auriga; Argyrosomus regius and Halobatrachus didactylus). In comparison to the of dye that will attach to tissue amino groups. original Gutiérrez´VOF stain, more acid dyes of contrasting Proteins have both positively (amino groups) and colours and polychromatic/metachromatic properties were negatively (carboxyl and hydroxyl) charged groups. incorporated as essential constituents of the tetrachromic VOF Usually one predominates and this will have an stain. This facilitates the selective staining of different basic tissues and improves the morphological analysis of histo- overall negative or positive charge (being an acid or chemical approaches of the cell components. The VOF-Type III a basic protein). These charges can, however, bal- G.S stain is composed of a mixture of several dyes of varying ance each other out to some degree. Phosphate size and molecular weight (Orange G< acid Fuchsin< Light green<Methyl Blue<Fast Green), which are used simultane- groups of DNA and binding-proteins are important ously, and it enables the individual tissues to be selectively dif- in nuclear staining.The ionisation of basic groups of ferentiated and stained. -
Colloid Milium: a Histochemical Study* James H
CORE Metadata, citation and similar papers at core.ac.uk Provided by Elsevier - Publisher Connector THE JOURNAL OF INVESTIOATIVE DERMATOLOOY vol. 49, No. 5 Copyright 1567 by The Williams & Wilkins Co. Printed in U.S.A. COLLOID MILIUM: A HISTOCHEMICAL STUDY* JAMES H. GRAHAM, M.D. AND ANTONIO S. MARQUES, M.D. Wagner (1), in 1866, first reported colloidreaction, with and without diastase digestion; milium in a 54 year old woman who showedcolloidal iron reaction, with and without bovine testicular hyaluronidase digestion for 1 hour at lesions on the forehead, cheeks and nose. In37 C; Movat's pentachrome I stain (2); alcian patients with colloid milium, the involvedblue pH 2.5 and 0.4 (3, 4); aldehyde-fuchsin pH skin is usually hyperpigmented, thickened,1.7 and 0.4 (4), with and without elastase digestion furrowed, nnd covered with multiple 0.5—5(5); Snook's reticulum stain; phosphotungstic acid hematoxylin stain (PTAH); Prussian blue re- mm dome-shaped, discrete papules. The shiny,action for iron; Fontana-Masson stain for ar- pink or orange to yellowish white translucentgentaffin granules; thiofiavine T fluorescent stain lesions have been likened to vesieles, but are(6, 7); Congo red; alkaline Congo red method firm and only after considerable pressure can(8); crystal violet amyloid stain; methyl violet a clear to yellow mueoid substance be ex-stain for amyloid (9, 5); toluidine blue (4); and Giemsa stain. The crystal violet and methyl vio- pressed from the papules. The lesions involvelet stained sections were mounted in Highman's sun exposed sites including the dorsum of theApathy gum syrup (5) which tends to prevent hands, web between the thumb and indexbleeding and gives a more permanent preparation. -
20 to 30 Sec)
457 Observations on a highly specific method for the histochemical detection of sulphated mucopolysaccharides, and its possible mechanisms By I. D. HEATH (From the Department of Anatomy, University of St. Andrews, Queen's College, Dundee. Present address: General Hospital, Nottingham) With 3 plates (figs, i to 3) Summary Whereas basic dyes in aqueous solutions stain chromatin, all mucins, mast cells, the ground substance of cartilage, and epidermis, it has been shown that a 0-03 % solution of basic dye in 5% aluminium sulphate produces a highly specific staining reaction for sulphated mucopolysaccharides. The best dyes are nuclear fast red (Herzberg) and methylene blue. Acid dyes in solutions of aluminium salts are induced to stain the ground substance of cartilage. These observations have been confirmed in a num- ber of species. Other metallic ions have similar properties and the use of green and purple chromic salts indicate that co-ordination plays a part in the reaction. Methylation, saponification, and sulphation experiments show that the sulphate group is essential. This has been confirmed by using pure chemical substances in gelatin models. Oxidation of keratin with performic acid, which produces sulphonic groups, causes hair (previously negative) to react. From this it is suggested that sul- phonic groups may also react, and that the reactive groups need not be attached to mucopolysaccharides. It is further suggested that the specificity of sulphated muco- polysaccharides is due to the fact that they are the only substances present in the tis- sues with a sufficient concentration of sulphate groups. Experiments with solochrome azurine show that the aluminium is attached to all tissue elements irrespective of their nature. -
Biological Stains & Dyes
BIOLOGICAL STAINS & DYES Developed for Biology, microbiology & industrial applications ACRIFLAVIN ALCIAN BLUE 8GX ACRIDINE ORANGE ALIZARINE CYANINE GREEN ANILINE BLUE (SPIRIT SOLUBLE) www.lobachemie.com BIOLOGICAL STAINS & DYES Staining is an important technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues. Loba Chemie offers comprehensive range of Stains and dyes, which are frequently used in Microbiology, Hematology, Histology, Cytology, Protein and DNA Staining after Electrophoresis and Fluorescence Microscopy etc. Many of our stains and dyes have specifications complying certified grade of Biological Stain Commission, and suitable for biological research. Stringent testing on all batches is performed to ensure consistency and satisfy necessary specification particularly in challenging work such as histology and molecular biology. Stains and dyes offer by Loba chemie includes Dry – powder form Stains and dyes as well as wet - ready to use solutions. Features: • Ideally suited to molecular biology or microbiology applications • Available in a wide range of innovative chemical packaging options. Range of Biological Stains & Dyes Product Code Product Name C.I. No CAS No 00590 ACRIDINE ORANGE 46005 10127-02-3 00600 ACRIFLAVIN 46000 8063-24-9 00830 ALCIAN BLUE 8GX 74240 33864-99-2 00840 ALIZARINE AR 58000 72-48-0 00852 ALIZARINE CYANINE GREEN 61570 4403-90-1 00980 AMARANTH 16185 915-67-3 01010 AMIDO BLACK 10B 20470 -
Mechanistic Insights Into Photodegradation of Organic Dyes Using Heterostructure Photocatalysts
catalysts Review Mechanistic Insights into Photodegradation of Organic Dyes Using Heterostructure Photocatalysts Yi-Hsuan Chiu 1 , Tso-Fu Mark Chang 2,*, Chun-Yi Chen 2,*, Masato Sone 2 and Yung-Jung Hsu 1,3,* 1 Department of Materials Science and Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan; [email protected] 2 Institute of Innovative Research, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8503, Japan; [email protected] 3 Center for Emergent Functional Matter Science, National Chiao Tung University, Hsinchu 30010, Taiwan * Correspondence: [email protected] (T.-F.M.C.); [email protected] (C.-Y.C.); [email protected] (Y.-J.H.) Received: 12 April 2019; Accepted: 3 May 2019; Published: 9 May 2019 Abstract: Due to its low cost, environmentally friendly process, and lack of secondary contamination, the photodegradation of dyes is regarded as a promising technology for industrial wastewater treatment. This technology demonstrates the light-enhanced generation of charge carriers and reactive radicals that non-selectively degrade various organic dyes into water, CO2, and other organic compounds via direct photodegradation or a sensitization-mediated degradation process. The overall efficiency of the photocatalysis system is closely dependent upon operational parameters that govern the adsorption and photodegradation of dye molecules, including the initial dye concentration, pH of the solution, temperature of the reaction medium, and light intensity.Additionally, the charge-carrier properties of the photocatalyst strongly affect the generation of reactive species in the heterogeneous photodegradation and thereby dictate the photodegradation efficiency. -
A Comparative Study of Staining and Histochemical Reactions of the Components of Golgi Complex of the Fibroblasts in Vitro and the Ascites Tumor Cells
448 A Comparative Study of Staining and Histochemical Reactions of the Components of Golgi Complex of the Fibroblasts in vitro and the Ascites Tumor Cells Shunzo Takagi, Jin-ichi Kitada, Hideo Masuda and Masayuki Tagawa Departmentof Biology,University of OsakaPrefecture, Sakai,Japan ReceivedJune 27, 1961 It was previously shown with phase optics that the Golgi apparatus in living cells cultured in vitro, such as fibroblasts, corneal epithelial cells, myoblasts and white cells of the domestic fowl, consists of canalicules, which are usually seen as vacuoles in optical section, and of delicate filaments in a juxtanuclear area distinguishable from the rest of the cytoplasm (Takagi and Masuda 1956, Takagi 1958). Similar canalicules and filaments were also found to constitute the Golgi apparatus of frog lymphocytes (Takagi and Tagawa 1957). The walls of canalicules and the filaments are readily black ened by osmium tetroxide and the whole apparatus appears like a congeries of black strands. They are also stained vitally with Janus green and neutral red, and vitally and metachromatically with methylene blue, trimethylthionin and toluidine blue. The vital staining indicates that a canalicule is a dilated filament or often a dilated part of a filament. A number of electron microscopists have revealed that the Golgi complex consists of vacuoles, smooth membranes and small vesicles or granules, and the vacuoles are a dilatation of a double membrane, being characteristic of an actively secreting or absorbing cell (Haguenau and Bernhard 1955, Gatenby and Lufty 1956, Sjostrand 1956, Dalton and Felix 1956, 1957, Lacy 1957). The canalicules and the filaments observable in the living cells under the phase microscope may in all probability correspond to the vacuoles and the smooth membranes respectively in the electron micrographs, in which the latter often appear in the form of flattened vesicles and tubules. -
Discontinued List to Go on Website
Animal Serum Code Description Size Price £ G210 Calf (Aseptic Donor) 100ml 13.90 G250 Calf (Aseptic Donor) 500ml 38.60 G212 Calf (Newborn) - under 14 days old 100ml 5.65 G252 Calf (Newborn) - under 14 days old 500ml 22.30 G610 Horse (Natural Clot) 100ml 7.80 G650 Horse (Natural Clot) 500ml 31.90 Multitest Slides Code Wells Size (mm) 61100-00 1 8 61100-01 3 8 61100-04 3 14 61100-05 8 6 61100-09 10 8 61100-10 2 10 61100-11 10 5 61100-12 36 2 61100-14 10 6 61100-16 10 7 61100-18 4 14 61100-19 12 8 61100-24 1 18 61100-25 15 4 61100-26 14 5 61100-28 21 4 61100-29 12 6 61100-30 3 11 61100-33 12 3 61100-36 18 5 61100-50 30 3 61100-60 4 6 Buffers and Solutions Code Description Size Price £ HDS15/25 Sorensen's Buffer pH 6.5 (1 vial makes 5L) 25 vials 35.00 HDS20/25 Sorensen's Buffer pH 7.0-7.6 (1 vial makes 5L)* 25 vials 36.75 HDS25 PBS for Osmotic Fragility (makes 250ml) 25ml 2.65 HDS35 PBS pH 7.2 or 7.6 for Immunofluorescence (makes 2L) 100ml 3.40 Immunocytochemistry Substrate Tablets Code Description Pack Price £ HD4190 AEC Effervescent Buffered pH 5.1 5mg x 50 44.80 HD4170 DAB Effervescent Buffered pH 7.0 10mg x 50 63.10 HD4240 DAB Unbuffered 10mg x 50 55.20 HD4120 Fast Red Standard Unbuffered 2mg x 50 47.90 HD4360 Ureaperoxide 5.68mg x 50 65.80 Embedding and Mounting Media, Fixatives and Chemicals Code Description Size Price £ Size Price £ HC8503 Acetone 2L 4.25 HC8510 Acacia Powder 100g 5.35 HC8520 Aluminium Sulphate 500g 4.20 HC8530 Aniline Xylene 500ml 11.15 HC8540 Beeswax - White 100g 4.10 500g 14.30 HC8542 Berlese Fluid (gum chloral)