FAST TOXICOLOGICAL SCREENING KIT BY LC-MS/MS – Code SC9005B

The kit for the toxicologicalforensic analysis is a screening method to analyze hundreds of compounds in positive and negative mode, in plasma and blood. It is possible to determine the following molecles:

POSITIVE MODE: Amitrole Alypin Bamifylline 17 alpha Methyltestosterone Amantadine Bamipin 2Amino 5chlorobenzophenone Ambroxol Beclamide 2Amino 5nitrobenzophenone Ambucetamide Beclometasone dipropionate 2Hydroxyethylflurazepam Amezinium Befunolol 3,4 Methylenedioxyamphetamine Amidopyrin Bendiocarb 3,4 Methylenedioxyethylamphetamine Amidotrizoic Acid Benfluorex 3,4 Methylenedioxymethamphet amine Amiloride Benodanil 3,5 Diiodotyrosine Aminodantrolene Benperidole 4Benzamidosalicyclic acid Aminoglutethimide Benproperine 6Mercaptourine Aminophenazone Bens l tap 6OMonoacetylmorphine Aminopromazine Bentiromide 7Aminoclonazepam Aminorex Benzatropine 7Aminodesmethylflunitrazepam Amiodarone Benzocaine 7Aminoflunitrazepam Amiphenazole Benzoct amine 7Aminonitrazepam Amis l pride Benzododecinium 9Hydroxyrisperidone Amitriptylin Benzoylecgonine cebutolol Amlodipine Benzthiazuron Aceclidine Ammoidin Berberine Aceclofenac Amorolfine Betamethasone 21 phosphate Acemetacin Amoxicillin Betaxolol Acep romazine Amphetamine Bevonium Aceprometazine Amrinone Bezafibrate Acetiamin Antazoline Biperiden Acetylaminonitroprophoxybenzene Apalcillin Bisoprolol Aciclovir Apomorphine Bornaprine Actinoquinol Apophedrin Brodifacoum Adenine Apraclonidin Bromacil Adrenalone Aprinidine Bromazepam Ajmaline Atenolol Bromocriptine Alachlor Atorvastatin Brompheniramine Alclometasone 17,21 dipropionate Atraton Brotizolam Alimemazine Atrazine desethyl Bucetin Alizapride Atropine Bufe xamac Allopurinol Atropinmethylbromid Bumetanide Almitrine Az atadi ne Bunazosin alpha Hydroxyalprazolam Azelastine Bunitrolol alpha Hydrox ytriazolam Azintamid Bupivacaine Alprazolam Aztreonam Bupranolol Alprenolol Baclofen Buprenorphine Altretamine Bambuterol Buprofezin

1 Buspirone Clobenzepam Dienogest Butaperazine Clobutino l Diethazine Butetamate Cl omethiazole Diethylcarbamazine Butoxycaine Clomipramine Difenoconazole Cafaminol Clonazepam Difenoxuron Caffeine Clonidine Diflucortolone Candesartan Clopenthixol Dihydrocodeine Caproylresorcinol Clopidogrel Dihydroergocristin Captopril Cloprednol Dihydr oergotamine Carazolol Clozapine Dilazep Carbamazepine Cocaine Diltiazem Carbamazepine 10,11 ep oxide Codeine Dimetindene Carbendazim Coniine Dimetotiazine Carbinoxamine Corticosterone Dimetridazole Carbutamide Cortisone Dioxethedrin Carbuterol Cotinine Diphenamid Carteolol Coumatetralyl Diphenhydramine Carvedilol Croconazole Diponium Celiprolol Cromoglicic acid Diprophylline Cerivastatin Cyamemazine Dipyridamole Cetirizine Cyclicine Disopyramide Chinine Cyclophosphamide Dixyrazine Chlorazanil Cycl ovalone Dobutamine Chlorbenzoxamine Cyproheptadine Dorzolamide Chlorcyclizine Cytarabine Doxapram Chlordiazepoxide D3 Doxepin Doxepin Chloridazon D5 Diazepam Doxylamine Chlormadinone Acetate Dapiprazole Drofenine Chlormequat Deflazacort Dummy Chlorpheneth iazin e Demeton Smethyls l fone Ecgoninemethylester Chlorpheniramine Denaverine EDDP Chlorpromazine Desalkylflurazepam Embutramide Chlorpromazine S l foxide Desipramine Enalapril Chlorprothixene Desmedipham Enoximone Cilastatin Desmethylclobazam Ephedrine Cilazapril Desmethylclomipramine Eprosartan Cimetidine Dexamethasone Esc l in Cinnarizine Dexamethasone 21 isonic otina te Esmolol Cinoxacin Dexfenfluramine Estazolam Ciprofloxacin Dextromethorphan Ethenzamide Cisapride Dextropropoxyphene Etofenamate Citalopram Diaveridine Etomidate Clarithromycin Diazepam Etoposide Clemastine Dibutyl adipate Famotidine Clenbuterol Diclofenac Fedrilate Clobazam Dicycloverine Felbamate

2 Felodipine Guanoxan Lisinopril Fenarimol Haloperidol Lonazolac Fendiline Haloxyfop ethoxyethyl ester Loperamide Fenetylline Heptaminol Loratadine Fenflu ramin e Heroin Lorazepam Fenpipramide Hexazinone Lormetazepam Fenpiprane Hexobendine Losartan Fenproporex Histamine Loxapine Fentanyl Histapyrrodine Lysergide Fenticonazole Homatropine Mafenide Fexofenadine Hordenine Mapro tilin e Flecainide Hydrocodone MBDB Flocoumafen Hydrocortisone Mebeverine Floctafenine Hydrocortisone 21 acetate Meclizine Fluanisone Hydromorphone Meclofenamic acid Fluconazole Hydr oxyzine Mecloxamine Flumazenil Hymecromone Meclozine Flunarizine Imipramine Medazepam Flunitrazepam Indanazoline Mefenamic acid Fluoxetine Indapamine Mefenorex Flupentixol Indinavir Mefexamide Fluphenazine Indoprofen Mefloquine Flupirtine Indoramin Melatonin Flurazepam Iopodic acid Melitracen Flu rochl oridone Ipratropium Meloxicam Fluspirilen Iprazochrome Melperone Fluticasone Propionate Irbesartan Melphalan Fluvastadine Isoaminile Mepindolol Fluvoxamine Isoconazole Mepivacaine Fuberidazole Isoprenaline Meptazinol Furalaxyl Isoproturon Mequitazine Gabapentin Isothipendyl Mescalin e Galantamine Isoxicam Me soridazine Gallopamil Isoxsuprine Mesuximide Gemcitabine Kavain Metaclazepam Glibenclamide Ketamin e Metamfepramone Glibornuride Ke toprofen Metamitron Gliclazide Ketorolac Metamphetamine Glimepiride Ketotifen Metazachlor Glipizide Lamotrigine Metenolone acetate Gliquidone Lercanidipine Metformin Griseof l vin Levocabastine Methabenzthiazuron Guaifenesin Levodopa Methadone Guan abenz Levomepromazine Methaphenilene Guanethidine Levopropylhexedrin Methapyrilene Guanfacine Lidocaine Methaqualon

3 Methazolamide Nebivolol Oxilofrine Methfuroxam Nefopam Oxitriptan Methocarbamol Nicardipine Oxitropium Methopr otryn e Nicotinamide Oxomemazine Methotrexate Nicotine Oxprenolol Methylephedrine Nifedipine Oxybuprocaine Methylphenidate Nifenazone Oxybutynin Methylscopolamine Niflumic acid Oxyco done Methylthiouracil Nimodipine Oxyfedrine Metipranolol Nimorazole Oxymetazoline Metixene Nisoldipine Oxymorphon Metoprolol NIsopropylsalicylamide Oxypertine Metronidazole Nitrazepam Papaverine Mets l furon methyl Nitrendipine Paracetamol Metyrapone Nizatidi ne Paraoxon Mexiletine NMethylephedrine Paroxetine Mianserin Nomifensine Penfluridol Miconazole Norbuprenorphine Pentamidine Midazolam Nordiazepam Pentoxyverine Midodrine Nordiazepam N ethyl Perazine Milrinone Norephedrine Periciazine Minoxidil Norethisterone Perindopril Mirtaz apine Norethisterone acetate Perphenazine Mizolastine Norfenefrine Pethidine Moclobemide Norfentanyl Phenacetin Modafinil Norfloxacin Phenazone Molsidomine Normorphine Phenazopyridine Monocrotophos Nortriptyline Phencyclid ine Monolinuron Noscapine Phenelzine Moperone Nuarimol Pheniramine Morphine Obidoxime Phenprocoumon Morphine 3ßDglucuronide Ofloxacin Phenylephrine Moxaverine Ola nzapi ne Phenyltoloxamine Moxisylyte Ondansetron Phenytoin Moxonidine Opipramol Pholedrine Nabumetone Orciprenaline Physostigmine Nadolol Ornidazole Pilocarpine Naftidrofuryl Orphenadrine Pindolol Naftifine Oxadixyl Pioglitazone Nalidixic acid Oxamyl Pipamperone Nalorphine Oxatomide Pirbuterol Naloxon e Oxazepam Pirenzepine Na ltrexone Oxcarbazepine Piretanide Nandrolone Oxeladin Piritramide Naphazoline Oxetacaine Piroxicam NDespropylpropafenone Oxiconazole Pizotifen

4 Practolol Risperidone Tetroxoprim Prajmal ium Ritodrine Tetryzoline Pramipexole Rizatriptan THC Prazepam Ropinirole THC COOH Prazosin Ropivacaine THC OH Prednisolone Rosiglitazon Thebacon Prednisone Salbutamol Theobromine Prilocaine Salicylamide Theophylline Primaquine Scopolamine Thiabendazole Primidone Serotonin Thiamazole Procainamide Sertindole Thiamine Prochlorperazine Sertraline Thiopr opaza te Procyclidine Sildenafil Thioproperazine Progesterone Simazine Thioridazine Promazine Sotalol Thiram Promethazin Spirapril Tiagabine Prometryn Stano zolol Tiapride Propafenone Sl fabenzamide Ticlopidine Propiconazole Sl fadiazine Tiemonium Propionylpromazine Sl fadoxine Tilidine Propipocaine Sl faethidole Timolol Propi verine Sl faguanidine Tinidazole Propranolol Sl famethizole Tizanidine Propyphenazone Sl famethoxazole Tocainide Prothiopendyl Sl fapyridine Tolazoline Protriptyline Sl faquinoxaline Tolbutamide Pseudoephedrine Sl indac Toliprolol Psilocin Sl piride Tolnaftate Pyranocoumarin Sl tiame Tolpropamine Pyribenzamine Sumatriptan Tolycaine Pyridoxine Suxibuzone Topotecan Pyrimethamine Tadalafil Torasemide Pyritinol Talinolol Tramadol Pyrvinium Tamoxifen Tr anexa mic acid Quetiapine Telmisartan Trazodone Quinapril Temaze pam Triadimenol Quinine Tenocixam Triallate Ramipril Terazosine Triamterene Ranitidine Terbinafine Triazolam Raubasine Terbutaline Trifluperazine Reboxetine Terbutryn Trifluperidol Remox ipride Terconazole Triflupromazine Repaglinide Terfenadine Trimethobenzamide Reproterol Tertalolol Trimethoprim Reserpine Tetracaine Trimipramine Riluzole Tetrazepam Tripelennamine

5 Triprolidine Valdecoxib Xipamide Tritoqualine Valsartan Xylometazoline Tromantadine Vardenafil Yohimbine Tropisetron Venlafaxine Zolpidem Trospium Verapamil Zopiclone Trypta mine Vincamine Zuclopenthixol Tl obuterol Warfarin Urapidil Xantinol

NEGATIVE MODE: Azelaic Acid Ethyl s l fate 2Amino 5nitrobenzophenone Aztreonam Ethyl s l fate D5 4Ben zamidosalicyclic acid Benserazid Furosemid 5(p Methylphenyl) phenylhydantoin Benzthiazide Hydrochlorothiazide 5Aminosalicylic acid Bezafibrate Morphin 3ßD glucuronide D3 6Mercaptourine Brallobarbital Pentobarbital Acetazolamide Carbenoxolone Phenobarbital Acetylsalicylic Acid Chlorothiazide Propallylonal Adenine Cimetidine THC COOH Adenosine Cinchonine THC OH Adrenalone Cortisone Triamcinolone Alprostadil Dinoprost Amobarbital Epine phrin e Amoxicillin Ethyl glucuronide Atorvastatin Ethyl glucuronide D5

6 FAST TOXICOLOGICAL SCREENING KIT – Code SC9005B

TECHNICAL FEATURES

Principle of the Method:

After optimizing the retention times of the analytes by means of the injection of the Mix Standard in LC MS/MS, it will be possible to inject the deproteinized plasma / blood to determine intoxication by unknown molecles.

Components of the kit (500 tests): All reagents are ready to use and stable 3 years at 2–8 °C.

Reagent A – Deproteinization Solution with internal Standard, 1 x 150 ml

Reagent E – Stabilizing Solution, 1 x 25 ml

Reagent F – Standard mix Solution, 1 x 3 ml

Reagent M1 – Mobile Phase M1, 20 x 500 ml

Reagent M2 – Mobile Phase M2, 20 x 500 ml

Minimum Instrumental equipment: ® Instrument API 3200 QTRAP Instrument API 4000 – 4500 QTRAP ® ® Instrument API 5500 QTRAP Operational Computer Optional equipment: Autosampler

Blood Collection Procedure: Tube with con anticoaglant EDTA Stable for about 4 weeks at 2 – 8 °C or 4 months at –20°C.

EUREKA srl – LAB DIVISION Head Quarter: VAT N° 01547310423 Via Enrico Fermi 25 E-mail:[email protected] 60033 Chiaravalle (AN) ITALY www.eurekaone.com Tel. +39 071 7450790 Fax + 39 071 7496579

This product meets all the requirements of Directive 98/79 / EC on in vitro diagnostic medical devices (IVD). The EC declaration of conformity is available on request.

Release N° 00 1 Fast To xicolog ical Screening Kit April 2019

7 PREANALYTICAL PHASE

PREPARATION OF STANDARD MIX FOR SYSTEM SET UP:

1) dilute 1 at 10 in Methanol the Reagent F – Standard mix Solution.

2) Prepare the following dilutions using the Reagent M1 – Mobile Phase M1. ® • For API 3200 QTRAP systems: add 100 l of solution diluted at step 1 and add 900 L of Reagent M1 – Mobile Phase M1 • For API 4000 4500 QTRAP® systems: add 10 l of solution diluted at step 1 and add 90 l of Methanol and 900 L of Reagent M1 – Mobile Phase M1 ® • API 5500 QTRAP systems: add 2 l of solution diluted at step 1 and add 98 l of Methanol and 900 L of Reagent M1 – Mobile Phase M1

INJECTION :

• Inject 10 30 l

This standard shold be injected at the beginning of each session as a System Suitability Test to check the instrumental and chromatographic performance.

ANALYTICAL PROCEDURE

STEP 1 : In eppendorf dispense in sequence:

• 100 l of SAMPLES + 300 l of Reagent A – Deproteinization Solution with Internal Standard

STEP 2 : centrifuge at 10.000 rpm for 10 minutes

STEP 3 : dilute 100 l of surnatant with 100 l of Reagent E – Stabilizing Solution

INJECTION :

• Inject 30 l

Release N° 00 1 Fast Toxicological Screening Kit April 2019

8

FAST TOXICOLOGICAL SCREENING KIT – Warnings

(THIS KIT HAS BEEN DEVELOP FOR SCIEX LC/MS SYSTEMS QTRAP CONFIGURATION)

INSTRUMENTAL CONDITIONS: Cromatographic Gradient

Gradient Profile Time (min) Flow Rate A (%) B (%) (µL/min) 0.00 500 90 10 10.00 1000 10 90 15.00 1000 10 90 15.50 500 90 10 18.00 500 90 10

Flow rate: 0.5 mL/min Column oven temperature: 40°C

Separation Column Drug Screen and Quant Analytical Column, 5 m 60 Å, PFP Propyl Column, 50 x 2.1 mm (P/N 9169552)

CODE ACCESSORIES DESCRIPTION PACKAGING Drug Screen and Quant Analytical Column, 5 m 60 SRE9169552 1 PK Å, PFP Propyl Column, 50 x 2.1 mm SK9001 PEEK Tubing O.D. × I.D. 1/16 in. × 0.005 in., red 1 PK PEEK Tubing O.D. × I.D. 1/16 in. × 0.010 in., blue Peek fitting Onepiece fingertight F130X S90199511 Prefilters Javelin 1 x 10 PK

9 FAST TOXICOLOGICAL SCREENING KIT (Reference Chromatograms/Spectra)

10 FAST TOXICOLOGICAL SCREENING KIT (Reference Chromatograms/Spectra)

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