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Acari: Ixodida: Argasidae), with Sequence Information from the Mitochondrial 16S Rdna Gene S Morphological study of Ornithodoros viguerasi cooley and kohls, 1941 (Acari: Ixodida: Argasidae), with sequence information from the mitochondrial 16S rDNA gene S. Nava, J.M. Venzal, E.A. Reyes Novelo, A.J. Mangold, M.B. Labruna To cite this version: S. Nava, J.M. Venzal, E.A. Reyes Novelo, A.J. Mangold, M.B. Labruna. Morphological study of Or- nithodoros viguerasi cooley and kohls, 1941 (Acari: Ixodida: Argasidae), with sequence information from the mitochondrial 16S rDNA gene. Acarologia, Acarologia, 2012, 52 (1), pp.29-38. 10.1051/ac- arologia/20122035. hal-01566535 HAL Id: hal-01566535 https://hal.archives-ouvertes.fr/hal-01566535 Submitted on 21 Jul 2017 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution - ShareAlike| 4.0 International License ACAROLOGIA A quarterly journal of acarology, since 1959 Publishing on all aspects of the Acari All information: http://www1.montpellier.inra.fr/CBGP/acarologia/ [email protected] Acarologia is proudly non-profit, with no page charges and free open access Please help us maintain this system by encouraging your institutes to subscribe to the print version of the journal and by sending us your high quality research on the Acari. Subscriptions: Year 2017 (Volume 57): 380 € http://www1.montpellier.inra.fr/CBGP/acarologia/subscribe.php Previous volumes (2010-2015): 250 € / year (4 issues) Acarologia, CBGP, CS 30016, 34988 MONTFERRIER-sur-LEZ Cedex, France The digitalization of Acarologia papers prior to 2000 was supported by Agropolis Fondation under the reference ID 1500-024 through the « Investissements d’avenir » programme (Labex Agro: ANR-10-LABX-0001-01) Acarologia is under free license and distributed under the terms of the Creative Commons-BY-NC-ND which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. Acarologia 52(1): 29–38 (2012) DOI: 10.1051/acarologia/20122035 MORPHOLOGICAL STUDY OF ORNITHODOROS VIGUERASI COOLEY AND KOHLS, 1941 (ACARI: IXODIDA: ARGASIDAE), WITH SEQUENCE INFORMATION FROM THE MITOCHONDRIAL 16S rDNA GENE Santiago NAVA1, José M. VENZAL2, Enrique A. REYES NOVELO3, Atilio J. MANGOLD1 and Marcelo B. LABRUNA4 (Received 16 November 2011; accepted 16 January 2012; published online 30 March 2012) 1 Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Rafaela and Consejo Nacional de Investigaciones Científicas y Técnicas, CC 22, CP 2300 Rafaela, Santa Fe, Argentina. [email protected]. [email protected] 2 Departamento de Parasitología Veterinaria, Facultad de Veterinaria, Universidad de la República, Regional Norte – Sede Salto, Rivera 1350, CP 50000 Salto, Uruguay. [email protected] 3 Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, CP 97000, Mérida, Yucatán, México. [email protected] 4 Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Av. Prof. Orlando M. de Paiva 87, 05508-900, São Paulo, Brazil. [email protected] ABSTRACT — A morphological and molecular study of Ornithodoros (Subparmatus) viguerasi (Acari: Argasidae) was carried out. Free-living males and females of this tick species were collected in Calcehtok cavern, Yucatán, Mexico. The morphology of the females of O. viguerasi was identical to the holotype female from Cuba. The only difference was related to size; females from Mexico were bigger than the holotype female. The male of O. viguerasi was described for the first time from the specimens collected in Mexico. The diagnostic characters are a combination of a genital aperture covered by a semicircular flap, a central sclerotized plate posterior to genital aperture, a transverse and thin plate anterior to the genital aperture located at the level of the anterior margin of coxae I, a pair of sclerotized plates bordering coxae II, III and IV, basis capituli rectangular in shape and protrusible, a hypostome roughly blunt at the apex with small denticles (it appears to be functionless), and article I of the palpi with a medial integumental extension which has a long setae on the medial margin. A comparative morphological analysis of the male described in this work with the paratype specimens from Cuba was also conducted; we conclude that the nymphs described in the original description of O. viguerasi correspond to males. Sequences of the mitochondrial 16S rDNA gene of both male and female ticks from Mexico were identical. In a phylogenetic analysis, the monophyly of the subgenus Subparmatus could not be resolved. KEYWORDS — Ornithodoros viguerasi; Argasidae; morphology; 16S rDNA; Mexico INTRODUCTION Kohls, Clifford and Jones, 1969 and Ornithodoros mormoops Kohls, Clifford and Jones, 1969 (Kohls The argasid tick Ornithodoros viguerasi Cooley and et al. 1969). The geographical distribution of Kohls, 1941 was described from specimens collected O. viguerasi includes Cuba, Costa Rica, Domini- from a bat cave in Cuba. This species belongs to the can Republic, Haiti, Jamaica, Mexico, Puerto Rico, subgenus Subparmatus with Ornithodoros marinkellei http://www1.montpellier.inra.fr/CBGP/acarologia/ 29 ISSN 0044-586-X (print). ISSN 2107-7207 (electronic) Nava S. et al. Trinidad & Tobago and Venezuela (Tamsitt and Fox MATERIALS AND METHODS 1970; Jones et al. 1972; Hoogstraal 1985; Gugliel- Free-living argasid ticks (males and females) were mone et al. 2003). All previous records of host- collected by the authors during June 2010 in Cal- tick associations for O. viguerasi correspond to lar- cehtok cavern (20º33´02´´N, 89º54´43´´W), Yucatán, vae parasitizing bats: larvae have been collected Mexico. After morphological analysis, 16 females on Phyllonycteris poeyi Gundlach, 1861; Pteronotus were identified as O. viguerasi following Cooley and macleayii Gray, 1839; Pteronotus quadridens Gund- Kohls (1941). Seventeen males were tentatively as- lach, 1840; Pteronotus parnellii Gray, 1843; Pterono- signed to O. viguerasi due to the presence of scle- tus gymnonotus Natterer, 1843; Pteronotus davyi Gray, rotized ventral plates, which is a diagnostic char- 1838; Brachyphylla nana Miller, 1902; Mormoops mega- acter of adults of the subgenus Subparmatus. Ad- lophylla Peters, 1864; Mormoops blainvillei Leach, ditionally, a fragment of circa 420-bp of the mito- 1821; Erophylla sezekorni Gundlach, 1861; and Ero- chondrial 16S rDNA gene was obtained from two phylla bombifrons Miller, 1899 (Kohls et al. 1965; males and one female in order to confirm the tax- Cerný and Dusbábek 1967; Cerný 1969; Tamsitt and onomic determination by morphological criteria. Fox 1970; Jones et al. 1972; De la Cruz and Abreu Both males and females were deposited at the Tick 1984; Kurta et al. 2007). Collection of the Instituto Nacional de Tecnología The description of Cooley and Kohls (1941) was Agropecuaria, Estación Experimental Agropecuaria based on a female, nymphs and larvae collected in Rafaela, Rafaela, Santa Fe, Argentina (INTA 2187), "Cueva Somorrostro", Cuba. Larvae were found at- at the Coleção Nacional de Carrapatos (CNC) of tached to bats, probably P. poeyi. Although Gugliel- the Faculdade de Medicina Veterinária e Zootecnia, mone et al. (2003) stated that all stages of O. viguerasi Universidade de São Paulo, São Paulo, Brazil (CNC were described, the description of adults by Coo- 1717) and in the Departamento de Parasitología Vet- ley and Kohls (1941) was based on only the female, erinaria, Facultad de Veterinaria, Universidad de la without inclusion of male specimens. Danielová et República, Montevideo, Uruguay (DPVURU 768). al. (1982) reported the finding of males and females Six males and six females were measured using of O. viguerasi on the walls of bat caves in Cuba, but a stereoscope Nikon® C-PS (all measurements are the methodology for tick determination was not ex- given in mm, the mean followed by the range in plained by these authors and the collection where parentheses). Scanning electron photomicrographs the ticks were deposited was not indicated. There- of males and females were taken at the Servicio fore, it can be affirmed that the male of this tick de Microscopía Electrónica, Museo de La Plata, species is not formally described. Universidad Nacional de La Plata, Argentina, us- The aim of this work was to carry out the de- ing a JEOL/JSM 6360 LV® Digital Scanning Mi- scription of males of O. viguerasi from specimens re- croscope, and Sub-Unidad de Microscopía Elec- cently collected from a bat cave in Mexico, and to trónica de Barrido, Facultad de Ciencias, Montev- compare the morphology of both males and females ideo, Uruguay, using JEOL JMS-5900 scanning elec- from Mexico with the morphology of the specimens tron microscope. The holotype female (RML17169), of O. viguerasi described by Cooley and Kohls (1941) the paratype nymph (RML17164) and other mate- and also with those deposited in the United States rial (RML50482, RML64683, RML17495, RML64680, National Tick Collection (USNTC, Georgia South- RML64679, RML64682, RML52459, RML17162,
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