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Morphological study of Ornithodoros viguerasi cooley and kohls, 1941 (Acari: Ixodida: Argasidae), with sequence information from the mitochondrial 16S rDNA gene S. Nava, J.M. Venzal, E.A. Reyes Novelo, A.J. Mangold, M.B. Labruna To cite this version: S. Nava, J.M. Venzal, E.A. Reyes Novelo, A.J. Mangold, M.B. Labruna. Morphological study of Or- nithodoros viguerasi cooley and kohls, 1941 (Acari: Ixodida: Argasidae), with sequence information from the mitochondrial 16S rDNA gene. Acarologia, Acarologia, 2012, 52 (1), pp.29-38. 10.1051/ac- arologia/20122035. hal-01566535 HAL Id: hal-01566535 https://hal.archives-ouvertes.fr/hal-01566535 Submitted on 21 Jul 2017 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution - ShareAlike| 4.0 International License ACAROLOGIA A quarterly journal of acarology, since 1959 Publishing on all aspects of the Acari All information: http://www1.montpellier.inra.fr/CBGP/acarologia/ [email protected] Acarologia is proudly non-profit, with no page charges and free open access Please help us maintain this system by encouraging your institutes to subscribe to the print version of the journal and by sending us your high quality research on the Acari. Subscriptions: Year 2017 (Volume 57): 380 € http://www1.montpellier.inra.fr/CBGP/acarologia/subscribe.php Previous volumes (2010-2015): 250 € / year (4 issues) Acarologia, CBGP, CS 30016, 34988 MONTFERRIER-sur-LEZ Cedex, France The digitalization of Acarologia papers prior to 2000 was supported by Agropolis Fondation under the reference ID 1500-024 through the « Investissements d’avenir » programme (Labex Agro: ANR-10-LABX-0001-01) Acarologia is under free license and distributed under the terms of the Creative Commons-BY-NC-ND which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. Acarologia 52(1): 29–38 (2012) DOI: 10.1051/acarologia/20122035 MORPHOLOGICAL STUDY OF ORNITHODOROS VIGUERASI COOLEY AND KOHLS, 1941 (ACARI: IXODIDA: ARGASIDAE), WITH SEQUENCE INFORMATION FROM THE MITOCHONDRIAL 16S rDNA GENE Santiago NAVA1, José M. VENZAL2, Enrique A. REYES NOVELO3, Atilio J. MANGOLD1 and Marcelo B. LABRUNA4 (Received 16 November 2011; accepted 16 January 2012; published online 30 March 2012) 1 Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Rafaela and Consejo Nacional de Investigaciones Científicas y Técnicas, CC 22, CP 2300 Rafaela, Santa Fe, Argentina. [email protected]. [email protected] 2 Departamento de Parasitología Veterinaria, Facultad de Veterinaria, Universidad de la República, Regional Norte – Sede Salto, Rivera 1350, CP 50000 Salto, Uruguay. [email protected] 3 Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, CP 97000, Mérida, Yucatán, México. [email protected] 4 Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Av. Prof. Orlando M. de Paiva 87, 05508-900, São Paulo, Brazil. [email protected] ABSTRACT — A morphological and molecular study of Ornithodoros (Subparmatus) viguerasi (Acari: Argasidae) was carried out. Free-living males and females of this tick species were collected in Calcehtok cavern, Yucatán, Mexico. The morphology of the females of O. viguerasi was identical to the holotype female from Cuba. The only difference was related to size; females from Mexico were bigger than the holotype female. The male of O. viguerasi was described for the first time from the specimens collected in Mexico. The diagnostic characters are a combination of a genital aperture covered by a semicircular flap, a central sclerotized plate posterior to genital aperture, a transverse and thin plate anterior to the genital aperture located at the level of the anterior margin of coxae I, a pair of sclerotized plates bordering coxae II, III and IV, basis capituli rectangular in shape and protrusible, a hypostome roughly blunt at the apex with small denticles (it appears to be functionless), and article I of the palpi with a medial integumental extension which has a long setae on the medial margin. A comparative morphological analysis of the male described in this work with the paratype specimens from Cuba was also conducted; we conclude that the nymphs described in the original description of O. viguerasi correspond to males. Sequences of the mitochondrial 16S rDNA gene of both male and female ticks from Mexico were identical. In a phylogenetic analysis, the monophyly of the subgenus Subparmatus could not be resolved. KEYWORDS — Ornithodoros viguerasi; Argasidae; morphology; 16S rDNA; Mexico INTRODUCTION Kohls, Clifford and Jones, 1969 and Ornithodoros mormoops Kohls, Clifford and Jones, 1969 (Kohls The argasid tick Ornithodoros viguerasi Cooley and et al. 1969). The geographical distribution of Kohls, 1941 was described from specimens collected O. viguerasi includes Cuba, Costa Rica, Domini- from a bat cave in Cuba. This species belongs to the can Republic, Haiti, Jamaica, Mexico, Puerto Rico, subgenus Subparmatus with Ornithodoros marinkellei http://www1.montpellier.inra.fr/CBGP/acarologia/ 29 ISSN 0044-586-X (print). ISSN 2107-7207 (electronic) Nava S. et al. Trinidad & Tobago and Venezuela (Tamsitt and Fox MATERIALS AND METHODS 1970; Jones et al. 1972; Hoogstraal 1985; Gugliel- Free-living argasid ticks (males and females) were mone et al. 2003). All previous records of host- collected by the authors during June 2010 in Cal- tick associations for O. viguerasi correspond to lar- cehtok cavern (20º33´02´´N, 89º54´43´´W), Yucatán, vae parasitizing bats: larvae have been collected Mexico. After morphological analysis, 16 females on Phyllonycteris poeyi Gundlach, 1861; Pteronotus were identified as O. viguerasi following Cooley and macleayii Gray, 1839; Pteronotus quadridens Gund- Kohls (1941). Seventeen males were tentatively as- lach, 1840; Pteronotus parnellii Gray, 1843; Pterono- signed to O. viguerasi due to the presence of scle- tus gymnonotus Natterer, 1843; Pteronotus davyi Gray, rotized ventral plates, which is a diagnostic char- 1838; Brachyphylla nana Miller, 1902; Mormoops mega- acter of adults of the subgenus Subparmatus. Ad- lophylla Peters, 1864; Mormoops blainvillei Leach, ditionally, a fragment of circa 420-bp of the mito- 1821; Erophylla sezekorni Gundlach, 1861; and Ero- chondrial 16S rDNA gene was obtained from two phylla bombifrons Miller, 1899 (Kohls et al. 1965; males and one female in order to confirm the tax- Cerný and Dusbábek 1967; Cerný 1969; Tamsitt and onomic determination by morphological criteria. Fox 1970; Jones et al. 1972; De la Cruz and Abreu Both males and females were deposited at the Tick 1984; Kurta et al. 2007). Collection of the Instituto Nacional de Tecnología The description of Cooley and Kohls (1941) was Agropecuaria, Estación Experimental Agropecuaria based on a female, nymphs and larvae collected in Rafaela, Rafaela, Santa Fe, Argentina (INTA 2187), "Cueva Somorrostro", Cuba. Larvae were found at- at the Coleção Nacional de Carrapatos (CNC) of tached to bats, probably P. poeyi. Although Gugliel- the Faculdade de Medicina Veterinária e Zootecnia, mone et al. (2003) stated that all stages of O. viguerasi Universidade de São Paulo, São Paulo, Brazil (CNC were described, the description of adults by Coo- 1717) and in the Departamento de Parasitología Vet- ley and Kohls (1941) was based on only the female, erinaria, Facultad de Veterinaria, Universidad de la without inclusion of male specimens. Danielová et República, Montevideo, Uruguay (DPVURU 768). al. (1982) reported the finding of males and females Six males and six females were measured using of O. viguerasi on the walls of bat caves in Cuba, but a stereoscope Nikon® C-PS (all measurements are the methodology for tick determination was not ex- given in mm, the mean followed by the range in plained by these authors and the collection where parentheses). Scanning electron photomicrographs the ticks were deposited was not indicated. There- of males and females were taken at the Servicio fore, it can be affirmed that the male of this tick de Microscopía Electrónica, Museo de La Plata, species is not formally described. Universidad Nacional de La Plata, Argentina, us- The aim of this work was to carry out the de- ing a JEOL/JSM 6360 LV® Digital Scanning Mi- scription of males of O. viguerasi from specimens re- croscope, and Sub-Unidad de Microscopía Elec- cently collected from a bat cave in Mexico, and to trónica de Barrido, Facultad de Ciencias, Montev- compare the morphology of both males and females ideo, Uruguay, using JEOL JMS-5900 scanning elec- from Mexico with the morphology of the specimens tron microscope. The holotype female (RML17169), of O. viguerasi described by Cooley and Kohls (1941) the paratype nymph (RML17164) and other mate- and also with those deposited in the United States rial (RML50482, RML64683, RML17495, RML64680, National Tick Collection (USNTC, Georgia South- RML64679, RML64682, RML52459, RML17162,
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    Williams et al. Parasites & Vectors (2015) 8:90 DOI 10.1186/s13071-015-0704-x RESEARCH Open Access Fluralaner activity against life stages of ticks using Rhipicephalus sanguineus and Ornithodoros moubata IN in vitro contact and feeding assays Heike Williams*, Hartmut Zoller, Rainer KA Roepke, Eva Zschiesche and Anja R Heckeroth Abstract Background: Fluralaner is a novel isoxazoline eliciting both acaricidal and insecticidal activity through potent blockage of GABA- and glutamate-gated chloride channels. The aim of the study was to investigate the susceptibility of juvenile stages of common tick species exposed to fluralaner through either contact (Rhipicephalus sanguineus) or contact and feeding routes (Ornithodoros moubata). Methods: Fluralaner acaricidal activity through both contact and feeding exposure was measured in vitro using two separate testing protocols. Acaricidal contact activity against Rhipicephalus sanguineus life stages was assessed using three minute immersion in fluralaner concentrations between 50 and 0.05 μg/mL (larvae) or between 1000 and 0.2 μg/mL (nymphs and adults). Contact and feeding activity against Ornithodoros moubata nymphs was assessed using fluralaner concentrations between 1000 to 10−4 μg/mL (contact test) and 0.1 to 10−10 μg/mL (feeding test). Activity was assessed 48 hours after exposure and all tests included vehicle and untreated negative control groups. Results: Fluralaner lethal concentrations (LC50,LC90/95) were defined as concentrations with either 50%, 90% or 95% killing effect in the tested sample population. After contact exposure of R. sanguineus life stages lethal concentrations were (μg/mL): larvae - LC50 0.7, LC90 2.4; nymphs - LC50 1.4, LC90 2.6; and adults - LC50 278, LC90 1973.
  • Tick [Genome Mapping]

    Tick [Genome Mapping]

    University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Public Health Resources Public Health Resources 2008 Tick [Genome Mapping] Amy J. Ullmann Centers for Disease Control and Prevention, Fort Collins, CO Jeffrey J. Stuart Purdue University, [email protected] Catherine A. Hill Purdue University Follow this and additional works at: https://digitalcommons.unl.edu/publichealthresources Part of the Public Health Commons Ullmann, Amy J.; Stuart, Jeffrey J.; and Hill, Catherine A., "Tick [Genome Mapping]" (2008). Public Health Resources. 108. https://digitalcommons.unl.edu/publichealthresources/108 This Article is brought to you for free and open access by the Public Health Resources at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in Public Health Resources by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. 8 Tick Amy J. Ullmannl, Jeffrey J. stuart2, and Catherine A. Hill2 Division of Vector Borne-Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA Department of Entomology, Purdue University, 901 West State Street, West Lafayette, IN 47907, USA e-mail:[email protected] 8.1 8.1 .I Introduction Phylogeny and Evolution of the lxodida Ticks and mites are members of the subclass Acari Ticks (subphylum Chelicerata: class Arachnida: sub- within the subphylum Chelicerata. The chelicerate lin- class Acari: superorder Parasitiformes: order Ixodi- eage is thought to be ancient, having diverged from dae) are obligate blood-feeding ectoparasites of global Trilobites during the Cambrian explosion (Brusca and medical and veterinary importance. Ticks live on all Brusca 1990). It is estimated that is has been ap- continents of the world (Steen et al.
  • Quaternary Bat Diversity in the Dominican Republic

    Quaternary Bat Diversity in the Dominican Republic

    AMERICAN MUSEUM NOVITATES Number 3779, 20 pp. June 21, 2013 Quaternary Bat Diversity in the Dominican Republic PAÚL M. VELAZCO,1 HANNAH O’NEILL,2 GREGG F. GUNNELL,3 SIOBHÁN B. COOKE,4 RENATO RIMOLI,5 ALFRED L. ROSENBErgER,1, 6 AND NANCY B. SIMMONS1 ABSTRACT The fossil record of bats is extensive in the Caribbean, but few fossils have previously been reported from the Dominican Republic. In this paper, we describe new collections of fossil bats from two flooded caves in the Dominican Republic, and summarize previous finds from the Island of Hispaniola. The new collections were evaluated in the context of extant and fossil faunas of the Greater Antilles to provide information on the evolution of the bat community of Hispaniola. Eleven species were identified within the new collections, including five mormoopids (Mormoops blainvillei, †Mormoops magna, Pteronotus macleayii, P. parnellii, and P. quadridens), five phyllostomids (Brachy- phylla nana, Monophyllus redmani, Phyllonycteris poeyi, Erophylla bombifrons, and Phyllops falcatus), and one natalid (Chilonatalus micropus). All of these species today inhabitant Hispaniola with the exception of †Mormoops magna, an extinct species previously known only from the Quaternary of Cuba, and Pteronotus macleayii, which is currently known only from extant populations in Cuba and Jamaica, although Quaternary fossils have also been recovered in the Bahamas. Differences between the fossil faunas and those known from the island today suggest that dispersal and extirpa- tion events, perhaps linked to climate change or stochastic events such as hurricanes, may have played roles in structuring the modern fauna of Hispaniola. 1 Division of Vertebrate Zoology (Mammalogy), American Museum of Natural History.