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Biological and Molecular Properties of Sweet Potato Leaf Curl Virus. Pongtharin Lotrakul Louisiana State University and Agricultural & Mechanical College

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Biological and Molecular Properties of Sweet Potato Leaf Curl Virus. Pongtharin Lotrakul Louisiana State University and Agricultural & Mechanical College Louisiana State University LSU Digital Commons LSU Historical Dissertations and Theses Graduate School 2000 Biological and Molecular Properties of Sweet Potato Leaf Curl Virus. Pongtharin Lotrakul Louisiana State University and Agricultural & Mechanical College Follow this and additional works at: https://digitalcommons.lsu.edu/gradschool_disstheses Recommended Citation Lotrakul, Pongtharin, "Biological and Molecular Properties of Sweet Potato Leaf Curl Virus." (2000). LSU Historical Dissertations and Theses. 7374. https://digitalcommons.lsu.edu/gradschool_disstheses/7374 This Dissertation is brought to you for free and open access by the Graduate School at LSU Digital Commons. It has been accepted for inclusion in LSU Historical Dissertations and Theses by an authorized administrator of LSU Digital Commons. For more information, please contact [email protected]. INFORMATION TO USERS This manuscript has been reproduced from the microfilm m aster UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer. The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand comer and continuing from left to right in equal sections with small overlaps. Photographs included in the original manuscript have been reproduced xerographically in this copy. Higher quality 6” x 9” black and white photographic prints are available for any photographs or illustrations appearing in this copy for an additional charge. Contact UMI directly to order. Bell & Howell Information and Learning 300 North Zeeb Road, Ann Arbor, Ml 48106-1346 USA 800-521-0600 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. BIOLOGICAL AND MOLECULAR PROPERTIES OF SWEET POTATO LEAF CURL VIRUS A Dissertation Submitted to the Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College in partial fulfillment of the requirements for the degree of Doctor of Philosophy in The Department of Plant Pathology and Crop Physiology by Pongtharin Lotrakul B.S., Chulalongkom University, 1992 M.S., Mahidol University, 1995 December, 2000 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. UMI Number: 9998693 ___ ® UMI UMI Microform 9998693 Copyright 2001 by Bell & Howell Information and Learning Company. All rights reserved. This microform edition is protected against unauthorized copying under Title 17, United States Code. Bell & Howell Information and Learning Company 300 North Zeeb Road P.O. Box 1346 Ann Arbor, Ml 48106-1346 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. To Mom & Dad 11 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. ACKNOWLEDGEMENTS I would like to express my sincere gratitude to my major professor, Dr. R. A. Valverde, for his guidance, encouragement, patience, and friendship throughout my doctoral study at Louisiana State University. I could not accomplish this work without his support My appreciation is extended to Dr. C. A. Clark for his valuable advice. I would also like to thank the other members of my graduate committee, Drs. K. E. Damann, D. S. Shih, and C. E. Johnson for their constructive comments. I am grateful for the support that I had received from Dr. J. P. Snow. My special thank is extended to Mrs. Pat Hives for the generosity she gave me from my very first days at LSU. Additionally, appreciation is extended to the following people for their support and friendship: S. Jeonggu, A. D. Landry, E. R. de Souto, and Y.-K. Goh. I would also like to thank Dr. S. Hurtt from the APHIS Quarantine Laboratory, USDA, Beltsville, MD, for the Ipomoeasetosa DNA used in the genetic diversity study, M. W. Hoy for the information on virus detection, and Drs. M. M. Blackwell and S. O. Suh from the Department of Biological Sciences for their helpful advice in phylogenetic analysis. I gratefully acknowledge the financial support from the Ministry of University Affairs, Thailand, and LSU. Completion of my degree would not be possible without them. Finally, I would like to extend my sincere gratitude to my beloved parents, brother, and sister for their patience, understanding, and encouragement. I am deeply grateful for the unconditioned love that my parents have given me throughout my life. I could not have gone this far without them. iii Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. TABLE OF CONTENTS ACKNOWLEDGEMENTS........................................................................................ iii LIST OF TABLES.................................................................................................... v LIST OF FIGURES.................................................................................................. vi ABSTRACT.....................................................-............................................................ vii CHAPTER 1. INTRODUCTION........................................................................... 1 CHAPTER 2. REVIEW OF LITERATURE.......................................................... 3 2.1 Importance of Sweet Potato as Food Source .................................... 3 2.2 Physiology of Sweet Potato .............................................................. 4 2.3 Sweet Potato Viruses...........................................................................5 2.4 The Genus Begomovirus........................................................................ 18 2.5 Phylogenetic Relationships between Begomoviruses ...................... 24 2.6 The Emerging Begomoviruses...........................................................25 CHAPTER 3. DETECTION OF A GEMINIVIRUS INFECTING SWEET POTATO IN THE UNITED STATES.......................................... 28 3.1 Introduction ........................................................................................... 28 3.2 Materials and Methods ............................................................................... 29 3.3 Results......................................................................................................... 33 3.4 Discussion ................................................................................................ 36 CHAPTER 4. CLONING OF A DNA-A-LEKE GENOMIC COMPONENT OF SWEET POTATO LEAF CURL VIRUS : NUCLEOTIDE SEQUENCE AND PHYLOGENETIC RELATIONSHIPS 41 4.1 Introduction ........................................................................................... 41 4.2 Materials and Methods ...............................................................................42 4.3 Results......................................................................................................... 45 4.4 Discussion ................................................................................................ 58 CHAPTER 5. GENETIC DIVERSITY OF SWEET POTATO LEAF CURL V IRU S......................................................................................................69 5.1 Introduction ........................................................................................... 69 5.2 Materials and Methods ............................................................................... 70 5.3 Results.........................................................................................................72 5.4 Discussion ................................................................................................ 73 CHAPTER 6. SUMMARY AND CONCLUSION .................................................. 80 6.1 Summary ................................................................................................. 80 6.2 Conclusion ............................................................................................. 81 REFERENCES.............................................................................................................. 82 APPENDIX: LETTERS OF PERMISSION......................................................... 90 VITA ................................................................................................................................ 93 iv Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. LIST OF TABLES 3.1. Sweet potato whitefly ( Bemisia tabaci biotype B) transmission of the United States isolate of Sweet potato leaf curl virus after 2-day acquisition and transmission feeding periods .......................................... 34 4.1. Percent nucleotide sequence identity between DNA-A-like genomic component of the United States isolate of Sweet potato leaf curl virus and DNA-A or DNA-A-like genomic component of other begomoviruses 51 4.2. Percent nucleotide sequence identity and derived
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