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Supporting Information for Proteomics DOI 10.1002/Pmic.200600859

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Supporting Information for Proteomics DOI 10.1002/Pmic.200600859 Supporting Information for Proteomics DOI 10.1002/pmic.200600859 Daniela Fojo Seixas Chaves, Prcio Pereira Ferrer, Emanuel Maltempi de Souza, Leonardo Magalhes Gruz, Rose Adele Monteiro and Fbio de Oliveira Pedrosa A two-dimensional proteome reference map of Herbaspirillum seropedicae proteins ª 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim www.proteomics-journal.com Supplementary Table 1. Complete list of H. seropedicae identified proteins. Spot Accession IDa Numberb Protein name 1 CAM32534.1 Chaperone protein dnaK (HSP70) 2 CAM32674.1 Polyribonucleotide nucleotidyltransferase 3 CAM32559.1 Elongation factor G 1 (EF-G 1) 4 CAM32640.1 ATP-dependent Clp protease subunit (heat-shock) 5 CAM32585.1 30s ribosomal subunit protein S1 6 CAM32629.1 Dihydrolipoamide dehydrogenase 7 CAM32626.1 Catalase (peroxidase I) 8 CAM32583.1 NADP-dependent malic enzyme oxidoreductase 9 CAM32583.1 NADP-dependent malic enzyme oxidoreductase 10 CAM32547.1 NADH dehydrogenase I (Chain G) oxidoreductase 12 CAM32545.1 CTP synthase (UTP-ammonia lyase) 13 CAM32579.1 Phosphoenolpyruvate carboxykinase 14 CAM32603.1 Glutamine synthetase 15 CAM32607.1 Glutamine synthetase 16 CAM32690.1 Chaperonin GroEL (HSP60 family) 17 CAM32690.1 Chaperonin GroEL (HSP60 family) 18 CAM32550.1 Peptidyl-prolyl cis/trans isomerase (trigger factor) 19 CAM32670.1 F0F1-type ATP synthase, beta subunit 20 CAM32680.1 Histidinol dehydrogenase oxidoreductase 21 CAM32659.1 XAA-PRO aminopeptidase 22 CAM32670.1 F0F1-type ATP synthase, beta subunit 23 CAM32546.1 Enolase 24 CAM32546.1 Enolase 25 CAM32610.1 Succinyl-CoA synthetase protein, beta chain 26 CAM32610.1 Succinyl-CoA synthetase protein, beta chain 26 CAM32569.1 Thioredoxin reductase oxidoreductase 28 CAM32678.1 SAM-dependent methyltransferase 29 CAM32556.1 Conserved hypothetical protein 30 CAM32651.1 Sugar kinase ribokinase family 31 CAM32688.1 NADPH:quinone oxidoreductase 32 CAM32688.1 NADPH:quinone oxidoreductase 33 CAM32668.1 Probable DNA-directed RNA polymerase (alpha chain) 34 CAM32665.1 Actin-like ATPase involved in cell morphogenesis 35 CAM32604.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 37 CAM32549.1 FKBP-type peptidyl-prolyl cis-trans isomerase (Rotamase) 38 CAM32575.1 Elongation factor TS (EF-TS) 40 CAM32595.1 Xanthine dehydrogenase (subunit A) 40 CAM32643.1 3-isopropylmalate dehydrogenase oxidoreductase 40 CAM32571.1 Flagellin protein; filament structural 41 CAM32590.1 GTPase translation elongation factor TU (EF-Tu) 42 CAM32557.1 S-adenosylmethionine synthetase 43 CAM32667.1 Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex 44 CAM32631.1 Argininosuccinate synthase 46 CAM32540.1 Seryl-tRNA synthetase 47 CAM32660.1 Parvulin-like peptidyl-prolyl cis-trans isomerase 48 CAM32536.1 Phosphoribosylaminoimidazolecarboxamide formyltransferase and IMP cyclohydrolase transmembrane 48 CAM32648.1 Fumarate hydratase 49 CAM32535.1 Electron transfer flavoprotein-ubiquinone oxidoreductase 50 CAM32677.1 F0F1-type ATP synthase, alpha subunit 52 CAM32562.1 Glutamate synthase (small subunit) oxidoreductase 53 CAM32573.1 2-oxoglutarate dehydrogenase, E3 component, lipoamide dehydrogenase 54 CAM32592.1 Adenylosuccinate synthetase 55 CAM32650.1 Biotin carboxylase 56 CAM32565.1 Tartrate dehydrogenase 57 CAM32635.1 Glutamate-1-semialdhyde 2,1-aminomutase 58 CAM32565.1 Tartrate dehydrogenase 59 CAM32688.1 NADPH:quinone oxidoreductase 60 CAM32623.1 Galactose-1-phosphate uridylyltransferase 61 CAM32601.1 Sulfate adenylyltransferase subunit 2 62 CAM32612.1 Fructose-1,6-bisphosphate aldolase 63 CAM32598.1 Acetylornithine aminotransferase 65 CAM32553.1 HlyD family membrane-fusion 66 CAM32644.1 Acetyl-CoA acyltransferase 67 CAM32632.1 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase 68 CAM32581.1 ABC-type proline/glycine betaine transport system, ATPase component 68 CAM32622.1 UDP-N-acetylglucosamine 2-epimerase 69 CAM32621.1 Aryl-alcohol dehydrogenase 70 CAM32560.1 Phosphoribosylpyrophosphate synthetase 71 CAM32686.1 Fructose-1,6-bisphosphatase 72 CAM32701.1 Malate dehydrogenase 73 CAM32630.1 L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D 74 CAM32621.1 Aryl-alcohol dehydrogenase 75 CAM32701.1 Malate dehydrogenase 76 CAM32621.1 Aryl-alcohol dehydrogenase 77 CAM32621.1 Aryl-alcohol dehydrogenase 78 CAM32645.1 Acetyl-CoA acetyltransferase (acetoacetyl-CoA thiolase) 79 CAM32645.1 Acetyl-CoA acetyltransferase (acetoacetyl-CoA thiolase) 80 CAM32599.1 Septum site-determining 81 CAM32613.1 Phosphoribosylaminoimidazole-succinocarboxamide synthase 82 CAM32542.1 Inositol monophosphatase (extragenic suppressor) 83 CAM32663.1 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase 84 CAM32654.1 Sugar phosphate isomerase (involved in capsule formation) 85 CAM32552.1 Pantoate-beta-alanine ligase (panthothenate synthetase) 86 CAM32663.1 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase 88 CAM32611.1 Succinyl-CoA synthetase alpha chain 90 CAM32611.1 Succinyl-CoA synthetase alpha chain 91 CAM32558.1 Acetylglutamate kinase 92 CAM32694.1 Probable universal stress proten 93 CAM32694.1 Probable universal stress protein 94 CAM32703.1 Succinate dehydrogenase Fe-S protein subunit protein 95 CAM32565.1 Tartrate dehydrogenase 95 CAM32597.1 ATPase with chaperone activity, ATP-binding subunit 96 CAM32629.1 Dihydrolipoamide dehydrogenase (E3 component of pyruvate and 2- oxoglutarate dehydrogenases complexes) Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide 97 CAM32648.1 isomerase 98 CAM32661.1 Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit protein ABC-type proline/glycine betaine transport system, periplasmic component 99 CAM32580.1 100 CAM32683.1 Translation elongation factor P (EF-P)/translation initiation factor 5A (eIF- 5A) 102 CAM32616.1 Heat shock protein 24 (HSP-70 cofactor) 103 CAM32591.1 Inorganic pyrophosphatase 105 CAM32682.1 Transmembrane ubiquinol-cytochrome C reductase (Iron-sulfur subunit) oxidoreductase 106 CAM32624.1 Hypothetical protein 107 CAM32618.1 Dihydrodipicolinate reductase CAM32657.1 Conserved hypothetical protein; Predicted periplasmic or secreted 108 lipoprotein 109 CAM32602.1 Carboxyphosphonoenolpyruvate phosphonomutase 110 CAM32619.1 Protein-L-isoaspartate O-methyltransferase 111 CAM32614.1 Pyridoxamine 5`-phosphate oxidase 112 CAM32664.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase 113 CAM32539.1 Peroxiredoxin 114 CAM32661.1 Acyl CoA:acetate/3-ketoacid CoA transferase, alpha subunit 117 CAM32605.1 Adenylate kinase (ATP-AMP transphosphorylase) 118 CAM32698.1 Acetoacetyl-CoA reductase 120 CAM32564.1 Phasin family protein 125 CAM32538.1 Probable ABC-type glutamate/aspartate transport system, ATPase component 126 CAM32539.1 Peroxiredoxin 128 CAM32688.1 NADPH:quinone oxidoreductase 129 CAM32606.1 Superoxide dismutase [Fe] 130 CAM32698.1 Acetoacetyl-CoA reductase 131 CAM32673.1 Methyl-accepting chemotaxis 137 CAM32551.1 ATP-dependent Clp protease (proteolytic subunit) 140 CAM32544.1 Transcription elongation factor 142 CAM32671.1 F0F1-type ATP synthase, epsilon subunit (mitochondrial delta subunit) 143 CAM32669.1 DnaK suppressor 145 CAM32554.1 Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family 146 CAM32702.1 Polybetahydroxybutyrate accumulation regulatory (PhbF) 148 CAM32600.1 Conserved hypothetical protein 149 CAM32615.1 Signal peptide 151 CAM32653.1 Single-strand DNA-binding protein (helix-destabilizing protein) 153 CAM32574.1 Transmembrane protein 155 CAM32639.1 S-adenosylmethionine:2-demethylmenaquinone methyltransferase 157 CAM32596.1 FOG: CBS domain containing 158 CAM32609.1 FKBP-type peptidyl-prolyl cis-trans isomerase I 160 CAM32593.1 Conserved hypothetical protein 162 CAM32543.1 Glycolate/propanediol utilization 163 CAM32689.1 Co-chaperonin GroES (HSP10) 164 CAM32572.1 Nucleoside-diphosphate kinase 165 CAM32578.1 Universal stress protein 166 CAM32567.1 Nitrogen regulatory P-II protein 168 CAM32693.1 Conserved hypothetical protein 171 CAM32617.1 Ferric uptake regulator 174 CAM32548.1 Conserved hypothetical protein 176 CAM32652.1 Probable exported protein 178 CAM32655.1 Hypothetical protein Phosphopantetheine adenylyltransferase 179 CAM32676.1 180 CAM32679.1 UDP-N-acetylglucosamine pyrophosphorylase 181 CAM32675.1 Zinc protease-like signal peptide 182 CAM32581.1 ABC-type proline/glycine betaine transport system, ATPase component 183 CAM32612.1 Fructose-1,6-bisphosphate aldolase 184 CAM32697.1 Beta-hydroxybutyrate dehydrogenase 189 CAM32570.1 PKHD-type hydroxylase 190 CAM32555.1 Acetyl-coenzyme A carboxylase carboxyl transferase (alpha subunit) 191 CAM32611.1 Succinyl-CoA synthetase alpha chain 192 CAM32537.1 Conserved hypothetical protein 192 CAM32566.1 Probable ubiquinone biosynthesis protein COQ7 194 CAM32590.1 GTPase translation elongation factor TU (EF-Tu) 195 CAM32628.1 Probable HSP90 family high temperature molecular chaperone G 196 CAM32633.1 Glyceraldehyde 3-phosphate/erythrose-4-phosphate dehydrogenase 197 CAM32563.1 Electron transfer flavoprotein, alpha subunit 198 CAM32594.1 Aspartate-semialdehyde dehydrogenase 199 CAM32541.1 Hydrolase or acyltransferase 200 CAM32614.1 Pyridoxamine 5`-phosphate oxidase 201 CAM32587.1 GMP synthase 202 CAM32646.1 DNA-binding ferritin-like protein (oxidative damage protectant) 205 CAM32577.1 UTP-glucose-1-phosphate uridylyltransferase 206 CAM32582.1 TetR-family transcriptional regulator 207 CAM32647.1 Thiamine monophosphate synthase 209 CAM32685.1 Conserved hypothetical protein 211 CAM32625.1 Imidazoleglycerol-phosphate synthase 213 CAM32568.1 NAD+ dependent
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