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RESEARCH REPORT RESEARCH REPORT February 2013 Atlantic Sourced Shells as Alternative Calcium Sources for for Laying Hens Introduction: Currently ground limestone and large particle shell are the common sources of calcium for laying hens providing both small and large particle sizes in the diet. However, the price of oyster shell is approximately 3 times that of limestone. In Atlantic Canada, over 5000 tonnes of blue shells and over 6000 tonnes of are produced annually. By-product from these industries consists of broken and crushed shells and undersized .

Atlantic Sourced Shells:

Blue edulis Surf – Spisula solidissima Common --( undatum) Objective: The objective of each project was to determine the effect of replacing traditional oyster shell and ground limestone with large and fine ground , surf clam or common whelk shell on production performance throughout short and long-term replacement periods. Methods and Materials: Three trials were conducted and each trial used 240 White Lohmann Lite Laying hens (20-25wks of age). Experimental diets were formulated to meet or exceed breeder nutrient specifications.

Table 1: Commercial and Alternate Calcium Sources for each of the Three Laying hen Research Trials Trial 1 Trial 2 Trial 3 Common -1/3 large particle oyster shell + -1/3 large particle oyster shell + -1/3 large particle oyster shell + Commercial 2/3 ground limestone (OS/GL) 2/3 ground limestone: (OS/GL) 2/3 ground limestone (OS/GL) Sources -1/3 Shell mix + 2/3 ground -1/3 Shell mix + 2/3 ground limestone (SM/GL) limestone (SM/GL) -Ground Limestone (GL) -Ground limestone (GL) Alternative -1/3 blue mussel shells + 2/3 -1/3 blue mussel shells + 2/3 -1/3 large particle whelk shells + Calcium ground limestone (BMS/GL) ground blue mussel shells 2/3 ground whelk (W/GW) Sources (BMS/GBMS) -1/3 clam shells + 2/3 ground -Ground blue mussel shells -100% ground whelk shells limestone (CS/GL) (GBMS) (GW)

Results: Trial 1- Surf Clam and Blue Mussel: This was a short cycle trial, 8 weeks in duration. Calcium source did not have an effect (P>0.05) on hen- day production (Fig.1) feed consumption, or body weights. At 26 weeks of age, eggs from hens fed the OS/GL diet had lower (P≤0.05) specific gravity. At 28 weeks of age, the specific gravity of eggs from hens fed the SM/GL diet was higher (P≤0.05) than Fig 1. Trial 1 Hen-day production 150 the specific gravities of eggs from hens fed the other diets. The amount of force required to break 100 the eggshells was not affected (P>0.05) by

% calcium source. 50 Conclusions: Large particle blue mussel and surf 0 clam shells were successful replacements for 20 21 22 23 24 25 26 27 28 oyster during a short-term production cycle. Weeks of Age OS/GL SM/GL BMS/GL CS/GL

Trial 2- Blue Mussel: This was a 52 week study. Calcium source did not have an effect on hen-day production (Fig. 2), feed consumption, egg specific gravity, egg breaking strength, or body weights for the duration of the trial. Egg weights did not differ (P>0.05) among the treatments except at the end of the trial. Eggs laid by hens fed the GBM treatment weighed less (P≤0.05) (64.6 g) than those from hens fed the OS/GL (66.3 g) and BM/GBM (67.3 g) during the last period only. Although there was a difference, mean Fig 2.Trial 2 Hen-day Production 150 egg weights at 77 weeks of age were all in the extra-large category. 100

% Conclusions: Large and finely ground blue 50 mussel shells were successful replacements 0 for oyster shells and finely ground limestone during a full-term production cycle. Weeks of Age OS/GL GL SM/GL BM/GBM GBM

Trial 3- Whelk: Feed consumption (Fig.3), body weights, and hen-day production (Fig.4) were similar regardless of calcium source up until 64 weeks. From 65-72 weeks temperature in the facility increased (Fig.5) which ay have resulted in decreased feed consumption, body weights and hen-day production for birds fed W/GW and GW over birds fed OS/GL. Egg weight, egg specific gravity, and shell breaking strength were not affected (P>0.05) by treatment during the trial. Conclusion: Production data up to 64 weeks of age were similar, however, performance was reduce from 65-76 wks of age for birds fed ground whelks, potentailly due to increased environmental temperatures.

Fig 3. Trial 3 Feed ConsumptionA 150 Fig 4. Trial 3 Hen-day production Fig 5. Trial 3 Air ) B

1 A 150 - BB Temperature day A A A

1 - A AB AB 25 B AB AB 100 B B B 100 A A C) A A A ° 24 AB A AB B % B B B 23 50 50 22 21 0 0 ( Temperature

20

Feed Consumption Feed Consumption (gbird

25-28 29-32 33-36 37-40 41-44 45-48 49-52 53-56 57-60 61-64 65-68 69-72 73-76

21-24 61-64 65-68 69-72 73-74

21-24 25-28 29-32 33-36 37-40 41-44 45-48 49-52 53-56 57-60 61-64 65-68 69-72 73-76 Weeks of Age Weeks of Age Weeks of Age OS/GL GL OS/GL GL W/GW GW

Prepared by: Derek M. Anderson, Janice MacIsaac and Ashley Gillcrist Funds provided by: NS Dept. of Agriculture, Nova Scotia Egg Producers, PEI ADAPT Council, PEI Processors Assoc., St. Laurent Gulf Products, PEI Dept. of Agriculture, Marine Institute of Memorial University, Cape Mariner Enterprises, ACOA, NRC, CCFI, Nfld & Labr. Dept. of Fisheries and .