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Rate Constants for Reaction of Hydroxyl Radicals with Tris, Tricine and Hepes Buffers

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Rate Constants for Reaction of Hydroxyl Radicals with Tris, Tricine and Hepes Buffers View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector Volume 199, number 1 FEBS 3563 April 1986 Rate constants for reaction of hydroxyl radicals with Tris, Tricine and Hepes buffers Mark Hicks and Janusz M. Gebicki* School of Biological Sciences, Macquarie University, North Ryde, 2113 NSW, Australia Received 21 January 1986 Rate constants for the reactions of three commonly used organic buffers and hydroxyl radicals were mea- sured using steady-state competition kinetics with thymine. For Hepes (4-(2-hydroxyethyl)-l-piperazine- ethanesulphonic acid), Tricine (N-[2-hydroxy-l,l-bis(hydroxymethyl)ethyl]glycine and Tris (2-amino-2- hydroxymethylpropane-1,3-diol) the rate constants were 5.1 x 109, 1.6 x lo9 and 1.1 x lo9 I .rnol-‘. SK’, respectively. Hydroxyl radical Organic buffer Hydroxyl radical scavenger Rate constant 1. INTRODUCTION essential in stabilizing the membranes [7], a separate study of its reactivity towards the hydrox- Since their introduction by Good et al. [l], a yl radical (HO’) was undertaken. Earlier reports range of organic buffers have been widely [8-l l] have indicated that Tris can scavenge HO’ employed because of their efficient buffering radicals, but the rate constant for the reaction was capacity near physiological pH. In addition, de- not measured. We now report the rate constants tailed studies with soluble or membrane-bound en- for the .reactions of HO’ with Tris, Tricine and zymes and cultured cell lines have shown these buf- Hepes. The values were obtained by steady-state fers to be often superior to inorganic buffers such competition kinetics with thymine. as phosphate, borate or bicarbonate in protecting many systems from denaturation [l-3]. The 2. MATERIALS AND METHODS organic buffers have also been used in examina- tions of the formation and effects of free radicals Tris, Tricine and Hepes were AR grade (Ultrol) in biological systems [4-61. and obtained from Calbiochem. Thymine was pur- In the course of a study of the effects of free chased from Sigma and recrystallized twice from radicals on fatty acid membranes, it became clear hot triply distilled water and EDTA before use. All that the presence of Tris complicated the inter- other chemicals were of AR grade from Ajax pretation of our results, because of its potential to Chemicals. Water used for solution preparation scavenge hydroxyl radicals. Since this buffer is was distilled then passed through a Mini-Q water purification system equipped with an Organex-Q cartridge for removal of trace organic residues. Irradiations were performed in a 2500 Ci ‘j°Co * To whom correspondence should be addressed gamma source. Dose rates were measured by Abbreviations: Hepes, 4-(2-hydroxyethyl)-l-piperazine- Fricke dosimetry [12] with the G value for the for- ethanesulphonic acid; Tricine, N-[2-hydroxy-l,l-bis- mation of Fe3+ taken as 15.5 ions/100 eV [13]. The (hydroxymethyl)ethyl]glycine; Tris, 2-amino-2-hydroxy- dose rates varied from 40 to 60 Gy/min. LOSS of methylpropane-l,3-diol thymine was measured as the decrease in ab- Published by Elsevier Science Publishers B. V. (Biomedical Division) 92 00145793/86/$3.50 0 1986 Federation of European Biochemical Societies Volume 199, number 1 FEBS LETTERS April 1986 sorbance at 264 nm with the molar extinction coef- ficient of thymine taken as 7900 1. mol-’ *cm-’ [141. 3. RESULTS AND DISCUSSION Systems consisting of two solutes capable of reacting at comparable speeds with a third species are commonly used to measure reaction rate con- stants in solution. For such competing processes, the extent of each reaction is proportional to its in- dividual rate constant. Here, the competing solutes were thymine (T) and a buffer (B), while the reac- tive species was the HO’ radical generated by radiolysis of water. The reactions were thus: [BUFFER] (mM) kT Fig.2. Decrease in radiation-induced loss of thymine HO’ + T - T-products chromophore as a function of buffer concentration. Each point is the mean of 5 determinations; the error ks bars are + SE. (0) Hepes, (0) Tricine, (A) Tris. Dose HO’ + B - B-products rate was 42.25 Gy/min for Hepes and Tricine and 60 Gy/min for Tris. It is convenient to measure radiation chemical yields in terms of ‘G values’. These give the In the experiments, [T] and [B] are arranged as re- number of molecules of any species formed ( + ) or quired, the value of kT is taken from the literature, destroyed ( -) for every 100 eV of energy ab- G( - T) is measured by following the loss of absor- sorbed. The loss of thymine chromophore on ex- bance of irradiated thymine and the effective posure to radiolytically produced HO’ is given by: G(H0’) determined from the G( - T) at zero buf- fer concentration. Thus, only the change in the kT[T] concentration of one radical scavenger needs to be G( - T) = G(H0’) x kT[T] + kB[B] followed. When G( - T) is determined for a 06 a 1 \ IRRADIATION TIME (mm) Fig.1. Loss of thymine chromophore in different Fig.3. Reciprocal of G( - thymine) as a function of the thymine/Hepes mixtures as a function of absorbed initial bufferkhymine ratios. (0) Hepes, (0) Tricine, radiation dose. Dose rate 42.25 Gy/min. Initial (A) Tris. Correlation coefficients (2 values) for the [thymine] = 0.09 mM. Initial (Hepes]: (0) 0, (0) 0.09. equations of the lines are 0.998, 0.991 and 0.998 for (A) 0.18, (A) 0.27 mM. Hepes, Tricine and Tris, respectively. 93 Volume 199, number 1 FEBS LETTERS April 1986 number of buffer/thymine ratios, kg can be Australian Research Grants Scheme for their calculated from the slope of the plot of (G( - T))-’ financial support. One of us (M.H.) was supported vs [B]/[T]. by an Australian Commonwealth Postgraduate Aerated solutions of thymine (0.09 mM) in Research Award. 6.6 mM Na2HPOs/KH2P04 buffer at pH 8.0 were irradiated. The G( - T) values were independent of REFERENCES initial thymine concentrations at around 0.1 mM 111 Good, N.E., Winget, G.D., Winter, W., Connolly, and above, indicating complete scavenging of the T.N., Izawa, S. and Singh, M.M. (1966) HO’ radicals. Fig.1 shows the effect of increasing Biochemistry 5, 467-477. concentration of Hepes on the loss of the thymine PI Good, N.E. and Izawa, S. (1972) Methods chromophore. Clearly, the buffer protected Enzymol. 24B, 53-68. thymine by competing for the HO’ radicals. [31 Ferguson, W.F., Braunschweiger, K.I., Similar results (not shown) were obtained with Tris Braunschweiger, W.R., Smith, J.R., McCormick, and Tricine. The effect of buffer concentration on J. J., Wasmann, C.C., Jarvis, N.P., Bell, D.H. and Good, N.E. (1980) Anal. Biochem. 104, 300-310. the disappearance of thymine chromophore is Greenwald, R.A. and Moy, W.W. (1980) Arthritis shown in fig.2. In the absence of buffer, G( - T) I41 Rheum. 23, 455-463. varied between I .8 and 2. I, with an average of I .9. PI Burk, R.F. (1983) Biochim. Biophys. Acta 757, Myers et al. [15] found similar variability. The G 21-28. value of 1.9 shows that not all the HO’ radicals 161 Yukawa, O., Nagatsuka, S. and Nazawa, T. (1983) generated reacted to saturate the 5-6 position of Int. J. Radiat. Biol. 43, 391-398. the thymine ring, because G(H0’) is 2.8 [16]. This 171 Gebicki, J.M. and Hicks, M. (1976) Chem. Phys. agrees with the finding of several products derived Lipids 16, 142-160. from thymine/HO’ reactions [151. WI Paschen, W. and Weser, U. (1975) Hoppe-Seyler’s The data from fig.2 were transformed to give the Z. Physiol. Chem. 3568, 727-737. Finkelstein, E., Rosen, G.M. and Rauckman, E. J. results plotted in fig.3. Values of the rate constants 191 (1980) Arch. Biochem. Biophys. 200, l-16. were calculated from the relationship: slope = WI Saprin, A.N. and Piette, L.H. (1977) Arch. Bio- kdG(HO’)kT using the absolute value of kT = them. Biophys. 180, 480-492. 4.7 x lo9 M-' *s-r[17]. The results were: for 1111 Tien, M., Svingen, B.A. and Aust, S.D. (1981) Hepes, 5.1 x 109; for Tricine, 1.6 x 109; for Tris Fed. Proc. 40, 179-182. 1.1 x lo9 (all in M-‘es-‘). 1121 Weiss, J., Allen, A.O. and Schwartz, W.A. (1955) This study demonstrates that Hepes, Tricine and Proc. 1st Int. Conf. Peaceful Uses Atomic Energy Tris are efficient scavengers of HO’ radicals. It 14, 179-181. is likely that other organic buffers also act 1131 Allen, A.O. (1961) Radiation Chemistry of Water as scavengers with rate constants around and Aqueous Solutions, p.22, Van Nostrand, New York. lo9 M-r as-l. The finding that under some condi- 1141 Scholes, G. and Willson, R.L. (1967) Trans. tions not all the HO’ radical damage is inhibited by Faraday Sot. 63, 2983-2993. these buffers (4-6,181 suggests either that the [I51 Myers, L.S., Ward, J.F., Tsukamoto, W.T., secondary radicals derived from the buffers can in- Holmes, D.E. and Julca, J.R. (1965) Science 148, itiate damage or that the HO’ radicals are pro- 1234-1235. duced at sites inaccessible to the buffer molecules. [I61 O’Donnell, J.H. and Sangster, D.F. (1970) There is a growing body of evidence for ‘site- Principles of Radiation Chemistry, p.82, Edward specific’ formation of HO’ which is not affected Arnold, London, by scavengers present in the aqueous phase [19,201.
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